YAL069W 1 335 649 OFF 1 YAL069W (335..649) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL068W-A 1 538 792 OFF 1 YAL068W-A (538..792) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL068C 0 1807 2169 ON 1 PAU8 complement(1807..2169) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YAL067W-A 1 2480 2707 OFF 1 YAL067W-A (2480..2707) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL067C 0 7235 9016 ON 1 SEO1 complement(7235..9016) Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide YAL066W 1 10091 10399 OFF 1 YAL066W (10091..10399) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL065C 0 11565 11951 ON 1 YAL065C complement(11565..11951) Putative protein of unknown function; has homology to FLO1; possible pseudogene YAL064W-B 1 12046 12426 ON 1 YAL064W-B (12046..12426) Fungal-specific protein of unknown function YAL064C-A 0 13363 13743 ON 1 TDA8 complement(13363..13743) Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene YAL064W 1 21566 21850 ON 1 YAL064W (21566..21850) Protein of unknown function; may interact with ribosomes, based on co-purification experiments YAL063C-A 0 22395 22685 OFF 1 YAL063C-A complement(22395..22685) Putative protein of unknown function; identified by expression profiling and mass spectrometry YAL063C 0 24000 27968 ON 1 FLO9 complement(24000..27968) Lectin-like protein with similarity to Flo1p, thought to be expressed and involved in flocculation YAL062W 1 31567 32940 ON 1 GDH3 (31567..32940) NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources YAL061W 1 33448 34701 ON 1 BDH2 (33448..34701) Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 YAL060W 1 35155 36303 ON 1 BDH1 (35155..36303) NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source YAL059C-A 0 36496 36918 OFF 1 YAL059C-A complement(36496..36918) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM1/YAL059W YAL059W 1 36509 37147 ON 1 ECM1 (36509..37147) Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm YAL058W 1 37464 38972 ON 1 CNE1 (37464..38972) Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast YAL056C-A 0 38696 39046 OFF 1 YAL056C-A complement(38696..39046) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL056W 1 39259 41901 ON 1 GPB2 (39259..41901) Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; homolog of Gpb1p YAL055W 1 42177 42719 ON 1 PEX22 (42177..42719) Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation YAL054C 0 42881 45022 ON 1 ACS1 complement(42881..45022) Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions YAL053W 1 45899 48250 ON 1 FLC2 (45899..48250) Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance YAL051W 1 48564 51707 ON 1 OAF1 (48564..51707) Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis YAL049C 0 51855 52595 ON 1 AIM2 complement(51855..52595) Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor YAL048C 0 52801 54789 ON 1 GEM1 complement(52801..54789) Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases YAL047W-A 1 54584 54913 OFF 1 YAL047W-A (54584..54913) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL047C 0 54989 56857 ON 1 SPC72 complement(54989..56857) Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization YAL046C 0 57029 57385 ON 1 AIM1 complement(57029..57385) Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss YAL045C 0 57488 57796 OFF 1 YAL045C complement(57488..57796) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A YAL044W-A 1 57518 57850 ON 1 YAL044W-A (57518..57850) Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein YAL044C 0 57950 58462 ON 1 GCV3 complement(57950..58462) H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF YAL043C 0 58695 61052 ON 1 PTA1 complement(58695..61052) Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII YAL042C-A 0 61231 61608 OFF 1 YAL042C-A complement(61231..61608) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene YAL042W 1 61316 62563 ON 1 ERV46 (61316..62563) Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport YAL041W 1 62840 65404 ON 1 CDC24 (62840..65404) Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing YAL040C 0 65778 67520 ON 1 CLN3 complement(65778..67520) G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis YAL039C 0 68716 69525 ON 1 CYC3 complement(68716..69525) Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) YAL038W 1 71786 73288 ON 1 CDC19 (71786..73288) Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration YAL037C-B 0 72326 73300 OFF 1 YAL037C-B complement(72326..73300) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL037C-A 0 73426 73518 OFF 1 YAL037C-A complement(73426..73518) Putative protein of unknown function YAL037W 1 74020 74823 ON 1 YAL037W (74020..74823) Putative protein of unknown function YAL036C 0 75043 76152 ON 1 RBG1 complement(75043..76152) Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid YAL035W 1 76427 79435 ON 1 FUN12 (76427..79435) GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 YAL034C-B 0 79489 79842 OFF 1 YAL034C-B complement(79489..79842) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL034W-A 1 79718 80587 ON 1 MTW1 (79718..80587) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly YAL034C 0 80710 81951 ON 1 FUN19 complement(80710..81951) Non-essential protein of unknown function; expression induced in response to heat stress YAL033W 1 82706 83227 ON 1 POP5 (82706..83227) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YAL032C 0 83335 84474 ON 1 PRP45 complement(83335..84474) Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene YAL031W-A 1 84669 84977 OFF 1 YAL031W-A (84669..84977) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL031C 0 84749 87031 ON 1 GIP4 complement(84749..87031) Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate YAL030W 1 87286 87752 ON 1 SNC1 (87286..87387,87501..87752) Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins YAL029C 0 87855 92270 ON 1 MYO4 complement(87855..92270) One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p YAL028W 1 92900 94486 ON 1 FRT2 (92900..94486) Tail-anchored ER membrane protein, interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate YAL027W 1 94687 95472 ON 1 SAW1 (94687..95472) Protein involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YAL026C-A 0 95386 95823 OFF 1 YAL026C-A complement(95386..95823) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAL027W and the verified gene DRS2 YAL026C 0 95630 99697 ON 1 DRS2 complement(95630..99697) Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Scer_HRA1 1 99305 99868 OFF 1 HRA1 (99305..99868) Non-protein-coding RNA, substrate of RNase P, possibly involved in rRNA processing, specifically maturation of 20S precursor into the mature 18S rRNA YAL025C 0 100225 101145 ON 1 MAK16 complement(100225..101145) Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus YAL024C 0 101565 105872 ON 1 LTE1 complement(101565..105872) Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures YAL023C 0 106272 108551 ON 1 PMT2 complement(106272..108551) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein Ser/Thr residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5; target for new antifungal YAL022C 0 108877 110430 ON 1 FUN26 complement(108877..110430) Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis YAL021C 0 110846 113359 ON 1 CCR4 complement(110846..113359) Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening YAL020C 0 113614 114615 ON 1 ATS1 complement(113614..114615) Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle YAL019W-A 1 114250 114819 OFF 1 YAL019W-A (114250..114819) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL019W 1 114919 118314 ON 1 FUN30 (114919..118314) Conserved member of the Snf2p family with ATP-dependent chromatin remodeling activity; has a role in silencing; potential Cdc28p substrate; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies YAL018C 0 118564 119541 ON 1 YAL018C complement(118564..119541) Putative protein of unknown function YAL017W 1 120225 124295 ON 1 PSK1 (120225..124295) One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status YAL016C-B 0 124307 124492 OFF 1 YAL016C-B complement(124307..124492) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL016C-A 0 124755 125069 OFF 1 YAL016C-A complement(124755..125069) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL016W 1 124879 126786 ON 1 TPD3 (124879..126786) Regulatory subunit A of the heterotrimeric protein phosphatase 2A (PP2A), which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III YAL015C 0 126903 128102 ON 1 NTG1 complement(126903..128102) DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress YAL014C 0 128252 129019 ON 1 SYN8 complement(128252..129019) Endosomal SNARE related to mammalian syntaxin 8 YAL013W 1 129270 130487 ON 1 DEP1 (129270..130487) Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation YAL012W 1 130799 131983 ON 1 CYS3 (130799..131983) Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine YAL011W 1 132199 134076 ON 1 SWC3 (132199..134076) Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae YAL010C 0 134184 135665 ON 1 MDM10 complement(134184..135665) Subunit of both the ERMES complex that links the ER to mitochondria, and of the mitochondrial sorting and assembly machinery (SAM complex) that functions in import and assembly of outer membrane beta-barrel proteins YAL009W 1 135854 136633 ON 1 SPO7 (135854..136633) Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation YAL008W 1 136914 137510 ON 1 FUN14 (136914..137510) Mitochondrial protein of unknown function YAL007C 0 137698 138345 ON 1 ERP2 complement(137698..138345) Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Scer_1.trna1P 1 139152 139254 ON 1 tP-UGG (139152..139187,139219..139254) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 60.49 YAL005C 0 139503 141431 ON 1 SSA1 complement(139503..141431) ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall; 98% identical with Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes YAL004W 1 140760 141407 OFF 1 YAL004W (140760..141407) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C YAL003W 1 142174 143160 ON 1 EFB1 (142174..142253,142620..143160) Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Scer_snR18 1 142367 142468 OFF 1 SNR18 (142367..142468) C/D box small nucleolar RNA (snoRNA); commonly referred to as U18; guides 2'-O-methylation of large subunit (LSU) rRNA at positions A649 and C650 YAL002W 1 143707 147531 ON 1 VPS8 (143707..147531) Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif YAL001C 0 147594 151166 ON 1 TFC3 complement(147594..151006,151097..151166) Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Scer_CEN1 1 151465 151582 ON 1 CEN1 (151465..151582) Chromosome I centromere YAR002W 1 152257 153876 ON 1 NUP60 (152257..153876) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 YAR002C-A 0 154065 154724 ON 1 ERP1 complement(154065..154724) Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles YAR003W 1 155005 156285 ON 1 SWD1 (155005..156285) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 YAR007C 0 156754 158619 ON 1 RFA1 complement(156754..158619) Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination YAR008W 1 158966 159793 ON 1 SEN34 (158966..159793) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Scer_1.trna2A 1 166267 166339 ON 1 tA-UGC (166267..166339) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: UGC | Cove Score: 76.57 YAR014C 0 166742 168871 ON 1 BUD14 complement(166742..168871) Protein involved in bud-site selection, Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YAR015W 1 169375 170295 ON 1 ADE1 (169375..170295) N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine YAR018C 0 170396 171703 ON 1 KIN3 complement(170396..171703) Nonessential serine/threonine protein kinase; possible role in DNA damage response YAR019C 0 172211 175135 ON 1 CDC15 complement(172211..175135) Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II YAR019W-A 1 174998 175330 OFF 1 YAR019W-A (174998..175330) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR020C 0 176856 177023 ON 1 PAU7 complement(176856..177023) Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme YAR023C 0 179281 179820 ON 1 YAR023C complement(179281..179820) Putative integral membrane protein, member of DUP240 gene family Scer_1.trna3L 1 181141 181254 ON 1 tL-CAA (181141..181178,181211..181254) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 53.68 Scer_1.trna4S 0 182522 182603 ON 1 tS-AGA complement(182522..182603) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YAR027W 1 183770 184477 ON 1 UIP3 (183770..184477) Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family YAR028W 1 184892 185596 ON 1 YAR028W (184892..185596) Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YAR029W 1 186321 186545 ON 1 YAR029W (186321..186545) Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YAR030C 0 186512 186853 OFF 1 YAR030C complement(186512..186853) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 YAR031W 1 186836 187732 ON 1 PRM9 (186836..187732) Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family YAR033W 1 188107 188811 ON 1 MST28 (188107..188811) Putative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles YAR035W 1 190193 192256 ON 1 YAT1 (190193..192256) Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated YAR035C-A 0 192337 192417 OFF 1 YAR035C-A complement(192337..192417) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational "guilt by association" analysis YAR042W 1 192619 196185 ON 1 SWH1 (192619..196185) Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction YAR047C 0 201467 201787 OFF 1 YAR047C complement(201467..201787) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR050W 1 203403 208016 ON 1 FLO1 (203403..208016) Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p YAR053W 1 208367 208663 OFF 1 YAR053W (208367..208663) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR060C 0 217157 217492 OFF 1 YAR060C complement(217157..217492) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR061W 1 218140 218343 ON 1 YAR061W (218140..218343) Pseudogene with similarity to Flo1p, which is a lectin-like protein involved in flocculation YAR062W 1 218549 219145 OFF 1 YAR062W (218549..219145) Pseudogene with similarity to Flo1p, which is a lectin-like protein involved in flocculation YAR064W 1 220198 220497 ON 1 YAR064W (220198..220497) Putative protein of unknown function YAR066W 1 221049 221660 ON 1 YAR066W (221049..221660) Putative GPI protein YAR068W 1 222406 222891 ON 1 YAR068W (222406..222891) Fungal-specific protein of unknown function; induced in respiratory-deficient cells YAR069C 0 224011 224304 OFF 1 YAR069C complement(224011..224304) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR070C 0 224563 224862 OFF 1 YAR070C complement(224563..224862) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR071W 1 225460 226863 ON 1 PHO11 (225460..226863) One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 YAR073W 1 227742 228953 ON 1 IMD1 (227742..228953) Nonfunctional protein with homology to IMP dehydrogenase; probable pseudogene, located close to the telomere; is not expressed at detectable levels; YAR073W and YAR075W comprise a continuous reading frame in some strains of S. cerevisiae YAR075W 1 228844 229317 ON 1 YAR075W (228844..229317) Non-functional protein with homology IMP dehydrogenase; YAR073W/IMD1 and YAR075W comprise a continuous reading frame in some strains of S. cerevisiae. YBL113C 0 280 2658 ON 2 YBL113C complement(280..2658) Helicase-like protein encoded within the telomeric Y' element YBL113W-A 1 646 1128 OFF 2 YBL113W-A (646..1128) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBL112C 0 2582 2899 ON 2 YBL112C complement(2582..2899) Putative protein of unknown function; YBL112C is contained within TEL02L YBL111C 0 2907 5009 ON 2 YBL111C complement(2907..4116,4216..5009) Helicase-like protein encoded within the telomeric Y' element YBL109W 1 5790 6125 OFF 2 YBL109W (5790..6125) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBL108C-A 0 7605 7733 ON 2 PAU9 complement(7605..7733) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YBL108W 1 8177 8482 OFF 2 YBL108W (8177..8482) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL107W-A 1 9268 9372 OFF 2 YBL107W-A (9268..9372) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the Ty2 LTR YBLWdelta2 Scer_2.trna1L 1 9583 9666 ON 2 tL-UAA (9583..9666) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YBL107C 0 9961 10551 ON 2 YBL107C complement(9961..10551) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBL107C is not an essential gene YBL106C 0 10847 13879 ON 2 SRO77 complement(10847..13879) Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p YBL105C 0 14241 17696 ON 2 PKC1 complement(14241..17696) Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) YBL104C 0 18177 21293 ON 2 SEA4 complement(18177..21293) Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites YBL103C 0 22076 23536 ON 2 RTG3 complement(22076..23536) Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways YBL102W 1 24098 24745 ON 2 SFT2 (24098..24745) Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 YBL101C 0 24946 28299 ON 2 ECM21 complement(24946..28299) Protein involved in regulating the endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements YBL100W-C 1 28427 28546 OFF 2 YBL100W-C (28427..28546) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Scer_2.trna2F 1 36398 36488 ON 2 tF-GAA (36398..36434,36453..36488) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 68.89 YBL100C 0 36989 37303 OFF 2 YBL100C complement(36989..37303) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1 YBL099W 1 37053 38690 ON 2 ATP1 (37053..38690) Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated YBL098W 1 39145 40527 ON 2 BNA4 (39145..40527) Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease YBL097W 1 40831 43095 ON 2 BRN1 (40831..43095) Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission YBL096C 0 43174 43482 OFF 2 YBL096C complement(43174..43482) Non-essential protein of unknown function YBL095W 1 43277 44089 ON 2 YBL095W (43277..44089) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBL094C 0 43765 44097 OFF 2 YBL094C complement(43765..44097) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W YBL093C 0 44256 44918 ON 2 ROX3 complement(44256..44918) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme YBL092W 1 45978 46370 ON 2 RPL32 (45978..46370) Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencing YBL091C-A 0 46565 47180 ON 2 SCS22 complement(46565..47058,47147..47180) Protein involved in regulation of phospholipid metabolism; homolog of Scs2p; similar to D. melanogaster inturned protein YBL091C 0 47363 48628 ON 2 MAP2 complement(47363..48628) Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p YBL090W 1 48825 49358 ON 2 MRP21 (48825..49358) Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences YBL089W 1 49574 50953 ON 2 AVT5 (49574..50953) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YBL088C 0 51019 59382 ON 2 TEL1 complement(51019..59382) Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) YBL087C 0 59822 60739 ON 2 RPL23A complement(59822..60193,60698..60739) Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli L14 and rat L23 ribosomal proteins YBL086C 0 61202 62602 ON 2 YBL086C complement(61202..62602) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YBL085W 1 63876 66818 ON 2 BOI1 (63876..66818) Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain YBL084C 0 67169 69445 ON 2 CDC27 complement(67169..69445) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YBL083C 0 69713 70138 OFF 2 YBL083C complement(69713..70138) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 YBL082C 0 69751 71127 ON 2 ALG3 complement(69751..71127) Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins YBL081W 1 71866 72972 ON 2 YBL081W (71866..72972) Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport YBL080C 0 73070 74695 ON 2 PET112 complement(73070..74695) Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog YBL079W 1 75259 79767 ON 2 NUP170 (75259..79767) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; has similarity to Nup157p; both Nup170p and NUP157p are similar to human Nup155p YBL078C 0 80378 80731 ON 2 ATG8 complement(80378..80731) Component of autophagosomes and Cvt vesicles; undergoes conjugation to phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation YBL077W 1 80897 81328 OFF 2 YBL077W (80897..81328) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C YBL076C 0 81043 84261 ON 2 ILS1 complement(81043..84261) Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A YBL075C 0 84499 86448 ON 2 SSA3 complement(84499..86448) ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm YBL074C 0 86722 87789 ON 2 AAR2 complement(86722..87789) Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron YBL073W 1 87646 87957 OFF 2 YBL073W (87646..87957) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Scer_snR56 1 88190 88277 ON 2 SNR56 (88190..88277) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position G1428 YBL072C 0 88523 89125 ON 2 RPS8A complement(88523..89125) Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein YBL071C-B 0 89458 89556 OFF 2 YBL071C-B complement(89458..89556) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBL071W-A 1 89978 90226 ON 2 KTI11 (89978..90226) Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs YBL071C 0 90223 90531 OFF 2 YBL071C complement(90223..90531) Dubious open reading frame, predicted protein contains a peroxisomal targeting signal YBL070C 0 90605 90925 OFF 2 YBL070C complement(90605..90925) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL069W 1 90741 92030 ON 2 AST1 (90741..92030) Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily YBL068W-A 1 91794 92030 OFF 2 YBL068W-A (91794..92030) Dubious open reading frame unlikely to encode a protein; identified by fungal homology and RT-PCR YBL068W 1 92414 93394 ON 2 PRS4 (92414..93394) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YBL067C 0 93639 95882 ON 2 UBP13 complement(93639..95882) Putative ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions YBL066C 0 96669 100115 ON 2 SEF1 complement(96669..100115) Putative transcription factor, has homolog in Kluyveromyces lactis YBL065W 1 99963 100307 OFF 2 YBL065W (99963..100307) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene YBL064C 0 100371 101156 ON 2 PRX1 complement(100371..101156) Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated YBL063W 1 101886 105221 ON 2 KIP1 (101886..105221) Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions YBL062W 1 105308 105688 OFF 2 YBL062W (105308..105688) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL061C 0 105316 107406 ON 2 SKT5 complement(105316..107406) Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation YBL060W 1 107932 109995 ON 2 YEL1 (107932..109995) Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip YBL059C-A 0 110125 110539 ON 2 CMC2 complement(110125..110420,110506..110539) Protein of the mitochondrial intermembrane space with a role in respiratory chain complex assembly or maintenance; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold YBL059W 1 110594 111244 ON 2 YBL059W (110594..110879,110949..111244) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBL058W 1 111437 112708 ON 2 SHP1 (111437..112708) UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate YBL057C 0 112801 113427 ON 2 PTH2 complement(112801..113427) One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth YBL056W 1 113762 115168 ON 2 PTC3 (113762..115168) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation YBL055C 0 115573 116829 ON 2 YBL055C complement(115573..116829) 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases YBL054W 1 117589 119166 ON 2 TOD6 (117589..119166) PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor, similar to Dot6p; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manne YBL053W 1 119335 119709 OFF 2 YBL053W (119335..119709) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL052C 0 119379 121874 ON 2 SAS3 complement(119379..121874) Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal YBL051C 0 122753 124759 ON 2 PIN4 complement(122753..124759) Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage YBL050W 1 125125 126119 ON 2 SEC17 (125125..125154,125271..126119) Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP YBL049W 1 126828 127244 ON 2 MOH1 (126828..127244) Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase YBL048W 1 127299 127610 OFF 2 RRT1 (127299..127610) Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBL047C 0 127895 132040 ON 2 EDE1 complement(127895..132040) Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death YBL046W 1 132424 133749 ON 2 PSY4 (132424..133749) Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 YBL045C 0 134143 135516 ON 2 COR1 complement(134143..135516) Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain YBL044W 1 135998 136366 OFF 2 YBL044W (135998..136366) Putative protein of unknown function; YBL044W is not an essential protein YBL043W 1 136688 137461 ON 2 ECM13 (136688..137461) Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation YBL042C 0 138341 140260 ON 2 FUI1 complement(138341..140260) High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport YBL041W 1 141247 141972 ON 2 PRE7 (141247..141972) Beta 6 subunit of the 20S proteasome YBL040C 0 142112 142868 ON 2 ERD2 complement(142112..142749,142847..142868) HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins YBL039W-B 1 143393 143572 ON 2 YBL039W-B (143393..143572) Putative protein of unknown function YBL039C 0 143989 145728 ON 2 URA7 complement(143989..145728) Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis YBL039C-A 0 144948 145031 OFF 2 YBL039C-A complement(144948..145031) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF URA7; identified by expression profiling and mass spectrometry YBL038W 1 146187 146885 ON 2 MRPL16 (146187..146885) Mitochondrial ribosomal protein of the large subunit YBL037W 1 147209 150286 ON 2 APL3 (147209..150286) Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport YBL036C 0 150447 151220 ON 2 YBL036C complement(150447..151220) Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS YBL035C 0 151493 153610 ON 2 POL12 complement(151493..153610) B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation YBL034C 0 153848 158389 ON 2 STU1 complement(153848..158389) Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles YBL033C 0 158656 159693 ON 2 RIB1 complement(158656..159693) GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway YBL032W 1 160184 161329 ON 2 HEK2 (160184..161329) RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K YBL031W 1 161699 162715 ON 2 SHE1 (161699..162715) Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function YBL030C 0 163041 163997 ON 2 PET9 complement(163041..163997) Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation YBL029C-A 0 164488 164772 ON 2 YBL029C-A complement(164488..164772) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica YBL029W 1 166134 167264 ON 2 YBL029W (166134..167264) Non-essential protein of unknown function YBL028C 0 167518 167838 ON 2 YBL028C complement(167518..167838) Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis YBL027W 1 168423 169376 ON 2 RPL19B (168423..168424,168809..169376) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal Scer_YGOB_YBL026W-A 0 169634 169870 ON 2 YBL026W-A complement(169634..169870) ANNOTATED BY YGOB - similarity to SAKL0B06622g YBL026W 1 170623 171038 ON 2 LSM2 (170623..170676,170805..171038) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YBL025W 1 171481 171918 ON 2 RRN10 (171481..171918) Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I YBL024W 1 172534 174588 ON 2 NCL1 (172534..174588) S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 YBL023C 0 174920 177526 ON 2 MCM2 complement(174920..177526) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex YBL022C 0 177874 181275 ON 2 PIM1 complement(177874..181275) ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria YBL021C 0 181660 182094 ON 2 HAP3 complement(181660..182094) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding YBL020W 1 182401 184125 ON 2 RFT1 (182401..184125) Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein YBL019W 1 184353 185915 ON 2 APN2 (184353..185915) Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII YBL018C 0 185998 186474 ON 2 POP8 complement(185998..186352,186428..186474) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YBL017C 0 186844 191583 ON 2 PEP1 complement(186844..191583) Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments YBL016W 1 192451 193512 ON 2 FUS3 (192451..193512) Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its degradation YBL015W 1 194122 195702 ON 2 ACH1 (194122..195702) Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Scer_2.trna3I 1 197494 197567 ON 2 tI-AAU (197494..197567) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 Scer_2.trna4G 0 197629 197699 ON 2 tG-GCC complement(197629..197699) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YBL014C 0 199064 201748 ON 2 RRN6 complement(199064..201748) Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p YBL013W 1 202056 203261 ON 2 FMT1 (202056..203261) Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate YBL012C 0 203406 203807 OFF 2 YBL012C complement(203406..203807) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL011W 1 203538 205817 ON 2 SCT1 (203538..205817) Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed YBL010C 0 206107 206949 ON 2 YBL010C complement(206107..206949) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles YBL009W 1 207194 209224 ON 2 ALK2 (207194..209224) Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk1p and to mammalian haspins YBL008W-A 1 209409 209648 OFF 2 YBL008W-A (209409..209648) Putative protein of unknown function; identified by fungal homology and RT-PCR YBL008W 1 209653 212175 ON 2 HIR1 (209653..212175) Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores YBL007C 0 212632 216366 ON 2 SLA1 complement(212632..216366) Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains YBL006C 0 216587 217129 ON 2 LDB7 complement(216587..217129) Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions YBL006W-A 1 216711 216860 OFF 2 YBL006W-A (216711..216860) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene LDB7/YBL006C YBL005W 1 217470 220400 ON 2 PDR3 (217470..220400) Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome Scer_2.trna5S 1 227075 227156 ON 2 tS-AGA (227075..227156) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YBL004W 1 227636 235117 ON 2 UTP20 (227636..235117) Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA YBL003C 0 235394 235792 ON 2 HTA2 complement(235394..235792) Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p YBL002W 1 236492 236887 ON 2 HTB2 (236492..236887) Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation YBL001C 0 237153 237467 ON 2 ECM15 complement(237153..237467) Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis Scer_CEN2 1 238207 238323 ON 2 CEN2 (238207..238323) Chromosome II centromere YBR001C 0 238941 241283 ON 2 NTH2 complement(238941..241283) Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses YBR002C 0 241708 242568 ON 2 RER2 complement(241708..242568) Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting YBR003W 1 242809 244230 ON 2 COQ1 (242809..244230) Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis YBR004C 0 244366 245667 ON 2 GPI18 complement(244366..245667) Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria YBR005W 1 245906 246547 ON 2 RCR1 (245906..246547) Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane YBR006W 1 247010 248503 ON 2 UGA2 (247010..248503) Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm YBR007C 0 248806 251016 ON 2 DSF2 complement(248806..251016) Deletion suppressor of mpt5 mutation YBR008C 0 252563 254209 ON 2 FLR1 complement(252563..254209) Plasma membrane multidrug transporter of the major facilitator superfamily, involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin YBR009C 0 255373 255684 ON 2 HHF1 complement(255373..255684) Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity YBR010W 1 256331 256741 ON 2 HHT1 (256331..256741) Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation YBR011C 0 257112 257975 ON 2 IPP1 complement(257112..257975) Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Scer_YGOB_YBR013C-YBR012C 0 259147 265881 ON 2 YBR013C-YBR012C complement(259147..259577,265500..265881) ANNOTATED BY YGOB - Gene is interrupted by a Ty element in S288c but not YJM789. YBR012C 0 259147 259566 OFF 2 YBR012C complement(259147..259566) Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p YBR013C 0 265492 265881 OFF 2 YBR013C complement(265492..265881) Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Scer_2.trna6T 1 266378 266450 ON 2 tT-AGU (266378..266450) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YBR014C 0 266725 267336 ON 2 GRX7 complement(266725..267336) Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; functional overlap with GRX6 YBR015C 0 267710 269503 ON 2 MNN2 complement(267710..269503) Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment YBR016W 1 270247 270633 ON 2 YBR016W (270247..270633) Tail-anchored plasma membrane protein containing a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions YBR017C 0 270947 273703 ON 2 KAP104 complement(270947..273703) Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis YBR018C 0 274427 275527 ON 2 GAL7 complement(274427..275527) Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism YBR019C 0 276253 278352 ON 2 GAL10 complement(276253..278352) UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers YBR020W 1 279021 280607 ON 2 GAL1 (279021..280607) Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p YBR021W 1 281443 283344 ON 2 FUR4 (281443..283344) Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues YBR022W 1 283738 284271 ON 2 POA1 (283738..284271) Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing YBR023C 0 284428 287925 ON 2 CHS3 complement(284428..287925) Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan YBR024W 1 289445 290350 ON 2 SCO2 (289445..290350) Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p YBR025C 0 290681 291865 ON 2 OLA1 complement(290681..291865) P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein levels are induced by hydrogen peroxide YBR026C 0 292877 294019 ON 2 ETR1 complement(292877..294019) 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis YBR027C 0 294024 294356 OFF 2 YBR027C complement(294024..294356) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR028C 0 294425 296002 ON 2 YPK3 complement(294425..296002) An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner YBR029C 0 296369 297742 ON 2 CDS1 complement(296369..297742) Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids YBR030W 1 298292 299950 ON 2 RKM3 (298292..299950) Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein YBR031W 1 300166 301254 ON 2 RPL4A (300166..301254) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins YBR032W 1 301519 301821 OFF 2 YBR032W (301519..301821) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBR033W 1 301944 304703 ON 2 EDS1 (301944..304703) Putative zinc cluster protein, predicted to be a transcription factor; YBR033W is not an essential gene YBR034C 0 304930 305976 ON 2 HMT1 complement(304930..305976) Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p YBR035C 0 306269 306955 ON 2 PDX3 complement(306269..306955) Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Scer_snR161 0 307185 307345 ON 2 SNR161 complement(307185..307345) H/ACA small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at positions U632 and U766 Scer_TLC1 1 307587 308887 ON 2 TLC1 (307587..308887) TLC1 RNA contains a template sequence that Est2p uses to add irregular repeats of TG(1-3) residues onto a DNA end; RNA template component of telomerase YBR036C 0 309081 310313 ON 2 CSG2 complement(309081..310313) Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations YBR037C 0 310565 311452 ON 2 SCO1 complement(310565..311452) Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins YBR038W 1 311898 314789 ON 2 CHS2 (311898..314789) Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis YBR039W 1 315575 316510 ON 2 ATP3 (315575..316510) Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YBR040W 1 316968 317864 ON 2 FIG1 (316968..317864) Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating YBR041W 1 318266 320275 ON 2 FAT1 (318266..320275) Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids YBR042C 0 320416 321609 ON 2 CST26 complement(320416..321609) Protein required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed YBR043C 0 321876 323945 ON 2 QDR3 complement(321876..323945) Multidrug transporter of the major facilitator superfamily, has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin YBR044C 0 324341 326059 ON 2 TCM62 complement(324341..326059) Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Scer_2.trna7V 0 326792 326865 ON 2 tV-UAC complement(326792..326865) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: UAC | Cove Score: 74.06 YBR045C 0 328173 330092 ON 2 GIP1 complement(328173..330092) Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p YBR046C 0 330507 331511 ON 2 ZTA1 complement(330507..331511) NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin YBR047W 1 331833 332360 ON 2 FMP23 (331833..332360) Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR048W 1 332831 333812 ON 2 RPS11B (332831..332875,333387..333812) Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins YBR049C 0 334386 336818 ON 2 REB1 complement(334386..336818) RNA polymerase I enhancer binding protein; DNA binding protein which binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription YBR050C 0 337183 338199 ON 2 REG2 complement(337183..338199) Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease YBR051W 1 337988 338338 OFF 2 YBR051W (337988..338338) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase YBR052C 0 338720 339352 ON 2 RFS1 complement(338720..339352) Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YBR053C 0 339675 340751 ON 2 YBR053C complement(339675..340751) Putative protein of unknown function; induced by cell wall perturbation YBR054W 1 343101 344135 ON 2 YRO2 (343101..344135) Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p YBR055C 0 344602 347301 ON 2 PRP6 complement(344602..347301) Splicing factor, component of the U4/U6-U5 snRNP complex Scer_2.trna8L 1 347603 347686 ON 2 tL-UAA (347603..347686) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YBR056W 1 347879 349384 ON 2 YBR056W (347879..349384) Putative cytoplasmic protein of unknown function Scer_2.trna9Q 1 350827 350898 ON 2 tQ-UUG (350827..350898) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 65.72 YBR056W-A 1 351255 351455 ON 2 YBR056W-A (351255..351455) Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B YBR056C-B 0 351293 351451 OFF 2 YBR056C-B complement(351293..351451) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the dubious ORF YBR056W-A YBR057C 0 352193 353293 ON 2 MUM2 complement(352193..353293) Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex YBR058C 0 353672 356017 ON 2 UBP14 complement(353672..356017) Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T YBR058C-A 0 356324 356566 ON 2 TSC3 complement(356324..356566) Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis YBR059C 0 356861 360187 ON 2 AKL1 complement(356861..360187) Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization YBR060C 0 360652 362514 ON 2 ORC2 complement(360652..362514) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p YBR061C 0 364787 365719 ON 2 TRM7 complement(364787..365719) 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop YBR062C 0 365976 366600 ON 2 YBR062C complement(365976..366502,366585..366600) Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. YBR063C 0 366970 368184 ON 2 YBR063C complement(366970..368184) Putative protein of unknown function; YBR063C is not an essential gene YBR064W 1 367763 368191 OFF 2 YBR064W (367763..368191) Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C YBR065C 0 368584 369678 ON 2 ECM2 complement(368584..369678) Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p YBR066C 0 370037 370699 ON 2 NRG2 complement(370037..370699) Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p YBR067C 0 372103 372735 ON 2 TIP1 complement(372103..372735) Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins YBR068C 0 373861 375690 ON 2 BAP2 complement(373861..375690) High-affinity leucine permease, functions as a branched-chain amino acid permease involved in the uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains YBR069C 0 376574 378433 ON 2 TAT1 complement(376574..378433) Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance YBR070C 0 379221 379934 ON 2 ALG14 complement(379221..379934) Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases YBR071W 1 380411 381046 ON 2 YBR071W (380411..381046) Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking YBR072W 1 382030 382674 ON 2 HSP26 (382030..382674) Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity YBR072C-A 0 382860 383021 OFF 2 YBR072C-A complement(382860..383021) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR073W 1 383112 385988 ON 2 RDH54 (383112..385988) DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p YBR074W 1 386286 389216 ON 2 YBR074W (386286..389216) Putative metalloprotease YBR076W 1 390227 391291 ON 2 ECM8 (390227..391291) Non-essential protein of unknown function YBR076C-A 0 391351 391617 OFF 2 YBR076C-A complement(391351..391617) Dubious open reading frame unlikely to encode a protein; partially overlaps verified gene ECM8; identified by fungal homology and RT-PCR YBR077C 0 391805 392293 ON 2 SLM4 complement(391805..392293) Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 YBR078W 1 393123 394742 ON 2 ECM33 (393123..393180,393511..394742) GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p YBR079C 0 395383 398277 ON 2 RPG1 complement(395383..398277) eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation YBR080C 0 398614 400890 ON 2 SEC18 complement(398614..400890) ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF YBR081C 0 401253 405251 ON 2 SPT7 complement(401253..405251) Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Scer_2.trna10R 1 405878 405949 ON 2 tR-UCU (405878..405949) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA); decodes AGA codons into arginine; one of 19 nuclear tRNAs for arginine; part of dicistronic transcript with tD(GUC)B; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 Scer_2.trna11D 1 405960 406031 ON 2 tD-GUC (405960..406031) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YBR082C 0 406628 407169 ON 2 UBC4 complement(406628..407027,407123..407169) Ubiquitin-conjugating enzyme (E2), mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response YBR083W 1 409169 410629 ON 2 TEC1 (409169..410629) Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member YBR084W 1 411054 413981 ON 2 MIS1 (411054..413981) Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase YBR084C-A 0 414186 415261 ON 2 RPL19A complement(414186..414753,415260..415261) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal YBR085W 1 415983 416906 ON 2 AAC3 (415983..416906) Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration YBR085C-A 0 418907 419164 ON 2 YBR085C-A complement(418907..419164) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus YBR086C 0 420201 423041 ON 2 IST2 complement(420201..423041) Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process YBR087W 1 423765 424829 ON 2 RFC5 (423765..424829) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YBR088C 0 424990 425766 ON 2 POL30 complement(424990..425766) Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair YBR089W 1 425183 425782 OFF 2 YBR089W (425183..425782) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 YBR089C-A 0 426190 426489 ON 2 NHP6B complement(426190..426489) High-mobility group (HMG) protein that binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2 YBR090C 0 426333 427058 OFF 2 YBR090C complement(426333..426516,426874..427058) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YBR091C 0 427155 427484 ON 2 TIM12 complement(427155..427484) Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins YBR092C 0 427698 429101 ON 2 PHO3 complement(427698..429101) Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin YBR093C 0 429548 430951 ON 2 PHO5 complement(429548..430951) Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 YBR094W 1 432036 434297 ON 2 PBY1 (432036..434297) Putative tubulin tyrosine ligase associated with P-bodies YBR095C 0 434406 435698 ON 2 RXT2 complement(434406..435698) Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth YBR096W 1 436021 436713 ON 2 YBR096W (436021..436713) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER YBR097W 1 436951 441315 ON 2 VPS15 (436951..441315) Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p YBR098W 1 441515 443590 ON 2 MMS4 (441515..443590) Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p YBR099C 0 442924 443307 OFF 2 YBR099C complement(442924..443307) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 YBR101C 0 443821 444693 ON 2 FES1 complement(443821..444693) Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum YBR102C 0 445062 447323 ON 2 EXO84 complement(445062..447323) Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis YBR103W 1 447709 449316 ON 2 SIF2 (447709..449316) WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus YBR103C-A 0 449320 449463 OFF 2 YBR103C-A complement(449320..449463) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; identified by homology to related yeast YBR104W 1 449667 450656 ON 2 YMC2 (449667..450656) Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc1p YBR105C 0 450881 451969 ON 2 VID24 complement(450881..451969) Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase YBR106W 1 452658 453224 ON 2 PHO88 (452658..453224) Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations YBR107C 0 453793 454530 ON 2 IML3 complement(453793..454530) Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p YBR108W 1 454822 457665 ON 2 AIM3 (454822..457665) Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss YBR109C 0 457919 458362 ON 2 CMD1 complement(457919..458362) Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin YBR109W-A 1 458608 458832 OFF 2 YBR109W-A (458608..458832) Putative protein of unknown function; questionable ORF from MIPS YBR110W 1 458872 460221 ON 2 ALG1 (458872..460221) Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog YBR111C 0 461178 461873 ON 2 YSA1 complement(461178..461873) Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate YBR111W-A 1 462139 462579 ON 2 SUS1 (462139..462209,462290..462429,462500..462579) Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP YBR112C 0 462870 465770 ON 2 CYC8 complement(462870..465770) General transcriptional co-repressor, acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+] YBR113W 1 465566 466048 OFF 2 YBR113W (465566..466048) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 YBR114W 1 467248 469620 ON 2 RAD16 (467248..469620) Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex YBR115C 0 469748 473926 ON 2 LYS2 complement(469748..473926) Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p YBR116C 0 474199 474726 OFF 2 YBR116C complement(474199..474726) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 YBR117C 0 474392 476437 ON 2 TKL2 complement(474392..476437) Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids YBR118W 1 477671 479047 ON 2 TEF2 (477671..479047) Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus YBR119W 1 479338 480323 ON 2 MUD1 (479338..479345,479435..480323) U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing YBR120C 0 480435 480923 ON 2 CBP6 complement(480435..480923) Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex YBR121C 0 481364 483367 ON 2 GRS1 complement(481364..483367) Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation YBR121C-A 0 482326 482484 OFF 2 YBR121C-A complement(482326..482484) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely contianed within the verified gene GRS1; identified by expression profiling and mass spectrometry YBR122C 0 483970 484503 ON 2 MRPL36 complement(483970..484503) Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region YBR123C 0 484742 486691 ON 2 TFC1 complement(484742..486691) One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 YBR124W 1 486507 486866 OFF 2 YBR124W (486507..486866) Putative protein of unknown function YBR125C 0 487199 488380 ON 2 PTC4 complement(487199..488380) Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity YBR126C 0 488905 490392 ON 2 TPS1 complement(488905..490392) Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway YBR126W-A 1 490850 491056 OFF 2 YBR126W-A (490850..491056) Protein of unknown function; identified by gene-trapping, microarray analysis, and genome-wide homology searches; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR126W-B YBR126W-B 1 490927 491070 OFF 2 YBR126W-B (490927..491070) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YBR126W-A; Identified by SAGE YBR127C 0 491269 492822 ON 2 VMA2 complement(491269..492822) Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm YBR128C 0 493081 494115 ON 2 ATG14 complement(493081..494115) Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; homolog of human Barkor YBR129C 0 494353 495339 ON 2 OPY1 complement(494353..495339) Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR130C 0 495592 496869 ON 2 SHE3 complement(495592..496869) Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance YBR131W 1 497163 499277 ON 2 CCZ1 (497163..499277) Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway YBR131C-A 0 497650 497739 OFF 2 YBR131C-A complement(497650..497739) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR131W. Identified by gene-trapping, microarray analysis, and genome-wide homology searches YBR132C 0 499652 501442 ON 2 AGP2 complement(499652..501442) High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease YBR133C 0 501804 504287 ON 2 HSL7 complement(501804..504287) Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p YBR134W 1 504243 504644 OFF 2 YBR134W (504243..504644) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YBR135W 1 504854 505306 ON 2 CKS1 (504854..505306) Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters YBR136W 1 505668 512774 ON 2 MEC1 (505668..512774) Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres YBR137W 1 513044 513583 ON 2 YBR137W (513044..513583) Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene YBR138C 0 513762 515336 ON 2 YBR138C complement(513762..515336) Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene YBR139W 1 515664 517190 ON 2 YBR139W (515664..517190) Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner YBR140C 0 517350 526628 ON 2 IRA1 complement(517350..526628) GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase YBR141C 0 527025 528038 ON 2 YBR141C complement(527025..528038) Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene YBR141W-A 1 527167 527262 OFF 2 YBR141W-A (527167..527262) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YBR141C; identified by gene-trapping, microarray analysis, and genome-wide homology searching YBR142W 1 528317 530638 ON 2 MAK5 (528317..530638) Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits YBR143C 0 530869 532182 ON 2 SUP45 complement(530869..532182) Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin YBR144C 0 533235 533549 OFF 2 YBR144C complement(533235..533549) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene YBR145W 1 533762 534817 ON 2 ADH5 (533762..534817) Alcohol dehydrogenase isoenzyme V; involved in ethanol production YBR146W 1 535260 536096 ON 2 MRPS9 (535260..536096) Mitochondrial ribosomal protein of the small subunit YBR147W 1 536575 537465 ON 2 RTC2 (536575..537465) Protein of unknown function; mutant produces large lipid droplets, is resistant to fluconazole, has decreased levels of rDNA transcription, growth defects on minimal media, and suppresses cdc13-1; detected in highly purified mitochondria; similar to a G-protein coupled receptor from S. pombe YBR148W 1 537876 539705 ON 2 YSW1 (537876..539705) Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane YBR149W 1 539987 541021 ON 2 ARA1 (539987..541021) NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product YBR150C 0 541209 544493 ON 2 TBS1 complement(541209..544493) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR151W 1 545028 545978 ON 2 APD1 (545028..545978) Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus YBR152W 1 546376 547251 ON 2 SPP381 (546376..547251) mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p YBR153W 1 547460 548194 ON 2 RIB7 (547460..548194) Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway YBR154C 0 548362 549009 ON 2 RPB5 complement(548362..549009) RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation YBR155W 1 549771 550928 ON 2 CNS1 (549771..550928) TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion YBR156C 0 551104 553200 ON 2 SLI15 complement(551104..553200) Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly YBR157C 0 553543 554310 ON 2 ICS2 complement(553543..554310) Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization YBR158W 1 556549 558198 ON 2 AMN1 (556549..558198) Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) YBR159W 1 558685 559728 ON 2 IFA38 (558685..559728) Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides YBR160W 1 560078 560974 ON 2 CDC28 (560078..560974) Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics YBR161W 1 561634 562764 ON 2 CSH1 (561634..562764) Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p YBR162C 0 563203 564570 ON 2 TOS1 complement(563203..564570) Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C YBR162W-A 1 565231 565428 ON 2 YSY6 (565231..565428) Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion YBR163W 1 565723 567480 ON 2 EXO5 (565723..567480) Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 YBR164C 0 567875 568426 ON 2 ARL1 complement(567875..568426) Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor YBR165W 1 568852 569685 ON 2 UBS1 (568852..569685) Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity YBR166C 0 569842 571200 ON 2 TYR1 complement(569842..571200) Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels YBR167C 0 571468 571890 ON 2 POP7 complement(571468..571890) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YBR168W 1 572371 573612 ON 2 PEX32 (572371..573612) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p YBR169C 0 573915 575996 ON 2 SSE2 complement(573915..575996) Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p YBR170C 0 576344 578086 ON 2 NPL4 complement(576344..578086) Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) YBR171W 1 578364 578984 ON 2 SEC66 (578364..578984) Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YBR172C 0 579150 581372 ON 2 SMY2 complement(579150..581372) Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; has similarity to S. pombe Mpd2 YBR173C 0 581726 582172 ON 2 UMP1 complement(581726..582172) Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly YBR174C 0 582338 582652 OFF 2 YBR174C complement(582338..582652) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective YBR175W 1 582408 583355 ON 2 SWD3 (582408..583355) Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 YBR176W 1 583720 584658 ON 2 ECM31 (583720..584658) Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate YBR177C 0 584807 586162 ON 2 EHT1 complement(584807..586162) Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane YBR178W 1 586071 586445 OFF 2 YBR178W (586071..586445) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C YBR179C 0 586547 589114 ON 2 FZO1 complement(586547..589114) Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system YBR180W 1 589741 591459 ON 2 DTR1 (589741..591459) Putative dityrosine transporter, required for spore wall synthesis; expressed during sporulation; member of the major facilitator superfamily (DHA1 family) of multidrug resistance transporters YBR181C 0 591712 592774 ON 2 RPS6B complement(591712..592416,592769..592774) Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein YBR182C 0 593506 594864 ON 2 SMP1 complement(593506..594864) Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors YBR182C-A 0 595361 595555 OFF 2 YBR182C-A complement(595361..595555) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR183W 1 596115 597065 ON 2 YPC1 (596115..597065) Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance YBR184W 1 597363 598934 ON 2 YBR184W (597363..598934) Putative protein of unknown function; YBR184W is not an essential gene YBR185C 0 599123 599959 ON 2 MBA1 complement(599123..599959) Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane YBR186W 1 600553 602360 ON 2 PCH2 (600553..602103,602217..602360) Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation YBR187W 1 602634 603476 ON 2 GDT1 (602634..603476) Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis YBR188C 0 603685 604107 ON 2 NTC20 complement(603685..604107) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs YBR189W 1 604508 605508 ON 2 RPS9B (604508..604514,604928..605508) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins YBR190W 1 605966 606277 OFF 2 YBR190W (605966..606277) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W YBR191W 1 606270 607140 ON 2 RPL21A (606270..606280,606669..607140) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein YBR191W-A 1 607149 607223 OFF 2 YBR191W-A (607149..607223) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR192W 1 607652 608785 ON 2 RIM2 (607652..608785) Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family YBR193C 0 609082 609753 ON 2 MED8 complement(609082..609753) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YBR194W 1 610038 610409 ON 2 AIM4 (610038..610409) Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress YBR195C 0 610614 611882 ON 2 MSI1 complement(610614..611882) Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase YBR196C 0 612236 613900 ON 2 PGI1 complement(612236..613900) Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation YBR196C-A 0 614024 614173 OFF 2 YBR196C-A complement(614024..614173) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR196C-B 0 614526 614630 OFF 2 YBR196C-B complement(614526..614630) Putative protein of unknown function; identified by expression profiling and mass spectrometry YBR197C 0 615203 615856 ON 2 YBR197C complement(615203..615856) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene YBR198C 0 616127 618523 ON 2 TAF5 complement(616127..618523) Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification YBR199W 1 618909 620303 ON 2 KTR4 (618909..620303) Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YBR200W 1 620872 622527 ON 2 BEM1 (620872..622527) Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p YBR200W-A 1 622983 623147 OFF 2 YBR200W-A (622983..623147) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR201W 1 623576 624211 ON 2 DER1 (623576..624211) Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p YBR201C-A 0 624493 624696 ON 2 YBR201C-A complement(624493..624696) Putative protein of unknown function YBR202W 1 625772 628309 ON 2 MCM7 (625772..628309) Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex YBR203W 1 629168 631942 ON 2 COS111 (629168..631942) Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR204C 0 632254 633381 ON 2 LDH1 complement(632254..633381) Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) YBR205W 1 633622 634836 ON 2 KTR3 (633622..634836) Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles YBR206W 1 634601 634924 OFF 2 YBR206W (634601..634924) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 YBR207W 1 635146 636543 ON 2 FTH1 (635146..636543) Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis YBR208C 0 636703 642210 ON 2 DUR1,2 complement(636703..642210) Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YBR209W 1 642583 642900 OFF 2 YBR209W (642583..642900) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene Scer_2.trna12C 0 643007 643078 ON 2 tC-GCA complement(643007..643078) Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis; tRNA Type: Cys (C) | Anti-Codon: GCA | Cove Score: 75.04 Scer_2.trna13E 1 645167 645238 ON 2 tE-UUC (645167..645238) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YBR210W 1 645550 645978 ON 2 ERV15 (645550..645978) Protein involved in export of proteins from the endoplasmic reticulum, has similarity to Erv14p YBR211C 0 646158 647132 ON 2 AME1 complement(646158..647132) Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance YBR212W 1 647886 649904 ON 2 NGR1 (647886..649904) RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase YBR213W 1 650368 651192 ON 2 MET8 (650368..651192) Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis YBR214W 1 651415 652998 ON 2 SDS24 (651415..652998) One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; may play an indirect role in fluid-phase endocytosis YBR215W 1 653356 655317 ON 2 HPC2 (653356..653368,653453..655317) Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes YBR216C 0 655576 657600 ON 2 YBP1 complement(655576..657600) Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress YBR217W 1 657832 658392 ON 2 ATG12 (657832..658392) Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation YBR218C 0 658707 662249 ON 2 PYC2 complement(658707..662249) Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis YBR219C 0 662499 663303 OFF 2 YBR219C complement(662499..662581,663003..663303) Putative protein of unknown function; YBR219C is not an essential gene YBR220C 0 662995 664677 ON 2 YBR220C complement(662995..664677) Putative protein of unknown function; YBR220C is not an essential gene YBR221C 0 665153 666253 ON 2 PDB1 complement(665153..666253) E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria YBR221W-A 1 666538 666642 OFF 2 YBR221W-A (666538..666642) Putative protein of unknown function; identified by expression profiling and mass spectrometry YBR222C 0 666720 668351 ON 2 PCS60 complement(666720..668351) Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase YBR223C 0 668663 670297 ON 2 TDP1 complement(668663..670297) Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI YBR223W-A 1 668721 668840 OFF 2 YBR223W-A (668721..668840) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps YBR223C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR224W 1 670125 670640 OFF 2 YBR224W (670125..670640) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 YBR225W 1 670627 673329 ON 2 YBR225W (670627..673329) Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components YBR226C 0 673155 673565 OFF 2 YBR226C complement(673155..673565) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W YBR227C 0 673572 675134 ON 2 MCX1 complement(673572..675134) Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins YBR228W 1 675313 676227 ON 2 SLX1 (675313..676227) Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p YBR229C 0 676357 679221 ON 2 ROT2 complement(676357..679221) Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations YBR230C 0 679549 680050 ON 2 OM14 complement(679549..679942,680040..680050) Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron YBR230W-A 1 680362 680562 ON 2 YBR230W-A (680362..680562) Putative protein of unknown function Scer_LSR1 0 680688 681862 ON 2 LSR1 complement(680688..681862) U2 spliceosomal RNA (U2 snRNA), component of the spliceosome; pairs with the branchpoint sequence; functionally equivalent to mammalian U2 snRNA; stress-induced pseudouridylations at positions 56 and 93 may contribute to regulation of splicing YBR231C 0 682179 683090 ON 2 SWC5 complement(682179..683090) Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YBR232C 0 683373 683732 OFF 2 YBR232C complement(683373..683732) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR233W 1 683428 684669 ON 2 PBP2 (683428..684669) RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length YBR233W-A 1 684977 685261 ON 2 DAD3 (684977..685261) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YBR234C 0 685438 686592 ON 2 ARC40 complement(685438..686592) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YBR235W 1 686901 690263 ON 2 YBR235W (686901..690263) Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene YBR236C 0 690383 691693 ON 2 ABD1 complement(690383..691693) Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA YBR237W 1 691969 694518 ON 2 PRP5 (691969..694518) RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA YBR238C 0 695107 697302 ON 2 YBR238C complement(695107..697302) Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span YBR239C 0 698354 699943 ON 2 ERT1 complement(698354..699943) Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition YBR240C 0 700490 701842 ON 2 THI2 complement(700490..701842) Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type YBR241C 0 702589 704055 ON 2 YBR241C complement(702589..704055) Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene YBR242W 1 704670 705386 ON 2 YBR242W (704670..705386) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene YBR243C 0 705447 706793 ON 2 ALG7 complement(705447..706793) UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin YBR244W 1 707528 708016 ON 2 GPX2 (707528..708016) Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress YBR245C 0 708150 711539 ON 2 ISW1 complement(708150..711539) ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p called Isw1a and a complex with Ioc2p and Ioc4p called Isw1b; Isw1a and Isw1b have both partially overlapping and distinct roles with Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation YBR246W 1 711591 712754 ON 2 RRT2 (711591..712754) Protein required for last step of diphthamide biosynthesis; deletion leads to accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription YBR247C 0 713004 714455 ON 2 ENP1 complement(713004..714455) Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus YBR248C 0 714807 716465 ON 2 HIS7 complement(714807..716465) Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor YBR249C 0 716882 717994 ON 2 ARO4 complement(716882..717994) 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan YBR250W 1 719033 720604 ON 2 SPO23 (719033..720604) Protein of unknown function; associates with meiosis-specific protein Spo1p YBR251W 1 721390 722313 ON 2 MRPS5 (721390..722313) Mitochondrial ribosomal protein of the small subunit YBR252W 1 722611 723054 ON 2 DUT1 (722611..723054) deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate YBR253W 1 723270 723635 ON 2 SRB6 (723270..723635) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YBR254C 0 723736 724263 ON 2 TRS20 complement(723736..724263) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder YBR255W 1 724456 726540 ON 2 MTC4 (724456..726540) Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 YBR255C-A 0 726618 727074 ON 2 YBR255C-A complement(726618..726917,727012..727074) Putative protein of unknown function; may interact with respiratory chain complexes III (ubiquinol-cytochrome c reductase) or IV (cytochrome c oxidase); identified by sequence comparison with hemiascomycetous yeast species YBR256C 0 727386 728102 ON 2 RIB5 complement(727386..728102) Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway YBR257W 1 728885 729724 ON 2 POP4 (728885..729724) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P YBR258C 0 729734 730162 ON 2 SHG1 complement(729734..730162) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres YBR259W 1 730387 732453 ON 2 YBR259W (730387..732453) Putative protein of unknown function; YBR259W is not an essential gene YBR260C 0 732639 734639 ON 2 RGD1 complement(732639..734639) GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization YBR261C 0 734832 735530 ON 2 TAE1 complement(734832..735530) AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm YBR262C 0 735720 736040 ON 2 AIM5 complement(735720..736040) Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture YBR263W 1 736264 737736 ON 2 SHM1 (736264..737736) Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine YBR264C 0 737770 738369 ON 2 YPT10 complement(737770..738369) Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles YBR265W 1 738582 739544 ON 2 TSC10 (738582..739544) 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family YBR267W 1 739841 741022 ON 2 REI1 (739841..741022) Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network YBR266C 0 739936 740388 OFF 2 SLM6 complement(739936..740388) Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase YBR268W 1 741299 741616 ON 2 MRPL37 (741299..741616) Mitochondrial ribosomal protein of the large subunit YBR269C 0 742160 742576 ON 2 FMP21 complement(742160..742576) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR270C 0 742761 744398 ON 2 BIT2 complement(742761..744398) Subunit of TORC2, a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; similar to Bit61p YBR271W 1 744852 746111 ON 2 EFM2 (744852..746111) S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis YBR272C 0 746361 747803 ON 2 HSM3 complement(746361..747803) Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation YBR273C 0 748061 749371 ON 2 UBX7 complement(748061..749371) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p YBR274W 1 749594 751177 ON 2 CHK1 (749594..751177) Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase YBR275C 0 751356 757106 ON 2 RIF1 complement(751356..757106) Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation YBR276C 0 757620 760043 ON 2 PPS1 complement(757620..760043) Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle YBR277C 0 760215 760616 OFF 2 YBR277C complement(760215..760616) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W YBR278W 1 760294 760899 ON 2 DPB3 (760294..760899) Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle YBR279W 1 761257 762594 ON 2 PAF1 (761257..762594) Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 YBR280C 0 762784 764697 ON 2 SAF1 complement(762784..764697) F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 YBR281C 0 764970 767606 ON 2 DUG2 complement(764970..767606) Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) YBR282W 1 768240 768680 ON 2 MRPL27 (768240..768680) Mitochondrial ribosomal protein of the large subunit YBR283C 0 768943 770415 ON 2 SSH1 complement(768943..770415) Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential YBR284W 1 771239 773632 ON 2 YBR284W (771239..773632) Protein of unknown function; some similarity to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin, and synthetic phenotype with expression of alpha-synuclein; induced in response to hydrostatic pressure; not an essential gene YBR285W 1 773922 774356 ON 2 YBR285W (773922..774356) Putative protein of unknown function; YBR285W is not an essential gene YBR286W 1 774700 776313 ON 2 APE3 (774700..776313) Vacuolar aminopeptidase Y, processed to mature form by Prb1p YBR287W 1 776571 777854 ON 2 YBR287W (776571..777854) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene YBR288C 0 778012 779463 ON 2 APM3 complement(778012..779463) Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway YBR289W 1 779667 782384 ON 2 SNF5 (779667..782384) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p YBR290W 1 782591 783556 ON 2 BSD2 (782591..783556) Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification YBR291C 0 783673 784572 ON 2 CTP1 complement(783673..784572) Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family YBR292C 0 784702 785073 OFF 2 YBR292C complement(784702..785073) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene YBR293W 1 787006 788430 ON 2 VBA2 (787006..788430) Permease of basic amino acids in the vacuolar membrane YBR294W 1 789235 791814 ON 2 SUL1 (789235..791814) High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates YBR295W 1 792849 796499 ON 2 PCA1 (792849..796499) Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function YBR296C 0 796798 798522 ON 2 PHO89 complement(796798..798522) Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p YBR296C-A 0 800123 800242 OFF 2 YBR296C-A complement(800123..800242) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR297W 1 800523 801929 ON 2 MAL33 (800523..801929) MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C YBR298C 0 802631 804475 ON 2 MAL31 complement(802631..804475) Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C YBR298C-A 0 805035 805256 OFF 2 YBR298C-A complement(805035..805256) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR299W 1 805351 807105 ON 2 MAL32 (805351..807105) Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose YBR300C 0 808600 809097 OFF 2 YBR300C complement(808600..809097) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene YBR301W 1 809057 809419 ON 2 PAU24 (809057..809419) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth YBR302C 0 810340 811479 ON 2 COS2 complement(810340..811479) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YCL076W 1 1392 2135 OFF 3 YCL076W (1392..2135) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCL075W 1 2126 2566 OFF 3 YCL075W (2126..2566) Pseudogene: encodes fragment of Ty Pol protein YCL074W 1 2824 3750 OFF 3 YCL074W (2824..3750) Pseudogene: encodes fragment of Ty Pol protein YCL073C 0 6479 8326 ON 3 GEX1 complement(6479..8326) Proton:glutathione antiporter localized to the vacuolar and plasma membranes; imports glutathione from the vacuole and exports it through the plasma membrane; has a role in resistance to oxidative stress and modulation of the PKA pathway YCL069W 1 9706 11082 ON 3 VBA3 (9706..11082) Permease of basic amino acids in the vacuolar membrane YCL068C 0 11503 12285 ON 3 YCL068C complement(11503..12285) Putative protein of unknown function YCL067C 0 12386 13018 ON 3 HMLALPHA2 complement(12386..13018) Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression YCL066W 1 13282 13809 ON 3 HMLALPHA1 (13282..13809) Silenced copy of ALPHA1 at HML, encoding a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression YCL065W 1 13751 14119 OFF 3 YCL065W (13751..14119) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps HMLALPHA1 YCL064C 0 15798 16880 ON 3 CHA1 complement(15798..16880) Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine YCL063W 1 17290 18561 ON 3 VAC17 (17290..18561) Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p YCL061C 0 18816 22106 ON 3 MRC1 complement(18816..22106) S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres YCL059C 0 22429 23379 ON 3 KRR1 complement(22429..23379) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit YCL058C 0 23523 23981 OFF 3 FYV5 complement(23523..23981) Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis YCL058W-A 1 23584 23925 ON 3 ADF1 (23584..23925) Transcriptional repressor encoded by the antisense strand of the FYV5 gene; negatively regulates transcription of FYV5 by binding to the promoter on the sense strand YCL057C-A 0 24032 24325 ON 3 MOS1 complement(24032..24325) Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 YCL057W 1 24768 26906 ON 3 PRD1 (24768..26906) Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins YCL056C 0 26925 27359 ON 3 PEX34 complement(26925..27359) Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation YCL055W 1 27929 28936 ON 3 KAR4 (27929..28936) Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone YCL054W 1 31449 33974 ON 3 SPB1 (31449..33974) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants YCL052C 0 34143 35393 ON 3 PBN1 complement(34143..35393) Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X YCL051W 1 35865 37616 ON 3 LRE1 (35865..37616) Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway YCL050C 0 37836 38801 ON 3 APA1 complement(37836..38801) Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p YCL049C 0 39786 40724 ON 3 YCL049C complement(39786..40724) Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene YCL048W-A 1 41488 41727 ON 3 YCL048W-A (41488..41727) Putative protein of unknown function YCL048W 1 42165 43556 ON 3 SPS22 (42165..43556) Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall YCL047C 0 43661 44437 ON 3 POF1 complement(43661..44437) ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p YCL045C 0 44623 46905 ON 3 EMC1 complement(44623..46905) Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p YCL046W 1 46640 46963 OFF 3 YCL046W (46640..46963) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C YCL044C 0 47111 48364 ON 3 MGR1 complement(47111..48364) Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA YCL043C 0 48653 50221 ON 3 PDI1 complement(48653..50221) Protein disulfide isomerase; multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; forms a complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2 which promotes degradation in the unfolded protein response YCL041C 0 50133 50627 OFF 3 YCL041C complement(50133..50627) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W YCL042W 1 50584 50943 OFF 3 YCL042W (50584..50943) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YCL040W 1 50838 52340 ON 3 GLK1 (50838..52340) Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources YCL039W 1 52645 54882 ON 3 GID7 (52645..54882) Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions YCL038C 0 54941 56527 ON 3 ATG22 complement(54941..56527) Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation YCL037C 0 57374 58678 ON 3 SRO9 complement(57374..58678) Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif YCL036W 1 59026 60726 ON 3 GFD2 (59026..60726) Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation YCL035C 0 60841 61173 ON 3 GRX1 complement(60841..61173) Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage YCL034W 1 61658 62722 ON 3 LSB5 (61658..62722) Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat YCL033C 0 62776 63282 ON 3 MXR2 complement(62776..63282) Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan YCL032W 1 63441 64481 ON 3 STE50 (63441..64481) Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction YCL031C 0 64675 65568 ON 3 RRP7 complement(64675..65568) Essential protein involved in rRNA processing and ribosome biogenesis YCL030C 0 65934 68333 ON 3 HIS4 complement(65934..68333) Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis YCL029C 0 68599 69921 ON 3 BIK1 complement(68599..69921) Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 YCL028W 1 70150 71367 ON 3 RNQ1 (70150..71367) [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate YCL027W 1 71803 73341 ON 3 FUS1 (71803..73341) Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate YCL026C-B 0 73405 73986 ON 3 HBN1 complement(73405..73986) Putative protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion YCL026C-A 0 74704 75285 ON 3 FRM2 complement(74704..75285) Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin YCL025C 0 76018 77919 ON 3 AGP1 complement(76018..77919) Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) YCL023C 0 78949 79296 OFF 3 YCL023C complement(78949..79296) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 YCL024W 1 79162 82275 ON 3 KCC4 (79162..82275) Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p YCL022C 0 81570 82085 OFF 3 YCL022C complement(81570..82085) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Scer_3.trna1E 0 82462 82533 ON 3 tE-UUC complement(82462..82533) Glutamine tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YCL021W-A 1 83620 83997 ON 3 YCL021W-A (83620..83997) Putative protein of unknown function Scer_3.trna2L 1 90859 90972 ON 3 tL-CAA (90859..90896,90929..90972) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YCL018W 1 91324 92418 ON 3 LEU2 (91324..92418) Beta-isopropylmalate dehydrogenase (IMDH), catalyzes the third step in the leucine biosynthesis pathway YCL017C 0 92777 94270 ON 3 NFS1 complement(92777..94270) Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria YCL016C 0 94621 95763 ON 3 DCC1 complement(94621..95763) Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance YCL014W 1 96281 101191 ON 3 BUD3 (96281..101191) Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding YCL012C 0 101317 101788 ON 3 YCL012C complement(101317..101633,101701..101788) Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene YCL011C 0 102075 103358 ON 3 GBP2 complement(102075..103358) Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro YCL010C 0 103571 104350 ON 3 SGF29 complement(103571..104350) Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation YCL009C 0 104619 105548 ON 3 ILV6 complement(104619..105548) Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria YCL008C 0 105696 106853 ON 3 STP22 complement(105696..106853) Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype YCL007C 0 106974 107366 OFF 3 YCL007C complement(106974..107366) Dubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A YCL005W-A 1 107023 107417 ON 3 VMA9 (107023..107033,107111..107191,107288..107417) Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Scer_snR43 0 107504 107712 ON 3 SNR43 complement(107504..107712) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U966 and of 5.8S rRNA at position 73 YCL005W 1 108021 108791 ON 3 LDB16 (108021..108791) Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria YCL004W 1 109105 110670 ON 3 PGS1 (109105..110670) Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis YCL002C 0 110808 111675 ON 3 YCL002C complement(110808..111557,111634..111675) Putative protein of unknown function; YCL002C is not an essential gene YCL001W 1 111916 112482 ON 3 RER1 (111916..112482) Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER YCL001W-A 1 113080 113541 OFF 3 YCL001W-A (113080..113541) Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III YCL001W-B 1 113770 114024 ON 3 YCL001W-B (113770..114024) Putative protein of unknown function; YCL001W-B gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Scer_CEN3 1 114385 114501 ON 3 CEN3 (114385..114501) Chromosome III centromere YCR001W 1 115685 115999 OFF 3 YCR001W (115685..115999) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene YCR002C 0 117380 118348 ON 3 CDC10 complement(117380..118348) Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus contributes septin interaction with phosphatidylinositol-4,5-bisphosphate YCR003W 1 118620 119171 ON 3 MRPL32 (118620..119171) Mitochondrial ribosomal protein of the large subunit YCR004C 0 119575 120318 ON 3 YCP4 complement(119575..120318) Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YCR005C 0 120946 122328 ON 3 CIT2 complement(120946..122328) Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors YCR006C 0 122530 123003 OFF 3 YCR006C complement(122530..123003) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Scer_3.trna3P 0 123577 123648 ON 3 tP-AGG complement(123577..123648) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: AGG | Cove Score: 66.99 YCR007C 0 126011 126730 ON 3 YCR007C complement(126011..126730) Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Scer_3.trna4N 1 127716 127789 ON 3 tN-GUU (127716..127789) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YCR008W 1 128470 130281 ON 3 SAT4 (128470..130281) Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p YCR009C 0 130745 131542 ON 3 RVS161 complement(130745..131542) Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress YCR010C 0 132273 133124 ON 3 ADY2 complement(132273..133124) Acetate transporter required for normal sporulation; phosphorylated in mitochondria YCR011C 0 133724 136873 ON 3 ADP1 complement(133724..136873) Putative ATP-dependent permease of the ABC transporter family of proteins YCR012W 1 137746 138996 ON 3 PGK1 (137746..138996) 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis YCR013C 0 138402 139049 OFF 3 YCR013C complement(138402..139049) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant YCR014C 0 139185 140933 ON 3 POL4 complement(139185..140933) DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta YCR015C 0 141217 142170 ON 3 YCR015C complement(141217..142170) Putative protein of unknown function; YCR015C is not an essential gene Scer_snR33 0 142364 142546 ON 3 SNR33 complement(142364..142546) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1042 Scer_3.trna5G 0 142701 142771 ON 3 tG-GCC complement(142701..142771) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis;can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YCR016W 1 143634 144506 ON 3 YCR016W (143634..144506) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis YCR017C 0 144773 147634 ON 3 CWH43 complement(144773..147634) Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion YCR018C 0 148238 148903 ON 3 SRD1 complement(148238..148903) Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation Scer_3.trna6M 1 149920 149991 ON 3 tM-CAU (149920..149991) Methionine tRNA (tRNA-Met), predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 74.96 Scer_3.trna7K 0 151284 151356 ON 3 tK-CUU complement(151284..151356) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YCR018C-A 0 151608 151862 OFF 3 YCR018C-A complement(151608..151862) Putative protein of unknown function; encoded opposite a Ty1 LTR YCR019W 1 152837 153928 ON 3 MAK32 (152837..153928) Protein necessary for structural stability of L-A double-stranded RNA-containing particles YCR020C 0 154012 154659 ON 3 PET18 complement(154012..154659) Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin YCR020C-A 0 154830 155096 ON 3 MAK31 complement(154830..155096) Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family YCR020W-B 1 155320 155556 ON 3 HTL1 (155320..155556) Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance YCR021C 0 156109 157107 ON 3 HSP30 complement(156109..157107) Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase YCR022C 0 157421 157765 OFF 3 YCR022C complement(157421..157765) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene YCR023C 0 158538 160373 ON 3 YCR023C complement(158538..160373) Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene YCR024C 0 160744 162222 ON 3 SLM5 complement(160744..162222) Mitochondrial asparaginyl-tRNA synthetase YCR024C-B 0 162599 162865 OFF 3 YCR024C-B complement(162599..162865) Putative protein of unknown function; identified by expression profiling and mass spectrometry YCR024C-A 0 162945 163067 ON 3 PMP1 complement(162945..163067) Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines YCR025C 0 163446 163856 OFF 3 YCR025C complement(163446..163856) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene YCR026C 0 164111 166339 ON 3 NPP1 complement(164111..166339) Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation YCR027C 0 167370 167999 ON 3 RHB1 complement(167370..167999) Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Scer_3.trna8Q 0 168301 168372 ON 3 tQ-UUG complement(168301..168372) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YCR028C 0 170886 172424 ON 3 FEN2 complement(170886..172424) Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph YCR028C-A 0 172950 173440 ON 3 RIM1 complement(172950..173115,173199..173440) Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication YCR030C 0 173826 176438 ON 3 SYP1 complement(173826..176438) Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Scer_snR65 1 177183 177282 ON 3 SNR65 (177183..177282) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U2347 YCR031C 0 177500 178220 ON 3 RPS14A complement(177500..177906,178214..178220) Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins Scer_snR189 0 178610 178798 ON 3 SNR189 complement(178610..178798) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2735 and small subunit (SSU) rRNA at position U466 YCR032W 1 179520 186023 ON 3 BPH1 (179520..186023) PProtein homologous to human Chediak-Higashi syndrome and murine Beige proteins, which are implicated in disease syndromes due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting YCR033W 1 186489 190169 ON 3 SNT1 (186489..190169) Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance YCR034W 1 190592 191635 ON 3 FEN1 (190592..191635) Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway YCR035C 0 191834 193018 ON 3 RRP43 complement(191834..193018) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8) YCR036W 1 193297 194298 ON 3 RBK1 (193297..194298) Putative ribokinase YCR037C 0 194414 197185 ON 3 PHO87 complement(194414..197185) Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments YCR038C 0 197621 199549 ON 3 BUD5 complement(197621..199549) GTP/GDP exchange factor for Rsr1p (Bud1p) required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types YCR038W-A 1 199293 199418 OFF 3 YCR038W-A (199293..199418) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene BUD5/YCR038C; identified by homology with hemiascomycetous yeast species YCR039C 0 199546 200178 ON 3 MATALPHA2 complement(199546..200178) Homeobox-domain protein that, with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette YCR040W 1 200442 200969 ON 3 MATALPHA1 (200442..200969) Transcriptional co-activator involved in regulation of mating-type-specific gene expression; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette YCR041W 1 200911 201243 OFF 3 YCR041W (200911..201243) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR042C 0 201174 205397 ON 3 TAF2 complement(201174..205397) TFIID subunit (150 kDa), involved in RNA polymerase II transcription initiation YCR043C 0 206261 206644 ON 3 YCR043C complement(206261..206644) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene YCR044C 0 206877 207950 ON 3 PER1 complement(206877..207950) Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 YCR045C 0 208135 209610 ON 3 RRT12 complement(208135..209610) Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores YCR045W-A 1 208751 209101 OFF 3 YCR045W-A (208751..209101) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with YCR045C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR046C 0 209914 210423 ON 3 IMG1 complement(209914..210423) Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome YCR047C 0 210718 211545 ON 3 BUD23 complement(210718..211545) Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern YCR047W-A 1 211301 211507 OFF 3 YCR047W-A (211301..211507) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with YCR047C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR049C 0 211871 212317 OFF 3 YCR049C complement(211871..212317) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR048W 1 211929 213761 ON 3 ARE1 (211929..213761) Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen YCR050C 0 213464 213772 OFF 3 YCR050C complement(213464..213772) Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein YCR051W 1 214071 214739 ON 3 YCR051W (214071..214739) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene YCR052W 1 214994 216445 ON 3 RSC6 (214994..216445) Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p YCR053W 1 216697 218241 ON 3 THR4 (216697..218241) Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway YCR054C 0 218376 220067 ON 3 CTR86 complement(218376..220067) Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels YCR057C 0 220457 223228 ON 3 PWP2 complement(220457..223228) Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis YCR059C 0 223454 224230 ON 3 YIH1 complement(223454..224230) Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT YCR060W 1 224399 224734 ON 3 TAH1 (224399..224734) Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II YCR061W 1 225563 227458 ON 3 YCR061W (225563..227458) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Scer_3.trna9S 1 227942 228042 ON 3 tS-CGA (227942..227978,227998..228042) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; i6A37 modification is catalyzed by Mod5p; can mutate to suppress amber or ochre nonsense mutations; suppressor mutant alleles are recessive lethal and can only be maintained when an additional wild-type copy of the gene is present, because SUP61 encodes the only tRNA species that can decode UCG codons; tRNA Type: Ser (S) | Anti-Codon: CGA | Cove Score: 75.88 YCR064C 0 228095 228505 OFF 3 YCR064C complement(228095..228505) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 YCR063W 1 228318 228791 ON 3 BUD31 (228318..228791) Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis YCR065W 1 229310 231004 ON 3 HCM1 (229310..231004) Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role YCR066W 1 231500 232963 ON 3 RAD18 (231500..232963) E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair YCR067C 0 233125 236322 ON 3 SED4 complement(233125..236322) Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p YCR068W 1 237214 238776 ON 3 ATG15 (237214..238776) Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway YCR069W 1 239055 240011 ON 3 CPR4 (239055..240011) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway YCR071C 0 240103 240543 ON 3 IMG2 complement(240103..240543) Mitochondrial ribosomal protein of the large subunit YCR072C 0 240805 242352 ON 3 RSA4 complement(240805..242352) WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus YCR073C 0 242588 246583 ON 3 SSK22 complement(242588..246583) MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p YCR073W-A 1 246963 247910 ON 3 SOL2 (246963..247910) Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p YCR075C 0 248033 248815 ON 3 ERS1 complement(248033..248815) Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains YCR075W-A 1 248975 249202 ON 3 YCR075W-A (248975..249202) Putative protein of unknown function; identified by homology to Ashbya gossypii YCR076C 0 249293 250045 ON 3 FUB1 complement(249293..250045) Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene YCR077C 0 250238 252628 ON 3 PAT1 complement(250238..252628) Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p YCR079W 1 252845 254173 ON 3 PTC6 (252845..254173) Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase YCR081W 1 254371 258654 ON 3 SRB8 (254371..258654) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression YCR081C-A 0 258415 258651 OFF 3 YCR081C-A complement(258415..258651) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with SRB8/YCR081W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR082W 1 258883 259269 ON 3 AHC2 (258883..259269) Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex YCR083W 1 259578 259961 ON 3 TRX3 (259578..259961) Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p YCR084C 0 260311 262452 ON 3 TUP1 complement(260311..262452) General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes YCR085W 1 262916 263269 OFF 3 YCR085W (262916..263269) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR086W 1 263392 263964 ON 3 CSM1 (263392..263964) Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation YCR087W 1 263976 264491 OFF 3 YCR087W (263976..264491) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene YCR087C-A 0 264006 264467 ON 3 YCR087C-A complement(264006..264467) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene YCR088W 1 265068 266846 ON 3 ABP1 (265068..266846) Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences YCR089W 1 267434 272263 ON 3 FIG2 (267434..272263) Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating YCR090C 0 272315 272863 ON 3 YCR090C complement(272315..272863) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene YCR091W 1 274404 276566 ON 3 KIN82 (274404..276566) Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p YCR092C 0 276764 279820 ON 3 MSH3 complement(276764..279820) Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability YCR093W 1 280117 286443 ON 3 CDC39 (280117..286443) Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor YCR094W 1 286762 287937 ON 3 CDC50 (286762..287937) Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p YCR095C 0 288170 289258 ON 3 OCA4 complement(288170..289258) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YCR095W-A 1 289639 289797 OFF 3 YCR095W-A (289639..289797) Putative protein of unknown function YCR096C 0 293179 293538 OFF 3 HMRA2 complement(293179..293538) Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system YCR097W 1 293835 294321 ON 3 HMRA1 (293835..293939,293994..294239,294292..294321) Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells YCR097W-A 1 294439 294705 OFF 3 YCR097W-A (294439..294705) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by homology to a hemiascomycetous yeast protein Scer_3.trna10T 1 295484 295556 ON 3 tT-AGU (295484..295556) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YCR098C 0 297049 298605 ON 3 GIT1 complement(297049..298605) Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability YCR099C 0 300832 301299 ON 3 YCR099C complement(300832..301299) Putative protein of unknown function YCR100C 0 301271 302221 ON 3 YCR100C complement(301271..302221) Putative protein of unknown function YCR101C 0 302482 303030 ON 3 YCR101C complement(302482..303030) Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene YCR102C 0 304361 305467 ON 3 YCR102C complement(304361..305467) Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family YCR102W-A 1 306735 306932 OFF 3 YCR102W-A (306735..306932) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR104W 1 307801 308175 ON 3 PAU3 (307801..308175) Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YCR105W 1 309070 310155 ON 3 ADH7 (309070..310155) NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance YCR106W 1 310958 313456 ON 3 RDS1 (310958..313456) Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide YCR107W 1 313890 314981 ON 3 AAD3 (313890..314981) Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YCR108C 0 315997 316188 OFF 3 YCR108C complement(315997..316188) Putative protein of unknown function; identified by fungal homology and RT-PCR YDL248W 1 1802 2953 ON 4 COS7 (1802..2953) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDL247W-A 1 3762 3836 OFF 4 YDL247W-A (3762..3836) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species YDL247W 1 5985 7814 ON 4 MPH2 (5985..7814) Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication YDL246C 0 8683 9756 ON 4 SOR2 complement(8683..9756) Protein of unknown function; protein sequence is 99% identical to the Sor1p sorbitol dehydrogenase; computational analysis of large-scale protein-protein interaction data also suggests a role in fructose or mannose metabolism YDL245C 0 11657 13360 ON 4 HXT15 complement(11657..13360) Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose YDL244W 1 16204 17226 ON 4 THI13 (16204..17226) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YDL243C 0 17577 18566 ON 4 AAD4 complement(17577..18566) Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin YDL242W 1 18959 19312 OFF 4 YDL242W (18959..19312) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDL241W 1 20635 21006 ON 4 YDL241W (20635..21006) Putative protein of unknown function; YDL241W is not an essential gene YDL240C-A 0 22471 22608 OFF 4 YDL240C-A complement(22471..22608) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species YDL240W 1 22823 25876 ON 4 LRG1 (22823..25876) Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis YDL239C 0 26403 28775 ON 4 ADY3 complement(26403..28775) Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p YDL238C 0 28985 30454 ON 4 GUD1 complement(28985..30454) Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures YDL237W 1 30657 31829 ON 4 AIM6 (30657..31829) Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene YDL236W 1 32296 33234 ON 4 PHO13 (32296..33234) Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity YDL235C 0 33415 33918 ON 4 YPD1 complement(33415..33918) Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus YDL234C 0 34237 36477 ON 4 GYP7 complement(34237..36477) GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking YDL233W 1 36797 38173 ON 4 YDL233W (36797..38173) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL233W is not an essential gene YDL232W 1 38487 38597 ON 4 OST4 (38487..38597) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex YDL231C 0 38867 42244 ON 4 BRE4 complement(38867..42244) Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport YDL230W 1 42700 43707 ON 4 PTP1 (42700..43707) Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation YDL229W 1 44065 45906 ON 4 SSB1 (44065..45906) Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p YDL228C 0 45277 45918 OFF 4 YDL228C complement(45277..45918) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene SSB1 YDL227C 0 46271 48031 ON 4 HO complement(46271..48031) Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p YDL226C 0 51115 52173 ON 4 GCS1 complement(51115..52173) ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p YDL225W 1 52445 54100 ON 4 SHS1 (52445..54100) Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; undergoes sumoylation and phosphorylation during mitosis YDL224C 0 54397 56346 ON 4 WHI4 complement(54397..56346) Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division YDL223C 0 57265 60405 ON 4 HBT1 complement(57265..60405) Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis YDL222C 0 60872 61801 ON 4 FMP45 complement(60872..61801) Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C YDL221W 1 62011 62562 OFF 4 YDL221W (62011..62562) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 YDL220C 0 62244 65018 ON 4 CDC13 complement(62244..65018) Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation YDL219W 1 65242 65765 ON 4 DTD1 (65242..65306,65378..65765) D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes YDL218W 1 66493 67446 ON 4 YDL218W (66493..67446) Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin YDL217C 0 67983 68606 ON 4 TIM22 complement(67983..68606) Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported YDL216C 0 68997 70319 ON 4 RRI1 complement(68997..70319) Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling YDL215C 0 70640 73918 ON 4 GDH2 complement(70640..73918) NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels YDL214C 0 74446 76545 ON 4 PRR2 complement(74446..76545) Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance YDL213C 0 77289 77966 ON 4 NOP6 complement(77289..77966) rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes YDL212W 1 78426 79058 ON 4 SHR3 (78426..79058) Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface YDL211C 0 79294 80412 ON 4 YDL211C complement(79294..80412) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Scer_4.trna1G 1 83548 83618 ON 4 tG-GCC (83548..83618) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YDL210W 1 84270 85985 ON 4 UGA4 (84270..85985) Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane YDL209C 0 86207 87226 ON 4 CWC2 complement(86207..87226) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 YDL208W 1 87512 87982 ON 4 NHP2 (87512..87982) Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing YDL207W 1 88248 89864 ON 4 GLE1 (88248..89864) Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export YDL206W 1 90176 92464 ON 4 YDL206W (90176..92464) Putative protein of unknown function; YDL206W is not an essential protein YDL205C 0 92762 93745 ON 4 HEM3 complement(92762..93745) Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme YDL204W 1 94605 95786 ON 4 RTN2 (94605..95786) Reticulon protein that stabilizes membrane curvature; interacts with Rtn1p, Yop1p, and Sey1p to maintain tubular ER morphology; involved in nuclear pore assembly; rtn1 rtn2 yop1 triple mutant shows loss of tubular ER; interacts with exocyst subunit Sec6p, with Yip3p, and with Sbh1p; similar to and much less abundant than Rtn1p; member of the RTNLA (reticulon-like A) subfamily YDL203C 0 96082 97953 ON 4 ACK1 complement(96082..97953) Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria YDL202W 1 98475 99224 ON 4 MRPL11 (98475..99224) Mitochondrial ribosomal protein of the large subunit YDL201W 1 99561 100421 ON 4 TRM8 (99561..100421) Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p YDL200C 0 100501 101067 ON 4 MGT1 complement(100501..101067) DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage YDL199C 0 101290 103353 ON 4 YDL199C complement(101290..103353) Putative transporter, member of the sugar porter family YDL198C 0 103649 104551 ON 4 GGC1 complement(103649..104551) Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family YDL197C 0 104917 106494 ON 4 ASF2 complement(104917..106494) Anti-silencing protein that causes derepression of silent loci when overexpressed YDL196W 1 106741 107070 OFF 4 YDL196W (106741..107070) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 YDL195W 1 107208 111029 ON 4 SEC31 (107208..111029) Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance YDL194W 1 111580 114234 ON 4 SNF3 (111580..114234) Plasma membrane low glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; similar to Rgt2p YDL193W 1 114672 115799 ON 4 NUS1 (114672..115799) Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY YDL192W 1 116321 116866 ON 4 ARF1 (116321..116866) ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p YDL191W 1 117664 118517 ON 4 RPL35A (117664..117666,118158..118517) Protein component of the large (60S) ribosomal subunit, identical to Rpl35Bp and has similarity to rat L35 ribosomal protein YDL190C 0 118707 121592 ON 4 UFD2 complement(118707..121592) Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 YDL189W 1 122216 123589 ON 4 RBS1 (122216..123589) Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain YDL188C 0 123865 124998 ON 4 PPH22 complement(123865..124998) Catalytic subunit of protein phosphatase 2A (PP2A), functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis YDL187C 0 125509 125838 OFF 4 YDL187C complement(125509..125838) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDL186W 1 125616 126449 ON 4 YDL186W (125616..126449) Putative protein of unknown function; YDL186W is not an essential gene YDL185C-A 0 126608 126835 OFF 4 YDL185C-A complement(126608..126835) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDL185W; identified by homology with hemiascomycetous yeast species YDL185W 1 126787 130002 ON 4 VMA1 (126787..130002) Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease YDL184C 0 130407 130484 ON 4 RPL41A complement(130407..130484) Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Bp and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable YDL183C 0 130871 131833 ON 4 YDL183C complement(130871..131833) Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene YDL182W 1 133437 134723 ON 4 LYS20 (133437..134723) Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p YDL181W 1 135179 135436 ON 4 INH1 (135179..135436) Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p; has a calmodulin-binding motif and binds calmodulin in vitro YDL180W 1 135896 137539 ON 4 YDL180W (135896..137539) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YDL179W 1 138291 139205 ON 4 PCL9 (138291..139205) Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p YDL178W 1 139522 141114 ON 4 DLD2 (139522..141114) D-lactate dehydrogenase, located in the mitochondrial matrix YDL177C 0 141209 141721 ON 4 YDL177C complement(141209..141721) Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene YDL176W 1 142097 144223 ON 4 YDL176W (142097..144223) Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene YDL175C 0 144484 145518 ON 4 AIR2 complement(144484..145518) RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; functionally redundant with Air1p; has 5 zinc knuckle motifs YDL174C 0 145826 147589 ON 4 DLD1 complement(145826..147589) D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane YDL173W 1 148191 149078 ON 4 PAR32 (148191..149078) Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene YDL172C 0 148607 149086 OFF 4 YDL172C complement(148607..149086) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL171C 0 149203 155640 ON 4 GLT1 complement(149203..155640) NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source YDL170W 1 156318 157904 ON 4 UGA3 (156318..157904) Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus YDL169C 0 158064 158735 ON 4 UGX2 complement(158064..158735) Protein of unknown function, transcript accumulates in response to any combination of stress conditions YDL168W 1 159604 160764 ON 4 SFA1 (159604..160764) Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p YDL167C 0 160995 163154 ON 4 NRP1 complement(160995..163154) Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis YDL166C 0 163449 164042 ON 4 FAP7 complement(163449..164042) Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D YDL165W 1 164290 164865 ON 4 CDC36 (164290..164865) Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor YDL164C 0 164987 167254 ON 4 CDC9 complement(164987..167254) DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination YDL163W 1 166956 167258 OFF 4 YDL163W (166956..167258) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase YDL162C 0 167359 167715 OFF 4 YDL162C complement(167359..167715) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly YDL161W 1 167714 169078 ON 4 ENT1 (167714..169078) Epsin-like protein involved in endocytosis and actin patch assembly and functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus YDL160C-A 0 169366 169608 ON 4 MHF2 complement(169366..169608) Protein of unknown function; ortholog of human MHF2, and component of the heterotetrameric MHF histone-fold complex that in humans interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive YDL160C 0 170410 171930 ON 4 DHH1 complement(170410..171930) Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay YDL159W-A 1 172182 172313 OFF 4 YDL159W-A (172182..172313) Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species YDL159W 1 172481 174028 ON 4 STE7 (172481..174028) Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway YDL159C-B 0 173056 173232 OFF 4 YDL159C-B complement(173056..173232) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDL158C 0 173866 174174 OFF 4 YDL158C complement(173866..174174) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL157C 0 174232 174588 ON 4 YDL157C complement(174232..174588) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDL156W 1 174918 176486 ON 4 CMR1 (174918..176486) DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the YDL156W promoter region YDL155W 1 176773 178056 ON 4 CLB3 (176773..178056) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation YDL154W 1 178334 181039 ON 4 MSH5 (178334..181039) Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans YDL153C 0 181186 183018 ON 4 SAS10 complement(181186..183018) Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance YDL152W 1 182822 183187 OFF 4 YDL152W (182822..183187) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome YDL151C 0 183318 183899 OFF 4 BUD30 complement(183318..183899) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay YDL150W 1 183343 184611 ON 4 RPC53 (183343..184611) RNA polymerase III subunit C53 YDL149W 1 184925 187918 ON 4 ATG9 (184925..187918) Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS YDL148C 0 188154 190586 ON 4 NOP14 complement(188154..190586) Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA YDL147W 1 190924 192261 ON 4 RPN5 (190924..192261) Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein YDL146W 1 192750 194225 ON 4 LDB17 (192750..194225) Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck YDL145C 0 194571 198176 ON 4 COP1 complement(194571..198176) Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway YDL144C 0 198662 199732 ON 4 YDL144C complement(198662..199732) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. YDL143W 1 199996 201582 ON 4 CCT4 (199996..201582) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YDL142C 0 201719 202570 ON 4 CRD1 complement(201719..202570) Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis YDL141W 1 203039 205111 ON 4 BPL1 (203039..205111) Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation YDL140C 0 205360 210561 ON 4 RPO21 complement(205360..210561) RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime YDL139C 0 211375 212046 ON 4 SCM3 complement(211375..212046) Nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation YDL138W 1 213351 215642 ON 4 RGT2 (213351..215642) Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; highly similar to Snf3p YDL137W 1 216529 217074 ON 4 ARF2 (216529..217074) ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p YDL136W 1 217600 218367 ON 4 RPL35B (217600..217602,218008..218367) Protein component of the large (60S) ribosomal subunit, identical to Rpl35Ap and has similarity to rat L35 ribosomal protein YDL135C 0 218680 219288 ON 4 RDI1 complement(218680..219288) Rho GDP dissociation inhibitor involved in the localization and regulation of Cdc42p and Rho1p YDL134C 0 219662 220771 ON 4 PPH21 complement(219662..220771) Catalytic subunit of protein phosphatase 2A (PP2A), functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis YDL133C-A 0 221724 221801 ON 4 RPL41B complement(221724..221801) Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable YDL133W 1 222427 223740 ON 4 SRF1 (222427..223740) Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients YDL132W 1 224304 226751 ON 4 CDC53 (224304..226751) Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation YDL131W 1 227393 228715 ON 4 LYS21 (227393..228715) Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p YDL130W-A 1 229171 229431 ON 4 STF1 (229171..229431) Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein YDL130W 1 229906 230527 ON 4 RPP1B (229906..230019,230321..230527) Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component YDL129W 1 231024 231899 ON 4 YDL129W (231024..231899) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene YDL128W 1 232652 233887 ON 4 VCX1 (232652..233887) Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter YDL127W 1 234927 235853 ON 4 PCL2 (234927..235853) Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth YDL126C 0 236157 238664 ON 4 CDC48 complement(236157..238664) AAA ATPase involved in multiple processes; subunit of a polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress and mitotic spindle disassembly; subunit of a complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, ribophagy, and homotypic ER membrane fusion; functional ortholog of p97/VCP YDL125C 0 239019 239606 ON 4 HNT1 complement(239019..239398,239510..239606) Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint YDL124W 1 240259 241197 ON 4 YDL124W (240259..241197) NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family YDL123W 1 241418 241840 ON 4 SNA4 (241418..241840) Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated YDL122W 1 242552 244981 ON 4 UBP1 (242552..244981) Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains YDL121C 0 245133 245582 ON 4 YDL121C complement(245133..245582) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein YDL120W 1 245923 246447 ON 4 YFH1 (245923..246447) Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia YDL119C 0 246689 247612 ON 4 YDL119C complement(246689..247612) Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria YDL118W 1 247302 247682 OFF 4 YDL118W (247302..247682) Non-essential protein of unconfirmed function; mutants are defective in telomere maintenance, and are synthetically sick or lethal with alpha-synuclein YDL117W 1 248581 251238 ON 4 CYK3 (248581..251238) SH3-domain protein located in the mother-bud neck and the cytokinetic actin ring; mutant phenotype and genetic interactions suggest a role in cytokinesis YDL116W 1 251566 253746 ON 4 NUP84 (251566..253746) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 YDL115C 0 253995 255126 ON 4 IWR1 complement(253995..254974,255045..255126) RNA polymerase II transport factor, conserved from yeast to humans; involved in both basal and regulated transcription from RNA polymerase II (RNAP II) promoters, but not itself a transcription factor; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin YDL114W-A 1 254935 255048 OFF 4 YDL114W-A (254935..255048) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified based on homology to hemiascomycetous yeasts YDL114W 1 255604 256530 ON 4 YDL114W (255604..256530) Putative protein of unknown function with similarity to acyl-carrier-protein reductases; YDL114W is not an essential gene YDL113C 0 256633 258555 ON 4 ATG20 complement(256633..258555) Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate YDL112W 1 258915 263225 ON 4 TRM3 (258915..263225) 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs YDL111C 0 263313 264110 ON 4 RRP42 complement(263313..264110) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) YDL110C 0 264512 264964 ON 4 TMA17 complement(264512..264964) Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion YDL109C 0 265258 267201 ON 4 YDL109C complement(265258..267201) Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene YDL108W 1 267698 268699 ON 4 KIN28 (267698..267725,267807..268699) Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters YDL107W 1 268921 269976 ON 4 MSS2 (268921..269976) Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p YDL106C 0 270222 271901 ON 4 PHO2 complement(270222..271901) Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p YDL105W 1 272389 273597 ON 4 NSE4 (272389..273597) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair YDL104C 0 273653 274876 ON 4 QRI7 complement(273653..274876) Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification YDL103C 0 275148 276581 ON 4 QRI1 complement(275148..276581) UDP-N-acetylglucosamine pyrophosphorylase, catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis YDL102W 1 276872 280165 ON 4 POL3 (276872..280165) Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) YDL101C 0 280307 281848 ON 4 DUN1 complement(280307..281848) Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair YDL100C 0 282112 283176 ON 4 GET3 complement(282112..283176) Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity YDL099W 1 283419 284444 ON 4 BUG1 (283419..284444) Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes YDL098C 0 284581 285165 ON 4 SNU23 complement(284581..285165) Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome YDL097C 0 285391 286695 ON 4 RPN6 complement(285391..286695) Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion YDL096C 0 286998 287324 OFF 4 OPI6 complement(286998..287324) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential YDL095W 1 287059 289512 ON 4 PMT1 (287059..289512) Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein Ser/Thr residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control YDL094C 0 289572 290081 OFF 4 YDL094C complement(289572..290081) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential YDL093W 1 289909 292140 ON 4 PMT5 (289909..292140) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals YDL092W 1 292781 293221 ON 4 SRP14 (292781..293221) Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 YDL091C 0 293392 294759 ON 4 UBX3 complement(293392..294759) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDL090C 0 295034 296329 ON 4 RAM1 complement(295034..296329) Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit YDL089W 1 296820 298274 ON 4 NUR1 (296820..298274) Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate YDL088C 0 298417 300003 ON 4 ASM4 complement(298417..300003) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport YDL087C 0 300214 300999 ON 4 LUC7 complement(300214..300999) Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA YDL086W 1 301413 302234 ON 4 YDL086W (301413..302234) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene YDL086C-A 0 301655 302089 OFF 4 YDL086C-A complement(301655..302089) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps uncharacterized ORF YDL086W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YDL085C-A 0 302464 302670 ON 4 YDL085C-A complement(302464..302670) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YDL085W 1 303211 304848 ON 4 NDE2 (303211..304848) Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain YDL084W 1 305237 306577 ON 4 SUB2 (305237..306577) Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56 YDL083C 0 306926 307789 ON 4 RPS16B complement(306926..307333,307766..307789) Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins YDL082W 1 308424 309388 ON 4 RPL13A (308424..308427,308793..309388) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential for viability; has similarity to rat L13 ribosomal protein YDL081C 0 309802 310122 ON 4 RPP1A complement(309802..310122) Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component YDL080C 0 310642 312471 ON 4 THI3 complement(310642..312471) Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis YDL079C 0 312951 314748 ON 4 MRK1 complement(312951..314044,314337..314748) Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation YDL078C 0 315357 316388 ON 4 MDH3 complement(315357..316388) Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle YDL077C 0 316971 320120 ON 4 VAM6 complement(316971..320120) Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p YDL076C 0 320668 321552 ON 4 RXT3 complement(320668..321552) Subunit of the RPD3L complex; involved in histone deacetylation YDL075W 1 322226 322988 ON 4 RPL31A (322226..322282,322704..322988) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality Scer_snR63 0 323217 323471 ON 4 SNR63 complement(323217..323471) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A2256 YDL074C 0 324047 326149 ON 4 BRE1 complement(324047..326149) E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control YDL073W 1 326613 329567 ON 4 YDL073W (326613..329567) Putative protein of unknown function; YDL073W is not an essential gene YDL072C 0 329836 330447 ON 4 YET3 complement(329836..330447) Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein YDL071C 0 330666 331040 OFF 4 YDL071C complement(330666..331040) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W YDL070W 1 331024 332940 ON 4 BDF2 (331024..332940) Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p YDL069C 0 333121 333810 ON 4 CBS1 complement(333121..333810) Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader YDL068W 1 333500 333829 OFF 4 YDL068W (333500..333829) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL067C 0 334217 334396 ON 4 COX9 complement(334217..334396) Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain YDL066W 1 334835 336121 ON 4 IDP1 (334835..336121) Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes YDL065C 0 336249 337277 ON 4 PEX19 complement(336249..337277) Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning YDL064W 1 337487 338070 ON 4 UBC9 (337487..337524,337635..338070) SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) YDL063C 0 338272 340134 ON 4 YDL063C complement(338272..340134) Protein required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YDL062W 1 339857 340282 OFF 4 YDL062W (339857..340282) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential YDL061C 0 340628 340798 ON 4 RPS29B complement(340628..340798) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat S29 and E. coli S14 ribosomal proteins YDL060W 1 341619 343985 ON 4 TSR1 (341619..343985) Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p YDL059C 0 344237 344953 ON 4 RAD59 complement(344237..344953) Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p YDL058W 1 345665 351037 ON 4 USO1 (345665..351037) Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex YDL057W 1 351434 352420 ON 4 YDL057W (351434..352420) Putative protein of unknown function; YDL057W is not an essential gene YDL056W 1 352877 355378 ON 4 MBP1 (352877..355378) Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes YDL055C 0 355674 356759 ON 4 PSA1 complement(355674..356759) GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Scer_4.trna2K 1 359577 359672 ON 4 tK-UUU (359577..359613,359637..359672) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YDL054C 0 359825 361285 ON 4 MCH1 complement(359825..361285) Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport YDL053C 0 361699 362256 ON 4 PBP4 complement(361699..362256) Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay YDL052C 0 362672 363583 ON 4 SLC1 complement(362672..363583) 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes YDL051W 1 363952 364779 ON 4 LHP1 (363952..364779) RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen YDL050C 0 364446 364817 OFF 4 YDL050C complement(364446..364817) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL049C 0 365068 365874 ON 4 KNH1 complement(365068..365874) Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance YDL048C 0 366739 368211 ON 4 STP4 complement(366739..368211) Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor YDL047W 1 369771 370706 ON 4 SIT4 (369771..370706) Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization YDL046W 1 371240 371761 ON 4 NPC2 (371240..371761) Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes YDL045W-A 1 372248 372535 ON 4 MRP10 (372248..372535) Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs YDL045C 0 372688 373608 ON 4 FAD1 complement(372688..373608) Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin YDL044C 0 373967 375289 ON 4 MTF2 complement(373967..375289) Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription YDL043C 0 375680 376480 ON 4 PRP11 complement(375680..376480) Subunit of the SF3a splicing factor complex, required for spliceosome assembly YDL042C 0 376757 378445 ON 4 SIR2 complement(376757..378445) Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication YDL041W 1 378102 378455 OFF 4 YDL041W (378102..378455) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C YDL040C 0 378874 381438 ON 4 NAT1 complement(378874..381438) Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing YDL039C 0 381985 384081 OFF 4 PRM7 complement(381985..384081) Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements YDL037C 0 384601 385587 ON 4 BSC1 complement(384601..385587) Protein of unconfirmed function, similar to cell surface flocculin Muc1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression YDL036C 0 387513 388901 ON 4 PUS9 complement(387513..388901) Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence YDL035C 0 389172 392057 ON 4 GPR1 complement(389172..392057) Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis YDL034W 1 391783 392127 OFF 4 YDL034W (391783..392127) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene YDL033C 0 392659 393912 ON 4 SLM3 complement(392659..393912) tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) YDL032W 1 393690 394001 OFF 4 YDL032W (393690..394001) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene YDL031W 1 394217 397204 ON 4 DBP10 (394217..397204) Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis YDL030W 1 397537 399129 ON 4 PRP9 (397537..399129) Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex YDL029W 1 399340 400638 ON 4 ARP2 (399340..399361,399485..400638) Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity YDL028C 0 400997 403291 ON 4 MPS1 complement(400997..403291) Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p YDL027C 0 403695 404957 ON 4 YDL027C complement(403695..404957) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene YDL026W 1 404749 405060 OFF 4 YDL026W (404749..405060) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL025C 0 405344 407206 ON 4 RTK1 complement(405344..407206) Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1 YDL025W-A 1 405410 405514 OFF 4 YDL025W-A (405410..405514) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the ORF YDL025C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YDL024C 0 408451 409857 ON 4 DIA3 complement(408451..409857) Protein of unknown function, involved in invasive and pseudohyphal growth YDL022C-A 0 409808 410056 OFF 4 YDL022C-A complement(409808..410056) Protein of unknown function; partially overlaps the verified gene DIA3; identified by fungal homology and RT-PCR; mRNA identified as translated by ribosome profiling data Scer_4.trna3A 1 410379 410451 ON 4 tA-AGC (410379..410451) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YDL023C 0 411761 412081 OFF 4 YDL023C complement(411761..412081) Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance YDL022W 1 411825 413000 ON 4 GPD1 (411825..413000) NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p YDL021W 1 413953 414888 ON 4 GPM2 (413953..414888) Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event YDL020C 0 415113 416708 ON 4 RPN4 complement(415113..416708) Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses YDL019C 0 417663 421514 ON 4 OSH2 complement(417663..421514) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YDL018C 0 422833 423510 ON 4 ERP3 complement(422833..423510) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport YDL017W 1 424209 425732 ON 4 CDC7 (424209..425732) DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p YDL016C 0 425570 425872 OFF 4 YDL016C complement(425570..425872) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication YDL015C 0 426002 426934 ON 4 TSC13 complement(426002..426934) Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4p YDL014W 1 427364 428347 ON 4 NOP1 (427364..428347) Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin YDL013W 1 429067 430926 ON 4 SLX5 (429067..430926) Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers YDL012C 0 431108 431517 ON 4 YDL012C complement(431108..431386,431473..431517) Tail-anchored plasma membrane protein containing a conserved CYSTM module, possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype YDL011C 0 432308 432631 OFF 4 YDL011C complement(432308..432631) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W YDL010W 1 432330 433025 ON 4 GRX6 (432330..433025) Cis-golgi localized monothiol glutaredoxin that binds an iron-sulfur cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; functional overlap with GRX7 YDL009C 0 432925 433248 OFF 4 YDL009C complement(432925..433248) Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene YDL008W 1 433497 433994 ON 4 APC11 (433497..433994) Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Scer_4.trna4T 0 434264 434336 ON 4 tT-AGU complement(434264..434336) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YDL007C-A 0 436567 436824 ON 4 YDL007C-A complement(436567..436824) Putative protein of unknown function Scer_4.trna5S 1 437772 437853 ON 4 tS-AGA (437772..437853) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YDL007W 1 438047 439360 ON 4 RPT2 (438047..439360) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle YDL006W 1 439909 440754 ON 4 PTC1 (439909..440754) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation YDL005C 0 441014 442309 ON 4 MED2 complement(441014..442309) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YDL004W 1 443029 443511 ON 4 ATP16 (443029..443511) Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated YDL003W 1 444683 446383 ON 4 MCD1 (444683..446383) Essential subunit of the cohesin complex required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase YDL002C 0 446967 447578 ON 4 NHP10 complement(446967..447578) Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair YDL001W 1 447984 449276 ON 4 RMD1 (447984..449276) Cytoplasmic protein required for sporulation Scer_CEN4 1 449711 449821 ON 4 CEN4 (449711..449821) Chromosome IV centromere YDR001C 0 450220 452475 ON 4 NTH1 complement(450220..452475) Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p YDR002W 1 453045 453650 ON 4 YRB1 (453045..453650) Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 YDR003W 1 454122 454754 ON 4 RCR2 (454122..454754) Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; similar to Rcr1p YDR003W-A 1 454782 454904 OFF 4 YDR003W-A (454782..454904) Putative protein of unknown function; identified by expression profiling and mass spectrometry YDR004W 1 455201 456583 ON 4 RAD57 (455201..456583) Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p YDR005C 0 456836 458103 ON 4 MAF1 complement(456836..458017,458098..458103) Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions YDR006C 0 458542 461247 ON 4 SOK1 complement(458542..461247) Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 YDR007W 1 461842 462516 ON 4 TRP1 (461842..462516) Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) YDR008C 0 462252 462602 OFF 4 YDR008C complement(462252..462602) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDR009W 1 463434 464996 ON 4 GAL3 (463434..464996) Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity YDR010C 0 465051 465383 OFF 4 YDR010C complement(465051..465383) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDR011W 1 465919 470424 ON 4 SNQ2 (465919..470424) Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species YDR012W 1 471853 472941 ON 4 RPL4B (471853..472941) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins YDR013W 1 473157 473783 ON 4 PSF1 (473157..473783) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YDR014W 1 474046 475989 ON 4 RAD61 (474046..475989) Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin YDR014W-A 1 477797 478285 ON 4 HED1 (477797..478285) Meiosis-specific protein that down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; early meiotic gene, transcribed specifically during meiotic prophase YDR015C 0 477960 478199 OFF 4 YDR015C complement(477960..478199) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A YDR016C 0 478474 478758 ON 4 DAD1 complement(478474..478758) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YDR017C 0 479115 482267 ON 4 KCS1 complement(479115..482267) Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance YDR018C 0 482670 483860 ON 4 YDR018C complement(482670..483860) Probable membrane protein with three predicted transmembrane domains; homologous to Ybr042cp, similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase YDR019C 0 484163 485365 ON 4 GCV1 complement(484163..485365) T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm YDR020C 0 485746 486444 ON 4 DAS2 complement(485746..486444) Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases YDR021W 1 486804 488003 ON 4 FAL1 (486804..488003) Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases YDR022C 0 488072 488662 ON 4 CIS1 complement(488072..488662) Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Scer_4.trna6V 0 488797 488870 ON 4 tV-UAC complement(488797..488870) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: UAC | Cove Score: 74.06 YDR023W 1 489508 490896 ON 4 SES1 (489508..490896) Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p YDR024W 1 491017 491502 OFF 4 FYV1 (491017..491502) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin YDR025W 1 491515 492324 ON 4 RPS11A (491515..491559,491899..492324) Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins YDR026C 0 492556 494268 ON 4 NSI1 complement(492556..494268) RNA polymerase I termination factor; binds to the rDNA terminator element and is required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities the association of Sir2p with NTS1, and contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein with similarity to Reb1p YDR027C 0 494649 497318 ON 4 VPS54 complement(494649..497318) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p YDR028C 0 497835 500879 ON 4 REG1 complement(497835..500879) Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes YDR029W 1 501100 501414 OFF 4 YDR029W (501100..501414) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDR030C 0 501753 503273 ON 4 RAD28 complement(501753..503273) Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair YDR031W 1 503498 503863 ON 4 MIC14 (503498..503863) Mitochondrial intermembrane space protein, required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs YDR032C 0 504099 504695 ON 4 PST2 complement(504099..504695) Protein with similarity to members of a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR033W 1 508147 509109 ON 4 MRH1 (508147..509109) Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; has similarity to Hsp30p and Yro2p YDR034C 0 509737 512109 ON 4 LYS14 complement(509737..512109) Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Scer_4.trna7L 1 519743 519826 ON 4 tL-UAA (519743..519826) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YDR034C-A 0 520516 520692 OFF 4 YDR034C-A complement(520516..520692) Putative protein of unknown function; contained within the solo Ty1 LTR element YDRWdelta7 Scer_4.trna8Q 1 520972 521043 ON 4 tQ-UUG (520972..521043) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YDR034W-B 1 521314 521469 ON 4 YDR034W-B (521314..521469) Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YDR035W 1 521816 522928 ON 4 ARO3 (521816..522928) 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan YDR036C 0 523211 524713 ON 4 EHD3 complement(523211..524713) 3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis YDR037W 1 525440 527215 ON 4 KRS1 (525440..527215) Lysyl-tRNA synthetase YDR038C 0 527422 530697 ON 4 ENA5 complement(527422..530697) Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family YDR039C 0 531307 534582 ON 4 ENA2 complement(531307..534582) P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux YDR040C 0 535192 538467 ON 4 ENA1 complement(535192..538467) P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance YDR041W 1 539803 540414 ON 4 RSM10 (539803..540414) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins YDR042C 0 540601 541203 ON 4 YDR042C complement(540601..541203) Putative protein of unknown function; expression is increased in ssu72-ts69 mutant Scer_snR47 0 541602 541700 ON 4 SNR47 complement(541602..541700) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A2220 and small subunit (SSU) rRNA at position A619 YDR043C 0 542674 543369 ON 4 NRG1 complement(542674..543369) Transcriptional repressor that recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response YDR044W 1 546642 547628 ON 4 HEM13 (546642..547628) Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) YDR045C 0 547978 548310 ON 4 RPC11 complement(547978..548310) RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS YDR046C 0 548762 550576 ON 4 BAP3 complement(548762..550576) Amino acid permease involved in the uptake of cysteine, leucine, isoleucine and valine YDR047W 1 551860 552948 ON 4 HEM12 (551860..552948) Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda YDR048C 0 553084 553398 OFF 4 YDR048C complement(553084..553398) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR049W 1 553254 555152 ON 4 VMS1 (553254..555152) Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans YDR050C 0 555726 556472 ON 4 TPI1 complement(555726..556472) Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease YDR051C 0 557056 558060 ON 4 DET1 complement(557056..558060) Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel YDR052C 0 558511 560625 ON 4 DBF4 complement(558511..560625) Regulatory subunit of Cdc7p-Dbf4p kinase complex, required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated YDR053W 1 560249 560644 OFF 4 YDR053W (560249..560644) Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex YDR054C 0 561440 562327 ON 4 CDC34 complement(561440..562327) Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation YDR055W 1 563527 564861 ON 4 PST1 (563527..564861) Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 YDR056C 0 565023 565640 ON 4 YDR056C complement(565023..565640) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein YDR057W 1 565927 567555 ON 4 YOS9 (565927..567555) ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family YDR058C 0 567723 568703 ON 4 TGL2 complement(567723..568703) Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Scer_4.trna9R 1 568882 568953 ON 4 tR-UCU (568882..568953) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 Scer_4.trna10D 1 568964 569035 ON 4 tD-GUC (568964..569035) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YDR059C 0 569234 569770 ON 4 UBC5 complement(569234..569633,569724..569770) Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible YDR060W 1 570649 573726 ON 4 MAK21 (570649..573726) Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein YDR061W 1 574164 575783 ON 4 YDR061W (574164..575783) Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance YDR062W 1 576474 578159 ON 4 LCB2 (576474..578159) Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine YDR063W 1 578663 579112 ON 4 AIM7 (578663..579112) Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss YDR064W 1 579458 580452 ON 4 RPS13 (579458..579478,580018..580452) Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins YDR065W 1 580687 581784 ON 4 RRG1 (580687..581784) Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR066C 0 581908 582498 ON 4 RTR2 complement(581908..582498) Protein of unknown function with high similarity to Rtr1p; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene YDR067C 0 582791 583465 ON 4 OCA6 complement(582791..583465) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT YDR068W 1 583713 584645 ON 4 DOS2 (583713..584645) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR069C 0 584939 587719 ON 4 DOA4 complement(584939..587719) Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole YDR070C 0 588098 588379 ON 4 FMP16 complement(588098..588379) Putative protein of unknown function; proposed to be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR071C 0 588827 589402 ON 4 PAA1 complement(588827..589402) Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication YDR072C 0 589761 591344 ON 4 IPT1 complement(589761..591344) Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid;, can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin YDR073W 1 592439 592948 ON 4 SNF11 (592439..592948) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p YDR074W 1 593893 596583 ON 4 TPS2 (593893..596583) Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway YDR075W 1 597156 598082 ON 4 PPH3 (597156..598082) Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes YDR076W 1 598468 599688 ON 4 RAD55 (598468..599688) Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p YDR077W 1 600793 601809 ON 4 SED1 (600793..601809) Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites YDR078C 0 602198 602869 ON 4 SHU2 complement(602198..602869) Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function YDR079W 1 603064 603399 ON 4 PET100 (603064..603399) Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme YDR079C-A 0 603593 603811 ON 4 TFB5 complement(603593..603811) Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair YDR080W 1 604008 606986 ON 4 VPS41 (604008..606986) Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport YDR081C 0 607304 610081 ON 4 PDC2 complement(607304..610081) Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions YDR082W 1 610441 611925 ON 4 STN1 (610441..611925) Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping YDR083W 1 612073 613251 ON 4 RRP8 (612073..613251) Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation YDR084C 0 613405 614004 ON 4 TVP23 complement(613405..614004) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR085C 0 614288 616150 ON 4 AFR1 complement(614288..616150) Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p YDR086C 0 616928 617170 ON 4 SSS1 complement(616928..617170) Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p YDR087C 0 617470 618306 ON 4 RRP1 complement(617470..618306) Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles YDR088C 0 618497 619645 ON 4 SLU7 complement(618497..619645) RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Scer_4.trna11R 0 619969 620041 ON 4 tR-ACG complement(619969..620041) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 71.46 YDR089W 1 622112 624721 ON 4 YDR089W (622112..624721) Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis YDR090C 0 625066 625998 ON 4 YDR090C complement(625066..625998) Putative protein of unknown function YDR091C 0 626708 628534 ON 4 RLI1 complement(626708..628534) Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase YDR092W 1 629876 630605 ON 4 UBC13 (629876..629905,630174..630605) Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus YDR093W 1 631282 636120 ON 4 DNF2 (631282..636120) Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase YDR094W 1 635840 636175 OFF 4 YDR094W (635840..636175) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 YDR095C 0 636117 636527 OFF 4 YDR095C complement(636117..636527) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR096W 1 637139 639823 ON 4 GIS1 (637139..639823) JmjC domain-containing histone demethylase and transcription factor; involved in expression of genes during nutrient limitation; negatively regulates DPP1 and PHR1; activity is modulated by limited proteasome-mediated proteolysis; has a JmjC and a JmjN domain in the N-terminal region that interact, promoting Gis1p stability and proper transcriptional activity; contains transactivating domains TAD1 and TAD2 downstream of the Jmj domains and a C-terminal DNA binding domain YDR097C 0 640109 643837 ON 4 MSH6 complement(640109..643837) Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p YDR098C 0 644178 645035 ON 4 GRX3 complement(644178..645035) Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 Scer_4.trna12Q 0 645153 645224 ON 4 tQ-UUG complement(645153..645224) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YDR099W 1 653607 654428 ON 4 BMH2 (653607..654428) 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling YDR100W 1 655013 655444 ON 4 TVP15 (655013..655444) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p YDR101C 0 655686 657467 ON 4 ARX1 complement(655686..657467) Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex YDR102C 0 657648 657980 OFF 4 YDR102C complement(657648..657980) Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index YDR103W 1 658350 661103 ON 4 STE5 (658350..661103) Pheromone-response scaffold protein that controls the mating decision; binds Ste11p, Ste7p, and Fus3p kinases, forming a MAPK cascade complex that interacts with the plasma membrane and Ste4p-Ste18p; allosteric activator of Fus3p YDR104C 0 661173 664910 ON 4 SPO71 complement(661173..664910) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains YDR105C 0 665349 666770 ON 4 TMS1 complement(665349..666770) Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance YDR106W 1 667004 667858 ON 4 ARP10 (667004..667858) Component of the dynactin complex, localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex Scer_4.trna13I 0 668007 668080 ON 4 tI-AAU complement(668007..668080) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YDR107C 0 669016 671034 ON 4 TMN2 complement(669016..671034) Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments YDR108W 1 671269 673365 ON 4 TRS85 (671269..673365) Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role YDR109C 0 673520 675667 ON 4 YDR109C complement(673520..675667) Putative kinase YDR110W 1 676102 677802 ON 4 FOB1 (676102..677802) Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases YDR111C 0 678241 679764 ON 4 ALT2 complement(678241..679764) Putative alanine transaminase (glutamic pyruvic transaminase) YDR112W 1 679544 679852 OFF 4 IRC2 (679544..679852) Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci YDR113C 0 680496 681617 ON 4 PDS1 complement(680496..681617) Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation YDR114C 0 681924 682226 OFF 4 YDR114C complement(681924..682226) Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium YDR115W 1 682175 682492 ON 4 YDR115W (682175..682492) Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins YDR116C 0 682724 683581 ON 4 MRPL1 complement(682724..683581) Mitochondrial ribosomal protein of the large subunit YDR117C 0 683946 685643 ON 4 TMA64 complement(683946..685643) Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity YDR118W 1 685882 687840 ON 4 APC4 (685882..687840) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis YDR118W-A 1 687768 687884 OFF 4 YDR118W-A (687768..687884) Dubious open reading frame; identified using a combination of expression profiling and mass spectrometry YDR119W 1 688227 690533 ON 4 VBA4 (688227..690533) Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene YDR119W-A 1 691014 691214 ON 4 COX26 (691014..691214) Putative protein of unknown function; copurifies with respiratory chain supercomplexes composed of Complex III (ubiquinol-cytochrome c reductase) and Complex IV (cytochrome c oxidase) YDR120C 0 691549 693261 ON 4 TRM1 complement(691549..693261) tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments YDR121W 1 693585 694175 ON 4 DPB4 (693585..694175) Shared subunit of DNA polymerase (II) epsilon and of ISW2/yCHRAC chromatin accessibility complex; involved in both chromosomal DNA replication and in inheritance of telomeric silencing YDR122W 1 694700 697894 ON 4 KIN1 (694700..697894) Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p YDR123C 0 698554 699468 ON 4 INO2 complement(698554..699468) Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion YDR124W 1 700315 701289 ON 4 YDR124W (700315..701289) Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor YDR125C 0 701397 702758 ON 4 ECM18 complement(701397..702758) Protein of unknown function, similar to Rlp24p YDR126W 1 703234 704244 ON 4 SWF1 (703234..704244) Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion YDR127W 1 704484 709250 ON 4 ARO1 (704484..709250) Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids YDR128W 1 709549 712995 ON 4 MTC5 (709549..712995) Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1 YDR129C 0 713340 715379 ON 4 SAC6 complement(713340..715247,715359..715379) Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton YDR130C 0 715747 716622 ON 4 FIN1 complement(715747..716622) Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation YDR131C 0 716790 718460 ON 4 YDR131C complement(716790..718460) F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex YDR132C 0 718816 720303 ON 4 YDR132C complement(718816..720303) Putative protein of unknown function YDR133C 0 720962 721297 OFF 4 YDR133C complement(720962..721297) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C YDR134C 0 721071 721481 OFF 4 YDR134C complement(721071..721481) Hypothetical protein Scer_YGOB_YDR134C 0 721071 721481 ON 4 YDR134C complement(721071..721280,721284..721481) ANNOTATED BY YGOB - YDR135C 0 723004 727551 ON 4 YCF1 complement(723004..727551) Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR YDR136C 0 727711 728283 OFF 4 VPS61 complement(727711..728283) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect YDR137W 1 728259 730250 ON 4 RGP1 (728259..730250) Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p YDR138W 1 730578 732836 ON 4 HPR1 (730578..732836) Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p YDR139C 0 733618 733924 ON 4 RUB1 complement(733618..733702,733776..733924) Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) YDR140W 1 734138 734803 ON 4 MTQ2 (734138..734803) S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC YDR141C 0 734901 739997 ON 4 DOP1 complement(734901..739997) Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies YDR142C 0 740473 741600 ON 4 PEX7 complement(740473..741600) Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) YDR143C 0 742042 743874 ON 4 SAN1 complement(742042..743874) Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition YDR144C 0 744311 746101 ON 4 MKC7 complement(744311..746101) GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p YDR145W 1 746738 748357 ON 4 TAF12 (746738..748357) Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A YDR146C 0 748613 750742 ON 4 SWI5 complement(748613..750742) Transcription factor that recruits the Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase YDR147W 1 751631 753235 ON 4 EKI1 (751631..753235) Ethanolamine kinase, primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway YDR148C 0 753675 755066 ON 4 KGD2 complement(753675..755066) Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated YDR149C 0 755555 756262 OFF 4 YDR149C complement(755555..756262) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth YDR150W 1 755628 763874 ON 4 NUM1 (755628..763874) Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex YDR151C 0 764178 765155 ON 4 CTH1 complement(764178..765155) Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis YDR152W 1 765706 766503 ON 4 GIR2 (765706..766503) Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein YDR153C 0 766736 767971 ON 4 ENT5 complement(766736..767971) Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin YDR154C 0 768403 768753 OFF 4 YDR154C complement(768403..768753) Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein YDR155C 0 768512 769000 ON 4 CPR1 complement(768512..769000) Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A YDR156W 1 769525 769938 ON 4 RPA14 (769525..769938) RNA polymerase I subunit A14 YDR157W 1 769931 770332 OFF 4 YDR157W (769931..770332) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR158W 1 770357 771454 ON 4 HOM2 (770357..771454) Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis YDR159W 1 771877 775782 ON 4 SAC3 (771877..775782) Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) YDR160W 1 776163 778721 ON 4 SSY1 (776163..778721) Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes YDR161W 1 779043 780206 ON 4 YDR161W (779043..780206) Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) YDR162C 0 780390 781100 ON 4 NBP2 complement(780390..781100) Protein involved in the HOG (high osmolarity glycerol) pathway, negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex, found in the nucleus and cytoplasm, contains an SH3 domain that binds Pbs2p YDR163W 1 781423 781950 ON 4 CWC15 (781423..781950) Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p YDR164C 0 782041 784215 ON 4 SEC1 complement(782041..784215) Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function YDR165W 1 784871 786205 ON 4 TRM82 (784871..786205) Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p YDR166C 0 786306 789221 ON 4 SEC5 complement(786306..789221) Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YDR167W 1 789449 790069 ON 4 TAF10 (789449..790069) Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification YDR168W 1 790328 791848 ON 4 CDC37 (790328..791848) Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding YDR169C 0 792348 793889 ON 4 STB3 complement(792348..793889) Ribosomal RNA processing element (RRPE)-binding protein involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p YDR169C-A 0 794574 794723 OFF 4 YDR169C-A complement(794574..794723) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR170C 0 796193 802222 ON 4 SEC7 complement(796193..802222) Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Scer_4.trna14Q 0 802731 802802 ON 4 tQ-UUG complement(802731..802802) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YDR171W 1 806621 807748 ON 4 HSP42 (806621..807748) Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock YDR172W 1 808324 810381 ON 4 SUP35 (808324..810381) Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype YDR173C 0 810565 811632 ON 4 ARG82 complement(810565..811632) Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes YDR174W 1 812110 812850 ON 4 HMO1 (812110..812850) Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase YDR175C 0 813193 814152 ON 4 RSM24 complement(813193..814152) Mitochondrial ribosomal protein of the small subunit YDR176W 1 814452 816560 ON 4 NGG1 (814452..816560) Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex YDR177W 1 816878 817525 ON 4 UBC1 (816878..817525) Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response YDR178W 1 817950 818495 ON 4 SDH4 (817950..818495) Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain YDR179C 0 818708 819196 ON 4 CSN9 complement(818708..819196) Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling YDR179W-A 1 819433 820824 ON 4 YDR179W-A (819433..820824) Putative protein of unknown function YDR180W 1 821295 825776 ON 4 SCC2 (821295..825776) Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin YDR181C 0 825910 827355 ON 4 SAS4 complement(825910..827355) Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p YDR182W 1 827582 829057 ON 4 CDC1 (827582..829057) Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution YDR182W-A 1 829151 829354 OFF 4 YDR182W-A (829151..829354) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR183W 1 829585 830277 ON 4 PLP1 (829585..830277) Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators YDR183C-A 0 830397 830654 OFF 4 YDR183C-A complement(830397..830654) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YDR184C 0 830629 831513 ON 4 ATC1 complement(830629..831513) Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern YDR185C 0 831934 832473 ON 4 UPS3 complement(831934..832473) Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations YDR186C 0 832859 835492 ON 4 YDR186C complement(832859..835492) Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR187C 0 836228 836746 OFF 4 YDR187C complement(836228..836746) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W YDR188W 1 836421 838061 ON 4 CCT6 (836421..838061) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif YDR189W 1 838392 840392 ON 4 SLY1 (838392..840392) Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex YDR190C 0 840604 841995 ON 4 RVB1 complement(840604..841995) ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly YDR191W 1 842337 843449 ON 4 HST4 (842337..843449) Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism YDR192C 0 843569 844861 ON 4 NUP42 complement(843569..844861) FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p YDR193W 1 844554 844952 OFF 4 YDR193W (844554..844952) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR194C 0 845952 847946 ON 4 MSS116 complement(845952..847946) DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing YDR194W-A 1 848071 848223 OFF 4 YDR194W-A (848071..848223) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR195W 1 848599 850200 ON 4 REF2 (848599..850200) RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes YDR196C 0 850274 850999 ON 4 CAB5 complement(850274..850999) Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies YDR197W 1 851228 852397 ON 4 CBS2 (851228..852397) Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader YDR198C 0 852533 853972 ON 4 RKM2 complement(852533..853972) Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp YDR199W 1 854175 854540 OFF 4 YDR199W (854175..854540) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity YDR200C 0 854279 856093 ON 4 VPS64 complement(854279..856093) Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance YDR201W 1 856317 856814 ON 4 SPC19 (856317..856814) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body YDR202C 0 856898 857953 ON 4 RAV2 complement(856898..857953) Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme YDR203W 1 857682 857999 OFF 4 YDR203W (857682..857999) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR204W 1 858137 859144 ON 4 COQ4 (858137..859144) Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex YDR205W 1 859346 861520 ON 4 MSC2 (859346..861520) Member of the cation diffusion facilitator family, localizes to the endoplasmic reticulum and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids YDR206W 1 862054 864708 ON 4 EBS1 (862054..864708) Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors YDR207C 0 865012 867522 ON 4 UME6 complement(865012..867522) Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p YDR208W 1 868224 870563 ON 4 MSS4 (868224..870563) Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation YDR209C 0 871036 871449 OFF 4 YDR209C complement(871036..871449) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. YDR210W 1 871074 871301 ON 4 YDR210W (871074..871301) Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Scer_4.trna15I 1 884361 884493 ON 4 tI-UAU (884361..884397,884458..884493) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: UAU | Cove Score: 61.82 YDR211W 1 884727 886865 ON 4 GCD6 (884727..886865) Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YDR212W 1 887232 888911 ON 4 TCP1 (887232..888911) Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein YDR213W 1 889751 892492 ON 4 UPC2 (889751..892492) Sterol regulatory element binding protein, induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; Ecm22p homolog; relocates from intracellular membranes to perinuclear foci on sterol depletion YDR214W 1 892875 893927 ON 4 AHA1 (892875..893927) Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock YDR215C 0 894089 894502 OFF 4 YDR215C complement(894089..894502) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein YDR216W 1 895035 899006 ON 4 ADR1 (895035..899006) Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization YDR217C 0 899551 903480 ON 4 RAD9 complement(899551..903480) DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate YDR218C 0 903781 905052 ON 4 SPR28 complement(903781..905052) Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation YDR219C 0 905455 906852 ON 4 MFB1 complement(905455..906852) Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding YDR220C 0 907000 907293 OFF 4 YDR220C complement(907000..907293) Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein YDR221W 1 907330 909438 ON 4 GTB1 (907330..909438) Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p, involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER YDR222W 1 910054 911301 ON 4 YDR222W (910054..911301) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR223W 1 912099 913502 ON 4 CRF1 (912099..913502) Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain YDR224C 0 914317 914712 ON 4 HTB1 complement(914317..914712) Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation YDR225W 1 915530 915928 ON 4 HTA1 (915530..915928) Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p YDR226W 1 916486 917154 ON 4 ADK1 (916486..917154) Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence YDR227W 1 917571 921647 ON 4 SIR4 (917571..921647) Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan YDR228C 0 921926 923806 ON 4 PCF11 complement(921926..923806) mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping YDR229W 1 924785 926146 ON 4 IVY1 (924785..926146) Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase YDR230W 1 926223 926570 OFF 4 YDR230W (926223..926570) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 YDR231C 0 926293 926910 ON 4 COX20 complement(926293..926910) Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase YDR232W 1 927452 929098 ON 4 HEM1 (927452..929098) 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p YDR233C 0 929470 930357 ON 4 RTN1 complement(929470..930357) Reticulon protein that stabilizes membrane curvature; interacts with Rtn2p, Yop1p, and Sey1p to maintain tubular ER morphology; involved in nuclear pore assembly; rtn1 rtn2 yop1 triple mutant shows loss of tubular ER; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, with Yip3p, and with Sbh1p; similar to and more abundant than Rtn2p; member of the RTNLA (reticulon-like A) subfamily YDR234W 1 931129 933210 ON 4 LYS4 (931129..933210) Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway YDR235W 1 933504 935138 ON 4 PRP42 (933504..935138) U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats YDR236C 0 935236 935892 ON 4 FMN1 complement(935236..935892) Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membrane YDR237W 1 936615 937493 ON 4 MRPL7 (936615..937493) Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein YDR238C 0 937895 940816 ON 4 SEC26 complement(937895..940816) Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) YDR239C 0 941057 943420 ON 4 YDR239C complement(941057..943420) Protein of unknown function that may interact with ribosomes, based on co-purification experiments YDR240C 0 943674 945152 ON 4 SNU56 complement(943674..945152) Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex YDR241W 1 945151 945438 OFF 4 BUD26 (945151..945438) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Scer_4.trna16Y 1 946312 946400 ON 4 tY-GUA (946312..946350,946365..946400) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.81 YDR242W 1 946807 948456 ON 4 AMD2 (946807..948456) Putative amidase YDR243C 0 948518 950284 ON 4 PRP28 complement(948518..950284) RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site YDR244W 1 950563 952401 ON 4 PEX5 (950563..952401) Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions YDR245W 1 952800 953981 ON 4 MNN10 (952800..953981) Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family YDR246W 1 954288 954947 ON 4 TRS23 (954288..954947) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 YDR246W-A 1 955133 955333 OFF 4 YDR246W-A (955133..955333) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR247W 1 956013 957398 ON 4 VHS1 (956013..957398) Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p YDR248C 0 957758 958339 ON 4 YDR248C complement(957758..958339) Putative protein of unknown function; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 YDR249C 0 958681 959802 ON 4 YDR249C complement(958681..959802) Putative protein of unknown function YDR250C 0 960086 960361 OFF 4 YDR250C complement(960086..960361) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YDR251W 1 960614 963106 ON 4 PAM1 (960614..963106) Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype YDR252W 1 963412 963861 ON 4 BTT1 (963412..963861) Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 YDR253C 0 963990 964565 ON 4 MET32 complement(963990..964565) Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met31p YDR254W 1 965113 966489 ON 4 CHL4 (965113..966489) Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore subcomplex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p YDR255C 0 966557 967822 ON 4 RMD5 complement(966557..967822) Conserved protein that has an E3-like ubiquitin ligase activity necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; also required for sporulation; has a degenerate RING finger domain YDR256C 0 968133 969680 ON 4 CTA1 complement(968133..969680) Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation YDR257C 0 969990 971474 ON 4 RKM4 complement(969990..971474) Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein YDR258C 0 971808 974243 ON 4 HSP78 complement(971808..974243) Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates YDR259C 0 974631 975782 ON 4 YAP6 complement(974631..975782) Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism YDR260C 0 976717 977229 ON 4 SWM1 complement(976717..977229) Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation YDR261C 0 977521 979209 ON 4 EXG2 complement(977521..979209) Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Scer_4.trna17S 0 980974 981055 ON 4 tS-AGA complement(980974..981055) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 Scer_4.trna18G 1 992832 992902 ON 4 tG-GCC (992832..992902) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YDR262W 1 993134 993952 ON 4 YDR262W (993134..993952) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment YDR263C 0 994242 995534 ON 4 DIN7 complement(994242..995534) Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination YDR264C 0 996029 998323 ON 4 AKR1 complement(996029..998323) Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats YDR265W 1 998864 999877 ON 4 PEX10 (998864..999877) Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders YDR266C 0 1000104 1002023 ON 4 HEL2 complement(1000104..1002023) Protein of unknown function that co-purifies with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; contains a RING finger domain; computational analysis suggests a role as a transcription factor YDR267C 0 1002510 1003502 ON 4 CIA1 complement(1002510..1003502) WD40 repeat protein involved in assembly of cytosolic and nuclear iron-sulfur proteins; similar to the human Ciao1 protein; YDR267C is an essential gene YDR268W 1 1004004 1005143 ON 4 MSW1 (1004004..1005143) Mitochondrial tryptophanyl-tRNA synthetase YDR269C 0 1005665 1005988 OFF 4 YDR269C complement(1005665..1005988) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR270W 1 1005675 1008689 ON 4 CCC2 (1005675..1008689) Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases YDR271C 0 1008395 1008766 OFF 4 YDR271C complement(1008395..1008766) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W YDR272W 1 1009010 1009834 ON 4 GLO2 (1009010..1009834) Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate YDR273W 1 1010176 1011273 ON 4 DON1 (1010176..1011273) Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II YDR274C 0 1011589 1011960 OFF 4 YDR274C complement(1011589..1011960) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR275W 1 1012252 1012959 ON 4 BSC2 (1012252..1012959) Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression YDR276C 0 1013476 1013643 ON 4 PMP3 complement(1013476..1013643) Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential YDR277C 0 1014401 1015702 ON 4 MTH1 complement(1014401..1015702) Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation YDR278C 0 1017001 1017318 OFF 4 YDR278C complement(1017001..1017318) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_4.trna19E 0 1017207 1017278 ON 4 tE-CUC complement(1017207..1017278) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; tRNA Type: Glu (E) | Anti-Codon: CUC | Cove Score: 59.79 YDR279W 1 1019368 1020420 ON 4 RNH202 (1019368..1020420) Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome YDR280W 1 1020747 1021664 ON 4 RRP45 (1020747..1021664) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9) YDR281C 0 1022007 1022321 ON 4 PHM6 complement(1022007..1022321) Protein of unknown function, expression is regulated by phosphate levels YDR282C 0 1023511 1024755 ON 4 YDR282C complement(1023511..1024755) Putative protein of unknown function YDR283C 0 1025070 1030049 ON 4 GCN2 complement(1025070..1030049) Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control YDR284C 0 1030550 1031419 ON 4 DPP1 complement(1030550..1031419) Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism YDR285W 1 1032436 1035063 ON 4 ZIP1 (1032436..1035063) Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate YDR286C 0 1035231 1035575 ON 4 YDR286C complement(1035231..1035575) Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site YDR287W 1 1035995 1036873 ON 4 INM2 (1035995..1036873) Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy YDR288W 1 1037195 1038106 ON 4 NSE3 (1037195..1038106) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair YDR289C 0 1038280 1039509 ON 4 RTT103 complement(1038280..1039509) Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition YDR290W 1 1039370 1039699 OFF 4 YDR290W (1039370..1039699) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 YDR291W 1 1039728 1042961 ON 4 HRQ1 (1039728..1042961) Putative RecQ helicase; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) YDR292C 0 1043146 1045011 ON 4 SRP101 complement(1043146..1045011) Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p YDR293C 0 1045640 1049392 ON 4 SSD1 complement(1045640..1049392) Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function YDR294C 0 1050459 1052228 ON 4 DPL1 complement(1050459..1052228) Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate YDR295C 0 1052623 1054647 ON 4 HDA2 complement(1052623..1054647) Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance YDR296W 1 1055212 1055892 ON 4 MHR1 (1055212..1055892) Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress YDR297W 1 1056551 1057600 ON 4 SUR2 (1056551..1057600) Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis YDR298C 0 1058176 1058814 ON 4 ATP5 complement(1058176..1058814) Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated YDR299W 1 1059627 1061231 ON 4 BFR2 (1059627..1061231) Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock YDR300C 0 1061505 1062791 ON 4 PRO1 complement(1061505..1062791) Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis YDR301W 1 1063352 1067425 ON 4 CFT1 (1063352..1067425) RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF YDR302W 1 1067731 1068390 ON 4 GPI11 (1067731..1068390) ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog YDR303C 0 1068729 1071386 ON 4 RSC3 complement(1068729..1071386) Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p YDR304C 0 1071880 1072557 ON 4 CPR5 complement(1071880..1072557) Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER YDR305C 0 1072746 1073488 ON 4 HNT2 complement(1072746..1073312,1073402..1073488) Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins YDR306C 0 1073735 1075171 ON 4 YDR306C complement(1073735..1075171) F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Scer_4.trna20V 0 1075472 1075544 ON 4 tV-CAC complement(1075472..1075544) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: CAC | Cove Score: 75.97 YDR307W 1 1075865 1077853 ON 4 PMT7 (1075865..1077853) Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation YDR308C 0 1078027 1078449 ON 4 SRB7 complement(1078027..1078449) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p YDR309C 0 1079048 1080199 ON 4 GIC2 complement(1079048..1080199) Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region YDR310C 0 1081128 1084316 ON 4 SUM1 complement(1081128..1084316) Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint YDR311W 1 1085065 1086993 ON 4 TFB1 (1085065..1086993) Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators YDR312W 1 1087581 1088942 ON 4 SSF2 (1087581..1088942) Protein required for ribosomal large subunit maturation, functionally redundant with Ssf1p; member of the Brix family YDR313C 0 1089219 1090079 ON 4 PIB1 complement(1089219..1090079) RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain YDR314C 0 1090433 1092511 ON 4 RAD34 complement(1090433..1092511) Protein involved in nucleotide excision repair (NER); homologous to RAD4 YDR315C 0 1092741 1093586 ON 4 IPK1 complement(1092741..1093586) Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable YDR316W 1 1093763 1095178 ON 4 OMS1 (1093763..1095178) Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Scer_4.trna21F 0 1095370 1095461 ON 4 tF-GAA complement(1095370..1095405,1095425..1095461) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 71.83 YDR317W 1 1102184 1103428 ON 4 HIM1 (1102184..1103428) Protein of unknown function involved in DNA repair YDR318W 1 1103758 1104947 ON 4 MCM21 (1103758..1103809,1103893..1104947) Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules YDR319C 0 1105003 1105827 ON 4 YDR319C complement(1105003..1105827) Putative protein of unknown function, identified as an ortholog of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens YDR320C 0 1106095 1108101 ON 4 SWA2 complement(1106095..1108101) Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles YDR320C-A 0 1108280 1108498 ON 4 DAD4 complement(1108280..1108498) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YDR320W-B 1 1108484 1108621 OFF 4 YDR320W-B (1108484..1108621) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DAD4/YDR320C-A YDR321W 1 1108702 1109847 ON 4 ASP1 (1108702..1109847) Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively YDR322W 1 1110589 1111692 ON 4 MRPL35 (1110589..1111692) Mitochondrial ribosomal protein of the large subunit YDR322C-A 0 1112003 1112293 ON 4 TIM11 complement(1112003..1112293) Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase YDR323C 0 1112480 1114027 ON 4 PEP7 complement(1112480..1114027) Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance YDR324C 0 1114433 1116763 ON 4 UTP4 complement(1114433..1116763) Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript YDR325W 1 1117126 1120233 ON 4 YCG1 (1117126..1120233) Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus YDR326C 0 1120609 1124925 ON 4 YSP2 complement(1120609..1124925) Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification YDR327W 1 1125302 1125628 OFF 4 YDR327W (1125302..1125628) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 YDR328C 0 1125434 1126018 ON 4 SKP1 complement(1125434..1126018) Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase YDR329C 0 1126270 1127595 ON 4 PEX3 complement(1126270..1127595) Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p YDR330W 1 1127872 1129374 ON 4 UBX5 (1127872..1129374) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p YDR331W 1 1129588 1130823 ON 4 GPI8 (1129588..1130823) ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog YDR332W 1 1131001 1133070 ON 4 IRC3 (1131001..1133070) Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YDR333C 0 1133260 1135431 ON 4 YDR333C complement(1133260..1135431) Putative protein of unknown function YDR334W 1 1135932 1140476 ON 4 SWR1 (1135932..1140476) Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YDR335W 1 1141168 1144842 ON 4 MSN5 (1141168..1144842) Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm YDR336W 1 1145092 1146036 ON 4 YDR336W (1145092..1146036) Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene YDR337W 1 1146319 1147179 ON 4 MRPS28 (1146319..1147179) Mitochondrial ribosomal protein of the small subunit YDR338C 0 1147379 1149466 ON 4 YDR338C complement(1147379..1149466) Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily YDR339C 0 1149951 1150520 ON 4 FCF1 complement(1149951..1150520) Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Scer_4.trna22X 0 1150842 1150941 ON 4 tX-??? complement(1150842..1150941) tRNA of undetermined specificity, predicted by tRNAscan-SE analysis; very similar to serine tRNAs; tRNA Type: Undet (X) | Anti-Codon: ??? | Cove Score: 57.54 YDR340W 1 1150881 1151183 OFF 4 YDR340W (1150881..1151183) Putative protein of unknown function YDR341C 0 1151803 1153626 ON 4 YDR341C complement(1151803..1153626) Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR342C 0 1154216 1155928 ON 4 HXT7 complement(1154216..1155928) High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels YDR343C 0 1159608 1161320 ON 4 HXT6 complement(1159608..1161320) High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3 YDR344C 0 1162006 1162449 OFF 4 YDR344C complement(1162006..1162449) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR345C 0 1162957 1164660 ON 4 HXT3 complement(1162957..1164660) Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions YDR346C 0 1167214 1168659 ON 4 SVF1 complement(1167214..1168659) Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance YDR347W 1 1169178 1170143 ON 4 MRP1 (1169178..1170143) Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein YDR348C 0 1170326 1171825 ON 4 PAL1 complement(1170326..1171825) Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein YDR349C 0 1172386 1174176 ON 4 YPS7 complement(1172386..1174176) Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Scer_4.trna23M 1 1175829 1175901 ON 4 tM-CAU (1175829..1175901) Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 70.03 YDR350C 0 1176120 1178174 ON 4 ATP22 complement(1176120..1178174) Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane YDR351W 1 1178666 1181260 ON 4 SBE2 (1178666..1181260) Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth YDR352W 1 1181801 1182754 ON 4 YDR352W (1181801..1182754) Putative protein of unknown function YDR353W 1 1183299 1184258 ON 4 TRR1 (1183299..1184258) Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress YDR354W 1 1184747 1185889 ON 4 TRP4 (1184747..1185889) Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis YDR354C-A 0 1185139 1185282 OFF 4 YDR354C-A complement(1185139..1185282) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; completely overlaps TRP4; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR355C 0 1186067 1186369 OFF 4 YDR355C complement(1186067..1186369) Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W YDR356W 1 1186107 1188941 ON 4 SPC110 (1186107..1188941) Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner YDR357C 0 1189199 1189567 ON 4 CNL1 complement(1189199..1189567) Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR358W 1 1190059 1191732 ON 4 GGA1 (1190059..1191732) Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi YDR359C 0 1191936 1194884 ON 4 EAF1 complement(1191936..1194884) Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 YDR360W 1 1194598 1195032 OFF 4 OPI7 (1194598..1195032) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. YDR361C 0 1195412 1196263 ON 4 BCP1 complement(1195412..1196263) Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport YDR362C 0 1196679 1198697 ON 4 TFC6 complement(1196679..1198697) One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 YDR363W 1 1199183 1200553 ON 4 ESC2 (1199183..1200553) Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Scer_4.trna24K 1 1201750 1201822 ON 4 tK-CUU (1201750..1201822) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YDR363W-A 1 1202128 1202397 ON 4 SEM1 (1202128..1202397) Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1 YDR364C 0 1202843 1204210 ON 4 CDC40 complement(1202843..1204210) Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats YDR365C 0 1204497 1206383 ON 4 ESF1 complement(1204497..1206383) Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels YDR366C 0 1212438 1212836 OFF 4 YDR366C complement(1212438..1212836) Putative protein of unknown function YDR367W 1 1212848 1213614 ON 4 KEI1 (1212848..1212877,1212979..1213614) Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene YDR368W 1 1213904 1214842 ON 4 YPR1 (1213904..1214842) NADPH-dependent aldo-keto reductase, reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases YDR369C 0 1215016 1217580 ON 4 XRS2 complement(1215016..1217580) Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling YDR370C 0 1217782 1219110 ON 4 YDR370C complement(1217782..1219110) Putative protein of unknown function YDR371W 1 1219413 1220948 ON 4 CTS2 (1219413..1220948) Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect YDR371C-A 0 1219509 1219613 OFF 4 YDR371C-A complement(1219509..1219613) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completeley overlaps the uncharacterized gene CTS2/YDR371W; identified by expression profiling and mass spectrometry YDR372C 0 1221112 1222149 ON 4 VPS74 complement(1221112..1222149) Protein required for Golgi localization of glycosyltransferases; binds the cytosolic domains of Golgi glycosyltransferases; binding to PtdIns4P required for Golgi targeting and function; tetramer formation required for function YDR373W 1 1222759 1223331 ON 4 FRQ1 (1222759..1223331) N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily YDR374C 0 1223474 1224394 ON 4 YDR374C complement(1223474..1224394) Putative protein of unknown function YDR374W-A 1 1224757 1225026 ON 4 WIP1 (1224757..1225026) Putative protein of unknown function YDR375C 0 1225166 1226536 ON 4 BCS1 complement(1225166..1226536) Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases YDR376W 1 1226822 1228303 ON 4 ARH1 (1226822..1228303) Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability YDR377W 1 1228611 1228916 ON 4 ATP17 (1228611..1228916) Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YDR378C 0 1229349 1229609 ON 4 LSM6 complement(1229349..1229609) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YDR379W 1 1230167 1233196 ON 4 RGA2 (1230167..1233196) GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p YDR379C-A 0 1233278 1233517 ON 4 YDR379C-A complement(1233278..1233517) Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy YDR380W 1 1234218 1236125 ON 4 ARO10 (1234218..1236125) Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway YDR381W 1 1236558 1238004 ON 4 YRA1 (1236558..1236842,1237609..1238004) RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member YDR381C-A 0 1238312 1238850 ON 4 YDR381C-A complement(1238312..1238630,1238825..1238850) Protein of unknown function, localized to the mitochondrial outer membrane YDR382W 1 1239492 1239824 ON 4 RPP2B (1239492..1239824) Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm YDR383C 0 1239960 1240676 ON 4 NKP1 complement(1239960..1240676) Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) YDR384C 0 1241204 1242031 ON 4 ATO3 complement(1241204..1242031) Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters YDR385W 1 1243230 1245758 ON 4 EFT2 (1243230..1245758) Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin YDR386W 1 1246084 1247982 ON 4 MUS81 (1246084..1247982) Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p YDR387C 0 1248154 1249821 ON 4 YDR387C complement(1248154..1249821) Putative transporter, member of the sugar porter family; YDR387C is not an essential gene YDR388W 1 1250186 1251634 ON 4 RVS167 (1250186..1251634) Actin-associated protein with roles in endocytosis and exocytosis; interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin YDR389W 1 1252537 1254501 ON 4 SAC7 (1252537..1254501) GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate YDR390C 0 1254937 1256847 ON 4 UBA2 complement(1254937..1256847) Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Scer_4.trna25G 0 1257008 1257079 ON 4 tG-CCC complement(1257008..1257079) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: CCC | Cove Score: 75.60 YDR391C 0 1257358 1258056 ON 4 YDR391C complement(1257358..1258056) Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YDR392W 1 1258696 1259709 ON 4 SPT3 (1258696..1259709) Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters YDR393W 1 1259901 1261271 ON 4 SHE9 (1259901..1261271) Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex YDR394W 1 1261681 1262967 ON 4 RPT3 (1261681..1262967) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B YDR395W 1 1263324 1266158 ON 4 SXM1 (1263324..1266158) Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 YDR396W 1 1266295 1266795 OFF 4 YDR396W (1266295..1266795) Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex YDR397C 0 1266366 1266898 ON 4 NCB2 complement(1266366..1266769,1266862..1266898) Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta YDR398W 1 1267471 1269402 ON 4 UTP5 (1267471..1269402) Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit YDR399W 1 1270068 1270733 ON 4 HPT1 (1270068..1270733) Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome YDR400W 1 1271063 1272085 ON 4 URH1 (1271063..1272085) Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways YDR401W 1 1272223 1272786 OFF 4 YDR401W (1272223..1272786) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR402C 0 1272234 1273703 ON 4 DIT2 complement(1272234..1273703) N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s YDR403W 1 1274602 1276212 ON 4 DIT1 (1274602..1276212) Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure YDR404C 0 1276654 1277169 ON 4 RPB7 complement(1276654..1277169) RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation YDR405W 1 1277646 1278437 ON 4 MRP20 (1277646..1278437) Mitochondrial ribosomal protein of the large subunit YDR406W 1 1279210 1283799 ON 4 PDR15 (1279210..1283799) Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element YDR406W-A 1 1283799 1284044 OFF 4 YDR406W-A (1283799..1284044) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YDR407C 0 1284069 1287938 ON 4 TRS120 complement(1284069..1287938) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic YDR408C 0 1288215 1288859 ON 4 ADE8 complement(1288215..1288859) Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway YDR409W 1 1289406 1292120 ON 4 SIZ1 (1289406..1292120) SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring YDR410C 0 1292372 1293091 ON 4 STE14 complement(1292372..1293091) Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane YDR411C 0 1293369 1294394 ON 4 DFM1 complement(1293369..1294394) Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p YDR412W 1 1294693 1295400 ON 4 RRP17 (1294693..1295400) Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA YDR413C 0 1294836 1295411 OFF 4 YDR413C complement(1294836..1295411) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing YDR414C 0 1295598 1296686 ON 4 ERD1 complement(1295598..1296686) Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) YDR415C 0 1297037 1298161 ON 4 YDR415C complement(1297037..1298161) Putative protein of unknown function YDR416W 1 1298432 1301011 ON 4 SYF1 (1298432..1301011) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans YDR417C 0 1301555 1301926 OFF 4 YDR417C complement(1301555..1301926) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W YDR418W 1 1301616 1302113 ON 4 RPL12B (1301616..1302113) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins YDR419W 1 1303174 1305072 ON 4 RAD30 (1303174..1305072) DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; mutations in human pol eta are responsible for XPV Scer_4.trna26S 1 1305630 1305712 ON 4 tS-AGA (1305630..1305712) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 84.84 YDR420W 1 1306267 1311675 ON 4 HKR1 (1306267..1311675) Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection YDR421W 1 1312040 1314892 ON 4 ARO80 (1312040..1314892) Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids YDR422C 0 1315326 1317773 ON 4 SIP1 complement(1315326..1317773) Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions YDR423C 0 1318046 1319275 ON 4 CAD1 complement(1318046..1319275) AP-1-like basic leucine zipper (bZIP) transcriptional activator involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA YDR424C 0 1319387 1319841 ON 4 DYN2 complement(1319387..1319617,1319698..1319720,1319817..1319841) Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex YDR425W 1 1320064 1321941 ON 4 SNX41 (1320064..1321941) Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p YDR426C 0 1321629 1322006 OFF 4 YDR426C complement(1321629..1322006) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 YDR427W 1 1322205 1323386 ON 4 RPN9 (1322205..1323386) Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects YDR428C 0 1323454 1324239 ON 4 BNA7 complement(1323454..1324239) Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine YDR429C 0 1324477 1325301 ON 4 TIF35 complement(1324477..1325301) eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation YDR430C 0 1325501 1328470 ON 4 CYM1 complement(1325501..1328470) Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology YDR431W 1 1328390 1328701 OFF 4 YDR431W (1328390..1328701) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR432W 1 1328783 1330027 ON 4 NPL3 (1328783..1330027) RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm YDR433W 1 1329600 1330040 OFF 4 YDR433W (1329600..1330040) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR434W 1 1331237 1332841 ON 4 GPI17 (1331237..1332841) Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog YDR435C 0 1332983 1333969 ON 4 PPM1 complement(1332983..1333969) Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits YDR436W 1 1334821 1336953 ON 4 PPZ2 (1334821..1336953) Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance YDR437W 1 1337352 1337774 ON 4 GPI19 (1337352..1337774) Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P YDR438W 1 1338274 1339386 ON 4 THI74 (1338274..1339386) Mitochondrial transporter repressible by thiamine YDR439W 1 1339676 1340719 ON 4 LRS4 (1339676..1340719) Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation YDR440W 1 1342493 1344241 ON 4 DOT1 (1342493..1344241) Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response YDR441C 0 1344517 1345062 ON 4 APT2 complement(1344517..1345062) Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity YDR442W 1 1345647 1346039 OFF 4 YDR442W (1345647..1346039) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR443C 0 1345676 1349938 ON 4 SSN2 complement(1345676..1349938) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation YDR444W 1 1350290 1352353 ON 4 YDR444W (1350290..1352353) Putative protein of unknown function YDR445C 0 1352182 1352589 OFF 4 YDR445C complement(1352182..1352589) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_4.trna27K 0 1352466 1352538 ON 4 tK-CUU complement(1352466..1352538) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YDR446W 1 1353725 1354633 ON 4 ECM11 (1353725..1354633) Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure YDR447C 0 1354829 1355553 ON 4 RPS17B complement(1354829..1355236,1355551..1355553) Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similarity to rat S17 ribosomal protein YDR448W 1 1356065 1357369 ON 4 ADA2 (1356065..1357369) Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes YDR449C 0 1357580 1358902 ON 4 UTP6 complement(1357580..1358902) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YDR450W 1 1359923 1360798 ON 4 RPS18A (1359923..1359969,1360405..1360798) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins YDR451C 0 1361120 1362181 ON 4 YHP1 complement(1361120..1362181) One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval YDR452W 1 1362878 1364902 ON 4 PPN1 (1362878..1364902) Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer YDR453C 0 1365072 1365662 ON 4 TSA2 complement(1365072..1365662) Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants YDR454C 0 1366264 1366827 ON 4 GUK1 complement(1366264..1366827) Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins YDR455C 0 1367373 1367681 OFF 4 YDR455C complement(1367373..1367681) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W YDR456W 1 1367485 1369386 ON 4 NHX1 (1367485..1369386) Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion YDR457W 1 1369790 1379596 ON 4 TOM1 (1369790..1379596) E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones YDR458C 0 1380055 1382046 ON 4 HEH2 complement(1380055..1382046) Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle YDR459C 0 1382319 1383443 ON 4 PFA5 complement(1382319..1383443) Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain YDR460W 1 1383811 1384776 ON 4 TFB3 (1383811..1384776) Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit YDR461W 1 1385176 1385286 ON 4 MFA1 (1385176..1385286) Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 YDR461C-A 0 1385524 1385766 ON 4 YDR461C-A complement(1385524..1385766) Putative protein of unknown function YDR462W 1 1386073 1386516 ON 4 MRPL28 (1386073..1386516) Mitochondrial ribosomal protein of the large subunit YDR463W 1 1386816 1388375 ON 4 STP1 (1386816..1388375) Transcription factor, undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing YDR464W 1 1388872 1393179 ON 4 SPP41 (1388872..1393179) Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 YDR464C-A 0 1392492 1392680 OFF 4 YDR464C-A complement(1392492..1392680) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR465C 0 1393336 1394574 ON 4 RMT2 complement(1393336..1394574) Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67 YDR466W 1 1395121 1397817 ON 4 PKH3 (1395121..1397817) Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant YDR467C 0 1397583 1397909 OFF 4 YDR467C complement(1397583..1397909) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR468C 0 1398026 1398700 ON 4 TLG1 complement(1398026..1398700) Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p YDR469W 1 1399015 1399542 ON 4 SDC1 (1399015..1399542) Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 YDR470C 0 1399706 1401214 ON 4 UGO1 complement(1399706..1401214) Outer membrane component of the mitochondrial fusion machinery; binds directly to Fzo1p and Mgm1p and thus links these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but is not likely to function as a transmembrane transporter YDR471W 1 1401770 1402564 ON 4 RPL27B (1401770..1401800,1402185..1402564) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Ap and has similarity to rat L27 ribosomal protein Scer_snR13 1 1402919 1403042 ON 4 SNR13 (1402919..1403042) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at positions A2280 and A2281 YDR472W 1 1403322 1404173 ON 4 TRS31 (1403322..1404173) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic YDR473C 0 1404445 1405854 ON 4 PRP3 complement(1404445..1405854) Splicing factor, component of the U4/U6-U5 snRNP complex YDR475C 0 1407464 1410094 ON 4 JIP4 complement(1407464..1410094) Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence YDR476C 0 1410453 1411127 ON 4 YDR476C complement(1410453..1411127) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene YDR477W 1 1412373 1414274 ON 4 SNF1 (1412373..1414274) AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis YDR478W 1 1414575 1415171 ON 4 SNM1 (1414575..1415171) Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP YDR479C 0 1415210 1416874 ON 4 PEX29 complement(1415210..1416874) Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p YDR480W 1 1417399 1418370 ON 4 DIG2 (1417399..1418370) MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p YDR481C 0 1418550 1420250 ON 4 PHO8 complement(1418550..1420250) Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN YDR482C 0 1420431 1420838 ON 4 CWC21 complement(1420431..1420838) Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p YDR483W 1 1421157 1422485 ON 4 KRE2 (1421157..1422485) Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation YDR484W 1 1422763 1424688 ON 4 VPS52 (1422763..1424688) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin YDR485C 0 1424820 1427207 ON 4 VPS72 complement(1424820..1427207) Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting YDR486C 0 1427431 1428120 ON 4 VPS60 complement(1427431..1428120) Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p YDR487C 0 1428354 1428980 ON 4 RIB3 complement(1428354..1428980) 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration YDR488C 0 1429188 1430789 ON 4 PAC11 complement(1429188..1430789) Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 YDR489W 1 1431012 1431896 ON 4 SLD5 (1431012..1431896) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YDR490C 0 1431968 1434268 ON 4 PKH1 complement(1431968..1434268) Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p YDR491C 0 1434500 1434991 OFF 4 YDR491C complement(1434500..1434991) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR492W 1 1434924 1435874 ON 4 IZH1 (1434924..1435874) Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc YDR493W 1 1436217 1436588 ON 4 MZM1 (1436217..1436588) Protein required for assembly of the cytochrome bc(1) complex; facilitates insertion of Rip1p into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; conserved in metazoans YDR494W 1 1436930 1438015 ON 4 RSM28 (1436930..1438015) Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation YDR495C 0 1438115 1441150 ON 4 VPS3 complement(1438115..1441150) Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase YDR496C 0 1441433 1443403 ON 4 PUF6 complement(1441433..1443403) Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis YDR497C 0 1443713 1445467 ON 4 ITR1 complement(1443713..1445467) Myo-inositol transporter with strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p YDR498C 0 1445843 1446994 ON 4 SEC20 complement(1445843..1446994) Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p YDR499W 1 1447830 1450073 ON 4 LCD1 (1447830..1450073) Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP YDR500C 0 1450198 1450853 ON 4 RPL37B complement(1450198..1450457,1450847..1450853) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Ap and to rat L37 ribosomal protein YDR501W 1 1451353 1452918 ON 4 PLM2 (1451353..1452918) Forkhead Associated domain containing protein and putative transcription factor found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; similar to TOS4 YDR502C 0 1453310 1454464 ON 4 SAM2 complement(1453310..1454464) S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) YDR503C 0 1455042 1455866 ON 4 LPP1 complement(1455042..1455866) Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA YDR504C 0 1456311 1456694 OFF 4 SPG3 complement(1456311..1456694) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources YDR505C 0 1456695 1459220 ON 4 PSP1 complement(1456695..1459220) Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition YDR506C 0 1459728 1461554 ON 4 GMC1 complement(1459728..1461554) Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein Scer_4.trna28L 0 1461715 1461829 ON 4 tL-CAA complement(1461715..1461758,1461792..1461829) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.09 YDR507C 0 1462358 1465786 ON 4 GIN4 complement(1462358..1465786) Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p YDR508C 0 1466453 1468444 ON 4 GNP1 complement(1466453..1468444) High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids YDR509W 1 1468226 1468573 OFF 4 YDR509W (1468226..1468573) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR510W 1 1469400 1469705 ON 4 SMT3 (1469400..1469705) Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 YDR510C-A 0 1469695 1469811 OFF 4 YDR510C-A complement(1469695..1469811) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR511W 1 1470017 1470418 ON 4 ACN9 (1470017..1470418) Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes YDR512C 0 1470500 1471063 ON 4 EMI1 complement(1470500..1471063) Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs YDR513W 1 1471017 1471448 ON 4 GRX2 (1471017..1471448) Cytoplasmic glutaredoxin, thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress YDR514C 0 1471548 1472999 ON 4 YDR514C complement(1471548..1472999) Protein of unknown function that localizes to mitochondria; overexpression of YDR514C affects endocytic protein trafficking YDR515W 1 1473429 1474772 ON 4 SLF1 (1473429..1474772) RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts YDR516C 0 1474974 1476476 ON 4 EMI2 complement(1474974..1476476) Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression is regulated by glucose-repression transcription factors Mig1/2p YDR517W 1 1477239 1478357 ON 4 GRH1 (1477239..1478357) Acetylated, cis-golgi localized protein involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes YDR518W 1 1478608 1480161 ON 4 EUG1 (1478608..1480161) Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER YDR519W 1 1480425 1480832 ON 4 FPR2 (1480425..1480832) Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking YDR520C 0 1481085 1483403 ON 4 URC2 complement(1481085..1483403) Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism YDR521W 1 1483141 1483476 OFF 4 YDR521W (1483141..1483476) Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol YDR522C 0 1483795 1485303 ON 4 SPS2 complement(1483795..1485303) Protein expressed during sporulation, redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component YDR523C 0 1485566 1487038 ON 4 SPS1 complement(1485566..1487038) Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis YDR524C 0 1487542 1488990 ON 4 AGE1 complement(1487542..1488990) ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif YDR524W-C 1 1489403 1489492 OFF 4 YDR524W-C (1489403..1489492) Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin YDR524C-B 0 1489598 1489798 ON 4 YDR524C-B complement(1489598..1489798) Putative protein of unknown function YDR524C-A 0 1489871 1489990 OFF 4 YDR524C-A complement(1489871..1489990) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR525W 1 1489905 1490234 OFF 4 API2 (1489905..1490234) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology YDR525W-A 1 1490596 1490835 ON 4 SNA2 (1490596..1490835) Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR526C 0 1491075 1491545 OFF 4 YDR526C complement(1491075..1491545) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR527W 1 1491094 1492413 ON 4 RBA50 (1491094..1492413) Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Scer_snR84 0 1492477 1493026 ON 4 SNR84 complement(1492477..1493026) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2266 YDR528W 1 1494586 1495857 ON 4 HLR1 (1494586..1495857) Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A YDR529C 0 1496165 1496548 ON 4 QCR7 complement(1496165..1496548) Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly YDR530C 0 1496791 1497768 ON 4 APA2 complement(1496791..1497768) Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p YDR531W 1 1498232 1499335 ON 4 CAB1 (1498232..1499335) Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element YDR532C 0 1499396 1500553 ON 4 KRE28 complement(1499396..1500553) Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components YDR533C 0 1501447 1502160 ON 4 HSP31 complement(1501447..1502160) Possible chaperone and cysteine protease with similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site YDR534C 0 1503314 1504900 ON 4 FIT1 complement(1503314..1504900) Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall YDR535C 0 1506606 1507362 OFF 4 YDR535C complement(1506606..1507058,1507315..1507362) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. YDR536W 1 1508005 1509714 ON 4 STL1 (1508005..1509714) Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock YDR537C 0 1510856 1511461 OFF 4 YDR537C complement(1510856..1511461) Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W YDR538W 1 1510902 1511630 ON 4 PAD1 (1510902..1511630) Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX YDR539W 1 1512094 1513605 ON 4 FDC1 (1512094..1513605) Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm YDR540C 0 1517136 1517675 ON 4 IRC4 complement(1517136..1517675) Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YDR541C 0 1519664 1520698 ON 4 YDR541C complement(1519664..1520698) Putative dihydrokaempferol 4-reductase YDR542W 1 1523249 1523611 ON 4 PAU10 (1523249..1523611) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YDR543C 0 1524634 1524933 OFF 4 YDR543C complement(1524634..1524933) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR544C 0 1525095 1525523 OFF 4 YDR544C complement(1525095..1525523) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR545W 1 1526321 1531711 ON 4 YRF1-1 (1526321..1531711) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YDR545C-A 0 1530863 1531342 OFF 4 YDR545C-A complement(1530863..1531342) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-1/YDR545W YEL077C 0 264 4097 ON 5 YEL077C complement(264..4097) Helicase-like protein encoded within the telomeric Y' element YEL077W-A 1 630 1112 OFF 5 YEL077W-A (630..1112) Dubious open reading frame, unlike to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL076C-A 0 4185 5114 ON 5 YEL076C-A complement(4185..4322,4602..5114) Putative protein of unknown function YEL076C 0 4464 5114 ON 5 YEL076C complement(4464..5114) Putative protein of unknown function YEL075W-A 1 4870 5481 OFF 5 YEL075W-A (4870..5481) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL075C 0 5345 5713 ON 5 YEL075C complement(5345..5713) Putative protein of unknown function YEL074W 1 6126 6464 OFF 5 YEL074W (6126..6464) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large scale expression analysis showed an increase in mRNA accumulation in strains mutant for exosome components. YEL073C 0 7230 7553 ON 5 YEL073C complement(7230..7553) Putative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline YEL072W 1 13720 14415 ON 5 RMD6 (13720..14415) Protein required for sporulation YEL071W 1 16355 17845 ON 5 DLD3 (16355..17845) D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm YEL070W 1 19589 21097 ON 5 DSF1 (19589..21097) Deletion suppressor of mpt5 mutation YEL069C 0 21537 23231 ON 5 HXT13 complement(21537..23231) Hexose transporter, induced in the presence of non-fermentable carbon sources, induced by low levels of glucose, repressed by high levels of glucose YEL068C 0 25646 25978 OFF 5 YEL068C complement(25646..25978) Protein of unknown function; expressed at both mRNA and protein levels YEL067C 0 26189 26776 OFF 5 YEL067C complement(26189..26776) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YEL066W 1 26667 27206 ON 5 HPA3 (26667..27206) D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro YEL065W 1 27657 29543 ON 5 SIT1 (27657..29543) Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p YEL064C 0 29797 31239 ON 5 AVT2 complement(29797..31239) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YEL063C 0 31694 33466 ON 5 CAN1 complement(31694..33466) Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance YEL062W 1 34407 36254 ON 5 NPR2 (34407..36254) Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline YEL061C 0 36535 39537 ON 5 CIN8 complement(36535..39537) Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation YEL060C 0 40046 41953 ON 5 PRB1 complement(40046..41953) Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p YEL059C-A 0 42400 42624 ON 5 SOM1 complement(42400..42624) Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs YEL059W 1 42652 42960 OFF 5 HHY1 (42652..42960) Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking YEL058W 1 43252 44925 ON 5 PCM1 (43252..44925) Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors YEL057C 0 45020 45721 ON 5 YEL057C complement(45020..45721) Protein of unknown function involved in telomere maintenance; target of UME6 regulation YEL056W 1 47168 48373 ON 5 HAT2 (47168..48373) Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing YEL055C 0 48471 51539 ON 5 POL5 complement(48471..51539) DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Scer_snR80 0 52150 52320 ON 5 SNR80 complement(52150..52320) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U776 and small subunit (SSU) rRNA at position U759 YEL054C 0 52721 53218 ON 5 RPL12A complement(52721..53218) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins YEL053W-A 1 52908 53255 OFF 5 YEL053W-A (52908..53255) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YEL054C YEL053C 0 53901 56102 ON 5 MAK10 complement(53901..56102) Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible YEL052W 1 56571 58100 ON 5 AFG1 (56571..58100) Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain YEL051W 1 58378 59148 ON 5 VMA8 (58378..59148) Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis YEL050W-A 1 59549 59740 OFF 5 YEL050W-A (59549..59740) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YEL050C 0 59670 60851 ON 5 RML2 complement(59670..60851) Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Scer_snR67 1 61352 61433 ON 5 SNR67 (61352..61433) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at positions G2619 and U2724 Scer_snR53 1 61699 61789 ON 5 SNR53 (61699..61789) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position A796 Scer_5.trna1G 0 61890 61960 ON 5 tG-GCC complement(61890..61960) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YEL049W 1 63728 64090 ON 5 PAU2 (63728..64090) Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YEL048C 0 64709 65167 ON 5 TCA17 complement(64709..65167) Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder YEL047C 0 65385 66797 ON 5 YEL047C complement(65385..66797) Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies YEL046C 0 67629 68792 ON 5 GLY1 complement(67629..68792) Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis YEL045C 0 68840 69265 OFF 5 YEL045C complement(68840..69265) Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress YEL044W 1 69757 70257 ON 5 IES6 (69757..70257) Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes YEL043W 1 70478 73348 ON 5 YEL043W (70478..73348) Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold YEL042W 1 73771 75327 ON 5 GDA1 (73771..75327) Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate YEL041W 1 75944 77431 ON 5 YEF1 (75944..77431) ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes YEL040W 1 78053 79456 ON 5 UTR2 (78053..79456) Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck YEL039C 0 79636 79977 ON 5 CYC7 complement(79636..79977) Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration YEL038W 1 80462 81145 ON 5 UTR4 (80462..81145) Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus YEL037C 0 81407 82603 ON 5 RAD23 complement(81407..82603) Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover YEL036C 0 83050 84552 ON 5 ANP1 complement(83050..84552) Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol YEL035C 0 85045 85545 OFF 5 UTR5 complement(85045..85545) Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions YEL034C-A 0 85613 86215 OFF 5 YEL034C-A complement(85613..86215) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; completely overlaps HYP2/YEL034W, a verified gene that encodes eiF-5A YEL034W 1 85676 86149 ON 5 HYP2 (85676..86149) Translation elongation factor eIF-5A that may function in translation initiation; similar to and functionally redundant with Anb1p; structural homolog of bacterial EF-P; undergoes an essential hypusination modification YEL033W 1 86179 86598 OFF 5 MTC7 (86179..86598) Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Scer_5.trna2S 1 86604 86685 ON 5 tS-AGA (86604..86685) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YEL032W 1 86937 89852 ON 5 MCM3 (86937..89852) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex YEL032C-A 0 87040 87201 OFF 5 YEL032C-A complement(87040..87201) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL031W 1 90258 93905 ON 5 SPF1 (90258..93905) P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 YEL030C-A 0 94583 94897 OFF 5 YEL030C-A complement(94583..94897) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL030W 1 94644 96578 ON 5 ECM10 (94644..96578) Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations YEL029C 0 96858 97796 ON 5 BUD16 complement(96858..97796) Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) YEL028W 1 98668 99129 OFF 5 YEL028W (98668..99129) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Scer_5.trna3M 1 100133 100204 ON 5 tM-CAU (100133..100204) Methionine initiator tRNA (tRNA-Met), predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 74.96 YEL027W 1 100769 101251 ON 5 VMA3 (100769..101251) Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis YEL026W 1 101943 102323 ON 5 SNU13 (101943..102323) RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein YEL025C 0 102581 106147 ON 5 YEL025C complement(102581..106147) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YEL024W 1 107260 107907 ON 5 RIP1 (107260..107907) Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly YEL023C 0 108504 110552 ON 5 YEL023C complement(108504..110552) Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene YEL022W 1 111421 115800 ON 5 GEA2 (111421..115800) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p YEL021W 1 116167 116970 ON 5 URA3 (116167..116970) Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound YEL020C-B 0 117183 117380 OFF 5 YEL020C-B complement(117183..117380) Dubious open reading frame unlikely to encode a protein; partially overlaps verified gene YEL020W-A; identified by fungal homology and RT-PCR YEL020W-A 1 117211 117474 ON 5 TIM9 (117211..117474) Essential protein of the mitochondrial intermembrane space, forms a complex with Tim10p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Scer_RPR1 0 117667 118035 ON 5 RPR1 complement(117667..118035) RNA component of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; may be responsible for recognition of substrate tRNAs YEL020C 0 118617 120299 ON 5 YEL020C complement(118617..120299) Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data YEL019C 0 120498 121301 ON 5 MMS21 complement(120498..121301) SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination YEL018C-A 0 121455 121988 OFF 5 YEL018C-A complement(121455..121988) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL018W 1 121471 122310 ON 5 EAF5 (121471..122310) Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex YEL017C-A 0 122798 122929 ON 5 PMP2 complement(122798..122929) Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1 YEL017W 1 123657 124670 ON 5 GTT3 (123657..124670) Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery YEL016C 0 124737 126218 ON 5 NPP2 complement(124737..126218) Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation YEL015W 1 126629 128284 ON 5 EDC3 (126629..128284) Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies YEL014C 0 128303 128608 OFF 5 YEL014C complement(128303..128608) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL013W 1 128825 130561 ON 5 VAC8 (128825..130561) Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Scer_5.trna4Q 0 131082 131153 ON 5 tQ-UUG complement(131082..131153) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.78 YEL012W 1 131772 132551 ON 5 UBC8 (131772..131776,131900..132551) Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro YEL011W 1 133120 135234 ON 5 GLC3 (133120..135234) Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Scer_5.trna5K 0 135425 135497 ON 5 tK-CUU complement(135425..135497) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YEL010W 1 136279 136629 OFF 5 YEL010W (136279..136629) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL009C-A 0 136371 136778 OFF 5 YEL009C-A complement(136371..136778) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_5.trna6R 1 138666 138737 ON 5 tR-UCU (138666..138737) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YEL009C 0 138918 139763 ON 5 GCN4 complement(138918..139763) Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels YEL008W 1 140512 140892 OFF 5 YEL008W (140512..140892) Hypothetical protein predicted to be involved in metabolism YEL008C-A 0 140726 140818 OFF 5 YEL008C-A complement(140726..140818) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL007W 1 141892 143892 ON 5 MIT1 (141892..143892) Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 YEL006W 1 144327 145334 ON 5 YEA6 (144327..145334) Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog YEL005C 0 145907 146755 ON 5 VAB2 complement(145907..146755) Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter YEL004W 1 146951 147979 ON 5 YEA4 (146951..147979) Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER YEL003W 1 148176 148599 ON 5 GIM4 (148176..148194,148283..148599) Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it YEL002C 0 148722 150014 ON 5 WBP1 complement(148722..150014) Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum YEL001C 0 150301 150978 ON 5 IRC22 complement(150301..150978) Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci Scer_CEN5 1 151987 152104 ON 5 CEN5 (151987..152104) Chromosome V centromere YER001W 1 153520 155808 ON 5 MNN1 (153520..155808) Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family YER002W 1 156803 157498 ON 5 NOP16 (156803..157498) Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis YER003C 0 157736 159118 ON 5 PMI40 complement(157736..158994,159088..159118) Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation YER004W 1 159580 160275 ON 5 FMP52 (159580..160275) Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation YER005W 1 160550 162442 ON 5 YND1 (160550..162442) Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity YER006W 1 162723 164285 ON 5 NUG1 (162723..164285) GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus YER006C-A 0 164344 164661 OFF 5 YER006C-A complement(164344..164661) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PAC2 YER007W 1 164527 166083 ON 5 PAC2 (164527..166083) Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl YER007C-A 0 166237 166885 ON 5 TMA20 complement(166237..166771,166875..166885) Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1 Scer_snR14 0 167427 167586 ON 5 SNR14 complement(167427..167586) U4 spliceosomal RNA (U4 snRNA); interacts with U6 YER008C 0 167808 171818 ON 5 SEC3 complement(167808..171818) Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion YER009W 1 172115 172492 ON 5 NTF2 (172115..172492) Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport YER010C 0 172634 173338 ON 5 YER010C complement(172634..173338) Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction YER011W 1 175248 176012 ON 5 TIR1 (175248..176012) Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Scer_5.trna7E 1 177099 177170 ON 5 tE-UUC (177099..177170) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YER012W 1 177835 178431 ON 5 PRE1 (177835..178431) Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle YER013W 1 178841 182278 ON 5 PRP22 (178841..182278) DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes YER014W 1 182600 184219 ON 5 HEM14 (182600..184219) Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides YER014C-A 0 183731 184700 OFF 5 BUD25 complement(183731..184169,184678..184700) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YER015W 1 184541 186775 ON 5 FAA2 (184541..186775) Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome YER016W 1 188277 189311 ON 5 BIM1 (188277..189311) Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally YER017C 0 189503 191788 ON 5 AFG3 complement(189503..191788) Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes YER018C 0 191959 192624 ON 5 SPC25 complement(191959..192624) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering YER019W 1 192797 194230 ON 5 ISC1 (192797..194230) Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance YER019C-A 0 194273 194539 ON 5 SBH2 complement(194273..194539) Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p YER020W 1 195168 196517 ON 5 GPA2 (195168..196517) Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YER021W 1 196948 198519 ON 5 RPN3 (196948..198519) Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control YER022W 1 198812 200875 ON 5 SRB4 (198812..200875) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination YER023W 1 201076 201936 ON 5 PRO3 (201076..201936) Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis YER023C-A 0 201522 201734 OFF 5 YER023C-A complement(201522..201734) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene PRO3; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YER024W 1 202192 204963 ON 5 YAT2 (202192..204963) Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane YER025W 1 205251 206834 ON 5 GCD11 (205251..206834) Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Scer_5.trna8H 1 207357 207428 ON 5 tH-GUG (207357..207428) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YER026C 0 207644 208474 ON 5 CHO1 complement(207644..208474) Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline YER027C 0 208979 210232 ON 5 GAL83 complement(208979..210232) One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain YER028C 0 210692 211876 ON 5 MIG3 complement(210692..211876) Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes YER029C 0 212587 213177 ON 5 SMB1 complement(212587..213177) Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B' YER030W 1 213437 213898 ON 5 CHZ1 (213437..213898) Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif YER031C 0 214076 214747 ON 5 YPT31 complement(214076..214747) Rab family GTPase, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi YER032W 1 215063 217693 ON 5 FIR1 (215063..217693) Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate YER033C 0 218057 221287 ON 5 ZRG8 complement(218057..221287) Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency YER034W 1 221846 222403 ON 5 YER034W (221846..222403) Putative protein of unknown function; non-essential gene; expression induced upon calcium shortage YER035W 1 222639 223076 ON 5 EDC2 (222639..223076) RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress YER036C 0 223367 225199 ON 5 ARB1 complement(223367..225199) ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p YER037W 1 225889 226854 ON 5 PHM8 (225889..226854) Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p YER038C 0 226858 228252 ON 5 KRE29 complement(226858..228252) Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance YER038W-A 1 228451 228831 OFF 5 YER038W-A (228451..228831) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria YER039C 0 228456 229205 ON 5 HVG1 complement(228456..229205) Protein of unknown function, has homology to Vrg4p YER039C-A 0 229263 229481 OFF 5 YER039C-A complement(229263..229481) Putative protein of unknown function; YER039C-A is not an essential gene YER040W 1 229795 231987 ON 5 GLN3 (229795..231987) Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source YER041W 1 232461 234740 ON 5 YEN1 (232461..234740) Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p YER042W 1 234937 235491 ON 5 MXR1 (234937..235491) Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan YER043C 0 235770 237119 ON 5 SAH1 complement(235770..237119) S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor YER044C 0 237570 238016 ON 5 ERG28 complement(237570..238016) Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p YER044C-A 0 238460 239774 ON 5 MEI4 complement(238460..239623,239712..239774) Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores YER045C 0 240032 241501 ON 5 ACA1 complement(240032..241501) Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, may regulate transcription of genes involved in utilization of non-optimal carbon sources YER046W 1 243180 243611 ON 5 SPO73 (243180..243611) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis YER046W-A 1 243700 244029 OFF 5 YER046W-A (243700..244029) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER047C 0 243810 246503 ON 5 SAP1 complement(243810..246503) Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system YER048C 0 246982 248157 ON 5 CAJ1 complement(246982..248157) Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Scer_5.trna9Q 1 250286 250357 ON 5 tQ-UUG (250286..250357) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YER048W-A 1 250718 251002 ON 5 ISD11 (250718..251002) Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis YER049W 1 251728 253662 ON 5 TPA1 (251728..253662) Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate YER050C 0 253971 254387 ON 5 RSM18 complement(253971..254387) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein YER051W 1 254656 256134 ON 5 JHD1 (254656..256134) JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe YER052C 0 256375 257958 ON 5 HOM3 complement(256375..257958) Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis YER053C 0 258737 259639 ON 5 PIC2 complement(258737..259639) Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature YER053C-A 0 260933 261046 ON 5 YER053C-A complement(260933..261046) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YER054C 0 262052 263698 ON 5 GIP2 complement(262052..263698) Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p YER055C 0 264892 265785 ON 5 HIS1 complement(264892..265785) ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control YER056C 0 266512 268113 ON 5 FCY2 complement(266512..268113) Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation YER056C-A 0 269423 270185 ON 5 RPL34A complement(269423..269751,270149..270185) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein YER057C 0 270737 271126 ON 5 HMF1 complement(270737..271126) Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro YER058W 1 271768 272091 ON 5 PET117 (271768..272091) Protein required for assembly of cytochrome c oxidase YER059W 1 272624 273886 ON 5 PCL6 (272624..273886) Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding YER060W 1 274567 276153 ON 5 FCY21 (274567..276153) Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function YER060W-A 1 276572 278164 ON 5 FCY22 (276572..278164) Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function YER061C 0 278298 279626 ON 5 CEM1 complement(278298..279626) Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration YER062C 0 279930 280682 ON 5 HOR2 complement(279930..280682) One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition YER063W 1 281710 282366 ON 5 THO1 (281710..282366) Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation YER064C 0 282705 284222 ON 5 VHR2 complement(282705..284222) Non-essential nuclear protein; null mutation has global effects on transcription YER065C 0 285241 286914 ON 5 ICL1 complement(285241..286914) Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Scer_5.trna10S 0 288443 288524 ON 5 tS-UGA complement(288443..288524) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Ser (S) | Anti-Codon: UGA | Cove Score: 84.16 YER066W 1 290242 290799 ON 5 RRT13 (290242..290799) Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription YER066C-A 0 291703 292203 OFF 5 YER066C-A complement(291703..292203) Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W YER067W 1 292066 292551 ON 5 RGI1 (292066..292551) Protein of unknown function involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion YER067C-A 0 292240 292563 OFF 5 YER067C-A complement(292240..292563) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W YER068W 1 293050 294813 ON 5 MOT2 (293050..294813) Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications YER068C-A 0 295301 295732 OFF 5 YER068C-A complement(295301..295732) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER069W 1 295410 298001 ON 5 ARG5,6 (295410..298001) Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine YER070W 1 298950 301616 ON 5 RNR1 (298950..301616) Major isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits YER071C 0 301947 302327 ON 5 TDA2 complement(301947..302327) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele YER072W 1 302806 303195 ON 5 VTC1 (302806..303195) Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity YER073W 1 304030 305592 ON 5 ALD5 (304030..305592) Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed YER074W 1 306323 307196 ON 5 RPS24A (306323..306325,306792..307196) Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein YER074W-A 1 307653 308123 ON 5 YOS1 (307653..307746,307849..307956,308068..308123) Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p YER075C 0 308413 311199 ON 5 PTP3 complement(308413..311199) Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Scer_5.trna11A 0 312023 312095 ON 5 tA-UGC complement(312023..312095) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: UGC | Cove Score: 76.57 YER076C 0 312590 313498 ON 5 YER076C complement(312590..313498) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization YER076W-A 1 313390 313737 OFF 5 YER076W-A (313390..313737) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER076C YER077C 0 314534 316600 ON 5 YER077C complement(314534..316600) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport YER078C 0 316807 318342 ON 5 ICP55 complement(316807..318342) Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family YER078W-A 1 318646 318810 OFF 5 YER078W-A (318646..318810) Putative protein of unknown function; identified by fungal homology and RT-PCR YER079W 1 318920 319552 ON 5 YER079W (318920..319552) Putative protein of unknown function YER079C-A 0 319902 320240 OFF 5 YER079C-A complement(319902..320240) Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER080W YER080W 1 319963 321846 ON 5 AIM9 (319963..321846) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Scer_SRG1 1 322212 322762 ON 5 SRG1 (322212..322762) Non-protein-coding RNA that regulates the transcription of SER3; expression of SRG1 RNA represses expression of its neighboring gene SER3 via a transcription-interference mechanism YER081W 1 322686 324095 ON 5 SER3 (322686..324095) 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p YER082C 0 324272 325936 ON 5 UTP7 complement(324272..325936) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YER083C 0 326174 327031 ON 5 GET2 complement(326174..327031) Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division YER084W 1 327065 327451 OFF 5 YER084W (327065..327451) Protein of unknown function; expressed at both mRNA and protein levels YER084W-A 1 327598 328110 OFF 5 YER084W-A (327598..328110) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER085C 0 327619 328140 ON 5 YER085C complement(327619..328140) Putative protein of unknown function YER086W 1 328477 330207 ON 5 ILV1 (328477..330207) Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation YER087W 1 330576 332306 ON 5 AIM10 (330576..332306) Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss YER087C-A 0 331817 332368 OFF 5 YER087C-A complement(331817..332368) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase YER087C-B 0 332582 332830 ON 5 SBH1 complement(332582..332830) Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p YER088C 0 333176 335188 ON 5 DOT6 complement(333176..335188) Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; similar to Tod6p; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation YER088C-A 0 335696 336019 OFF 5 YER088C-A complement(335696..336019) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER088W-B 1 335927 336073 OFF 5 YER088W-B (335927..336073) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the ORF PTC2/YER089C; identified by gene-trapping, expression analysis, and homology searching YER089C 0 335946 337340 ON 5 PTC2 complement(335946..337340) Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint YER090W 1 337949 339472 ON 5 TRP2 (337949..339472) Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p YER090C-A 0 338322 338411 OFF 5 YER090C-A complement(338322..338411) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER091C 0 339864 342167 ON 5 MET6 complement(339864..342167) Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs YER091C-A 0 342390 342611 OFF 5 YER091C-A complement(342390..342611) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YER092W 1 342855 343232 ON 5 IES5 (342855..343232) Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions YER093C 0 343320 347612 ON 5 TSC11 complement(343320..347612) Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain YER093C-A 0 347912 348400 ON 5 AIM11 complement(347912..348201,348277..348400) Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron YER094C 0 348729 349346 ON 5 PUP3 complement(348729..349346) Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 YER095W 1 349980 351182 ON 5 RAD51 (349980..351182) Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein YER096W 1 351698 353236 ON 5 SHC1 (351698..353236) Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH Scer_5.trna12E 1 354934 355005 ON 5 tE-UUC (354934..355005) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YER097W 1 355140 355469 OFF 5 YER097W (355140..355469) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER098W 1 355466 357730 ON 5 UBP9 (355466..357730) Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions YER099C 0 358105 359061 ON 5 PRS2 complement(358105..359061) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YER100W 1 359562 360314 ON 5 UBC6 (359562..360314) Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway YER101C 0 360502 361794 ON 5 AST2 complement(360502..361794) Similar to lipid raft associated protein Ast1p; similarly to Ast1p, overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily YER102W 1 363100 363702 ON 5 RPS8B (363100..363702) Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein YER103W 1 364589 366517 ON 5 SSA4 (364589..366517) Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation YER104W 1 366802 367428 ON 5 RTT105 (366802..367428) Protein with a role in regulation of Ty1 transposition YER105C 0 367838 372013 ON 5 NUP157 complement(367838..372013) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; has similarity to Nup170p; both Nup170p and NUP157p are similar to human Nup155p YER106W 1 372326 373234 ON 5 MAM1 (372326..373234) Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle YER107C 0 373448 374545 ON 5 GLE2 complement(373448..374545) RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 YER107W-A 1 374394 374714 OFF 5 YER107W-A (374394..374714) Dubious open reading frame unlikely to encode a protein, partially overlaps verified ORF GLE2/YER107C YER109C 0 375215 377614 ON 5 FLO8 complement(375215..377614) Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene YER110C 0 378762 382103 ON 5 KAP123 complement(378762..382103) Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 YER111C 0 382595 385876 ON 5 SWI4 complement(382595..385876) DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair YER112W 1 387232 387795 ON 5 LSM4 (387232..387795) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YER113C 0 387932 390052 ON 5 TMN3 complement(387932..390052) Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation YER114C 0 390590 393712 ON 5 BOI2 complement(390590..393712) Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain YER115C 0 394292 394867 ON 5 SPR6 complement(394292..394867) Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation YER116C 0 395348 396172 ON 5 SLX8 complement(395348..396172) Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain YER117W 1 396769 397653 ON 5 RPL23B (396769..396810,397282..397653) Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli L14 and rat L23 ribosomal proteins YER118C 0 397952 399055 ON 5 SHO1 complement(397952..399055) Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p YER119C 0 399496 400842 ON 5 AVT6 complement(399496..400842) Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation YER119C-A 0 400867 401238 OFF 5 YER119C-A complement(400867..401238) Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 YER120W 1 401135 401869 ON 5 SCS2 (401135..401869) Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog YER121W 1 402375 402719 OFF 5 YER121W (402375..402719) Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy YER122C 0 402871 404352 ON 5 GLO3 complement(402871..404352) ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p YER123W 1 404813 406387 ON 5 YCK3 (404813..406387) Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway YER124C 0 407342 409063 ON 5 DSE1 complement(407342..409063) Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall YER125W 1 410189 412618 ON 5 RSP5 (410189..412618) E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p YER126C 0 413394 414179 ON 5 NSA2 complement(413394..414179) Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA YER127W 1 414481 415554 ON 5 LCP5 (414481..415554) Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus YER128W 1 415859 416470 ON 5 VFA1 (415859..416470) Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology YER129W 1 417281 420709 ON 5 SAK1 (417281..420709) Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome YER130C 0 421115 422446 ON 5 YER130C complement(421115..422446) Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress YER131W 1 423952 424311 ON 5 RPS26B (423952..424311) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein Scer_snR4 1 424698 424883 ON 5 SNR4 (424698..424883) C/D box small nucleolar RNA (snoRNA) but not shown to be active and no target site is predicted within the ribosomal RNAs YER132C 0 425188 430449 ON 5 PMD1 complement(425188..430449) Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Scer_snR52 0 431129 431220 ON 5 SNR52 complement(431129..431220) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U2921 and small subunit (SSU) rRNA at position A420 YER133W 1 432495 433958 ON 5 GLC7 (432495..432671,433197..433958) Type 1 serine/threonine protein phosphatase catalytic subunit, involved in many processes (eg: glycogen metabolism, sporulation, mitosis); accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits Scer_5.trna13H 0 434541 434612 ON 5 tH-GUG complement(434541..434612) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 Scer_5.trna14K 0 435752 435824 ON 5 tK-CUU complement(435752..435824) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YER133W-A 1 437191 437532 OFF 5 YER133W-A (437191..437532) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YER134C. YER134C 0 437267 437803 ON 5 YER134C complement(437267..437803) Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene YER135C 0 437952 438344 OFF 5 YER135C complement(437952..438344) Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Scer_5.trna15V 1 438700 438773 ON 5 tV-AAC (438700..438773) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YER136W 1 439616 440971 ON 5 GDI1 (439616..440971) GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins YER137C 0 441373 441819 ON 5 YER137C complement(441373..441819) Putative protein of unknown function YER137W-A 1 441575 441880 OFF 5 YER137W-A (441575..441880) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_SCR1 1 441987 442508 ON 5 SCR1 (441987..442508) RNA subunit of the Signal Recognition Particle (SRP) Scer_5.trna16I 0 443202 443275 ON 5 tI-AAU complement(443202..443275) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YER138W-A 1 449474 449578 OFF 5 YER138W-A (449474..449578) Putative protein of unknown function YER139C 0 450563 451243 ON 5 RTR1 complement(450563..451243) CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm YER140W 1 451565 453235 ON 5 YER140W (451565..453235) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; genetic interactions suggest a role in folding of ER membrane proteins YER141W 1 453459 454919 ON 5 COX15 (453459..454919) Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase YER142C 0 455146 456036 ON 5 MAG1 complement(455146..456036) 3-methyl-adenine DNA glycosylase involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired YER143W 1 456319 457605 ON 5 DDI1 (456319..457605) DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains YER144C 0 457806 460223 ON 5 UBP5 complement(457806..460223) Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck YER145C 0 460526 461740 ON 5 FTR1 complement(460526..461740) High affinity iron permease involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron YER145C-A 0 462386 462823 OFF 5 YER145C-A complement(462386..462823) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF LSM5/YER146W YER146W 1 462585 462866 ON 5 LSM5 (462585..462866) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YER147C 0 462968 464842 ON 5 SCC4 complement(462968..464842) Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX YER147C-A 0 465203 465613 OFF 5 YER147C-A complement(465203..465613) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER148W 1 465303 466025 ON 5 SPT15 (465303..466025) TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability YER148W-A 1 466185 466763 OFF 5 YER148W-A (466185..466763) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER149C 0 466208 467470 ON 5 PEA2 complement(466208..467470) Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth YER150W 1 468370 468816 ON 5 SPI1 (468370..468816) GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Scer_5.trna17V 1 469457 469530 ON 5 tV-AAC (469457..469530) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YER151C 0 469686 472424 ON 5 UBP3 complement(469686..472424) Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity YER152C 0 472657 473988 ON 5 YER152C complement(472657..473988) Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene YER152W-A 1 473485 474051 OFF 5 YER152W-A (473485..474051) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YER153C 0 474041 474805 ON 5 PET122 complement(474041..474805) Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane YER154W 1 475020 476228 ON 5 OXA1 (475020..476228) Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals YER155C 0 476345 482848 ON 5 BEM2 complement(476345..482848) Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence YER156C 0 483325 484341 ON 5 YER156C complement(483325..484341) Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 YER157W 1 484788 487193 ON 5 COG3 (484788..487193) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Scer_5.trna18E 0 487331 487402 ON 5 tE-UUC complement(487331..487402) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YER158C 0 488857 490578 ON 5 YER158C complement(488857..490578) Protein of unknown function, has similarity to Afr1p; potentially phosphorylated by Cdc28p YER158W-A 1 491487 491702 OFF 5 YER158W-A (491487..491702) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER159C 0 491530 491958 ON 5 BUR6 complement(491530..491958) Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Scer_5.trna19R 0 492352 492424 ON 5 tR-ACG complement(492352..492424) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 71.46 YER161C 0 499347 500348 ON 5 SPT2 complement(499347..500348) Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins YER162C 0 500630 502894 ON 5 RAD4 complement(500630..502894) Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins YER163C 0 503084 503782 ON 5 YER163C complement(503084..503782) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle; weak similarity to bacterial cation transport protein YER164W 1 505392 509798 ON 5 CHD1 (505392..509798) Chromatin remodeler that regulates various aspects of transcription; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes YER165W 1 510373 512106 ON 5 PAB1 (510373..512106) Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G YER165C-A 0 512627 512983 OFF 5 YER165C-A complement(512627..512983) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DNF1/YER166W YER166W 1 512744 517459 ON 5 DNF1 (512744..517459) Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase YER167W 1 518216 520771 ON 5 BCK2 (518216..520771) Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations YER168C 0 521029 522669 ON 5 CCA1 complement(521029..522669) ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites YER169W 1 523369 525759 ON 5 RPH1 (523369..525759) JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway YER170W 1 525974 526651 ON 5 ADK2 (525974..526651) Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background YER171W 1 527082 529418 ON 5 RAD3 (527082..529418) 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance YER172C 0 529530 536021 ON 5 BRR2 complement(529530..536021) RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD YER172C-A 0 536275 536655 OFF 5 YER172C-A complement(536275..536655) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER173W 1 536300 538279 ON 5 RAD24 (536300..538279) Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein YER174C 0 538434 539168 ON 5 GRX4 complement(538434..539168) Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 YER175C 0 539464 540363 ON 5 TMT1 complement(539464..540363) Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle YER175W-A 1 540650 540814 OFF 5 YER175W-A (540650..540814) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER176W 1 541690 545055 ON 5 ECM32 (541690..545055) DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes YER177W 1 545611 546414 ON 5 BMH1 (545611..546414) 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling YER178W 1 546817 548079 ON 5 PDA1 (546817..548079) E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose YER179W 1 548421 549517 ON 5 DMC1 (548421..548552,548645..549517) Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p YER180C 0 549724 550527 ON 5 ISC10 complement(549724..550527) Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells YER180C-A 0 550865 551122 ON 5 SLO1 complement(550865..551122) Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Scer_5.trna20I 0 551285 551358 ON 5 tI-AAU complement(551285..551358) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YER181C 0 551473 551796 OFF 5 YER181C complement(551473..551796) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies YER182W 1 552525 553259 ON 5 FMP10 (552525..553259) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YER183C 0 553334 553969 ON 5 FAU1 complement(553334..553969) 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis YER184C 0 556296 558680 ON 5 YER184C complement(556296..558680) Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source YER185W 1 559454 560365 ON 5 PUG1 (559454..560365) Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins YER186C 0 561705 562625 ON 5 YER186C complement(561705..562625) Putative protein of unknown function YER187W 1 566230 566655 ON 5 YER187W (566230..566655) Putative protein of unknown function; induced in respiratory-deficient cells YER188W 1 568040 568759 OFF 5 YER188W (568040..568759) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p YER188C-A 0 569608 569907 OFF 5 YER188C-A complement(569608..569907) Putative protein of unknown function YER189W 1 571155 571523 ON 5 YER189W (571155..571523) Putative protein of unknown function YER190W 1 571480 576525 ON 5 YRF1-2 (571480..576525) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p; induced by treatment with 8-methoxypsoralen and UVA irradiation YER190C-A 0 574804 575379 OFF 5 YER190C-A complement(574804..575379) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YRF1-2/YER190W; identified by gene-trapping, expression analysis, and homology YER190C-B 0 575680 576162 OFF 5 YER190C-B complement(575680..576162) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFL068W 1 53 535 ON 6 YFL068W (53..535) Putative protein of unknown function YFL067W 1 836 1363 OFF 6 YFL067W (836..1363) Protein of unknown function, down-regulated at low calcium levels YFL066C 0 1437 2615 ON 6 YFL066C complement(1437..2615) Helicase-like protein encoded within the telomeric Y' element; induced by treatment with 8-methoxypsoralen and UVA irradiation YFL065C 0 3030 3338 ON 6 YFL065C complement(3030..3338) Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation YFL064C 0 3322 3846 ON 6 YFL064C complement(3322..3846) Putative protein of unknown function YFL063W 1 5066 5521 OFF 6 YFL063W (5066..5521) Dubious open reading frame, based on available experimental and comparative sequence data YFL062W 1 6426 7565 ON 6 COS4 (6426..7565) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YFL061W 1 9545 10222 ON 6 DDI2 (9545..10222) Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants YFL060C 0 10301 10969 ON 6 SNO3 complement(10301..10969) Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin YFL059W 1 11363 12259 ON 6 SNZ3 (11363..12259) Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3 YFL058W 1 12929 13951 ON 6 THI5 (12929..13951) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YFL057C 0 14305 14763 ON 6 AAD16 complement(14305..14763) Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YFL056C 0 14793 15431 ON 6 AAD6 complement(14793..15431) Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin YFL055W 1 17004 18680 ON 6 AGP3 (17004..18680) Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition YFL054C 0 20847 22787 ON 6 YFL054C complement(20847..22787) Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol YFL053W 1 23423 25198 ON 6 DAK2 (23423..25198) Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation YFL052W 1 28232 29629 ON 6 YFL052W (28232..29629) Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity YFL051C 0 30058 30540 ON 6 YFL051C complement(30058..30540) Putative protein of unknown function; YFL051C is not an essential gene YFL050C 0 33272 35848 ON 6 ALR2 complement(33272..35848) Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition YFL049W 1 36803 38674 ON 6 SWP82 (36803..38674) Member of the SWI/SNF chromatin remodeling complex in which it plays an as yet unidentified role; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p YFL048C 0 38843 40180 ON 6 EMP47 complement(38843..40180) Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport YFL047W 1 40421 42565 ON 6 RGD2 (40421..42565) GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p YFL046W 1 42815 43438 ON 6 FMP32 (42815..43438) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YFL045C 0 43628 44392 ON 6 SEC53 complement(43628..44392) Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen YFL044C 0 44655 45560 ON 6 OTU1 complement(44655..45560) Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family YFL042C 0 45720 47744 ON 6 YFL042C complement(45720..47744) Putative protein of unknown function; YFL042C is not an essential gene YFL041W-A 1 48734 48925 OFF 6 YFL041W-A (48734..48925) Putative protein of unknown function; identified by fungal homology and RT-PCR YFL041W 1 49139 51007 ON 6 FET5 (49139..51007) Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport YFL040W 1 51350 52972 ON 6 YFL040W (51350..52972) Putative transporter, member of the sugar porter family; YFL040W is not an essential gene YFL039C 0 53260 54696 ON 6 ACT1 complement(53260..54377,54687..54696) Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions YFL038C 0 55366 55986 ON 6 YPT1 complement(55366..55986) Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) YFL037W 1 56336 57709 ON 6 TUB2 (56336..57709) Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules Scer_RUF21 0 57815 58521 ON 6 RUF21 complement(57815..58521) RNA of Unknown Function YFL036W 1 58782 62837 ON 6 RPO41 (58782..62837) Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation YFL034C-B 0 63016 63993 ON 6 MOB2 complement(63016..63859,63974..63993) Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p YFL034C-A 0 64243 64932 ON 6 RPL22B complement(64243..64599,64921..64932) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein YFL034W 1 65477 68698 ON 6 YFL034W (65477..68698) Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk YFL033C 0 69115 74427 ON 6 RIM15 complement(69115..74427) Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase YFL032W 1 74872 75192 OFF 6 YFL032W (74872..75192) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene YFL031W 1 75179 76147 ON 6 HAC1 (75179..75839,76092..76147) Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog) that regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis YFL031C-A 0 76014 76115 OFF 6 YFL031C-A complement(76014..76115) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps HAC1/YFL031W; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFL030W 1 76831 77988 ON 6 AGX1 (76831..77988) Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases YFL029C 0 78055 79161 ON 6 CAK1 complement(78055..79161) Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases YFL028C 0 79344 80213 ON 6 CAF16 complement(79344..80213) Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator YFL027C 0 80419 81912 ON 6 GYP8 complement(80419..81912) GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport YFL026W 1 82580 83875 ON 6 STE2 (82580..83875) Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells YFL025C 0 84145 87234 ON 6 BST1 complement(84145..87234) GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules YFL024C 0 87847 90345 ON 6 EPL1 complement(87847..90345) Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); homologous to Drosophila Enhancer of Polycomb YFL023W 1 90986 93376 ON 6 BUD27 (90986..93376) Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding YFL022C 0 93499 95010 ON 6 FRS2 complement(93499..95010) Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar YFL021C-A 0 95761 96615 OFF 6 YFL021C-A complement(95761..96615) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFL021W 1 95966 97498 ON 6 GAT1 (95966..97498) Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p YFL020C 0 99231 99599 ON 6 PAU5 complement(99231..99599) Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme YFL019C 0 100252 100605 OFF 6 YFL019C complement(100252..100605) Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Scer_6.trna1P 1 101376 101478 ON 6 tP-UGG (101376..101411,101443..101478) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 60.63 YFL018C 0 101628 103127 ON 6 LPD1 complement(101628..103127) Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes YFL017W-A 1 103699 103932 ON 6 SMX2 (103699..103932) Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G YFL017C 0 103983 104462 ON 6 GNA1 complement(103983..104462) Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA YFL016C 0 104701 106236 ON 6 MDJ1 complement(104701..106236) Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones YFL015W-A 1 106415 106732 OFF 6 YFL015W-A (106415..106732) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFL015C 0 106469 106963 OFF 6 YFL015C complement(106469..106963) Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene YFL014W 1 107256 107585 ON 6 HSP12 (107256..107585) Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; regulated by HOG and Ras-Pka pathways YFL013W-A 1 107799 108602 OFF 6 YFL013W-A (107799..108602) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YFL013C 0 107852 109930 ON 6 IES1 complement(107852..109930) Subunit of the INO80 chromatin remodeling complex YFL012W-A 1 109810 110184 OFF 6 YFL012W-A (109810..110184) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene IES1/YFL013C YFL012W 1 110647 111093 ON 6 YFL012W (110647..111093) Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin YFL011W 1 112345 113985 ON 6 HXT10 (112345..113985) Putative hexose transporter, expressed at low levels and expression is repressed by glucose YFL010W-A 1 114990 115274 OFF 6 AUA1 (114990..115274) Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease YFL010C 0 115108 115743 ON 6 WWM1 complement(115108..115743) WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 YFL009W 1 116145 118484 ON 6 CDC4 (116145..118484) F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p YFL008W 1 119429 123106 ON 6 SMC1 (119429..123106) Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure YFL007W 1 123479 129910 ON 6 BLM10 (123479..129910) Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 YFL005W 1 130334 130981 ON 6 SEC4 (130334..130981) Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Scer_RUF20 0 131061 131503 ON 6 RUF20 complement(131061..131503) RNA of Unknown Function YFL004W 1 131810 134296 ON 6 VTC2 (131810..134296) Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion YFL003C 0 134521 137157 ON 6 MSH4 complement(134521..137157) Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein Scer_6.trna2N 0 137486 137559 ON 6 tN-GUU complement(137486..137559) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YFL002C 0 145114 146934 ON 6 SPB4 complement(145114..146934) Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients YFL001W 1 147131 148459 ON 6 DEG1 (147131..148459) tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Scer_CEN6 1 148510 148627 ON 6 CEN6 (148510..148627) Chromosome VI centromere YFR001W 1 149110 149724 ON 6 LOC1 (149110..149724) Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles YFR002W 1 150016 152535 ON 6 NIC96 (150016..152535) Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) YFR003C 0 152657 153124 ON 6 YPI1 complement(152657..153124) Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance YFR004W 1 153393 154313 ON 6 RPN11 (153393..154313) Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes YFR005C 0 154527 155873 ON 6 SAD1 complement(154527..155873) Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle YFR006W 1 156145 157752 ON 6 YFR006W (156145..157752) Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Scer_6.trna3F 0 157916 158007 ON 6 tF-GAA complement(157916..157951,157971..158007) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YFR007W 1 159299 160360 ON 6 YFH7 (159299..160360) Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases YFR008W 1 160535 161200 ON 6 FAR7 (160535..161200) Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p Scer_6.trna4G 1 162228 162298 ON 6 tG-GCC (162228..162298) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YFR009W 1 162488 164746 ON 6 GCN20 (162488..164746) Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA YFR009W-A 1 163875 164132 OFF 6 YFR009W-A (163875..164132) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YFR009W; identified by expression profiling and mass spectrometry YFR010W 1 165067 166566 ON 6 UBP6 (165067..166566) Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance YFR010W-A 1 166728 166916 OFF 6 YFR010W-A (166728..166916) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YFR011C; identified by expression profiling and mass spectrometry YFR011C 0 166746 167258 ON 6 AIM13 complement(166746..167258) Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Scer_6.trna5Y 1 167437 167525 ON 6 tY-GUA (167437..167475,167490..167525) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.18 YFR012W 1 167888 168496 ON 6 DCV1 (167888..168496) Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1 YFR012W-A 1 169224 169310 OFF 6 YFR012W-A (169224..169310) Putative protein of unknown function; identified by homology YFR013W 1 169922 172285 ON 6 IOC3 (169922..172285) Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing YFR014C 0 172537 173877 ON 6 CMK1 complement(172537..173877) Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian Cam Kinase II YFR015C 0 174265 176391 ON 6 GSY1 complement(174265..176391) Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase YFR016C 0 177042 180743 ON 6 YFR016C complement(177042..180743) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene Scer_6.trna6G 0 180974 181044 ON 6 tG-GCC complement(180974..181044) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YFR017C 0 182274 182861 ON 6 IGD1 complement(182274..182861) Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation YFR018C 0 183131 184222 ON 6 YFR018C complement(183131..184222) Putative protein of unknown function YFR019W 1 184502 191338 ON 6 FAB1 (184502..191338) 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Scer_6.trna7S 0 191513 191613 ON 6 tS-GCU complement(191513..191557,191577..191613) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: GCU | Cove Score: 68.67 YFR020W 1 192737 193435 ON 6 YFR020W (192737..193435) Protein of unknown function; mRNA identified as translated by ribosome profiling data YFR021W 1 194812 196314 ON 6 ATG18 (194812..196314) Phosphoinositide binding protein required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein YFR022W 1 196833 199034 ON 6 ROG3 (196833..199034) Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; has similarity to Rod1p; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins Scer_RUF22 0 199299 199813 ON 6 RUF22 complement(199299..199813) RNA of Unknown Function; precise location of 3' end uncertain; RUF22 may be longer than currently annotated YFR023W 1 199874 201709 ON 6 PES4 (199874..201709) Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation, similar to Mip6p YFR024C-A 0 201960 203433 ON 6 LSB3 complement(201960..203292,203387..203433) Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YFR025C 0 203743 204750 ON 6 HIS2 complement(203743..204750) Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Scer_6.trna8A 0 204924 204996 ON 6 tA-AGC complement(204924..204996) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YFR026C 0 205748 206257 ON 6 ULI1 complement(205748..206257) Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response YFR027W 1 207452 208297 ON 6 ECO1 (207452..208297) Acetyltransferase required for establishment of sister chromatid cohesion; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome YFR028C 0 208413 210068 ON 6 CDC14 complement(208413..210068) Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Scer_6.trna9Y 0 210619 210707 ON 6 tY-GUA complement(210619..210654,210669..210707) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.18 YFR029W 1 210937 212973 ON 6 PTR3 (210937..212973) Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes YFR030W 1 213312 216419 ON 6 MET10 (213312..216419) Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide YFR031C 0 216594 220106 ON 6 SMC2 complement(216594..220106) Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus YFR031C-A 0 220507 221418 ON 6 RPL2A complement(220507..221267,221415..221418) Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins Scer_RUF23 1 221714 221967 ON 6 RUF23 (221714..221967) RNA of Unknown Function; precise location of 3' end uncertain; RUF23 may be longer than currently annotated YFR032C 0 222090 222959 ON 6 RRT5 complement(222090..222959) Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation YFR032C-A 0 223258 223437 ON 6 RPL29 complement(223258..223437) Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein; not essential for translation, but required for proper joining of the large and small ribosomal subunits and for normal translation rate YFR032C-B 0 223710 223973 OFF 6 YFR032C-B complement(223710..223973) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFR033C 0 224326 224769 ON 6 QCR6 complement(224326..224769) Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1 YFR034C 0 225020 225958 ON 6 PHO4 complement(225020..225958) Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability YFR034W-A 1 226022 226309 OFF 6 YFR034W-A (226022..226309) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YFR035C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YFR035C 0 226121 226465 OFF 6 YFR035C complement(226121..226465) Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Scer_YGOB_ADL119W 1 226260 226550 ON 6 ADL119W (226260..226550) ANNOTATED BY YGOB - Scer_6.trna10K 0 226688 226760 ON 6 tK-CUU complement(226688..226760) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 70.32 YFR036W 1 226963 227337 ON 6 CDC26 (226963..227337) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YFR036W-A 1 227451 228101 OFF 6 YFR036W-A (227451..228101) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RSC8 YFR037C 0 227513 229186 ON 6 RSC8 complement(227513..229186) Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters YFR038W 1 229380 231941 ON 6 IRC5 (229380..231941) Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci YFR039C 0 232012 233544 ON 6 YFR039C complement(232012..233544) Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces YFR040W 1 234242 237250 ON 6 SAP155 (234242..237250) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p YFR041C 0 237368 238255 ON 6 ERJ5 complement(237368..238255) Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response YFR042W 1 238458 239060 ON 6 KEG1 (238458..239060) Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability YFR043C 0 239114 239827 ON 6 IRC6 complement(239114..239827) Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci YFR044C 0 239992 241437 ON 6 DUG1 complement(239992..241437) Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) YFR045W 1 241998 242999 ON 6 YFR045W (241998..242009,242082..242999) Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white YFR046C 0 243074 244159 ON 6 CNN1 complement(243074..244159) Kinetochore protein of unknown function; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by both Clb5-Cdk1 and, to a lesser extent, Clb2-Cdk1. YFR047C 0 244279 245166 ON 6 BNA6 complement(244279..245166) Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p YFR048W 1 246146 248134 ON 6 RMD8 (246146..248134) Cytosolic protein required for sporulation YFR049W 1 248523 248894 ON 6 YMR31 (248523..248894) Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 YFR050C 0 249066 249866 ON 6 PRE4 complement(249066..249866) Beta 7 subunit of the 20S proteasome YFR051C 0 250163 251803 ON 6 RET2 complement(250163..251803) Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER YFR052W 1 252505 253329 ON 6 RPN12 (252505..253329) Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p YFR052C-A 0 253429 253734 OFF 6 YFR052C-A complement(253429..253734) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFR053C 0 253592 255049 ON 6 HXK1 complement(253592..255049) Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p YFR054C 0 258855 259433 OFF 6 YFR054C complement(258855..259433) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFR056C 0 263957 264325 OFF 6 YFR056C complement(263957..264325) Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W YFR055W 1 264204 265226 ON 6 IRC7 (264204..265226) Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner YFR057W 1 269061 269516 OFF 6 YFR057W (269061..269516) Putative protein of unknown function YGL263W 1 2790 3932 ON 7 COS12 (2790..3932) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YGL262W 1 5312 5839 ON 7 YGL262W (5312..5839) Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene YGL261C 0 6290 6652 ON 7 PAU11 complement(6290..6652) Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 YGL260W 1 6860 7090 OFF 7 YGL260W (6860..7090) Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium YGL259W 1 8470 8967 ON 7 YPS5 (8470..8967) Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p YGL258W-A 1 9162 9395 ON 7 YGL258W-A (9162..9395) Putative protein of unknown function YGL258W 1 11110 11730 ON 7 VEL1 (11110..11730) Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants YGL257C 0 12481 14157 ON 7 MNT2 complement(12481..14157) Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans YGL256W 1 15159 16307 ON 7 ADH4 (15159..16307) Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency YGL255W 1 20978 22108 ON 7 ZRT1 (20978..22108) High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor YGL254W 1 22304 23203 ON 7 FZF1 (22304..23203) Transcription factor involved in sulfite metabolism, sole identified regulatory target is SSU1, overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers YGL253W 1 23935 25395 ON 7 HXK2 (23935..25395) Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene YGL252C 0 25718 27484 ON 7 RTG2 complement(25718..27484) Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p YGL251C 0 27921 31636 ON 7 HFM1 complement(27921..31426,31579..31636) Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity YGL250W 1 31910 32635 ON 7 RMR1 (31910..32635) Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus YGL249W 1 33098 35212 ON 7 ZIP2 (33098..35212) Meiosis-specific protein involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis YGL248W 1 35653 36762 ON 7 PDE1 (35653..36762) Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation YGL247W 1 36933 37526 ON 7 BRR6 (36933..37526) Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism YGL246C 0 37617 38780 ON 7 RAI1 complement(37617..38780) Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z YGL245W 1 39023 41149 ON 7 GUS1 (39023..41149) Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm YGL244W 1 41498 43174 ON 7 RTF1 (41498..43174) Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay YGL243W 1 43307 44509 ON 7 TAD1 (43307..44509) tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala YGL242C 0 44652 45197 ON 7 YGL242C complement(44652..45197) Putative protein of unknown function; deletion mutant is viable YGL241W 1 45445 48459 ON 7 KAP114 (45445..48459) Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation YGL240W 1 48613 49365 ON 7 DOC1 (48613..49365) Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain YGL239C 0 49431 49745 OFF 7 YGL239C complement(49431..49745) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene CSE1 YGL238W 1 49552 52434 ON 7 CSE1 (49552..52434) Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation YGL237C 0 52731 53528 ON 7 HAP2 complement(52731..53528) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding YGL236C 0 53787 55796 ON 7 MTO1 complement(53787..55796) Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants YGL235W 1 55279 55815 OFF 7 YGL235W (55279..55815) Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 YGL234W 1 56482 58890 ON 7 ADE5,7 (56482..58890) Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities YGL233W 1 59122 61854 ON 7 SEC15 (59122..61854) Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which mediates polarized targeting of vesicles to active sites of exocytosis; Sec15p associates with Sec4p and vesicles YGL232W 1 62075 63002 ON 7 TAN1 (62075..62131,62190..63002) Putative tRNA acetyltransferase, RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA YGL231C 0 63048 63620 ON 7 EMC4 complement(63048..63620) Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; human ortholog TMEM85 may function in apoptosis YGL230C 0 63772 64215 ON 7 YGL230C complement(63772..64215) Putative protein of unknown function; non-essential gene YGL229C 0 64503 66959 ON 7 SAP4 complement(64503..66959) Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p YGL228W 1 67598 69331 ON 7 SHE10 (67598..69331) Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest YGL227W 1 69671 72547 ON 7 VID30 (69671..72547) Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); binds FBPase; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm YGL226C-A 0 72749 73158 ON 7 OST5 complement(72749..72988,73138..73158) Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins YGL226W 1 73340 73711 ON 7 MTC3 (73340..73711) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Scer_7.trna1V 0 73829 73902 ON 7 tV-AAC complement(73829..73902) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YGL225W 1 76894 77907 ON 7 VRG4 (76894..77907) Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi YGL224C 0 78015 78857 ON 7 SDT1 complement(78015..78857) Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives YGL223C 0 79112 80365 ON 7 COG1 complement(79112..80365) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YGL222C 0 80659 81186 ON 7 EDC1 complement(80659..81186) RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress YGL221C 0 81426 82292 ON 7 NIF3 complement(81426..82292) Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL220W 1 82374 82736 ON 7 FRA2 (82374..82736) Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel YGL219C 0 82878 84257 ON 7 MDM34 complement(82878..84257) Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth YGL218W 1 83650 84300 OFF 7 YGL218W (83650..84300) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance YGL217C 0 84839 85180 OFF 7 YGL217C complement(84839..85180) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W YGL216W 1 84885 87302 ON 7 KIP3 (84885..87302) Kinesin-related motor protein involved in mitotic spindle positioning YGL215W 1 87981 89339 ON 7 CLG1 (87981..89339) Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 YGL214W 1 90010 90495 OFF 7 YGL214W (90010..90495) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C YGL213C 0 90054 91247 ON 7 SKI8 complement(90054..91247) Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype YGL212W 1 91432 92382 ON 7 VAM7 (91432..92382) Vacuolar SNARE protein that functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog YGL211W 1 92512 93591 ON 7 NCS6 (92512..93591) Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae YGL210W 1 93792 94460 ON 7 YPT32 (93792..94460) Rab family GTPase, very similar to Ypt31p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi YGL209W 1 95858 97006 ON 7 MIG2 (95858..97006) Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes including SUC2 (invertase); binds to Mig1p-binding sites in SUC2 promoter; under low glucose conditions Mig2p relocalizes to mitochondrion, where it regulates morphology, interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in promoting mitochondrial fusion or regulating morphology YGL208W 1 97338 98585 ON 7 SIP2 (97338..98585) One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane YGL207W 1 98969 102076 ON 7 SPT16 (98969..102076) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases YGL206C 0 102543 107504 ON 7 CHC1 complement(102543..107504) Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function YGL205W 1 108158 110404 ON 7 POX1 (108158..110404) Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Scer_7.trna2H 0 110625 110696 ON 7 tH-GUG complement(110625..110696) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YGL204C 0 111700 112005 OFF 7 YGL204C complement(111700..112005) Protein of unknown function; mRNA identified as translated by ribosome profiling data YGL203C 0 112475 114664 ON 7 KEX1 complement(112475..114664) Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Scer_7.trna3K 1 115488 115583 ON 7 tK-UUU (115488..115524,115548..115583) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YGL202W 1 116059 117561 ON 7 ARO8 (116059..117561) Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis YGL201C 0 117854 120907 ON 7 MCM6 complement(117854..120907) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Scer_7.trna4K 1 122269 122341 ON 7 tK-CUU (122269..122341) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YGL200C 0 122694 123305 ON 7 EMP24 complement(122694..123305) Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles YGL199C 0 123572 124042 OFF 7 YGL199C complement(123572..124042) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W YGL198W 1 123591 124298 ON 7 YIP4 (123591..124298) Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport YGL197W 1 124698 129161 ON 7 MDS3 (124698..129161) Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain YGL196W 1 129883 131169 ON 7 DSD1 (129883..131169) D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate YGL195W 1 131525 139543 ON 7 GCN1 (131525..139543) Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA YGL194C-A 0 139719 139961 ON 7 YGL194C-A complement(139719..139961) Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species YGL194C 0 140368 141726 ON 7 HOS2 complement(140368..141726) Histone deacetylase required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity YGL193C 0 141916 142227 OFF 7 YGL193C complement(141916..142227) Haploid-specific gene repressed by a1-alpha2, turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold YGL192W 1 142246 144048 ON 7 IME4 (142246..144048) Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids YGL191W 1 144808 145197 ON 7 COX13 (144808..145197) Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes YGL190C 0 145809 147389 ON 7 CDC55 complement(145809..147389) Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion YGL189C 0 148229 148588 ON 7 RPS26A complement(148229..148588) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similarity to rat S26 ribosomal protein YGL188C-A 0 148824 148964 OFF 7 YGL188C-A complement(148824..148964) Putative protein of unknown function YGL188C 0 149343 149516 OFF 7 YGL188C complement(149343..149516) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL187C 0 149704 150171 ON 7 COX4 complement(149704..150171) Subunit IV of cytochrome c oxidase, the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation YGL186C 0 151037 152776 ON 7 TPN1 complement(151037..152776) Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p YGL185C 0 153167 154306 ON 7 YGL185C complement(153167..154306) Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGL184C 0 154615 156012 ON 7 STR3 complement(154615..156012) Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p YGL183C 0 156543 157285 ON 7 MND1 complement(156543..157199,157283..157285) Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks YGL182C 0 157273 157596 OFF 7 YGL182C complement(157273..157596) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MND1/YGL183C YGL181W 1 157906 159096 ON 7 GTS1 (157906..159096) Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations YGL180W 1 160065 162758 ON 7 ATG1 (160065..162758) Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p YGL179C 0 163409 165091 ON 7 TOS3 complement(163409..165091) Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome YGL178W 1 167352 170571 ON 7 MPT5 (167352..167354,167995..170571) mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity YGL177W 1 167559 167906 OFF 7 YGL177W (167559..167906) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL176C 0 171415 173079 ON 7 YGL176C complement(171415..173079) Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype YGL175C 0 173285 174322 ON 7 SAE2 complement(173285..174322) Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents YGL174W 1 174545 175345 ON 7 BUD13 (174545..175345) Subunit of the RES complex, which is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern YGL173C 0 175527 180113 ON 7 XRN1 complement(175527..180113) Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved in mRNA decay; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance; activated by the scavenger decapping enzyme Dcs1p YGL172W 1 180700 182118 ON 7 NUP49 (180700..182118) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) YGL171W 1 182390 184084 ON 7 ROK1 (182390..184084) RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation YGL170C 0 184153 185394 ON 7 SPO74 complement(184153..185394) Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Scer_7.trna5K 0 185714 185786 ON 7 tK-CUU complement(185714..185786) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YGL169W 1 186059 187339 ON 7 SUA5 (186059..187339) Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family YGL168W 1 187464 187796 OFF 7 HUR1 (187464..187796) Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene YGL167C 0 187616 190468 ON 7 PMR1 complement(187616..190468) High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease YGL166W 1 191129 191806 ON 7 CUP2 (191129..191806) Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations YGL165C 0 191398 191976 OFF 7 YGL165C complement(191398..191976) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W YGL164C 0 191978 193300 ON 7 YRB30 complement(191978..193300) RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes YGL163C 0 193707 196403 ON 7 RAD54 complement(193707..196403) DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family YGL162W 1 198138 199037 ON 7 SUT1 (198138..199037) Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression YGL161C 0 199210 200142 ON 7 YIP5 complement(199210..200142) Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport YGL160W 1 200561 202273 ON 7 AIM14 (200561..202273) Protein with similarity to iron/copper reductases (FRE1-8), possibly involved in iron homeostasis; may interact with ribosomes; null mutant displays elevated frequency of mitochondrial genome loss YGL159W 1 202721 203833 ON 7 YGL159W (202721..203833) Putative protein of unknown function; deletion mutant has no detectable phenotype Scer_7.trna6L 1 205521 205634 ON 7 tL-CAA (205521..205558,205591..205634) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 53.40 YGL158W 1 207033 208571 ON 7 RCK1 (207033..208571) Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations YGL157W 1 209006 210049 ON 7 ARI1 (209006..210049) NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily YGL156W 1 210416 213667 ON 7 AMS1 (210416..213667) Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway YGL155W 1 214081 215211 ON 7 CDC43 (214081..215211) Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis YGL154C 0 215278 216096 ON 7 LYS5 complement(215278..216096) Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine YGL153W 1 216273 217298 ON 7 PEX14 (216273..217298) Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p YGL152C 0 216692 217369 OFF 7 YGL152C complement(216692..217369) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W YGL151W 1 217524 220922 ON 7 NUT1 (217524..220922) Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription YGL150C 0 221104 225573 ON 7 INO80 complement(221104..225573) ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription YGL149W 1 225572 225877 OFF 7 YGL149W (225572..225877) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. YGL148W 1 226399 227529 ON 7 ARO2 (226399..227529) Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids YGL147C 0 227754 228329 ON 7 RPL9A complement(227754..228329) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins YGL146C 0 228751 229686 ON 7 RRT6 complement(228751..229686) Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins YGL145W 1 230243 232348 ON 7 TIP20 (230243..232348) Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p YGL144C 0 232450 234507 ON 7 ROG1 complement(232450..234507) Protein with putative serine active lipase domain YGL143C 0 234717 235958 ON 7 MRF1 complement(234717..235958) Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability YGL142C 0 236269 238119 ON 7 GPI10 complement(236269..238119) Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog YGL141W 1 238353 241085 ON 7 HUL5 (238353..241085) Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD YGL140C 0 241353 245012 ON 7 YGL140C complement(241353..245012) Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains YGL139W 1 245716 248124 ON 7 FLC3 (245716..248124) Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER YGL138C 0 248494 249531 ON 7 YGL138C complement(248494..249531) Putative protein of unknown function; has no significant sequence similarity to any known protein YGL137W 1 249869 252738 ON 7 SEC27 (249869..249886,250087..252738) Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP YGL136C 0 252897 253859 ON 7 MRM2 complement(252897..253859) Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ YGL135W 1 254641 255294 ON 7 RPL1B (254641..255294) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Ap and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal YGL134W 1 255663 256964 ON 7 PCL10 (255663..256964) Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate YGL133W 1 257707 261501 ON 7 ITC1 (257707..261501) Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex YGL132W 1 261580 261915 OFF 7 YGL132W (261580..261915) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C YGL131C 0 261648 265859 ON 7 SNT2 complement(261648..265859) DNA binding protein with similarity to the S. pombe Snt2 protein; computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters YGL130W 1 266145 267524 ON 7 CEG1 (266145..267524) Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide YGL129C 0 267723 269075 ON 7 RSM23 complement(267723..269075) Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p YGL128C 0 269293 270144 ON 7 CWC23 complement(269293..270144) Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p YGL127C 0 270392 270775 ON 7 SOH1 complement(270392..270775) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits YGL126W 1 270997 272139 ON 7 SCS3 (270997..272139) Protein required for inositol prototrophy, identified as an ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; disputed role in the synthesis of inositol phospholipids from inositol YGL125W 1 272520 274322 ON 7 MET13 (272520..274322) Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway YGL124C 0 274780 276714 ON 7 MON1 complement(274780..276714) Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate YGL123W 1 277617 278381 ON 7 RPS2 (277617..278381) Protein component of the small (40S) subunit, essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p YGL123C-A 0 277634 277864 OFF 7 YGL123C-A complement(277634..277864) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene RPS2/YGL123W YGL122C 0 278946 280523 ON 7 NAB2 complement(278946..280523) Nuclear polyadenylated RNA-binding protein required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p YGL121C 0 280777 281157 ON 7 GPG1 complement(280777..281157) Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing YGL120C 0 281634 283937 ON 7 PRP43 complement(281634..283937) RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome YGL119W 1 284442 285947 ON 7 COQ8 (284442..285947) Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis Scer_7.trna7W 0 287350 287455 ON 7 tW-CCA complement(287350..287385,287420..287455) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YGL118C 0 288014 288451 OFF 7 YGL118C complement(288014..288451) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL117W 1 288512 289309 ON 7 YGL117W (288512..289309) Putative protein of unknown function YGL116W 1 289809 291641 ON 7 CDC20 (289809..291641) Cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C), which is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate YGL115W 1 292033 293001 ON 7 SNF4 (292033..293001) Activating gamma subunit of the AMP-activated Snf1p kinase complex (contains Snf1p and a Sip1p/Sip2p/Gal83p family member); activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis YGL114W 1 293460 295637 ON 7 YGL114W (293460..295637) Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters YGL113W 1 295932 297938 ON 7 SLD3 (295932..297938) Protein involved in the initiation of DNA replication, required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p YGL112C 0 298178 299728 ON 7 TAF6 complement(298178..299728) Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 YGL111W 1 299978 301369 ON 7 NSA1 (299978..301369) Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis YGL110C 0 301537 303411 ON 7 CUE3 complement(301537..303411) Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination YGL109W 1 303513 303836 OFF 7 YGL109W (303513..303836) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C YGL108C 0 303649 304071 ON 7 YGL108C complement(303649..304071) Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YGL107C 0 304333 306273 ON 7 RMD9 complement(304333..306273) Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes YGL106W 1 306560 307009 ON 7 MLC1 (306560..307009) Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition YGL105W 1 307437 308567 ON 7 ARC1 (307437..308567) Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids YGL104C 0 308713 310173 ON 7 VPS73 complement(308713..310173) Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family YGL103W 1 310967 311927 ON 7 RPL28 (310967..311015,311527..311927) Ribosomal protein of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a ribosomal proteins; may have peptidyl transferase activity; can mutate to cycloheximide resistance YGL102C 0 311505 311933 OFF 7 YGL102C complement(311505..311933) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein YGL101W 1 312193 312840 ON 7 YGL101W (312193..312840) Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p YGL100W 1 313234 314283 ON 7 SEH1 (313234..314283) Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 YGL099W 1 314631 316553 ON 7 LSG1 (314631..316553) Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Scer_snR82 1 316788 317055 ON 7 SNR82 (316788..317055) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U1110, U2349, and U2351 YGL098W 1 317342 318079 ON 7 USE1 (317342..318079) Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Scer_7.trna8H 1 319781 319852 ON 7 tH-GUG (319781..319852) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YGL097W 1 321782 323230 ON 7 SRM1 (321782..323230) Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p YGL096W 1 325331 326161 ON 7 TOS8 (325331..326161) Homeodomain-containing protein and putative transcription factor found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; similar to Cup9p transcription factor Scer_7.trna9E 1 328583 328654 ON 7 tE-UUC (328583..328654) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; can mutate to become a missense suppressor tRNA that inserts glutamate residues at lysine codons; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YGL095C 0 328874 330607 ON 7 VPS45 complement(328874..330607) Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment YGL094C 0 331118 334465 ON 7 PAN2 complement(331118..334465) Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes YGL093W 1 334886 337639 ON 7 SPC105 (334886..337639) Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components YGL092W 1 337906 341859 ON 7 NUP145 (337906..341859) Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 YGL091C 0 342056 343042 ON 7 NBP35 complement(342056..343042) Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases YGL090W 1 343319 344584 ON 7 LIF1 (343319..344584) Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein YGL089C 0 344791 345153 ON 7 MF(ALPHA)2 complement(344791..345153) Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 YGL088W 1 345836 346201 OFF 7 YGL088W (345836..346201) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Scer_snR10 1 345986 346230 ON 7 SNR10 (345986..346230) H/ACA box small nucleolar RNA (snoRNA); essential gene required for for preRNA processing, specifically for cleavage at sites A1 and A2; also guides pseudouridylation of large subunit (LSU) rRNA at position U2923 YGL087C 0 346406 346904 ON 7 MMS2 complement(346406..346808,346894..346904) Ubiquitin-conjugating enzyme variant involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p YGL086W 1 347119 349368 ON 7 MAD1 (347119..349368) Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p YGL085W 1 349616 350440 ON 7 LCL3 (349616..350440) Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS YGL084C 0 350616 352298 ON 7 GUP1 complement(350616..352298) Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport YGL083W 1 353058 355472 ON 7 SCY1 (353058..355472) Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase YGL082W 1 355827 356972 ON 7 YGL082W (355827..356972) Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene YGL081W 1 357377 358339 ON 7 YGL081W (357377..358339) Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis YGL080W 1 358636 359028 ON 7 FMP37 (358636..359028) Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia YGL079W 1 359444 360100 ON 7 KXD1 (359444..360100) Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome YGL078C 0 360288 361859 ON 7 DBP3 complement(360288..361859) RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype YGL077C 0 362225 363916 ON 7 HNM1 complement(362225..363916) Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol YGL076C 0 364335 365996 ON 7 RPL7A complement(364335..364964,365433..365526,365986..365996) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) Scer_snR39 0 365163 365251 OFF 7 SNR39 complement(365163..365251) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A807 Scer_snR39B 0 366374 366469 ON 7 SNR39B complement(366374..366469) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position G805 YGL075C 0 366925 368088 ON 7 MPS2 complement(366925..368088) Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p YGL074C 0 368596 368922 OFF 7 YGL074C complement(368596..368922) Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor YGL073W 1 368753 371254 ON 7 HSF1 (368753..371254) Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated YGL072C 0 371003 371362 OFF 7 YGL072C complement(371003..371362) Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 YGL071W 1 372012 374084 ON 7 AFT1 (372012..374084) Transcription factor involved in iron utilization and homeostasis; binds the consensus site PyPuCACCCPu and activates the expression of target genes in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm YGL070C 0 374459 374827 ON 7 RPB9 complement(374459..374827) RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription YGL069C 0 375050 375514 OFF 7 YGL069C complement(375050..375514) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance YGL068W 1 375087 375671 ON 7 MNP1 (375087..375671) Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth YGL067W 1 376101 377255 ON 7 NPY1 (376101..377255) NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes YGL066W 1 377609 379582 ON 7 SGF73 (377609..379582) SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay YGL065C 0 379760 381271 ON 7 ALG2 complement(379760..381271) Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol YGL064C 0 381523 383208 ON 7 MRH4 complement(381523..383208) Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function YGL063W 1 383654 384766 ON 7 PUS2 (383654..384766) Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification YGL063C-A 0 384502 384651 OFF 7 YGL063C-A complement(384502..384651) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YGL062W 1 385196 388732 ON 7 PYC1 (385196..388732) Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc2p but differentially regulated; mutations in the human homolog are associated with lactic acidosis YGL061C 0 388968 389711 ON 7 DUO1 complement(388968..389711) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YGL060W 1 390065 391990 ON 7 YBP2 (390065..391990) Central kinetochore associated protein that mediates mitotic progression; interacts with several central kinetochore proteins and the centromeric histone Cse4p; role in resistance to oxidative stress; similarity to Ybp1p and Slk19p YGL059W 1 392223 393698 ON 7 PKP2 (392223..393698) Mitochondrial protein kinase that negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p YGL058W 1 393986 394504 ON 7 RAD6 (393986..394504) Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p YGL057C 0 394966 395829 ON 7 GEP7 complement(394966..395829) Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL056C 0 396035 397618 ON 7 SDS23 complement(396035..397618) One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding YGL055W 1 398628 400160 ON 7 OLE1 (398628..400160) Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria YGL054C 0 400871 401287 ON 7 ERV14 complement(400871..401287) Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon Scer_7.trna10E 0 401527 401598 ON 7 tE-UUC complement(401527..401598) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YGL053W 1 402589 403302 ON 7 PRM8 (402589..403302) Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family YGL052W 1 403437 403742 OFF 7 YGL052W (403437..403742) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MST27 YGL051W 1 403687 404391 ON 7 MST27 (403687..404391) Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Scer_7.trna11R 1 405470 405541 ON 7 tR-UCU (405470..405541) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YGL050W 1 405776 406597 ON 7 TYW3 (405776..406597) tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions YGL049C 0 406860 409604 ON 7 TIF4632 complement(406860..409604) Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p YGL048C 0 410069 411286 ON 7 RPT6 complement(410069..411286) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle YGL047W 1 411552 412160 ON 7 ALG13 (411552..412160) Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases Scer_7.trna12V 0 412294 412367 ON 7 tV-AAC complement(412294..412367) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YGL045W 1 414102 415730 ON 7 RIM8 (414102..415730) Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family YGL044C 0 416146 417036 ON 7 RNA15 complement(416146..417036) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping YGL043W 1 417483 418412 ON 7 DST1 (417483..418412) General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites YGL042C 0 418288 418593 OFF 7 YGL042C complement(418288..418593) Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 YGL041C-B 0 418704 418886 OFF 7 YGL041C-B complement(418704..418886) Putative protein of unknown function; identified by fungal homology and RT-PCR YGL041W-A 1 418825 419289 ON 7 YGL041W-A (418825..419289) Putative protein of unknown function, conserved in fungi; identified by expression profiling and mass spectrometry YGL041C 0 419022 419225 OFF 7 YGL041C complement(419022..419225) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL040C 0 419527 420555 ON 7 HEM2 complement(419527..420555) Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Scer_7.trna13L 1 423092 423205 ON 7 tL-CAA (423092..423129,423162..423205) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YGL039W 1 423961 425007 ON 7 YGL039W (423961..425007) Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols YGL038C 0 425362 426804 ON 7 OCH1 complement(425362..426804) Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins YGL037C 0 427297 427947 ON 7 PNC1 complement(427297..427947) Nicotinamidase that converts nicotinamide to nicotinic acid as part of the NAD(+) salvage pathway, required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span YGL036W 1 428601 431330 ON 7 YGL036W (428601..431330) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene YGL035C 0 431548 433062 ON 7 MIG1 complement(431548..433062) Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase YGL034C 0 433214 433579 OFF 7 YGL034C complement(433214..433579) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL033W 1 435625 436351 ON 7 HOP2 (435625..435679,435750..436351) Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair YGL032C 0 436570 436833 ON 7 AGA2 complement(436570..436833) Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds YGL031C 0 437467 437934 ON 7 RPL24A complement(437467..437934) Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate YGL030W 1 439091 439638 ON 7 RPL30 (439091..439093,439324..439638) Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript YGL029W 1 440063 440425 ON 7 CGR1 (440063..440425) Protein involved in nucleolar integrity and processing of the pre-rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress Scer_7.trna14F 0 440716 440807 ON 7 tF-GAA complement(440716..440751,440771..440807) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YGL028C 0 441281 442909 ON 7 SCW11 complement(441281..442909) Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p YGL027C 0 443642 446143 ON 7 CWH41 complement(443642..446143) Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress YGL026C 0 446412 448535 ON 7 TRP5 complement(446412..448535) Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis YGL025C 0 448764 449957 ON 7 PGD1 complement(448764..449957) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor YGL024W 1 449788 450123 OFF 7 YGL024W (449788..450123) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C YGL023C 0 450197 452104 ON 7 PIB2 complement(450197..452104) Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 YGL022W 1 452404 454560 ON 7 STT3 (452404..454560) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis YGL021W 1 454785 457067 ON 7 ALK1 (454785..457067) Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk2p and to mammalian haspins YGL020C 0 457163 457870 ON 7 GET1 complement(457163..457870) Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance YGL019W 1 458156 458992 ON 7 CKB1 (458156..458992) Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases YGL018C 0 459110 459664 ON 7 JAC1 complement(459110..459664) Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix YGL017W 1 459854 461365 ON 7 ATE1 (459854..461365) Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway YGL016W 1 461666 464911 ON 7 KAP122 (461666..464911) Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance YGL015C 0 465043 465435 ON 7 YGL015C complement(465043..465435) Putative protein of unknown function; null mutants accumulate cargo in the Golgi YGL014W 1 466141 468807 ON 7 PUF4 (466141..468807) Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors YGL014C-A 0 466237 466398 OFF 7 YGL014C-A complement(466237..466398) Dubious open reading frame, unlikely to encode a functional protein; identified by expression profiling and mass spectrometry YGL013C 0 469092 472298 ON 7 PDR1 complement(469092..472298) Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes YGL012W 1 472855 474276 ON 7 ERG4 (472855..474276) C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol YGL011C 0 474489 475247 ON 7 SCL1 complement(474489..475247) Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria YGL010W 1 475545 476069 ON 7 YGL010W (475545..476069) Putative protein of unknown function; YGL010W is not an essential gene YGL009C 0 476313 478652 ON 7 LEU1 complement(476313..478652) Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway YGL008C 0 479910 482666 ON 7 PMA1 complement(479910..482666) Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] YGL007C-A 0 482941 483027 OFF 7 YGL007C-A complement(482941..483027) Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding YGL007W 1 483260 483637 OFF 7 BRP1 (483260..483637) Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 YGL006W-A 1 485423 485533 OFF 7 YGL006W-A (485423..485533) Putative protein of unknown function; identified by SAGE YGL006W 1 485921 489442 ON 7 PMC1 (485921..489442) Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a YGL005C 0 489708 490547 ON 7 COG7 complement(489708..490547) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YGL004C 0 490703 491956 ON 7 RPN14 complement(490703..491956) Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p YGL003C 0 492474 494174 ON 7 CDH1 complement(492474..494174) Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p YGL002W 1 494517 495167 ON 7 ERP6 (494517..495167) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL001C 0 495453 496502 ON 7 ERG26 complement(495453..496502) C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Scer_CEN7 0 496920 497038 ON 7 CEN7 complement(496920..497038) Chromosome VII centromere YGR001C 0 497133 498034 ON 7 YGR001C complement(497133..497365,497459..497937,498000..498034) Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae YGR002C 0 498476 499906 ON 7 SWC4 complement(498476..499906) Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex YGR003W 1 500132 502366 ON 7 CUL3 (500132..502366) Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 YGR004W 1 502938 504326 ON 7 PEX31 (502938..504326) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p YGR005C 0 504657 505859 ON 7 TFG2 complement(504657..505859) TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 YGR006W 1 506070 506825 ON 7 PRP18 (506070..506825) Splicing factor involved in the positioning of the 3' splice site during the second catalytic step of splicing, part of snRNP U5, interacts with Slu7p YGR007W 1 506969 507940 ON 7 ECT1 (506969..507940) Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases YGR008C 0 508110 508364 ON 7 STF2 complement(508110..508364) Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p YGR009C 0 509047 511002 ON 7 SEC9 complement(509047..511002) t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog YGR010W 1 511545 512732 ON 7 NMA2 (511545..512732) Nicotinic acid mononucleotide adenylyltransferase, involved in de novo and salvage synthesis of NAD(+) YGR011W 1 512497 512823 OFF 7 YGR011W (512497..512823) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR012W 1 513159 514340 ON 7 YGR012W (513159..514340) Putative cysteine synthase, localized to the mitochondrial outer membrane YGR013W 1 514554 516416 ON 7 SNU71 (514554..516416) Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart YGR014W 1 516943 520863 ON 7 MSB2 (516943..520863) Mucin family member involved in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate YGR015C 0 521090 522076 ON 7 YGR015C complement(521090..522076) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YGR016W 1 522261 522833 ON 7 YGR016W (522261..522833) Putative protein of unknown function YGR017W 1 523787 524680 ON 7 YGR017W (523787..524680) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm YGR018C 0 524365 524694 OFF 7 YGR018C complement(524365..524694) Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W YGR019W 1 525229 526644 ON 7 UGA1 (525229..526644) Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization YGR020C 0 526973 527329 ON 7 VMA7 complement(526973..527329) Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane YGR021W 1 527632 528504 ON 7 YGR021W (527632..528504) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR022C 0 529257 529586 OFF 7 YGR022C complement(529257..529586) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W YGR023W 1 529264 530919 ON 7 MTL1 (529264..530919) Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Scer_7.trna15D 1 531610 531681 ON 7 tD-GUC (531610..531681) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YGR024C 0 531883 532596 ON 7 THG1 complement(531883..532596) tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes YGR025W 1 532639 532941 OFF 7 YGR025W (532639..532941) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR026W 1 532985 533821 ON 7 YGR026W (532985..533821) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YGR027C 0 534132 534458 ON 7 RPS25A complement(534132..534458) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein Scer_7.trna16E 1 541850 541921 ON 7 tE-UUC (541850..541921) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YGR028W 1 542203 543291 ON 7 MSP1 (542203..543291) Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase YGR029W 1 543553 544205 ON 7 ERV1 (543553..543638,543722..544205) Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Scer_7.trna17D 0 544577 544648 ON 7 tD-GUC complement(544577..544648) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 Scer_snR46 1 545370 545566 ON 7 SNR46 (545370..545566) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2865 YGR030C 0 545681 546157 ON 7 POP6 complement(545681..546157) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YGR031W 1 546441 547469 ON 7 IMO32 (546441..547469) Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 YGR031C-A 0 546809 547300 OFF 7 NAG1 complement(546809..547300) Protein involved in yeast cell wall biogenesis; localizes to the cell periphery; production of Nag1p is dependent upon the presence of Slt2p and Rlm1p; gene is nested within and antisense to IMO32 YGR032W 1 548264 553951 ON 7 GSC2 (548264..553951) Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) YGR033C 0 554248 554967 ON 7 TIM21 complement(554248..554967) Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity YGR034W 1 555812 556672 ON 7 RPL26B (555812..555830,556308..556672) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Ap and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA YGR035C 0 557072 557422 ON 7 YGR035C complement(557072..557422) Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance YGR035W-A 1 557565 557786 OFF 7 YGR035W-A (557565..557786) Putative protein of unknown function YGR036C 0 558149 558868 ON 7 CAX4 complement(558149..558868) Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation YGR037C 0 559731 559994 ON 7 ACB1 complement(559731..559994) Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion YGR038W 1 560682 561350 ON 7 ORM1 (560682..561350) Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Scer_7.trna18S 0 561662 561743 ON 7 tS-AGA complement(561662..561743) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YGR039W 1 574888 575199 OFF 7 YGR039W (574888..575199) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 YGR040W 1 575398 576504 ON 7 KSS1 (575398..576504) Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains YGR041W 1 577487 579130 ON 7 BUD9 (577487..579130) Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole YGR042W 1 579476 580291 ON 7 YGR042W (579476..580291) Putative protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR043C 0 580435 581436 ON 7 NQM1 complement(580435..581436) Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift YGR044C 0 582990 583892 ON 7 RME1 complement(582990..583892) Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation YGR045C 0 583934 584296 OFF 7 YGR045C complement(583934..584296) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR046W 1 584895 586052 ON 7 TAM41 (584895..586052) Mitochondrial protein involved in protein import into the mitochondrial matrix; maintains the functional integrity of the TIM23 protein translocator complex; viability of null mutant is strain-dependent; mRNA is targeted to the bud YGR047C 0 586392 589469 ON 7 TFC4 complement(586392..589469) One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 YGR048W 1 589826 590911 ON 7 UFD1 (589826..590911) Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) YGR049W 1 591314 591877 ON 7 SCM4 (591314..591877) Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation YGR050C 0 592086 592442 OFF 7 YGR050C complement(592086..592442) Protein of unknown function; mRNA identified as translated by ribosome profiling data YGR051C 0 592906 593229 OFF 7 YGR051C complement(592906..593229) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene YGR052W 1 593596 594705 ON 7 FMP48 (593596..594705) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation YGR053C 0 594986 595837 ON 7 YGR053C complement(594986..595837) Putative protein of unknown function YGR054W 1 596693 598621 ON 7 YGR054W (596693..598621) Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A YGR055W 1 599417 601141 ON 7 MUP1 (599417..601141) High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake YGR056W 1 601661 604447 ON 7 RSC1 (601661..604447) Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook YGR057C 0 604773 605501 ON 7 LST7 complement(604773..605501) Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface YGR058W 1 606136 607143 ON 7 PEF1 (606136..607143) Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2 YGR059W 1 607563 609101 ON 7 SPR3 (607563..609101) Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Scer_snR48 1 609584 609696 ON 7 SNR48 (609584..609696) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at positions G2791 and G2793 YGR060W 1 610564 611493 ON 7 ERG25 (610564..611493) C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol YGR061C 0 611889 615965 ON 7 ADE6 complement(611889..615965) Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway YGR062C 0 616328 617278 ON 7 COX18 complement(616328..617278) Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC YGR063C 0 617516 617824 ON 7 SPT4 complement(617516..617824) Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions YGR064W 1 617621 617989 OFF 7 YGR064W (617621..617989) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C YGR065C 0 618077 619858 ON 7 VHT1 complement(618077..619858) High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin YGR066C 0 620921 621799 ON 7 YGR066C complement(620921..621799) Putative protein of unknown function YGR067C 0 622372 624786 ON 7 YGR067C complement(622372..624786) Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p YGR068C 0 625324 627084 ON 7 ART5 complement(625324..627084) Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane YGR068W-A 1 626294 626389 OFF 7 YGR068W-A (626294..626389) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with the uncharacterized ORF YGR068C YGR069W 1 627083 627418 OFF 7 YGR069W (627083..627418) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR070W 1 627806 631273 ON 7 ROM1 (627806..631273) GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP YGR071C 0 631481 634063 ON 7 ENV11 complement(631481..634063) Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YGR072W 1 634304 635467 ON 7 UPF3 (634304..635467) Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance YGR073C 0 635615 635986 OFF 7 YGR073C complement(635615..635986) Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein YGR074W 1 635712 636152 ON 7 SMD1 (635712..636152) Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1 YGR075C 0 636147 636875 ON 7 PRP38 complement(636147..636875) Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly YGR076C 0 637104 637577 ON 7 MRPL25 complement(637104..637577) Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan YGR077C 0 637744 639513 ON 7 PEX8 complement(637744..639513) Intraperoxisomal organizer of the peroxisomal import machinery, tightly associated with the lumenal face of the peroxisomal membrane, essential for peroxisome biogenesis, binds PTS1-signal receptor Pex5p YGR078C 0 639772 640371 ON 7 PAC10 complement(639772..640371) Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding YGR079W 1 640720 641832 ON 7 YGR079W (640720..641832) Putative protein of unknown function; YGR079W is not an essential gene YGR080W 1 642010 643008 ON 7 TWF1 (642010..643008) Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly YGR081C 0 643148 643780 ON 7 SLX9 complement(643148..643780) Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant YGR082W 1 644044 644595 ON 7 TOM20 (644044..644595) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins YGR083C 0 644860 646815 ON 7 GCD2 complement(644860..646815) Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YGR084C 0 647123 648142 ON 7 MRP13 complement(647123..648142) Mitochondrial ribosomal protein of the small subunit YGR085C 0 648383 648907 ON 7 RPL11B complement(648383..648907) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11 YGR086C 0 649598 650617 ON 7 PIL1 complement(649598..650617) Primary component of eisosomes, which are large immobile cell cortex structures associated with endocytosis; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family YGR087C 0 651290 652981 ON 7 PDC6 complement(651290..652981) Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation YGR088W 1 654634 656322 ON 7 CTT1 (654634..656322) Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide YGR089W 1 656960 659770 ON 7 NNF2 (656960..659770) Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Scer_7.trna19T 1 661749 661820 ON 7 tT-UGU (661749..661820) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: UGU | Cove Score: 77.84 YGR090W 1 662358 666071 ON 7 UTP22 (662358..666071) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YGR091W 1 666341 667825 ON 7 PRP31 (666341..667825) Splicing factor, component of the U4/U6-U5 snRNP complex YGR092W 1 668189 669907 ON 7 DBF2 (668189..669907) Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs YGR093W 1 670388 671911 ON 7 YGR093W (670388..671911) Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YGR094W 1 672186 675500 ON 7 VAS1 (672186..675500) Mitochondrial and cytoplasmic valyl-tRNA synthetase YGR095C 0 675671 676342 ON 7 RRP46 complement(675671..676342) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) YGR096W 1 676621 677565 ON 7 TPC1 (676621..677565) Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family YGR097W 1 678695 682135 ON 7 ASK10 (678695..682135) Component of RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; paralogous to Rgc1p YGR098C 0 682566 687458 ON 7 ESP1 complement(682566..687458) Separase, a caspase-like cysteine protease that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p YGR099W 1 687899 689965 ON 7 TEL2 (687899..689965) Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 YGR100W 1 690245 693097 ON 7 MDR1 (690245..693097) Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function YGR101W 1 693363 694403 ON 7 PCP1 (693363..694403) Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases YGR102C 0 694584 695135 ON 7 GTF1 complement(694584..695135) Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria YGR103W 1 695417 697234 ON 7 NOP7 (695417..697234) Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation YGR104C 0 697445 698368 ON 7 SRB5 complement(697445..698368) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance YGR105W 1 698599 698832 ON 7 VMA21 (698599..698832) Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) YGR106C 0 698989 699786 ON 7 VOA1 complement(698989..699786) Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Scer_7.trna20L 1 700675 700756 ON 7 tL-GAG (700675..700756) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: GAG | Cove Score: 64.51 Scer_7.trna21K 0 700953 701048 ON 7 tK-UUU complement(700953..700988,701012..701048) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.64 YGR107W 1 702667 703116 OFF 7 YGR107W (702667..703116) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR108W 1 703636 705051 ON 7 CLB1 (703636..705051) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome YGR109C 0 705359 706501 ON 7 CLB6 complement(705359..706501) B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Scer_7.trna22C 0 707108 707179 ON 7 tC-GCA complement(707108..707179) Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis; tRNA Type: Cys (C) | Anti-Codon: GCA | Cove Score: 75.04 YGR110W 1 713709 715046 ON 7 CLD1 (713709..715046) Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 YGR111W 1 715828 717030 ON 7 YGR111W (715828..717030) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR112W 1 717358 718527 ON 7 SHY1 (717358..718527) Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome YGR113W 1 718893 719924 ON 7 DAM1 (718893..719924) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments YGR114C 0 720265 720654 OFF 7 YGR114C complement(720265..720654) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 YGR115C 0 720372 721151 OFF 7 YGR115C complement(720372..721151) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C YGR116W 1 720409 724764 ON 7 SPT6 (720409..724764) Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region YGR117C 0 725049 726479 ON 7 YGR117C complement(725049..726479) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR118W 1 726974 727730 ON 7 RPS23A (726974..727038,727358..727730) Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Bp and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal YGR119C 0 728046 729671 ON 7 NUP57 complement(728046..729671) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) YGR120C 0 730033 730821 ON 7 COG2 complement(730033..730821) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Scer_7.trna23N 1 731137 731210 ON 7 tN-GUU (731137..731210) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YGR121C 0 731449 732927 ON 7 MEP1 complement(731449..732927) Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation YGR121W-A 1 733412 733627 OFF 7 YGR121W-A (733412..733627) Putative protein of unknown function YGR122W 1 733935 735143 ON 7 YGR122W (733935..735143) Probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic YGR122C-A 0 735630 735758 OFF 7 YGR122C-A complement(735630..735758) Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Scer_7.trna24R 1 736340 736411 ON 7 tR-UCU (736340..736411) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YGR123C 0 736662 738203 ON 7 PPT1 complement(736662..738203) Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Scer_7.trna25I 1 739122 739195 ON 7 tI-AAU (739122..739195) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YGR124W 1 739944 741662 ON 7 ASN2 (739944..741662) Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway YGR125W 1 742325 745435 ON 7 YGR125W (742325..745435) Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YGR126W 1 745835 746527 ON 7 YGR126W (745835..746527) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS YGR127W 1 746798 747736 ON 7 YGR127W (746798..747736) Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response YGR128C 0 747950 750091 ON 7 UTP8 complement(747950..750091) Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YGR129W 1 750400 751047 ON 7 SYF2 (750400..751047) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest YGR130C 0 751394 753844 ON 7 YGR130C complement(751394..753844) Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) YGR131W 1 754726 755250 ON 7 FHN1 (754726..755250) Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain YGR132C 0 755589 756452 ON 7 PHB1 complement(755589..756452) Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation YGR133W 1 756896 757447 ON 7 PEX4 (756896..757447) Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis YGR134W 1 757770 761138 ON 7 CAF130 (757770..761138) Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation YGR135W 1 761392 762168 ON 7 PRE9 (761392..762168) Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform YGR136W 1 762428 763153 ON 7 LSB1 (762428..763153) Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YGR137W 1 762888 763262 OFF 7 YGR137W (762888..763262) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR138C 0 763762 765606 ON 7 TPO2 complement(763762..765606) Polyamine transport protein specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; member of the major facilitator superfamily YGR139W 1 765726 766064 OFF 7 YGR139W (765726..766064) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR140W 1 767429 770299 ON 7 CBF2 (767429..770299) Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo YGR141W 1 770569 771972 ON 7 VPS62 (770569..771972) Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins YGR142W 1 772454 773686 ON 7 BTN2 (772454..773686) v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase Scer_7.trna26A 1 774349 774421 ON 7 tA-AGC (774349..774421) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YGR143W 1 775193 777508 ON 7 SKN1 (775193..777508) Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Scer_7.trna27G 1 779616 779687 ON 7 tG-UCC (779616..779687) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: UCC | Cove Score: 66.72 YGR144W 1 780399 781379 ON 7 THI4 (780399..781379) Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents YGR145W 1 781767 783890 ON 7 ENP2 (781767..783890) Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p YGR146C 0 784223 784858 ON 7 ECL1 complement(784223..784858) Protein of unknown function, affects chronological lifespan; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation YGR146C-A 0 785276 785437 OFF 7 YGR146C-A complement(785276..785437) Putative protein of unknown function YGR147C 0 786054 786920 ON 7 NAT2 complement(786054..786920) Protein with an apparent role in acetylation of N-terminal methionine residues YGR148C 0 787312 787779 ON 7 RPL24B complement(787312..787779) Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate YGR149W 1 789031 790329 ON 7 YGR149W (789031..790329) Putative protein of unknown function; predicted to be an integal membrane protein YGR150C 0 790459 793053 ON 7 CCM1 complement(790459..793053) Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Scer_7.trna28A 1 794417 794489 ON 7 tA-UGC (794417..794489) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: UGC | Cove Score: 76.57 YGR151C 0 794655 794990 OFF 7 YGR151C complement(794655..794990) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C YGR152C 0 794674 795492 ON 7 RSR1 complement(794674..795492) GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases YGR153W 1 796092 796745 ON 7 YGR153W (796092..796745) Putative protein of unknown function YGR154C 0 796798 797868 ON 7 GTO1 complement(796798..797868) Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization YGR155W 1 798543 800066 ON 7 CYS4 (798543..800066) Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria YGR156W 1 800546 801823 ON 7 PTI1 (800546..801823) Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 YGR157W 1 802440 805049 ON 7 CHO2 (802440..805049) Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis YGR158C 0 805269 806021 ON 7 MTR3 complement(805269..806021) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) YGR159C 0 806412 807656 ON 7 NSR1 complement(806412..807656) Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis YGR160W 1 807073 807684 OFF 7 YGR160W (807073..807684) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR161C 0 808629 809420 ON 7 RTS3 complement(808629..809420) Putative component of the protein phosphatase type 2A complex YGR161W-C 1 810227 810505 OFF 7 YGR161W-C (810227..810505) Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Scer_7.trna29V 1 823482 823555 ON 7 tV-AAC (823482..823555) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YGR162W 1 824059 826917 ON 7 TIF4631 (824059..826917) Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit YGR163W 1 827552 828577 ON 7 GTR2 (827552..828577) Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD YGR164W 1 828625 828960 OFF 7 YGR164W (828625..828960) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_7.trna30R 0 828723 828794 ON 7 tR-UCU complement(828723..828794) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YGR165W 1 829116 830153 ON 7 MRPS35 (829116..830153) Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets YGR166W 1 830515 832197 ON 7 TRS65 (830515..832197) Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response YGR167W 1 832456 833157 ON 7 CLC1 (832456..833157) Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component YGR168C 0 833352 834482 ON 7 YGR168C complement(833352..834482) Putative protein of unknown function; YGR168C is not an essential gene YGR169C 0 834689 835903 ON 7 PUS6 complement(834689..835903) tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability YGR169C-A 0 836387 836665 ON 7 YGR169C-A complement(836387..836665) Putative protein of unknown function YGR170W 1 837142 840558 ON 7 PSD2 (837142..840558) Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine YGR171C 0 840824 842551 ON 7 MSM1 complement(840824..842551) Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p YGR172C 0 842845 843591 ON 7 YIP1 complement(842845..843591) Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 YGR173W 1 843854 844960 ON 7 RBG2 (843854..844960) Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Scer_7.trna31G 1 845649 845719 ON 7 tG-GCC (845649..845719) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YGR174C 0 845893 846405 ON 7 CBP4 complement(845893..846405) Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex YGR174W-A 1 846655 846741 OFF 7 YGR174W-A (846655..846741) Putative protein of unknown function; predicted to have a role in cell budding based on computational "guilt by association" analysis YGR175C 0 846933 848423 ON 7 ERG1 complement(846933..848423) Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine YGR176W 1 848720 849067 OFF 7 YGR176W (848720..849067) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR177C 0 848829 850436 ON 7 ATF2 complement(848829..850436) Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking YGR178C 0 851047 853215 ON 7 PBP1 complement(851047..853215) Component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation YGR179C 0 853675 854895 ON 7 OKP1 complement(853675..854895) Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly YGR180C 0 855264 856301 ON 7 RNR4 complement(855264..856301) Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Scer_7.trna32L 0 857378 857491 ON 7 tL-CAA complement(857378..857421,857454..857491) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YGR181W 1 858287 858604 ON 7 TIM13 (858287..858604) Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane YGR182C 0 858551 858904 OFF 7 YGR182C complement(858551..858904) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W YGR183C 0 859063 859476 ON 7 QCR9 complement(859063..859260,859474..859476) Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex YGR184C 0 859901 865753 ON 7 UBR1 complement(859901..865753) E3 ubiquitin ligase (N-recognin), forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) YGR185C 0 866336 867520 ON 7 TYS1 complement(866336..867520) Cytoplasmic tyrosyl-tRNA synthetase, required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies YGR186W 1 867774 869981 ON 7 TFG1 (867774..869981) TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 YGR187C 0 870232 871416 ON 7 HGH1 complement(870232..871416) Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16) YGR188C 0 872044 875109 ON 7 BUB1 complement(872044..875109) Protein kinase involved in the cell cycle checkpoint into anaphase; forms complex with Mad1p and Bub3p crucial to preventing cell cycle progression into anaphase in the presence of spindle damage; CDC28-mediated phosphorylation at Bub1p-T566 is important for its degradation in anaphase and adaptation of the checkpoint to prolonged mitotic arrest, but not to its kinase activity or chromosome segregation function; associates with centromere DNA via Skp1p Scer_7.trna33K 1 876394 876466 ON 7 tK-CUU (876394..876466) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YGR189C 0 876669 878192 ON 7 CRH1 complement(876669..878192) Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Scer_7.trna34W 0 878710 878815 ON 7 tW-CCA complement(878710..878745,878780..878815) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YGR190C 0 880296 880661 OFF 7 YGR190C complement(880296..880661) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W YGR191W 1 880420 882231 ON 7 HIP1 (880420..882231) High-affinity histidine permease, also involved in the transport of manganese ions YGR192C 0 882812 883810 ON 7 TDH3 complement(882812..883810) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall YGR193C 0 884509 885741 ON 7 PDX1 complement(884509..885741) Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core YGR194C 0 886073 887875 ON 7 XKS1 complement(886073..887875) Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains YGR195W 1 888882 889622 ON 7 SKI6 (888882..889622) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) YGR196C 0 889733 892186 ON 7 FYV8 complement(889733..892186) Protein of unknown function, required for survival upon exposure to K1 killer toxin YGR197C 0 892497 894140 ON 7 SNG1 complement(892497..894140) Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance YGR198W 1 894693 897146 ON 7 YPP1 (894693..897146) Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene YGR199W 1 897502 899781 ON 7 PMT6 (897502..899781) Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases YGR200C 0 899904 902270 ON 7 ELP2 complement(899904..902270) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin YGR201C 0 902520 903197 ON 7 YGR201C complement(902520..903197) Putative protein of unknown function YGR202C 0 903474 904748 ON 7 PCT1 complement(903474..904748) Cholinephosphate cytidylyltransferase, also known as CTP:phosphocholine cytidylyltransferase, rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding YGR203W 1 905237 905683 ON 7 YCH1 (905237..905683) Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR204W 1 905934 908774 ON 7 ADE3 (905934..908774) Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine YGR204C-A 0 909061 909174 OFF 7 YGR204C-A complement(909061..909174) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YGR205W 1 909213 910085 ON 7 TDA10 (909213..910085) ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele YGR206W 1 910432 910737 ON 7 MVB12 (910432..910737) ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin YGR207C 0 910843 911628 ON 7 CIR1 complement(910843..911628) Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response YGR208W 1 911883 912812 ON 7 SER2 (911883..912812) Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source YGR209C 0 912913 913227 ON 7 TRX2 complement(912913..913227) Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance YGR210C 0 913503 914738 ON 7 YGR210C complement(913503..914738) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR211W 1 915241 916701 ON 7 ZPR1 (915241..916701) Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p) YGR212W 1 917040 918446 ON 7 SLI1 (917040..918446) N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin YGR213C 0 918512 919465 ON 7 RTA1 complement(918512..919465) Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein YGR214W 1 920575 921788 ON 7 RPS0A (920575..920664,921120..921788) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal YGR215W 1 922175 922507 ON 7 RSM27 (922175..922507) Mitochondrial ribosomal protein of the small subunit YGR216C 0 922633 924462 ON 7 GPI1 complement(922633..924462) Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs YGR217W 1 924696 930815 ON 7 CCH1 (924696..930815) Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Scer_7.trna35G 0 930953 931023 ON 7 tG-GCC complement(930953..931023) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YGR218W 1 932541 935795 ON 7 CRM1 (932541..935795) Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin YGR219W 1 936035 936376 OFF 7 YGR219W (936035..936376) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C YGR220C 0 936074 936883 ON 7 MRPL9 complement(936074..936883) Mitochondrial ribosomal protein of the large subunit YGR221C 0 937124 938992 ON 7 TOS2 complement(937124..938992) Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Scer_snR7-L 0 939459 939672 ON 7 SNR7-L complement(939459..939672) U5 spliceosomal RNA (U5 snRNA), long form; associated with spliceosome Scer_snR7-S 0 939494 939672 ON 7 SNR7-S complement(939494..939672) U5 spliceosomal RNA (U5 snRNA), short form; derived from SNR7-L by 3' end processing; associated with spliceosome YGR222W 1 939923 940804 ON 7 PET54 (939923..940804) Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing YGR223C 0 940869 942215 ON 7 HSV2 complement(940869..942215) Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization YGR224W 1 942806 944647 ON 7 AZR1 (942806..944647) Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole YGR225W 1 945145 947019 ON 7 AMA1 (945145..946327,946421..947019) Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis YGR226C 0 946792 947001 OFF 7 YGR226C complement(946792..947001) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W YGR227W 1 947420 948997 ON 7 DIE2 (947420..948997) Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 YGR228W 1 949365 949709 OFF 7 YGR228W (949365..949709) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C YGR229C 0 949382 950899 ON 7 SMI1 complement(949382..950899) Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity YGR230W 1 951894 952307 ON 7 BNS1 (951894..952307) Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis YGR231C 0 952548 953480 ON 7 PHB2 complement(952548..953480) Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation YGR232W 1 953960 954646 ON 7 NAS6 (953960..954646) Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 YGR233C 0 954674 958210 ON 7 PHO81 complement(954674..958210) Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p YGR234W 1 959904 961103 ON 7 YHB1 (959904..961103) Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses YGR235C 0 961360 962061 ON 7 MOS2 complement(961360..962061) Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture YGR236C 0 962530 962817 ON 7 SPG1 complement(962530..962817) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR237C 0 963298 965655 ON 7 YGR237C complement(963298..965655) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR238C 0 966039 968687 ON 7 KEL2 complement(966039..968687) Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate YGR239C 0 969187 970053 ON 7 PEX21 complement(969187..970053) Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex18p YGR240C 0 970771 973734 ON 7 PFK1 complement(970771..973734) Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes YGR240C-A 0 974577 974777 OFF 7 YGR240C-A complement(974577..974777) Putative protein of unknown function; identified by fungal homology and RT-PCR YGR241C 0 974875 976581 ON 7 YAP1802 complement(974875..976581) Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1801p, member of the AP180 protein family YGR242W 1 976415 976723 OFF 7 YGR242W (976415..976723) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C YGR243W 1 977336 977776 ON 7 FMP43 (977336..977776) Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses YGR244C 0 978036 979319 ON 7 LSC2 complement(978036..979319) Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate YGR245C 0 979765 982068 ON 7 SDA1 complement(979765..982068) Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis YGR246C 0 982482 984272 ON 7 BRF1 complement(982482..984272) TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB YGR247W 1 984966 985685 ON 7 CPD1 (984966..985685) Cyclic nucleotide phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression YGR248W 1 985972 986739 ON 7 SOL4 (985972..986739) 6-phosphogluconolactonase with similarity to Sol3p YGR249W 1 988049 989419 ON 7 MGA1 (988049..989419) Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants YGR250C 0 991176 993521 ON 7 YGR250C complement(991176..993521) Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid YGR251W 1 995639 996229 ON 7 NOP19 (995639..996229) Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes YGR252W 1 996869 998188 ON 7 GCN5 (996869..998188) Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay YGR253C 0 998358 999140 ON 7 PUP2 complement(998358..999140) Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta YGR254W 1 1000927 1002240 ON 7 ENO1 (1000927..1002240) Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose YGR255C 0 1002523 1003962 ON 7 COQ6 complement(1002523..1003962) Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Scer_7.trna36T 1 1004216 1004287 ON 7 tT-UGU (1004216..1004287) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: UGU | Cove Score: 77.84 YGR256W 1 1004624 1006102 ON 7 GND2 (1004624..1006102) 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone YGR257C 0 1006205 1007305 ON 7 MTM1 complement(1006205..1007305) Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor YGR258C 0 1007671 1010766 ON 7 RAD2 complement(1007671..1010766) Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein YGR259C 0 1012481 1012921 OFF 7 YGR259C complement(1012481..1012921) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W YGR260W 1 1012485 1014089 ON 7 TNA1 (1012485..1014089) High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) YGR261C 0 1014321 1016750 ON 7 APL6 complement(1014321..1016750) Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools YGR262C 0 1016974 1017759 ON 7 BUD32 complement(1016974..1017759) Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription YGR263C 0 1017967 1019241 ON 7 SAY1 complement(1017967..1019241) Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YGR264C 0 1019598 1021853 ON 7 MES1 complement(1019598..1021853) Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs YGR265W 1 1021648 1022058 OFF 7 YGR265W (1021648..1022058) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase YGR266W 1 1022656 1024761 ON 7 YGR266W (1022656..1024761) Protein of unknown function, predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane YGR267C 0 1025004 1025735 ON 7 FOL2 complement(1025004..1025735) GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway YGR268C 0 1026057 1026653 ON 7 HUA1 complement(1026057..1026653) Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly YGR269W 1 1026636 1026962 OFF 7 YGR269W (1026636..1026962) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C YGR270W 1 1027370 1031509 ON 7 YTA7 (1027370..1031509) Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p YGR270C-A 0 1027382 1027600 OFF 7 YGR270C-A complement(1027382..1027600) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YTA7/YGR270W YGR271W 1 1031791 1037694 ON 7 SLH1 (1031791..1037694) Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses YGR271C-A 0 1037800 1038501 ON 7 EFG1 complement(1037800..1038501) Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus YGR273C 0 1038715 1039239 ON 7 YGR273C complement(1038715..1039239) Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene YGR274C 0 1039895 1043095 ON 7 TAF1 complement(1039895..1043095) TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression YGR275W 1 1043276 1043749 ON 7 RTT102 (1043276..1043749) Component of both the SWI/SNF and RSC chromatin remodeling complexes, suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition YGR276C 0 1043819 1045480 ON 7 RNH70 complement(1043819..1045480) 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts YGR277C 0 1045644 1046561 ON 7 CAB4 complement(1045644..1046561) Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved YGR278W 1 1046731 1048464 ON 7 CWC22 (1046731..1048464) Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein YGR279C 0 1048798 1049958 ON 7 SCW4 complement(1048798..1049958) Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating YGR280C 0 1050910 1051725 ON 7 PXR1 complement(1050910..1051725) Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain YGR281W 1 1052824 1057257 ON 7 YOR1 (1052824..1057257) Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) YGR282C 0 1057783 1058724 ON 7 BGL2 complement(1057783..1058724) Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance YGR283C 0 1059015 1060040 ON 7 YGR283C complement(1059015..1060040) Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus YGR284C 0 1060658 1061590 ON 7 ERV29 complement(1060658..1061590) Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo YGR285C 0 1061852 1063153 ON 7 ZUO1 complement(1061852..1063153) Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p YGR286C 0 1063813 1064940 ON 7 BIO2 complement(1063813..1064940) Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant YGR287C 0 1067222 1068991 ON 7 IMA1 complement(1067222..1068991) Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization; has specificity for isomaltose, palatinose, and methyl-alpha-glucoside; member of the IMA isomaltase family YGR288W 1 1070293 1071714 ON 7 MAL13 (1070293..1071714) MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C YGR289C 0 1073963 1075813 ON 7 MAL11 complement(1073963..1075813) Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization YGR290W 1 1075482 1075925 OFF 7 YGR290W (1075482..1075925) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) YGR291C 0 1076283 1076504 OFF 7 YGR291C complement(1076283..1076504) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR292W 1 1076599 1078353 ON 7 MAL12 (1076599..1078353) Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose YGR293C 0 1079885 1080346 OFF 7 YGR293C complement(1079885..1080346) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR294W YGR294W 1 1080306 1080668 ON 7 PAU12 (1080306..1080668) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YGR295C 0 1081584 1082729 ON 7 COS6 complement(1081584..1082729) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YGR296W 1 1084864 1090591 ON 7 YRF1-3 (1084864..1084882,1085031..1090591) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YGR296C-A 0 1088870 1089445 OFF 7 YGR296C-A complement(1088870..1089445) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL07R YGR296C-B 0 1089746 1090228 OFF 7 YGR296C-B complement(1089746..1090228) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; contained completely within TEL07R YHL050C 0 445 3311 ON 8 YHL050C complement(445..1897,2671..3311) Putative protein of unknown function, potential Cdc28p substrate YHL050W-A 1 811 1293 OFF 8 YHL050W-A (811..1293) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHL049C 0 3726 4541 ON 8 YHL049C complement(3726..4541) Putative protein of unknown function YHL048C-A 0 5663 5797 OFF 8 YHL048C-A complement(5663..5797) Putative protein of unknown function; identified by expression profiling and mass spectrometry YHL048W 1 6401 7546 ON 8 COS8 (6401..7546) Nuclear membrane protein, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response YHL047C 0 8351 10213 ON 8 ARN2 complement(8351..10213) Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C YHL046W-A 1 9981 10307 OFF 8 YHL046W-A (9981..10307) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL046C 0 11923 12285 ON 8 PAU13 complement(11923..12285) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock YHL045W 1 12502 12849 OFF 8 YHL045W (12502..12849) Putative protein of unknown function; not an essential gene YHL044W 1 13565 14272 ON 8 YHL044W (13565..14272) Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern YHL043W 1 14901 15413 ON 8 ECM34 (14901..15413) Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YHL042W 1 15667 16119 ON 8 YHL042W (15667..16119) Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YHL041W 1 17392 17841 OFF 8 YHL041W (17392..17841) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YHL040C 0 19088 20971 ON 8 ARN1 complement(19088..20971) Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores YHL039W 1 21783 23540 ON 8 EFM1 (21783..23540) Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YHL038C 0 23617 25509 ON 8 CBP2 complement(23617..25509) Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene YHL037C 0 25778 26179 OFF 8 YHL037C complement(25778..26179) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL036W 1 26241 27881 ON 8 MUP3 (26241..27881) Low affinity methionine permease, similar to Mup1p YHL035C 0 27978 32756 ON 8 VMR1 complement(27978..32756) Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions YHL034W-A 1 33176 33637 OFF 8 YHL034W-A (33176..33637) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL034C 0 33193 34077 ON 8 SBP1 complement(33193..34077) Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 YHL033C 0 35255 36025 ON 8 RPL8A complement(35255..36025) Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits YHL032C 0 36379 38508 ON 8 GUT1 complement(36379..38508) Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p YHL031C 0 38815 39486 ON 8 GOS1 complement(38815..39486) v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 YHL030W-A 1 39074 39535 OFF 8 YHL030W-A (39074..39535) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene GOS1/YHL031C YHL030W 1 40084 45690 ON 8 ECM29 (40084..45690) Scaffold protein that assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats YHL029C 0 45929 47968 ON 8 OCA5 complement(45929..47968) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YHL028W 1 48763 50580 ON 8 WSC4 (48763..50580) ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 YHL027W 1 51111 52988 ON 8 RIM101 (51111..52988) Transcriptional repressor involved in response to pH and in cell wall construction; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by proteolytic processing; similar to A. nidulans PacC YHL026C 0 53219 54166 ON 8 YHL026C complement(53219..54166) Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) YHL025W 1 54851 55849 ON 8 SNF6 (54851..55849) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p YHL024W 1 56649 58790 ON 8 RIM4 (56649..58790) Putative RNA-binding protein required for the expression of early and middle sporulation genes YHL023C 0 59123 62563 ON 8 NPR3 complement(59123..62563) Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Scer_8.trna1H 1 62755 62826 ON 8 tH-GUG (62755..62826) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YHL022C 0 62961 64157 ON 8 SPO11 complement(62961..64157) Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation YHL021C 0 64462 65859 ON 8 AIM17 complement(64462..65859) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss YHL020C 0 66242 67456 ON 8 OPI1 complement(66242..67456) Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance YHL019W-A 1 67711 68304 OFF 8 YHL019W-A (67711..68304) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene APM2/YHL019C YHL019C 0 67731 69548 ON 8 APM2 complement(67731..69548) Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport YHL018W 1 69708 70070 ON 8 YHL018W (69708..70070) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS YHL017W 1 70276 71874 ON 8 YHL017W (70276..71874) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles YHL016C 0 72037 74244 ON 8 DUR3 complement(72037..74244) Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway YHL015W-A 1 74699 74782 OFF 8 YHL015W-A (74699..74782) Putative protein of unknown function YHL015W 1 75412 75777 ON 8 RPS20 (75412..75777) Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins YHL014C 0 76097 77314 ON 8 YLF2 complement(76097..77314) Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHL013C 0 77430 78353 ON 8 OTU2 complement(77430..78353) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization YHL012W 1 78936 80417 ON 8 YHL012W (78936..80417) Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase YHL011C 0 80654 81616 ON 8 PRS3 complement(80654..81616) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YHL010C 0 81964 83721 ON 8 ETP1 complement(81964..83721) Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences YHL009C 0 84068 85060 ON 8 YAP3 complement(84068..85060) Basic leucine zipper (bZIP) transcription factor Scer_8.trna2V 0 85298 85371 ON 8 tV-AAC complement(85298..85371) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YHL008C 0 92627 94510 ON 8 YHL008C complement(92627..94510) Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YHL007C 0 95118 97937 ON 8 STE20 complement(95118..97937) Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p YHL006W-A 1 97732 98085 OFF 8 YHL006W-A (97732..98085) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL006C 0 98343 98795 ON 8 SHU1 complement(98343..98795) Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function YHL005C 0 98828 99220 OFF 8 YHL005C complement(98828..99220) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W YHL004W 1 99219 100403 ON 8 MRP4 (99219..100403) Mitochondrial ribosomal protein of the small subunit YHL003C 0 100648 101883 ON 8 LAG1 complement(100648..101883) Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p YHL002C-A 0 102587 103075 OFF 8 YHL002C-A complement(102587..103075) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL002W 1 102612 103970 ON 8 HSE1 (102612..103970) Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes YHL001W 1 104277 105091 ON 8 RPL14B (104277..104405,104804..105091) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similarity to rat L14 ribosomal protein Scer_CEN8 0 105586 105703 ON 8 CEN8 complement(105586..105703) Chromosome VIII centromere YHR001W 1 106055 107368 ON 8 OSH7 (106055..107368) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YHR001W-A 1 107826 108122 ON 8 QCR10 (107826..107831,107895..108122) Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain YHR002W 1 108812 109885 ON 8 LEU5 (108812..109885) Mitochondrial carrier protein involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized YHR003C 0 110028 111317 ON 8 YHR003C complement(110028..111317) Protein of unknown function, localized to the mitochondrial outer membrane YHR004C 0 111754 113094 ON 8 NEM1 complement(111754..113094) Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard YHR005C 0 113499 114917 ON 8 GPA1 complement(113499..114917) GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome YHR005C-A 0 115620 115901 ON 8 TIM10 complement(115620..115901) Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Scer_8.trna3T 0 116107 116179 ON 8 tT-AGU complement(116107..116179) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YHR006W 1 117814 119439 ON 8 STP2 (117814..119439) Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes YHR007C 0 120091 121683 ON 8 ERG11 complement(120091..121683) Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p YHR007C-A 0 122550 122765 OFF 8 YHR007C-A complement(122550..122765) Putative protein of unknown function; identified by expression profiling and mass spectrometry YHR008C 0 122889 123590 ON 8 SOD2 complement(122889..123590) Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated YHR009C 0 124109 125680 ON 8 TDA3 complement(124109..125680) Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 YHR010W 1 126521 127492 ON 8 RPL27A (126521..126551,127113..127492) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similarity to rat L27 ribosomal protein YHR011W 1 127780 129120 ON 8 DIA4 (127780..129120) Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth YHR012W 1 129481 130448 ON 8 VPS29 (129481..129528,129648..130448) Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval YHR013C 0 130730 131446 ON 8 ARD1 complement(130730..131446) Subunit of N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells YHR014W 1 132047 132922 ON 8 SPO13 (132047..132922) Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Scer_8.trna4S 0 133026 133107 ON 8 tS-AGA complement(133026..133107) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 Scer_8.trna5Q 1 134321 134392 ON 8 tQ-UUG (134321..134392) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 YHR015W 1 134554 136533 ON 8 MIP6 (134554..136533) Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export YHR016C 0 136881 138455 ON 8 YSC84 complement(136881..138240,138409..138455) Actin-binding protein involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p YHR017W 1 138694 139851 ON 8 YSC83 (138694..139851) Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 YHR018C 0 140011 141402 ON 8 ARG4 complement(140011..141402) Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway YHR019C 0 141894 143558 ON 8 DED81 complement(141894..143558) Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA YHR020W 1 143996 146062 ON 8 YHR020W (143996..146062) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene Scer_8.trna6A 0 146242 146314 ON 8 tA-AGC complement(146242..146314) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YHR021C 0 147871 148669 ON 8 RPS27B complement(147871..148116,148667..148669) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Ap and has similarity to rat S27 ribosomal protein YHR021W-A 1 149225 149680 OFF 8 ECM12 (149225..149680) Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity YHR022C 0 149575 150345 ON 8 YHR022C complement(149575..150345) Putative protein of unknown function; YHR022C is not an essential gene YHR022C-A 0 151217 151306 OFF 8 YHR022C-A complement(151217..151306) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR023W 1 151666 157452 ON 8 MYO1 (151666..157452) Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively YHR024C 0 157744 159192 ON 8 MAS2 complement(157744..159192) Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins YHR025W 1 159438 160511 ON 8 THR1 (159438..160511) Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway YHR026W 1 160844 161485 ON 8 VMA16 (160844..161485) Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain YHR027C 0 161730 164711 ON 8 RPN1 complement(161730..164711) Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions YHR028C 0 164978 167434 ON 8 DAP2 complement(164978..167434) Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p YHR028W-A 1 167352 167672 OFF 8 YHR028W-A (167352..167672) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR029C 0 167677 168561 ON 8 YHI9 complement(167677..168561) Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production YHR030C 0 168890 170344 ON 8 SLT2 complement(168890..170344) Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway YHR031C 0 170799 172970 ON 8 RRM3 complement(170799..172970) DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p YHR032W 1 173344 175089 ON 8 ERC1 (173344..175089) Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine YHR032W-A 1 175195 175374 OFF 8 YHR032W-A (175195..175374) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032C-A YHR032C-A 0 175275 175394 OFF 8 YHR032C-A complement(175275..175394) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032W-A YHR033W 1 175548 176819 ON 8 YHR033W (175548..176819) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YHR034C 0 176965 177999 ON 8 PIH1 complement(176965..177999) Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II YHR035W 1 178219 180111 ON 8 YHR035W (178219..180111) Putative protein of unknown function; not an essential gene YHR036W 1 180345 181760 ON 8 BRL1 (180345..181760) Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export YHR037W 1 181977 183704 ON 8 PUT2 (181977..183704) Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism YHR038W 1 184066 184758 ON 8 RRF1 (184066..184758) Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria YHR039C 0 184875 186809 ON 8 MSC7 complement(184875..186809) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids YHR039C-A 0 187173 187679 ON 8 VMA10 complement(187173..187514,187677..187679) Subunit G of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification YHR040W 1 187924 189024 ON 8 BCD1 (187924..189024) Essential protein required for the accumulation of box C/D snoRNA YHR041C 0 189131 189864 ON 8 SRB2 complement(189131..189749,189851..189864) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance YHR042W 1 190543 192618 ON 8 NCP1 (190543..192618) NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p YHR043C 0 192805 193545 ON 8 DOG2 complement(192805..193545) 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed YHR044C 0 194068 194808 ON 8 DOG1 complement(194068..194808) 2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified YHR045W 1 195551 197233 ON 8 YHR045W (195551..197233) Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YHR046C 0 197398 198285 ON 8 INM1 complement(197398..198285) Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate YHR047C 0 198740 201310 ON 8 AAP1 complement(198740..201310) Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation YHR048W 1 204607 206151 ON 8 YHK8 (204607..206151) Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles YHR049W 1 206462 207193 ON 8 FSH1 (206462..207193) Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 YHR049C-A 0 207234 207530 OFF 8 YHR049C-A complement(207234..207530) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR050W 1 207654 209303 ON 8 SMF2 (207654..209303) Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins YHR050W-A 1 209475 209645 OFF 8 YHR050W-A (209475..209645) Protein of unknown function; identified by expression profiling and mass spectrometry YHR051W 1 209705 210151 ON 8 COX6 (209705..210151) Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels YHR052W 1 210848 211978 ON 8 CIC1 (210848..211978) Essential protein that interacts with proteasome components and has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles Scer_RUF5-1 1 212409 213118 OFF 8 RUF5-1 (212409..213118) RNA of Unknown Function YHR052W-A 1 212510 212704 OFF 8 YHR052W-A (212510..212704) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CUP1-1 YHR053C 0 212535 212720 ON 8 CUP1-1 complement(212535..212720) Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C YHR054C 0 213185 214249 OFF 8 YHR054C complement(213185..214249) Putative protein of unknown function Scer_RUF5-2 1 214407 215116 OFF 8 RUF5-2 (214407..215116) RNA of Unknown Function YHR054W-A 1 214508 214702 OFF 8 YHR054W-A (214508..214702) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CUP1-2 YHR055C 0 214533 214718 ON 8 CUP1-2 complement(214533..214718) Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C YHR056C 0 215183 217834 ON 8 RSC30 complement(215183..217834) Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc3p; null mutants are osmosensitive YHR056W-A 1 217406 217840 OFF 8 YHR056W-A (217406..217840) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR057C 0 218226 218843 ON 8 CPR2 complement(218226..218843) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway YHR058C 0 218997 219884 ON 8 MED6 complement(218997..219884) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YHR059W 1 220108 220500 ON 8 FYV4 (220108..220500) Protein of unknown function, required for survival upon exposure to K1 killer toxin YHR060W 1 220725 221270 ON 8 VMA22 (220725..221270) Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) YHR061C 0 221534 222478 ON 8 GIC1 complement(221534..222478) Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain YHR062C 0 222877 223758 ON 8 RPP1 complement(222877..223758) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YHR063C 0 224030 225169 ON 8 PAN5 complement(224030..225169) 2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE YHR063W-A 1 225442 225777 OFF 8 YHR063W-A (225442..225777) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR064C 0 225525 227141 ON 8 SSZ1 complement(225525..227141) Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP YHR065C 0 227532 229037 ON 8 RRP3 complement(227532..229037) Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity YHR066W 1 229335 230696 ON 8 SSF1 (229335..230696) Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family YHR067W 1 230970 231812 ON 8 HTD2 (230970..231812) Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology YHR068W 1 232133 233296 ON 8 DYS1 (232133..233296) Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric YHR069C 0 233579 234658 ON 8 RRP4 complement(233579..234658) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) YHR069C-A 0 234874 235236 OFF 8 YHR069C-A complement(234874..235236) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR070W 1 234881 236380 ON 8 TRM5 (234881..236380) tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya YHR070C-A 0 236102 236512 OFF 8 YHR070C-A complement(236102..236512) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene TRM5/YHR070W YHR071W 1 237004 237693 ON 8 PCL5 (237004..237693) Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Scer_8.trna7F 0 237848 237939 ON 8 tF-GAA complement(237848..237883,237903..237939) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YHR071C-A 0 238910 239230 OFF 8 YHR071C-A complement(238910..239230) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ERG7/YHR072W YHR072W 1 239098 241293 ON 8 ERG7 (239098..241293) Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis YHR072W-A 1 241664 241840 ON 8 NOP10 (241664..241840) Constituent of small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs, which are required for pseudouridylation and processing of pre-18S rRNA YHR073W 1 242582 245572 ON 8 OSH3 (242582..245572) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YHR073W-A 1 242868 243044 OFF 8 YHR073W-A (242868..243044) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry YHR073C-B 0 245422 245502 OFF 8 YHR073C-B complement(245422..245502) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry YHR074W 1 246193 248337 ON 8 QNS1 (246193..248337) Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide YHR075C 0 248439 249641 ON 8 PPE1 complement(248439..249641) Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein YHR076W 1 251101 252225 ON 8 PTC7 (251101..251155,251249..252225) Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope YHR077C 0 252374 255756 ON 8 NMD2 complement(252374..255637,255751..255756) Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance YHR078W 1 256360 258018 ON 8 YHR078W (256360..258018) High osmolarity-regulated gene of unknown function YHR079C 0 258244 261591 ON 8 IRE1 complement(258244..261591) Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress YHR079C-A 0 262192 262553 ON 8 SAE3 complement(262192..262354,262441..262553) Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p YHR080C 0 262801 266838 ON 8 YHR080C complement(262801..266838) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHR081W 1 267538 268092 ON 8 LRP1 (267538..268092) Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination YHR082C 0 268459 271548 ON 8 KSP1 complement(268459..271548) Ser/thr protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10 YHR083W 1 272627 273616 ON 8 SAM35 (272627..273616) Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane YHR084W 1 274174 276240 ON 8 STE12 (274174..276240) Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth YHR085W 1 276764 277768 ON 8 IPI1 (276764..277768) Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles YHR086W 1 278153 279724 ON 8 NAM8 (278153..279724) RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function YHR086W-A 1 280231 280392 OFF 8 YHR086W-A (280231..280392) Putative protein of unknown function; identified by fungal homology and RT-PCR YHR087W 1 280820 281155 ON 8 RTC3 (280820..281155) Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity YHR088W 1 281495 282382 ON 8 RPF1 (281495..282382) Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA YHR089C 0 282681 283298 ON 8 GAR1 complement(282681..283298) Protein component of the H/ACA snoRNP pseudouridylase complex, involved in the modification and cleavage of the 18S pre-rRNA YHR090C 0 283777 284625 ON 8 YNG2 complement(283777..284625) Subunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; has similarity to the human tumor suppressor ING1 and its isoforms ING4 and ING5 YHR091C 0 284839 286770 ON 8 MSR1 complement(284839..286770) Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 YHR092C 0 287081 288811 ON 8 HXT4 complement(287081..288811) High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose YHR093W 1 289142 289690 OFF 8 AHT1 (289142..289690) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region YHR094C 0 290913 292625 ON 8 HXT1 complement(290913..292625) Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting YHR095W 1 292943 293437 OFF 8 YHR095W (292943..293437) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR096C 0 294669 296447 ON 8 HXT5 complement(294669..296447) Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs YHR097C 0 297385 298609 ON 8 YHR097C complement(297385..298360,298485..298609) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus YHR098C 0 299145 301934 ON 8 SFB3 complement(299145..301934) Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p YHR099W 1 302761 313995 ON 8 TRA1 (302761..313995) Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation YHR100C 0 314116 314673 ON 8 GEP4 complement(314116..314673) Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT YHR101C 0 314874 315968 ON 8 BIG1 complement(314874..315771,315859..315968) Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan YHR102W 1 316572 319814 ON 8 KIC1 (316572..319814) Protein kinase of the PAK/Ste20 family, required for cell integrity; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; part of the RAM network that regulates cellular polarity and morphogenesis YHR103W 1 320414 322972 ON 8 SBE22 (320414..322972) Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth YHR104W 1 323409 324392 ON 8 GRE3 (323409..324392) Aldose reductase involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway YHR105W 1 324766 325410 ON 8 YPT35 (324766..325410) Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport YHR106W 1 325598 326626 ON 8 TRR2 (325598..326626) Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD YHR107C 0 326813 328036 ON 8 CDC12 complement(326813..328036) Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells YHR108W 1 328303 330060 ON 8 GGA2 (328303..330060) Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin YHR109W 1 330310 332067 ON 8 CTM1 (330310..332067) Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth YHR110W 1 332282 332920 ON 8 ERP5 (332282..332920) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport YHR111W 1 333072 334394 ON 8 UBA4 (333072..334394) Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p YHR112C 0 334527 335663 ON 8 YHR112C complement(334527..335663) Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway YHR113W 1 336337 337809 ON 8 APE4 (336337..337809) Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family YHR114W 1 338083 339984 ON 8 BZZ1 (338083..339984) SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins YHR115C 0 340109 341359 ON 8 DMA1 complement(340109..341359) Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains YHR116W 1 341665 342120 ON 8 COX23 (341665..342120) Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs YHR117W 1 342349 344268 ON 8 TOM71 (342349..344268) Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins YHR118C 0 344321 345628 ON 8 ORC6 complement(344321..345628) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p YHR119W 1 346043 349285 ON 8 SET1 (346043..349285) Histone methyltransferase, subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3K4; Set1p-dependent H3K4 trimethylation recruits Nrd1p, allowing efficient termination of snoRNAs and cryptic unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway; modulates histone acetylation levels in promoter proximal regions to ensure efficient Nrd1p-dependent termination; required in transcriptional silencing near telomeres and at silent mating type loci; has a SET domain YHR120W 1 349574 352453 ON 8 MSH1 (349574..352453) DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis YHR121W 1 352756 353319 ON 8 LSM12 (352756..353319) Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS YHR122W 1 353625 354320 ON 8 YHR122W (353625..354320) Protein of unknown function required for establishment of sister chromatid cohesion; synthetically lethal with RFC5, an RF-C subunit that links replication to cohesion establishment; YHR122W is an essential gene YHR123W 1 354815 356081 ON 8 EPT1 (354815..354864,354956..356081) sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability YHR124W 1 356561 358444 ON 8 NDT80 (356561..358444) Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Scer_8.trna8F 0 358478 358569 ON 8 tF-GAA complement(358478..358513,358533..358569) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YHR125W 1 358858 359163 OFF 8 YHR125W (358858..359163) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR126C 0 359702 360181 ON 8 ANS1 complement(359702..360181) Putative protein of unknown function; transcription dependent upon Azf1p YHR127W 1 360913 361644 ON 8 YHR127W (360913..361644) Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis YHR128W 1 362115 362765 ON 8 FUR1 (362115..362765) Uracil phosphoribosyltransferase, synthesizes UMP from uracil; involved in the pyrimidine salvage pathway YHR129C 0 362999 364153 ON 8 ARP1 complement(362999..364153) Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin YHR130C 0 364965 365300 OFF 8 YHR130C complement(364965..365300) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR131C 0 365340 367892 ON 8 YHR131C complement(365340..367892) Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study YHR131W-A 1 367747 367992 OFF 8 YHR131W-A (367747..367992) Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YHR131C YHR132C 0 368502 369794 ON 8 ECM14 complement(368502..369794) Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly YHR132W-A 1 370054 370449 ON 8 IGO2 (370054..370449) Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p YHR133C 0 370722 371597 ON 8 NSG1 complement(370722..371597) Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins YHR134W 1 371749 372558 ON 8 WSS1 (371749..372558) Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response YHR135C 0 372694 374310 ON 8 YCK1 complement(372694..374310) Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p YHR136C 0 374654 375100 ON 8 SPL2 complement(374654..375100) Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm YHR137W 1 375709 377250 ON 8 ARO9 (375709..377250) Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism YHR137C-A 0 375738 376388 OFF 8 YHR137C-A complement(375738..376388) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene ARO9/YHR137W; identified by gene-trapping, expression analysis, and genome-wide homology searching YHR138C 0 377355 377699 ON 8 YHR138C complement(377355..377699) Putative protein of unknown function; has similarity to Pbi2p; double null mutant lacking Pbi2p and Yhr138p exhibits highly fragmented vacuoles YHR139C 0 378219 379199 ON 8 SPS100 complement(378219..379199) Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin YHR139C-A 0 380108 380419 OFF 8 YHR139C-A complement(380108..380419) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR140W 1 380572 381291 ON 8 YHR140W (380572..381291) Putative integral membrane protein of unknown function Scer_snR32 1 381540 381727 ON 8 SNR32 (381540..381727) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2191 YHR141C 0 381990 382751 ON 8 RPL42B complement(381990..382306,382748..382751) Protein component of the large (60S) ribosomal subunit, identical to Rpl42Ap and has similarity to rat L44; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus YHR142W 1 383538 384488 ON 8 CHS7 (383538..384488) Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER YHR143W 1 385510 386487 ON 8 DSE2 (385510..386487) Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP YHR143W-A 1 387233 387445 ON 8 RPC10 (387233..387445) RNA polymerase subunit ABC10-alpha, found in RNA polymerase complexes I, II, and III YHR144C 0 387788 388726 ON 8 DCD1 complement(387788..388726) Deoxycytidine monophosphate (dCMP) deaminase; involved in dUMP and dTMP biosynthesis; expression is NOT cell cycle regulated Scer_8.trna9P 0 388893 388995 ON 8 tP-UGG complement(388893..388928,388960..388995) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 59.08 YHR145C 0 388981 389337 OFF 8 YHR145C complement(388981..389337) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR146W 1 390300 391697 ON 8 CRP1 (390300..391697) Protein that binds to cruciform DNA structures YHR147C 0 392639 393283 ON 8 MRPL6 complement(392639..393283) Mitochondrial ribosomal protein of the large subunit YHR148W 1 393534 394085 ON 8 IMP3 (393534..394085) Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA YHR149C 0 394455 396659 ON 8 SKG6 complement(394455..396659) Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p YHR150W 1 397251 398990 ON 8 PEX28 (397251..398990) Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p YHR151C 0 399268 400848 ON 8 MTC6 complement(399268..400848) Protein of unknown function; mtc6 is synthetically sick with cdc13-1 YHR152W 1 401434 401955 ON 8 SPO12 (401434..401955) Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis YHR153C 0 402086 402682 ON 8 SPO16 complement(402086..402682) Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation YHR154W 1 402966 406178 ON 8 RTT107 (402966..406178) Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition YHR155W 1 407103 410789 ON 8 YSP1 (407103..410789) Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone Scer_snR71 1 411228 411317 ON 8 SNR71 (411228..411317) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A2946 YHR156C 0 411384 412406 ON 8 LIN1 complement(411384..412406) Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication YHR157W 1 412907 413455 ON 8 REC104 (412907..413455) Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination YHR158C 0 413685 417179 ON 8 KEL1 complement(413685..417179) Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate YHR159W 1 417549 419063 ON 8 TDA11 (417549..419063) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele YHR160C 0 419221 420072 ON 8 PEX18 complement(419221..420072) Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p YHR161C 0 420373 422286 ON 8 YAP1801 complement(420373..422286) Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the AP180 protein family YHR162W 1 423072 423461 ON 8 MPC2 (423072..423461) Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium YHR163W 1 423724 424473 ON 8 SOL3 (423724..424473) 6-phosphogluconolactonase, catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p YHR164C 0 424610 429178 ON 8 DNA2 complement(424610..429178) Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization YHR165C 0 429707 436948 ON 8 PRP8 complement(429707..436948) Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutations of human Prp8 cause retinitis pigmentosa YHR165W-A 1 436647 436958 OFF 8 YHR165W-A (436647..436958) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR166C 0 437170 439050 ON 8 CDC23 complement(437170..439050) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YHR167W 1 439342 440127 ON 8 THP2 (439342..440127) Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance YHR168W 1 440377 441933 ON 8 MTG2 (440377..441933) Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly YHR169W 1 442181 443476 ON 8 DBP8 (442181..443476) ATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esf2p YHR170W 1 443828 445384 ON 8 NMD3 (443828..445384) Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex YHR171W 1 445713 447605 ON 8 ATG7 (445713..447605) Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation YHR172W 1 448335 450806 ON 8 SPC97 (448335..450806) Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque YHR173C 0 450815 451153 OFF 8 YHR173C complement(450815..451153) Protein of unknown function; expressed at both mRNA and protein levels YHR174W 1 451327 452640 ON 8 ENO2 (451327..452640) Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose YHR175W 1 452872 453441 ON 8 CTR2 (452872..453441) Putative low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation YHR175W-A 1 453558 453707 OFF 8 YHR175W-A (453558..453707) Putative protein of unknown function; identified by fungal homology and RT-PCR YHR176W 1 454229 455527 ON 8 FMO1 (454229..455527) Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins YHR177W 1 456591 457952 ON 8 YHR177W (456591..457952) Putative protein of unknown function; overexpression causes a cell cycle delay or arrest YHR178W 1 459299 461530 ON 8 STB5 (459299..461530) Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro YHR179W 1 462502 463704 ON 8 OYE2 (462502..463704) Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death YHR180W 1 465178 465669 OFF 8 YHR180W (465178..465669) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YHR180W-A 1 466932 467114 OFF 8 YHR180W-A (466932..467114) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YHR180C-B and long terminal repeat YHRCsigma3 YHR180C-B 0 466958 467062 OFF 8 YHR180C-B complement(466958..467062) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YHR180W-A Scer_8.trna10T 1 466990 467061 ON 8 tT-UGU (466990..467061) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: UGU | Cove Score: 79.33 YHR181W 1 467228 467914 ON 8 SVP26 (467228..467914) Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment YHR182W 1 468219 470576 ON 8 YHR182W (468219..470576) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm YHR182C-A 0 470939 471412 OFF 8 YHR182C-A complement(470939..471412) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR183W 1 470960 472429 ON 8 GND1 (470960..472429) 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress YHR184W 1 472744 474459 ON 8 SSP1 (472744..474459) Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis YHR185C 0 474627 475340 ON 8 PFS1 complement(474627..475340) Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Scer_8.trna11V 0 475706 475778 ON 8 tV-CAC complement(475706..475778) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: CAC | Cove Score: 75.97 YHR186C 0 475999 480672 ON 8 KOG1 complement(475999..480672) Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors YHR187W 1 480990 481919 ON 8 IKI1 (480990..481919) Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin YHR188C 0 482005 483837 ON 8 GPI16 complement(482005..483837) Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog YHR189W 1 484028 484600 ON 8 PTH1 (484028..484600) One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium YHR190W 1 484845 486179 ON 8 ERG9 (484845..486179) Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway YHR191C 0 486230 486631 ON 8 CTF8 complement(486230..486631) Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion YHR192W 1 486826 487662 ON 8 LNP1 (486826..487662) Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS YHR193C 0 487712 488236 ON 8 EGD2 complement(487712..488236) Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes YHR193C-A 0 488653 489027 OFF 8 YHR193C-A complement(488653..489027) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified ORF MDM31 YHR194W 1 488657 490396 ON 8 MDM31 (488657..490396) Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 YHR195W 1 490747 491712 ON 8 NVJ1 (490747..491712) Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) YHR196W 1 491931 493658 ON 8 UTP9 (491931..493658) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YHR197W 1 493896 496187 ON 8 RIX1 (493896..496187) Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles YHR198C 0 496315 497280 ON 8 AIM18 complement(496315..497280) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss YHR199C 0 497490 498422 ON 8 AIM46 complement(497490..498422) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss YHR199C-A 0 498571 498859 ON 8 NBL1 complement(498571..498719,498787..498859) Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates mitotic chromosome segregation; not required for the kinase activity of the complex; mediates the interaction of Sli15p and Bir1p YHR200W 1 499079 499885 ON 8 RPN10 (499079..499885) Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein YHR201C 0 499950 501143 ON 8 PPX1 complement(499950..501143) Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix YHR202W 1 502388 504196 ON 8 YHR202W (502388..504196) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization YHR203C 0 504476 505530 ON 8 RPS4B complement(504476..505247,505517..505530) Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein YHR204W 1 506319 508709 ON 8 MNL1 (506319..508709) Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation YHR205W 1 509363 511837 ON 8 SCH9 (509363..511837) AGC family protein kinase and functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan YHR206W 1 512732 514600 ON 8 SKN7 (512732..514600) Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation YHR207C 0 514905 516485 ON 8 SET5 complement(514905..516485) Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus YHR208W 1 517532 518713 ON 8 BAT1 (517532..518713) Mitochondrial branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA biosynthesis; homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase YHR209W 1 519437 520312 ON 8 CRG1 (519437..520312) S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin YHR210C 0 520712 521737 ON 8 YHR210C complement(520712..521737) Putative protein of unknown function; non-essential gene; highly expressed under anaeorbic conditions; sequence similarity to aldose 1-epimerases such as GAL10 YHR211W 1 525392 528619 ON 8 FLO5 (525392..528619) Lectin-like cell wall protein (flocculin) involved in flocculation, binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; similar to Flo1p YHR212C 0 537759 538094 OFF 8 YHR212C complement(537759..538094) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR212W-A 1 538742 538945 OFF 8 YHR212W-A (538742..538945) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR213W 1 539151 539747 ON 8 YHR213W (539151..539747) Possible pseudogene; has similarity to Flo1p, which is a lectin-like protein involved in flocculation YHR213W-A 1 540549 540782 OFF 8 YHR213W-A (540549..540782) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR213W-B 1 540800 541099 OFF 8 YHR213W-B (540800..541099) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR214W 1 541651 542262 ON 8 YHR214W (541651..542262) Putative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein YHR214W-A 1 543008 543493 OFF 8 YHR214W-A (543008..543493) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; similar to the fungal specific protein encoded by YAR068W; induced by zinc deficiency YHR214C-D 0 550648 550941 OFF 8 YHR214C-D complement(550648..550941) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR214C-E 0 551200 551499 OFF 8 YHR214C-E complement(551200..551499) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR215W 1 552099 553502 ON 8 PHO12 (552099..553502) One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; nearly identical to Pho11p; upregulated by phosphate starvation YHR216W 1 554396 555967 ON 8 IMD2 (554396..555967) Inosine monophosphate dehydrogenase, catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitatio YHR217C 0 556581 557042 OFF 8 YHR217C complement(556581..557042) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; located in the telomeric region TEL08R. YHR218W 1 558014 559924 ON 8 YHR218W (558014..558615,558715..559924) Helicase-like protein encoded within the telomeric Y' element YHR218W-A 1 559932 560249 OFF 8 YHR218W-A (559932..560249) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR219W 1 560173 562047 ON 8 YHR219W (560173..562047) Putative protein of unknown function with similarity to helicases; located in the telomere region on the right arm of chromosome VIII YHR219C-A 0 561199 561681 OFF 8 YHR219C-A complement(561199..561681) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YIL177C 0 483 6147 ON 9 YIL177C complement(483..4598,4987..6147) Putative protein of unknown function; similarity to DNA helicases that are encoded within subtelomeric Y' elements and induced in telomerase deficient survivors YIL177W-A 1 846 1328 OFF 9 YIL177W-A (846..1328) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YIL177C YIL176C 0 8793 9155 ON 9 PAU14 complement(8793..9155) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau1p YIL175W 1 9183 9500 OFF 9 YIL175W (9183..9500) Hypothetical protein YIL174W 1 9469 9696 OFF 9 YIL174W (9469..9696) Hypothetical protein YIL173W 1 11492 16141 ON 9 VTH1 (11492..16141) Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting YIL172C 0 16784 18553 ON 9 IMA3 complement(16784..18553) Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima3p overexpression allows the ima1 null mutant to grow on isomaltose YIL171W-A 1 18260 18712 OFF 9 YIL171W-A (18260..18712) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL171W 1 19515 19844 ON 9 YIL171W (19515..19844) Possible pseudogene in strain S288C; YIL171W and the adjacent ORF, YIL170W/HXT12, together encode a non-functional member of the hexose transporter family YIL170W 1 19847 21220 ON 9 HXT12 (19847..21220) Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family YIL169C 0 23119 26106 ON 9 YIL169C complement(23119..26106) Putative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; YIL169C is a non-essential gene Scer_YGOB_SDL1 1 29032 30048 ON 9 SDL1 (29032..29411,29415..30048) ANNOTATED BY YGOB - Skipping over stop codon in S288c YIL168W 1 29032 29415 OFF 9 YIL168W (29032..29415) Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase YIL167W 1 29416 30048 OFF 9 SDL1 (29416..30048) Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase YIL166C 0 30938 32566 ON 9 YIL166C complement(30938..32566) Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene YIL165C 0 33718 34077 ON 9 YIL165C complement(33718..34077) Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene YIL164C 0 34087 34686 ON 9 NIT1 complement(34087..34686) Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene YIL163C 0 36899 37252 OFF 9 YIL163C complement(36899..37252) Protein of unknown function; mRNA identified as translated by ribosome profiling data YIL162W 1 37385 38983 ON 9 SUC2 (37385..38983) Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively YIL161W 1 39433 40140 ON 9 YIL161W (39433..40140) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene YIL160C 0 40191 41444 ON 9 POT1 complement(40191..41444) 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids YIL159W 1 41825 45952 ON 9 BNR1 (41825..45952) Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 YIL158W 1 46201 46815 ON 9 AIM20 (46201..46815) Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss YIL157C 0 46949 47542 ON 9 COA1 complement(46949..47542) Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly YIL156W-A 1 47292 47693 OFF 9 YIL156W-A (47292..47693) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL156W-B 1 47690 47973 ON 9 YIL156W-B (47690..47698,47761..47973) Putative protein of unknown function, originally identified based on homology to Ashbya gossypii and other related yeasts YIL156W 1 48091 51306 ON 9 UBP7 (48091..51306) Ubiquitin-specific protease that cleaves ubiquitin-protein fusions YIL155C 0 51759 53708 ON 9 GUT2 complement(51759..53708) Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner YIL154C 0 53981 55021 ON 9 IMP2' complement(53981..55021) Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat YIL153W 1 55198 56379 ON 9 RRD1 (55198..56379) Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex YIL152W 1 56545 57252 ON 9 YIL152W (56545..57252) Putative protein of unknown function YIL151C 0 57338 60694 ON 9 YIL151C complement(57338..60694) Putative protein of unknown function, predicted to contain a PINc domain YIL150C 0 61013 62728 ON 9 MCM10 complement(61013..62728) Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins YIL149C 0 63028 68067 ON 9 MLP2 complement(63028..68067) Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length YIL148W 1 68708 69528 ON 9 RPL40A (68708..68715,69150..69528) Fusion protein, identical to Rpl40Bp, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes YIL147C 0 69791 73453 ON 9 SLN1 complement(69791..73453) Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators YIL146C 0 74184 75773 ON 9 ATG32 complement(74184..75773) Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane YIL145C 0 76354 77283 ON 9 PAN6 complement(76354..77283) Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC YIL144W 1 78074 80149 ON 9 TID3 (78074..80149) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering YIL143C 0 80510 83041 ON 9 SSL2 complement(80510..83041) Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 YIL142C-A 0 83208 83540 OFF 9 YIL142C-A complement(83208..83540) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL142W 1 83302 84885 ON 9 CCT2 (83302..84885) Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YIL141W 1 85053 85442 OFF 9 YIL141W (85053..85442) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL140W 1 85366 87837 ON 9 AXL2 (85366..87837) Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate YIL139C 0 87979 88716 ON 9 REV7 complement(87979..88716) Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair YIL138C 0 89230 89715 ON 9 TPM2 complement(89230..89715) Minor isoform of tropomyosin, binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p YIL137C 0 89948 92788 ON 9 TMA108 complement(89948..92788) Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease YIL136W 1 93619 94800 ON 9 OM45 (93619..94800) Protein of unknown function, major constituent of the mitochondrial outer membrane; located on the outer (cytosolic) face of the outer membrane YIL135C 0 95065 96375 ON 9 VHS2 complement(95065..96375) Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p YIL134C-A 0 96522 96725 OFF 9 YIL134C-A complement(96522..96725) Putative protein of unknown function; identified by fungal homology and RT-PCR Scer_snR68 1 97111 97246 ON 9 SNR68 (97111..97246) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A2640 YIL134W 1 97395 98330 ON 9 FLX1 (97395..98330) Protein required for transport of flavin adenine dinucleotide (FAD), a synthesis product of riboflavin, across the mitochondrial membrane YIL133C 0 98527 99416 ON 9 RPL16A complement(98527..99095,99386..99416) N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Bp, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p YIL132C 0 99860 100501 ON 9 CSM2 complement(99860..100501) Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis YIL131C 0 100781 102235 ON 9 FKH1 complement(100781..102235) Forkhead family transcription factor with a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; regulates donor preference during switching YIL130W 1 102782 105676 ON 9 ASG1 (102782..105676) Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance YIL129C 0 106107 113237 ON 9 TAO3 complement(106107..113237) Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p YIL128W 1 113806 116904 ON 9 MET18 (113806..116904) DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; involved in telomere maintenance YIL127C 0 117024 117644 ON 9 RRT14 complement(117024..117644) Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis YIL126W 1 117992 122071 ON 9 STH1 (117992..122071) ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p YIL125W 1 122689 125733 ON 9 KGD1 (122689..125733) Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA YIL124W 1 126204 127097 ON 9 AYR1 (126204..127097) NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones YIL123W 1 128151 129581 ON 9 SIM1 (128151..129581) Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated YIL122W 1 130610 131665 ON 9 POG1 (130610..131665) Nuclear chromatin-associated protein of unknown function; overexpression promotes recovery from pheromone induced arrest and suppresses the stress sensitivity caused by a mutation in the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; potential Cdc28p substrate; SBF regulated YIL121W 1 132244 133872 ON 9 QDR2 (132244..133872) Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin; may have a role in potassium uptake YIL120W 1 134417 136108 ON 9 QDR1 (134417..136108) Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, ketoconazole, fluconazole, and barban YIL119C 0 136654 137877 ON 9 RPI1 complement(136654..137877) Putative transcriptional regulator; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses the heat shock sensitivity of wild-type RAS2 overexpression and also suppresses the cell lysis defect of an mpk1 mutation YIL118W 1 139752 140447 ON 9 RHO3 (139752..140447) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p YIL117C 0 140613 141569 ON 9 PRM5 complement(140613..141569) Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling YIL116W 1 142928 144085 ON 9 HIS5 (142928..144085) Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts YIL115W-A 1 144268 144639 OFF 9 YIL115W-A (144268..144639) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene NUP159/YIL115C YIL115C 0 144327 148709 ON 9 NUP159 complement(144327..148709) FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) YIL114C 0 149143 149988 ON 9 POR2 complement(149143..149988) Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane permeability or mitochondrial osmotic stability YIL113W 1 150559 151188 ON 9 SDP1 (150559..151188) Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock YIL112W 1 151595 154846 ON 9 HOS4 (151595..154846) Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate YIL111W 1 155222 155765 ON 9 COX5B (155222..155222,155311..155765) Subunit Vb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth YIL110W 1 156045 157178 ON 9 HPM1 (156045..157178) AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance YIL109C 0 157385 160165 ON 9 SEC24 complement(157385..160165) Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p YIL108W 1 160887 162977 ON 9 YIL108W (160887..162977) Putative metalloprotease YIL107C 0 163278 165761 ON 9 PFK26 complement(163278..165761) 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A YIL106W 1 166415 167444 ON 9 MOB1 (166415..166434,166520..167444) Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex YIL105C 0 167581 169641 ON 9 SLM1 complement(167581..169641) Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex YIL105W-A 1 167664 167804 OFF 9 YIL105W-A (167664..167804) Protein of unknown function; completely overlaps the verified gene SLM1; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; mRNA identified as translated by ribosome profiling data YIL104C 0 169982 171505 ON 9 SHQ1 complement(169982..171505) Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones YIL103W 1 171751 173028 ON 9 DPH1 (171751..173028) Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p YIL102C-A 0 173365 173592 ON 9 YIL102C-A complement(173365..173592) Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species YIL102C 0 174582 174887 ON 9 YIL102C complement(174582..174887) Putative protein of unknown function Scer_9.trna1T 1 175031 175103 ON 9 tT-AGU (175031..175103) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YIL101C 0 175307 177250 ON 9 XBP1 complement(175307..177250) Transcriptional repressor that binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate YIL100C-A 0 177343 177681 OFF 9 YIL100C-A complement(177343..177681) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL100W 1 177373 177726 OFF 9 YIL100W (177373..177726) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A YIL099W 1 178004 179653 ON 9 SGA1 (178004..179653) Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation YIL098C 0 179772 180239 ON 9 FMC1 complement(179772..180239) Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p YIL097W 1 180427 181977 ON 9 FYV10 (180427..181977) Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; interacts with Rmd5p and contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo; contains CTLH domain; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin YIL096C 0 182117 183127 ON 9 YIL096C complement(182117..183127) Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Scer_9.trna2I 0 183440 183513 ON 9 tI-AAU complement(183440..183513) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YIL095W 1 183937 186369 ON 9 PRK1 (183937..186369) Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex YIL094C 0 186517 187632 ON 9 LYS12 complement(186517..187632) Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate YIL093C 0 187990 188784 ON 9 RSM25 complement(187990..188784) Mitochondrial ribosomal protein of the small subunit YIL092W 1 189066 190967 ON 9 YIL092W (189066..190967) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus YIL091C 0 191030 193195 ON 9 UTP25 complement(191030..193195) Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis YIL090W 1 193595 195070 ON 9 ICE2 (193595..195070) Integral ER membrane protein with type-III transmembrane domains; required for ER zinc homeostasis; mutations cause defects in cortical ER morphology in both the mother and daughter cells YIL089W 1 195599 196216 ON 9 YIL089W (195599..196216) Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Scer_9.trna3E 1 197592 197663 ON 9 tE-CUC (197592..197663) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; tRNA Type: Glu (E) | Anti-Codon: CUC | Cove Score: 59.79 YIL088C 0 197931 199403 ON 9 AVT7 complement(197931..199403) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YIL087C 0 199646 200119 ON 9 AIM19 complement(199646..200119) Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth YIL086C 0 200153 200461 OFF 9 YIL086C complement(200153..200461) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL085C 0 200490 202043 ON 9 KTR7 complement(200490..202043) Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YIL084C 0 202276 203259 ON 9 SDS3 complement(202276..203259) Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes YIL083C 0 203556 204653 ON 9 CAB2 complement(203556..204653) Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) YIL080W 1 205635 210357 OFF 9 YIL080W (205635..206484,206486..210357) TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition YIL082W 1 205635 206507 OFF 9 YIL082W (205635..206507) Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag Scer_9.trna4I 1 210665 210738 ON 9 tI-AAU (210665..210738) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YIL079C 0 210923 212005 ON 9 AIR1 complement(210923..212005) Zinc knuckle protein, involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; functionally redundant with Air2p YIL078W 1 212499 214703 ON 9 THS1 (212499..214703) Threonyl-tRNA synthetase, essential cytoplasmic protein YIL077C 0 214991 215953 ON 9 YIL077C complement(214991..215953) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL076W 1 216658 217548 ON 9 SEC28 (216658..217548) Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth YIL075C 0 217863 220700 ON 9 RPN2 complement(217863..220700) Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p YIL074C 0 221081 222490 ON 9 SER33 complement(221081..222490) 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser3p YIL073C 0 222937 225954 ON 9 SPO22 complement(222937..225809,225900..225954) Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis YIL072W 1 226602 228419 ON 9 HOP1 (226602..228419) Meiosis-specific DNA binding protein that displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for homologous chromosome synapsis and chiasma formation YIL071W-A 1 228550 229026 OFF 9 YIL071W-A (228550..229026) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL071C 0 228660 229994 ON 9 PCI8 complement(228660..229994) Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain YIL070C 0 230272 231072 ON 9 MAM33 complement(230272..231072) Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R YIL069C 0 231553 232369 ON 9 RPS24B complement(231553..231957,232367..232369) Protein component of the small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein YIL068W-A 1 233013 233396 OFF 9 YIL068W-A (233013..233396) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL068C 0 233057 235474 ON 9 SEC6 complement(233057..235474) Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesicles to active sites of exocytosis; dimeric form of Sec6p interacts with Sec9p in vitro and inhibits t-SNARE assembly YIL067C 0 235724 237760 ON 9 YIL067C complement(235724..237760) Uncharacterized protein of unknown function YIL066W-A 1 237366 237809 OFF 9 YIL066W-A (237366..237809) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL066C 0 238099 240708 ON 9 RNR3 complement(238099..240708) Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits YIL065C 0 241308 241775 ON 9 FIS1 complement(241308..241775) Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation YIL064W 1 241943 242716 ON 9 SEE1 (241943..242716) Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport YIL063C 0 242761 243744 ON 9 YRB2 complement(242761..243744) Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential YIL062C 0 243998 244462 ON 9 ARC15 complement(243998..244462) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity YIL061C 0 244657 245559 ON 9 SNP1 complement(244657..245559) Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein YIL060W 1 246392 246826 OFF 9 YIL060W (246392..246826) Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene YIL059C 0 246550 246915 OFF 9 YIL059C complement(246550..246915) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W YIL058W 1 246914 247198 OFF 9 YIL058W (246914..247198) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL057C 0 247902 248396 ON 9 RGI2 complement(247902..248396) Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Scer_9.trna5S 1 248850 248931 ON 9 tS-UGA (248850..248931) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Ser (S) | Anti-Codon: UGA | Cove Score: 84.16 YIL056W 1 249991 251913 ON 9 VHR1 (249991..251913) Transcriptional activator, required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations YIL055C 0 252042 253925 ON 9 YIL055C complement(252042..253925) Putative protein of unknown function YIL054W 1 254543 254860 OFF 9 YIL054W (254543..254860) Protein of unknown function; expressed at both mRNA and protein levels YIL053W 1 255115 255867 ON 9 RHR2 (255115..255867) Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress YIL052C 0 256226 257063 ON 9 RPL34B complement(256226..256554,257027..257063) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Ap and has similarity to rat L34 ribosomal protein YIL051C 0 257843 258280 ON 9 MMF1 complement(257843..258280) Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes YIL050W 1 258913 259770 ON 9 PCL7 (258913..259770) Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated YIL049W 1 260158 260919 ON 9 DFG10 (260158..260919) Probable polyprenol reductase that catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human ortholog SRD5A3 confer CDG (Congenital Disorders of Glycosylation) YIL048W 1 261437 264892 ON 9 NEO1 (261437..264892) Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus YIL047C-A 0 265099 265467 OFF 9 YIL047C-A complement(265099..265467) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL047C 0 265115 267823 ON 9 SYG1 complement(265115..267823) Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency YIL046W-A 1 268309 268473 OFF 9 YIL046W-A (268309..268473) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIL046W 1 268651 270573 ON 9 MET30 (268651..270573) F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus YIL045W 1 271161 272777 ON 9 PIG2 (271161..272777) Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase YIL044C 0 272950 273846 ON 9 AGE2 complement(272950..273846) ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif YIL043C 0 274072 274926 ON 9 CBR1 complement(274072..274926) Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia YIL042C 0 275108 276292 ON 9 PKP1 complement(275108..276292) Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p YIL041W 1 276525 277505 ON 9 GVP36 (276525..277505) BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis YIL040W 1 277723 278139 ON 9 APQ12 (277723..278139) Protein required for nuclear envelope morphology, nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism YIL039W 1 278426 279847 ON 9 TED1 (278426..279847) Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL038C 0 280142 282652 ON 9 NOT3 complement(280142..282652) Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation YIL037C 0 283029 284999 ON 9 PRM2 complement(283029..284999) Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion YIL036W 1 285666 287429 ON 9 CST6 (285666..287429) Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability YIL035C 0 287790 288908 ON 9 CKA1 complement(287790..288908) Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases YIL034C 0 289226 290089 ON 9 CAP2 complement(289226..290089) Beta subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches YIL033C 0 290419 291669 ON 9 BCY1 complement(290419..291669) Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation YIL032C 0 291961 292317 OFF 9 YIL032C complement(291961..292317) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL031W 1 292633 295737 ON 9 ULP2 (292633..295737) Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate YIL030W-A 1 295565 295903 OFF 9 YIL030W-A (295565..295903) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIL031W. YIL030C 0 296050 300009 ON 9 SSM4 complement(296050..300009) Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation YIL029W-A 1 299735 300106 OFF 9 YIL029W-A (299735..300106) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_9.trna6K 0 300228 300300 ON 9 tK-CUU complement(300228..300300) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 70.32 YIL029C 0 300829 301257 ON 9 YIL029C complement(300829..301257) Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL028W 1 302100 302498 OFF 9 YIL028W (302100..302498) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL027C 0 303679 304104 ON 9 EMC5 complement(303679..304104) Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance YIL026C 0 304477 307929 ON 9 IRR1 complement(304477..307929) Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability YIL025C 0 308201 308575 OFF 9 YIL025C complement(308201..308575) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL024C 0 308602 309171 ON 9 YIL024C complement(308602..309171) Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p YIL023C 0 309386 310426 ON 9 YKE4 complement(309386..310426) Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family YIL022W 1 311165 312460 ON 9 TIM44 (311165..312460) Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) YIL021W 1 312905 313861 ON 9 RPB3 (312905..313861) RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit YIL021C-A 0 313199 313468 OFF 9 YIL021C-A complement(313199..313468) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene RPB3/YIL021W; identified by gene-trapping, expression analysis, and genome-wide homology searching YIL020C-A 0 313713 314051 OFF 9 YIL020C-A complement(313713..314051) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL020C 0 314035 314820 ON 9 HIS6 complement(314035..314820) Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts YIL019W 1 315093 316133 ON 9 FAF1 (315093..316133) Protein required for pre-rRNA processing and 40S ribosomal subunit assembly YIL018W 1 316768 317932 ON 9 RPL2B (316768..316771,317172..317932) Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures YIL017C 0 318200 320965 ON 9 VID28 complement(318200..320965) Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm YIL016W 1 321454 321933 ON 9 SNL1 (321454..321933) Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein YIL015W 1 322342 324105 ON 9 BAR1 (322342..324105) Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Scer_9.trna7D 0 324303 324374 ON 9 tD-GUC complement(324303..324374) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YIL014C-A 0 325212 325526 ON 9 YIL014C-A complement(325212..325526) Putative protein of unknown function Scer_9.trna8T 1 325748 325820 ON 9 tT-AGU (325748..325820) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YIL014W 1 326103 327995 ON 9 MNT3 (326103..327995) Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation YIL013C 0 328207 332442 ON 9 PDR11 complement(328207..332442) ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromisedregulated by Pdr1p; required for anaerobic growth YIL012W 1 333011 333352 OFF 9 YIL012W (333011..333352) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL011W 1 333727 334536 ON 9 TIR3 (333727..334536) Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth YIL010W 1 334882 335529 ON 9 DOT5 (334882..335529) Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth YIL009C-A 0 335666 336212 ON 9 EST3 complement(335666..335935,335937..336212) Component of the telomerase holoenzyme, involved in telomere replication Scer_9.trna9D 0 336349 336420 ON 9 tD-GUC complement(336349..336420) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YIL009W 1 339344 341428 ON 9 FAA3 (339344..341428) Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YIL008W 1 342536 342835 ON 9 URM1 (342536..342835) Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response YIL007C 0 342994 343656 ON 9 NAS2 complement(342994..343656) Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p YIL006W 1 344062 345183 ON 9 YIA6 (344062..345183) Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs YIL005W 1 345692 347797 ON 9 EPS1 (345692..347797) ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family YIL004C 0 347946 348505 ON 9 BET1 complement(347946..348363,348495..348505) Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins YIL003W 1 349122 350003 ON 9 CFD1 (349122..350003) Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol YIL002W-A 1 350301 350510 ON 9 YIL002W-A (350301..350510) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIL002C 0 350591 353431 ON 9 INP51 complement(350591..353431) Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth YIL001W 1 353940 355481 ON 9 YIL001W (353940..355481) Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Scer_CEN9 1 355629 355745 ON 9 CEN9 (355629..355745) Chromosome IX centromere YIR001C 0 356143 356895 ON 9 SGN1 complement(356143..356895) Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation YIR002C 0 357415 360396 ON 9 MPH1 complement(357415..360396) 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases YIR003W 1 360885 362924 ON 9 AIM21 (360885..362924) Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton YIR004W 1 363221 364519 ON 9 DJP1 (363221..364519) Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ YIR005W 1 364889 365335 ON 9 IST3 (364889..365335) Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing YIR006C 0 365466 369908 ON 9 PAN1 complement(365466..369908) Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Scer_9.trna10E 1 370417 370488 ON 9 tE-UUC (370417..370488) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YIR007W 1 370704 372998 ON 9 YIR007W (370704..372998) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene YIR008C 0 373077 374306 ON 9 PRI1 complement(373077..374306) Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair YIR009W 1 374525 374860 ON 9 MSL1 (374525..374860) U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members YIR010W 1 375431 377161 ON 9 DSN1 (375431..377161) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation YIR011C 0 377287 378246 ON 9 STS1 complement(377287..378246) Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation YIR012W 1 378486 379781 ON 9 SQT1 (378486..379781) Essential protein involved in a late step of 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay YIR013C 0 380019 380384 ON 9 GAT4 complement(380019..380384) Protein containing GATA family zinc finger motifs YIR014W 1 381086 381814 ON 9 YIR014W (381086..381814) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene YIR015W 1 381948 382382 ON 9 RPR2 (381948..382382) Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits YIR016W 1 382628 383425 ON 9 YIR016W (382628..383425) Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; YIR016W is a non-essential gene YIR017C 0 383556 384119 ON 9 MET28 complement(383556..384119) Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism YIR017W-A 1 383566 384165 OFF 9 YIR017W-A (383566..384165) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR018W 1 384609 385346 ON 9 YAP5 (384609..385346) Basic leucine zipper (bZIP) iron-sensing transcription factor YIR018C-A 0 385564 385701 OFF 9 YIR018C-A complement(385564..385701) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIR019C 0 389572 393675 ON 9 MUC1 complement(389572..393675) GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Scer_ICR1 0 393884 397082 ON 9 ICR1 complement(393884..397082) Long intergenic ncRNA, has a key role in regulating transcription of the nearby protein-coding ORF MUC1; initiated far upstream from MUC1 and transcribed across much of the large promoter of MUC1, repressing MUC1 transcription in cis YIR020C 0 394255 394557 OFF 9 YIR020C complement(394255..394557) Protein of unknown function; mRNA identified as translated by ribosome profiling data YIR020W-A 1 394917 395159 OFF 9 YIR020W-A (394917..395159) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Scer_PWR1 1 395999 396939 ON 9 PWR1 (395999..396939) One of two long intergenic ncRNAs that play key roles in regulating transcription of the nearby protein-coding ORF MUC1; PWR1 promotes MUC1 transcription by interfering with ncRNA ICR1, which is transcribed across the MUC1 promoter YIR020C-B 0 397215 397949 OFF 9 YIR020C-B complement(397215..397949) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF MRS1 YIR021W 1 397294 398385 ON 9 MRS1 (397294..398385) Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA YIR021W-A 1 398514 398726 OFF 9 YIR021W-A (398514..398726) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIR022W 1 398733 399236 ON 9 SEC11 (398733..399236) 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum YIR023W 1 399777 402689 ON 9 DAL81 (399777..402689) Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism YIR023C-A 0 402383 402706 OFF 9 YIR023C-A complement(402383..402706) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR024C 0 402841 403491 ON 9 YIR024C complement(402841..403491) Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect YIR025W 1 403659 404765 ON 9 MND2 (403659..404765) Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase YIR026C 0 404873 405967 ON 9 YVH1 complement(404873..405967) Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases YIR027C 0 406260 407642 ON 9 DAL1 complement(406260..407642) Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression YIR028W 1 408468 410375 ON 9 DAL4 (408468..410375) Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR029W 1 410807 411838 ON 9 DAL2 (410807..411838) Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR030C 0 412036 412770 ON 9 DCG1 complement(412036..412770) Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain YIR030W-A 1 412899 413282 OFF 9 YIR030W-A (412899..413282) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR031C 0 413015 414679 ON 9 DAL7 complement(413015..414679) Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR032C 0 415030 415617 ON 9 DAL3 complement(415030..415617) Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression YIR033W 1 416124 419465 ON 9 MGA2 (416124..419465) ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting YIR034C 0 419615 420736 ON 9 LYS1 complement(419615..420736) Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity YIR035C 0 421026 421790 ON 9 YIR035C complement(421026..421790) Putative cytoplasmic protein of unknown function YIR036C 0 422074 422865 ON 9 IRC24 complement(422074..422865) Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci YIR036W-A 1 422635 423036 OFF 9 YIR036W-A (422635..423036) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR037W 1 423128 423619 ON 9 HYR1 (423128..423619) Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor YIR038C 0 423809 424513 ON 9 GTT1 complement(423809..424513) ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p YIR039C 0 430498 432111 ON 9 YPS6 complement(430498..432111) Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance YIR040C 0 433389 433721 OFF 9 YIR040C complement(433389..433721) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YIR041W 1 433929 434303 ON 9 PAU15 (433929..434303) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YIR042C 0 435273 435983 ON 9 YIR042C complement(435273..435983) Putative protein of unknown function; YIR042C is a non-essential gene YIR043C 0 437043 437735 OFF 9 YIR043C complement(437043..437735) Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family YIR044C 0 437994 438179 ON 9 YIR044C complement(437994..438179) Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family YJL225C 0 466 6130 ON 10 YJL225C complement(466..4581,4970..6130) Putative protein of unknown function; similarity to DNA helicases that are also encoded within subtelomeric Y' elements and induced in telomerase deficient survivors YJL225W-A 1 829 1311 OFF 10 YJL225W-A (829..1311) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YJL225C YJL223C 0 8776 9138 ON 10 PAU1 complement(8776..9138) Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme; identical to Pau14p YJL222W-B 1 9346 9483 OFF 10 YJL222W-B (9346..9483) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL222W-A 1 9452 9679 OFF 10 YJL222W-A (9452..9679) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL222W 1 11475 16124 ON 10 VTH2 (11475..16124) Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting YJL221C 0 16767 18536 ON 10 IMA4 complement(16767..18536) Alpha-glucosidase with broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima4p overexpression allows the ima1 null mutant to grow on isomaltose YJL220W 1 18243 18695 OFF 10 YJL220W (18243..18695) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL221C/FSP2 YJL219W 1 19497 21200 ON 10 HXT9 (19497..21200) Putative hexose transporter that is nearly identical to Hxt11p, has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p YJL218W 1 21973 22563 ON 10 YJL218W (21973..22563) Putative protein of unknown function, similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene YJL217W 1 23133 23729 ON 10 REE1 (23133..23729) Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle YJL216C 0 24341 26086 ON 10 IMA5 complement(24341..26086) Alpha-glucosidase with specificity for isomaltose, maltose, and palatinose; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose YJL215C 0 26412 26771 OFF 10 YJL215C complement(26412..26771) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL214W 1 26887 28596 ON 10 HXT8 (26887..28596) Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose YJL213W 1 32163 33158 ON 10 YJL213W (32163..33158) Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p YJL212C 0 33850 36249 ON 10 OPT1 complement(33850..36249) Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family YJL211C 0 36757 37200 OFF 10 YJL211C complement(36757..37200) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 YJL210W 1 36919 37734 ON 10 PEX2 (36919..37734) RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import YJL209W 1 38005 39969 ON 10 CBP1 (38005..39969) Mitochondrial protein that interacts with the 5'-untranslated region of the COB mRNA and has a role in its stability and translation; found in a complex at the inner membrane along with Pet309p YJL208C 0 40194 41183 ON 10 NUC1 complement(40194..41183) Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy YJL207C 0 41389 47433 ON 10 LAA1 complement(41389..47433) AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene YJL206C 0 47659 49935 ON 10 YJL206C complement(47659..49935) Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment YJL205C 0 50139 50443 ON 10 NCE101 complement(50139..50268,50412..50443) Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences YJL204C 0 50629 53151 ON 10 RCY1 complement(50629..53151) F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth YJL203W 1 53341 54183 ON 10 PRP21 (53341..54183) Subunit of the SF3a splicing factor complex, required for spliceosome assembly YJL202C 0 53943 54290 OFF 10 YJL202C complement(53943..54290) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex YJL201W 1 54379 56178 ON 10 ECM25 (54379..56178) Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p YJL200C 0 56444 58813 ON 10 ACO2 complement(56444..58813) Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Scer_10.trna1T 0 59100 59172 ON 10 tT-AGU complement(59100..59172) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YJL199C 0 59856 60182 OFF 10 MBB1 complement(59856..60182) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies YJL198W 1 60844 63489 ON 10 PHO90 (60844..63489) Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth YJL197W 1 63805 67569 ON 10 UBP12 (63805..67569) Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease present in the nucleus and cytoplasm that cleaves ubiquitin from ubiquitinated proteins YJL197C-A 0 65805 66086 OFF 10 YJL197C-A complement(65805..66086) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL197C/UBP12 YJL196C 0 67850 68782 ON 10 ELO1 complement(67850..68782) Elongase I, medium-chain acyl elongase, catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids YJL195C 0 69241 69942 OFF 10 YJL195C complement(69241..69942) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 YJL194W 1 69338 70879 ON 10 CDC6 (69338..70879) Essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p YJL193W 1 71366 72574 ON 10 YJL193W (71366..72574) Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect YJL192C 0 72710 73414 ON 10 SOP4 complement(72710..73414) ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER YJL191W 1 73787 74611 ON 10 RPS14B (73787..73796,74205..74611) Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins YJL190C 0 74910 75302 ON 10 RPS22A complement(74910..75302) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Bp and has similarity to E. coli S8 and rat S15a ribosomal proteins YJL189W 1 75933 76474 ON 10 RPL39 (75933..75938,76325..76474) Protein component of the large (60S) ribosomal subunit, has similarity to rat L39 ribosomal protein; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1 YJL188C 0 76202 76510 OFF 10 BUD19 complement(76202..76510) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay YJL187C 0 76803 79262 ON 10 SWE1 complement(76803..79262) Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate YJL186W 1 80155 81915 ON 10 MNN5 (80155..81915) Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment YJL185C 0 82095 82976 ON 10 ATG36 complement(82095..82976) Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene YJL184W 1 83448 83819 ON 10 GON7 (83448..83819) Component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Bud32p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; implicated in osmotic stress response YJL183W 1 84068 85336 ON 10 MNN11 (84068..85336) Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p YJL182C 0 85435 85752 OFF 10 YJL182C complement(85435..85752) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W YJL181W 1 85660 87495 ON 10 YJL181W (85660..87495) Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region YJL180C 0 87583 88560 ON 10 ATP12 complement(87583..88560) Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency YJL179W 1 88787 89116 ON 10 PFD1 (88787..89116) Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin YJL178C 0 89282 90097 ON 10 ATG27 complement(89282..90097) Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site YJL177W 1 90786 91657 ON 10 RPL17B (90786..91094,91412..91657) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similarity to E. coli L22 and rat L17 ribosomal proteins YJL176C 0 92053 94530 ON 10 SWI3 complement(92053..94530) Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2 YJL175W 1 94049 94561 OFF 10 YJL175W (94049..94561) Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor YJL174W 1 95092 95922 ON 10 KRE9 (95092..95922) Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects YJL173C 0 96161 96529 ON 10 RFA3 complement(96161..96529) Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination YJL172W 1 97737 99467 ON 10 CPS1 (97737..99467) Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions YJL171C 0 99704 100894 ON 10 YJL171C complement(99704..100894) GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein YJL170C 0 101150 101779 ON 10 ASG7 complement(101150..101779) Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor YJL169W 1 102099 102467 OFF 10 YJL169W (102099..102467) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 YJL168C 0 102227 104428 ON 10 SET2 complement(102227..104428) Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p YJL167W 1 105014 106072 ON 10 ERG20 (105014..106072) Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis YJL166W 1 106434 106718 ON 10 QCR8 (106434..106718) Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p YJL165C 0 106894 109461 ON 10 HAL5 complement(106894..109461) Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters YJL164C 0 109966 111159 ON 10 TPK1 complement(109966..111159) cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk2p and Tpk3p YJL163C 0 111666 113333 ON 10 YJL163C complement(111666..113333) Putative protein of unknown function YJL162C 0 113878 115629 ON 10 JJJ2 complement(113878..115629) Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Scer_10.trna2E 0 115939 116010 ON 10 tE-UUC complement(115939..116010) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YJL161W 1 117245 117787 ON 10 FMP33 (117245..117787) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL160C 0 117962 118825 ON 10 YJL160C complement(117962..118825) Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis YJL159W 1 120449 121690 ON 10 HSP150 (120449..121690) O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation YJL158C 0 122265 122948 ON 10 CIS3 complement(122265..122948) Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family YJL157C 0 123836 126328 ON 10 FAR1 complement(123836..126328) Cyclin-dependent kinase inhibitor that mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate YJL156W-A 1 126604 126825 OFF 10 YJL156W-A (126604..126825) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL156C 0 126853 128952 ON 10 SSY5 complement(126853..128952) Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p YJL155C 0 129285 130643 ON 10 FBP26 complement(129285..130643) Fructose-2,6-bisphosphatase, required for glucose metabolism YJL154C 0 131101 133935 ON 10 VPS35 complement(131101..133935) Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval YJL153C 0 134332 135933 ON 10 INO1 complement(134332..135933) Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element YJL152W 1 136174 136533 OFF 10 YJL152W (136174..136533) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL151C 0 136372 136773 ON 10 SNA3 complement(136372..136773) Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles YJL150W 1 137123 137425 OFF 10 YJL150W (137123..137425) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL149W 1 137379 139370 ON 10 DAS1 (137379..139370) Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Scer_snR128 0 139566 139691 ON 10 SNR128 complement(139566..139691) C/D box small nucleolar RNA (snoRNA), referred to as U14; essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA; guides 2'-O-methylation of small subunit (SSU) rRNA at position C414 Scer_snR190 0 139761 139950 ON 10 SNR190 complement(139761..139950) C/D box small nucleolar RNA (snoRNA) predicted to guide 2'-O-methylation of large subunit (LSU) rRNA at position G2395 but not shown to be active YJL148W 1 140437 141138 ON 10 RPA34 (140437..141138) RNA polymerase I subunit A34.5 YJL147C 0 141419 142567 ON 10 YJL147C complement(141419..142567) Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene YJL146W 1 143292 144701 ON 10 IDS2 (143292..144701) Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation YJL145W 1 145160 146044 ON 10 SFH5 (145160..146044) Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p YJL144W 1 146359 146673 ON 10 YJL144W (146359..146673) Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; GFP-fusion protein is induced by the DNA-damaging agent MMS YJL143W 1 147101 147577 ON 10 TIM17 (147101..147577) Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex YJL142C 0 147819 148211 OFF 10 IRC9 complement(147819..148211) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci YJL141C 0 147967 150390 ON 10 YAK1 complement(147967..150390) Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose YJL140W 1 150961 151626 ON 10 RPB4 (150961..151626) RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation YJL139C 0 151713 152999 ON 10 YUR1 complement(151713..152999) Mannosyltransferase of the KTR1 family, involved in protein N-glycosylation; located in the Golgi apparatus YJL138C 0 153504 154691 ON 10 TIF2 complement(153504..154691) Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G YJL137C 0 154985 156127 ON 10 GLG2 complement(154985..156127) Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin YJL136W-A 1 156169 156252 OFF 10 YJL136W-A (156169..156252) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by SAGE YJL136C 0 156550 157273 ON 10 RPS21B complement(156550..156789,157250..157273) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Ap and has similarity to rat S21 ribosomal protein YJL135W 1 157877 158194 OFF 10 YJL135W (157877..158194) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 YJL134W 1 158188 159417 ON 10 LCB3 (158188..159417) Long-chain base-1-phosphate phosphatase with specificity for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids YJL133C-A 0 159623 159847 ON 10 YJL133C-A complement(159623..159847) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL133W 1 160619 161563 ON 10 MRS3 (160619..161563) Iron transporter that mediates Fe2+ transport across the inner mitochondrial membrane; mitochondrial carrier family member, similar to and functionally redundant with Mrs4p; active under low-iron conditions; may transport other cations YJL132W 1 161914 164166 ON 10 YJL132W (161914..164166) Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene YJL131C 0 164278 165348 ON 10 AIM23 complement(164278..165348) Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss YJL130C 0 165723 172367 ON 10 URA2 complement(165723..172367) Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP YJL129C 0 173599 177306 ON 10 TRK1 complement(173599..177306) Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation YJL128C 0 178097 180103 ON 10 PBS2 complement(178097..180103) MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition YJL127W-A 1 180194 180310 OFF 10 YJL127W-A (180194..180310) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL127C-B 0 181551 181709 ON 10 YJL127C-B complement(181551..181709) Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii YJL127C 0 182298 184220 ON 10 SPT10 complement(182298..184220) Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box YJL126W 1 184501 185424 ON 10 NIT2 (184501..185424) Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member YJL125C 0 185528 186679 ON 10 GCD14 complement(185528..186679) Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression YJL124C 0 187126 187644 ON 10 LSM1 complement(187126..187644) Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs YJL123C 0 188004 189440 ON 10 MTC1 complement(188004..189440) Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 YJL122W 1 189716 190243 ON 10 ALB1 (189716..190243) Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p YJL121C 0 190374 191090 ON 10 RPE1 complement(190374..191090) D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress YJL120W 1 191022 191345 OFF 10 YJL120W (191022..191345) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO YJL119C 0 191572 191895 OFF 10 YJL119C complement(191572..191895) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL118W 1 191639 192298 ON 10 YJL118W (191639..192298) Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein YJL117W 1 192531 193466 ON 10 PHO86 (192531..193466) Endoplasmic reticulum (ER) resident protein required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles YJL116C 0 193860 194873 ON 10 NCA3 complement(193860..194873) Protein that functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p ) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin YJL115W 1 196287 197126 ON 10 ASF1 (196287..197126) Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Scer_10.trna3A 0 197313 197385 ON 10 tA-AGC complement(197313..197385) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 Scer_10.trna4D 1 204735 204806 ON 10 tD-GUC (204735..204806) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YJL112W 1 205305 207449 ON 10 MDV1 (205305..207449) Peripheral protein of the cytosolic face of the mitochondrial outer membrane, required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats YJL111W 1 207877 209529 ON 10 CCT7 (207877..209529) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance YJL110C 0 209922 211577 ON 10 GZF3 complement(209922..211577) GATA zinc finger protein and Dal80p homolog that negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p YJL109C 0 212000 217309 ON 10 UTP10 complement(212000..217309) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance YJL108C 0 217705 218856 ON 10 PRM10 complement(217705..218856) Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation YJL107C 0 218853 220016 OFF 10 YJL107C complement(218853..220016) Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi YJL106W 1 221390 223327 ON 10 IME2 (221390..223327) Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p YJL105W 1 225055 226737 ON 10 SET4 (225055..226737) Protein of unknown function, contains a SET domain YJL104W 1 227327 227776 ON 10 PAM16 (227327..227776) Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Scer_snR37 0 228094 228479 ON 10 SNR37 complement(228094..228479) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2944 YJL103C 0 229025 230881 ON 10 GSM1 complement(229025..230881) Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis YJL102W 1 231301 233760 ON 10 MEF2 (231301..233760) Mitochondrial elongation factor involved in translational elongation Scer_10.trna5R 1 233939 234011 ON 10 tR-ACG (233939..234011) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 70.24 YJL101C 0 234320 236356 ON 10 GSH1 complement(234320..236356) Gamma glutamylcysteine synthetase catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury YJL100W 1 237263 239086 ON 10 LSB6 (237263..239086) Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YJL099W 1 239414 241654 ON 10 CHS6 (239414..241654) Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane YJL098W 1 242082 245258 ON 10 SAP185 (242082..245258) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p YJL097W 1 245591 246244 ON 10 PHS1 (245591..246244) Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking YJL096W 1 246490 246975 ON 10 MRPL49 (246490..246975) Mitochondrial ribosomal protein of the large subunit YJL095W 1 247255 251691 ON 10 BCK1 (247255..251691) Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p YJL094C 0 251821 254442 ON 10 KHA1 complement(251821..254442) Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies YJL093C 0 254737 256812 ON 10 TOK1 complement(254737..256812) Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin YJL092W 1 257423 260947 ON 10 SRS2 (257423..260947) DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability YJL091C 0 261080 262552 ON 10 GWT1 complement(261080..262552) Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors YJL090C 0 262757 265051 ON 10 DPB11 complement(262757..265051) Replication initiation protein that loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1 YJL089W 1 265926 268415 ON 10 SIP4 (265926..268415) C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus YJL088W 1 268799 269815 ON 10 ARG3 (268799..269815) Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine YJL087C 0 270002 272485 ON 10 TRL1 complement(270002..272485) tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes YJL086C 0 272478 272846 OFF 10 YJL086C complement(272478..272846) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 YJL085W 1 272827 274698 ON 10 EXO70 (272827..274698) Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which directs secretory vesicles to active sites of exocytosis; acts with Sec3p in membrane targeting of the exocyst via PI(4,5)P2 binding YJL084C 0 274862 278002 ON 10 ALY2 complement(274862..278002) Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins YJL083W 1 278841 280655 ON 10 TAX4 (278841..280655) EH domain-containing protein involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS YJL082W 1 281185 283380 ON 10 IML2 (281185..283380) Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL081C 0 283802 285271 ON 10 ARP4 complement(283802..285271) Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes YJL080C 0 285558 289226 ON 10 SCP160 complement(285558..289226) Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins YJL079C 0 289875 290774 ON 10 PRY1 complement(289875..290774) Protein of unknown function YJL078C 0 291336 293981 ON 10 PRY3 complement(291336..293981) Cell wall protein with a role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p YJL077W-B 1 294047 294145 OFF 10 YJL077W-B (294047..294145) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YJL077C 0 294666 295061 OFF 10 ICS3 complement(294666..295061) Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 YJL077W-A 1 294799 294885 OFF 10 YJL077W-A (294799..294885) Protein of unknown function; mRNA identified as translated by ribosome profiling data; completely overlaps the verified gene YJL077C/ICS3 YJL076W 1 295245 298814 ON 10 NET1 (295245..298814) Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure YJL075C 0 298460 298876 OFF 10 APQ13 complement(298460..298876) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate YJL074C 0 299157 302849 ON 10 SMC3 complement(299157..302849) Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member YJL073W 1 303181 305118 ON 10 JEM1 (303181..305118) DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 YJL072C 0 305221 305862 ON 10 PSF2 complement(305221..305862) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YJL071W 1 306132 307856 ON 10 ARG2 (306132..307856) Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p YJL070C 0 307971 310637 ON 10 YJL070C complement(307971..310637) Protein of unknown function; some similarity to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; not an essential gene YJL069C 0 310922 312706 ON 10 UTP18 complement(310922..312706) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YJL068C 0 313016 313915 ON 10 YJL068C complement(313016..313915) Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D YJL067W 1 314084 314434 OFF 10 YJL067W (314084..314434) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL066C 0 314114 314872 ON 10 MPM1 complement(314114..314872) Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches YJL065C 0 315054 315557 ON 10 DLS1 complement(315054..315557) Subunit of ISW2/yCHRAC chromatin accessibility complex along with Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing YJL064W 1 315175 315570 OFF 10 YJL064W (315175..315570) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 YJL063C 0 315759 316475 ON 10 MRPL8 complement(315759..316475) Mitochondrial ribosomal protein of the large subunit YJL062W-A 1 316723 316980 ON 10 COA3 (316723..316980) Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly YJL062W 1 317284 319776 ON 10 LAS21 (317284..319776) Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity YJL061W 1 320016 322157 ON 10 NUP82 (320016..322157) Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) YJL060W 1 323386 324720 ON 10 BNA3 (323386..324720) Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate YJL059W 1 324964 326190 ON 10 YHC3 (324964..326190) Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) YJL058C 0 326242 327873 ON 10 BIT61 complement(326242..327873) Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity YJL057C 0 328118 330121 ON 10 IKS1 complement(328118..330121) Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p YJL056C 0 330431 333073 ON 10 ZAP1 complement(330431..333073) Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains YJL055W 1 333357 334094 ON 10 YJL055W (333357..334094) Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU YJL054W 1 334265 335701 ON 10 TIM54 (334265..335701) Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane YJL053W 1 335898 337037 ON 10 PEP8 (335898..337037) Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport YJL052C-A 0 337884 338003 OFF 10 YJL052C-A complement(337884..338003) Putative protein of unknown function, identified based on comparison to related yeast species YJL052W 1 338271 339269 ON 10 TDH1 (338271..339269) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall YJL051W 1 339787 342255 ON 10 IRC8 (339787..342255) Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc YJL050W 1 342522 345743 ON 10 MTR4 (342522..345743) ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of the TRAMP complex and in TRAMP-independent processes; contains an arch domain, composed of two coiled-coil arms/stalks and a globular fist/KOW domain, that has RNA binding activity and is required for 5.8S rRNA processing YJL049W 1 345973 347325 ON 10 YJL049W (345973..347325) Putative protein of unknown function; YJL049W is a non-essential gene YJL048C 0 347447 348637 ON 10 UBX6 complement(347447..348637) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline YJL047C-A 0 349044 349178 OFF 10 YJL047C-A complement(349044..349178) Putative protein of unknown function Scer_snR60 0 349130 349233 ON 10 SNR60 complement(349130..349233) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at positions A817 and G908 YJL047C 0 349580 352108 ON 10 RTT101 complement(349580..352108) Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p YJL046W 1 352386 353615 ON 10 AIM22 (352386..353615) Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Scer_10.trna6Y 1 354244 354332 ON 10 tY-GUA (354244..354282,354297..354332) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.18 Scer_10.trna7R 1 355374 355445 ON 10 tR-UCU (355374..355445) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 Scer_10.trna8D 1 355456 355527 ON 10 tD-GUC (355456..355527) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YJL045W 1 356024 357928 ON 10 YJL045W (356024..357928) Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner YJL044C 0 358300 359676 ON 10 GYP6 complement(358300..359676) GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport YJL043W 1 360130 360903 ON 10 YJL043W (360130..360903) Putative protein of unknown function; YJL043W is a non-essential gene YJL042W 1 361249 365445 ON 10 MHP1 (361249..365445) Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins YJL041W 1 365784 368373 ON 10 NSP1 (365784..365784,365903..368373) FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p) YJL039C 0 368748 373799 ON 10 NUP192 complement(368748..373799) Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Scer_10.trna9D 0 374424 374495 ON 10 tD-GUC complement(374424..374495) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 Scer_10.trna10R 0 374506 374577 ON 10 tR-UCU complement(374506..374577) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YJL038C 0 375115 375774 ON 10 LOH1 complement(375115..375774) Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 YJL037W 1 376662 377336 ON 10 IRC18 (376662..377336) Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Scer_10.trna11V 1 378360 378433 ON 10 tV-AAC (378360..378433) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YJL036W 1 378825 380096 ON 10 SNX4 (378825..380096) Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p YJL035C 0 380249 381001 ON 10 TAD2 complement(380249..381001) Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs YJL034W 1 381327 383375 ON 10 KAR2 (381327..383375) ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p YJL033W 1 383837 386149 ON 10 HCA4 (383837..386149) Putative nucleolar DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis YJL032W 1 386348 386662 OFF 10 YJL032W (386348..386662) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase YJL031C 0 386368 387438 ON 10 BET4 complement(386368..387348,387436..387438) Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p YJL030W 1 387657 388247 ON 10 MAD2 (387657..388247) Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I YJL029C 0 388385 390853 ON 10 VPS53 complement(388385..390853) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Scer_10.trna12M 0 391043 391115 ON 10 tM-CAU complement(391043..391115) Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 70.03 YJL028W 1 391311 391646 OFF 10 YJL028W (391311..391646) Protein of unknown function; may interact with ribosomes, based on co-purification experiments YJL027C 0 391833 392249 OFF 10 YJL027C complement(391833..392249) Putative protein of unknown function YJL026W 1 392404 393603 ON 10 RNR2 (392404..393603) Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits YJL026C-A 0 393066 393287 OFF 10 YJL026C-A complement(393066..393287) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL026W/RNR2 YJL025W 1 393967 395511 ON 10 RRN7 (393967..395511) Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p YJL024C 0 395931 396592 ON 10 APS3 complement(395931..396493,396571..396592) Small subunit of the clathrin-associated adaptor complex AP-3, which is involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function Scer_10.trna13G 0 396726 396796 ON 10 tG-GCC complement(396726..396796) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YJL023C 0 397355 398398 ON 10 PET130 complement(397355..398398) Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL022W 1 398109 398417 OFF 10 YJL022W (398109..398417) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 YJL020C 0 398937 402410 ON 10 BBC1 complement(398937..402410) Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches YJL020W-A 1 400981 401238 OFF 10 YJL020W-A (400981..401238) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL020C/BBC1 YJL019W 1 402897 404945 ON 10 MPS3 (402897..404945) Nuclear envelope protein required for SPB duplication and nuclear fusion; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement YJL016W 1 405588 407273 ON 10 YJL016W (405588..407273) Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species YJL015C 0 407142 407426 OFF 10 YJL015C complement(407142..407426) Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W YJL014W 1 407558 409162 ON 10 CCT3 (407558..409162) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJL013C 0 409493 411040 ON 10 MAD3 complement(409493..411040) Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover YJL012C 0 411234 413399 ON 10 VTC4 complement(411234..413399) Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion YJL011C 0 414285 414770 ON 10 RPC17 complement(414285..414770) RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex Scer_10.trna14K 1 414966 415038 ON 10 tK-CUU (414966..415038) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 Scer_10.trna15W 0 415931 416036 ON 10 tW-CCA complement(415931..415966,416001..416036) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YJL010C 0 417562 419562 ON 10 NOP9 complement(417562..419562) Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain YJL009W 1 419855 420181 OFF 10 YJL009W (419855..420181) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex YJL008C 0 419957 421663 ON 10 CCT8 complement(419957..421663) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJL007C 0 422698 423012 OFF 10 YJL007C complement(422698..423012) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_10.trna16M 1 422937 423009 ON 10 tM-CAU (422937..423009) Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 70.03 YJL006C 0 423138 424109 ON 10 CTK2 complement(423138..424109) Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity Scer_10.trna17L 0 424432 424515 ON 10 tL-UAA complement(424432..424515) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YJL005W 1 425157 431237 ON 10 CYR1 (425157..431237) Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation YJL004C 0 431589 432200 ON 10 SYS1 complement(431589..432200) Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation YJL003W 1 432644 433000 ON 10 COX16 (432644..433000) Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase YJL002C 0 433221 434651 ON 10 OST1 complement(433221..434651) Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins YJL001W 1 435163 435926 ON 10 PRE3 (435163..435227,435344..435926) Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Scer_CEN10 0 436307 436425 ON 10 CEN10 complement(436307..436425) Chromosome X centromere YJR001W 1 436802 438610 ON 10 AVT1 (436802..438610) Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YJR002W 1 438865 440646 ON 10 MPP10 (438865..440646) Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p YJR003C 0 440994 442553 ON 10 YJR003C complement(440994..442553) Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis YJR004C 0 442909 444861 ON 10 SAG1 complement(442909..444861) Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor YJR005W 1 445923 448025 ON 10 APL1 (445923..448025) Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex YJR005C-A 0 448477 448758 ON 10 YJR005C-A complement(448477..448758) Putative protein of unknown function, originally identified as a syntenic homolog of an Ashbya gossypii gene YJR006W 1 449202 450665 ON 10 POL31 (449202..450665) Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair YJR007W 1 451020 451934 ON 10 SUI2 (451020..451934) Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP YJR008W 1 452430 453446 ON 10 MHO1 (452430..453446) Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility YJR009C 0 453683 454681 ON 10 TDH2 complement(453683..454681) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall YJR010W 1 456239 457774 ON 10 MET3 (456239..457774) ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism YJR010C-A 0 458077 458361 ON 10 SPC1 complement(458077..458361) Subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER), homolog of the SPC12 subunit of mammalian signal peptidase complex YJR011C 0 458641 459426 ON 10 YJR011C complement(458641..459426) Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS YJR012C 0 459795 460418 ON 10 YJR012C complement(459795..460418) Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W YJR013W 1 460382 461593 ON 10 GPI14 (460382..461593) Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M YJR014W 1 461829 462425 ON 10 TMA22 (461829..462425) Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1 YJR015W 1 462721 464253 ON 10 YJR015W (462721..464253) Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene YJR016C 0 464451 466208 ON 10 ILV3 complement(464451..466208) Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids YJR017C 0 466521 467033 ON 10 ESS1 complement(466521..467033) Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 YJR018W 1 466786 467148 OFF 10 YJR018W (466786..467148) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR019C 0 467232 468281 ON 10 TES1 complement(467232..468281) Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids YJR020W 1 468001 468333 OFF 10 YJR020W (468001..468333) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR021C 0 468555 469579 ON 10 REC107 complement(468555..469183,469264..469579) Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation YJR022W 1 469784 470113 ON 10 LSM8 (469784..470113) Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA YJR023C 0 469804 470205 OFF 10 YJR023C complement(469804..470205) Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing YJR024C 0 470230 470964 ON 10 MDE1 complement(470230..470964) 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant YJR025C 0 471138 471671 ON 10 BNA1 complement(471138..471671) 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p YJR030C 0 483959 486196 ON 10 YJR030C complement(483959..486196) Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; YJR030C is a non-essential gene YJR031C 0 486586 490812 ON 10 GEA1 complement(486586..490812) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea2p YJR032W 1 491081 492262 ON 10 CPR7 (491081..492262) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity YJR033C 0 492378 496451 ON 10 RAV1 complement(492378..496451) Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate YJR034W 1 496683 497009 ON 10 PET191 (496683..497009) Protein required for assembly of cytochrome c oxidase; exists as an oligomer that is integral to the mitochondrial inner membrane and faces the intermembrane space; contains a twin Cx9C motif YJR035W 1 497355 500612 ON 10 RAD26 (497355..500612) Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein YJR036C 0 500713 503391 ON 10 HUL4 complement(500713..503391) Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex YJR037W 1 503102 503485 OFF 10 YJR037W (503102..503485) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces YJR038C 0 503710 504072 OFF 10 YJR038C complement(503710..504072) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR039W 1 503936 507301 ON 10 YJR039W (503936..507301) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR040W 1 507746 510085 ON 10 GEF1 (507746..510085) Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels YJR041C 0 510239 513763 ON 10 URB2 complement(510239..513763) Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis YJR042W 1 514055 516289 ON 10 NUP85 (514055..516289) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 YJR043C 0 516461 517513 ON 10 POL32 complement(516461..517513) Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Scer_10.trna18M 0 517813 517884 ON 10 tM-CAU complement(517813..517884) Methionine initiator tRNA (tRNA-Met), predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 74.96 YJR044C 0 518763 519185 ON 10 VPS55 complement(518763..519185) Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) YJR045C 0 519638 521602 ON 10 SSC1 complement(519638..521602) Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease YJR046W 1 522048 523862 ON 10 TAH11 (522048..523862) DNA replication licensing factor, required for pre-replication complex assembly Scer_10.trna19S 0 524012 524093 ON 10 tS-AGA complement(524012..524093) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YJR047C 0 524908 525381 ON 10 ANB1 complement(524908..525381) Translation elongation factor eIF-5A, previously thought to function in translation initiation; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions YJR048W 1 526335 526664 ON 10 CYC1 (526335..526664) Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration YJR049C 0 526884 528476 ON 10 UTR1 complement(526884..528476) ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p) YJR050W 1 528697 529404 ON 10 ISY1 (528697..529404) Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles YJR051W 1 529861 531366 ON 10 OSM1 (529861..531366) Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity Scer_10.trna20G 1 531828 531898 ON 10 tG-GCC (531828..531898) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YJR052W 1 532062 533759 ON 10 RAD7 (532062..533759) Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex YJR053W 1 534027 535751 ON 10 BFA1 (534027..535751) Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis YJR054W 1 536056 537549 ON 10 YJR054W (536056..537549) Vacuolar protein of unknown function; potential Cdc28p substrate Scer_10.trna21R 1 538555 538626 ON 10 tR-CCU (538555..538626) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; low abundance tRNA required for growth on nonfermentable carbon sources at high temperatures, for synthesis of heat shock protein Ssc1p, and for Ty1 retrotransposition through the regulation of translational frameshifting; tRNA Type: Arg (R) | Anti-Codon: CCU | Cove Score: 71.23 YJR055W 1 538772 539266 ON 10 HIT1 (538772..539266) Protein of unknown function, required for growth at high temperature Scer_10.trna22D 1 541508 541579 ON 10 tD-GUC (541508..541579) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YJR056C 0 541792 542502 ON 10 YJR056C complement(541792..542502) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Scer_10.trna23Y 0 542956 543044 ON 10 tY-GUA complement(542956..542991,543006..543044) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.81 YJR057W 1 544062 544712 ON 10 CDC8 (544062..544712) Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p YJR058C 0 544732 545175 ON 10 APS2 complement(544732..545175) Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex YJR059W 1 545787 548243 ON 10 PTK2 (545787..548243) Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake YJR060W 1 548759 549814 ON 10 CBF1 (548759..549814) Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation YJR061W 1 550511 553318 ON 10 YJR061W (550511..553318) Putative protein of unknown function; non-essential gene with similarity to Mnn4, a putative membrane protein involved in glycosylation; transcription repressed by Rm101p YJR062C 0 553476 554849 ON 10 NTA1 complement(553476..554849) Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation YJR063W 1 555195 555572 ON 10 RPA12 (555195..555572) RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex YJR064W 1 555914 557602 ON 10 CCT5 (555914..557602) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJR065C 0 557809 559158 ON 10 ARP3 complement(557809..559158) Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity YJR066W 1 559416 566828 ON 10 TOR1 (559416..566828) PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis YJR067C 0 567019 567444 ON 10 YAE1 complement(567019..567444) Protein of unknown function, essential for growth under standard (aerobic) conditions but not under anaerobic conditions YJR068W 1 567643 568704 ON 10 RFC2 (567643..568704) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YJR069C 0 568806 569399 ON 10 HAM1 complement(568806..569399) Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine YJR070C 0 569621 570598 ON 10 LIA1 complement(569621..570598) Deoxyhypusine hydroxylase, a HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning YJR071W 1 570405 570773 OFF 10 YJR071W (570405..570773) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR072C 0 570967 572124 ON 10 NPA3 complement(570967..572124) Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II YJR073C 0 572315 572935 ON 10 OPI3 complement(572315..572935) Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis YJR074W 1 573095 573751 ON 10 MOG1 (573095..573751) Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p YJR075W 1 573981 575171 ON 10 HOC1 (573981..575171) Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele YJR076C 0 575354 576601 ON 10 CDC11 complement(575354..576601) Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells YJR077C 0 577255 578190 ON 10 MIR1 complement(577255..578190) Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated YJR078W 1 578860 580221 ON 10 BNA2 (578860..580221) Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp YJR079W 1 580205 581239 OFF 10 YJR079W (580205..580347,581053..581239) Putative protein of unknown function; mutation results in impaired mitochondrial respiration YJR080C 0 580432 581616 ON 10 AIM24 complement(580432..581616) Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss YJR082C 0 581914 582255 ON 10 EAF6 complement(581914..582255) Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 YJR083C 0 582608 583537 ON 10 ACF4 complement(582608..583537) Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate YJR084W 1 583733 585004 ON 10 YJR084W (583733..585004) Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome YJR085C 0 585120 585437 ON 10 YJR085C complement(585120..585437) Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR086W 1 586068 586400 ON 10 STE18 (586068..586400) G protein gamma subunit, forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling YJR087W 1 586400 586750 OFF 10 YJR087W (586400..586750) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 YJR088C 0 586495 587373 ON 10 EMC2 complement(586495..587373) Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response YJR089W 1 587718 590582 ON 10 BIR1 (587718..590582) Subunit of chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates chromosome segregation; required for chromosome bi-orientation and for spindle assembly checkpoint activation upon reduced sister kinetochore tension YJR090C 0 590872 594327 ON 10 GRR1 complement(590872..594327) F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification YJR091C 0 595061 598336 ON 10 JSN1 complement(595061..598336) Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl YJR092W 1 598735 603078 ON 10 BUD4 (598735..603078) Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p YJR093C 0 603220 604203 ON 10 FIP1 complement(603220..604203) Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p YJR094C 0 604569 605651 ON 10 IME1 complement(604569..605651) Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p YJR094W-A 1 608305 608858 ON 10 RPL43B (608305..608306,608582..608858) Protein component of the large (60S) ribosomal subunit, identical to Rpl43Ap and has similarity to rat L37a ribosomal protein YJR095W 1 609773 610741 ON 10 SFC1 (609773..610741) Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization YJR096W 1 611195 612043 ON 10 YJR096W (611195..612043) Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YJR097W 1 612413 612931 ON 10 JJJ3 (612413..612931) Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 YJR098C 0 613186 615156 ON 10 YJR098C complement(613186..615156) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR099W 1 615576 616286 ON 10 YUH1 (615576..616286) Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p YJR100C 0 616351 617334 ON 10 AIM25 complement(616351..617334) Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Scer_10.trna24L 1 617919 618019 ON 10 tL-UAG (617919..617956,617976..618019) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAG | Cove Score: 61.03 YJR101W 1 618234 619034 ON 10 RSM26 (618234..619034) Mitochondrial ribosomal protein of the small subunit YJR102C 0 619157 619765 ON 10 VPS25 complement(619157..619765) Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome YJR103W 1 620754 622490 ON 10 URA8 (620754..622490) Minor CTP synthase isozyme (see also URA7), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis YJR104C 0 622550 623014 ON 10 SOD1 complement(622550..623014) Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans YJR105W 1 623581 624603 ON 10 ADO1 (623581..624603) Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle YJR106W 1 624838 627015 ON 10 ECM27 (624838..627015) Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p YJR107W 1 627340 628326 ON 10 YJR107W (627340..628326) Putative protein of unknown function; has sequence or structural similarity to lipases Scer_YGOB_Anc_7.495 0 628457 628693 ON 10 Anc_7.495 complement(628457..628693) ANNOTATED BY YGOB - initially created to step over frameshift error in reference genome which later corrected in SGD 2012 release. No frameshift in Liti et al S288c sequence, or in Miura et al S288c cDNAs. YJR108W 1 628712 629083 ON 10 ABM1 (628712..629083) Protein of unknown function, required for normal microtubule organization YJR109C 0 629585 632941 ON 10 CPA2 complement(629585..632941) Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor YJR110W 1 633615 635681 ON 10 YMR1 (633615..635681) Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family YJR111C 0 635855 636706 ON 10 YJR111C complement(635855..636706) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria YJR112W 1 637030 637635 ON 10 NNF1 (637030..637635) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation YJR112W-A 1 637790 638168 ON 10 YJR112W-A (637790..637809,637859..638168) Putative protein of unknown function; identified based on homology to Ashbya gossypii YJR113C 0 638232 638975 ON 10 RSM7 complement(638232..638975) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein YJR114W 1 638659 639051 OFF 10 YJR114W (638659..639051) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSM7/YJR113C YJR115W 1 639942 640451 ON 10 YJR115W (639942..640451) Putative protein of unknown function YJR116W 1 640825 641664 ON 10 TDA4 (640825..641664) Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele YJR117W 1 642007 643368 ON 10 STE24 (642007..643368) Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans YJR118C 0 643490 644101 ON 10 ILM1 complement(643490..644101) Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth YJR119C 0 644304 646490 ON 10 JHD2 complement(644304..646490) JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation YJR120W 1 647126 647476 OFF 10 YJR120W (647126..647476) Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p YJR121W 1 647607 649142 ON 10 ATP2 (647607..649142) Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated YJR122W 1 649776 651269 ON 10 IBA57 (649776..651269) Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system YJR123W 1 651901 652578 ON 10 RPS5 (651901..652578) Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins YJR124C 0 652892 654238 ON 10 YJR124C complement(652892..654238) Putative protein of unknown function; expression induced under calcium shortage YJR125C 0 654737 655963 ON 10 ENT3 complement(654737..655963) Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p YJR126C 0 656254 658689 ON 10 VPS70 complement(656254..658689) Protein of unknown function involved in vacuolar protein sorting YJR127C 0 658917 663059 ON 10 RSF2 complement(658917..663059) Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions YJR128W 1 662921 663280 OFF 10 YJR128W (662921..663280) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Scer_snR3 1 663749 663942 ON 10 SNR3 (663749..663942) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2129, U2133, and U2264 YJR129C 0 664000 665019 ON 10 YJR129C complement(664000..665019) Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR130C 0 665218 667137 ON 10 STR2 complement(665218..667137) Cystathionine gamma-synthase, converts cysteine into cystathionine YJR131W 1 667644 669293 ON 10 MNS1 (667644..669293) Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation YJR132W 1 669522 672668 ON 10 NMD5 (669522..672668) Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog YJR133W 1 672991 673620 ON 10 XPT1 (672991..673620) Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine YJR134C 0 673729 675852 ON 10 SGM1 complement(673729..675852) Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus YJR135C 0 676059 676778 ON 10 MCM22 complement(676059..676778) Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner YJR135W-A 1 676971 677234 ON 10 TIM8 (676971..677234) Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome YJR136C 0 677441 678706 ON 10 TTI2 complement(677441..678706) Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti2p; may interact with Rsm23p; GFP-fusion protein localizes to the cytoplasm YJR137C 0 678957 683285 ON 10 MET5 complement(678957..683285) Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine YJR138W 1 684567 689321 ON 10 IML1 (684567..689321) Subunit of both the SEA (Seh1-associated) and Iml1p complexes; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; localized to either pre-autophagosomal structures (PAS) or non-PAS structures during NNS-induced autophagy; has an N-terminal CDC48-like domain (RANS) required for NNS-induced autophagy and a C-terminal DEP domain YJR139C 0 689445 690524 ON 10 HOM6 complement(689445..690524) Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions YJR140C 0 690750 695696 ON 10 HIR3 complement(690750..695696) Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein YJR140W-A 1 690961 691110 OFF 10 YJR140W-A (690961..691110) Dubious open reading frame; completely overlaps the verified gene HIR3; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YJR141W 1 695906 696949 ON 10 YJR141W (695906..696949) Essential protein of unknown function YJR142W 1 697141 698169 ON 10 YJR142W (697141..698169) Putative protein of unknown function; GST fusion protein is a Dbf2-Mob1 phosphoylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition YJR143C 0 698326 700614 ON 10 PMT4 complement(698326..700614) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals YJR144W 1 700882 701691 ON 10 MGM101 (700882..701691) Protein with a role in mitochondrial DNA recombinational repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage YJR145C 0 702027 703068 ON 10 RPS4A complement(702027..702798,703055..703068) Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein YJR146W 1 703885 704238 OFF 10 YJR146W (703885..704238) Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 YJR147W 1 704196 705272 ON 10 HMS2 (704196..705272) Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant YJR148W 1 705744 706874 ON 10 BAT2 (705744..706874) Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase YJR149W 1 707160 708374 ON 10 YJR149W (707160..708374) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR150C 0 708811 709707 ON 10 DAN1 complement(708811..709707) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YJR151C 0 712255 715740 ON 10 DAN4 complement(712255..715740) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YJR151W-A 1 717580 717630 OFF 10 YJR151W-A (717580..717630) Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational "guilt by association" analysis YJR152W 1 719666 721297 ON 10 DAL5 (719666..721297) Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression YJR153W 1 722815 723900 ON 10 PGU1 (722815..723900) Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins YJR154W 1 725784 726824 ON 10 YJR154W (725784..726824) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR155W 1 727405 728271 ON 10 AAD10 (727405..728271) Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YJR156C 0 728574 729596 ON 10 THI11 complement(728574..729596) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YJR157W 1 730515 730877 OFF 10 YJR157W (730515..730877) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR158W 1 732440 734143 ON 10 HXT16 (732440..734143) Protein of unknown function with similarity to hexose transporter family members, expression is repressed by high levels of glucose YJR159W 1 736044 737117 ON 10 SOR1 (736044..737117) Sorbitol dehydrogenase; expression is induced in the presence of sorbitol or xylose YJR160C 0 738008 739816 ON 10 MPH3 complement(738008..739816) Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication YJR161C 0 742848 743999 ON 10 COS5 complement(742848..743999) Protein of unknown function, member the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YJR162C 0 744911 745261 OFF 10 YJR162C complement(744911..745261) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL225W 1 451 798 OFF 11 YKL225W (451..798) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL224C 0 1810 2181 ON 11 PAU16 complement(1810..2181) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YKL223W 1 2389 2721 OFF 11 YKL223W (2389..2721) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL222C 0 3503 5620 ON 11 YKL222C complement(3503..5620) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine YKL221W 1 6107 7528 ON 11 MCH2 (6107..7528) Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport YKL220C 0 9091 11226 ON 11 FRE2 complement(9091..11226) Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels YKL219W 1 14485 15708 ON 11 COS9 (14485..15708) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YKL218C 0 17359 18339 ON 11 SRY1 complement(17359..18339) 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity YKL217W 1 22234 24084 ON 11 JEN1 (22234..24084) Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose YKL216W 1 25215 26159 ON 11 URA1 (25215..26159) Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid YKL215C 0 26827 30687 ON 11 OXP1 complement(26827..30687) 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL214C 0 31082 31693 ON 11 YRA2 complement(31082..31693) Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus YKL213C 0 31960 34107 ON 11 DOA1 complement(31960..34107) WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase YKL212W 1 34543 36414 ON 11 SAC1 (34543..36414) Phosphatidylinositol phosphate (PtdInsP) phosphatase involved in hydrolysis of PtdIns[4]P; transmembrane protein localizes to ER and Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance YKL211C 0 36699 38153 ON 11 TRP3 complement(36699..38153) Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Scer_snR64 1 38811 38911 ON 11 SNR64 (38811..38911) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position C2337 YKL210W 1 39163 42237 ON 11 UBA1 (39163..42237) Ubiquitin activating enzyme (E1), involved in ubiquitin-mediated protein degradation and essential for viability YKL209C 0 42423 46295 ON 11 STE6 complement(42423..46295) Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells Scer_11.trna1T 0 46735 46806 ON 11 tT-CGU complement(46735..46806) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; correct folding and efficient aminoacylation require Lhp1p; tRNA Type: Thr (T) | Anti-Codon: CGU | Cove Score: 75.96 YKL208W 1 47157 47972 ON 11 CBT1 (47157..47972) Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p YKL207W 1 48194 48955 ON 11 EMC3 (48194..48955) Member of a transmembrane complex required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response YKL206C 0 49006 49809 ON 11 ADD66 complement(49006..49809) Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly YKL205W 1 50051 53353 ON 11 LOS1 (50051..53353) Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm YKL204W 1 53704 55602 ON 11 EAP1 (53704..55602) eIF4E-associated protein, competes with eIF4G for binding to eIF4E; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade YKL203C 0 55935 63359 ON 11 TOR2 complement(55935..63359) PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis YKL202W 1 63821 64402 OFF 11 YKL202W (63821..64402) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL201C 0 63930 67466 ON 11 MNN4 complement(63930..67466) Putative positive regulator of mannosylphosphate transferase (Mnn6p), involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases YKL198C 0 68232 70220 ON 11 PTK1 complement(68232..70220) Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein YKL197C 0 70734 73865 ON 11 PEX1 complement(70734..73865) AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Scer_11.trna2N 1 74624 74697 ON 11 tN-GUU (74624..74697) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YKL196C 0 74932 75534 ON 11 YKT6 complement(74932..75534) Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus YKL195W 1 75821 77032 ON 11 MIA40 (75821..77032) Essential protein of the mitochondrial intermembrane space (IMS); promotes retention of newly imported proteins; may do so by stabilizing client protein folding as part of a disulfide relay system or transferring metal to client proteins YKL194C 0 77258 78646 ON 11 MST1 complement(77258..78646) Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro YKL193C 0 78866 79882 ON 11 SDS22 complement(78866..79882) Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function YKL192C 0 80160 80537 ON 11 ACP1 complement(80160..80537) Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p YKL191W 1 81035 82639 ON 11 DPH2 (81035..82639) Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p YKL190W 1 82947 83550 ON 11 CNB1 (82947..82998,83075..83550) Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 Scer_11.trna3L 1 84208 84291 ON 11 tL-UAA (84208..84291) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YKL189W 1 84704 85903 ON 11 HYM1 (84704..85903) Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response YKL188C 0 86225 88786 ON 11 PXA2 complement(86225..88786) Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder YKL187C 0 89284 91536 ON 11 YKL187C complement(89284..91536) Putative protein of unknown function; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies YKL186C 0 92744 93298 ON 11 MTR2 complement(92744..93298) mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA YKL185W 1 94499 96265 ON 11 ASH1 (94499..96265) Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate YKL184W 1 96757 98157 ON 11 SPE1 (96757..98157) Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation YKL183C-A 0 98395 98607 OFF 11 YKL183C-A complement(98395..98607) Putative protein of unknown function; identified by fungal homology and RT-PCR YKL183W 1 98721 99641 ON 11 LOT5 (98721..99641) Protein of unknown function; gene expression increases in cultures shifted to a lower temperature YKL182W 1 100671 106826 ON 11 FAS1 (100671..106826) Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities YKL181W 1 107316 108599 ON 11 PRS1 (107316..108599) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YKL180W 1 109269 110129 ON 11 RPL17A (109269..109577,109884..110129) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex) YKL179C 0 110464 112503 ON 11 COY1 complement(110464..112503) Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function YKL178C 0 113215 114627 ON 11 STE3 complement(113215..114627) Receptor for a factor pheromone, couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR YKL177W 1 114356 114694 OFF 11 YKL177W (114356..114694) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3 YKL176C 0 115500 117986 ON 11 LST4 complement(115500..117986) Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface YKL175W 1 118793 120304 ON 11 ZRT3 (118793..120304) Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency YKL174C 0 120380 122236 ON 11 TPO5 complement(120380..122236) Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles YKL173W 1 122517 125543 ON 11 SNU114 (122517..125543) GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 YKL172W 1 125759 127042 ON 11 EBP2 (125759..127042) Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering YKL171W 1 127475 130261 ON 11 NNK1 (127475..130261) Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm YKL170W 1 130634 131050 ON 11 MRPL38 (130634..131050) Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels YKL169C 0 130685 131068 OFF 11 YKL169C complement(130685..131068) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 YKL168C 0 131288 133462 ON 11 KKQ8 complement(131288..133462) Putative serine/threonine protein kinase with unknown cellular role YKL167C 0 133721 134134 ON 11 MRP49 complement(133721..134134) Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation YKL166C 0 134509 135705 ON 11 TPK3 complement(134509..135705) cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase YKL165C-A 0 135790 136023 OFF 11 YKL165C-A complement(135790..136023) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL165C 0 137932 140691 ON 11 MCD4 complement(137932..140691) Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Scer_11.trna4E 0 141018 141089 ON 11 tE-UUC complement(141018..141089) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YKL164C 0 141794 142819 ON 11 PIR1 complement(141794..142819) O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle YKL163W 1 144401 145378 ON 11 PIR3 (144401..145378) O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway YKL162C-A 0 145922 146074 OFF 11 YKL162C-A complement(145922..146074) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL162C 0 147630 148838 ON 11 YKL162C complement(147630..148838) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YKL161C 0 149386 150687 ON 11 KDX1 complement(149386..150687) Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p YKL160W 1 153269 153706 ON 11 ELF1 (153269..153706) Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression YKL159C 0 153816 154451 ON 11 RCN1 complement(153816..154451) Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region YKL157W 1 154991 158232 ON 11 APE2 (154991..155271,155655..158232) Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W YKL156W 1 158613 159212 ON 11 RPS27A (158613..158615,158967..159212) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein YKL156C-A 0 158975 159091 OFF 11 YKL156C-A complement(158975..159091) Dubious open reading frame unlikely to encode a protein; completely overlaps the verified gene RPS27A; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKL155C 0 159456 161342 ON 11 RSM22 complement(159456..161342) Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase YKL154W 1 161601 162335 ON 11 SRP102 (161601..162335) Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Scer_11.trna5R 0 162487 162558 ON 11 tR-UCU complement(162487..162558) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YKL153W 1 163600 164109 OFF 11 YKL153W (163600..164109) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant YKL152C 0 163642 164385 ON 11 GPM1 complement(163642..164385) Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis YKL151C 0 164917 165930 ON 11 YKL151C complement(164917..165930) NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL150W 1 166544 167452 ON 11 MCR1 (166544..167452) Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis YKL149C 0 167612 168829 ON 11 DBR1 complement(167612..168829) RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition YKL148C 0 169207 171129 ON 11 SDH1 complement(169207..171129) Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain YKL147C 0 171551 172168 OFF 11 YKL147C complement(171551..172168) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 YKL146W 1 171783 173861 ON 11 AVT3 (171783..173861) Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YKL145W 1 174213 175616 ON 11 RPT1 (174213..175616) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance YKL145W-A 1 174958 175050 OFF 11 YKL145W-A (174958..175050) Dubious open reading frame, unlikely to encode a protein; completely overlaps the verified essential gene RPT1; identified by expression profiling and mass spectrometry YKL144C 0 175843 176481 ON 11 RPC25 complement(175843..176481) RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p YKL143W 1 176781 178172 ON 11 LTV1 (176781..178172) Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature YKL142W 1 178515 179174 ON 11 MRP8 (178515..179174) Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; originally thought to be a mitochondrial ribosomal protein based on sequence analysis YKL141W 1 179667 180263 ON 11 SDH3 (179667..180263) Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex YKL140W 1 180779 182425 ON 11 TGL1 (180779..182425) Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes YKL139W 1 182958 184544 ON 11 CTK1 (182958..184544) Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 YKL138C-A 0 184803 185012 ON 11 HSK3 complement(184803..185012) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YKL138C 0 185286 185681 ON 11 MRPL31 complement(185286..185681) Mitochondrial ribosomal protein of the large subunit YKL137W 1 185957 186292 ON 11 CMC1 (185957..186292) Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif YKL136W 1 186416 186814 OFF 11 YKL136W (186416..186814) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C YKL135C 0 186460 188640 ON 11 APL2 complement(186460..188640) Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting YKL134C 0 189124 191442 ON 11 OCT1 complement(189124..191442) Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis YKL133C 0 191679 193070 ON 11 YKL133C complement(191679..193070) Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) YKL132C 0 193574 194866 ON 11 RMA1 complement(193574..194866) Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKL131W 1 194478 194999 OFF 11 YKL131W (194478..194999) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL130C 0 195288 196028 ON 11 SHE2 complement(195288..196028) RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud YKL129C 0 196349 200167 ON 11 MYO3 complement(196349..200167) One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization YKL128C 0 200884 201771 ON 11 PMU1 complement(200884..201771) Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Scer_11.trna6K 1 202999 203071 ON 11 tK-CUU (202999..203071) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YKL127W 1 203541 205253 ON 11 PGM1 (203541..205253) Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism YKL126W 1 205707 207749 ON 11 YPK1 (205707..207749) Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; also inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK YKL125W 1 208247 210130 ON 11 RRN3 (208247..210130) Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo YKL124W 1 210593 212332 ON 11 SSH4 (210593..212332) Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases YKL123W 1 212043 212423 OFF 11 YKL123W (212043..212423) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 YKL122C 0 212495 212998 ON 11 SRP21 complement(212495..212998) Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm YKL121W 1 214142 216700 ON 11 DGR2 (214142..216700) Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds YKL120W 1 217344 218318 ON 11 OAC1 (217344..218318) Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family YKL119C 0 218570 219217 ON 11 VPH2 complement(218570..219217) Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) YKL118W 1 219126 219437 OFF 11 YKL118W (219126..219437) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Scer_11.trna7A 1 219895 219967 ON 11 tA-AGC (219895..219967) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YKL117W 1 220324 220974 ON 11 SBA1 (220324..220974) Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins and like p23 can regulate telomerase activity YKL116C 0 221344 222900 ON 11 PRR1 complement(221344..222900) Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor YKL115C 0 222893 223285 OFF 11 YKL115C complement(222893..223285) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 YKL114C 0 223352 224455 ON 11 APN1 complement(223352..224455) Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine YKL113C 0 224727 225875 ON 11 RAD27 complement(224727..225875) 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family YKL112W 1 226570 228765 ON 11 ABF1 (226570..228765) DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair YKL111C 0 228464 228799 OFF 11 YKL111C complement(228464..228799) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 YKL110C 0 228939 229880 ON 11 KTI12 complement(228939..229880) Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p YKL109W 1 232227 233891 ON 11 HAP4 (232227..233891) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex YKL108W 1 234426 235787 ON 11 SLD2 (234426..235787) Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint YKL107W 1 236141 237070 ON 11 YKL107W (236141..237070) Putative protein of unknown function; proposed to be a palmitoylated membrane protein YKL106C-A 0 237147 237266 OFF 11 YKL106C-A complement(237147..237266) Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi YKL106W 1 237536 238891 ON 11 AAT1 (237536..238891) Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis YKL105C 0 239185 242583 ON 11 YKL105C complement(239185..242583) Putative protein of unknown function; similar to A. gossypii SEG gene which is important for stabilizing eisosomes YKL104C 0 243220 245373 ON 11 GFA1 complement(243220..245373) Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis YKL103C 0 246138 247682 ON 11 APE1 complement(246138..247682) Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway YKL102C 0 248062 248367 OFF 11 YKL102C complement(248062..248367) Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site YKL101W 1 248920 253476 ON 11 HSL1 (248920..253476) Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p YKL100C 0 253697 255460 ON 11 YKL100C complement(253697..255460) Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene YKL100W-A 1 254159 254248 OFF 11 YKL100W-A (254159..254248) Putative protein of unknown function; identified by expression profiling and mass spectrometry YKL099C 0 255720 256472 ON 11 UTP11 complement(255720..256472) Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit YKL098W 1 256770 257843 ON 11 MTC2 (256770..257843) Protein of unknown function; mtc2 is synthetically sick with cdc13-1 YKL097C 0 258503 258913 OFF 11 YKL097C complement(258503..258913) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species YKL096C-B 0 259073 259222 OFF 11 YKL096C-B complement(259073..259222) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKL096W-A 1 259253 259531 ON 11 CWP2 (259253..259531) Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored YKL096W 1 261132 261851 ON 11 CWP1 (261132..261851) Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance YKL095W 1 262277 263113 ON 11 YJU2 (262277..263113) Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing YKL094W 1 263349 264290 ON 11 YJU3 (263349..264290) Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family YKL093W 1 264789 265808 ON 11 MBR1 (264789..265808) Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants YKL092C 0 266145 269459 ON 11 BUD2 complement(266145..269459) GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types YKL091C 0 269718 270650 ON 11 YKL091C complement(269718..270650) Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study YKL090W 1 271878 273209 ON 11 CUE2 (271878..273209) Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination YKL089W 1 273394 275043 ON 11 MIF2 (273394..275043) Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p YKL088W 1 275283 276998 ON 11 CAB3 (275283..276998) Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC YKL087C 0 277189 277863 ON 11 CYT2 complement(277189..277863) Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 YKL086W 1 278281 278664 ON 11 SRX1 (278281..278664) Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes YKL085W 1 279123 280127 ON 11 MDH1 (279123..280127) Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated YKL084W 1 280509 280859 ON 11 HOT13 (280509..280859) Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc YKL083W 1 280921 281535 OFF 11 YKL083W (280921..281535) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 YKL082C 0 281025 282329 ON 11 RRP14 complement(281025..282329) Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family YKL081W 1 282891 284455 ON 11 TEF4 (282891..283095,283422..284455) Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Scer_snR38 1 283185 283279 OFF 11 SNR38 (283185..283279) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position G2815 YKL080W 1 285030 286208 ON 11 VMA5 (285030..286208) Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane YKL079W 1 286603 288573 ON 11 SMY1 (286603..288573) Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins YKL078W 1 288845 291052 ON 11 DHR2 (288845..291052) Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis YKL077W 1 291453 292631 ON 11 YKL077W (291453..292631) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YKL076C 0 292838 293221 OFF 11 PSY1 complement(292838..293221) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C YKL075C 0 292956 294308 ON 11 YKL075C complement(292956..294308) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin YKL074C 0 294610 296193 ON 11 MUD2 complement(294610..296193) Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 YKL073W 1 296430 299075 ON 11 LHS1 (296430..299075) Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway YKL072W 1 299582 301882 ON 11 STB6 (299582..301882) Protein that binds Sin3p in a two-hybrid assay Scer_11.trna8W 1 302918 303023 ON 11 tW-CCA (302918..302953,302988..303023) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YKL071W 1 305114 305884 ON 11 YKL071W (305114..305884) Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL070W 1 306211 306720 ON 11 YKL070W (306211..306720) Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKL069W 1 307285 307827 ON 11 YKL069W (307285..307827) Methionine-R-sulfoxide reductase, reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress Scer_11.trna9V 0 308144 308217 ON 11 tV-AAC complement(308144..308217) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YKL068W-A 1 309206 309442 ON 11 YKL068W-A (309206..309442) Putative protein of unknown function; identified by homology to Ashbya gossypii YKL068W 1 310199 313078 ON 11 NUP100 (310199..313078) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Scer_11.trna10H 0 313401 313472 ON 11 tH-GUG complement(313401..313472) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YKL067W 1 314812 315273 ON 11 YNK1 (314812..315273) Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate YKL066W 1 315281 315724 OFF 11 YKL066W (315281..315724) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 YKL065W-A 1 315954 316175 ON 11 YKL065W-A (315954..316175) Putative protein of unknown function YKL065C 0 316437 317057 ON 11 YET1 complement(316437..317057) Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein YKL064W 1 317764 320673 ON 11 MNR2 (317764..320673) Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations YKL063C 0 321015 321518 ON 11 YKL063C complement(321015..321518) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi YKL062W 1 323228 325120 ON 11 MSN4 (323228..325120) Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression YKL061W 1 325771 326112 ON 11 BLI1 (325771..326112) Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome YKL060C 0 326408 327487 ON 11 FBA1 complement(326408..327487) Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress YKL059C 0 328118 329443 ON 11 MPE1 complement(328118..329443) Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif YKL058W 1 330166 330534 ON 11 TOA2 (330166..330534) TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA YKL057C 0 330856 333969 ON 11 NUP120 complement(330856..333969) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 YKL056C 0 334412 334915 ON 11 TMA19 complement(334412..334915) Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress YKL055C 0 335321 336157 ON 11 OAR1 complement(335321..336157) Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p YKL054C 0 336537 338753 ON 11 DEF1 complement(336537..338753) RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis YKL053C-A 0 339182 339442 ON 11 MDM35 complement(339182..339442) Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs YKL053W 1 339572 339946 OFF 11 YKL053W (339572..339946) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 YKL052C 0 339668 340546 ON 11 ASK1 complement(339668..340546) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases YKL051W 1 341312 342373 ON 11 SFK1 (341312..342373) Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane YKL050C 0 342852 345620 ON 11 YKL050C complement(342852..345620) Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p YKL049C 0 346075 346764 ON 11 CSE4 complement(346075..346764) Centromere protein that resembles histone H3, required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p YKL048C 0 347214 349136 ON 11 ELM1 complement(347214..349136) Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring YKL047W 1 349463 351013 ON 11 YKL047W (349463..351013) Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL046C 0 351275 352624 ON 11 DCW1 complement(351275..352624) Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p YKL045W 1 353493 355079 ON 11 PRI2 (353493..355079) Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair YKL044W 1 356322 356642 OFF 11 YKL044W (356322..356642) Protein of unknown function; mRNA identified as translated by ribosome profiling data YKL043W 1 356748 357848 ON 11 PHD1 (356748..357848) Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate YKL042W 1 358475 359566 ON 11 SPC42 (358475..359566) Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane YKL041W 1 360143 360817 ON 11 VPS24 (360143..360817) One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway YKL040C 0 361058 361828 ON 11 NFU1 complement(361058..361828) Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria YKL039W 1 362622 364193 ON 11 PTM1 (362622..364193) Protein of unknown function, copurifies with late Golgi vesicles containing the v-SNARE Tlg2p Scer_snR69 1 364776 364876 ON 11 SNR69 (364776..364876) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position C2948 YKL038W 1 365605 369117 ON 11 RGT1 (365605..369117) Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor YKL037W 1 369365 369721 OFF 11 AIM26 (369365..369721) Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT YKL036C 0 369528 369920 OFF 11 YKL036C complement(369528..369920) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W YKL035W 1 369891 371390 ON 11 UGP1 (369891..371390) UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p YKL034W 1 371829 374105 ON 11 TUL1 (371829..374105) Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes YKL033W-A 1 374505 375215 ON 11 YKL033W-A (374505..375215) Putative protein of unknown function; similar to uncharacterized proteins from other fungi YKL033W 1 375456 378572 ON 11 TTI1 (375456..378572) Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Scer_11.trna11V 1 379680 379753 ON 11 tV-AAC (379680..379753) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YKL032C 0 380065 381858 ON 11 IXR1 complement(380065..381858) Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b YKL031W 1 382072 382485 OFF 11 YKL031W (382072..382485) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species YKL030W 1 382498 383103 OFF 11 YKL030W (382498..383103) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 YKL029C 0 382716 384725 ON 11 MAE1 complement(382716..384725) Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids YKL028W 1 385758 387206 ON 11 TFA1 (385758..387206) TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening YKL027W 1 387562 388905 ON 11 YKL027W (387562..388905) Protein of unknown function, localized to the mitochondrial outer membrane YKL026C 0 389380 389883 ON 11 GPX1 complement(389380..389883) Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress YKL025C 0 390240 392279 ON 11 PAN3 complement(390240..392279) Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes YKL024C 0 392526 393140 ON 11 URA6 complement(392526..393140) Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) YKL023C-A 0 393146 393373 ON 11 YKL023C-A complement(393146..393373) Putative protein of unknown function YKL023W 1 393721 394554 ON 11 YKL023W (393721..394554) Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL022C 0 394726 397248 ON 11 CDC16 complement(394726..397248) Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation YKL021C 0 397344 398750 ON 11 MAK11 complement(397344..398750) Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats YKL020C 0 398832 402080 ON 11 SPT23 complement(398832..402080) ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting YKL019W 1 402568 403518 ON 11 RAM2 (402568..403518) Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor YKL018C-A 0 403580 403879 ON 11 YKL018C-A complement(403580..403879) Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL018W 1 404102 405091 ON 11 SWD2 (404102..405091) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination YKL017C 0 405190 407241 ON 11 HCS1 complement(405190..407241) Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities YKL016C 0 407465 407989 ON 11 ATP7 complement(407465..407989) Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YKL015W 1 408544 411483 ON 11 PUT3 (408544..411483) Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain YKL014C 0 411619 416913 ON 11 URB1 complement(411619..416913) Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit YKL013C 0 417508 418023 ON 11 ARC19 complement(417508..418023) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YKL012W 1 418310 420061 ON 11 PRP40 (418310..420061) U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex YKL011C 0 420155 421216 ON 11 CCE1 complement(420155..421216) Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA YKL010C 0 421424 425875 ON 11 UFD4 complement(421424..425875) Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins YKL009W 1 426242 426952 ON 11 MRT4 (426242..426952) Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus YKL008C 0 427295 428551 ON 11 LAC1 complement(427295..428551) Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p YKL007W 1 429302 430108 ON 11 CAP1 (429302..430108) Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches YKL006C-A 0 430198 430632 ON 11 SFT1 complement(430198..430455,430597..430632) Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Scer_snR87 0 431030 431138 ON 11 SNR87 complement(431030..431138) C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position A436 YKL006W 1 431906 432720 ON 11 RPL14A (431906..432034,432433..432720) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth YKL005C 0 433093 434877 ON 11 BYE1 complement(433093..434877) Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit YKL004W 1 435580 436785 ON 11 AUR1 (435580..436785) Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance YKL003C 0 437097 437492 ON 11 MRP17 complement(437097..437492) Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator YKL002W 1 437778 438544 ON 11 DID4 (437778..437837,437906..438544) Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis YKL001C 0 438777 439385 ON 11 MET14 complement(438777..439385) Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Scer_CEN11 0 440129 440246 ON 11 CEN11 complement(440129..440246) Chromosome XI centromere YKR001C 0 440608 442722 ON 11 VPS1 complement(440608..442722) Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis YKR002W 1 443232 444938 ON 11 PAP1 (443232..444938) Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping YKR003W 1 445381 446727 ON 11 OSH6 (445381..446727) Member of an oxysterol-binding protein family with overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuole fusion YKR004C 0 446799 448036 ON 11 ECM9 complement(446799..447706,447811..448036) Non-essential protein of unknown function YKR005C 0 448521 450167 ON 11 YKR005C complement(448521..449951,450021..450167) Putative protein of unknown function YKR006C 0 450423 451217 ON 11 MRPL13 complement(450423..451217) Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation YKR007W 1 451434 451988 ON 11 MEH1 (451434..451988) Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification YKR008W 1 452201 454078 ON 11 RSC4 (452201..454078) Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p YKR009C 0 454352 457054 ON 11 FOX2 complement(454352..457054) Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities Scer_11.trna12L 1 458557 458670 ON 11 tL-CAA (458557..458594,458627..458670) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YKR010C 0 458924 461239 ON 11 TOF2 complement(458924..461239) Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site YKR011C 0 461635 462696 ON 11 YKR011C complement(461635..462696) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YKR012C 0 463739 464116 OFF 11 YKR012C complement(463739..464116) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 YKR013W 1 463959 464948 ON 11 PRY2 (463959..464948) Protein of unknown function YKR014C 0 465367 466071 ON 11 YPT52 complement(465367..466071) Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis YKR015C 0 466961 468667 ON 11 YKR015C complement(466961..468667) Putative protein of unknown function YKR016W 1 469717 471339 ON 11 FCJ1 (469717..471339) Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane YKR017C 0 471694 473349 ON 11 HEL1 complement(471694..473349) Putative protein of unknown function; contains a RING finger motif YKR018C 0 473723 475900 ON 11 YKR018C complement(473723..475900) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YKR019C 0 476216 478063 ON 11 IRS4 complement(476216..478063) EH domain-containing protein involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS YKR020W 1 478338 478832 ON 11 VPS51 (478338..478832) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p YKR021W 1 479234 481981 ON 11 ALY1 (479234..481981) Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets YKR022C 0 482171 483139 ON 11 NTR2 complement(482171..483139) Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly YKR023W 1 483419 485011 ON 11 YKR023W (483419..485011) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR024C 0 485144 487372 ON 11 DBP7 complement(485144..487372) Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions YKR025W 1 487770 488618 ON 11 RPC37 (487770..488618) RNA polymerase III subunit C37 YKR026C 0 488738 489655 ON 11 GCN3 complement(488738..489655) Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Scer_11.trna13R 1 490968 491040 ON 11 tR-ACG (490968..491040) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 71.46 YKR027W 1 491364 493661 ON 11 BCH2 (491364..493661) Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane YKR028W 1 494257 497358 ON 11 SAP190 (494257..497358) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p YKR029C 0 497578 499833 ON 11 SET3 complement(497578..499833) Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains YKR030W 1 500282 501103 ON 11 GMH1 (500282..501103) Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting YKR031C 0 501344 506395 ON 11 SPO14 complement(501344..506395) Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions YKR032W 1 506517 506831 OFF 11 YKR032W (506517..506831) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKR033C 0 506879 507304 OFF 11 YKR033C complement(506879..507304) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 YKR034W 1 506898 507707 ON 11 DAL80 (506898..507707) Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p YKR035C 0 507920 508561 OFF 11 OPI8 complement(507920..508561) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A YKR035W-A 1 507939 508553 ON 11 DID2 (507939..508553) Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors YKR036C 0 508702 510633 ON 11 CAF4 complement(508702..510633) WD40 repeat-containing protein associated with the CCR4-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission YKR037C 0 510910 511797 ON 11 SPC34 complement(510910..511797) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body YKR038C 0 511999 513159 ON 11 KAE1 complement(511999..513159) Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Scer_11.trna14D 0 513332 513403 ON 11 tD-GUC complement(513332..513403) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YKR039W 1 515063 516871 ON 11 GAP1 (515063..516871) General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed Scer_11.trna15A 1 517988 518060 ON 11 tA-AGC (517988..518060) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YKR040C 0 518063 518566 OFF 11 YKR040C complement(518063..518566) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W YKR041W 1 518198 518950 ON 11 YKR041W (518198..518950) Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking YKR042W 1 519527 520624 ON 11 UTH1 (519527..520624) Mitochondrial outer membrane and cell wall localized SUN family member involved in cell wall biogenesis and required for mitochondrial autophagy; involved in the oxidative stress response, life span during starvation, and cell death YKR043C 0 520897 521712 ON 11 SHB17 complement(520897..521712) Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus YKR044W 1 522015 523346 ON 11 UIP5 (522015..523346) Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates YKR045C 0 523418 523969 ON 11 YKR045C complement(523418..523969) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YKR046C 0 524223 525074 ON 11 PET10 complement(524223..525074) Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange YKR047W 1 525257 525562 OFF 11 YKR047W (525257..525562) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 YKR048C 0 525387 526640 ON 11 NAP1 complement(525387..526640) Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2 YKR049C 0 526830 527231 ON 11 FMP46 complement(526830..527231) Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR050W 1 527815 530484 ON 11 TRK2 (527815..530484) Component of the Trk1p-Trk2p potassium transport system YKR051W 1 530946 532202 ON 11 YKR051W (530946..532202) Putative protein of unknown function YKR052C 0 532550 533464 ON 11 MRS4 complement(532550..533464) Iron transporter that mediates Fe2+ transport across the inner mitochondrial membrane; mitochondrial carrier family member, similar to and functionally redundant with Mrs3p; active under low-iron conditions; may transport other cations YKR053C 0 534067 535281 ON 11 YSR3 complement(534067..535281) Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p YKR054C 0 535647 547925 ON 11 DYN1 complement(535647..547925) Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p YKR055W 1 548216 549091 ON 11 RHO4 (548216..549091) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity YKR056W 1 549448 551367 ON 11 TRM2 (549448..551367) tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair YKR057W 1 551657 552242 ON 11 RPS21A (551657..551680,552003..552242) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Bp and has similarity to rat S21 ribosomal protein YKR058W 1 552770 554620 ON 11 GLG1 (552770..554620) Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin YKR059W 1 554987 556174 ON 11 TIF1 (554987..556174) Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G YKR060W 1 556518 557342 ON 11 UTP30 (556518..557342) Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit YKR061W 1 557677 558954 ON 11 KTR2 (557677..558954) Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Scer_snR42 0 559016 559366 ON 11 SNR42 complement(559016..559366) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2975 YKR062W 1 559666 560652 ON 11 TFA2 (559666..560652) TFIIE small subunit, involved in RNA polymerase II transcription initiation YKR063C 0 560789 562297 ON 11 LAS1 complement(560789..562297) Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability YKR064W 1 562547 565138 ON 11 OAF3 (562547..565138) Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR065C 0 565299 565892 ON 11 PAM17 complement(565299..565892) Constituent of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 complex YKR066C 0 566113 567198 ON 11 CCP1 complement(566113..567198) Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress YKR067W 1 567918 570149 ON 11 GPT2 (567918..570149) Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis YKR068C 0 570328 570909 ON 11 BET3 complement(570328..570909) Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex YKR069W 1 571612 573393 ON 11 MET1 (571612..573393) S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis YKR070W 1 573574 574632 ON 11 YKR070W (573574..574632) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR071C 0 574934 575980 ON 11 DRE2 complement(574934..575980) Conserved component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18p; ortholog of human Ciapin1 YKR072C 0 576435 578123 ON 11 SIS2 complement(576435..578123) Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis YKR073C 0 578187 578507 OFF 11 YKR073C complement(578187..578507) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Scer_11.trna16K 1 578965 579060 ON 11 tK-UUU (578965..579001,579025..579060) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YKR074W 1 579209 579676 ON 11 AIM29 (579209..579676) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss YKR075C 0 579827 580750 ON 11 YKR075C complement(579827..580750) Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YKR075W-A 1 580212 580481 OFF 11 YKR075W-A (580212..580481) Dubious open reading frame unlikely to encode a protein; completely overlaps the uncharacterized gene YKR075C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKR076W 1 582283 583395 ON 11 ECM4 (582283..583395) Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKR077W 1 583653 584744 ON 11 MSA2 (583653..584744) Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation YKR078W 1 584952 586709 ON 11 YKR078W (584952..586709) Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) YKR079C 0 586789 589305 ON 11 TRZ1 complement(586789..589305) tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 YKR080W 1 590395 591357 ON 11 MTD1 (590395..591357) NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline YKR081C 0 591506 592540 ON 11 RPF2 complement(591506..592540) Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles YKR082W 1 592825 596298 ON 11 NUP133 (592825..596298) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 YKR083C 0 596421 596822 ON 11 DAD2 complement(596421..596822) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YKR084C 0 597055 598890 ON 11 HBS1 complement(597055..598890) GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay YKR085C 0 599094 599681 ON 11 MRPL20 complement(599094..599681) Mitochondrial ribosomal protein of the large subunit YKR086W 1 599857 603072 ON 11 PRP16 (599857..603072) DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step YKR087C 0 603195 604232 ON 11 OMA1 complement(603195..604232) Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes YKR088C 0 604405 605418 ON 11 TVP38 complement(604405..605418) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern YKR089C 0 605633 608365 ON 11 TGL4 complement(605633..608365) Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p YKR090W 1 608946 611066 ON 11 PXL1 (608946..611066) LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin YKR091W 1 611525 612265 ON 11 SRL3 (611525..612265) Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate YKR092C 0 612665 613885 ON 11 SRP40 complement(612665..613885) Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 YKR093W 1 615730 617535 ON 11 PTR2 (615730..617535) Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p YKR094C 0 617996 618750 ON 11 RPL40B complement(617996..618374,618743..618750) Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes YKR095W 1 619805 625432 ON 11 MLP1 (619805..625432) Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs YKR095W-A 1 625864 626205 ON 11 PCC1 (625864..625901,625977..626205) Component of the EKC/KEOPS protein complex with Kae1p, Gon7p, Bud32p, and Cgi121p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription YKR096W 1 626793 630380 ON 11 YKR096W (626793..630380) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain YKR097W 1 631152 632801 ON 11 PCK1 (631152..632801) Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol YKR098C 0 633026 635179 ON 11 UBP11 complement(633026..635179) Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins YKR099W 1 635851 638286 ON 11 BAS1 (635851..638286) Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes YKR100C 0 638901 639968 ON 11 SKG1 complement(638901..639968) Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell YKR101W 1 640540 642504 ON 11 SIR1 (640540..642504) Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin YKR102W 1 646356 649865 ON 11 FLO10 (646356..649865) Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth YKR103W 1 653080 656736 ON 11 NFT1 (653080..656736) Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. YKR104W 1 656836 657756 ON 11 YKR104W (656836..657756) Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds YKR105C 0 658716 660464 ON 11 VBA5 complement(658716..660464) Putative transporter of the Major Facilitator Superfamily (MFS); proposed role as a basic amino acid permease based on phylogeny YKR106W 1 661442 663289 ON 11 GEX2 (661442..663289) Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway YLL067C 0 585 4301 ON 12 YLL067C complement(585..3915,4015..4301) Putative protein of unknown function with similarity to helicases YLL067W-A 1 843 1325 OFF 12 YLL067W-A (843..1325) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps YLL067C YLL066W-B 1 5605 5775 OFF 12 YLL066W-B (5605..5775) Putative protein of unknown function; overexpression causes a cell cycle delay or arrest YLL066C 0 6120 9836 ON 12 YLL066C complement(6120..9450,9550..9836) Putative protein of unknown function with similarity to helicases; YLL066C is not an essential gene YLL066W-A 1 6486 6968 OFF 12 YLL066W-A (6486..6968) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YLL066C YLL065W 1 11726 12076 OFF 12 YLL065W (11726..12076) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps chromosome XII telomeric element core sequences YLL064C 0 13083 13445 ON 12 PAU18 complement(13083..13445) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau6p YLL063C 0 14648 16072 ON 12 AYT1 complement(14648..16072) Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis YLL062C 0 16639 17613 ON 12 MHT1 complement(16639..17613) S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio YLL061W 1 17956 19707 ON 12 MMP1 (17956..19707) High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p YLL060C 0 21138 21839 ON 12 GTT2 complement(21138..21839) Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p YLL059C 0 22474 22980 OFF 12 YLL059C complement(22474..22980) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLL058W 1 23569 25296 ON 12 YLL058W (23569..25296) Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene YLL057C 0 25756 26994 ON 12 JLP1 complement(25756..26994) Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation YLL056C 0 27409 28305 ON 12 YLL056C complement(27409..28305) Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin YLL055W 1 30109 31704 ON 12 YCT1 (30109..31704) High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene YLL054C 0 32673 35204 ON 12 YLL054C complement(32673..35204) Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene YLL053C 0 35503 35961 OFF 12 YLL053C complement(35503..35961) Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin YLL052C 0 35912 36361 ON 12 AQY2 complement(35912..36361) Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains YLL051C 0 37333 39471 ON 12 FRE6 complement(37333..39471) Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels YLL050C 0 39804 40414 ON 12 COF1 complement(39804..40221,40401..40414) Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes YLL049W 1 40666 41205 ON 12 LDB18 (40666..41205) Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 YLL048C 0 41280 46265 ON 12 YBT1 complement(41280..46265) Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters YLL047W 1 46672 47055 OFF 12 YLL047W (46672..47055) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 YLL046C 0 46714 47463 ON 12 RNP1 complement(46714..47463) Ribonucleoprotein that contains two RNA recognition motifs (RRM) YLL045C 0 47859 48629 ON 12 RPL8B complement(47859..48629) Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits YLL044W 1 48216 48662 OFF 12 YLL044W (48216..48662) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant YLL043W 1 49938 51947 ON 12 FPS1 (49938..51947) Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress YLL042C 0 52087 52590 ON 12 ATG10 complement(52087..52590) Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy YLL041C 0 53131 53931 ON 12 SDH2 complement(53131..53931) Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain YLL040C 0 54211 63645 ON 12 VPS13 complement(54211..63645) Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively YLL039C 0 64062 65207 ON 12 UBI4 complement(64062..65207) Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats YLL038C 0 65775 66518 ON 12 ENT4 complement(65775..66518) Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome YLL037W 1 66562 66942 OFF 12 YLL037W (66562..66942) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor YLL036C 0 66745 68256 ON 12 PRP19 complement(66745..68256) Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain YLL035W 1 68580 70478 ON 12 GRC3 (68580..70478) Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated YLL034C 0 70633 73146 ON 12 RIX7 complement(70633..73146) Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions YLL033W 1 73409 74101 ON 12 IRC19 (73409..74101) Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci YLL032C 0 74270 76747 ON 12 YLL032C complement(74270..76747) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene YLL031C 0 77152 80205 ON 12 GPI13 complement(77152..80205) ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein YLL030C 0 80356 80697 OFF 12 RRT7 complement(80356..80697) Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLL029W 1 81461 83710 ON 12 FRA1 (81461..83710) Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation YLL028W 1 84804 86564 ON 12 TPO1 (84804..86564) Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane YLL027W 1 87403 88155 ON 12 ISA1 (87403..88155) Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins YLL026W 1 88623 91349 ON 12 HSP104 (88623..91349) Disaggregase; Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation Scer_12.trna1P 1 92548 92650 ON 12 tP-UGG (92548..92583,92615..92650) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 59.86 YLL025W 1 94747 95121 ON 12 PAU17 (94747..95121) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene YLL024C 0 95566 97485 ON 12 SSA2 complement(95566..97485) ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes YLL023C 0 97997 98836 ON 12 POM33 complement(97997..98836) Transmembrane nucleoporin involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p YLL022C 0 99044 100201 ON 12 HIF1 complement(99044..100201) Non-essential component of the HAT-B histone acetyltransferase complex (Hat1p-Hat2p-Hif1p), localized to the nucleus; has a role in telomeric silencing YLL021W 1 100947 105347 ON 12 SPA2 (100947..105347) Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate YLL020C 0 105556 105861 OFF 12 YLL020C complement(105556..105861) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 YLL019C 0 105686 107899 ON 12 KNS1 complement(105686..107899) Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation YLL019W-A 1 106908 107000 OFF 12 YLL019W-A (106908..107000) Dubious open reading frame unlikely to encode a protein; completely overlaps the verified gene KNS1 YLL018C-A 0 108676 108972 ON 12 COX19 complement(108676..108972) Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs YLL018C 0 109902 111575 ON 12 DPS1 complement(109902..111575) Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Scer_YGOB_SDC25 1 112234 115993 ON 12 SDC25 (112234..112506,112508..115993) ANNOTATED BY YGOB - skipping over frameshift in strain S288c YLL017W 1 112234 112545 OFF 12 YLL017W (112234..112545) Non-essential Ras guanine nucleotide exchange factor (GEF) localized to the membrane; expressed in poor nutrients and on non-fermentable carbon sources; homologous to CDC25; contains a stop codon in S288C; full-length gene includes YLL016W YLL016W 1 112847 115993 OFF 12 SDC25 (112847..115993) Non-essential Ras guanine nucleotide exchange factor (GEF) localized to the membrane; expressed in poor nutrients and on non-fermentable carbon sources; homologous to CDC25; contains a stop codon in S288C; full-length gene includes YLL017W YLL015W 1 116432 121111 ON 12 BPT1 (116432..121111) ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p YLL014W 1 121322 121648 ON 12 EMC6 (121322..121648) Member of a transmembrane complex required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response YLL013C 0 122075 124714 ON 12 PUF3 complement(122075..124714) Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins YLL012W 1 125534 127255 ON 12 YEH1 (125534..127255) Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes YLL011W 1 127523 128992 ON 12 SOF1 (127523..128992) Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p YLL010C 0 129330 130613 ON 12 PSR1 complement(129330..130613) Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p YLL009C 0 131205 131414 ON 12 COX17 complement(131205..131414) Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs YLL008W 1 131729 133987 ON 12 DRS1 (131729..133987) Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles YLL007C 0 134302 136299 ON 12 YLL007C complement(134302..136299) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene YLL006W-A 1 136345 136521 OFF 12 YLL006W-A (136345..136521) Putative protein of unknown function; identified by fungal homology and RT-PCR YLL006W 1 136586 137866 ON 12 MMM1 (136586..137866) ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth YLL005C 0 137940 140546 ON 12 SPO75 complement(137940..140546) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis YLL004W 1 141073 142923 ON 12 ORC3 (141073..142923) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing YLL003W 1 143201 146041 ON 12 SFI1 (143201..146041) Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C YLL002W 1 146291 147601 ON 12 RTT109 (146291..147601) Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p YLL001W 1 147890 150163 ON 12 DNM1 (147890..150163) Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Scer_CEN12 0 150828 150947 ON 12 CEN12 complement(150828..150947) Chromosome XII centromere YLR001C 0 151389 153977 ON 12 YLR001C complement(151389..153977) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated YLR002C 0 154343 156334 ON 12 NOC3 complement(154343..156334) Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation YLR003C 0 156855 157730 ON 12 CMS1 complement(156855..157730) Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable YLR004C 0 157934 159505 ON 12 THI73 complement(157934..159505) Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs YLR005W 1 160049 161434 ON 12 SSL1 (160049..161434) Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p YLR006C 0 161755 163893 ON 12 SSK1 complement(161755..163893) Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate YLR007W 1 164392 165402 ON 12 NSE1 (164392..165402) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair YLR008C 0 165577 166083 ON 12 PAM18 complement(165577..166083) Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p YLR009W 1 166537 167136 ON 12 RLP24 (166537..167136) Essential protein with similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S ribosomal subunits and required for ribosomal large subunit biogenesis YLR010C 0 167320 167802 ON 12 TEN1 complement(167320..167802) Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Scer_12.trna2S 0 167944 168025 ON 12 tS-AGA complement(167944..168025) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YLR011W 1 169103 169678 ON 12 LOT6 (169103..169678) FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions YLR012C 0 169982 170281 ON 12 YLR012C complement(169982..170281) Putative protein of unknown function; YLR012C is not an essential gene YLR013W 1 171339 171764 ON 12 GAT3 (171339..171764) Protein containing GATA family zinc finger motifs YLR014C 0 172268 174982 ON 12 PPR1 complement(172268..174982) Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p YLR015W 1 175227 176744 ON 12 BRE2 (175227..176744) Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L YLR016C 0 176802 177416 ON 12 PML1 complement(176802..177416) Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p YLR017W 1 177608 178621 ON 12 MEU1 (177608..178621) Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression YLR018C 0 178707 179606 ON 12 POM34 complement(178707..179606) Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication YLR019W 1 180288 181481 ON 12 PSR2 (180288..181481) Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p YLR020C 0 181789 183405 ON 12 YEH2 complement(181789..183405) Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism YLR021W 1 183623 184162 ON 12 IRC25 (183623..184162) Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions YLR022C 0 184174 184926 ON 12 SDO1 complement(184174..184926) Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes YLR023C 0 185498 187129 ON 12 IZH3 complement(185498..187129) Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity YLR024C 0 187663 193281 ON 12 UBR2 complement(187663..193281) Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex YLR025W 1 194452 195174 ON 12 SNF7 (194452..195174) One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes YLR026C 0 195450 196472 ON 12 SED5 complement(195450..196472) cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins YLR027C 0 196829 198085 ON 12 AAT2 complement(196829..198085) Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Scer_snR30 1 198784 199389 ON 12 SNR30 (198784..199389) H/ACA box small nucleolar RNA (snoRNA); essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA YLR028C 0 199540 201315 ON 12 ADE16 complement(199540..201315) Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine YLR029C 0 201976 202590 ON 12 RPL15A complement(201976..202590) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA YLR030W 1 203290 204081 OFF 12 YLR030W (203290..204081) Putative protein of unknown function YLR031W 1 204225 204785 ON 12 YLR031W (204225..204785) Putative protein of unknown function YLR032W 1 204991 208500 ON 12 RAD5 (204991..208500) DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p YLR033W 1 208761 210269 ON 12 RSC58 (208761..210269) Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance YLR034C 0 210512 211933 ON 12 SMF3 complement(210512..211933) Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins YLR035C 0 212369 214456 ON 12 MLH2 complement(212369..214456) Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Scer_12.trna3A 0 214883 214955 ON 12 tA-UGC complement(214883..214955) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: UGC | Cove Score: 76.57 YLR036C 0 221521 222132 ON 12 YLR036C complement(221521..222132) Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein YLR037C 0 222685 223059 ON 12 PAU23 complement(222685..223059) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth YLR038C 0 224921 225172 ON 12 COX12 complement(224921..225172) Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated YLR039C 0 225426 228596 ON 12 RIC1 complement(225426..228596) Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes YLR040C 0 228908 229582 ON 12 YLR040C complement(228908..229582) Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential YLR041W 1 229377 229697 OFF 12 YLR041W (229377..229697) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C YLR042C 0 229966 230451 ON 12 YLR042C complement(229966..230451) Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene YLR043C 0 231702 232013 ON 12 TRX1 complement(231702..232013) Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance YLR044C 0 232390 234081 ON 12 PDC1 complement(232390..234081) Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism YLR045C 0 235037 237703 ON 12 STU2 complement(235037..237703) Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p YLR046C 0 238001 238813 ON 12 YLR046C complement(238001..238813) Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance YLR047C 0 239347 241407 ON 12 FRE8 complement(239347..241407) Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p YLR048W 1 242232 243349 ON 12 RPS0B (242232..242321,242681..243349) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal YLR049C 0 243886 245172 ON 12 YLR049C complement(243886..245172) Putative protein of unknown function YLR050C 0 245587 246072 ON 12 YLR050C complement(245587..246072) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene YLR051C 0 246324 246977 ON 12 FCF2 complement(246324..246977) Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon YLR052W 1 247201 247953 ON 12 IES3 (247201..247953) Subunit of the INO80 chromatin remodeling complex YLR053C 0 248100 248426 ON 12 YLR053C complement(248100..248426) Putative protein of unknown function YLR054C 0 248717 250978 ON 12 OSW2 complement(248717..250860,250948..250978) Protein of unknown function proposed to be involved in the assembly of the spore wall YLR055C 0 251272 253080 ON 12 SPT8 complement(251272..253080) Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters YLR056W 1 253861 254958 ON 12 ERG3 (253861..254958) C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase YLR057W 1 255306 257855 ON 12 MNL2 (255306..257855) Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene YLR058C 0 257992 259401 ON 12 SHM2 complement(257992..259401) Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis YLR059C 0 259738 260547 ON 12 REX2 complement(259738..260547) 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease YLR060W 1 260979 262766 ON 12 FRS1 (260979..262766) Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control YLR061W 1 263194 263948 ON 12 RPL22A (263194..263205,263595..263948) Protein component of the large (60S) ribosomal subunit; exhibits similarity to Rpl22Bp and to rat L22 ribosomal protein; required for the oxidative stress response in yeast YLR062C 0 263577 263954 OFF 12 BUD28 complement(263577..263954) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay YLR063W 1 264157 265254 ON 12 YLR063W (264157..265254) Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene YLR064W 1 265456 266277 ON 12 PER33 (265456..266277) Protein that localizes to the endoplasmic reticulum with some nuclear pore complex association; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p YLR065C 0 266373 266918 ON 12 ENV10 complement(266373..266918) Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene YLR066W 1 267169 267723 ON 12 SPC3 (267169..267723) Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 YLR067C 0 267813 270710 ON 12 PET309 complement(267813..270710) Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) YLR068W 1 271008 271463 ON 12 FYV7 (271008..271463) Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin YLR069C 0 271630 273915 ON 12 MEF1 complement(271630..273915) Mitochondrial elongation factor involved in translational elongation YLR070C 0 274141 275211 ON 12 XYL2 complement(274141..275211) Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect YLR071C 0 275378 278626 ON 12 RGR1 complement(275378..278626) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation YLR072W 1 278862 280943 ON 12 YLR072W (278862..280943) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene YLR073C 0 281019 281621 ON 12 RFU1 complement(281019..281621) Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes YLR074C 0 281956 282456 ON 12 BUD20 complement(281956..282456) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YLR075W 1 282927 283592 ON 12 RPL10 (282927..283592) Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family YLR076C 0 283185 283607 OFF 12 YLR076C complement(283185..283607) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 YLR077W 1 283872 285623 ON 12 FMP25 (283872..285623) Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria YLR078C 0 285736 286559 ON 12 BOS1 complement(285736..286467,286557..286559) v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi YLR079W 1 286820 287674 ON 12 SIC1 (286820..287674) Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 YLR080W 1 287916 289250 ON 12 EMP46 (287916..289250) Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport YLR081W 1 290212 291936 ON 12 GAL2 (290212..291936) Galactose permease, required for utilization of galactose; also able to transport glucose YLR082C 0 292394 293572 ON 12 SRL2 complement(292394..293572) Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation YLR083C 0 294091 296094 ON 12 EMP70 complement(294091..296094) Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments YLR084C 0 296589 300251 ON 12 RAX2 complement(296589..300251) N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p YLR085C 0 300673 301989 ON 12 ARP6 complement(300673..301989) Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YLR086W 1 302243 306499 ON 12 SMC4 (302243..306499) Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate YLR087C 0 306855 315731 ON 12 CSF1 complement(306855..315731) Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR088W 1 316107 317951 ON 12 GAA1 (316107..317951) Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER YLR089C 0 318237 320015 ON 12 ALT1 complement(318237..320015) Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR090W 1 320701 322080 ON 12 XDJ1 (320701..322080) Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR091W 1 322297 323178 ON 12 GEP5 (322297..323178) Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine YLR092W 1 323544 326225 ON 12 SUL2 (323544..326225) High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates YLR093C 0 326513 327415 ON 12 NYV1 complement(326513..327258,327400..327415) v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane YLR094C 0 327730 329238 ON 12 GIS3 complement(327730..329238) Protein of unknown function YLR095C 0 329677 332115 ON 12 IOC2 complement(329677..332115) Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif YLR096W 1 332590 336033 ON 12 KIN2 (332590..336033) Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p YLR097C 0 336231 337265 ON 12 HRT3 complement(336231..337265) Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies YLR098C 0 337527 339473 ON 12 CHA4 complement(337527..339473) DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain YLR099C 0 339744 340928 ON 12 ICT1 complement(339744..340928) Lysophosphatidic acid acyltransferase, responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress YLR099W-A 1 341325 341588 ON 12 MIM2 (341325..341588) Mitochondrial protein required for outer membrane protein import; involved in import of the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and Fzo1p; component of a large protein complex in the outer membrane that includes Mim1p; not essential in W303 strain background YLR100W 1 341810 342853 ON 12 ERG27 (341810..342853) 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs YLR101C 0 342566 342961 OFF 12 YLR101C complement(342566..342961) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W YLR102C 0 342970 343767 ON 12 APC9 complement(342970..343767) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YLR103C 0 343989 345941 ON 12 CDC45 complement(343989..345941) DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes YLR104W 1 346585 346980 ON 12 LCL2 (346585..346980) Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) YLR105C 0 347047 348180 ON 12 SEN2 complement(347047..348180) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Scer_snR79 0 348427 348510 ON 12 SNR79 complement(348427..348510) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position C1007 YLR106C 0 349006 363738 ON 12 MDN1 complement(349006..363738) Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus YLR107W 1 364116 365330 ON 12 REX3 (364116..365330) RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases Scer_snR6 1 366235 366346 ON 12 SNR6 (366235..366346) U6 spliceosomal RNA (U6 snRNA), interacts with U4; homology to U6 snRNA, interacts with snr14 YLR108C 0 366667 368124 ON 12 YLR108C complement(366667..368124) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene YLR109W 1 368781 369311 ON 12 AHP1 (368781..369311) Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p YLR110C 0 369697 370098 ON 12 CCW12 complement(369697..370098) Cell wall mannoprotein with a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip YLR111W 1 370391 370723 OFF 12 YLR111W (370391..370723) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR112W 1 370791 371210 OFF 12 YLR112W (370791..371210) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR113W 1 371620 372927 ON 12 HOG1 (371620..372927) Mitogen-activated protein kinase involved in osmoregulation; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p Scer_12.trna4R 1 374355 374427 ON 12 tR-ACG (374355..374427) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 71.46 YLR114C 0 374944 377238 ON 12 AVL9 complement(374944..377238) Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms YLR115W 1 377985 380564 ON 12 CFT2 (377985..380564) Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. YLR116W 1 380822 382252 ON 12 MSL5 (380822..382252) Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA YLR117C 0 382471 384534 ON 12 CLF1 complement(382471..384534) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins YLR118C 0 384725 385408 ON 12 YLR118C complement(384725..385408) Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS YLR119W 1 385534 386175 ON 12 SRN2 (385534..386175) Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus YLR120C 0 386511 388220 ON 12 YPS1 complement(386511..388220) Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor YLR120W-A 1 388672 388773 OFF 12 YLR120W-A (388672..388773) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ORF YLR121C YLR121C 0 388744 390270 ON 12 YPS3 complement(388744..390270) Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor YLR122C 0 390954 391331 OFF 12 YLR122C complement(390954..391331) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C YLR123C 0 391078 391407 OFF 12 YLR123C complement(391078..391407) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif YLR124W 1 391600 391944 OFF 12 YLR124W (391600..391944) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR125W 1 393484 393894 ON 12 YLR125W (393484..393894) Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene YLR126C 0 394765 395520 ON 12 YLR126C complement(394765..395520) Putative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein YLR127C 0 395758 398319 ON 12 APC2 complement(395758..398319) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p YLR128W 1 398530 399433 ON 12 DCN1 (398530..398532,398627..399433) Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation YLR129W 1 399657 402488 ON 12 DIP2 (399657..402488) Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex YLR130C 0 402794 404062 ON 12 ZRT2 complement(402794..404062) Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor YLR131C 0 404510 406822 ON 12 ACE2 complement(404510..406822) Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit of Ace2p during the M-to-G1 transition, causing its specific localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of the RAM network that regulates cellular polarity and morphogenesis YLR132C 0 407283 408155 ON 12 USB1 complement(407283..408155) Essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants YLR133W 1 408445 410193 ON 12 CKI1 (408445..410193) Choline kinase, catalyzing the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway YLR134W 1 410723 412414 ON 12 PDC5 (410723..412414) Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism YLR135W 1 413281 415527 ON 12 SLX4 (413281..415527) Endonuclease involved in processing DNA during recombination and repair; cleaves branched structures in a complex with Slx1p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing YLR136C 0 415801 416658 ON 12 TIS11 complement(415801..416658) mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis YLR137W 1 417006 418109 ON 12 RKM5 (417006..418109) Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species YLR138W 1 418437 421394 ON 12 NHA1 (418437..421394) Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH YLR139C 0 421542 423473 ON 12 SLS1 complement(421542..423473) Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery YLR140W 1 423474 423800 OFF 12 YLR140W (423474..423800) Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I YLR141W 1 423683 424774 ON 12 RRN5 (423683..424774) Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p YLR142W 1 425186 426616 ON 12 PUT1 (425186..426616) Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Scer_12.trna5D 1 427132 427203 ON 12 tD-GUC (427132..427203) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YLR143W 1 427329 429386 ON 12 YLR143W (427329..429386) Putative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene YLR144C 0 429677 432016 ON 12 ACF2 complement(429677..432016) Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly YLR145W 1 432168 432773 ON 12 RMP1 (432168..432773) Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P YLR146C 0 432823 433725 ON 12 SPE4 complement(432823..433725) Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid YLR146W-A 1 433870 434058 ON 12 YLR146W-A (433870..434058) Putative protein of unknown function YLR147C 0 434158 434463 ON 12 SMD3 complement(434158..434463) Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 YLR148W 1 434641 437397 ON 12 PEP3 (434641..437397) Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis YLR149C 0 437631 439823 ON 12 YLR149C complement(437631..439823) Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene YLR149C-A 0 440370 440456 OFF 12 YLR149C-A complement(440370..440456) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR150W 1 440467 441288 ON 12 STM1 (440467..441288) Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure YLR151C 0 441715 442737 ON 12 PCD1 complement(441715..442737) Peroxisomal nudix pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids YLR152C 0 442958 444688 ON 12 YLR152C complement(442958..444688) Putative protein of unknown function; YLR152C is not an essential gene YLR153C 0 445524 447575 ON 12 ACS2 complement(445524..447575) Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions YLR154C 0 447982 448314 ON 12 RNH203 complement(447982..448314) Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Scer_12.trna6Q 0 448650 448721 ON 12 tQ-UUG complement(448650..448721) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Gln (Q) | Anti-Codon: UUG | Cove Score: 68.39 Scer_ETS2-1 0 451575 451785 OFF 12 ETS2-1 complement(451575..451785) Non-coding region located adjacent to and downstream of RDN25, transcribed as part of the 35S rRNA precursor transcript; contains the primary rRNA transcription termination site at +93 and a secondary termination site between +211 and +250 Scer_RDN37-1 0 451575 458432 OFF 12 RDN37-1 complement(451575..458432) 35S ribosomal RNA (35S rRNA) transcript; encoded by the RDN1 locus; processed into the 25S, 18S and 5.8S rRNAs (represented by the RDN25, RDN18, and RDN58 loci) Scer_RDN25-1 0 451786 455181 ON 12 RDN25-1 complement(451786..455181) 25S ribosomal RNA (25S rRNA), component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript YLR154W-A 1 452438 452623 OFF 12 YLR154W-A (452438..452623) Dubious open reading frame unlikely to encode a protein; encoded within the the 25S rRNA gene on the opposite strand YLR154W-B 1 454392 454556 OFF 12 YLR154W-B (454392..454556) Dubious open reading frame unlikely to encode a protein; encoded within the the 25S rRNA gene on the opposite strand YLR154W-C 1 454696 455070 OFF 12 TAR1 (454696..455070) Mitochondrial protein potentially involved in regulation of respiratory metabolism; interacts genetically with RPO41 and physically with Coq5p; encoded within the 25S rRNA gene on the opposite strand Scer_ITS2-1 0 455182 455413 OFF 12 ITS2-1 complement(455182..455413) Non-coding region between RDN58 and RDN25, that is transcribed as part of the 35S rRNA precursor transcript; forms a stem-loop structure that is required for processing of the precursor transcript Scer_RDN58-1 0 455414 455571 ON 12 RDN58-1 complement(455414..455571) 5.8S ribosomal RNA (5.8S rRNA); component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript; 3' end formation involves processing by the exosome complex while 5' end is generated by Kem1p and Rat1p YLR154W-E 1 455433 455636 OFF 12 YLR154W-E (455433..455636) Dubious open reading frame unlikely to encode a protein; encoded within the the 35S rRNA gene on the opposite strand Scer_ITS1-1 0 455572 455932 OFF 12 ITS1-1 complement(455572..455932) Non-coding region between RDN58 and RDN18, that is transcribed as part of the 35S rRNA precursor transcript; excision during transcript maturation is coupled with processing of the 3' external transcribed spacer (3'ETS) YLR154W-F 1 455883 456023 OFF 12 YLR154W-F (455883..456023) Dubious open reading frame unlikely to encode a protein; encoded within the the 35S rRNA gene on the opposite strand Scer_RDN18-1 0 455933 457732 ON 12 RDN18-1 complement(455933..457732) 18S ribosomal RNA (18S rRNA); component of the small (40S) ribosomal subunit; involved in codon recognition by tRNAs or the release factor eRF1 (Sup45p); encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript Scer_ETS1-1 0 457733 458432 OFF 12 ETS1-1 complement(457733..458432) Non-coding region located immediately upstream of RDN18, that is transcribed as part of the 35S rRNA precursor transcript; contains an essential U3 snoRNA binding site required for maturation of 18S rRNA Scer_NTS2-1 0 458433 459675 OFF 12 NTS2-1 complement(458433..459675) Nontranscribed region of the rDNA repeat, located between RND5 and the 5'ETS; contains RNA polymerase I termination site Scer_RDN5-1 1 459676 459796 ON 12 RDN5-1 (459676..459796) 5S ribosomal RNA (5S rRNA); only complete sequence of 6 repeated RDN5 alleles; able to support viability when provided as sole form of 5S rRNA; component of the large (60S) ribosomal subunit; localized to the nucleolus via interaction with Rpl5p; may play a role in translational frame fidelity; transcription is mediated by PolIII and activated by TFIIIA and TFIIIE Scer_NTS1-2 0 459797 460711 OFF 12 NTS1-2 complement(459797..460711) Non-transcribed region of the rDNA repeat located between the 3' ETS and RDN5, required for rDNA repeat expansion Scer_RDN37-2 0 460712 467569 OFF 12 RDN37-2 complement(460712..467569) 35S ribosomal RNA (35S rRNA) transcript; encoded by the RDN1 locus; processed into the 25S, 18S and 5.8S rRNAs (represented by the RDN25, RDN18, and RDN58 loci) Scer_ETS2-2 0 460712 460922 OFF 12 ETS2-2 complement(460712..460922) Non-coding region located adjacent to RDN25, transcribed as part of the 35S rRNA precursor transcript; contains the primary rRNA transcription termination site at +93 and a secondary termination site between +211 and +250 Scer_RDN25-2 0 460923 464318 OFF 12 RDN25-2 complement(460923..464318) 25S ribosomal RNA (25S rRNA), component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript YLR154C-G 0 462522 462671 OFF 12 YLR154C-G complement(462522..462671) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 Scer_ITS2-2 0 464319 464550 OFF 12 ITS2-2 complement(464319..464550) Non-coding region between RDN58 and RDN25, transcribed as part of the 35S rRNA precursor transcript; forms a stem-loop structure required for processing of the precursor transcript Scer_RDN58-2 0 464551 464708 OFF 12 RDN58-2 complement(464551..464708) 5.8S ribosomal RNA (5.8S rRNA); component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) Scer_ITS1-2 0 464709 465069 OFF 12 ITS1-2 complement(464709..465069) Non-coding region between RDN58 and RDN18, transcribed as part of the 35S rRNA precursor transcript; excision during transcript maturation is coupled with processing of the 3' external transcribed spacer (3' ETS) Scer_RDN18-2 0 465070 466869 OFF 12 RDN18-2 complement(465070..466869) 18S ribosomal RNA (18S rRNA); component of the small (40S) ribosomal subunit; involved in codon recognition by tRNAs or the release factor eRF1 (Sup45p); encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript Scer_ETS1-2 0 466870 467569 OFF 12 ETS1-2 complement(466870..467569) Non-coding region located immediately upstream of RDN18, transcribed as part of the 35S rRNA precursor transcript; contains an essential U3 snoRNA binding site required for maturation of 18S rRNA Scer_NTS2-2 0 467570 468812 OFF 12 NTS2-2 complement(467570..468812) Non-transcribed region of the rDNA repeat, located between RDN5 and the 5' ETS Scer_RDN5-2 1 468813 468931 OFF 12 RDN5-2 (468813..468931) Variant 5S ribosomal rRNA (5S rRNA) gene; located within the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-3, RDN5-4, and RDN5-5 YLR154C-H 0 468827 468958 OFF 12 YLR154C-H complement(468827..468958) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-2 YLR155C 0 469317 470405 ON 12 ASP3-1 complement(469317..470405) Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; reference strain S288C has four copies of ASP3 YLR156W 1 472113 472457 OFF 12 YLR156W (472113..472457) Putative protein of unknown function; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis Scer_RDN5-3 1 472465 472583 OFF 12 RDN5-3 (472465..472583) Variant 5S ribosomal RNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-4, and RDN5-5 YLR156C-A 0 472479 472610 OFF 12 YLR156C-A complement(472479..472610) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-3 YLR157C 0 472969 474057 ON 12 ASP3-2 complement(472969..474057) Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; reference strain S288C has four copies of ASP3 YLR157W-D 1 475764 475976 OFF 12 YLR157W-D (475764..475976) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YLR157W-E 1 481873 482037 OFF 12 YLR157W-E (481873..482037) Putative protein of unknown function identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element Scer_RDN5-4 1 482045 482163 OFF 12 RDN5-4 (482045..482163) Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-3, and RDN5-5 YLR157C-C 0 482059 482190 OFF 12 YLR157C-C complement(482059..482190) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-4 YLR158C 0 482549 483637 ON 12 ASP3-3 complement(482549..483637) Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; reference strain S288C has four copies of ASP3 YLR159W 1 485345 485689 OFF 12 YLR159W (485345..485689) Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 Scer_RDN5-5 1 485697 485815 OFF 12 RDN5-5 (485697..485815) Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-3, and RDN5-4 YLR159C-A 0 485711 485842 OFF 12 YLR159C-A complement(485711..485842) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-5 YLR160C 0 486201 487289 ON 12 ASP3-4 complement(486201..487289) Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; reference strain S288C has four copies of ASP3 YLR161W 1 488997 489341 OFF 12 YLR161W (488997..489341) Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 Scer_RDN5-6 1 489349 489469 ON 12 RDN5-6 (489349..489469) Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus YLR162W 1 489573 489929 OFF 12 YLR162W (489573..489929) Putative protein of unknown function; overexpression confers resistance to the antimicrobial peptide MiAMP1 and causes growth arrest, apoptosis, and increased sensitivity to cobalt chloride YLR162W-A 1 490406 490594 OFF 12 RRT15 (490406..490594) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; identified in a screen for mutants with decreased levels of rDNA transcription YLR163C 0 491866 493254 ON 12 MAS1 complement(491866..493254) Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins YLR163W-A 1 492812 492925 OFF 12 YLR163W-A (492812..492925) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YLR164W 1 493883 494389 ON 12 SHH4 (493883..494389) Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner YLR165C 0 494494 495258 ON 12 PUS5 complement(494494..495258) Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability YLR166C 0 495429 498044 ON 12 SEC10 complement(495429..498044) Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YLR167W 1 498947 499405 ON 12 RPS31 (498947..499405) Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B YLR168C 0 499578 500270 ON 12 UPS2 complement(499578..500270) Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI YLR169W 1 500335 500688 OFF 12 YLR169W (500335..500688) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR170C 0 500579 501049 ON 12 APS1 complement(500579..501049) Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex YLR171W 1 500733 501122 OFF 12 YLR171W (500733..501122) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR172C 0 501260 502162 ON 12 DPH5 complement(501260..502162) Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm YLR173W 1 502421 504247 ON 12 YLR173W (502421..504247) Putative protein of unknown function YLR174W 1 504591 505829 ON 12 IDP2 (504591..505829) Cytosolic NADP-specific isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose YLR175W 1 506134 507585 ON 12 CBF5 (506134..507585) Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita YLR176C 0 507797 510232 ON 12 RFX1 complement(507797..510232) Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins YLR177W 1 511054 512940 ON 12 YLR177W (511054..512940) Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; YLR177W is not an essential gene YLR178C 0 513162 513821 ON 12 TFS1 complement(513162..513821) Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase YLR179C 0 514108 514713 ON 12 YLR179C complement(514108..514713) Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus YLR180W 1 515262 516410 ON 12 SAM1 (515262..516410) S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) YLR181C 0 516678 517670 ON 12 VTA1 complement(516678..517670) Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain YLR182W 1 517940 520351 ON 12 SWI6 (517940..520351) Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators YLR183C 0 520543 522012 ON 12 TOS4 complement(520543..522012) Forkhead Associated domain containing protein and putative transcription factor found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; similar to PLM2 YLR184W 1 522106 522453 OFF 12 YLR184W (522106..522453) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR185W 1 522663 523288 ON 12 RPL37A (522663..522669,523029..523288) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Bp and to rat L37 ribosomal protein YLR186W 1 523632 524390 ON 12 EMG1 (523632..524390) Member of the alpha/beta knot fold methyltransferase superfamily; required for maturation of 18S rRNA and for 40S ribosome production; interacts with RNA and with S-adenosylmethionine; associates with spindle/microtubules; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome YLR187W 1 524865 527945 ON 12 SKG3 (524865..527945) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p YLR188W 1 528300 530387 ON 12 MDL1 (528300..530387) Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress YLR189C 0 530797 534393 ON 12 ATG26 complement(530797..534393) UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy YLR190W 1 535214 536689 ON 12 MMR1 (535214..536689) Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p YLR191W 1 537272 538432 ON 12 PEX13 (537272..538432) Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p YLR192C 0 538792 539589 ON 12 HCR1 complement(538792..539589) Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA YLR193C 0 540009 540536 ON 12 UPS1 complement(540009..540536) Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by promoting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has altered cardiolipin and phosphatidylethanolamine levels and defects in Mgm1p processing, integrity of mitochondrial inner membrane complexes, and mitochondrial morphology; ortholog of human PRELI YLR194C 0 540809 541573 ON 12 YLR194C complement(540809..541573) Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress YLR195C 0 541937 543304 ON 12 NMT1 complement(541937..543304) N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction YLR196W 1 543968 545698 ON 12 PWP1 (543968..545698) Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily YLR197W 1 546097 547611 ON 12 NOP56 (546097..547611) Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects YLR198C 0 547286 547645 OFF 12 YLR198C complement(547286..547645) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W YLR199C 0 547853 548769 ON 12 PBA1 complement(547853..548677,548764..548769) Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly YLR200W 1 549012 549356 ON 12 YKE2 (549012..549356) Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin YLR201C 0 549511 550293 ON 12 COQ9 complement(549511..550293) Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes YLR202C 0 550194 550636 OFF 12 YLR202C complement(550194..550458,550575..550636) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron YLR203C 0 550650 551960 ON 12 MSS51 complement(550650..551960) Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase YLR204W 1 552270 552605 ON 12 QRI5 (552270..552605) Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA YLR205C 0 552724 553677 ON 12 HMX1 complement(552724..553677) ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants YLR206W 1 554578 556419 ON 12 ENT2 (554578..556419) Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus YLR207W 1 556788 559289 ON 12 HRD3 (556788..559289) Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events YLR208W 1 559551 560444 ON 12 SEC13 (559551..560444) Structural component of three distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC), the COPII vesicle coat, and the Seh1-associated (SEA) complex; the COPII vesicle coat is required for vesicle formation in ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of genes or chromosomes at the nuclear periphery including transcription; homologous to human SEC13 YLR209C 0 560797 561732 ON 12 PNP1 complement(560797..561732) Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway YLR210W 1 562008 563390 ON 12 CLB4 (562008..563390) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation YLR211C 0 563792 564531 ON 12 YLR211C complement(563792..564454,564514..564531) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron YLR212C 0 564860 566281 ON 12 TUB4 complement(564860..566281) Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body YLR213C 0 566654 567922 ON 12 CRR1 complement(566654..567922) Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation YLR214W 1 568567 570627 ON 12 FRE1 (568567..570627) Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels YLR215C 0 570776 571858 ON 12 CDC123 complement(570776..571858) Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein YLR216C 0 572096 573211 ON 12 CPR6 complement(572096..573211) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity YLR217W 1 572909 573232 OFF 12 YLR217W (572909..573232) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 YLR218C 0 573466 573918 ON 12 COA4 complement(573466..573918) Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors YLR219W 1 574151 576337 ON 12 MSC3 (574151..576337) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate YLR220W 1 576825 577793 ON 12 CCC1 (576825..577793) Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation YLR221C 0 578362 579024 ON 12 RSA3 complement(578362..579024) Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus YLR222C 0 579318 581771 ON 12 UTP13 complement(579318..581771) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YLR222C-A 0 582006 582236 OFF 12 YLR222C-A complement(582006..582236) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene IFH1 YLR223C 0 582233 585490 ON 12 IFH1 complement(582233..585490) Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing YLR224W 1 586464 587573 ON 12 YLR224W (586464..587573) F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene YLR225C 0 587695 588918 ON 12 YLR225C complement(587695..588918) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene YLR226W 1 589354 590541 ON 12 BUR2 (589354..590541) Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II YLR227C 0 590562 592043 ON 12 ADY4 complement(590562..592043) Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Scer_12.trna7L 0 592519 592619 ON 12 tL-UAG complement(592519..592562,592582..592619) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAG | Cove Score: 60.39 YLR228C 0 600019 602463 ON 12 ECM22 complement(600019..602463) Sterol regulatory element binding protein, regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; homologous to Upc2p; relocates from intracellular membranes to perinuclear foci on sterol depletion YLR229C 0 604212 604787 ON 12 CDC42 complement(604212..604787) Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins YLR230W 1 604574 604879 OFF 12 YLR230W (604574..604879) Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Scer_12.trna8I 0 605300 605432 ON 12 tI-UAU complement(605300..605335,605396..605432) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: UAU | Cove Score: 61.58 YLR231C 0 605758 607119 ON 12 BNA5 complement(605758..607119) Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p YLR232W 1 606830 607177 OFF 12 YLR232W (606830..607177) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 YLR233C 0 607425 609524 ON 12 EST1 complement(607425..609524) TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA YLR234W 1 609783 611753 ON 12 TOP3 (609783..611753) DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination YLR235C 0 611532 611930 OFF 12 YLR235C complement(611532..611930) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TOP3 YLR236C 0 611794 612117 OFF 12 YLR236C complement(611794..612117) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR237W 1 612367 614163 ON 12 THI7 (612367..614163) Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia YLR238W 1 614755 616191 ON 12 FAR10 (614755..616191) Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate YLR239C 0 616332 617318 ON 12 LIP2 complement(616332..617318) Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups YLR240W 1 617533 620160 ON 12 VPS34 (617533..620160) Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p YLR241W 1 620473 622821 ON 12 YLR241W (620473..622821) Putative protein of unknown function, may be involved in detoxification YLR242C 0 622918 623883 ON 12 ARV1 complement(622918..623883) Protein functioning in transport of glycosylphosphatidylinositol intermediates into ER lumen; required for normal intracellular sterol distribution; human ARV1 required for normal cholesterol and bile acid homeostasis; similar to Nup120p YLR243W 1 624203 625021 ON 12 YLR243W (624203..625021) Putative protein of unknown function; YLR243W is an essential gene YLR244C 0 625168 626331 ON 12 MAP1 complement(625168..626331) Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p YLR245C 0 626502 626930 ON 12 CDD1 complement(626502..626930) Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm YLR246W 1 627118 628197 ON 12 ERF2 (627118..628197) Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Scer_12.trna9L 1 628383 628497 ON 12 tL-CAA (628383..628420,628454..628497) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 55.72 YLR247C 0 628684 633354 ON 12 IRC20 complement(628684..633354) Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci YLR248W 1 634252 636084 ON 12 RCK2 (634252..636084) Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases YLR249W 1 636780 639914 ON 12 YEF3 (636780..639914) Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP YLR250W 1 640317 641021 ON 12 SSP120 (640317..641021) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YLR251W 1 641464 642057 ON 12 SYM1 (641464..642057) Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 YLR252W 1 641957 642262 OFF 12 YLR252W (641957..642262) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism YLR253W 1 642627 644336 ON 12 YLR253W (642627..644336) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene YLR254C 0 644404 644973 ON 12 NDL1 complement(644404..644973) Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends YLR255C 0 645600 645953 OFF 12 YLR255C complement(645600..645953) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR256W 1 646415 650923 ON 12 HAP1 (646415..650923) Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus Scer_12.trna10A 0 656934 657006 ON 12 tA-AGC complement(656934..657006) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YLR257W 1 658826 659791 ON 12 YLR257W (658826..659791) Putative protein of unknown function YLR258W 1 660716 662833 ON 12 GSY2 (660716..662833) Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase YLR259C 0 663284 665002 ON 12 HSP60 complement(663284..665002) Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated YLR260W 1 665844 667907 ON 12 LCB5 (665844..667907) Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules YLR261C 0 668237 668563 OFF 12 VPS63 complement(668237..668563) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect YLR262C 0 668244 668891 ON 12 YPT6 complement(668244..668891) Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 YLR262C-A 0 669468 669662 ON 12 TMA7 complement(669468..669662) Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin YLR263W 1 670340 672823 ON 12 RED1 (670340..672823) Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p YLR264W 1 673131 673334 ON 12 RPS28B (673131..673334) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Ap and has similarity to rat S28 ribosomal protein YLR264C-A 0 673828 673944 OFF 12 YLR264C-A complement(673828..673944) Putative protein of unknown function YLR265C 0 674427 675455 ON 12 NEJ1 complement(674427..675455) Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p YLR266C 0 675619 677724 ON 12 PDR8 complement(675619..677724) Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon YLR267W 1 678212 679924 ON 12 BOP2 (678212..679924) Protein of unknown function YLR268W 1 680200 680844 ON 12 SEC22 (680200..680844) R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog YLR269C 0 680866 681216 OFF 12 YLR269C complement(680866..681216) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR270W 1 681186 682238 ON 12 DCS1 (681186..682238) Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium YLR271W 1 682737 683561 ON 12 YLR271W (682737..683561) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS YLR272C 0 683672 687202 ON 12 YCS4 complement(683672..687202) Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus Scer_12.trna11V 0 687859 687932 ON 12 tV-AAC complement(687859..687932) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YLR273C 0 689083 691029 ON 12 PIG1 complement(689083..691029) Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase YLR274W 1 691555 693882 ON 12 MCM5 (691555..693882) Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase YLR275W 1 694378 694800 ON 12 SMD2 (694378..694382,694473..694800) Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 YLR276C 0 695046 696830 ON 12 DBP9 complement(695046..696830) DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain YLR277C 0 697156 699495 ON 12 YSH1 complement(697156..699495) Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs YLR278C 0 699999 704024 ON 12 YLR278C complement(699999..704024) Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene YLR279W 1 704309 704698 OFF 12 YLR279W (704309..704698) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR280C 0 704483 704833 OFF 12 YLR280C complement(704483..704833) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR281C 0 704493 704960 ON 12 YLR281C complement(704493..704960) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene YLR282C 0 705071 705412 OFF 12 YLR282C complement(705071..705412) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition YLR283W 1 705186 706130 ON 12 YLR283W (705186..706130) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene YLR284C 0 706198 707040 ON 12 ECI1 complement(706198..707040) Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced YLR285W 1 707360 708145 ON 12 NNT1 (707360..708145) S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination YLR285C-A 0 708168 708338 OFF 12 YLR285C-A complement(708168..708338) Putative protein of unknown function; identified by fungal homology and RT-PCR YLR286C 0 708448 710136 ON 12 CTS1 complement(708448..710136) Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p YLR286W-A 1 708562 708696 OFF 12 YLR286W-A (708562..708696) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CTS1 YLR287C 0 710991 712058 ON 12 YLR287C complement(710991..712058) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene YLR287C-A 0 712537 713158 ON 12 RPS30A complement(712537..712725,713156..713158) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Bp and has similarity to rat S30 ribosomal protein YLR288C 0 713480 714904 ON 12 MEC3 complement(713480..714904) DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 YLR289W 1 715089 717026 ON 12 GUF1 (715089..717026) Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor YLR290C 0 717145 717978 ON 12 YLR290C complement(717145..717978) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene YLR291C 0 718317 719462 ON 12 GCD7 complement(718317..719462) Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YLR292C 0 719789 720370 ON 12 SEC72 complement(719789..720370) Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YLR293C 0 720771 721430 ON 12 GSP1 complement(720771..721430) Ran GTPase, GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; yeast Gsp2p homolog YLR294C 0 721701 722030 OFF 12 YLR294C complement(721701..722030) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 YLR295C 0 721999 722373 ON 12 ATP14 complement(721999..722373) Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YLR296W 1 722978 723304 OFF 12 YLR296W (722978..723304) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR297W 1 724044 724433 ON 12 YLR297W (724044..724433) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YLR297W is not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation YLR298C 0 724721 725416 ON 12 YHC1 complement(724721..725416) Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site YLR299W 1 726069 728051 ON 12 ECM38 (726069..728051) Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation YLR299C-A 0 727911 728003 OFF 12 YLR299C-A complement(727911..728003) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ECM38 YLR300W 1 728955 730301 ON 12 EXG1 (728955..730301) Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes YLR301W 1 730825 731559 ON 12 HRI1 (730825..731559) Protein of unknown function that interacts with Sec72p and Hrr25p Scer_12.trna12L 1 732090 732190 ON 12 tL-UAG (732090..732127,732147..732190) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAG | Cove Score: 61.03 YLR302C 0 732191 732553 OFF 12 YLR302C complement(732191..732553) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR303W 1 732542 733876 ON 12 MET17 (732542..733876) Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Scer_12.trna13I 1 734802 734875 ON 12 tI-AAU (734802..734875) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YLR304C 0 735212 737548 ON 12 ACO1 complement(735212..737548) Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy YLR305C 0 738161 743863 ON 12 STT4 complement(738161..743863) Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization YLR306W 1 744151 744851 ON 12 UBC12 (744151..744153,744288..744851) Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes YLR307W 1 745620 746525 ON 12 CDA1 (745620..746525) Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall YLR307C-A 0 746848 747111 ON 12 YLR307C-A complement(746848..747111) Putative protein of unknown function YLR308W 1 747937 748875 ON 12 CDA2 (747937..748875) Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall YLR309C 0 749034 751769 ON 12 IMH1 complement(749034..751769) Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi YLR310C 0 752224 756993 ON 12 CDC25 complement(752224..756993) Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 YLR311C 0 757265 757612 OFF 12 YLR311C complement(757265..757612) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR312C 0 757637 758833 ON 12 YLR312C complement(757637..758833) Putative protein of unknown function YLR312W-A 1 759480 760241 ON 12 MRPL15 (759480..760241) Mitochondrial ribosomal protein of the large subunit YLR313C 0 760750 762342 ON 12 SPH1 complement(760750..762342) Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p YLR314C 0 762575 764137 ON 12 CDC3 complement(762575..764137) Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells YLR315W 1 764808 765269 ON 12 NKP2 (764808..765269) Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) YLR316C 0 765266 766358 ON 12 TAD3 complement(765266..766073,766130..766181,766250..766358) Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs YLR317W 1 765655 766089 OFF 12 YLR317W (765655..766089) Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C YLR318W 1 766542 769196 ON 12 EST2 (766542..769196) Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia YLR319C 0 769318 771684 ON 12 BUD6 complement(769318..771684) Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate YLR320W 1 771940 776304 ON 12 MMS22 (771940..776304) Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation YLR321C 0 776584 777864 ON 12 SFH1 complement(776584..777864) Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog YLR322W 1 777628 777942 OFF 12 VPS65 (777628..777942) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth YLR323C 0 778173 778952 ON 12 CWC24 complement(778173..778952) Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p YLR324W 1 779215 780786 ON 12 PEX30 (779215..780786) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p YLR325C 0 781143 781379 ON 12 RPL38 complement(781143..781379) Protein component of the large (60S) ribosomal subunit, has similarity to rat L38 ribosomal protein YLR326W 1 782174 782896 ON 12 YLR326W (782174..782896) Putative protein of unknown function, predicted to be palmitoylated YLR327C 0 783127 783387 ON 12 TMA10 complement(783127..783387) Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p Scer_12.trna14X 1 784354 784453 ON 12 tX-??? (784354..784389,784410..784453) tRNA of undetermined specificity, predicted by tRNAscan-SE analysis; very similar to serine tRNAs; tRNA Type: Undet (X) | Anti-Codon: ??? | Cove Score: 57.54 YLR328W 1 784913 786118 ON 12 NMA1 (784913..786118) Nicotinic acid mononucleotide adenylyltransferase, involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways YLR329W 1 786442 787333 ON 12 REC102 (786442..786615,786713..787333) Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination YLR330W 1 787664 789679 ON 12 CHS5 (787664..789679) Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane YLR331C 0 790669 791046 OFF 12 JIP3 complement(790669..791046) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 YLR332W 1 790676 791806 ON 12 MID2 (790676..791806) O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Scer_12.trna15D 1 793918 793989 ON 12 tD-GUC (793918..793989) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 Scer_snR61 0 794486 794575 ON 12 SNR61 complement(794486..794575) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A1133 Scer_snR55 0 794697 794794 ON 12 SNR55 complement(794697..794794) C/D box small nucleolar RNA (snoRNA), predicted to guide 2'-O-methylation of small subunit (SSU) rRNA at position U1269 Scer_snR57 0 794937 795024 ON 12 SNR57 complement(794937..795024) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position G1572 YLR333C 0 795573 795899 ON 12 RPS25B complement(795573..795899) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein YLR334C 0 796698 797078 OFF 12 YLR334C complement(796698..797078) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Scer_12.trna16E 1 797178 797249 ON 12 tE-UUC (797178..797249) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YLR335W 1 797430 799592 ON 12 NUP2 (797430..799592) Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization YLR336C 0 799697 802396 ON 12 SGD1 complement(799697..802396) Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia YLR337C 0 802653 805106 ON 12 VRP1 complement(802653..805106) Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) YLR338W 1 804346 805203 OFF 12 OPI9 (804346..805203) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C YLR339C 0 805784 806335 OFF 12 YLR339C complement(805784..806335) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 YLR340W 1 805887 806825 ON 12 RPP0 (805887..806825) Conserved ribosomal protein P0 of the ribosomal stalk, which is involved in interaction between translational elongation factors and the ribosome; similar to rat P0, human P0, and E. coli L10e; phosphorylated on serine 302 YLR341W 1 807385 808818 ON 12 SPO77 (807385..808818) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis YLR342W 1 809997 815627 ON 12 FKS1 (809997..815627) Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling YLR342W-A 1 815810 815983 OFF 12 YLR342W-A (815810..815983) Putative protein of unknown function YLR343W 1 816094 817761 ON 12 GAS2 (816094..817761) 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Scer_12.trna17R 1 818609 818680 ON 12 tR-CCG (818609..818680) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; single essential nuclear tRNA gene containing the tDNA-anticodon CCG (presumably CCG in the mature tRNA), decodes CGG codons into arginine, one of 19 nuclear tRNAs for arginine; correct folding and efficient aminoacylation require Lhp1p; tRNA Type: Arg (R) | Anti-Codon: CCG | Cove Score: 57.65 YLR344W 1 819312 820142 ON 12 RPL26A (819312..819330,819778..820142) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Bp and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA YLR345W 1 820511 822040 ON 12 YLR345W (820511..822040) Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene YLR346C 0 822287 822592 ON 12 YLR346C complement(822287..822592) Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene YLR347C 0 823828 826413 ON 12 KAP95 complement(823828..826413) Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p YLR347W-A 1 824789 824929 OFF 12 YLR347W-A (824789..824929) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF KAP95/YLR347C YLR348C 0 826977 827873 ON 12 DIC1 complement(826977..827873) Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix YLR349W 1 827521 828027 OFF 12 YLR349W (827521..828027) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C YLR350W 1 828730 829380 ON 12 ORM2 (828730..829380) Evolutionarily conserved protein, similar to Orm1p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control YLR351C 0 829489 830364 ON 12 NIT3 complement(829489..830364) Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member YLR352W 1 831116 833539 ON 12 YLR352W (831116..833539) Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene YLR353W 1 834352 836163 ON 12 BUD8 (834352..836163) Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole YLR354C 0 836350 837357 ON 12 TAL1 complement(836350..837357) Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate YLR355C 0 838066 839253 ON 12 ILV5 complement(838066..839253) Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids YLR356W 1 840321 840914 ON 12 ATG33 (840321..840914) Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes YLR357W 1 841331 844000 ON 12 RSC2 (841331..844000) Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance YLR358C 0 843487 844050 OFF 12 YLR358C complement(843487..844050) Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W YLR359W 1 844282 845730 ON 12 ADE13 (844282..845730) Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency YLR360W 1 846103 847422 ON 12 VPS38 (846103..847422) Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity YLR361C 0 847388 849124 ON 12 DCR2 complement(847388..849124) Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START YLR361C-A 0 849383 849679 ON 12 YLR361C-A complement(849383..849679) Putative protein of unknown function YLR362W 1 849866 852019 ON 12 STE11 (849866..852019) Signal transducing MEK kinase involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p YLR363C 0 852495 853151 ON 12 NMD4 complement(852495..853151) Protein interacting with Nam7p, may be involved in the nonsense-mediated mRNA decay pathway YLR363W-A 1 853461 853718 ON 12 YLR363W-A (853461..853718) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YLR364W 1 854062 854391 ON 12 GRX8 (854062..854391) Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic YLR365W 1 855199 855531 OFF 12 YLR365W (855199..855531) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene YLR364C-A 0 855523 855645 OFF 12 YLR364C-A complement(855523..855645) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR366W YLR366W 1 855538 855843 OFF 12 YLR366W (855538..855843) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A YLR367W 1 856442 857317 ON 12 RPS22B (856442..856574,857058..857317) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Ap and has similarity to E. coli S8 and rat S15a ribosomal proteins Scer_snR44 1 856710 856920 OFF 12 SNR44 (856710..856920) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1056 and small subunit (SSU) rRNA at position U106 YLR368W 1 857540 859336 ON 12 MDM30 (857540..859336) F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains YLR369W 1 859552 861525 ON 12 SSQ1 (859552..861525) Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia YLR370C 0 861718 862254 ON 12 ARC18 complement(861718..862254) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YLR371W 1 862714 866784 ON 12 ROM2 (862714..866784) GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p YLR372W 1 867354 868391 ON 12 SUR4 (867354..868391) Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis YLR373C 0 868662 871367 ON 12 VID22 complement(868662..871367) Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles YLR374C 0 871453 871842 OFF 12 YLR374C complement(871453..871842) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W YLR375W 1 871697 872728 ON 12 STP3 (871697..872728) Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids YLR376C 0 872826 873554 ON 12 PSY3 complement(872826..873554) Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C YLR377C 0 873746 874792 ON 12 FBP1 complement(873746..874792) Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Scer_12.trna18K 1 875376 875471 ON 12 tK-UUU (875376..875412,875436..875471) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YLR378C 0 875736 877178 ON 12 SEC61 complement(875736..877178) Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER YLR379W 1 876922 877296 OFF 12 YLR379W (876922..877296) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C YLR380W 1 878282 879508 ON 12 CSR1 (878282..879508) Phosphatidylinositol transfer protein with a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance YLR381W 1 879723 881924 ON 12 CTF3 (879723..881924) Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules YLR382C 0 882067 884751 ON 12 NAM2 complement(882067..884751) Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance YLR383W 1 885288 888632 ON 12 SMC6 (885288..888632) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18 YLR384C 0 888851 892900 ON 12 IKI3 complement(888851..892900) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) YLR385C 0 892992 893390 ON 12 SWC7 complement(892992..893390) Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin YLR386W 1 893628 896270 ON 12 VAC14 (893628..896270) Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p YLR387C 0 896374 897672 ON 12 REH1 complement(896374..897672) Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains YLR388W 1 898651 898821 ON 12 RPS29A (898651..898821) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similarity to rat S29 and E. coli S14 ribosomal proteins Scer_snR34 1 899180 899382 ON 12 SNR34 (899180..899382) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2826 and U2880 YLR389C 0 899577 902660 ON 12 STE23 complement(899577..902660) Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family YLR390W 1 903066 903404 ON 12 ECM19 (903066..903404) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR390W-A 1 903724 904440 ON 12 CCW14 (903724..904440) Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall YLR392C 0 904749 906305 ON 12 ART10 complement(904749..906305) Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene YLR393W 1 907079 907918 ON 12 ATP10 (907079..907918) Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 YLR394W 1 907950 909398 ON 12 CST9 (907950..909398) SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate YLR395C 0 909729 909965 ON 12 COX8 complement(909729..909965) Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain YLR396C 0 910235 912310 ON 12 VPS33 complement(910235..912310) ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole YLR397C 0 912550 914892 ON 12 AFG2 complement(912550..914892) ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; may be involved in degradation of aberrant mRNAs YLR398C 0 915156 919019 ON 12 SKI2 complement(915156..919019) Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay YLR399C 0 919536 921596 ON 12 BDF1 complement(919536..921596) Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p YLR399W-A 1 920869 920973 OFF 12 YLR399W-A (920869..920973) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the characterized ORF BDF1/YLR399C YLR400W 1 922063 922536 OFF 12 YLR400W (922063..922536) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR401C 0 922442 924448 ON 12 DUS3 complement(922442..924448) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region YLR402W 1 924889 925080 OFF 12 YLR402W (924889..925080) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR403W 1 925568 927619 ON 12 SFP1 (925568..927619) Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion YLR404W 1 928743 929600 ON 12 FLD1 (928743..929600) Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy YLR405W 1 929789 930892 ON 12 DUS4 (929789..930892) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p YLR406C 0 931065 931755 ON 12 RPL31B complement(931065..931349,931699..931755) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality YLR406C-A 0 932206 932355 OFF 12 YLR406C-A complement(932206..932355) Putative protein of unknown function YLR407W 1 932967 933653 ON 12 YLR407W (932967..933653) Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus YLR408C 0 933885 934253 ON 12 BLS1 complement(933885..934253) Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene YLR409C 0 934414 937233 ON 12 UTP21 complement(934414..937233) Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma YLR410W 1 937541 940981 ON 12 VIP1 (937541..940981) Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 YLR411W 1 947253 947978 ON 12 CTR3 (947253..947978) High-affinity copper transporter of the plasma membrane, acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae YLR412W 1 948368 949192 ON 12 BER1 (948368..949192) Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene YLR412C-A 0 950267 950473 OFF 12 YLR412C-A complement(950267..950473) Putative protein of unknown function YLR413W 1 951156 953183 ON 12 YLR413W (951156..953183) Putative protein of unknown function; YLR413W is not an essential gene YLR414C 0 953353 954144 ON 12 PUN1 complement(953353..954144) Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress YLR415C 0 954259 954597 OFF 12 YLR415C complement(954259..954597) Putative protein of unknown function; YLR415C is not an essential gene YLR416C 0 954491 954889 OFF 12 YLR416C complement(954491..954889) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR417W 1 955010 956710 ON 12 VPS36 (955010..956710) Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome YLR418C 0 956914 958095 ON 12 CDC73 complement(956914..958095) Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay YLR419W 1 958428 962735 ON 12 YLR419W (958428..962735) Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Scer_12.trna19L 0 962972 963055 ON 12 tL-UAA complement(962972..963055) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YLR420W 1 963785 964879 ON 12 URA4 (963785..964879) Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate YLR421C 0 965090 965560 ON 12 RPN13 complement(965090..965560) Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability YLR422W 1 965897 971695 ON 12 YLR422W (965897..971695) Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein YLR423C 0 971917 973170 ON 12 ATG17 complement(971917..973170) Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells YLR424W 1 973395 975521 ON 12 SPP382 (973395..975521) Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Scer_12.trna20N 0 975983 976056 ON 12 tN-GUU complement(975983..976056) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YLR425W 1 982894 986817 ON 12 TUS1 (982894..986817) Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate YLR426W 1 987062 988113 ON 12 TDA5 (987062..987141,987213..988113) Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele YLR427W 1 988428 990440 ON 12 MAG2 (988428..990440) Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 YLR428C 0 990617 990961 OFF 12 YLR428C complement(990617..990961) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 YLR429W 1 990777 992732 ON 12 CRN1 (990777..992732) Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly YLR430W 1 993434 1000129 ON 12 SEN1 (993434..1000129) Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; homolog of Senataxin which causes Ataxia-Oculomotor Apraxia 2 and a dominant form of amyotrophic lateral sclerosis YLR431C 0 1000342 1001703 ON 12 ATG23 complement(1000342..1001703) Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p YLR432W 1 1002557 1004128 ON 12 IMD3 (1002557..1004128) Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed YLR433C 0 1004347 1006008 ON 12 CNA1 complement(1004347..1006008) Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 YLR434C 0 1006025 1006408 OFF 12 YLR434C complement(1006025..1006408) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W YLR435W 1 1006378 1006995 ON 12 TSR2 (1006378..1006995) Protein with a potential role in pre-rRNA processing YLR436C 0 1007421 1011245 ON 12 ECM30 complement(1007421..1011245) Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm YLR437C 0 1011622 1012023 ON 12 DIF1 complement(1011622..1012023) Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein YLR437C-A 0 1012459 1012686 OFF 12 YLR437C-A complement(1012459..1012686) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CAR2/YLR438W YLR438W 1 1012501 1013775 ON 12 CAR2 (1012501..1013775) L-ornithine transaminase (OTAse), catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive YLR438C-A 0 1013909 1014178 ON 12 LSM3 complement(1013909..1014178) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YLR439W 1 1014491 1015450 ON 12 MRPL4 (1014491..1015450) Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit YLR440C 0 1015568 1017697 ON 12 SEC39 complement(1015568..1017697) Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope YLR441C 0 1018141 1018908 ON 12 RPS1A complement(1018141..1018908) Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein YLR442C 0 1019315 1022251 ON 12 SIR3 complement(1019315..1022251) Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p YLR443W 1 1022625 1023971 ON 12 ECM7 (1022625..1023971) Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency YLR444C 0 1023686 1023988 OFF 12 YLR444C complement(1023686..1023988) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR445W 1 1024189 1024837 ON 12 GMC2 (1024189..1024572,1024655..1024837) Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor YLR446W 1 1025214 1026515 ON 12 YLR446W (1025214..1026515) Putative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene YLR447C 0 1026856 1027893 ON 12 VMA6 complement(1026856..1027893) Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membrane YLR448W 1 1028854 1029768 ON 12 RPL6B (1028854..1028868,1029253..1029768) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Ap and to rat L6 ribosomal protein; binds to 5.8S rRNA YLR449W 1 1030834 1032012 ON 12 FPR4 (1030834..1032012) Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones YLR450W 1 1032627 1035764 ON 12 HMG2 (1032627..1035764) One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks YLR451W 1 1036093 1038753 ON 12 LEU3 (1036093..1038753) Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation YLR452C 0 1039270 1041366 ON 12 SST2 complement(1039270..1041366) GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family YLR453C 0 1041799 1042986 ON 12 RIF2 complement(1041799..1042986) Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation YLR454W 1 1043998 1051884 ON 12 FMP27 (1043998..1051884) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Scer_12.trna21I 0 1052071 1052144 ON 12 tI-AAU complement(1052071..1052144) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YLR455W 1 1053629 1054543 ON 12 YLR455W (1053629..1054543) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YLR456W 1 1055070 1055684 ON 12 YLR456W (1055070..1055684) Putative protein of unknown function; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 YLR457C 0 1055812 1056771 ON 12 NBP1 complement(1055812..1056771) Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex YLR458W 1 1056455 1056835 OFF 12 YLR458W (1056455..1056835) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis YLR459W 1 1057334 1058518 ON 12 GAB1 (1057334..1058518) GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI YLR460C 0 1059757 1060887 ON 12 YLR460C complement(1059757..1060887) Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine YLR461W 1 1062919 1063281 ON 12 PAU4 (1062919..1063281) Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YLR462W 1 1065956 1066564 ON 12 YLR462W (1065956..1066564) Putative protein of unknown function with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII YLR463C 0 1066265 1066816 OFF 12 YLR463C complement(1066265..1066816) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORFs YLR462W and YLR464W YLR464W 1 1066572 1067501 ON 12 YLR464W (1066572..1067084,1067364..1067501) Putative protein of unknown function; intron is predicted but not detected experimentally; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C YLR465C 0 1067045 1067353 OFF 12 BSC3 complement(1067045..1067353) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 100% of YLR465C overlaps the uncharacterized ORF YLR464W and 86% of YLR465C overlaps the verified gene YRF1-4 YLR466W 1 1067087 1071235 ON 12 YRF1-4 (1067087..1071235) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YLR466C-A 0 1070387 1070869 OFF 12 YLR466C-A complement(1070387..1070869) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W YLR466C-B 0 1071594 1071710 OFF 12 YLR466C-B complement(1071594..1071710) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR467W 1 1072508 1077898 ON 12 YRF1-5 (1072508..1077898) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YLR467C-A 0 1077050 1077532 OFF 12 YLR467C-A complement(1077050..1077532) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W YML133C 0 461 4684 ON 13 YML133C complement(461..3791,3891..4684) Putative protein of unknown function with similarity to helicases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron YML133W-B 1 827 1309 OFF 13 YML133W-B (827..1309) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C YML133W-A 1 1610 2185 OFF 13 YML133W-A (1610..2185) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C YML132W 1 7244 8383 ON 13 COS3 (7244..8383) Protein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YML131W 1 10198 11295 ON 13 YML131W (10198..11295) Putative protein of unknown function with similarity to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm YML130C 0 11483 13174 ON 13 ERO1 complement(11483..13174) Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum YML129C 0 14541 14753 ON 13 COX14 complement(14541..14753) Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes YML128C 0 15135 16676 ON 13 MSC1 complement(15135..16676) Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated YML127W 1 17064 18809 ON 13 RSC9 (17064..18809) Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway YML126C 0 19060 20535 ON 13 ERG13 complement(19060..20535) 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis YML125C 0 20761 21699 ON 13 PGA3 complement(20761..21699) Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking YML124C 0 22048 23683 ON 13 TUB3 complement(22048..23360,23659..23683) Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p YML123C 0 24037 25800 ON 13 PHO84 complement(24037..25800) High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity YML122C 0 26038 26418 OFF 13 YML122C complement(26038..26418) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YML121W 1 26930 27862 ON 13 GTR1 (26930..27862) Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB YML120C 0 28266 29807 ON 13 NDI1 complement(28266..29807) NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; homolog of human AMID YML119W 1 30611 31684 ON 13 YML119W (30611..31684) Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate YML118W 1 32334 33851 ON 13 NGL3 (32334..33851) 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p YML117W 1 34243 37647 ON 13 NAB6 (34243..37647) Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP YML116W-A 1 37472 37774 OFF 13 YML116W-A (37472..37774) Putative protein of unknown function YML116W 1 38196 39824 ON 13 ATR1 (38196..39824) Multidrug efflux pump of the major facilitator superfamily, required for resistance to aminotriazole and 4-nitroquinoline-N-oxide YML115C 0 40187 41794 ON 13 VAN1 complement(40187..41794) Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant YML114C 0 42043 43575 ON 13 TAF8 complement(42043..43575) TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation YML113W 1 44045 44791 ON 13 DAT1 (44045..44791) DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability YML112W 1 45063 45953 ON 13 CTK3 (45063..45953) Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity YML111W 1 46942 49704 ON 13 BUL2 (46942..49704) Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1 YML110C 0 50031 50954 ON 13 COQ5 complement(50031..50954) 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes YML109W 1 51640 54468 ON 13 ZDS2 (51640..54468) Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; paralog of Zds1p YML108W 1 54793 55110 ON 13 YML108W (54793..55110) Putative protein of unknown function whose structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene YML107C 0 55265 56269 ON 13 PML39 complement(55265..56269) Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes YML106W 1 56773 57453 ON 13 URA5 (56773..57453) Major orotate phosphoribosyltransferase (OPRTase) isozyme that catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; minor OPRTase encoded by URA10 YML105C 0 57866 58687 ON 13 SEC65 complement(57866..58687) Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 YML104C 0 58939 62322 ON 13 MDM1 complement(58939..62322) Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) YML103C 0 62582 67549 ON 13 NUP188 complement(62582..67549) Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Scer_snR85 0 67768 67938 ON 13 SNR85 complement(67768..67938) H/ACA box small nucleolar RNA (snoRNA); predicted to guide pseudouridylation of small subunit (SSU) rRNA at position U1181 YML102W 1 68294 69700 ON 13 CAC2 (68294..69700) Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription YML101C-A 0 69409 69726 OFF 13 YML101C-A complement(69409..69726) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML101C 0 69735 70088 ON 13 CUE4 complement(69735..70088) Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination YML100W-A 1 70138 70311 OFF 13 YML100W-A (70138..70311) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YML100W 1 70624 73920 ON 13 TSL1 (70624..73920) Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, similar to Tps3p and may share function; mutant has aneuploidy tolerance YML099W-A 1 74229 74558 OFF 13 YML099W-A (74229..74558) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ARG81/YML099C YML099C 0 74398 77040 ON 13 ARG81 complement(74398..77040) Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p YML098W 1 77267 77770 ON 13 TAF13 (77267..77770) TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors YML097C 0 78335 79690 ON 13 VPS9 complement(78335..79690) A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin YML096W 1 79909 81486 ON 13 YML096W (79909..81486) Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 YML095C 0 81481 82113 ON 13 RAD10 complement(81481..82113) Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein YML094C-A 0 82219 82620 OFF 13 YML094C-A complement(82219..82620) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO YML094W 1 82275 82849 ON 13 GIM5 (82275..82290,82374..82849) Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it YML093W 1 83090 85789 ON 13 UTP14 (83090..85789) Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit YML092C 0 85987 86739 ON 13 PRE8 complement(85987..86739) Alpha 2 subunit of the 20S proteasome YML091C 0 87123 90731 ON 13 RPM2 complement(87123..90731) Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus YML090W 1 90744 91130 OFF 13 YML090W (90744..91130) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source YML089C 0 91041 91409 OFF 13 YML089C complement(91041..91409) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage YML088W 1 92235 94241 ON 13 UFO1 (92235..94241) F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation YML087C 0 94431 95369 ON 13 AIM33 complement(94431..95369) Putative protein of unknown function, highly conserved across species and orthologous to human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss YML086C 0 95791 97371 ON 13 ALO1 complement(95791..97371) D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress YML085C 0 97941 99400 ON 13 TUB1 complement(97941..99259,99376..99400) Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules YML084W 1 99489 99797 OFF 13 YML084W (99489..99797) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YML083C 0 99794 101050 ON 13 YML083C complement(99794..101050) Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions YML082W 1 101862 103811 ON 13 YML082W (101862..103811) Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene YML081C-A 0 103983 104162 ON 13 ATP18 complement(103983..104162) Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms YML081W 1 104777 108532 ON 13 TDA9 (104777..108532) DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene YML080W 1 108806 110077 ON 13 DUS1 (108806..110077) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 YML079W 1 110247 110852 ON 13 YML079W (110247..110852) Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm YML078W 1 111002 111550 ON 13 CPR3 (111002..111550) Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria YML077W 1 111865 112344 ON 13 BET5 (111865..112344) Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi YML076C 0 112513 115347 ON 13 WAR1 complement(112513..115347) Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively YML075C 0 115734 118898 ON 13 HMG1 complement(115734..118898) One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae YML074C 0 120089 121324 ON 13 FPR3 complement(120089..121324) Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p YML073C 0 123227 124172 ON 13 RPL6A complement(123227..123742,124158..124172) N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA YML072C 0 124730 129367 ON 13 TCB3 complement(124730..129367) Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact YML071C 0 129749 131572 ON 13 COG8 complement(129749..131572) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Scer_13.trna1R 0 131825 131896 ON 13 tR-UCU complement(131825..131896) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YML070W 1 133475 135229 ON 13 DAK1 (133475..135229) Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation YML069W 1 135500 137158 ON 13 POB3 (135500..137158) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases YML068W 1 137550 138944 ON 13 ITT1 (137550..138944) Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins YML067C 0 139063 140214 ON 13 ERV41 complement(139063..140090,140184..140214) Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein YML066C 0 140424 141533 ON 13 SMA2 complement(140424..141533) Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation YML065W 1 142210 144954 ON 13 ORC1 (142210..144954) Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity YML064C 0 145139 145876 ON 13 TEM1 complement(145139..145876) GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis YML063W 1 146482 147249 ON 13 RPS1B (146482..147249) Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein YML062C 0 147505 148683 ON 13 MFT1 complement(147505..148683) Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance YML061C 0 148953 151532 ON 13 PIF1 complement(148953..151532) DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis YML060W 1 151871 153001 ON 13 OGG1 (151871..153001) Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance YML059C 0 153219 158258 ON 13 NTE1 complement(153219..158258) Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes YML058W-A 1 158760 158966 ON 13 HUG1 (158760..158966) Protein involved in the Mec1p-mediated checkpoint pathway that responds to DNA damage or replication arrest, transcription is induced by DNA damage YML058W 1 159383 159697 ON 13 SML1 (159383..159697) Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase YML057C-A 0 160023 160412 OFF 13 YML057C-A complement(160023..160412) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W YML057W 1 160180 161994 ON 13 CMP2 (160180..161994) Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 YML056C 0 162194 164176 ON 13 IMD4 complement(162194..163308,163717..164176) Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Scer_snR54 0 163535 163620 OFF 13 SNR54 complement(163535..163620) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position A974 YML055W 1 164790 165326 ON 13 SPC2 (164790..165326) Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 YML054C 0 165533 167308 ON 13 CYB2 complement(165533..167308) Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions YML054C-A 0 167623 167781 OFF 13 YML054C-A complement(167623..167781) Putative protein of unknown function Scer_13.trna2Y 1 168795 168883 ON 13 tY-GUA (168795..168833,168848..168883) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.18 YML053C 0 169116 169754 ON 13 YML053C complement(169116..169754) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene YML052W 1 170402 171310 ON 13 SUR7 (170402..171310) Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants YML051W 1 171594 172901 ON 13 GAL80 (171594..172901) Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding YML050W 1 173139 174074 ON 13 AIM32 (173139..174074) Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss YML049C 0 174220 178305 ON 13 RSE1 complement(174220..178305) Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport YML048W 1 178426 179637 ON 13 GSF2 (178426..179637) ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression YML047W-A 1 179893 180258 OFF 13 YML047W-A (179893..180258) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML047C 0 180017 181075 ON 13 PRM6 complement(180017..181075) Pheromone-regulated protein, predicted to have 2 transmembrane segments; regulated by Ste12p during mating YML046W 1 181474 183363 ON 13 PRP39 (181474..183363) U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Scer_13.trna3G 0 183898 183968 ON 13 tG-GCC complement(183898..183968) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YML043C 0 190244 191767 ON 13 RRN11 complement(190244..191767) Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p YML042W 1 192788 194800 ON 13 CAT2 (192788..194800) Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes YML041C 0 194913 195755 ON 13 VPS71 complement(194913..195755) Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Scer_13.trna4P 0 196068 196170 ON 13 tP-UGG complement(196068..196103,196135..196170) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 60.49 YML038C 0 202775 204103 ON 13 YMD8 complement(202775..204103) Putative nucleotide sugar transporter, has similarity to Vrg4p YML037C 0 204386 205408 ON 13 YML037C complement(204386..205408) Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene YML036W 1 205642 206293 ON 13 CGI121 (205642..206097,206204..206293) Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification YML035C 0 206428 208860 ON 13 AMD1 complement(206428..208860) AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools YML034C-A 0 209428 209826 OFF 13 YML034C-A complement(209428..209826) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YML034W 1 209525 212155 ON 13 SRC1 (209525..211444,211571..212155) Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance YML032C 0 212515 213930 ON 13 RAD52 complement(212515..213930) Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis YML031C-A 0 214086 214421 OFF 13 YML031C-A complement(214086..214421) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF NDC1/YML031W; questionable ORF from MIPS YML031W 1 214189 216156 ON 13 NDC1 (214189..216156) Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 YML030W 1 216435 216914 ON 13 RCF1 (216435..216914) Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast YML029W 1 217362 219878 ON 13 USA1 (217362..219878) Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p YML028W 1 220138 220728 ON 13 TSA1 (220138..220728) Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype YML027W 1 221406 222563 ON 13 YOX1 (221406..222563) Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate YML026C 0 222987 223828 ON 13 RPS18B complement(222987..223380,223782..223828) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins YML025C 0 224406 225365 ON 13 YML6 complement(224406..225239,225339..225365) Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins YML024W 1 225889 226697 ON 13 RPS17A (225889..225891,226290..226697) Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein YML023C 0 226994 228664 ON 13 NSE5 complement(226994..228664) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair YML022W 1 228937 229500 ON 13 APT1 (228937..229500) Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis YML021C 0 229734 230813 ON 13 UNG1 complement(229734..230813) Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus YML020W 1 231149 233143 ON 13 YML020W (231149..233143) Putative protein of unknown function YML019W 1 233457 234455 ON 13 OST6 (233457..234455) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p YML018C 0 234771 235952 ON 13 YML018C complement(234771..235952) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene YML017W 1 236588 238731 ON 13 PSP2 (236588..236591,236954..238731) Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing YML016C 0 239458 241536 ON 13 PPZ1 complement(239458..241536) Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance YML015C 0 241989 243029 ON 13 TAF11 complement(241989..243029) TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors YML014W 1 243225 244064 ON 13 TRM9 (243225..244064) tRNA methyltransferase, catalyzes esterification of modified uridine nucleotides in tRNA(Arg3) and tRNA(Glu), likely as part of a complex with Trm112p; deletion confers resistance to zymocin YML013W 1 244149 245903 ON 13 UBX2 (244149..245903) Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis YML012C-A 0 245537 245914 OFF 13 YML012C-A complement(245537..245914) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 YML012W 1 246116 246751 ON 13 ERV25 (246116..246751) Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport YML011C 0 246895 247428 ON 13 RAD33 complement(246895..247428) Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YML010W 1 247677 250868 ON 13 SPT5 (247677..250868) Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair YML009W-B 1 250435 250911 OFF 13 YML009W-B (250435..250911) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition YML009C-A 0 250675 251001 OFF 13 YML009C-A complement(250675..251001) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML009C 0 251304 251516 ON 13 MRPL39 complement(251304..251516) Mitochondrial ribosomal protein of the large subunit YML008C 0 251839 252990 ON 13 ERG6 complement(251839..252990) Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane YML007C-A 0 253162 253272 OFF 13 YML007C-A complement(253162..253272) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria YML007W 1 253848 255800 ON 13 YAP1 (253848..255800) Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; mediates resistance to cadmium YML006C 0 256092 258416 ON 13 GIS4 complement(256092..258416) CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Scer_13.trna5S 0 259158 259239 ON 13 tS-AGA complement(259158..259239) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: AGA | Cove Score: 85.00 YML005W 1 260221 261609 ON 13 TRM12 (260221..261609) S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA YML004C 0 261705 262685 ON 13 GLO1 complement(261705..262685) Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress YML003W 1 263483 264355 OFF 13 YML003W (263483..264355) Putative protein of unknown function YML002W 1 264541 266754 ON 13 YML002W (264541..266754) Putative protein of unknown function; expression induced by heat and by calcium shortage YML001W 1 267174 267800 ON 13 YPT7 (267174..267800) Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, similar to mammalian Rab7 Scer_CEN13 1 268031 268149 ON 13 CEN13 (268031..268149) Chromosome XIII centromere YMR001C 0 269019 271136 ON 13 CDC5 complement(269019..271136) Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate YMR001C-A 0 271347 271577 OFF 13 YMR001C-A complement(271347..271577) Putative protein of unknown function YMR002W 1 272193 272663 ON 13 MIC17 (272193..272663) Mitochondrial intermembrane space protein, required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs YMR003W 1 273118 273714 ON 13 AIM34 (273118..273714) Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss YMR004W 1 274017 275552 ON 13 MVP1 (274017..275552) Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions YMR005W 1 276045 277211 ON 13 TAF4 (276045..277211) TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate YMR006C 0 277561 279681 ON 13 PLB2 complement(277561..279681) Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine YMR007W 1 279960 280340 OFF 13 YMR007W (279960..280340) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR008C 0 280590 282584 ON 13 PLB1 complement(280590..282584) Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol YMR009W 1 284102 284641 ON 13 ADI1 (284102..284641) Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions YMR010W 1 285100 286317 ON 13 YMR010W (285100..286317) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 YMR011W 1 288079 289704 ON 13 HXT2 (288079..289704) High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Scer_13.trna6E 1 290801 290872 ON 13 tE-UUC (290801..290872) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 57.99 YMR012W 1 291134 294967 ON 13 CLU1 (291134..294967) eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant YMR013C 0 295179 296738 ON 13 SEC59 complement(295179..296738) Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation YMR013C-A 0 296471 296620 OFF 13 YMR013C-A complement(296471..296620) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF SEC59/YML013C Scer_snR78 1 297278 297364 ON 13 SNR78 (297278..297364) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U2421 Scer_snR77 1 297506 297593 ON 13 SNR77 (297506..297593) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position U578 Scer_snR76 1 297725 297833 ON 13 SNR76 (297725..297833) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position C2197 Scer_snR75 1 297918 298006 ON 13 SNR75 (297918..298006) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position G2288 Scer_snR74 1 298138 298225 ON 13 SNR74 (298138..298225) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at position A28 Scer_snR73 1 298307 298412 ON 13 SNR73 (298307..298412) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position C2959 YMR013W-A 1 298311 298391 OFF 13 YMR013W-A (298311..298391) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Scer_snR72 1 298554 298651 ON 13 SNR72 (298554..298651) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A876 YMR014W 1 298868 300427 ON 13 BUD22 (298868..300427) Protein required for 18S rRNA maturation and small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern YMR015C 0 300869 302485 ON 13 ERG5 complement(300869..302485) C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs YMR016C 0 303236 305593 ON 13 SOK2 complement(303236..305593) Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors YMR017W 1 307489 308682 ON 13 SPO20 (307489..308682) Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog YMR018W 1 310208 311752 ON 13 YMR018W (310208..311752) Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene YMR019W 1 312156 315005 ON 13 STB4 (312156..315005) Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; YMR020W 1 315377 316903 ON 13 FMS1 (315377..316903) Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis YMR021C 0 317165 318418 ON 13 MAC1 complement(317165..318418) Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport YMR022W 1 318680 319177 ON 13 UBC7 (318680..319177) Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly YMR023C 0 319437 321017 ON 13 MSS1 complement(319437..321017) Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Scer_13.trna7A 0 321147 321219 ON 13 tA-AGC complement(321147..321219) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YMR024W 1 321875 323047 ON 13 MRPL3 (321875..323047) Mitochondrial ribosomal protein of the large subunit YMR025W 1 323300 324187 ON 13 CSI1 (323300..324187) Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling YMR026C 0 324236 325435 ON 13 PEX12 complement(324236..325435) C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder YMR027W 1 325877 327289 ON 13 YMR027W (325877..327289) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene YMR028W 1 327482 328582 ON 13 TAP42 (327482..328582) Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits YMR029C 0 328660 330231 ON 13 FAR8 complement(328660..330231) Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p YMR030W 1 330793 331923 ON 13 RSF1 (330793..331923) Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes YMR031C 0 332212 334743 ON 13 EIS1 complement(332212..334743) Component of the eisosome that is required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies YMR031W-A 1 334709 335035 OFF 13 YMR031W-A (334709..335035) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C YMR032W 1 335298 337307 ON 13 HOF1 (335298..337307) Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p YMR030W-A 1 337313 337603 OFF 13 YMR030W-A (337313..337603) Putative protein of unknown function YMR033W 1 337788 339277 ON 13 ARP9 (337788..337817,337904..339277) Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation YMR034C 0 339418 340722 ON 13 YMR034C complement(339418..340722) Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene YMR035W 1 341142 341675 ON 13 IMP2 (341142..341675) Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p YMR036C 0 341856 343520 ON 13 MIH1 complement(341856..343520) Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 YMR037C 0 344403 346517 ON 13 MSN2 complement(344403..346517) Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression YMR038C 0 347511 348260 ON 13 CCS1 complement(347511..348260) Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation YMR039C 0 348644 349522 ON 13 SUB1 complement(348644..349522) Transcriptional coactivator, facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes YMR040W 1 350381 350863 ON 13 YET2 (350381..350863) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein YMR041C 0 350966 351973 ON 13 ARA2 complement(350966..351973) NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase Scer_13.trna8F 1 352280 352370 ON 13 tF-GAA (352280..352316,352335..352370) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.38 YMR042W 1 352603 353136 ON 13 ARG80 (352603..353136) Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p YMR043W 1 353871 354731 ON 13 MCM1 (353871..354731) Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes YMR044W 1 355384 356811 ON 13 IOC4 (355384..356811) Member of a complex (Isw1b) with Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PWWP motif YMR046W-A 1 362702 362830 OFF 13 YMR046W-A (362702..362830) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_13.trna9H 1 363064 363135 ON 13 tH-GUG (363064..363135) Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis; tRNA Type: His (H) | Anti-Codon: GUG | Cove Score: 66.37 YMR047C 0 363364 366705 ON 13 NUP116 complement(363364..366705) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) YMR048W 1 366981 367934 ON 13 CSM3 (366981..367934) Replication fork associated factor, required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis YMR049C 0 368094 370517 ON 13 ERB1 complement(368094..370517) Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Scer_13.trna10V 0 372445 372518 ON 13 tV-AAC complement(372445..372518) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 Scer_13.trna11W 0 379303 379408 ON 13 tW-CCA complement(379303..379338,379373..379408) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YMR052W 1 379586 380200 ON 13 FAR3 (379586..380200) Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum YMR052C-A 0 380069 380434 OFF 13 YMR052C-A complement(380069..380434) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR053C 0 380346 382898 ON 13 STB2 complement(380346..382898) Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p YMR054W 1 383303 385975 ON 13 STV1 (383303..385975) Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole YMR055C 0 386101 387021 ON 13 BUB2 complement(386101..387021) Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage YMR056C 0 387315 388244 ON 13 AAC1 complement(387315..388244) Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator YMR057C 0 388359 388730 OFF 13 YMR057C complement(388359..388730) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 YMR058W 1 388822 390732 ON 13 FET3 (388822..390732) Ferro-O2-oxidoreductase required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases YMR059W 1 391099 391485 ON 13 SEN15 (391099..391485) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p YMR060C 0 391532 392515 ON 13 SAM37 complement(391532..392515) Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability YMR061W 1 392755 394788 ON 13 RNA14 (392755..394788) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping YMR062C 0 395054 396379 ON 13 ARG7 complement(395054..396379) Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine YMR063W 1 397077 397796 ON 13 RIM9 (397077..397796) Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalI; putative membrane protein YMR064W 1 397903 399459 ON 13 AEP1 (397903..399459) Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase YMR065W 1 399702 401216 ON 13 KAR5 (399702..401216) Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone YMR066W 1 401541 404237 ON 13 SOV1 (401541..404237) Mitochondrial protein of unknown function YMR067C 0 404323 405573 ON 13 UBX4 complement(404323..405573) UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome YMR068W 1 406304 407584 ON 13 AVO2 (406304..407584) Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth YMR069W 1 407709 408566 ON 13 NAT4 (407709..408566) N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A YMR070W 1 409154 410626 ON 13 MOT3 (409154..410626) Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion YMR071C 0 410762 411265 ON 13 TVP18 complement(410762..411265) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments YMR072W 1 411569 412120 ON 13 ABF2 (411569..412120) Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation YMR073C 0 412268 412873 ON 13 IRC21 complement(412268..412873) Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study YMR074C 0 413036 413473 ON 13 YMR074C complement(413036..413473) Protein with homology to human PDCD5, which is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene YMR075W 1 413982 416036 ON 13 RCO1 (413982..416036) Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p YMR075C-A 0 415688 416053 OFF 13 YMR075C-A complement(415688..416053) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W YMR076C 0 416196 420029 ON 13 PDS5 complement(416196..420029) Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Scer_13.trna12V 0 420588 420661 ON 13 tV-AAC complement(420588..420661) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YMR077C 0 421484 422149 ON 13 VPS20 complement(421484..422149) Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes YMR078C 0 422503 424728 ON 13 CTF18 complement(422503..424728) Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint YMR079W 1 424989 426059 ON 13 SEC14 (424989..424997,425154..426059) Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; functionally homologous to mammalian PITPs YMR080C 0 426712 429627 ON 13 NAM7 complement(426712..429627) ATP-dependent RNA helicase of the SFI superfamily involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance YMR081C 0 430079 431095 ON 13 ISF1 complement(430079..431095) Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant YMR082C 0 431769 432125 OFF 13 YMR082C complement(431769..432125) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR083W 1 434788 435915 ON 13 ADH3 (434788..435915) Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production YMR084W 1 436628 437416 OFF 13 YMR084W (436628..437416) Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains YMR085W 1 437491 438789 ON 13 YMR085W (437491..438789) Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains YMR086W 1 439208 442090 ON 13 SEG1 (439208..442090) Component of the eisosome with unknown function; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cell periphery; expression is repressed by cAMP; similar to A. gossypii SEG gene which is important for stabilizing eisosomes YMR086C-A 0 442026 442364 OFF 13 YMR086C-A complement(442026..442364) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR087W 1 442527 443381 ON 13 YMR087W (442527..443381) Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain YMR088C 0 443414 445102 ON 13 VBA1 complement(443414..445102) Permease of basic amino acids in the vacuolar membrane YMR089C 0 445609 448086 ON 13 YTA12 complement(445609..448086) Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes YMR090W 1 449245 449928 ON 13 YMR090W (449245..449928) Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability YMR091C 0 450058 451365 ON 13 NPL6 complement(450058..451365) Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions; involved in nuclear protein import and maintenance of proper telomere length YMR092C 0 451632 453479 ON 13 AIP1 complement(451632..453479) Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats YMR093W 1 454015 455556 ON 13 UTP15 (454015..455556) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YMR094W 1 455825 457261 ON 13 CTF13 (455825..457261) Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis YMR095C 0 457285 457959 ON 13 SNO1 complement(457285..457959) Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase YMR096W 1 458408 459301 ON 13 SNZ1 (458408..459301) Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins YMR097C 0 459424 460527 ON 13 MTG1 complement(459424..460527) Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals YMR098C 0 460771 462609 ON 13 ATP25 complement(460771..462609) Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Scer_13.trna13D 1 463554 463625 ON 13 tD-GUC (463554..463625) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YMR099C 0 463934 464827 ON 13 YMR099C complement(463934..464827) Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS YMR100W 1 466300 468162 ON 13 MUB1 (466300..468162) MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene YMR101C 0 468445 469476 ON 13 SRT1 complement(468445..469476) Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase YMR102C 0 469848 472352 ON 13 YMR102C complement(469848..472352) Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene YMR103C 0 472902 473264 OFF 13 YMR103C complement(472902..473264) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR104C 0 473420 475453 ON 13 YPK2 complement(473420..475453) Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK YMR105C 0 475897 477606 ON 13 PGM2 complement(475897..477606) Phosphoglucomutase, catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase YMR105W-A 1 478064 478258 OFF 13 YMR105W-A (478064..478258) Putative protein of unknown function YMR106C 0 478301 480190 ON 13 YKU80 complement(478301..480190) Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Scer_13.trna14K 0 480621 480693 ON 13 tK-CUU complement(480621..480693) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YMR107W 1 483014 483361 ON 13 SPG4 (483014..483361) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources YMR108W 1 484084 486147 ON 13 ILV2 (484084..486147) Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control YMR109W 1 486587 490246 ON 13 MYO5 (486587..490246) One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization YMR110C 0 490394 491992 ON 13 HFD1 complement(490394..491992) Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder YMR111C 0 492404 493792 ON 13 YMR111C complement(492404..493792) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene YMR112C 0 494100 494495 ON 13 MED11 complement(494100..494495) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein YMR113W 1 494999 496282 ON 13 FOL3 (494999..496282) Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis YMR114C 0 496343 497449 ON 13 YMR114C complement(496343..497449) Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene YMR115W 1 497704 499209 ON 13 MGR3 (497704..499209) Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA YMR116C 0 499456 500688 ON 13 ASC1 complement(499456..499878,500152..500688) G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; represses Gcn4p in the absence of amino acid starvation Scer_snR24 0 499984 500072 OFF 13 SNR24 complement(499984..500072) C/D box small nucleolar RNA (snoRNA); commonly referred to as U24; guides 2'-O-methylation of large subunit (LSU) rRNA at positions C1437, C1449, and C1450 YMR117C 0 501250 501891 ON 13 SPC24 complement(501250..501891) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering YMR118C 0 502144 502734 ON 13 SHH3 complement(502144..502734) Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Scer_13.trna15L 1 504895 505008 ON 13 tL-CAA (504895..504932,504965..505008) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YMR119W 1 505333 507207 ON 13 ASI1 (505333..507207) Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals YMR119W-A 1 506996 507370 OFF 13 YMR119W-A (506996..507370) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR120C 0 507502 509280 ON 13 ADE17 complement(507502..509280) Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine YMR121C 0 509734 510348 ON 13 RPL15B complement(509734..510348) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA YMR122C 0 510701 511075 OFF 13 YMR122C complement(510701..511075) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR122W-A 1 511315 511569 ON 13 YMR122W-A (511315..511569) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and endoplasmic reticulum YMR123W 1 513593 513961 ON 13 PKR1 (513593..513961) V-ATPase assembly factor, functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0) YMR124W 1 514456 517287 ON 13 YMR124W (514456..517287) Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization YMR125W 1 517539 520446 ON 13 STO1 (517539..517563,517886..520446) Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 YMR126C 0 520761 521789 ON 13 DLT1 complement(520761..521789) Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) YMR127C 0 522329 523345 ON 13 SAS2 complement(522329..523345) Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family YMR128W 1 523696 527499 ON 13 ECM16 (523696..527499) Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis YMR129W 1 527804 531817 ON 13 POM152 (527804..531817) Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 YMR130W 1 532119 533027 ON 13 YMR130W (532119..533027) Putative protein of unknown function; YMR130W is not an essential gene YMR131C 0 533163 534698 ON 13 RRB1 complement(533163..534698) Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p YMR132C 0 534944 535570 ON 13 JLP2 complement(534944..535570) Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) YMR133W 1 536207 537609 ON 13 REC114 (536207..537448,537565..537609) Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division YMR134W 1 537838 538551 ON 13 YMR134W (537838..538551) Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes YMR135C 0 538689 540056 ON 13 GID8 complement(538689..540056) Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains LisH and CTLH domains, like Vid30p; dosage-dependent regulator of START YMR135W-A 1 539911 540444 OFF 13 YMR135W-A (539911..540444) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR136W 1 541199 542881 ON 13 GAT2 (541199..542881) Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine YMR137C 0 542978 544963 ON 13 PSO2 complement(542978..544963) Nuclease required for a post-incision step in the repair of DNA single and double-strand breaks that result from interstrand crosslinks produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation YMR138W 1 545155 545730 ON 13 CIN4 (545155..545730) GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog YMR139W 1 546125 547237 ON 13 RIM11 (546125..547237) Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta YMR140W 1 547714 549183 ON 13 SIP5 (547714..549183) Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase YMR141C 0 549736 550044 OFF 13 YMR141C complement(549736..550044) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR142C 0 550206 551207 ON 13 RPL13B complement(550206..550801,551204..551207) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein YMR141W-A 1 550911 551135 OFF 13 YMR141W-A (550911..551135) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene RPL13B/YMR142C YMR143W 1 551928 552903 ON 13 RPS16A (551928..551951,552496..552903) Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins YMR144W 1 553362 554390 ON 13 YMR144W (553362..554390) Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene YMR145C 0 554793 556475 ON 13 NDE1 complement(554793..556475) Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain YMR146C 0 557481 558524 ON 13 TIF34 complement(557481..558524) eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation YMR147W 1 559199 559870 ON 13 YMR147W (559199..559870) Putative protein of unknown function YMR148W 1 560366 560812 ON 13 OSW5 (560366..560812) Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion YMR149W 1 560996 561856 ON 13 SWP1 (560996..561856) Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum YMR150C 0 561956 562528 ON 13 IMP1 complement(561956..562528) Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p YMR151W 1 562506 562943 OFF 13 YIM2 (562506..562943) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 YMR152W 1 563096 564193 ON 13 YIM1 (563096..564193) Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YMR153W 1 564435 565862 ON 13 NUP53 (564435..565862) FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis YMR153C-A 0 565574 565909 OFF 13 YMR153C-A complement(565574..565909) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 YMR154C 0 565999 568182 ON 13 RIM13 complement(565999..568182) Calpain-like cysteine protease involved in proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans palB YMR155W 1 568551 570194 ON 13 YMR155W (568551..570194) Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen YMR156C 0 570300 571016 ON 13 TPP1 complement(570300..571016) DNA 3'-phosphatase that functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; has similarity to the l-2-haloacid dehalogenase superfamily YMR157C 0 571277 572044 ON 13 AIM36 complement(571277..572044) Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies YMR158W 1 572248 572715 ON 13 MRPS8 (572248..572715) Mitochondrial ribosomal protein of the small subunit Scer_13.trna16M 0 572883 572955 ON 13 tM-CAU complement(572883..572955) Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 70.03 YMR158C-A 0 573194 573331 OFF 13 YMR158C-A complement(573194..573331) Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene YMR158W-B 1 574471 574791 OFF 13 YMR158W-B (574471..574791) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C YMR159C 0 574476 574928 ON 13 ATG16 complement(574476..574928) Conserved protein that interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy YMR160W 1 575066 577516 ON 13 YMR160W (575066..577516) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene YMR161W 1 577718 578392 ON 13 HLJ1 (577718..578392) Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ YMR162C 0 578951 583921 ON 13 DNF3 complement(578951..583921) Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase YMR163C 0 584271 586388 ON 13 INP2 complement(584271..586388) Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Scer_13.trna17V 0 586636 586709 ON 13 tV-AAC complement(586636..586709) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 68.42 YMR164C 0 587274 589550 ON 13 MSS11 complement(587274..589550) Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of MUC1 and STA2 in response to nutritional signals YMR165C 0 590040 592628 ON 13 PAH1 complement(590040..592628) Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol, responsible for de novo lipid synthesis and formation of lipid droplets; homologous to mammalian lipin 1 YMR166C 0 593367 594473 ON 13 YMR166C complement(593367..594473) Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene YMR167W 1 594886 597195 ON 13 MLH1 (594886..597195) Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer YMR168C 0 597332 599158 ON 13 CEP3 complement(597332..599158) Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain YMR169C 0 599352 600872 ON 13 ALD3 complement(599352..600872) Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose YMR170C 0 601562 603082 ON 13 ALD2 complement(601562..603082) Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p YMR171C 0 603868 605520 ON 13 EAR1 complement(603868..605520) Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p YMR172W 1 605981 608140 ON 13 HOT1 (605981..608140) Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p YMR172C-A 0 607828 608211 OFF 13 YMR172C-A complement(607828..608211) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR173W 1 608689 609981 ON 13 DDR48 (608689..609981) DNA damage-responsive protein, expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS YMR173W-A 1 608897 610081 OFF 13 YMR173W-A (608897..610081) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W YMR174C 0 610159 610365 ON 13 PAI3 complement(610159..610365) Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact YMR175W 1 611016 611255 ON 13 SIP18 (611016..611255) Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress YMR175W-A 1 611314 611508 OFF 13 YMR175W-A (611314..611508) Putative protein of unknown function YMR176W 1 611740 615975 ON 13 ECM5 (611740..615975) Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks YMR177W 1 616566 618098 ON 13 MMT1 (616566..618098) Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p YMR178W 1 618479 619303 ON 13 YMR178W (618479..619303) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR178W is not an essential gene YMR179W 1 619858 622134 ON 13 SPT21 (619858..622134) Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p YMR180C 0 622251 623213 ON 13 CTL1 complement(622251..623213) RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm YMR181C 0 623616 624080 ON 13 YMR181C complement(623616..624080) Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); YMR181C is not an essential gene YMR182C 0 624532 625167 ON 13 RGM1 complement(624532..625167) Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; deletion decreases expression of Y-prime telomeric elements; regulates expression of subtelomeric COS genes in conjunction with RNA binding protein Pub1p; localized to X elements within subtelomeric regions by ChIP YMR182W-A 1 625811 625897 OFF 13 YMR182W-A (625811..625897) Putative protein of unknown function Scer_snR83 1 626349 626654 ON 13 SNR83 (626349..626654) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at positions U1290 and U1415 YMR183C 0 626921 627808 ON 13 SSO2 complement(626921..627808) Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p YMR184W 1 628189 628785 ON 13 ADD37 (628189..628785) Protein of unknown function involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene YMR185W 1 629025 631970 ON 13 YMR185W (629025..631970) Putative protein of unknown function; conflicting evidence on whether null mutant is viable with elongated buds, or inviable YMR186W 1 632355 634472 ON 13 HSC82 (632355..634472) Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock YMR187C 0 634689 635984 ON 13 YMR187C complement(634689..635984) Putative protein of unknown function; YMR187C is not an essential gene YMR188C 0 636291 637004 ON 13 MRPS17 complement(636291..637004) Mitochondrial ribosomal protein of the small subunit YMR189W 1 637500 640604 ON 13 GCV2 (637500..640604) P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm YMR190C 0 640915 645258 ON 13 SGS1 complement(640915..645258) Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes YMR191W 1 645656 646777 ON 13 SPG5 (645656..646777) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources YMR192W 1 647118 649280 ON 13 GYL1 (647118..649280) Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth YMR193W 1 650036 650812 ON 13 MRPL24 (650036..650812) Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene YMR193C-A 0 651072 651458 OFF 13 YMR193C-A complement(651072..651458) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR194W 1 651145 651910 ON 13 RPL36A (651145..651160,651624..651910) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Bp and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA Scer_snR11 1 652275 652532 ON 13 SNR11 (652275..652532) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2416 YMR194C-B 0 652594 652887 ON 13 CMC4 complement(652594..652775,652848..652887) Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs YMR194C-A 0 652912 653136 OFF 13 YMR194C-A complement(652912..653136) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR195W 1 654034 654417 ON 13 ICY1 (654034..654417) Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation YMR196W 1 655076 658342 ON 13 YMR196W (655076..658342) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene YMR197C 0 658545 659198 ON 13 VTI1 complement(658545..659198) Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways YMR198W 1 659745 661529 ON 13 CIK1 (659745..661529) Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p YMR199W 1 662644 664284 ON 13 CLN1 (662644..664284) G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) YMR200W 1 664752 665522 ON 13 ROT1 (664752..665522) Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation YMR201C 0 665845 667044 ON 13 RAD14 complement(665845..666933,667018..667044) Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Scer_RNA170 0 667288 667456 ON 13 RNA170 complement(667288..667456) RNA polymerase III transcribed RNA of unknown function, conserved in other yeast species YMR202W 1 667537 668205 ON 13 ERG2 (667537..668205) C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis YMR203W 1 668492 669655 ON 13 TOM40 (668492..669655) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore YMR204C 0 670063 671325 ON 13 INP1 complement(670063..671325) Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane YMR205C 0 671887 674766 ON 13 PFK2 complement(671887..674766) Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes YMR206W 1 675896 676837 ON 13 YMR206W (675896..676837) Putative protein of unknown function; YMR206W is not an essential gene YMR207C 0 677193 683564 ON 13 HFA1 complement(677193..683564) Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis YMR208W 1 684467 685798 ON 13 ERG12 (684467..685798) Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate YMR209C 0 685911 687284 ON 13 YMR209C complement(685911..687284) Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene YMR210W 1 687516 688865 ON 13 YMR210W (687516..688865) Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification YMR211W 1 689083 690510 ON 13 DML1 (689083..690510) Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family YMR212C 0 690695 693043 ON 13 EFR3 complement(690695..693043) Protein required for Stt4-containing phosphoinositide kinase patch assembly at the plasma membrane; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene YMR213W 1 693381 695153 ON 13 CEF1 (693381..695153) Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p YMR214W 1 695350 696483 ON 13 SCJ1 (695350..696483) One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins YMR215W 1 696796 698370 ON 13 GAS3 (696796..698370) Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation YMR216C 0 698811 701039 ON 13 SKY1 complement(698811..701039) SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 YMR217W 1 701790 703367 ON 13 GUA1 (701790..703367) GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA YMR218C 0 703579 706887 ON 13 TRS130 complement(703579..706887) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1 YMR219W 1 707133 712109 ON 13 ESC1 (707133..712109) Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p YMR220W 1 712316 713671 ON 13 ERG8 (712316..713671) Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate YMR221C 0 713931 715445 ON 13 YMR221C complement(713931..715445) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy YMR222C 0 715638 716309 ON 13 FSH2 complement(715638..716309) Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 YMR223W 1 716715 718130 ON 13 UBP8 (716715..718130) Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B YMR224C 0 718575 720653 ON 13 MRE11 complement(718575..720653) Subunit of a complex with Rad50p and Xrs2p (MRX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for MRX function; widely conserved YMR225C 0 720960 721403 ON 13 MRPL44 complement(720960..721198,721346..721403) Mitochondrial ribosomal protein of the large subunit YMR226C 0 721593 722396 ON 13 YMR226C complement(721593..722396) NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments YMR227C 0 722613 724385 ON 13 TAF7 complement(722613..724385) TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation YMR228W 1 724626 725651 ON 13 MTF1 (724626..725651) Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation YMR229C 0 725934 731123 ON 13 RRP5 complement(725934..731123) RNA binding protein with preference for single stranded tracts of U's involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome YMR230W 1 732414 733141 ON 13 RPS10B (732414..732465,732876..733141) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Ap and has similarity to rat ribosomal protein S10 YMR230W-A 1 733268 733456 OFF 13 YMR230W-A (733268..733456) Putative protein of unknown function YMR231W 1 733545 736634 ON 13 PEP5 (733545..736634) Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p YMR232W 1 736926 738959 ON 13 FUS2 (736926..738959) Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating YMR233W 1 739185 739865 ON 13 TRI1 (739185..739865) Non-essential sumoylated protein of unknown function with similarity to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus YMR234W 1 740266 741312 ON 13 RNH1 (740266..741312) Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) YMR235C 0 741512 742735 ON 13 RNA1 complement(741512..742735) GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport YMR236W 1 742971 743444 ON 13 TAF9 (742971..743444) Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 YMR237W 1 743749 745923 ON 13 BCH1 (743749..745923) Member of the ChAPs family (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes YMR238W 1 746353 747729 ON 13 DFG5 (746353..747729) Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Scer_13.trna18R 0 747892 747963 ON 13 tR-UCU complement(747892..747963) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: UCU | Cove Score: 66.88 YMR239C 0 748262 749677 ON 13 RNT1 complement(748262..749677) RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination YMR240C 0 749930 751240 ON 13 CUS1 complement(749930..751240) Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p YMR241W 1 751961 752905 ON 13 YHM2 (751961..752905) Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome YMR242C 0 753225 754220 ON 13 RPL20A complement(753225..753742,754220..754220) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similarity to rat L18a ribosomal protein YMR242W-A 1 754297 754386 OFF 13 YMR242W-A (754297..754386) Putative protein of unknown function YMR243C 0 754838 756166 ON 13 ZRC1 complement(754838..756166) Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; also has a role in resistance to zinc shock resulting from a sudden influx of zinc into the cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain YMR244W 1 757250 758317 ON 13 YMR244W (757250..758317) Putative protein of unknown function YMR244C-A 0 758517 758831 ON 13 YMR244C-A complement(758517..758831) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS; YMR244C-A is not an essential gene; required for assembly of Cytochrome c Oxidase complex YMR245W 1 758563 759183 OFF 13 YMR245W (758563..759183) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR246W 1 759807 761891 ON 13 FAA4 (759807..761891) Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism Scer_snR86 0 762110 763113 ON 13 SNR86 complement(762110..763113) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2314 YMR247C 0 763351 768039 ON 13 RKR1 complement(763351..768039) RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Scer_13.trna19A 0 768369 768441 ON 13 tA-AGC complement(768369..768441) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YMR247W-A 1 769283 769426 OFF 13 YMR247W-A (769283..769426) Putative protein of unknown function YMR250W 1 770801 772558 ON 13 GAD1 (770801..772558) Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress YMR251W 1 772915 774015 ON 13 GTO3 (772915..774015) Omega class glutathione transferase; putative cytosolic localization YMR251W-A 1 774752 774931 ON 13 HOR7 (774752..774931) Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor YMR252C 0 775315 775719 ON 13 YMR252C complement(775315..775719) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene YMR253C 0 775946 777190 ON 13 YMR253C complement(775946..777190) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene YMR254C 0 777615 777923 OFF 13 YMR254C complement(777615..777923) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR255W 1 778001 778567 ON 13 GFD1 (778001..778567) Coiled-coiled protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation YMR256C 0 778945 779127 ON 13 COX7 complement(778945..779127) Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain YMR257C 0 779629 782031 ON 13 PET111 complement(779629..782031) Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane YMR258C 0 782620 784281 ON 13 ROY1 complement(782620..784281) GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p YMR259C 0 784621 788883 ON 13 YMR259C complement(784621..788883) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR259C is not an essential gene YMR260C 0 789378 789839 ON 13 TIF11 complement(789378..789839) Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 YMR261C 0 790205 793369 ON 13 TPS3 complement(790205..793369) Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway YMR262W 1 793726 794667 ON 13 YMR262W (793726..794667) Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene YMR263W 1 794919 795524 ON 13 SAP30 (794919..795524) Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance YMR264W 1 795805 796416 ON 13 CUE1 (795805..796416) Endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination YMR265C 0 796540 797925 ON 13 YMR265C complement(796540..797925) Putative protein of unknown function YMR266W 1 798518 801379 ON 13 RSN1 (798518..801379) Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane YMR267W 1 801772 802704 ON 13 PPA2 (801772..802704) Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate YMR268C 0 802888 804222 ON 13 PRP24 complement(802888..804222) Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling YMR269W 1 804456 805091 ON 13 TMA23 (804456..805091) Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan YMR270C 0 805326 806423 ON 13 RRN9 complement(805326..806423) Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I YMR271C 0 806865 807548 ON 13 URA10 complement(806865..807548) Minor orotate phosphoribosyltransferase (OPRTase) isozyme that catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; major OPRTase encoded by URA5 Scer_13.trna20Q 0 808246 808317 ON 13 tQ-CUG complement(808246..808317) Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; can mutate to cause constitutive pseudohyphal growth in homozygous diploids; tRNA Type: Gln (Q) | Anti-Codon: CUG | Cove Score: 64.54 YMR272C 0 809623 810777 ON 13 SCS7 complement(809623..810777) Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth YMR272W-A 1 810466 810579 OFF 13 YMR272W-A (810466..810579) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SCS7/YMR272C YMR272W-B 1 811089 811196 OFF 13 YMR272W-B (811089..811196) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YMR273C 0 811233 813980 ON 13 ZDS1 complement(811233..813980) Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at the telomere; has a role in Bcy1p localization; implicated in mRNA nuclear export YMR274C 0 814364 815311 ON 13 RCE1 complement(814364..815311) Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone YMR275C 0 815651 818581 ON 13 BUL1 complement(815651..818581) Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases YMR276W 1 818827 819948 ON 13 DSK2 (818827..819948) Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome YMR277W 1 820256 822454 ON 13 FCP1 (820256..822454) Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) YMR278W 1 822763 824631 ON 13 PGM3 (822763..824631) Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential YMR279C 0 824729 826351 ON 13 YMR279C complement(824729..826351) Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; paralog of the efflux pump ATR1 YMR280C 0 827028 831329 ON 13 CAT8 complement(827028..831329) Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements YMR281W 1 832339 833253 ON 13 GPI12 (832339..833253) ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp YMR282C 0 833356 835098 ON 13 AEP2 complement(833356..835098) Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader YMR283C 0 835326 836867 ON 13 RIT1 complement(835326..836867) 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Scer_13.trna21Y 1 837928 838016 ON 13 tY-GUA (837928..837966,837981..838016) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.18 YMR284W 1 838187 839995 ON 13 YKU70 (838187..839995) Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair YMR285C 0 840144 841691 ON 13 NGL2 complement(840144..841691) Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p YMR286W 1 841942 842202 ON 13 MRPL33 (841942..842202) Mitochondrial ribosomal protein of the large subunit YMR287C 0 842436 845345 ON 13 DSS1 complement(842436..845345) 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs YMR288W 1 845571 848486 ON 13 HSH155 (845571..848486) U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 YMR289W 1 848685 849809 ON 13 ABZ2 (848685..849809) Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis YMR290C 0 850074 851591 ON 13 HAS1 complement(850074..851591) ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles YMR290W-A 1 851422 851769 OFF 13 YMR290W-A (851422..851769) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase YMR291W 1 852630 854390 ON 13 TDA1 (852630..854390) Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene YMR292W 1 854795 855293 ON 13 GOT1 (854795..854816,854899..855293) Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition YMR293C 0 855399 856793 ON 13 HER2 complement(855399..856793) Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase YMR294W 1 856966 858087 ON 13 JNM1 (856966..858087) Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B YMR294W-A 1 858209 858568 OFF 13 YMR294W-A (858209..858568) Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents YMR295C 0 858297 858890 ON 13 YMR295C complement(858297..858890) Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene YMR296C 0 859215 860891 ON 13 LCB1 complement(859215..860891) Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine YMR297W 1 861922 863520 ON 13 PRC1 (861922..863520) Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family YMR298W 1 863819 864271 ON 13 LIP1 (863819..864271) Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis YMR299C 0 864412 865350 ON 13 DYN3 complement(864412..865350) Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration YMR300C 0 865559 867091 ON 13 ADE4 complement(865559..867091) Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway YMR301C 0 867555 869627 ON 13 ATM1 complement(867555..869627) Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol YMR302C 0 870073 872625 ON 13 YME2 complement(870073..872625) Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases YMR303C 0 873291 874337 ON 13 ADH2 complement(873291..874337) Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 YMR304W 1 874987 878679 ON 13 UBP15 (874987..878679) Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p YMR304C-A 0 878780 879130 OFF 13 YMR304C-A complement(878780..879130) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 YMR305C 0 879063 880232 ON 13 SCW10 complement(879063..880232) Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p YMR306W 1 881159 886516 ON 13 FKS3 (881159..886516) Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YMR306C-A 0 886183 886572 OFF 13 YMR306C-A complement(886183..886572) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR307W 1 887003 888682 ON 13 GAS1 (887003..888682) Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery YMR307C-A 0 887731 887925 OFF 13 YMR307C-A complement(887731..887925) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GAS1/YMR307W YMR308C 0 888952 892221 ON 13 PSE1 complement(888952..892221) Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p YMR309C 0 892988 895426 ON 13 NIP1 complement(892988..895426) eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection YMR310C 0 895716 896669 ON 13 YMR310C complement(895716..896669) Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene YMR311C 0 896914 897603 ON 13 GLC8 complement(896914..897603) Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2 YMR312W 1 898405 899226 ON 13 ELP6 (898405..899226) Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity YMR313C 0 899379 901307 ON 13 TGL3 complement(899379..901307) Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation YMR314W 1 901709 902413 ON 13 PRE5 (901709..902413) Alpha 6 subunit of the 20S proteasome YMR315W 1 902800 903849 ON 13 YMR315W (902800..903849) Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site YMR315W-A 1 904286 904393 OFF 13 YMR315W-A (904286..904393) Putative protein of unknown function YMR316W 1 904825 905835 ON 13 DIA1 (904825..905835) Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YMR316C-A 0 905662 905973 OFF 13 YMR316C-A complement(905662..905973) Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W YMR316C-B 0 907321 907629 OFF 13 YMR316C-B complement(907321..907629) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YMR317W 1 907364 910786 ON 13 YMR317W (907364..910786) Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene YMR318C 0 911061 912143 ON 13 ADH6 complement(911061..912143) NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance YMR319C 0 912880 914538 ON 13 FET4 complement(912880..914538) Low-affinity Fe(II) transporter of the plasma membrane YMR320W 1 916746 917051 OFF 13 YMR320W (916746..917051) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR321C 0 917579 917896 ON 13 YMR321C complement(917579..917896) Putative protein of unknown function; proposed to be a palmitoylated membrane protein YMR322C 0 918366 919079 ON 13 SNO4 complement(918366..919079) Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation YMR323W 1 920088 921401 ON 13 ERR3 (920088..921401) Protein of unknown function, has similarity to enolases YMR324C 0 922202 922444 OFF 13 YMR324C complement(922202..922444) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; transcription is AZF1 dependent in glycerol-lactate medium and SLT2 dependent in response to the lipid hydroperoxides YMR325W 1 922641 923015 ON 13 PAU19 (922641..923015) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions YMR326C 0 923494 923802 OFF 13 YMR326C complement(923494..923802) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 YNL339C 0 371 6098 ON 14 YRF1-6 complement(371..5931,6080..6098) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YNL339W-B 1 734 1216 OFF 14 YNL339W-B (734..1216) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YNL339W-A 1 1517 2092 OFF 14 YNL339W-A (1517..2092) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YNL338W 1 6561 6719 OFF 14 YNL338W (6561..6719) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV YNL337W 1 7165 7419 OFF 14 YNL337W (7165..7419) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL336W 1 8330 9475 ON 14 COS1 (8330..9475) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YNL335W 1 11452 12129 ON 14 DDI3 (11452..12129) Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants YNL334C 0 12208 12876 ON 14 SNO2 complement(12208..12876) Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin YNL333W 1 13267 14163 ON 14 SNZ2 (13267..14163) Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p YNL332W 1 14832 15854 ON 14 THI12 (14832..15854) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YNL331C 0 16118 17248 ON 14 AAD14 complement(16118..17248) Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YNL330C 0 18001 19302 ON 14 RPD3 complement(18001..19302) Histone deacetylase; regulates transcription, silencing, and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members YNL329C 0 19541 22633 ON 14 PEX6 complement(19541..22633) AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol YNL328C 0 22834 23274 ON 14 MDJ2 complement(22834..23274) Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain YNL327W 1 24048 27173 ON 14 EGT2 (24048..27173) Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner YNL326C 0 27337 28347 ON 14 PFA3 complement(27337..28347) Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions YNL325C 0 28739 31378 ON 14 FIG4 complement(28739..31378) Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain YNL324W 1 31050 31445 OFF 14 YNL324W (31050..31445) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YNL323W 1 31944 33188 ON 14 LEM3 (31944..33188) Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane YNL322C 0 33294 34235 ON 14 KRE1 complement(33294..34235) Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor YNL321W 1 34696 37422 ON 14 VNX1 (34696..37422) Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate YNL320W 1 37700 38554 ON 14 YNL320W (37700..38554) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL319W 1 38642 39082 OFF 14 YNL319W (38642..39082) Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 YNL318C 0 38707 40329 ON 14 HXT14 complement(38707..40329) Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL317W 1 40619 42016 ON 14 PFS2 (40619..42016) Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex YNL316C 0 42071 43075 ON 14 PHA2 complement(42071..43075) Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway YNL315C 0 43324 44280 ON 14 ATP11 complement(43324..44280) Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase YNL314W 1 44447 45214 ON 14 DAL82 (44447..45214) Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain YNL313C 0 45308 48022 ON 14 EMW1 complement(45308..48022) Essential conserved protein with a role in maintaining cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1 YNL312W 1 48287 49216 ON 14 RFA2 (48287..48293,48402..49216) Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination YNL311C 0 49397 51688 ON 14 SKP2 complement(49397..51688) F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments YNL310C 0 51907 52431 ON 14 ZIM17 complement(51907..52431) Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix YNL309W 1 52662 53924 ON 14 STB1 (52662..53924) Protein with a role in regulation of MBF-specific transcription at Start, phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p and binding is required for Stb1p function; expression is cell-cycle regulated YNL308C 0 54122 55897 ON 14 KRI1 complement(54122..55897) Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p YNL307C 0 56446 57573 ON 14 MCK1 complement(56446..57573) Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry into meiosis; related to mammalian glycogen synthase kinases of the GSK-3 family YNL306W 1 58155 58808 ON 14 MRPS18 (58155..58808) Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins YNL305C 0 58898 59791 ON 14 BXI1 complement(58898..59791) Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p YNL304W 1 60297 61550 ON 14 YPT11 (60297..61550) Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells YNL303W 1 61510 61857 OFF 14 YNL303W (61510..61857) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL302C 0 61958 62943 ON 14 RPS19B complement(61958..62372,62924..62943) Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Ap YNL301C 0 63570 64562 ON 14 RPL18B complement(63570..64018,64451..64562) Protein component of the large (60S) ribosomal subunit, identical to Rpl18Ap and has similarity to rat L18 ribosomal protein YNL300W 1 65744 66052 ON 14 TOS6 (65744..66052) Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid YNL299W 1 66517 68445 ON 14 TRF5 (66517..68445) Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p YNL298W 1 68915 71443 ON 14 CLA4 (68915..71443) Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p YNL297C 0 71673 76583 ON 14 MON2 complement(71673..76583) Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins YNL296W 1 76273 76587 OFF 14 YNL296W (76273..76587) Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog YNL295W 1 76946 78520 ON 14 YNL295W (76946..78520) Putative protein of unknown function YNL294C 0 78659 80260 ON 14 RIM21 complement(78659..80260) Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH YNL293W 1 80640 82541 ON 14 MSB3 (80640..82541) GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth YNL292W 1 82806 84017 ON 14 PUS4 (82806..84017) Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) YNL291C 0 84169 85815 ON 14 MID1 complement(84169..85815) N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer YNL290W 1 86218 87240 ON 14 RFC3 (86218..87240) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YNL289W 1 87897 88736 ON 14 PCL1 (87897..88736) Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth Scer_snR40 1 89210 89306 ON 14 SNR40 (89210..89306) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U898 and small subunit (SSU) rRNA at position G1271 YNL288W 1 90303 91424 ON 14 CAF40 (90303..91424) Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p YNL287W 1 91994 94801 ON 14 SEC21 (91994..94801) Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo YNL286W 1 95223 96080 ON 14 CUS2 (95223..96080) Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) YNL285W 1 96173 96544 OFF 14 YNL285W (96173..96544) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_14.trna1G 0 96241 96312 ON 14 tG-UCC complement(96241..96312) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: UCC | Cove Score: 66.72 Scer_14.trna2N 1 102716 102789 ON 14 tN-GUU (102716..102789) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YNL284C 0 103134 104102 ON 14 MRPL10 complement(103134..104102) Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Scer_14.trna3T 1 104805 104877 ON 14 tT-AGU (104805..104877) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YNL283C 0 105184 106695 ON 14 WSC2 complement(105184..106695) Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response YNL282W 1 107687 108274 ON 14 POP3 (107687..108274) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs YNL281W 1 108467 108928 ON 14 HCH1 (108467..108928) Heat shock protein regulator that binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus YNL280C 0 109095 110411 ON 14 ERG24 complement(109095..110411) C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions YNL279W 1 110917 112902 ON 14 PRM1 (110917..112902) Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p YNL278W 1 113271 116453 ON 14 CAF120 (113271..116453) Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation YNL277W-A 1 116679 116867 OFF 14 YNL277W-A (116679..116867) Putative protein of unknown function YNL277W 1 117349 118809 ON 14 MET2 (117349..118809) L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway YNL276C 0 118431 118826 OFF 14 YNL276C complement(118431..118826) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W YNL275W 1 119268 120998 ON 14 BOR1 (119268..120998) Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 YNL274C 0 121118 122170 ON 14 GOR1 complement(121118..122170) Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL273W 1 122883 126599 ON 14 TOF1 (122883..126599) Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase YNL272C 0 126805 129084 ON 14 SEC2 complement(126805..129084) Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles YNL271C 0 129522 135383 ON 14 BNI1 complement(129522..135383) Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 YNL270C 0 135940 137661 ON 14 ALP1 complement(135940..137661) Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression YNL269W 1 137699 138094 OFF 14 BSC4 (137699..138094) Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p YNL268W 1 138550 140385 ON 14 LYP1 (138550..140385) Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids YNL267W 1 140878 144078 ON 14 PIK1 (140878..144078) Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton YNL266W 1 144245 144664 OFF 14 YNL266W (144245..144664) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C YNL265C 0 144280 145281 ON 14 IST1 complement(144280..145149,145255..145281) Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p YNL264C 0 145563 146615 ON 14 PDR17 complement(145563..146615) Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition YNL263C 0 146896 147840 ON 14 YIF1 complement(146896..147840) Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 YNL262W 1 148212 154880 ON 14 POL2 (148212..154880) Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p YNL261W 1 155100 156539 ON 14 ORC5 (155100..156539) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing YNL260C 0 156859 157455 ON 14 YNL260C complement(156859..157455) Putative protein of unknown function with similarity to a human protein overexpressed in oral cancers; essential gene with defects in anaerobic and filamentous growth; localizes to the nucleus and cytoplasm YNL259C 0 157644 157865 ON 14 ATX1 complement(157644..157865) Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake YNL258C 0 158109 160373 ON 14 DSL1 complement(158109..160373) Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene YNL257C 0 160631 164320 ON 14 SIP3 complement(160631..164320) Protein that activates transcription through interaction with DNA-bound Snf1p, C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate YNL256W 1 164623 167097 ON 14 FOL1 (164623..167097) Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities YNL255C 0 167329 167790 ON 14 GIS2 complement(167329..167790) Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 YNL254C 0 168043 169248 ON 14 RTC4 complement(168043..169248) Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YNL253W 1 170018 171286 ON 14 TEX1 (170018..171286) Protein involved in mRNA export, component of the transcription export (TREX) complex YNL252C 0 171441 172286 ON 14 MRPL17 complement(171441..172286) Mitochondrial ribosomal protein of the large subunit YNL251C 0 172588 174315 ON 14 NRD1 complement(172588..174315) RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; YNL250W 1 175410 179348 ON 14 RAD50 (175410..179348) Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining YNL249C 0 179394 181022 ON 14 MPA43 complement(179394..181022) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL248C 0 181361 182608 ON 14 RPA49 complement(181361..182608) RNA polymerase I subunit A49 YNL247W 1 182875 185178 ON 14 YNL247W (182875..185178) Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments YNL246W 1 185460 186349 ON 14 VPS75 (185460..185491,185587..186349) NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair YNL245C 0 186346 186885 ON 14 CWC25 complement(186346..186885) Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p YNL244C 0 187170 187496 ON 14 SUI1 complement(187170..187496) Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase YNL243W 1 188051 190957 ON 14 SLA2 (188051..190957) Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo YNL242W 1 191324 196102 ON 14 ATG2 (191324..196102) Peripheral membrane protein required for vesicle formation during autophagy, pexophagy, and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria YNL241C 0 196426 197943 ON 14 ZWF1 complement(196426..197943) Glucose-6-phosphate dehydrogenase (G6PD), catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia YNL240C 0 198502 199977 ON 14 NAR1 complement(198502..199977) Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf YNL239W 1 200569 201933 ON 14 LAP3 (200569..201933) Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH YNL238W 1 202428 204872 ON 14 KEX2 (202428..204872) Subtilisin-like protease (proprotein convertase), a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway YNL237W 1 205188 206567 ON 14 YTP1 (205188..206567) Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins YNL236W 1 206930 209854 ON 14 SIN4 (206930..209854) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription YNL235C 0 209548 209979 OFF 14 YNL235C complement(209548..209979) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex YNL234W 1 210233 211513 ON 14 YNL234W (210233..211513) Protein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1 YNL233W 1 211922 214600 ON 14 BNI4 (211922..214600) Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p YNL232W 1 214923 215801 ON 14 CSL4 (214923..215801) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) YNL231C 0 215987 217042 ON 14 PDR16 complement(215987..217042) Phosphatidylinositol transfer protein (PITP) controlled by the multiple drug resistance regulator Pdr1p, localizes to lipid particles and microsomes, controls levels of various lipids, may regulate lipid synthesis, homologous to Pdr17p YNL230C 0 217523 218662 ON 14 ELA1 complement(217523..218662) Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex YNL229C 0 219137 220201 ON 14 URE2 complement(219137..220201) Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion YNL228W 1 220645 221421 OFF 14 YNL228W (220645..221421) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 YNL227C 0 220659 222431 ON 14 JJJ1 complement(220659..222431) Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis YNL226W 1 222239 222649 OFF 14 YNL226W (222239..222649) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C YNL225C 0 222724 224469 ON 14 CNM67 complement(222724..224469) Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration YNL224C 0 224796 227099 ON 14 SQS1 complement(224796..227099) Stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles YNL223W 1 227370 228854 ON 14 ATG4 (227370..228854) Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation YNL222W 1 229094 229714 ON 14 SSU72 (229094..229714) Transcription/RNA-processing factor that associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 of the RNA polymerase II C-terminal domain; affects start site selection in vivo Scer_snR19 0 230105 230672 ON 14 SNR19 complement(230105..230672) U1 spliceosomal RNA (U1 snRNA); snRNA U1 homolog YNL221C 0 231068 233695 ON 14 POP1 complement(231068..233695) Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P YNL220W 1 234413 235714 ON 14 ADE12 (234413..235714) Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence YNL219C 0 235996 237663 ON 14 ALG9 complement(235996..237663) Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation YNL218W 1 238238 240001 ON 14 MGS1 (238238..240001) Protein with DNA-dependent ATPase and ssDNA annealing activities involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP) YNL217W 1 240331 241311 ON 14 YNL217W (240331..241311) Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate YNL216W 1 241689 244172 ON 14 RAP1 (241689..244172) Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) YNL215W 1 244468 245430 ON 14 IES2 (244468..245430) Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; essential for growth under anaerobic conditions YNL214W 1 245617 246216 ON 14 PEX17 (245617..246216) Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis YNL213C 0 246460 247104 ON 14 RRG9 complement(246460..247104) Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL212W 1 247461 249809 ON 14 VID27 (247461..249809) Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth YNL211C 0 250055 250315 ON 14 YNL211C complement(250055..250315) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene YNL210W 1 250931 251743 ON 14 MER1 (250931..251743) Protein with RNA-binding motifs required for meiosis-specific mRNA splicing; required for chromosome pairing and meiotic recombination; Mer1p regulon embraces four essential meiotic pre-mRNAs: REC107, HFM1, AMA1 and SPO22 YNL209W 1 252059 253900 ON 14 SSB2 (252059..253900) Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; homolog of SSB1 YNL208W 1 254418 255017 ON 14 YNL208W (254418..255017) Protein of unknown function; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi YNL207W 1 255353 256630 ON 14 RIO2 (255353..256630) Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p YNL206C 0 256788 258155 ON 14 RTT106 complement(256788..258155) Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition YNL205C 0 258155 258577 OFF 14 YNL205C complement(258155..258577) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YNL204C 0 258375 259277 ON 14 SPS18 complement(258375..259277) Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation YNL203C 0 259439 260050 OFF 14 YNL203C complement(259439..260050) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YNL202W 1 259578 260456 ON 14 SPS19 (259578..260456) Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate YNL201C 0 260627 263203 ON 14 PSY2 complement(260627..263203) Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 YNL200C 0 263713 264453 ON 14 YNL200C complement(263713..264453) NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL199C 0 264926 266530 ON 14 GCR2 complement(264926..266530) Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p YNL198C 0 266514 266816 OFF 14 YNL198C complement(266514..266816) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL197C 0 267608 269593 ON 14 WHI3 complement(267608..269593) RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start YNL196C 0 270275 271171 ON 14 SLZ1 complement(270275..271171) Sporulation-specific protein with a leucine zipper motif YNL195C 0 271523 272308 ON 14 YNL195C complement(271523..272308) Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL194C 0 272710 273615 ON 14 YNL194C complement(272710..273615) Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YNL193W 1 274368 276044 ON 14 YNL193W (274368..276044) Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis YNL192W 1 276502 279897 ON 14 CHS1 (276502..279897) Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor YNL191W 1 280432 281505 ON 14 DUG3 (280432..281505) Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) YNL190W 1 282395 283009 ON 14 YNL190W (282395..283009) Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site YNL189W 1 284260 285888 ON 14 SRP1 (284260..285888) Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation YNL188W 1 286308 287609 ON 14 KAR1 (286308..287609) Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p YNL187W 1 287994 289067 ON 14 SWT21 (287994..289067) Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats YNL186W 1 289498 291876 ON 14 UBP10 (289498..291876) Ubiquitin-specific protease that deubiquitinates ubiquitin-protein moieties; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p and possibly other transporters; primarily located in the nucleus YNL185C 0 292193 292669 ON 14 MRPL19 complement(292193..292669) Mitochondrial ribosomal protein of the large subunit YNL184C 0 292557 292883 OFF 14 YNL184C complement(292557..292883) Protein of unknown function; expressed at both mRNA and protein levels YNL183C 0 293137 295509 ON 14 NPR1 complement(293137..295509) Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex YNL182C 0 295960 297627 ON 14 IPI3 complement(295960..297627) Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; highly conserved and contains WD40 motifs; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles YNL181W 1 298335 299558 ON 14 YNL181W (298335..299558) Putative oxidoreductase; required for cell viability YNL180C 0 299654 300649 ON 14 RHO5 complement(299654..300649) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity YNL179C 0 300666 301103 OFF 14 YNL179C complement(300666..301103) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance YNL178W 1 302680 303402 ON 14 RPS3 (302680..303402) Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; has similarity to E. coli S3 and rat S3 ribosomal proteins YNL177C 0 303686 304615 ON 14 MRPL22 complement(303686..304615) Mitochondrial ribosomal protein of the large subunit YNL176C 0 305070 306980 ON 14 TDA7 complement(305070..306980) Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele YNL175C 0 307401 308612 ON 14 NOP13 complement(307401..308612) Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM) YNL174W 1 308074 308646 OFF 14 YNL174W (308074..308646) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene NOP13/YNL175C YNL173C 0 308957 310057 ON 14 MDG1 complement(308957..310057) Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations YNL172W 1 310636 315882 ON 14 APC1 (310636..315882) Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis YNL171C 0 315629 315997 OFF 14 YNL171C complement(315629..315997) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YNL170W 1 315978 316373 OFF 14 YNL170W (315978..316373) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YNL169C 0 316169 317671 ON 14 PSD1 complement(316169..317671) Phosphatidylserine decarboxylase of the mitochondrial inner membrane, converts phosphatidylserine to phosphatidylethanolamine YNL168C 0 318030 318809 ON 14 FMP41 complement(318030..318809) Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL167C 0 319416 321359 ON 14 SKO1 complement(319416..321359) Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses YNL166C 0 322219 323565 ON 14 BNI5 complement(322219..323565) Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner YNL165W 1 323832 325052 ON 14 YNL165W (323832..325052) Putative protein of unknown function; YNL165W is not an essential gene YNL164C 0 325265 326320 ON 14 IBD2 complement(325265..326320) Component of the BUB2-dependent spindle checkpoint pathway, interacts with Bfa1p and functions upstream of Bub2p and Bfa1p YNL163C 0 326741 330073 ON 14 RIA1 complement(326741..330073) Cytoplasmic GTPase involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes YNL162W-A 1 330329 330547 ON 14 YNL162W-A (330329..330547) Putative protein of unknown function; identified by homology YNL162W 1 331322 332154 ON 14 RPL42A (331322..331325,331838..332154) Protein component of the large (60S) ribosomal subunit, identical to Rpl42Bp and has similarity to rat L44 ribosomal protein YNL161W 1 332597 334867 ON 14 CBK1 (332597..334867) Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization YNL160W 1 336545 337609 ON 14 YGP1 (336545..337609) Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p YNL159C 0 338478 339347 ON 14 ASI2 complement(338478..339347) Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals YNL158W 1 339612 340208 ON 14 PGA1 (339612..340208) Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes YNL157W 1 340352 340858 ON 14 IGO1 (340352..340858) Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p YNL156C 0 341069 341968 ON 14 NSG2 complement(341069..341968) Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins YNL155W 1 342516 343340 ON 14 YNL155W (342516..343340) Putative protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic YNL154C 0 343636 345276 ON 14 YCK2 complement(343636..345276) Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck1p YNL153C 0 345669 346058 ON 14 GIM3 complement(345669..346058) Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it YNL152W 1 346313 347542 ON 14 INN1 (346313..347542) Essential protein that associates with the contractile actomyosin ring, required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function YNL151C 0 347766 348521 ON 14 RPC31 complement(347766..348521) RNA polymerase III subunit C31 YNL150W 1 349251 349658 OFF 14 YNL150W (349251..349658) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking YNL149C 0 349367 349756 ON 14 PGA2 complement(349367..349756) Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect YNL148C 0 349907 350671 ON 14 ALF1 complement(349907..350671) Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance YNL147W 1 350940 351383 ON 14 LSM7 (350940..350957,351054..351383) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YNL146C-A 0 351386 351580 OFF 14 YNL146C-A complement(351386..351580) Putative protein of unknown function YNL146W 1 351715 352017 ON 14 YNL146W (351715..352017) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene YNL145W 1 352414 352530 ON 14 MFA2 (352414..352530) Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 YNL144C 0 352820 355042 ON 14 YNL144C complement(352820..355042) Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study YNL144W-A 1 353061 353144 OFF 14 YNL144W-A (353061..353144) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YNL144C YNL143C 0 356794 357186 OFF 14 YNL143C complement(356794..357186) Protein of unknown function; expressed at both mRNA and protein levels YNL142W 1 357453 358952 ON 14 MEP2 (357453..358952) Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation YNL141W 1 359596 360639 ON 14 AAH1 (359596..360639) Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome YNL140C 0 360917 361486 OFF 14 YNL140C complement(360917..361486) Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C YNL139C 0 360924 365717 ON 14 THO2 complement(360924..365717) Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids YNL138W-A 1 366033 366412 ON 14 YSF3 (366033..366035,366158..366412) Component of the SF3b subcomplex of the U2 snRNP, essential protein required for for splicing and for assembly of SF3b YNL138W 1 366741 368321 ON 14 SRV2 (366741..368321) CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis YNL137C 0 368595 370055 ON 14 NAM9 complement(368595..370055) Mitochondrial ribosomal component of the small subunit YNL136W 1 370368 371645 ON 14 EAF7 (370368..371645) Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A YNL135C 0 371882 372226 ON 14 FPR1 complement(371882..372226) Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function YNL134C 0 372451 373581 ON 14 YNL134C complement(372451..373581) Putative protein of unknown function with similarity to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced by the DNA-damaging agent MMS YNL133C 0 374171 374692 ON 14 FYV6 complement(374171..374692) Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Scer_14.trna4F 1 374869 374959 ON 14 tF-GAA (374869..374905,374924..374959) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 70.15 YNL132W 1 375321 378491 ON 14 KRE33 (375321..378491) Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance YNL131W 1 378767 379225 ON 14 TOM22 (378767..379225) Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes YNL130C 0 379558 380831 ON 14 CPT1 complement(379558..380689,380782..380831) Cholinephosphotransferase, required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription YNL130C-A 0 381245 381391 OFF 14 DGR1 complement(381245..381391) Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose YNL129W 1 381480 382202 ON 14 NRK1 (381480..382202) Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis YNL128W 1 382359 383663 ON 14 TEP1 (382359..383663) PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 YNL127W 1 383987 386848 ON 14 FAR11 (383987..386848) Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 YNL126W 1 387227 389767 ON 14 SPC98 (387227..389767) Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque YNL125C 0 390146 392167 ON 14 ESBP6 complement(390146..392167) Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane YNL124W 1 392892 394370 ON 14 NAF1 (392892..394370) RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p YNL123W 1 394685 397678 ON 14 NMA111 (394685..397678) Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases YNL122C 0 398023 398370 ON 14 YNL122C complement(398023..398370) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene YNL121C 0 398684 400537 ON 14 TOM70 complement(398684..400537) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins YNL120C 0 401033 401518 OFF 14 YNL120C complement(401033..401518) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene YNL119W 1 401040 402521 ON 14 NCS2 (401040..402521) Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae YNL118C 0 402652 405564 ON 14 DCP2 complement(402652..405564) Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; member of the Nudix hydrolase family YNL117W 1 406358 408022 ON 14 MLS1 (406358..408022) Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium YNL116W 1 408341 409909 ON 14 DMA2 (408341..409909) Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains YNL115C 0 410119 412053 ON 14 YNL115C complement(410119..412053) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene YNL114C 0 412684 413055 OFF 14 YNL114C complement(412684..413055) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit YNL113W 1 412771 413199 ON 14 RPC19 (412771..413199) RNA polymerase subunit AC19, common to RNA polymerases I and III YNL112W 1 413639 416281 ON 14 DBP2 (413639..414911,415914..416281) Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated mRNA decay and rRNA processing YNL111C 0 416940 417302 ON 14 CYB5 complement(416940..417302) Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation YNL110C 0 417826 418488 ON 14 NOP15 complement(417826..418488) Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm YNL109W 1 418964 419509 OFF 14 YNL109W (418964..419509) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C YNL108C 0 419014 419826 ON 14 YNL108C complement(419014..419826) Putative protein of unknown function with similarity to Tfc7p and prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus YNL107W 1 420098 420778 ON 14 YAF9 (420098..420778) Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain YNL106C 0 420944 424495 ON 14 INP52 complement(420944..424495) Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain YNL105W 1 424155 424583 OFF 14 RRT16 (424155..424583) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription YNL104C 0 424895 426754 ON 14 LEU4 complement(424895..426754) Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway YNL103W-A 1 426719 426808 OFF 14 YNL103W-A (426719..426808) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MET4/YNL104C YNL103W 1 427735 429753 ON 14 MET4 (427735..429753) Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p YNL102W 1 430087 434493 ON 14 POL1 (430087..434493) Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis YNL101W 1 434999 437140 ON 14 AVT4 (434999..437140) Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YNL100W 1 437613 438317 ON 14 AIM37 (437613..438317) Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture YNL099C 0 438567 439283 ON 14 OCA1 complement(438567..439283) Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA YNL098C 0 439602 440570 ON 14 RAS2 complement(439602..440570) GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes YNL097C-B 0 440797 440919 OFF 14 YNL097C-B complement(440797..440919) Putative protein of unknown function YNL097C 0 441366 442358 ON 14 PHO23 complement(441366..442358) Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 YNL097W-A 1 441431 441586 OFF 14 YNL097W-A (441431..441586) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene PHO23/YNL097C Scer_14.trna5L 1 443006 443119 ON 14 tL-CAA (443006..443043,443076..443119) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: CAA | Cove Score: 54.18 YNL096C 0 443398 444315 ON 14 RPS7B complement(443398..443826,444172..444315) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins YNL095C 0 444912 446840 ON 14 YNL095C complement(444912..446840) Putative protein of unknown function predicted to contain a transmembrane domain; YNL095C is not an essential gene YNL094W 1 447611 449374 ON 14 APP1 (447611..449374) Protein of unknown function, interacts with Rvs161p and Rvs167p; computational analysis of protein-protein interactions in large-scale studies suggests a possible role in actin filament organization YNL093W 1 449868 450530 ON 14 YPT53 (449868..450530) Rab family GTPase, similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis YNL092W 1 450871 452073 ON 14 YNL092W (450871..452073) Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene YNL091W 1 452408 456130 ON 14 NST1 (452408..456130) Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 YNL090W 1 456565 457143 ON 14 RHO2 (456565..457143) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly YNL089C 0 456693 457169 OFF 14 YNL089C complement(456693..457169) Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins YNL088W 1 457704 461990 ON 14 TOP2 (457704..461990) Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis YNL087W 1 462411 465947 ON 14 TCB2 (462411..465947) Bud-specific protein with a potential role in membrane trafficking; localizes to endoplasmic reticulum and is enriched at ER-plasma membrane contact sites; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud YNL086W 1 466334 466642 ON 14 SNN1 (466334..466642) Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes YNL085W 1 467131 469623 ON 14 MKT1 (467131..469623) Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more YNL084C 0 470053 471102 ON 14 END3 complement(470053..471102) EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p YNL083W 1 471377 472861 ON 14 SAL1 (471377..472861) ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains YNL082W 1 473391 476012 ON 14 PMS1 (473391..476012) ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL YNL081C 0 476188 476619 ON 14 SWS2 complement(476188..476619) Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency YNL080C 0 476932 478032 ON 14 EOS1 complement(476932..478032) Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene YNL079C 0 478566 479165 ON 14 TPM1 complement(478566..479165) Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently YNL078W 1 479768 480991 ON 14 NIS1 (479768..480991) Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network YNL077W 1 481391 482977 ON 14 APJ1 (481391..482977) Putative chaperone of the HSP40 (DNAJ) family; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNL076W 1 483556 485310 ON 14 MKS1 (483556..485310) Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling YNL075W 1 485607 486479 ON 14 IMP4 (485607..486479) Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs YNL074C 0 486766 488124 ON 14 MLF3 complement(486766..488124) Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide YNL073W 1 488386 490116 ON 14 MSK1 (488386..490116) Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) YNL072W 1 490317 491240 ON 14 RNH201 (490317..491240) Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome YNL071W 1 491523 492971 ON 14 LAT1 (491523..492971) Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA YNL070W 1 493366 493548 ON 14 TOM7 (493366..493548) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex YNL069C 0 493956 495001 ON 14 RPL16B complement(493956..494524,494974..495001) N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Ap, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p YNL068C 0 495701 498289 ON 14 FKH2 complement(495701..498289) Forkhead family transcription factor with a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase YNL067W-A 1 498538 498684 OFF 14 YNL067W-A (498538..498684) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL067W-B 1 499417 499557 OFF 14 YNL067W-B (499417..499557) Putative protein of unknown function YNL067W 1 499681 500256 ON 14 RPL9B (499681..500256) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins YNL066W 1 501515 502777 ON 14 SUN4 (501515..502777) Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family YNL065W 1 503724 505484 ON 14 AQR1 (503724..505484) Plasma membrane multidrug transporter of the major facilitator superfamily, confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids YNL064C 0 505868 507097 ON 14 YDJ1 complement(505868..507097) Type I HSP40 co-chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family YNL063W 1 507758 508702 ON 14 MTQ1 (507758..508702) S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene YNL062C 0 508776 510212 ON 14 GCD10 complement(508776..510212) Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression YNL061W 1 510540 512396 ON 14 NOP2 (510540..512396) Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles YNL059C 0 512668 514935 ON 14 ARP5 complement(512668..514935) Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes YNL058C 0 515763 516713 ON 14 YNL058C complement(515763..516713) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene YNL057W 1 516403 516735 OFF 14 YNL057W (516403..516735) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL056W 1 517249 517842 ON 14 OCA2 (517249..517842) Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene YNL055C 0 517994 518845 ON 14 POR1 complement(517994..518845) Mitochondrial porin (voltage-dependent anion channel), outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated Scer_14.trna6D 0 519099 519169 ON 14 tD-GUC complement(519099..519169) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 31.75 YNL054W-B 1 519456 524706 OFF 14 YNL054W-B (519456..520760,520762..524706) TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition YNL054W 1 526086 529583 ON 14 VAC7 (526086..529583) Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock YNL053W 1 529942 531411 ON 14 MSG5 (529942..531411) Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p YNL052W 1 531725 532186 ON 14 COX5A (531725..532186) Subunit Va of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth YNL051W 1 532659 533870 ON 14 COG5 (532659..533870) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YNL050C 0 534079 534982 ON 14 YNL050C complement(534079..534874,534966..534982) Putative protein of unknown function; YNL050c is not an essential gene YNL049C 0 535281 537911 ON 14 SFB2 complement(535281..537911) Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p YNL048W 1 538173 539819 ON 14 ALG11 (538173..539819) Alpha-1,2-mannosyltransferase, catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER YNL047C 0 539911 541881 ON 14 SLM2 complement(539911..541881) Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex YNL046W 1 542304 542822 ON 14 YNL046W (542304..542822) Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YNL045W 1 542963 544978 ON 14 LAP2 (542963..544978) Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YNL044W 1 545268 545877 ON 14 YIP3 (545268..545291,545371..545877) Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p YNL043C 0 545589 545909 OFF 14 YNL043C complement(545589..545909) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Scer_14.trna7P 1 547094 547196 ON 14 tP-UGG (547094..547129,547161..547196) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 59.08 YNL042W-B 1 547113 547370 OFF 14 YNL042W-B (547113..547370) Putative protein of unknown function YNL042W 1 548100 549290 ON 14 BOP3 (548100..549290) Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury YNL041C 0 549468 551987 ON 14 COG6 complement(549468..551987) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YNL040W 1 553380 554750 ON 14 YNL040W (553380..554750) Putative protein of unknown function with strong similarity to alanyl-tRNA synthases from Eubacteria; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene YNL039W 1 555048 556832 ON 14 BDP1 (555048..556832) Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs YNL038W 1 557020 557783 ON 14 GPI15 (557020..557610,557685..557783) Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein YNL037C 0 557920 559002 ON 14 IDH1 complement(557920..559002) Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle YNL036W 1 559814 560479 ON 14 NCE103 (559814..560479) Carbonic anhydrase; metalloenzyme that catalyzes CO2 hydration to bicarbonate, which is an important metabolic substrate, and protons; not expressed under conditions of high CO2, such as inside a growing colony, but transcription is induced in response to low CO2 levels, such as on the colony surface in ambient air; poorly transcribed under aerobic conditions and at an undetectable level under anaerobic conditions; mutation affects non-classical protein export pathway Scer_14.trna8T 0 560693 560765 ON 14 tT-AGU complement(560693..560765) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 Scer_14.trna9P 1 568115 568217 ON 14 tP-UGG (568115..568150,568182..568217) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 59.08 YNL035C 0 568522 569691 ON 14 YNL035C complement(568522..569691) Putative protein of unknown function with similarity to proteins containing WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene Scer_14.trna10I 0 569867 569940 ON 14 tI-AAU complement(569867..569940) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YNL034W 1 570477 572315 ON 14 YNL034W (570477..572315) Putative protein of unknown function; YNL034W is not an essential gene YNL033W 1 572999 573853 ON 14 YNL033W (572999..573853) Putative protein of unknown function YNL032W 1 574506 575351 ON 14 SIW14 (574506..575351) Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm YNL031C 0 575640 576050 ON 14 HHT2 complement(575640..576050) Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation YNL030W 1 576727 577038 ON 14 HHF2 (576727..577038) Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity YNL029C 0 577205 578773 ON 14 KTR5 complement(577205..578773) Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YNL028W 1 578679 578996 OFF 14 YNL028W (578679..578996) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YNL027W 1 579580 581616 ON 14 CRZ1 (579580..581616) Transcription factor that activates transcription of genes involved in stress response; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation YNL026W 1 581920 583374 ON 14 SAM50 (581920..583374) Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 YNL025C 0 584320 585291 ON 14 SSN8 complement(584320..585291) Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Scer_NME1 1 585587 585926 ON 14 NME1 (585587..585926) RNA component of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; human ortholog is implicated in cartilage-hair hypoplasia (CHH) Scer_snR66 1 586090 586175 ON 14 SNR66 (586090..586175) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U2417 YNL024C-A 0 586602 586820 ON 14 KSH1 complement(586602..586820) Essential protein suggested to function early in the secretory pathway; inviability is suppressed by overexpression of Golgi protein Tvp23p; ortholog of human Kish YNL024C 0 587107 587847 ON 14 YNL024C complement(587107..587847) Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern YNL023C 0 588263 591160 ON 14 FAP1 complement(588263..591160) Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 YNL022C 0 591427 592899 ON 14 YNL022C complement(591427..592899) Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis YNL021W 1 593227 595347 ON 14 HDA1 (593227..595347) Putative catalytic subunit of a class II histone deacetylase complex that also contains Hda2p and Hda3p; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation YNL020C 0 595623 597539 ON 14 ARK1 complement(595623..597539) Serine/threonine protein kinase involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis YNL019C 0 598376 599230 ON 14 YNL019C complement(598376..599230) Putative protein of unknown function YNL018C 0 599936 601774 ON 14 YNL018C complement(599936..601774) Putative protein of unknown function YNL017C 0 602138 602476 OFF 14 YNL017C complement(602138..602476) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the tRNA ORF tI(AAU)N2 Scer_14.trna11I 1 602312 602385 ON 14 tI-AAU (602312..602385) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YNL016W 1 602907 604268 ON 14 PUB1 (602907..604268) Poly (A)+ RNA-binding protein, abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly YNL015W 1 605384 605611 ON 14 PBI2 (605384..605611) Cytosolic inhibitor of vacuolar proteinase B (PRB1), required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion YNL014W 1 606319 609453 ON 14 HEF3 (606319..609453) Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells YNL013C 0 609133 609510 OFF 14 YNL013C complement(609133..609510) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W YNL012W 1 609686 611665 ON 14 SPO1 (609686..609790,609875..611665) Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B YNL011C 0 611836 613170 ON 14 YNL011C complement(611836..613170) Putative protein of unknown function; YNL011C is not an essential gene YNL010W 1 613635 614360 ON 14 YNL010W (613635..614360) Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation YNL009W 1 614821 616083 ON 14 IDP3 (614821..616083) Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids YNL008C 0 616211 618241 ON 14 ASI3 complement(616211..618241) Putative integral membrane E3 ubiquitin ligase; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals YNL007C 0 618507 619565 ON 14 SIS1 complement(618507..619565) Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins YNL006W 1 620067 620978 ON 14 LST8 (620067..620978) Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat YNL005C 0 621313 622428 ON 14 MRP7 complement(621313..622428) Mitochondrial ribosomal protein of the large subunit YNL004W 1 622915 624621 ON 14 HRB1 (622915..622944,623287..624621) Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p YNL003C 0 624975 625829 ON 14 PET8 complement(624975..625829) S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth YNL002C 0 626174 627142 ON 14 RLP7 complement(626174..627142) Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs YNL001W 1 627456 628616 ON 14 DOM34 (627456..628616) Protein that, with binding partner Hbs1p, facilitates ribosomal subunit dissociation when translation is stalled; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog Scer_CEN14 1 628758 628875 ON 14 CEN14 (628758..628875) Chromosome XIV centromere YNR001C 0 629622 631061 ON 14 CIT1 complement(629622..631061) Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein YNR001W-A 1 631263 631481 OFF 14 YNR001W-A (631263..631481) Dubious open reading frame unlikely to encode a functional protein; identified by homology Scer_14.trna12P 0 631846 631917 ON 14 tP-AGG complement(631846..631917) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: AGG | Cove Score: 66.99 Scer_14.trna13N 1 632599 632672 ON 14 tN-GUU (632599..632672) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YNR002C 0 633008 633856 ON 14 ATO2 complement(633008..633856) Putative transmembrane protein involved in export of ammonia, a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Ady2p and Y. lipolytica Gpr1p YNR003C 0 634344 635297 ON 14 RPC34 complement(634344..635297) RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex YNR003W-A 1 634420 634518 OFF 14 YNR003W-A (634420..634518) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene RPC34/YNR003C YNR004W 1 635943 636383 ON 14 SWM2 (635943..636383) Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds YNR005C 0 636931 637335 OFF 14 YNR005C complement(636931..637335) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YNR006W 1 636986 638854 ON 14 VPS27 (636986..638854) Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) YNR007C 0 639182 640114 ON 14 ATG3 complement(639182..640114) E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt YNR008W 1 640396 642381 ON 14 LRO1 (640396..642381) Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase YNR009W 1 642690 643439 ON 14 NRM1 (642690..643439) Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase YNR010W 1 643744 644193 ON 14 CSE2 (643744..644193) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity YNR011C 0 644320 646950 ON 14 PRP2 complement(644320..646950) RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 YNR012W 1 647432 648937 ON 14 URK1 (647432..648937) Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP YNR013C 0 649028 651712 ON 14 PHO91 complement(649028..651712) Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth YNR014W 1 652465 653103 ON 14 YNR014W (652465..653103) Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible YNR015W 1 653387 654541 ON 14 SMM1 (653387..654541) Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs YNR016C 0 654673 661374 ON 14 ACC1 complement(654673..661374) Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids YNR017W 1 662913 663581 ON 14 TIM23 (662913..663581) Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel YNR018W 1 664270 664944 ON 14 RCF2 (664270..664944) Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast YNR019W 1 665339 667267 ON 14 ARE2 (665339..667267) Acyl-CoA:sterol acyltransferase, isozyme of Are1p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen YNR020C 0 667410 668222 ON 14 ATP23 complement(667410..668222) Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex YNR021W 1 668377 669591 ON 14 YNR021W (668377..669591) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene YNR022C 0 669774 670193 ON 14 MRPL50 complement(669774..670193) Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation YNR023W 1 670418 672118 ON 14 SNF12 (670418..672118) 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; deletion mutants are temperature-sensitive YNR024W 1 672409 672969 ON 14 MPP6 (672409..672969) Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes YNR025C 0 672702 673061 OFF 14 YNR025C complement(672702..673061) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase YNR026C 0 673274 674689 ON 14 SEC12 complement(673274..674689) Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER YNR027W 1 674923 675876 ON 14 BUD17 (674923..675876) Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) YNR028W 1 676177 677103 ON 14 CPR8 (676177..677103) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway YNR029C 0 677199 678488 ON 14 YNR029C complement(677199..678488) Putative protein of unknown function, deletion confers reduced fitness in saline YNR030W 1 678799 680454 ON 14 ALG12 (678799..680454) Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation YNR031C 0 680694 685433 ON 14 SSK2 complement(680694..685433) MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress YNR032W 1 686010 687116 ON 14 PPG1 (686010..687116) Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases YNR032C-A 0 687243 687464 ON 14 HUB1 complement(687243..687464) Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear YNR033W 1 687635 689998 ON 14 ABZ1 (687635..689998) Para-aminobenzoate (PABA) synthase, has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis YNR034W 1 690321 691286 ON 14 SOL1 (690321..691286) Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol2p, Sol3p, and Sol4p YNR034W-A 1 692561 692857 ON 14 YNR034W-A (692561..692857) Putative protein of unknown function; expression is regulated by Msn2p/Msn4p YNR035C 0 693019 694047 ON 14 ARC35 complement(693019..694047) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin YNR036C 0 694361 694822 ON 14 MRPS12 complement(694361..694822) Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins YNR037C 0 695052 695327 ON 14 RSM19 complement(695052..695327) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein YNR038W 1 695595 697484 ON 14 DBP6 (695595..697484) Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family YNR039C 0 697614 699431 ON 14 ZRG17 complement(697614..699431) Endoplasmic reticulum protein of unknown function, transcription is induced under conditions of zinc deficiency; mutant phenotype suggests a role in uptake of zinc YNR040W 1 699690 700460 ON 14 YNR040W (699690..700460) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNR041C 0 700541 701659 ON 14 COQ2 complement(700541..701659) Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis YNR042W 1 701238 701666 OFF 14 YNR042W (701238..701666) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 YNR043W 1 701895 703085 ON 14 MVD1 (701895..703085) Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer YNR044W 1 703699 705876 ON 14 AGA1 (703699..705876) Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds YNR045W 1 706139 707608 ON 14 PET494 (706139..707608) Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane YNR046W 1 707788 708195 ON 14 TRM112 (707788..708195) Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin YNR047W 1 708523 711204 ON 14 FPK1 (708523..711204) Ser/Thr protein kinase that regulates the putative phospholipid translocases Lem3p-Dnf1p/Dnf2p; phosphorylates and inhibits upstream inhibitory kinase, Ypk1p; localizes to the cytoplasm, early endosome/TGN compartments, and plasma membrane YNR048W 1 711630 712811 ON 14 YNR048W (711630..712811) Protein that interacts specifically in vivo with phospholipid translocase (flippase) Dnf3p; similar to Cdc50p, which is an essential interaction partner of the flippase Drs2p YNR049C 0 713023 713655 ON 14 MSO1 complement(713023..713655) Probable component of the secretory vesicle docking complex, acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation YNR050C 0 714048 715388 ON 14 LYS9 complement(714048..715388) Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Scer_snR49 1 716120 716284 ON 14 SNR49 (716120..716284) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U990 and small subunit (SSU) rRNA at positions U120, U211, and U302 YNR051C 0 716780 718327 ON 14 BRE5 complement(716780..718327) Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A YNR052C 0 719346 720647 ON 14 POP2 complement(719346..720647) RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation YNR053C 0 721120 723112 ON 14 NOG2 complement(721120..721770,722303..723112) Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Scer_snR191 0 721938 722211 OFF 14 SNR191 complement(721938..722211) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2258 and U2260; encoded within the intron of NOG2/YNR053C YNR054C 0 723356 724306 ON 14 ESF2 complement(723356..724306) Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome Scer_14.trna14L 0 726134 726217 ON 14 tL-UAA complement(726134..726217) Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; tRNA Type: Leu (L) | Anti-Codon: UAA | Cove Score: 69.31 YNR055C 0 728426 730186 ON 14 HOL1 complement(728426..730186) Putative transporter in the major facilitator superfamily (DHA1 family) of multidrug resistance transporters; mutations in membrane-spanning domains permit cation and histidinol uptake YNR056C 0 731618 733303 ON 14 BIO5 complement(731618..733303) Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis YNR057C 0 733356 734069 ON 14 BIO4 complement(733356..734069) Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels YNR058W 1 734291 735733 ON 14 BIO3 (734291..735733) 7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis YNR059W 1 736803 738545 ON 14 MNT4 (736803..738545) Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation YNR060W 1 739951 742110 ON 14 FRE4 (739951..742110) Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels YNR061C 0 742881 743540 ON 14 YNR061C complement(742881..743540) Putative protein of unknown function YNR062C 0 744360 745343 ON 14 YNR062C complement(744360..745343) Putative membrane protein of unknown function YNR063W 1 746943 748766 ON 14 YNR063W (746943..748766) Putative zinc-cluster protein of unknown function YNR064C 0 749136 750008 ON 14 YNR064C complement(749136..750008) Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides YNR065C 0 750350 753700 ON 14 YNR065C complement(750350..753700) Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene YNR066C 0 753725 755035 ON 14 YNR066C complement(753725..755035) Putative membrane-localized protein of unknown function YNR067C 0 755746 759099 ON 14 DSE4 complement(755746..759099) Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother YNR068C 0 760067 760885 ON 14 YNR068C complement(760067..760885) Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form YNR069C 0 761123 762592 ON 14 BSC5 complement(761123..762592) Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro YNR070W 1 765375 769376 ON 14 PDR18 (765375..769376) Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YNR071C 0 770440 771468 ON 14 YNR071C complement(770440..771468) Putative protein of unknown function YNR072W 1 772657 774351 ON 14 HXT17 (772657..774351) Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose YNR073C 0 774792 776300 ON 14 YNR073C complement(774792..776300) Putative mannitol dehydrogenase YNR074C 0 777602 778738 ON 14 AIF1 complement(777602..778738) Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase YNR075W 1 779916 781040 ON 14 COS10 (779916..781040) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YNR075C-A 0 781511 781603 OFF 14 YNR075C-A complement(781511..781603) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YNR076W 1 781918 782280 ON 14 PAU6 (781918..782280) Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme; identical to Pau18p YNR077C 0 783287 783541 OFF 14 YNR077C complement(783287..783541) Protein of unknown function, abundance changes with carbon source YOL166W-A 1 585 740 OFF 15 YOL166W-A (585..740) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOL166C 0 1000 1338 OFF 15 YOL166C complement(1000..1338) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOL165C 0 1647 2078 ON 15 AAD15 complement(1647..2078) Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YOL164W-A 1 4130 4312 OFF 15 YOL164W-A (4130..4312) Putative protein of unknown function; identified by fungal homology and RT-PCR YOL164W 1 6175 8115 ON 15 BDS1 (6175..8115) Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources YOL163W 1 9597 10106 ON 15 YOL163W (9597..10106) Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family YOL162W 1 10119 10766 ON 15 YOL162W (10119..10766) Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family YOL161C 0 11549 11911 ON 15 PAU20 complement(11549..11911) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression induced by low temperature and also by anaerobic conditions; induced during alcoholic fermentation YOL160W 1 14313 14654 OFF 15 YOL160W (14313..14654) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOL159C-A 0 15233 15505 ON 15 YOL159C-A complement(15233..15505) Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species YOL159C 0 17281 17796 ON 15 YOL159C complement(17281..17796) Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA YOL158C 0 19491 21311 ON 15 ENB1 complement(19491..21311) Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment YOL157C 0 22525 24294 ON 15 IMA2 complement(22525..24294) Alpha-glucosidase with specificity for isomaltase, methyl-alpha-glucoside, and palatinose; member of IMA isomaltase family; not required for isomaltose utilization, but Ima2p overexpression allows the ima1 null mutant to grow on isomaltose YOL156W 1 25273 26976 ON 15 HXT11 (25273..26976) Putative hexose transporter that is nearly identical to Hxt9p, has similarity to major facilitator superfamily (MFS) transporters and is involved in pleiotropic drug resistance YOL155W-A 1 27084 27218 OFF 15 YOL155W-A (27084..27218) Putative protein of unknown function; identified by expression profiling and mass spectrometry YOL155C 0 28703 31606 ON 15 HPF1 complement(28703..31606) Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines YOL154W 1 34658 35407 ON 15 ZPS1 (34658..35407) Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH YOL153C 0 36822 38567 ON 15 YOL153C complement(36822..38567) Hypothetical protein YOL152W 1 40748 42610 ON 15 FRE7 (40748..42610) Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels YOL151W 1 43694 44722 ON 15 GRE2 (43694..44722) 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway YOL150C 0 44473 44784 OFF 15 YOL150C complement(44473..44784) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOL149W 1 44938 45633 ON 15 DCP1 (44938..45633) Subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p YOL148C 0 45760 47574 ON 15 SPT20 complement(45760..47574) Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay YOL147C 0 47933 48643 ON 15 PEX11 complement(47933..48643) Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p YOL146W 1 48864 49448 ON 15 PSF3 (48864..49448) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YOL145C 0 49555 52788 ON 15 CTR9 complement(49555..52788) Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats YOL144W 1 53098 54552 ON 15 NOP8 (53098..54552) Nucleolar protein required for 60S ribosomal subunit biogenesis YOL143C 0 54595 55104 ON 15 RIB4 complement(54595..55104) Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin YOL142W 1 55558 56280 ON 15 RRP40 (55558..56280) Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain both S1 and KH RNA binding domains; has similarity to human hRrp40p (EXOSC3) YOL141W 1 56452 58539 ON 15 PPM2 (56452..58539) AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p YOL140W 1 58759 60030 ON 15 ARG8 (58759..60030) Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine YOL139C 0 60383 61024 ON 15 CDC33 complement(60383..61024) Cytoplasmic mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p) YOL138C 0 61325 65350 ON 15 RTC1 complement(61325..65350) Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif YOL137W 1 65621 67114 ON 15 BSC6 (65621..67114) Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression YOL136C 0 67561 68754 ON 15 PFK27 complement(67561..68754) 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A YOL135C 0 69376 70044 ON 15 MED7 complement(69376..70044) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YOL134C 0 70156 70545 OFF 15 YOL134C complement(70156..70545) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit YOL133W 1 70325 70690 ON 15 HRT1 (70325..70690) RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF YOL132W 1 71300 72715 ON 15 GAS4 (71300..72715) 1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall YOL131W 1 73031 73357 ON 15 YOL131W (73031..73357) Putative protein of unknown function YOL130W 1 74400 76979 ON 15 ALR1 (74400..76979) Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions YOL129W 1 77560 78114 ON 15 VPS68 (77560..78114) Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria YOL128C 0 78352 79479 ON 15 YGK3 complement(78352..79479) Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation YOL127W 1 80348 81190 ON 15 RPL25 (80348..80360,80775..81190) Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarity to E. coli L23 and rat L23a ribosomal proteins; binds to 25S rRNA via a conserved C-terminal motif YOL126C 0 81787 82920 ON 15 MDH2 complement(81787..82920) Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 YOL125W 1 83834 85264 ON 15 TRM13 (83834..85264) 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases YOL124C 0 85456 86757 ON 15 TRM11 complement(85456..86757) Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain YOL123W 1 87844 89448 ON 15 HRP1 (87844..89448) Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences YOL122C 0 89692 91419 ON 15 SMF1 complement(89692..91419) Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins YOL121C 0 92026 92850 ON 15 RPS19A complement(92026..92440,92831..92850) Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19Bp YOL120C 0 93395 94402 ON 15 RPL18A complement(93395..93843,94291..94402) Protein component of the large (60S) ribosomal subunit, identical to Rpl18Bp and has similarity to rat L18 ribosomal protein; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation YOL119C 0 94856 96361 ON 15 MCH4 complement(94856..96361) Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport YOL118C 0 96608 96916 OFF 15 YOL118C complement(96608..96916) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOL117W 1 97551 99488 ON 15 RRI2 (97551..99488) Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response YOL116W 1 99809 100957 ON 15 MSN1 (99809..100957) Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus YOL115W 1 101475 103229 ON 15 PAP2 (101475..103229) Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p YOL114C 0 103317 103925 ON 15 YOL114C complement(103317..103925) Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene YOL113W 1 104326 106293 ON 15 SKM1 (104326..106293) Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth YOL112W 1 106710 108188 ON 15 MSB4 (106710..108188) GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization YOL111C 0 108258 108896 ON 15 MDY2 complement(108258..108896) Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes YOL110W 1 109176 109889 ON 15 SHR5 (109176..109889) Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization YOL109W 1 110297 110638 ON 15 ZEO1 (110297..110638) Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Scer_15.trna1G 0 110962 111033 ON 15 tG-UCC complement(110962..111033) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: UCC | Cove Score: 66.72 YOL108C 0 111431 111886 ON 15 INO4 complement(111431..111886) Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain YOL107W 1 112102 113130 ON 15 YOL107W (112102..113130) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein YOL106W 1 113226 113579 OFF 15 YOL106W (113226..113579) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_15.trna2T 1 113802 113874 ON 15 tT-AGU (113802..113874) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YOL105C 0 114138 115808 ON 15 WSC3 complement(114138..115808) Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis YOL104C 0 116396 117454 ON 15 NDJ1 complement(116396..117454) Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement YOL103W 1 124001 125830 ON 15 ITR2 (124001..125830) Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively YOL102C 0 125997 126689 ON 15 TPT1 complement(125997..126689) tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide YOL101C 0 126982 127920 ON 15 IZH4 complement(126982..127920) Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism YOL100W 1 129237 132482 ON 15 PKH2 (129237..132482) Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p YOL099C 0 132017 132508 OFF 15 YOL099C complement(132017..132508) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes YOL098C 0 132725 135838 ON 15 YOL098C complement(132725..135838) Putative metalloprotease Scer_snR58 0 136088 136183 ON 15 SNR58 complement(136088..136183) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position C663 YOL097W-A 1 136220 136405 OFF 15 YOL097W-A (136220..136405) Putative protein of unknown function; identified by expression profiling and mass spectrometry YOL097C 0 136527 137825 ON 15 WRS1 complement(136527..137825) Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA YOL096C 0 138107 139045 ON 15 COQ3 complement(138107..139045) O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein YOL095C 0 139227 141347 ON 15 HMI1 complement(139227..141347) Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD YOL094C 0 141584 142555 ON 15 RFC4 complement(141584..142555) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YOL093W 1 142815 143696 ON 15 TRM10 (142815..143696) tRNA methyltransferase, methylates the N-1 position of guanosine in tRNAs YOL092W 1 144204 145130 ON 15 YOL092W (144204..145130) Putative protein of unknown function; predicted to contain six transmembrane domains and is 58% similar to the uncharacterized ORF YBR147W YOL091W 1 145334 147163 ON 15 SPO21 (145334..147163) Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation YOL090W 1 147382 150276 ON 15 MSH2 (147382..150276) Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity; Msh2p-Msh6p binds to and hydrolyzes ATP YOL089C 0 150398 153490 ON 15 HAL9 complement(150398..153490) Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression YOL088C 0 153912 154745 ON 15 MPD2 complement(153912..154745) Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p YOL087C 0 155287 158637 ON 15 DUF1 complement(155287..158637) Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid YOL086W-A 1 159173 159445 ON 15 MHF1 (159173..159445) Protein of unknown function; ortholog of human MHF1, and a component of the heterotetrameric MHF histone-fold complex that in humans interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive YOL086C 0 159548 160594 ON 15 ADH1 complement(159548..160594) Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway YOL085W-A 1 161579 161791 OFF 15 YOL085W-A (161579..161791) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YOL085C YOL085C 0 161673 162014 OFF 15 YOL085C complement(161673..162014) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A YOL084W 1 162356 165331 ON 15 PHM7 (162356..165331) Protein of unknown function, expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole YOL083W 1 165714 166952 ON 15 ATG34 (165714..166952) Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog YOL083C-A 0 166461 166601 OFF 15 YOL083C-A complement(166461..166601) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry YOL082W 1 168727 169974 ON 15 ATG19 (168727..169974) Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles YOL081W 1 171070 180309 ON 15 IRA2 (171070..180309) GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin YOL080C 0 180558 181427 ON 15 REX4 complement(180558..181427) Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly YOL079W 1 181057 181455 OFF 15 YOL079W (181057..181455) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOL078W 1 181682 185212 ON 15 AVO1 (181682..185212) Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth YOL077W-A 1 185438 185644 ON 15 ATP19 (185438..185644) Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase YOL077C 0 185848 186723 ON 15 BRX1 complement(185848..186723) Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif YOL076W 1 187024 189414 ON 15 MDM20 (187024..189414) Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly YOL075C 0 189658 193542 ON 15 YOL075C complement(189658..193542) Putative ABC transporter YOL073C 0 193832 194800 ON 15 YOL073C complement(193832..194800) Putative protein of unknown function YOL072W 1 194970 196337 ON 15 THP1 (194970..196337) Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding YOL071W 1 196507 196995 ON 15 EMI5 (196507..196995) Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) YOL070C 0 197220 198725 ON 15 NBA1 complement(197220..198725) Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate YOL069W 1 198942 200297 ON 15 NUF2 (198942..200297) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering YOL068C 0 200368 201879 ON 15 HST1 complement(200368..201879) NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance YOL067C 0 201985 202518 ON 15 RTG1 complement(201985..202518) Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus YOL066C 0 202696 204471 ON 15 RIB2 complement(202696..204471) Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs YOL065C 0 204731 205885 ON 15 INP54 complement(204731..205885) Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins YOL064C 0 206103 207176 ON 15 MET22 complement(206103..207176) Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway YOL063C 0 207392 210265 ON 15 CRT10 complement(207392..210265) Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat YOL062C 0 210520 211995 ON 15 APM4 complement(210520..211995) Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport YOL061W 1 212244 213734 ON 15 PRS5 (212244..213734) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YOL060C 0 214017 216137 ON 15 MAM3 complement(214017..216137) Protein required for normal mitochondrial morphology, has similarity to hemolysins YOL059W 1 217126 218448 ON 15 GPD2 (217126..218448) NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria YOL058W 1 219211 220473 ON 15 ARG1 (219211..220473) Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate YOL057W 1 220767 222902 ON 15 YOL057W (220767..222902) Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers YOL056W 1 223268 224179 ON 15 GPM3 (223268..224179) Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event YOL055C 0 224420 226075 ON 15 THI20 complement(224420..226075) Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Scer_15.trna3G 0 226611 226681 ON 15 tG-GCC complement(226611..226681) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 Scer_15.trna4N 1 228331 228404 ON 15 tN-GUU (228331..228404) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YOL054W 1 228614 229834 ON 15 PSH1 (228614..229834) E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p YOL053W 1 230085 231272 ON 15 AIM39 (230085..231272) Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss YOL052C-A 0 231570 231755 ON 15 DDR2 complement(231570..231755) Multistress response protein, expression is activated by a variety of xenobiotic agents and environmental or physiological stresses YOL052C 0 232446 233636 ON 15 SPE2 complement(232446..233636) S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Scer_snR81 1 234346 234546 ON 15 SNR81 (234346..234546) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1052, constitutive pseudouridylation of U2 snRNA (LSR1) at position 42, and stress-induced U2 pseudouridylation at position 93 YOL051W 1 234940 238185 ON 15 GAL11 (234940..238185) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator YOL050C 0 237882 238202 OFF 15 YOL050C complement(237882..238202) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YOL049W 1 238619 240094 ON 15 GSH2 (238619..240094) Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock YOL048C 0 240204 241310 ON 15 RRT8 complement(240204..240947,241026..241310) Putative protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles YOL047C 0 241614 242747 ON 15 YOL047C complement(241614..242441,242505..242747) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YOL046C 0 243466 244140 OFF 15 YOL046C complement(243466..244140) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W YOL045W 1 243497 246802 ON 15 PSK2 (243497..246802) One of two (see also PSK1) PAS domain containing S/T protein kinases; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation) YOL044W 1 247150 248301 ON 15 PEX15 (247150..248301) Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly YOL043C 0 248392 249534 ON 15 NTG2 complement(248392..249534) DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus; sumoylated YOL042W 1 249825 250916 ON 15 NGL1 (249825..250916) Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOL041C 0 251267 252646 ON 15 NOP12 complement(251267..252646) Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p YOL040C 0 253149 253577 ON 15 RPS15 complement(253149..253577) Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ribosomal proteins YOL039W 1 254297 254617 ON 15 RPP2A (254297..254617) Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm YOL038C-A 0 254926 255021 OFF 15 YOL038C-A complement(254926..255021) Putative protein of unknown function; identified by SAGE analysis YOL038W 1 255337 256101 ON 15 PRE6 (255337..256101) Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress YOL037C 0 256666 257019 OFF 15 YOL037C complement(256666..257019) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W YOL036W 1 256745 259030 ON 15 YOL036W (256745..259030) Protein of unknown function; potential Cdc28p substrate YOL035C 0 258803 259105 OFF 15 YOL035C complement(258803..259105) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_snR50 1 259489 259578 ON 15 SNR50 (259489..259578) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position G867 YOL034W 1 259923 263204 ON 15 SMC5 (259923..263204) Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair YOL033W 1 263475 265085 ON 15 MSE1 (263475..265085) Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated YOL032W 1 265429 266169 ON 15 OPI10 (265429..266169) Protein with a possible role in phospholipid biosynthesis, based on inositol-excreting phenotype of the null mutant and its suppression by exogenous choline YOL031C 0 266264 267529 ON 15 SIL1 complement(266264..267529) Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein YOL030W 1 268187 269641 ON 15 GAS5 (268187..269641) 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall YOL029C 0 269815 270420 OFF 15 YOL029C complement(269815..270420) Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens YOL028C 0 270633 271370 ON 15 YAP7 complement(270633..271370) Putative basic leucine zipper (bZIP) transcription factor YOL027C 0 272003 273724 ON 15 MDM38 complement(272003..273724) Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome YOL026C 0 274012 274353 ON 15 MIM1 complement(274012..274353) Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Scer_15.trna5S 1 274673 274773 ON 15 tS-GCU (274673..274709,274729..274773) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; tRNA Type: Ser (S) | Anti-Codon: GCU | Cove Score: 68.67 YOL025W 1 274957 276939 ON 15 LAG2 (274957..276939) Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 YOL024W 1 277086 277604 ON 15 YOL024W (277086..277604) Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site YOL023W 1 278056 280086 ON 15 IFM1 (278056..280086) Mitochondrial translation initiation factor 2 YOL022C 0 280272 281498 ON 15 TSR4 complement(280272..281498) Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Scer_15.trna6G 1 282164 282234 ON 15 tG-GCC (282164..282234) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YOL021C 0 282421 285426 ON 15 DIS3 complement(282421..285426) Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3 YOL020W 1 286172 287950 ON 15 TAT2 (286172..287950) High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Scer_15.trna7Y 1 288192 288280 ON 15 tY-GUA (288192..288230,288245..288280) Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Tyr (Y) | Anti-Codon: GUA | Cove Score: 68.81 YOL019W-A 1 288420 288572 OFF 15 YOL019W-A (288420..288572) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOL019W 1 288898 290553 ON 15 YOL019W (288898..290553) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole YOL018C 0 290881 292074 ON 15 TLG2 complement(290881..292074) Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) YOL017W 1 292529 294673 ON 15 ESC8 (292529..294673) Protein involved in telomeric and mating-type locus silencing, interacts with Sir2p and also interacts with the Gal11p, which is a component of the RNA pol II mediator complex YOL016C 0 294777 296120 ON 15 CMK2 complement(294777..296120) Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk1p and mammalian Cam Kinase II YOL015W 1 297078 298838 ON 15 IRC10 (297078..298838) Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci YOL014W 1 299693 300067 ON 15 YOL014W (299693..300067) Putative protein of unknown function YOL013W-B 1 300690 300980 OFF 15 YOL013W-B (300690..300980) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element YOL013W-A 1 301047 301238 OFF 15 YOL013W-A (301047..301238) Putative protein of unknown function; identified by SAGE Scer_15.trna8P 1 301097 301198 ON 15 tP-UGG (301097..301132,301163..301198) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 58.94 YOL013C 0 301380 303035 ON 15 HRD1 complement(301380..303035) Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon YOL012C 0 303579 303983 ON 15 HTZ1 complement(303579..303983) Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin YOL011W 1 305349 307409 ON 15 PLB3 (305349..307409) Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro YOL010W 1 307938 309041 ON 15 RCL1 (307938..309041) Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected YOL009C 0 309324 310139 ON 15 MDM12 complement(309324..310139) Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins YOL008W 1 310312 310935 ON 15 COQ10 (310312..310935) Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes YOL007C 0 311342 312367 ON 15 CSI2 complement(311342..312367) Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene YOL006C 0 313078 315387 ON 15 TOP1 complement(313078..315387) Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination YOL005C 0 315813 316175 ON 15 RPB11 complement(315813..316175) RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit YOL004W 1 316938 321548 ON 15 SIN3 (316938..321548) Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity YOL003C 0 321858 322994 ON 15 PFA4 complement(321858..322994) Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases YOL002C 0 323411 324364 ON 15 IZH2 complement(323411..324364) Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc YOL001W 1 325249 326130 ON 15 PHO80 (325249..326130) Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Scer_CEN15 0 326584 326702 ON 15 CEN15 complement(326584..326702) Chromosome XV centromere YOR001W 1 326832 329033 ON 15 RRP6 (326832..329033) Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay YOR002W 1 329417 331051 ON 15 ALG6 (329417..331051) Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease YOR003W 1 331455 332891 ON 15 YSP3 (331455..332891) Putative precursor to the subtilisin-like protease III YOR004W 1 333592 334356 ON 15 UTP23 (333592..334356) Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein YOR005C 0 334509 337343 ON 15 DNL4 complement(334509..337343) DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth YOR006C 0 337680 338621 ON 15 TSR3 complement(337680..338621) Protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YOR007C 0 338938 339978 ON 15 SGT2 complement(338938..339978) Glutamine-rich cytoplasmic protein that serves as a scaffold for binding Get4/5p and other proteins required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; has similarity to human cochaperone SGT Scer_15.trna9R 0 340299 340371 ON 15 tR-ACG complement(340299..340371) Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine; tRNA Type: Arg (R) | Anti-Codon: ACG | Cove Score: 71.46 YOR008C 0 341278 342414 ON 15 SLG1 complement(341278..342414) Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response YOR008C-A 0 342857 343081 OFF 15 YOR008C-A complement(342857..343081) Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres YOR008W-B 1 343929 344030 OFF 15 YOR008W-B (343929..344030) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOR009W 1 344335 345798 ON 15 TIR4 (344335..345798) Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock YOR010C 0 346195 346950 ON 15 TIR2 complement(346195..346950) Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis YOR011W 1 349679 353863 ON 15 AUS1 (349679..353863) Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Scer_15.trna10T 0 354041 354113 ON 15 tT-AGU complement(354041..354113) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: AGU | Cove Score: 78.63 YOR011W-A 1 355652 355858 OFF 15 YOR011W-A (355652..355858) Putative protein of unknown function YOR012W 1 356543 356956 ON 15 YOR012W (356543..356956) Putative protein of unknown function YOR013W 1 356751 357221 OFF 15 IRC11 (356751..357221) Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci YOR014W 1 357674 359947 ON 15 RTS1 (357674..359947) B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in absence of Cdc55p; homolog of the mammalian B' subunit of PP2A YOR015W 1 359993 360352 OFF 15 YOR015W (359993..360352) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOR016C 0 360461 361084 ON 15 ERP4 complement(360461..361084) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport YOR017W 1 361412 363814 ON 15 PET127 (361412..363814) Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane YOR018W 1 364369 366882 ON 15 ROD1 (364369..366882) Membrane protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene,zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins YOR019W 1 368127 370319 ON 15 YOR019W (368127..370319) Protein of unknown function that may interact with ribosomes, based on co-purification experiments YOR020C 0 370524 370844 ON 15 HSP10 complement(370524..370844) Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES YOR020W-A 1 371685 371957 ON 15 YOR020W-A (371685..371957) Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOR021C 0 372798 373439 ON 15 SFM1 complement(372798..373439) SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism YOR022C 0 373710 375857 ON 15 YOR022C complement(373710..375857) Protein with similarity to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOR023C 0 376012 377712 ON 15 AHC1 complement(376012..377712) Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex YOR024W 1 377847 378170 OFF 15 YOR024W (377847..378170) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOR025W 1 378219 379562 ON 15 HST3 (378219..379562) Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism YOR026W 1 379781 380806 ON 15 BUB3 (379781..380806) Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p YOR027W 1 381053 382822 ON 15 STI1 (381053..382822) Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop YOR028C 0 383533 384420 ON 15 CIN5 complement(383533..384420) Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions YOR029W 1 384600 384935 OFF 15 YOR029W (384600..384935) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOR030W 1 386825 388684 ON 15 DFG16 (386825..388684) Probable multiple transmembrane protein, involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; required for accumulation of processed Rim101p YOR031W 1 389213 389422 ON 15 CRS5 (389213..389422) Copper-binding metallothionein, required for wild-type copper resistance YOR032C 0 389771 391075 ON 15 HMS1 complement(389771..391075) Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant YOR032W-A 1 392176 392376 OFF 15 YOR032W-A (392176..392376) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR033C 0 392416 394524 ON 15 EXO1 complement(392416..394524) 5'-3' exonuclease and flap-endonuclease involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains YOR034C 0 394837 397086 ON 15 AKR2 complement(394837..397086) Ankyrin repeat-containing protein similar to Akr1p; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p YOR034C-A 0 397426 397668 OFF 15 YOR034C-A complement(397426..397668) Putative protein of unknown function; identified by expression profiling and mass spectrometry YOR035C 0 397735 400104 ON 15 SHE4 complement(397735..400104) Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization YOR036W 1 400348 401214 ON 15 PEP12 (400348..401214) Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin YOR037W 1 401555 402655 ON 15 CYC2 (401555..402655) Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) YOR038C 0 402761 405388 ON 15 HIR2 complement(402761..405388) Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p YOR039W 1 405768 406544 ON 15 CKB2 (405768..406544) Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase YOR040W 1 407064 407921 ON 15 GLO4 (407064..407921) Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Scer_snR9 0 407948 408134 ON 15 SNR9 complement(407948..408134) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2340 YOR041C 0 408358 408789 OFF 15 YOR041C complement(408358..408789) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance YOR042W 1 408425 409660 ON 15 CUE5 (408425..409660) Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Scer_snR62 0 409765 409864 ON 15 SNR62 complement(409765..409864) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U1888 YOR043W 1 410870 412330 ON 15 WHI2 (410870..412330) Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression YOR044W 1 413007 413480 ON 15 IRC23 (413007..413480) Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci YOR045W 1 413852 414037 ON 15 TOM6 (413852..414037) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex YOR046C 0 414459 415907 ON 15 DBP5 complement(414459..415907) Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p YOR047C 0 416347 417681 ON 15 STD1 complement(416347..417681) Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] YOR048C 0 418630 421650 ON 15 RAT1 complement(418630..421650) Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination YOR049C 0 422668 423732 ON 15 RSB1 complement(422668..423732) Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane YOR050C 0 424270 424617 OFF 15 YOR050C complement(424270..424617) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable YOR051C 0 424846 426084 ON 15 ETT1 complement(424846..426084) Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough YOR052C 0 426772 427224 ON 15 YOR052C complement(426772..427224) Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic YOR053W 1 427817 428158 OFF 15 YOR053W (427817..428158) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C YOR054C 0 427833 429857 ON 15 VHS3 complement(427833..429857) Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis YOR055W 1 429478 429912 OFF 15 YOR055W (429478..429912) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOR056C 0 430247 431626 ON 15 NOB1 complement(430247..431626) Essential nuclear protein involved in proteasome maturation and synthesis of 40S ribosomal subunits; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA YOR057W 1 432186 433373 ON 15 SGT1 (432186..433373) Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes YOR058C 0 433688 436345 ON 15 ASE1 complement(433688..436345) Mitotic spindle midzone localized microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate Scer_15.trna11K 1 438643 438738 ON 15 tK-UUU (438643..438679,438703..438738) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YOR059C 0 438906 440258 ON 15 YOR059C complement(438906..440258) Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor RPN4 YOR060C 0 440390 441163 ON 15 SLD7 complement(440390..441163) Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria YOR061W 1 441534 442553 ON 15 CKA2 (441534..442553) Alpha' catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase YOR062C 0 442725 443531 ON 15 YOR062C complement(442725..443531) Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YOR063W 1 444686 445849 ON 15 RPL3 (444686..445849) Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribosomal proteins; involved in the replication and maintenance of killer double stranded RNA virus YOR064C 0 446079 446738 ON 15 YNG1 complement(446079..446738) Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 YOR065W 1 447439 448368 ON 15 CYT1 (447439..448368) Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex YOR066W 1 449436 451325 ON 15 MSA1 (449436..451325) Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation YOR067C 0 451729 453462 ON 15 ALG8 complement(451729..453462) Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p YOR069W 1 453768 455795 ON 15 VPS5 (453768..455795) Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p YOR068C 0 453869 454213 OFF 15 VAM10 complement(453869..454213) Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering YOR070C 0 455907 457820 ON 15 GYP1 complement(455907..457820) Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion YOR071C 0 459480 461276 ON 15 NRT1 complement(459480..461276) High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity YOR072W 1 461502 461816 OFF 15 YOR072W (461502..461816) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive YOR072W-A 1 461791 462039 OFF 15 YOR072W-A (461791..462039) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR072W; originally identified by fungal homology and RT-PCR Scer_15.trna12P 1 464450 464551 ON 15 tP-UGG (464450..464485,464516..464551) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 59.59 YOR072W-B 1 464469 464630 OFF 15 YOR072W-B (464469..464630) Putative protein of unknown function; identified by expression profiling and mass spectrometry YOR073W 1 464771 466543 ON 15 SGO1 (464771..466543) Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability YOR074C 0 466675 467589 ON 15 CDC21 complement(466675..467589) Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S YOR073W-A 1 467390 467623 OFF 15 YOR073W-A (467390..467623) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CDC21/YOR074C; identified by RT-PCR YOR075W 1 468212 469252 ON 15 UFE1 (468212..469252) t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER YOR076C 0 469377 471620 ON 15 SKI7 complement(469377..471620) Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype YOR077W 1 471899 472597 ON 15 RTS2 (471899..472597) Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication YOR078W 1 472725 473369 ON 15 BUD21 (472725..473369) Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern YOR079C 0 473475 474416 ON 15 ATX2 complement(473475..474416) Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation YOR080W 1 474594 476792 ON 15 DIA2 (474594..476792) Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth YOR081C 0 476939 479188 ON 15 TGL5 complement(476939..479188) Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate YOR082C 0 479296 479637 OFF 15 YOR082C complement(479296..479637) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W YOR083W 1 479533 480420 ON 15 WHI5 (479533..480420) Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 YOR084W 1 480587 481750 ON 15 LPX1 (480587..481750) Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction YOR085W 1 482033 483085 ON 15 OST3 (482033..483085) Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins YOR086C 0 483219 486779 ON 15 TCB1 complement(483219..486779) Lipid-binding ER protein, enriched at ER-plasma membrane contact sites; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Scer_15.trna13N 1 487439 487512 ON 15 tN-GUU (487439..487512) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YOR087W 1 487707 489734 ON 15 YVC1 (487707..489734) Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock YOR089C 0 490196 490828 ON 15 VPS21 complement(490196..490828) Rab family GTPase required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog YOR090C 0 491123 492841 ON 15 PTC5 complement(491123..492841) Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p YOR091W 1 493433 494470 ON 15 TMA46 (493433..494470) Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p YOR092W 1 495127 496968 ON 15 ECM3 (495127..496968) Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation YOR093C 0 497506 502452 ON 15 YOR093C complement(497506..502452) Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents YOR094W 1 502795 503346 ON 15 ARF3 (502795..503346) Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity YOR095C 0 503552 504328 ON 15 RKI1 complement(503552..504328) Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis YOR096W 1 505794 506767 ON 15 RPS7A (505794..505937,506339..506767) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins YOR097C 0 506978 507505 ON 15 YOR097C complement(506978..507505) Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene YOR098C 0 507948 511178 ON 15 NUP1 complement(507948..511178) FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 YOR099W 1 511825 513006 ON 15 KTR1 (511825..513006) Alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family YOR100C 0 513295 514278 ON 15 CRC1 complement(513295..514278) Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation YOR101W 1 515244 516173 ON 15 RAS1 (515244..516173) GTPase involved in G-protein signaling in the adenylate cyclase activating pathway, plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes YOR102W 1 516423 516773 OFF 15 YOR102W (516423..516773) Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex YOR103C 0 516449 516841 ON 15 OST2 complement(516449..516841) Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins YOR104W 1 517642 518490 ON 15 PIN2 (517642..518490) Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated YOR105W 1 518195 518521 OFF 15 YOR105W (518195..518521) Protein of unknown function; expressed at both mRNA and protein levels YOR106W 1 519121 519972 ON 15 VAM3 (519121..519972) Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region YOR107W 1 521353 522282 ON 15 RGS2 (521353..522282) Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p YOR108W 1 523027 524841 ON 15 LEU9 (523027..524841) Alpha-isopropylmalate synthase II (2-isopropylmalate synthase), catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant YOR108C-A 0 524605 524814 OFF 15 YOR108C-A complement(524605..524814) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR109W 1 525278 528601 ON 15 INP53 (525278..528601) Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains YOR110W 1 528941 530248 ON 15 TFC7 (528941..530248) One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes YOR111W 1 530429 531127 ON 15 YOR111W (530429..531127) Putative protein of unknown function YOR112W 1 531508 533793 ON 15 CEX1 (531508..533793) Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p YOR113W 1 534075 536819 ON 15 AZF1 (534075..536819) Zinc-finger transcription factor, involved in induction of CLN3 transcription in response to glucose; genetic and physical interactions indicate a possible role in mitochondrial transcription or genome maintenance YOR114W 1 537570 538454 ON 15 YOR114W (537570..538454) Putative protein of unknown function; null mutant is viable YOR115C 0 538659 539465 ON 15 TRS33 complement(538659..539465) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic YOR116C 0 539763 544145 ON 15 RPO31 complement(539763..544145) RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 YOR117W 1 545029 546333 ON 15 RPT5 (545029..546333) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 YOR118W 1 546857 548560 ON 15 RTC5 (546857..548560) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity YOR119C 0 548792 550246 ON 15 RIO1 complement(548792..550246) Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA YOR120W 1 551114 552052 ON 15 GCY1 (551114..552052) Putative NADP(+) coupled glycerol dehydrogenase, proposed to be involved in an alternative pathway for glycerol catabolism; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family YOR121C 0 551798 552103 OFF 15 YOR121C complement(551798..552103) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W YOR122C 0 552298 552887 ON 15 PFY1 complement(552298..552665,552875..552887) Profilin, binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress YOR123C 0 553176 554570 ON 15 LEO1 complement(553176..554570) Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay YOR124C 0 554824 558642 ON 15 UBP2 complement(554824..558642) Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity YOR125C 0 559030 559731 ON 15 CAT5 complement(559030..559731) Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation YOR126C 0 559961 560677 ON 15 IAH1 complement(559961..560677) Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing YOR127W 1 561170 564193 ON 15 RGA1 (561170..564193) GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth YOR128C 0 564476 566191 ON 15 ADE2 complement(564476..566191) Phosphoribosylaminoimidazole carboxylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine YOR129C 0 566877 569558 ON 15 AFI1 complement(566877..569558) Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p YOR130C 0 569929 570807 ON 15 ORT1 complement(569929..570807) Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Scer_15.trna14D 1 571958 572029 ON 15 tD-GUC (571958..572029) Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis; tRNA Type: Asp (D) | Anti-Codon: GUC | Cove Score: 58.36 YOR131C 0 572182 572838 ON 15 YOR131C complement(572182..572838) Putative protein of unknown function; non-essential gene; overexpression causes a cell cycle delay or arrest YOR132W 1 573175 574830 ON 15 VPS17 (573175..574830) Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation YOR133W 1 575098 577626 ON 15 EFT1 (575098..577626) Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin YOR134W 1 578564 579793 ON 15 BAG7 (578564..579793) Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p YOR135C 0 580159 580500 OFF 15 IRC14 complement(580159..580500) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci YOR136W 1 580250 581359 ON 15 IDH2 (580250..581359) Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated YOR137C 0 581813 583681 ON 15 SIA1 complement(581813..583681) Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose YOR138C 0 584309 586324 ON 15 RUP1 complement(584309..586324) Protein involved in regulation of Rsp5p, which is an essential HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain YOR139C 0 586950 587342 OFF 15 YOR139C complement(586950..587342) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W YOR140W 1 586981 589281 ON 15 SFL1 (586981..589281) Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p YOR141C 0 589942 592587 ON 15 ARP8 complement(589942..592587) Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity YOR142W 1 593057 594046 ON 15 LSC1 (593057..594046) Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Scer_15.trna15G 0 594354 594425 ON 15 tG-CCC complement(594354..594425) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons; tRNA Type: Gly (G) | Anti-Codon: CCC | Cove Score: 80.95 YOR143C 0 601383 602342 ON 15 THI80 complement(601383..602342) Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) YOR144C 0 602717 605092 ON 15 ELG1 complement(602717..605092) Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis YOR145C 0 605347 606171 ON 15 PNO1 complement(605347..606171) Essential nucleolar protein required for pre-18S rRNA processing, interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain YOR146W 1 605873 606178 OFF 15 YOR146W (605873..606178) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C YOR147W 1 606607 608475 ON 15 MDM32 (606607..608475) Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 YOR148C 0 608640 609197 ON 15 SPP2 complement(608640..609197) Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction YOR149C 0 609838 611388 ON 15 SMP3 complement(609838..611388) Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure YOR150W 1 611999 612490 ON 15 MRPL23 (611999..612490) Mitochondrial ribosomal protein of the large subunit YOR151C 0 612997 616671 ON 15 RPB2 complement(612997..616671) RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit YOR152C 0 617518 618288 ON 15 YOR152C complement(617518..618288) Putative protein of unknown function; YOR152C is not an essential gene YOR153W 1 619840 624375 ON 15 PDR5 (619840..624375) Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth YOR154W 1 624729 626492 ON 15 SLP1 (624729..626492) Integral membrane protein of unknown function; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins YOR155C 0 626628 627980 ON 15 ISN1 complement(626628..627980) Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms YOR156C 0 628360 630540 ON 15 NFI1 complement(628360..630540) SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length YOR157C 0 630966 631751 ON 15 PUP1 complement(630966..631751) Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z YOR158W 1 632164 633120 ON 15 PET123 (632164..633120) Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator YOR159C 0 633282 633566 ON 15 SME1 complement(633282..633566) Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E YOR160W 1 633839 636757 ON 15 MTR10 (633839..636757) Nuclear import receptor, mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs YOR161C 0 636939 638558 ON 15 PNS1 complement(636939..638558) Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport YOR161W-A 1 637574 637654 OFF 15 YOR161W-A (637574..637654) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR161W-B 1 637975 638235 OFF 15 YOR161W-B (637975..638235) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR161C-C 0 639121 639267 OFF 15 YOR161C-C complement(639121..639267) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR162C 0 639560 641992 ON 15 YRR1 complement(639560..641992) Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes YOR163W 1 642741 643307 ON 15 DDP1 (642741..643307) Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; has high specificity for diadenosine hexa- and pentaphosphates; exhibits endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; member of the MutT family of nucleotide hydrolases YOR164C 0 643394 644332 ON 15 GET4 complement(643394..644332) Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 YOR165W 1 644566 646896 ON 15 SEY1 (644566..646896) Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 YOR166C 0 647126 648502 ON 15 SWT1 complement(647126..648502) RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain YOR167C 0 648804 649007 ON 15 RPS28A complement(648804..649007) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Bp and has similarity to rat S28 ribosomal protein YOR168W 1 649303 651732 ON 15 GLN4 (649303..651732) Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions YOR169C 0 651376 651840 OFF 15 YOR169C complement(651376..651840) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W YOR170W 1 651858 652163 OFF 15 YOR170W (651858..652163) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 YOR171C 0 652010 653884 ON 15 LCB4 complement(652010..653884) Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes YOR172W 1 654210 656570 ON 15 YRM1 (654210..656570) Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes YOR173W 1 657264 658325 ON 15 DCS2 (657264..658325) Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance YOR174W 1 658747 659601 ON 15 MED4 (658747..659601) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YOR175C 0 659815 661674 ON 15 ALE1 complement(659815..661674) Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids YOR176W 1 662401 663582 ON 15 HEM15 (662401..663582) Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Scer_15.trna16V 0 663812 663885 ON 15 tV-AAC complement(663812..663885) Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis; tRNA Type: Val (V) | Anti-Codon: AAC | Cove Score: 67.95 YOR177C 0 665785 667179 ON 15 MPC54 complement(665785..667179) Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate YOR178C 0 667860 670241 ON 15 GAC1 complement(667860..670241) Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock YOR179C 0 671845 672411 ON 15 SYC1 complement(671845..672411) Subunit of the APT subcomplex of cleavage and polyadenylation factor, may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs YOR180C 0 674352 675167 ON 15 DCI1 complement(674352..675167) Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed YOR181W 1 675939 677840 ON 15 LAS17 (675939..677840) Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) YOR182C 0 678191 678793 ON 15 RPS30B complement(678191..678379,678791..678793) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Ap and has similarity to rat S30 ribosomal protein YOR183W 1 678872 679261 OFF 15 FYV12 (678872..679261) Protein of unknown function, required for survival upon exposure to K1 killer toxin YOR184W 1 679357 680544 ON 15 SER1 (679357..680544) 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p Scer_snR36 0 680685 680866 ON 15 SNR36 complement(680685..680866) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at position U1187 YOR185C 0 681444 682106 ON 15 GSP2 complement(681444..682106) GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability YOR186W 1 683111 683545 ON 15 YOR186W (683111..683545) Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent YOR186C-A 0 683329 683538 OFF 15 YOR186C-A complement(683329..683538) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR187W 1 684030 685343 ON 15 TUF1 (684030..685343) Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans YOR188W 1 685767 689180 ON 15 MSB1 (685767..689180) Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4 YOR189W 1 689624 689974 ON 15 IES4 (689624..689974) Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses YOR190W 1 690695 692032 ON 15 SPR1 (690695..692032) Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance YOR191W 1 692475 697334 ON 15 ULS1 (692475..697334) RING finger protein involved in proteolytic control of sumoylated substrates; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching YOR192C 0 698768 700567 ON 15 THI72 complement(698768..700567) Transporter of thiamine or related compound; shares sequence similarity with Thi7p YOR192C-C 0 703988 704224 OFF 15 YOR192C-C complement(703988..704224) Putative protein of unknown function; identified by expression profiling and mass spectrometry Scer_15.trna17M 1 710201 710272 ON 15 tM-CAU (710201..710272) Methionine initiator tRNA (tRNA-Met), predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 74.96 YOR193W 1 710446 711576 ON 15 PEX27 (710446..711576) Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p YOR194C 0 711683 712543 ON 15 TOA1 complement(711683..712543) TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA YOR195W 1 712866 715331 ON 15 SLK19 (712866..715331) Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate YOR196C 0 715593 716837 ON 15 LIP5 complement(715593..716837) Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase YOR197W 1 717086 718384 ON 15 MCA1 (717086..718384) Ca2+-activated cysteine protease; may cleave specific substrates during stress response; involved in regulation of apoptosis upon H2O2 treatment; contributes to clearance of insoluble protein aggregates during normal growth; may be involved in cell cycle progression; similar to mammalian caspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet with the 6 caspase-common beta-strands, blocking potential dimerization YOR198C 0 718653 720065 ON 15 BFR1 complement(718653..720065) Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity YOR199W 1 720181 720510 OFF 15 YOR199W (720181..720510) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOR200W 1 720417 720815 OFF 15 YOR200W (720417..720815) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c YOR201C 0 720470 721708 ON 15 MRM1 complement(720470..721708) Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA YOR202W 1 721946 722608 ON 15 HIS3 (721946..722608) Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p YOR203W 1 722565 722918 OFF 15 YOR203W (722565..722918) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation YOR204W 1 722911 724725 ON 15 DED1 (722911..724725) ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility YOR205C 0 725564 727234 ON 15 GEP3 complement(725564..727234) Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) YOR206W 1 727512 729644 ON 15 NOC2 (727512..729644) Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors YOR207C 0 730008 733457 ON 15 RET1 complement(730008..733457) Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs YOR208W 1 733925 736177 ON 15 PTP2 (733925..736177) Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus YOR209C 0 736437 737726 ON 15 NPT1 complement(736437..737726) Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus YOR210W 1 738320 738532 ON 15 RPB10 (738320..738532) RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III YOR211C 0 738924 741569 ON 15 MGM1 complement(738924..741569) Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy YOR212W 1 742910 744181 ON 15 STE4 (742910..744181) G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats YOR213C 0 744535 745281 ON 15 SAS5 complement(744535..745281) Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity YOR214C 0 745593 746303 ON 15 YOR214C complement(745593..746303) Putative protein of unknown function; YOR214C is not an essential gene YOR215C 0 746725 747282 ON 15 AIM41 complement(746725..747282) Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss YOR216C 0 747525 748979 ON 15 RUD3 complement(747525..748979) Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 YOR217W 1 749301 751886 ON 15 RFC1 (749301..751886) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YOR218C 0 751476 751895 OFF 15 YOR218C complement(751476..751895) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W YOR219C 0 752214 755009 ON 15 STE13 complement(752214..755009) Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor YOR220W 1 755328 756125 ON 15 RCN2 (755328..756125) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; phosphorylated in response to alpha factor YOR221C 0 756476 757558 ON 15 MCT1 complement(756476..757558) Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling YOR222W 1 758330 759253 ON 15 ODC2 (758330..759253) Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Scer_snR35 0 759326 759529 ON 15 SNR35 complement(759326..759529) H/ACA box small nucleolar RNA (snoRNA); predicted to guide pseudouridylation of small subunit (SSU) rRNA at position 1191 YOR223W 1 759782 760660 ON 15 YOR223W (759782..760660) Protein of unknown function found in the ER and vacuole lumen; overexpression of YOR223W affects endocytic protein trafficking YOR224C 0 760825 761265 ON 15 RPB8 complement(760825..761265) RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III YOR225W 1 761392 761721 OFF 15 YOR225W (761392..761721) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOR226C 0 761614 762084 ON 15 ISU2 complement(761614..762084) Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable YOR227W 1 762825 766565 ON 15 HER1 (762825..766565) Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments YOR228C 0 766869 767777 ON 15 YOR228C complement(766869..767777) Protein of unknown function, localized to the mitochondrial outer membrane YOR229W 1 768409 769812 ON 15 WTM2 (768409..769812) Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats YOR230W 1 770800 772113 ON 15 WTM1 (770800..772113) Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats YOR231W 1 772601 774127 ON 15 MKK1 (772601..774127) Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p YOR231C-A 0 773163 773363 OFF 15 YOR231C-A complement(773163..773363) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR232W 1 774573 775259 ON 15 MGE1 (774573..775259) Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE YOR233W 1 775846 778248 ON 15 KIN4 (775846..778248) Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck YOR234C 0 778555 779405 ON 15 RPL33B complement(778555..778859,779387..779405) Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has similarity to rat L35a; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable YOR235W 1 779870 780184 OFF 15 IRC13 (779870..780184) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Scer_snR17a 1 780107 780596 ON 15 SNR17A (780107..780120,780278..780596) small nucleolar RNA (snoRNA) U3, part of Small ribosomal SubUnit (SSU) processosome; SNR17B also encodes snoRNA U3 YOR236W 1 780906 781541 ON 15 DFR1 (780906..781541) Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism YOR237W 1 781994 783298 ON 15 HES1 (781994..783298) Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP) YOR238W 1 783677 784588 ON 15 YOR238W (783677..784588) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YOR239W 1 784857 786744 ON 15 ABP140 (784857..785687,785689..786744) AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift YOR241W 1 786995 788641 ON 15 MET7 (786995..788641) Folylpolyglutamate synthetase, catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA YOR242C 0 788742 789857 ON 15 SSP2 complement(788742..789857) Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis YOR243C 0 790211 792241 ON 15 PUS7 complement(790211..792241) Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria YOR244W 1 792531 793868 ON 15 ESA1 (792531..793868) Catalytic subunit of the histone acetyltransferase complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression and transcriptional silencing at the rDNA locus YOR245C 0 794076 795332 ON 15 DGA1 complement(794076..795332) Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles YOR246C 0 795801 796793 ON 15 ENV9 complement(795801..796793) Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts YOR247W 1 797676 798308 ON 15 SRL1 (797676..798308) Mannoprotein that exhibits a tight association with the cell wall, required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants YOR248W 1 798013 798315 OFF 15 YOR248W (798013..798315) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YOR249C 0 798674 800731 ON 15 APC5 complement(798674..800731) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis YOR250C 0 800970 802307 ON 15 CLP1 complement(800970..802307) Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation YOR251C 0 802550 803464 ON 15 TUM1 complement(802550..803464) Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized YOR252W 1 803667 804203 ON 15 TMA16 (803667..804203) Protein of unknown function that associates with ribosomes YOR253W 1 804377 804907 ON 15 NAT5 (804377..804907) Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing YOR254C 0 805032 807023 ON 15 SEC63 complement(805032..807023) Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YOR255W 1 807271 808107 ON 15 OSW1 (807271..808107) Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p YOR256C 0 808254 810683 ON 15 TRE2 complement(808254..810683) Protein that functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; has similarity to transferrin receptors; inviability of null mutant in systematic studies is due to proximity to CDC31 YOR257W 1 811008 811493 ON 15 CDC31 (811008..811493) Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation YOR258W 1 811671 812324 ON 15 HNT3 (811671..812324) DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia YOR259C 0 812395 813708 ON 15 RPT4 complement(812395..813708) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization YOR260W 1 813984 815720 ON 15 GCD1 (813984..815720) Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YOR261C 0 815915 816931 ON 15 RPN8 complement(815915..816931) Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p YOR262W 1 817292 818335 ON 15 YOR262W (817292..818335) Protein of unknown function required for establishment of sister chromatid cohesion; contains an ATP/GTP binding site motif; similar to YLR243W and is highly conserved across species and homologous to human gene GPN2/ATPBD1B YOR263C 0 818757 819164 OFF 15 YOR263C complement(818757..819164) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W YOR264W 1 818866 820158 ON 15 DSE3 (818866..820158) Daughter cell-specific protein, may help establish daughter fate YOR265W 1 820454 820774 ON 15 RBL2 (820454..820774) Protein involved in microtubule morphogenesis, required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A YOR266W 1 821023 822294 ON 15 PNT1 (821023..822294) Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine YOR267C 0 822588 824867 ON 15 HRK1 complement(822588..824867) Protein kinase implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis YOR268C 0 825534 825932 OFF 15 YOR268C complement(825534..825932) Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene YOR269W 1 826385 827869 ON 15 PAC1 (826385..827869) Protein involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; synthetic lethal with bni1; homolog of human LIS1 YOR270C 0 828052 830574 ON 15 VPH1 complement(828052..830574) Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes YOR271C 0 831059 832042 ON 15 FSF1 complement(831059..832042) Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Scer_snR8 1 832332 832521 ON 15 SNR8 (832332..832521) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U960 and U986 YOR272W 1 832813 834195 ON 15 YTM1 (832813..834195) Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats YOR273C 0 834452 836431 ON 15 TPO4 complement(834452..836431) Polyamine transport protein, recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane; member of the major facilitator superfamily YOR274W 1 837674 838960 ON 15 MOD5 (837674..838960) Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis YOR275C 0 839084 841069 ON 15 RIM20 complement(839084..841069) Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation YOR276W 1 841333 841818 ON 15 CAF20 (841333..841818) Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E YOR277C 0 841515 841823 OFF 15 YOR277C complement(841515..841823) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Scer_snR31 0 841958 842182 ON 15 SNR31 complement(841958..842182) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at position U999 Scer_snR5 1 842403 842606 ON 15 SNR5 (842403..842606) H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U1004 and U1124 YOR278W 1 842817 843644 ON 15 HEM4 (842817..843644) Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria YOR279C 0 843698 844630 ON 15 RFM1 complement(843698..844630) DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance YOR280C 0 844992 845792 ON 15 FSH3 complement(844992..845792) Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 YOR281C 0 846269 847129 ON 15 PLP2 complement(846269..847129) Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP YOR282W 1 847000 847320 OFF 15 YOR282W (847000..847320) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C YOR283W 1 847453 848145 ON 15 YOR283W (847453..848145) Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene YOR284W 1 848478 849209 ON 15 HUA2 (848478..849209) Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly YOR285W 1 849635 850054 ON 15 RDL1 (849635..850054) Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane YOR286W 1 850280 850729 ON 15 RDL2 (850280..850729) Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss YOR287C 0 850937 851839 ON 15 RRP36 complement(850937..851839) Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit YOR288C 0 852121 853077 ON 15 MPD1 complement(852121..853077) Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation YOR289W 1 853357 854112 ON 15 YOR289W (853357..854112) Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Scer_15.trna18A 0 854187 854259 ON 15 tA-UGC complement(854187..854259) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: UGC | Cove Score: 76.57 YOR290C 0 855147 860258 ON 15 SNF2 complement(855147..860258) Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p YOR291W 1 861175 865593 ON 15 YPK9 (861175..865593) Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome YOR292C 0 865653 866582 ON 15 YOR292C complement(865653..866582) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene YOR293W 1 867098 867852 ON 15 RPS10A (867098..867149,867587..867852) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps10Bp and has similarity to rat ribosomal protein S10 YOR293C-A 0 867998 868147 OFF 15 YOR293C-A complement(867998..868147) Putative protein of unknown function; identified by expression profiling and mass spectrometry YOR294W 1 868340 868951 ON 15 RRS1 (868340..868951) Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice YOR295W 1 869208 869894 ON 15 UAF30 (869208..869894) Subunit of UAF (upstream activation factor), which is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate YOR296W 1 870202 874071 ON 15 YOR296W (870202..874071) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene YOR297C 0 874743 875321 ON 15 TIM18 complement(874743..875321) Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex YOR298W 1 875599 877038 ON 15 MUM3 (875599..877038) Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases YOR298C-A 0 877230 877685 ON 15 MBF1 complement(877230..877685) Transcriptional coactivator that bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations YOR299W 1 878435 880675 ON 15 BUD7 (878435..880675) Member of the ChAPs family of proteins (Chs5p-Arf1p-binding proteins: Bch1p, Bch2p, Bud7p, Chs6p), that forms the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane YOR300W 1 880575 880883 OFF 15 YOR300W (880575..880883) Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 YOR301W 1 880965 882272 ON 15 RAX1 (880965..882272) Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily YOR302W 1 882765 882842 ON 15 YOR302W (882765..882842) CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA YOR303W 1 882899 884134 ON 15 CPA1 (882899..884134) Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader YOR304W 1 884514 887876 ON 15 ISW2 (884514..887876) ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth YOR304C-A 0 888520 888750 ON 15 YOR304C-A complement(888520..888750) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck YOR305W 1 889022 889750 ON 15 RRG7 (889022..889750) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene YOR306C 0 889867 891432 ON 15 MCH5 complement(889867..891432) Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport YOR307C 0 892731 894092 ON 15 SLY41 complement(892731..894092) Protein involved in ER-to-Golgi transport YOR308C 0 894624 896387 ON 15 SNU66 complement(894624..896387) Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 YOR309C 0 896698 897078 OFF 15 YOR309C complement(896698..897078) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 YOR310C 0 896825 898360 ON 15 NOP58 complement(896825..898360) Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA YOR311C 0 899056 899928 ON 15 DGK1 complement(899056..899928) Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain YOR312C 0 900250 901194 ON 15 RPL20B complement(900250..900767,901194..901194) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similarity to rat L18a ribosomal protein YOR313C 0 901858 902874 ON 15 SPS4 complement(901858..902874) Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage YOR314W 1 903043 903372 OFF 15 YOR314W (903043..903372) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOR314W-A 1 904458 904568 OFF 15 YOR314W-A (904458..904568) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YOR315W 1 904760 905800 ON 15 SFG1 (904760..905800) Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate YOR316C 0 906236 907555 ON 15 COT1 complement(906236..907555) Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium YOR316C-A 0 907726 907935 OFF 15 YOR316C-A complement(907726..907935) Putative protein of unknown function; identified by fungal homology and RT-PCR YOR317W 1 909343 911445 ON 15 FAA1 (909343..911445) Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism YOR318C 0 911784 912436 OFF 15 YOR318C complement(911784..912085,912433..912436) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo YOR319W 1 912822 913463 ON 15 HSH49 (912822..913463) U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) YOR320C 0 913619 915094 ON 15 GNT1 complement(913619..915094) N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus YOR321W 1 916030 918291 ON 15 PMT3 (916030..918291) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; target for new antifungals YOR322C 0 918606 921062 ON 15 LDB19 complement(918606..921062) Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye YOR323C 0 921535 922905 ON 15 PRO2 complement(921535..922905) Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis YOR324C 0 923232 925040 ON 15 FRT1 complement(923232..925040) Tail-anchored ER membrane protein that is a substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation YOR325W 1 924577 925050 OFF 15 YOR325W (924577..925050) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 YOR326W 1 925721 930445 ON 15 MYO2 (925721..930445) One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle YOR327C 0 930734 931081 ON 15 SNC2 complement(930734..931081) Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins YOR328W 1 931803 936497 ON 15 PDR10 (931803..936497) ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p YOR329C 0 936731 939349 ON 15 SCD5 complement(936731..939349) Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency YOR329W-A 1 939348 939557 OFF 15 YOR329W-A (939348..939557) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YOR330C 0 939621 943385 ON 15 MIP1 complement(939621..943385) Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit YOR331C 0 943565 944122 OFF 15 YOR331C complement(943565..944122) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W YOR332W 1 943656 944357 ON 15 VMA4 (943656..944357) Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane YOR333C 0 944541 944957 OFF 15 YOR333C complement(944541..944957) Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth YOR334W 1 944596 946008 ON 15 MRS2 (944596..946008) Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns YOR335C 0 946233 949109 ON 15 ALA1 complement(946233..949109) Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog YOR335W-A 1 946568 946648 OFF 15 YOR335W-A (946568..946648) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ALA1/YOR335C YOR336W 1 949773 953870 ON 15 KRE5 (949773..953870) Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects YOR337W 1 954344 956623 ON 15 TEA1 (954344..956623) Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein YOR338W 1 956898 957989 ON 15 YOR338W (956898..957989) Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen YOR339C 0 958362 958832 ON 15 UBC11 complement(958362..958832) Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 YOR340C 0 959202 960182 ON 15 RPA43 complement(959202..960182) RNA polymerase I subunit A43 YOR341W 1 960987 965981 ON 15 RPA190 (960987..965981) RNA polymerase I largest subunit A190 YOR342C 0 966666 967625 ON 15 YOR342C complement(966666..967625) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus YOR343C 0 968148 968474 OFF 15 YOR343C complement(968148..968474) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_15.trna19M 1 976421 976493 ON 15 tM-CAU (976421..976493) Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 70.03 YOR344C 0 977194 978069 ON 15 TYE7 complement(977194..978069) Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Scer_15.trna20P 1 980683 980787 ON 15 tP-UGG (980683..980718,980752..980787) Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; tRNA Type: Pro (P) | Anti-Codon: UGG | Cove Score: 55.42 YOR345C 0 981812 982162 OFF 15 YOR345C complement(981812..982162) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 YOR346W 1 981828 984785 ON 15 REV1 (981828..984785) Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; YOR347C 0 984942 986462 ON 15 PYK2 complement(984942..986462) Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by glucose, and Pyk2p may be active under low glycolytic flux YOR348C 0 986899 988782 ON 15 PUT4 complement(986899..988782) Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells YOR349W 1 989789 992833 ON 15 CIN1 (989789..992833) Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl YOR350C 0 992864 994855 ON 15 MNE1 complement(992864..994855) Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA YOR351C 0 995018 996511 ON 15 MEK1 complement(995018..996511) Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids YOR352W 1 997213 998244 ON 15 TFB6 (997213..998244) Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YOR353C 0 998453 1000828 ON 15 SOG2 complement(998453..1000828) Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis YOR354C 0 1001147 1003225 ON 15 MSC6 complement(1001147..1003225) Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOR355W 1 1005137 1006705 ON 15 GDS1 (1005137..1006705) Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOR356W 1 1007221 1009116 ON 15 CIR2 (1007221..1009116) Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response YOR357C 0 1009224 1009712 ON 15 SNX3 complement(1009224..1009712) Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p YOR358W 1 1010161 1010889 ON 15 HAP5 (1010161..1010889) Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex YOR359W 1 1011189 1012760 ON 15 VTS1 (1011189..1012760) Post-transcriptional gene regulator, flap-structured DNA-binding and RNA-binding protein; shows genetic interactions with Vti1p, a v-SNARE involved in cis-Golgi membrane traffic; stimulates Dna2p endonuclease activity; contains a SAM domain YOR360C 0 1013241 1014821 ON 15 PDE2 complement(1013241..1014821) High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon YOR361C 0 1015361 1017652 ON 15 PRT1 complement(1015361..1017652) eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes YOR362C 0 1017880 1018746 ON 15 PRE10 complement(1017880..1018746) Alpha 7 subunit of the 20S proteasome YOR363C 0 1020222 1023212 ON 15 PIP2 complement(1020222..1023212) Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes YOR364W 1 1023371 1023739 OFF 15 YOR364W (1023371..1023739) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C YOR365C 0 1023461 1025572 ON 15 YOR365C complement(1023461..1025572) Putative protein of unknown function; YOR365C is not an essential protein YOR366W 1 1025256 1025609 OFF 15 YOR366W (1025256..1025609) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C YOR367W 1 1026007 1026609 ON 15 SCP1 (1026007..1026609) Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin YOR368W 1 1026843 1028048 ON 15 RAD17 (1026843..1028048) Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins YOR369C 0 1028194 1028625 ON 15 RPS12 complement(1028194..1028625) Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 YOR370C 0 1029183 1030994 ON 15 MRS6 complement(1029183..1030994) Rab escort protein, forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene YOR371C 0 1031489 1034182 ON 15 GPB1 complement(1031489..1034182) Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; homolog of Gpb2p YOR372C 0 1034807 1036471 ON 15 NDD1 complement(1034807..1036471) Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes YOR373W 1 1036834 1039389 ON 15 NUD1 (1036834..1039389) Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance YOR374W 1 1039840 1041399 ON 15 ALD4 (1039840..1041399) Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed YOR375C 0 1041678 1043042 ON 15 GDH1 complement(1041678..1043042) NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources YOR376W 1 1043191 1043559 OFF 15 YOR376W (1043191..1043559) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. YOR376W-A 1 1045196 1045351 OFF 15 YOR376W-A (1045196..1045351) Putative protein of unknown function; identified by fungal homology and RT-PCR YOR377W 1 1046226 1047803 ON 15 ATF1 (1046226..1047803) Alcohol acetyltransferase with potential roles in lipid and sterol metabolism; responsible for the major part of volatile acetate ester production during fermentation YOR378W 1 1049511 1051058 ON 15 YOR378W (1049511..1051058) Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene YOR379C 0 1050726 1051064 OFF 15 YOR379C complement(1050726..1051064) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W YOR380W 1 1051290 1052930 ON 15 RDR1 (1051290..1052930) Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins YOR381W 1 1055545 1057680 ON 15 FRE3 (1055545..1057680) Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels YOR381W-A 1 1058423 1058590 OFF 15 YOR381W-A (1058423..1058590) Putative protein of unknown function; identified by fungal homology and RT-PCR YOR382W 1 1059531 1059992 ON 15 FIT2 (1059531..1059992) Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall YOR383C 0 1060441 1061055 ON 15 FIT3 complement(1060441..1061055) Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall YOR384W 1 1061564 1063648 ON 15 FRE5 (1061564..1063648) Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YOR385W 1 1065043 1065915 ON 15 YOR385W (1065043..1065915) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene YOR386W 1 1066839 1068536 ON 15 PHR1 (1066839..1068536) DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p YOR387C 0 1069621 1070241 ON 15 YOR387C complement(1069621..1070241) Putative protein of unknown function; regulated by the metal-responsive Aft1p transcription factor; highly inducible in zinc-depleted conditions; localizes to the soluble fraction YOR388C 0 1071793 1072923 ON 15 FDH1 complement(1071793..1072923) NAD(+)-dependent formate dehydrogenase, may protect cells from exogenous formate YOR389W 1 1074213 1076087 ON 15 YOR389W (1074213..1076087) Putative protein of unknown function; expression regulated by copper levels YOR390W 1 1076784 1077911 ON 15 YOR390W (1076784..1077911) Putative protein of unknown function YOR391C 0 1078545 1079258 ON 15 HSP33 complement(1078545..1079258) Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease YOR392W 1 1079282 1079725 OFF 15 YOR392W (1079282..1079725) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; gene expression induced by heat YOR393W 1 1080276 1081589 ON 15 ERR1 (1080276..1081589) Protein of unknown function, has similarity to enolases YOR394W 1 1082718 1083212 ON 15 PAU21 (1082718..1083212) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau22p; encodes 2 proteins that are translated from 2 different start codons YOR394C-A 0 1084202 1084369 OFF 15 YOR394C-A complement(1084202..1084369) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YOR396W 1 1085473 1090863 ON 15 YRF1-8 (1085473..1090863) One of several telomeric Y' element-encoded DNA helicases, known as Y'-Help1 (Y'-HELicase Protein 1) YOR396C-A 0 1090015 1090497 OFF 15 YOR396C-A complement(1090015..1090497) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL283C 0 280 6007 ON 16 YRF1-7 complement(280..5840,5989..6007) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YPL283W-B 1 643 1125 OFF 16 YPL283W-B (643..1125) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL283W-A 1 1426 2001 OFF 16 YPL283W-A (1426..2001) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL282C 0 7933 8427 ON 16 PAU22 complement(7933..8427) Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau21p; encodes 2 proteins that are translated from 2 different start codons YPL281C 0 9557 10870 ON 16 ERR2 complement(9557..10870) Protein of unknown function, has similarity to enolases YPL280W 1 11887 12600 ON 16 HSP32 (11887..12600) Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease YPL279C 0 13228 14355 ON 16 YPL279C complement(13228..14355) Putative protein of unknown function YPL278C 0 15053 15355 ON 16 YPL278C complement(15053..15355) Putative protein of unknown function; gene expression regulated by copper levels YPL277C 0 15405 16868 ON 16 YPL277C complement(15405..16868) Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels YPL276W 1 17948 18385 ON 16 YPL276W (17948..18385) NAD(+)-dependent formate dehydrogenase, may protect cells from exogenous formate; YPL275W and YPL276W comprise a continuous open reading frame in some S. cerevisiae strains but not in the genomic reference strain S288C YPL275W 1 18369 19079 ON 16 FDH2 (18369..19079) NAD(+)-dependent formate dehydrogenase, may protect cells from exogenous formate; YPL275W and YPL276W comprise a continuous open reading frame in some S. cerevisiae strains but not in the genomic reference strain S288C YPL274W 1 22938 24701 ON 16 SAM3 (22938..24701) High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p YPL273W 1 25087 26064 ON 16 SAM4 (25087..26064) S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio YPL272C 0 26611 28164 ON 16 YPL272C complement(26611..28164) Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene YPL271W 1 30079 30267 ON 16 ATP15 (30079..30267) Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated YPL270W 1 30482 32803 ON 16 MDL2 (30482..32803) Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis YPL269W 1 33013 34947 ON 16 KAR9 (33013..34947) Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase YPL268W 1 35236 37845 ON 16 PLC1 (35236..37845) Phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes YPL267W 1 38169 38798 ON 16 ACM1 (38169..38798) Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein YPL266W 1 39121 40077 ON 16 DIM1 (39121..40077) Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing YPL265W 1 41043 42869 ON 16 DIP5 (41043..42869) Dicarboxylic amino acid permease, mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly YPL264C 0 43283 44344 ON 16 YPL264C complement(43283..44344) Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene YPL263C 0 44551 46506 ON 16 KEL3 complement(44551..46506) Cytoplasmic protein of unknown function YPL262W 1 47336 48802 ON 16 FUM1 (47336..48802) Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria YPL261C 0 48996 49304 OFF 16 YPL261C complement(48996..49304) Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W YPL260W 1 49303 50958 ON 16 YPL260W (49303..50958) Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene YPL259C 0 51244 52671 ON 16 APM1 complement(51244..52671) Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting YPL258C 0 53498 55153 ON 16 THI21 complement(53498..55153) Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Scer_16.trna1W 0 56169 56274 ON 16 tW-CCA complement(56169..56204,56239..56274) Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis; tRNA Type: Trp (W) | Anti-Codon: CCA | Cove Score: 67.27 YPL257W 1 63279 63860 ON 16 YPL257W (63279..63860) Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene YPL256C 0 64977 66614 ON 16 CLN2 complement(64977..66614) G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) YPL255W 1 67725 68882 ON 16 BBP1 (67725..68882) Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p YPL254W 1 69485 70951 ON 16 HFI1 (69485..70951) Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions YPL253C 0 71063 73006 ON 16 VIK1 complement(71063..73006) Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p YPL252C 0 73363 73881 ON 16 YAH1 complement(73363..73881) Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin YPL251W 1 73625 73927 OFF 16 YPL251W (73625..73927) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C YPL250C 0 74309 74719 ON 16 ICY2 complement(74309..74719) Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate YPL249C-A 0 75699 76239 ON 16 RPL36B complement(75699..75985,76224..76239) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Ap and has similarity to rat L36 ribosomal protein; binds to 5.8 S rRNA YPL250W-A 1 75702 75989 OFF 16 YPL250W-A (75702..75989) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified ORF RPL36B/YPL249C-A; identified by fungal homology and RT-PCR YPL249C 0 76669 79353 ON 16 GYP5 complement(76669..79353) GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth YPL248C 0 79711 82356 ON 16 GAL4 complement(79711..82356) DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p YPL247C 0 82625 84196 ON 16 YPL247C complement(82625..84196) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest YPL246C 0 84509 85297 ON 16 RBD2 complement(84509..85297) Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p YPL245W 1 85586 86950 ON 16 YPL245W (85586..86950) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm YPL244C 0 87014 88033 ON 16 HUT1 complement(87014..88033) Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 YPL243W 1 88517 90316 ON 16 SRP68 (88517..90316) Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane YPL242C 0 90622 95109 ON 16 IQG1 complement(90622..95109) Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family YPL241C 0 95372 96258 ON 16 CIN2 complement(95372..96153,96234..96258) GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 YPL240C 0 96496 98625 ON 16 HSP82 complement(96496..98625) Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p YPL239W 1 99484 100086 ON 16 YAR1 (99484..100086) Cytoplasmic ankyrin-repeat containing protein of unknown function, proposed to link the processes of 40S ribosomal subunit biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock YPL238C 0 100474 100863 OFF 16 YPL238C complement(100474..100863) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W YPL237W 1 100496 101353 ON 16 SUI3 (100496..101353) Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding YPL236C 0 101608 102702 ON 16 ENV7 complement(101608..102702) Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; has sequence similarity with the human serine/threonine protein kinase STK16 YPL235W 1 103232 104647 ON 16 RVB2 (103232..104647) ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly YPL234C 0 104946 105440 ON 16 VMA11 complement(104946..105440) Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen YPL233W 1 106172 106822 ON 16 NSL1 (106172..106822) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation YPL232W 1 107275 108147 ON 16 SSO1 (107275..108147) Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p YPL231W 1 108652 114315 ON 16 FAS2 (108652..114315) Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities YPL230W 1 115312 116487 ON 16 USV1 (115312..116487) Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis YPL229W 1 117067 117687 ON 16 YPL229W (117067..117687) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL229W is not an essential gene YPL228W 1 118382 120031 ON 16 CET1 (118382..120031) Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide YPL227C 0 120163 121167 ON 16 ALG5 complement(120163..121167) UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum YPL226W 1 121767 125357 ON 16 NEW1 (121767..125357) ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction YPL225W 1 126006 126446 ON 16 YPL225W (126006..126446) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YPL224C 0 126634 128088 ON 16 MMT2 complement(126634..128088) Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p YPL223C 0 128632 129138 ON 16 GRE1 complement(128632..129138) Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway YPL222W 1 130162 132228 ON 16 FMP40 (130162..132228) Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YPL222C-A 0 131870 132115 OFF 16 YPL222C-A complement(131870..132115) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL221W 1 133043 135424 ON 16 FLC1 (133043..135424) Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance YPL220W 1 135790 136443 ON 16 RPL1A (135790..136443) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Bp and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal YPL219W 1 136750 138228 ON 16 PCL8 (136750..138228) Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation YPL218W 1 138698 139409 ON 16 SAR1 (138698..138725,138865..139409) GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport YPL217C 0 139620 143171 ON 16 BMS1 complement(139620..143171) GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p YPL216W 1 143821 147129 ON 16 YPL216W (143821..147129) Putative protein of unknown function; YPL216W is not an essential gene YPL215W 1 147416 148423 ON 16 CBP3 (147416..148423) Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex YPL214C 0 148569 150191 ON 16 THI6 complement(148569..150191) Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern YPL213W 1 150614 151330 ON 16 LEA1 (150614..151330) Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein YPL212C 0 151515 153149 ON 16 PUS1 complement(151515..153149) tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA YPL211W 1 153495 154040 ON 16 NIP7 (153495..154040) Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p YPL210C 0 154290 156212 ON 16 SRP72 complement(154290..156212) Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane YPL209C 0 156490 157593 ON 16 IPL1 complement(156490..157593) Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation YPL208W 1 157842 159593 ON 16 RKM1 (157842..159593) SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) YPL207W 1 159909 162341 ON 16 TYW1 (159909..162341) Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron YPL206C 0 162632 163597 ON 16 PGC1 complement(162632..163597) Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs YPL205C 0 163922 164266 OFF 16 YPL205C complement(163922..164266) Hypothetical protein; deletion of locus affects telomere length YPL204W 1 164276 165760 ON 16 HRR25 (164276..165760) Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) YPL203W 1 166256 167398 ON 16 TPK2 (166256..167398) cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase YPL202C 0 168088 169338 ON 16 AFT2 complement(168088..169338) Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; similar to Aft1p YPL201C 0 169770 171155 ON 16 YIG1 complement(169770..171155) Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol YPL200W 1 171484 171954 ON 16 CSM4 (171484..171954) Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements YPL199C 0 172033 172755 ON 16 YPL199C complement(172033..172755) Putative protein of unknown function, predicted to be palmitoylated YPL198W 1 173152 174702 ON 16 RPL7B (173152..173162,173572..173665,174073..174702) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) Scer_snR59 1 173827 173904 OFF 16 SNR59 (173827..173904) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position A807 YPL197C 0 174343 174756 OFF 16 YPL197C complement(174343..174756) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B YPL196W 1 175043 175864 ON 16 OXR1 (175043..175864) Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes YPL195W 1 176223 179021 ON 16 APL5 (176223..179021) Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function YPL194W 1 179277 181115 ON 16 DDC1 (179277..181115) DNA damage checkpoint protein, part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate YPL193W 1 181403 182548 ON 16 RSA1 (181403..182548) Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly YPL192C 0 182655 183056 ON 16 PRM3 complement(182655..183056) Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body YPL191C 0 183597 184679 ON 16 YPL191C complement(183597..184679) Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YPL190C 0 185317 187725 ON 16 NAB3 complement(185317..187725) RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing YPL189C-A 0 188307 188513 ON 16 COA2 complement(188307..188513) Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p YPL189W 1 189154 190983 ON 16 GUP2 (189154..190983) Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog YPL188W 1 191406 192650 ON 16 POS5 (191406..192650) Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress YPL187W 1 193648 194145 ON 16 MF(ALPHA)1 (193648..194145) Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor YPL186C 0 194512 195426 ON 16 UIP4 complement(194512..195426) Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope YPL185W 1 195253 195648 OFF 16 YPL185W (195253..195648) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C YPL184C 0 195950 197788 ON 16 MRN1 complement(195950..197788) RNA-binding protein proposed to be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES) YPL183W-A 1 199095 199376 ON 16 RTC6 (199095..199376) Protein involved translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein encoded in the nucleus YPL183C 0 199495 202536 ON 16 RTT10 complement(199495..202536) WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; has a role in regulation of Ty1 transposition; human ortholog is WDR6 YPL182C 0 203306 203689 OFF 16 YPL182C complement(203306..203689) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W YPL181W 1 203421 204941 ON 16 CTI6 (203421..204941) Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain YPL180W 1 205248 207647 ON 16 TCO89 (205248..207647) Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin YPL179W 1 208157 209806 ON 16 PPQ1 (208157..209806) Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Scer_16.trna2E 0 210192 210263 ON 16 tE-UUC complement(210192..210263) Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin; tRNA Type: Glu (E) | Anti-Codon: UUC | Cove Score: 62.99 YPL178W 1 212158 212784 ON 16 CBC2 (212158..212784) Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif YPL177C 0 213042 213962 ON 16 CUP9 complement(213042..213962) Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription YPL176C 0 216012 218363 ON 16 TRE1 complement(216012..218363) Plasma membrane protein that binds to Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; function is redundant with that of Tre2p; has similarity to transferrin receptors YPL175W 1 218630 220088 ON 16 SPT14 (218630..218646,218747..220088) UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins YPL174C 0 220167 222773 ON 16 NIP100 complement(220167..222773) Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) YPL173W 1 223143 224036 ON 16 MRPL40 (223143..224036) Mitochondrial ribosomal protein of the large subunit YPL172C 0 224353 225741 ON 16 COX10 complement(224353..225741) Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders YPL171C 0 226169 227371 ON 16 OYE3 complement(226169..227371) Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death YPL170W 1 228314 228772 ON 16 DAP1 (228314..228772) Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis YPL169C 0 229039 230838 ON 16 MEX67 complement(229039..230838) Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP YPL168W 1 231217 232509 ON 16 YPL168W (231217..232509) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated YPL167C 0 232593 237107 ON 16 REV3 complement(232593..237107) Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair YPL166W 1 237338 237979 ON 16 ATG29 (237338..237979) Autophagy-specific protein that is required for recruitment of other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved YPL165C 0 237956 239077 ON 16 SET6 complement(237956..239077) SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability YPL164C 0 239350 241497 ON 16 MLH3 complement(239350..241497) Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability YPL163C 0 241918 242700 ON 16 SVS1 complement(241918..242700) Cell wall and vacuolar protein, required for wild-type resistance to vanadate YPL162C 0 243206 244027 ON 16 YPL162C complement(243206..244027) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology YPL161C 0 244319 246220 ON 16 BEM4 complement(244319..246220) Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length YPL160W 1 246990 250262 ON 16 CDC60 (246990..250262) Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA YPL159C 0 250907 251668 ON 16 PET20 complement(250907..251668) Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome YPL158C 0 252034 254310 ON 16 AIM44 complement(252034..254310) Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss YPL157W 1 254814 255761 ON 16 TGS1 (254814..255761) Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs YPL156C 0 255913 256767 ON 16 PRM4 complement(255913..256767) Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift YPL155C 0 257216 259336 ON 16 KIP2 complement(257216..259336) Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle YPL154C 0 259714 260931 ON 16 PEP4 complement(259714..260931) Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates YPL153C 0 261727 264192 ON 16 RAD53 complement(261727..264192) Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication YPL152W-A 1 264602 264700 OFF 16 YPL152W-A (264602..264700) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL152W 1 265028 266104 ON 16 RRD2 (265028..266104) Activator of the phosphotyrosyl phosphatase activity of PP2A,peptidyl-prolyl cis/trans-isomerase; regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex YPL151C 0 266180 267535 ON 16 PRP46 complement(266180..267535) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs YPL150W 1 268188 270893 ON 16 YPL150W (268188..270893) Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study YPL149W 1 271310 272194 ON 16 ATG5 (271310..272194) Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation YPL148C 0 272294 272815 ON 16 PPT2 complement(272294..272815) Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation YPL147W 1 273255 275867 ON 16 PXA1 (273255..275867) Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder YPL146C 0 276162 277529 ON 16 NOP53 complement(276162..277529) Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired YPL145C 0 278395 279699 ON 16 KES1 complement(278395..279699) Member of the oxysterol binding protein family, which includes seven yeast homologs; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex YPL144W 1 280480 280926 ON 16 POC4 (280480..280926) Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Scer_snR17b 0 281056 281517 ON 16 SNR17B complement(281056..281373,281504..281517) small nucleolar RNA (snoRNA) U3; part of small (ribosomal) subunit (SSU) processosome; SNR17A also encodes snoRNA U3 YPL143W 1 282122 282970 ON 16 RPL33A (282122..282140,282666..282970) N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable YPL142C 0 282686 283003 OFF 16 YPL142C complement(282686..283003) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit YPL141C 0 283464 286061 ON 16 FRK1 complement(283464..286061) Protein kinase of unknown cellular role; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3 YPL140C 0 287514 289034 ON 16 MKK2 complement(287514..289034) Mitogen-activated kinase kinase involved in protein kinase C signaling pathway that controls cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p YPL139C 0 289669 291051 ON 16 UME1 complement(289669..291051) Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p YPL138C 0 291366 292427 ON 16 SPP1 complement(291366..292427) Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein YPL137C 0 292817 296647 ON 16 GIP3 complement(292817..296647) Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments YPL136W 1 296286 296654 OFF 16 YPL136W (296286..296654) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C YPL135W 1 297553 298050 ON 16 ISU1 (297553..298050) Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable YPL135C-A 0 297678 297851 OFF 16 YPL135C-A complement(297678..297851) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPL134C 0 298571 299503 ON 16 ODC1 complement(298571..299503) Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation YPL133C 0 299888 301228 ON 16 RDS2 complement(299888..301228) Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole YPL132W 1 301716 302618 ON 16 COX11 (301716..302618) Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p YPL131W 1 303121 304014 ON 16 RPL5 (303121..304014) Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly YPL130W 1 304387 305058 ON 16 SPO19 (304387..305058) Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation YPL129W 1 305298 306137 ON 16 TAF14 (305298..305306,305412..306137) Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes, involved in RNA polymerase II transcription initiation and in chromatin modification; contains a YEATS domain YPL128C 0 306533 308221 ON 16 TBF1 complement(306533..308221) Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation YPL127C 0 308828 309604 ON 16 HHO1 complement(308828..309604) Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation YPL126W 1 310210 312900 ON 16 NAN1 (310210..312900) U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA YPL125W 1 313388 316486 ON 16 KAP120 (313388..316486) Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p YPL124W 1 316755 317516 ON 16 SPC29 (316755..317516) Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication YPL123C 0 317641 318945 ON 16 RNY1 complement(317641..318945) Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity YPL122C 0 319226 320767 ON 16 TFB2 complement(319226..320767) Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH YPL121C 0 320962 321630 ON 16 MEI5 complement(320962..321630) Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p YPL120W 1 322071 323744 ON 16 VPS30 (322071..323744) Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; ortholog of the higher eukaryotic gene Beclin 1 YPL119C-A 0 324024 324287 OFF 16 YPL119C-A complement(324024..324287) Putative protein of unknown function; identified by expression profiling and mass spectrometry YPL119C 0 324411 326264 ON 16 DBP1 complement(324411..326264) Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs YPL118W 1 326628 327662 ON 16 MRP51 (326628..327662) Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences YPL117C 0 327864 328730 ON 16 IDI1 complement(327864..328730) Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability YPL116W 1 329606 331699 ON 16 HOS3 (329606..331699) Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats YPL115C 0 332100 335486 ON 16 BEM3 complement(332100..335486) Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly YPL114W 1 335948 336367 OFF 16 YPL114W (335948..336367) Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index YPL113C 0 335953 337143 ON 16 YPL113C complement(335953..337143) Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene YPL112C 0 337436 338620 ON 16 PEX25 complement(337436..338620) Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Scer_16.trna3M 0 338848 338919 ON 16 tM-CAU complement(338848..338919) Methionine initiator tRNA (tRNA-Met), predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation; tRNA Type: Met (M) | Anti-Codon: CAU | Cove Score: 74.96 YPL111W 1 339944 340945 ON 16 CAR1 (339944..340945) Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance YPL110C 0 341068 344739 ON 16 GDE1 complement(341068..344739) Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes YPL109C 0 345264 347389 ON 16 YPL109C complement(345264..345444,345597..347389) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YPL108W 1 348446 348952 ON 16 YPL108W (348446..348952) Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YPL107W 1 349119 349865 ON 16 YPL107W (349119..349865) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene YPL106C 0 350194 352275 ON 16 SSE1 complement(350194..352275) ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm YPL105C 0 352863 355412 ON 16 SYH1 complement(352863..355412) Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan YPL104W 1 355700 357676 ON 16 MSD1 (355700..357676) Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene YPL103C 0 358000 359406 ON 16 FMP30 complement(358000..359406) Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D YPL102C 0 360003 360305 OFF 16 YPL102C complement(360003..360305) Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 YPL101W 1 360209 361579 ON 16 ELP4 (360209..361579) Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity YPL100W 1 361872 363362 ON 16 ATG21 (361872..363362) Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein YPL099C 0 363520 364068 ON 16 AIM43 complement(363520..364068) Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss YPL098C 0 364386 364727 ON 16 MGR2 complement(364386..364727) Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) YPL097W 1 364952 366430 ON 16 MSY1 (364952..366430) Mitochondrial tyrosyl-tRNA synthetase YPL096C-A 0 366529 366735 ON 16 ERI1 complement(366529..366735) Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein YPL096W 1 366927 368018 ON 16 PNG1 (366927..368018) Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p YPL095C 0 368071 369441 ON 16 EEB1 complement(368071..369441) Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification YPL094C 0 369839 370663 ON 16 SEC62 complement(369839..370663) Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YPL093W 1 370978 372921 ON 16 NOG1 (370978..372921) Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins YPL092W 1 373793 375169 ON 16 SSU1 (373793..375169) Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein YPL091W 1 375502 376953 ON 16 GLR1 (375502..376953) Cytosolic and mitochondrial glutathione oxidoreductase, converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia YPL090C 0 377291 378395 ON 16 RPS6A complement(377291..377995,378390..378395) Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein YPL089C 0 379120 381150 ON 16 RLM1 complement(379120..381150) MADS-box transcription factor, component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p YPL088W 1 381965 382993 ON 16 YPL088W (381965..382993) Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance YPL087W 1 383455 384408 ON 16 YDC1 (383455..384408) Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity YPL086C 0 384773 386446 ON 16 ELP3 complement(384773..386446) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin YPL085W 1 387067 393654 ON 16 SEC16 (387067..393654) COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p YPL084W 1 394038 396572 ON 16 BRO1 (394038..396572) Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes YPL083C 0 396702 398105 ON 16 SEN54 complement(396702..398105) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p YPL082C 0 398480 404083 ON 16 MOT1 complement(398480..404083) Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity YPL081W 1 404950 406044 ON 16 RPS9A (404950..404956,405458..406044) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Bp and has similarity to E. coli S4 and rat S9 ribosomal proteins YPL080C 0 406172 406498 OFF 16 YPL080C complement(406172..406498) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YPL079W 1 406636 407539 ON 16 RPL21B (406636..406646,407068..407539) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat L21 ribosomal protein YPL078C 0 408010 408744 ON 16 ATP4 complement(408010..408744) Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated YPL077C 0 409368 410090 ON 16 YPL077C complement(409368..410090) Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene YPL076W 1 410443 411285 ON 16 GPI2 (410443..411285) Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein YPL075W 1 412254 415362 ON 16 GCR1 (412254..412261,413013..415362) Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p YPL074W 1 415763 418027 ON 16 YTA6 (415763..418027) Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localized to the cortex of mother cells but not to daughter cells YPL073C 0 418499 418984 OFF 16 YPL073C complement(418499..418984) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments YPL072W 1 418512 420011 ON 16 UBP16 (418512..420011) Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria YPL071C 0 420048 420518 ON 16 YPL071C complement(420048..420518) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YPL070W 1 420948 422786 ON 16 MUK1 (420948..422786) Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation YPL069C 0 422885 423892 ON 16 BTS1 complement(422885..423892) Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic YPL068C 0 424215 425096 ON 16 YPL068C complement(424215..425096) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS YPL067C 0 425251 425847 ON 16 YPL067C complement(425251..425847) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene YPL066W 1 426233 427672 ON 16 RGL1 (426233..427672) Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source YPL065W 1 427927 428655 ON 16 VPS28 (427927..428655) Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p YPL064C 0 428711 429616 ON 16 CWC27 complement(428711..429616) Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p YPL063W 1 429939 431369 ON 16 TIM50 (429939..431369) Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel YPL062W 1 431895 432299 OFF 16 YPL062W (431895..432299) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation YPL061W 1 432588 434090 ON 16 ALD6 (432588..434090) Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress YPL060W 1 434523 435764 ON 16 MFM1 (434523..435764) Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Scer_16.trna4C 0 435893 435964 ON 16 tC-GCA complement(435893..435964) Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis; tRNA Type: Cys (C) | Anti-Codon: GCA | Cove Score: 75.04 YPL060C-A 0 439428 442742 OFF 16 YPL060C-A complement(439428..442742) TyA Gag protein; the main structural constituent of virus-like particles (VLPs) YPL059W 1 444579 445031 ON 16 GRX5 (444579..445031) Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p YPL058C 0 445842 450377 ON 16 PDR12 complement(445842..450377) Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity YPL057C 0 451909 453057 ON 16 SUR1 complement(451909..453057) Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p YPL056C 0 453430 453735 OFF 16 LCL1 complement(453430..453735) Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan YPL055C 0 453992 454990 ON 16 LGE1 complement(453992..454990) Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division YPL054W 1 455759 456664 ON 16 LEE1 (455759..456664) Zinc-finger protein of unknown function YPL053C 0 457118 458458 ON 16 KTR6 complement(457118..458458) Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family YPL052W 1 458799 459678 ON 16 OAZ1 (458799..459005,459007..459678) Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p YPL051W 1 459963 460559 ON 16 ARL3 (459963..460559) GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor YPL050C 0 460779 461966 ON 16 MNN9 complement(460779..461966) Subunit of Golgi mannosyltransferase complex also containing Anp1p, Mnn10p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation YPL049C 0 462481 463839 ON 16 DIG1 complement(462481..463839) MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p YPL048W 1 464401 465648 ON 16 CAM1 (464401..465648) Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma YPL047W 1 465962 466261 ON 16 SGF11 (465962..466261) Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation YPL046C 0 466644 466943 ON 16 ELC1 complement(466644..466943) Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair YPL045W 1 467260 469656 ON 16 VPS16 (467260..469656) Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages YPL044C 0 469926 470474 OFF 16 YPL044C complement(469926..470474) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W YPL043W 1 469939 471996 ON 16 NOP4 (469939..471996) Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) YPL042C 0 473040 474707 ON 16 SSN3 complement(473040..474707) Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression YPL041C 0 475118 475741 ON 16 YPL041C complement(475118..475741) Protein of unknown function involved in maintenance of proper telomere length YPL040C 0 475993 479001 ON 16 ISM1 complement(475993..479001) Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth YPL039W 1 479225 480175 ON 16 YPL039W (479225..480175) Putative protein of unknown function; YPL039W is not an essential gene YPL038W-A 1 480182 480373 OFF 16 YPL038W-A (480182..480373) Putative protein of unknown function; identified by fungal homology and RT-PCR YPL038W 1 480535 481068 ON 16 MET31 (480535..481068) Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met32p YPL037C 0 481428 481901 ON 16 EGD1 complement(481428..481901) Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b YPL036W 1 482843 485686 ON 16 PMA2 (482843..485686) Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential YPL035C 0 486564 486911 OFF 16 YPL035C complement(486564..486911) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene YPL034W 1 486712 487209 ON 16 YPL034W (486712..487209) Putative protein of unknown function; YPL034W is not essential gene YPL033C 0 487362 488207 ON 16 SRL4 complement(487362..488207) Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p YPL032C 0 488887 491364 ON 16 SVL3 complement(488887..491364) Protein of unknown function, mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck YPL031C 0 492018 493037 ON 16 PHO85 complement(492018..492918,493021..493037) Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle YPL030W 1 493543 495246 ON 16 TRM44 (493543..495246) tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene YPL029W 1 495506 497719 ON 16 SUV3 (495506..497719) ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron YPL028W 1 498096 499292 ON 16 ERG10 (498096..499292) Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis YPL027W 1 499665 500402 ON 16 SMA1 (499665..500402) Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p YPL026C 0 500675 502183 ON 16 SKS1 complement(500675..502183) Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway YPL025C 0 502473 503030 OFF 16 YPL025C complement(502473..503030) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YPL024W 1 503517 504242 ON 16 RMI1 (503517..504242) Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing and ssDNA binding activities of Top3p; involved in response to DNA damage; null mutants display increased rates of recombination and delayed S phase YPL023C 0 504339 506312 ON 16 MET12 complement(504339..506312) Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR YPL022W 1 506697 509999 ON 16 RAD1 (506697..509999) Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein YPL021W 1 511101 511664 ON 16 ECM23 (511101..511664) Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p YPL020C 0 512313 514178 ON 16 ULP1 complement(512313..514178) Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions YPL019C 0 514511 517018 ON 16 VTC3 complement(514511..517018) Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion YPL018W 1 517651 518760 ON 16 CTF19 (517651..518760) Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly YPL017C 0 518734 520233 ON 16 IRC15 complement(518734..520233) Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci YPL016W 1 521014 524958 ON 16 SWI1 (521014..524958) Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer YPL015C 0 525810 526883 ON 16 HST2 complement(525810..526883) Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro YPL014W 1 527547 528692 ON 16 YPL014W (527547..528692) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus YPL013C 0 528985 529350 ON 16 MRPS16 complement(528985..529350) Mitochondrial ribosomal protein of the small subunit YPL012W 1 529723 533409 ON 16 RRP12 (529723..533409) Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats YPL011C 0 533642 534703 ON 16 TAF3 complement(533642..534703) TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation YPL010W 1 535018 535587 ON 16 RET3 (535018..535587) Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER YPL009C 0 535820 538936 ON 16 TAE2 complement(535820..538936) Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YPL008W 1 539385 541970 ON 16 CHL1 (539385..541970) Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome YPL007C 0 542082 543848 ON 16 TFC8 complement(542082..543848) One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 YPL006W 1 544631 548143 ON 16 NCR1 (544631..548143) Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein YPL005W 1 548486 550306 ON 16 AEP3 (548486..550306) Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA YPL004C 0 550632 551657 ON 16 LSP1 complement(550632..551657) Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family YPL003W 1 552020 553408 ON 16 ULA1 (552020..553408) Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation YPL002C 0 553627 554328 ON 16 SNF8 complement(553627..554328) Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression YPL001W 1 554605 555729 ON 16 HAT1 (554605..555729) Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Scer_CEN16 1 555957 556073 ON 16 CEN16 (555957..556073) Chromosome XVI centromere YPR001W 1 556377 557837 ON 16 CIT3 (556377..557837) Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate YPR002W 1 558385 559935 ON 16 PDH1 (558385..559935) Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Scer_16.trna5F 0 560198 560289 ON 16 tF-GAA complement(560198..560233,560253..560289) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YPR002C-A 0 560569 560766 OFF 16 YPR002C-A complement(560569..560766) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the Ty1 long terminal repeat, YPRWdelta12 YPR003C 0 561504 563768 ON 16 YPR003C complement(561504..563768) Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene YPR004C 0 564007 565041 ON 16 AIM45 complement(564007..565041) Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response YPR005C 0 565787 566671 ON 16 HAL1 complement(565787..566671) Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p YPR006C 0 567269 568996 ON 16 ICL2 complement(567269..568996) 2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol YPR007C 0 569336 571378 ON 16 REC8 complement(569336..571378) Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Scer_16.trna6G 1 572269 572339 ON 16 tG-GCC (572269..572339) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YPR008W 1 573018 575102 ON 16 HAA1 (573018..575102) Transcriptional activator involved in the transcription of TPO2, YRO2, and other genes putatively encoding membrane stress proteins; involved in adaptation to weak acid stress YPR009W 1 576552 577358 ON 16 SUT2 (576552..577358) Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p YPR010C 0 577585 581196 ON 16 RPA135 complement(577585..581196) RNA polymerase I second largest subunit A135 Scer_16.trna7K 1 582062 582134 ON 16 tK-CUU (582062..582134) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced; tRNA Type: Lys (K) | Anti-Codon: CUU | Cove Score: 77.79 YPR010C-A 0 582373 582734 ON 16 YPR010C-A complement(582373..582558,582702..582734) Putative protein of unknown function; conserved among Saccharomyces sensu stricto species YPR011C 0 583062 584042 ON 16 YPR011C complement(583062..584042) Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YPR012W 1 584309 584563 OFF 16 YPR012W (584309..584563) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene YPR013C 0 584632 585585 ON 16 YPR013C complement(584632..585585) Putative zinc finger protein; YPR013C is not an essential gene YPR014C 0 587189 587518 OFF 16 YPR014C complement(587189..587518) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene YPR015C 0 590283 591026 ON 16 YPR015C complement(590283..591026) Putative protein of unknown function; overexpression causes a cell cycle delay or arrest YPR016C 0 592332 593069 ON 16 TIF6 complement(592332..593069) Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits YPR016W-A 1 593096 593356 OFF 16 YPR016W-A (593096..593356) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YPR017C 0 593486 593917 ON 16 DSS4 complement(593486..593917) Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol YPR018W 1 594476 596296 ON 16 RLF2 (594476..596296) Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription YPR019W 1 596750 599551 ON 16 MCM4 (596750..599551) Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p YPR020W 1 599870 600217 ON 16 ATP20 (599870..600217) Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex YPR021C 0 600649 603357 ON 16 AGC1 complement(600649..603357) Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis YPR022C 0 603911 607312 ON 16 YPR022C complement(603911..607312) Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS YPR023C 0 608826 610031 ON 16 EAF3 complement(608826..610031) Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition YPR024W 1 610481 612724 ON 16 YME1 (610481..612724) Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; mutation causes an elevated rate of mitochondrial turnover YPR025C 0 613377 614558 ON 16 CCL1 complement(613377..614558) Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters YPR026W 1 615379 619014 ON 16 ATH1 (615379..619014) Acid trehalase required for utilization of extracellular trehalose YPR027C 0 620425 621258 ON 16 YPR027C complement(620425..621258) Putative protein of unknown function Scer_16.trna8F 0 622540 622631 ON 16 tF-GAA complement(622540..622575,622595..622631) Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis; tRNA Type: Phe (F) | Anti-Codon: GAA | Cove Score: 69.79 YPR028W 1 623527 624202 ON 16 YOP1 (623527..623577,623711..624202) Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes YPR029C 0 624469 626967 ON 16 APL4 complement(624469..626967) Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport YPR030W 1 627880 631245 ON 16 CSR2 (627880..631245) Nuclear protein proposed to regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p YPR031W 1 631515 633761 ON 16 NTO1 (631515..633761) Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 YPR032W 1 634123 637224 ON 16 SRO7 (634123..637224) Effector of Rab GTPase Sec4p, forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro77p and Drosophila lgl tumor suppressor YPR033C 0 637379 639019 ON 16 HTS1 complement(637379..639019) Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome YPR034W 1 639525 640958 ON 16 ARP7 (639525..640958) Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation YPR035W 1 642208 643320 ON 16 GLN1 (642208..643320) Glutamine synthetase (GS), synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation YPR036W 1 643836 645272 ON 16 VMA13 (643836..645272) Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase YPR036W-A 1 645950 646153 ON 16 YPR036W-A (645950..646153) Protein of unknown function; transcription is regulated by Pdr1p YPR037C 0 646448 647038 ON 16 ERV2 complement(646448..647038) Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER YPR038W 1 646836 647195 OFF 16 IRC16 (646836..647195) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci YPR039W 1 647012 647347 OFF 16 YPR039W (647012..647347) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W YPR040W 1 647305 648375 ON 16 TIP41 (647305..648375) Protein that interacts physically and genetically with Tap42p, which regulates protein phosphatase 2A; component of the TOR (target of rapamycin) signaling pathway YPR041W 1 648704 649921 ON 16 TIF5 (648704..649921) Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 YPR042C 0 650435 653662 ON 16 PUF2 complement(650435..653662) Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins YPR043W 1 654166 654847 ON 16 RPL43A (654166..654167,654571..654847) Protein component of the large (60S) ribosomal subunit, identical to Rpl43Bp and has similarity to rat L37a ribosomal protein; null mutation confers a dominant lethal phenotype YPR044C 0 654524 654877 OFF 16 OPI11 complement(654524..654877) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO YPR045C 0 655140 656552 ON 16 THP3 complement(655140..656552) Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes YPR046W 1 656799 657344 ON 16 MCM16 (656799..657344) Protein involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA YPR047W 1 657529 658938 ON 16 MSF1 (657529..658938) Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase YPR048W 1 659182 661053 ON 16 TAH18 (659182..661053) Conserved NAPDH-dependent diflavin reductase, component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress YPR049C 0 661137 664673 ON 16 ATG11 complement(661137..664673) Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) YPR050C 0 664953 665366 OFF 16 YPR050C complement(664953..665366) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W YPR051W 1 664960 665490 ON 16 MAK3 (664960..665490) Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus YPR052C 0 665693 665974 ON 16 NHP6A complement(665693..665974) High-mobility group (HMG) protein that binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2 YPR053C 0 665788 666243 OFF 16 YPR053C complement(665788..666243) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C YPR054W 1 666280 667446 ON 16 SMK1 (666280..667446) Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p YPR055W 1 667676 670873 ON 16 SEC8 (667676..670873) Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YPR056W 1 671126 672142 ON 16 TFB4 (671126..672142) Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p YPR057W 1 672471 673496 ON 16 BRR1 (672471..673496) snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed YPR058W 1 673751 674674 ON 16 YMC1 (673751..674674) Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc2p YPR059C 0 674314 674700 OFF 16 YPR059C complement(674314..674700) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W YPR060C 0 674861 675631 ON 16 ARO7 complement(674861..675631) Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis YPR061C 0 675977 676882 ON 16 JID1 complement(675977..676882) Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae YPR062W 1 677165 677641 ON 16 FCY1 (677165..677641) Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) YPR063C 0 677812 678320 ON 16 YPR063C complement(677812..678193,678280..678320) ER-localized protein of unknown function YPR064W 1 678951 679370 OFF 16 YPR064W (678951..679370) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR065W 1 679693 680799 ON 16 ROX1 (679693..680799) Heme-dependent repressor of hypoxic genes; contains an HMG domain that is responsible for DNA bending activity YPR066W 1 681213 682112 ON 16 UBA3 (681213..682112) Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern YPR067W 1 682220 682777 ON 16 ISA2 (682220..682777) Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations YPR068C 0 682941 684353 ON 16 HOS1 complement(682941..684353) Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex YPR069C 0 684556 685437 ON 16 SPE3 complement(684556..685437) Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells YPR070W 1 685898 687598 ON 16 MED1 (685898..687598) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YPR071W 1 688172 688807 ON 16 YPR071W (688172..688807) Putative membrane protein; YPR071W is not an essential gene Scer_16.trna9S 1 689565 689646 ON 16 tS-UGA (689565..689646) Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations; tRNA Type: Ser (S) | Anti-Codon: UGA | Cove Score: 84.16 YPR072W 1 690107 691789 ON 16 NOT5 (690107..691789) Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation YPR073C 0 691933 692418 ON 16 LTP1 complement(691933..692418) Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine YPR074C 0 692796 694838 ON 16 TKL1 complement(692796..694838) Transketolase, similar to Tkl2p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids YPR074W-A 1 695018 695188 OFF 16 YPR074W-A (695018..695188) Hypothetical protein identified by homology YPR075C 0 695737 696819 ON 16 OPY2 complement(695737..696819) Integral membrane protein that functions in the signaling branch of the high-osmolarity glycerol (HOG) pathway; interacts with Ste50p; overproduction blocks cell cycle arrest in the presence of mating pheromone YPR076W 1 696476 696850 OFF 16 YPR076W (696476..696850) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR077C 0 697131 697502 OFF 16 YPR077C complement(697131..697502) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 YPR078C 0 697147 698265 ON 16 YPR078C complement(697147..698265) Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible YPR079W 1 698869 700014 ON 16 MRL1 (698869..700014) Membrane protein with similarity to mammalian mannose-6-phosphate receptors, possibly functions as a sorting receptor in the delivery of vacuolar hydrolases YPR080W 1 700594 701970 ON 16 TEF1 (700594..701970) Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus YPR081C 0 702114 703970 ON 16 GRS2 complement(702114..703970) Glycine-tRNA synthetase, not expressed under normal growth conditions; expression is induced under heat, oxidative, pH, or ethanol stress conditions; more stable than the major glycine-tRNA synthetase Grs1p at 37 deg C YPR082C 0 704225 704656 ON 16 DIB1 complement(704225..704656) 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein YPR083W 1 704854 706593 ON 16 MDM36 (704854..706593) Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission YPR084W 1 706972 708342 ON 16 YPR084W (706972..708342) Putative protein of unknown function YPR085C 0 708497 709828 ON 16 ASA1 complement(708497..709828) Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 YPR086W 1 710101 711138 ON 16 SUA7 (710101..711138) Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II YPR087W 1 711354 711674 OFF 16 VPS69 (711354..711674) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect YPR088C 0 711403 713028 ON 16 SRP54 complement(711403..713028) Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain YPR089W 1 713275 715941 ON 16 YPR089W (713275..715941) Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p YPR091C 0 716156 718468 ON 16 NVJ2 complement(716156..718468) Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments YPR092W 1 718379 718684 OFF 16 YPR092W (718379..718684) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Scer_snR51 0 718700 718806 ON 16 SNR51 complement(718700..718806) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of large subunit (LSU) rRNA at position U2729 and small subunit (SSU) rRNA at position A100 Scer_snR70 0 718887 719050 ON 16 SNR70 complement(718887..719050) C/D box small nucleolar RNA (snoRNA), predicted to guide 2'-O-methylation of small subunit (SSU) rRNA at position C1639 Scer_snR41 0 719148 719242 ON 16 SNR41 complement(719148..719242) C/D box small nucleolar RNA (snoRNA), guides 2'-O-methylation of small subunit (SSU) rRNA at positions A541 and G1126 YPR093C 0 719558 720424 ON 16 ASR1 complement(719558..720424) Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein YPR094W 1 720637 720960 ON 16 RDS3 (720637..720960) Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance YPR095C 0 721037 724717 ON 16 SYT1 complement(721037..724717) Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain YPR096C 0 724842 725144 OFF 16 YPR096C complement(724842..725144) Protein of unknown function that may interact with ribosomes, based on co-purification experiments YPR097W 1 725394 728615 ON 16 YPR097W (725394..728615) Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YPR098C 0 728947 729528 ON 16 YPR098C complement(728947..729385,729482..729528) Protein of unknown function, localized to the mitochondrial outer membrane YPR099C 0 729757 730113 OFF 16 YPR099C complement(729757..730113) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W YPR100W 1 729791 730213 ON 16 MRPL51 (729791..730213) Mitochondrial ribosomal protein of the large subunit YPR101W 1 730492 731019 ON 16 SNT309 (730492..731019) Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p YPR102C 0 731224 731748 ON 16 RPL11A complement(731224..731748) Protein of the large 60S ribosomal subunit, nearly identical to Rpl11Bp but expressed at twice the level; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; similar to E. coli L5 and rat L11 YPR103W 1 732349 733212 ON 16 PRE2 (732349..733212) Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome YPR104C 0 733623 736433 ON 16 FHL1 complement(733623..736433) Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants YPR105C 0 736984 739569 ON 16 COG4 complement(736984..739569) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YPR106W 1 740061 741392 ON 16 ISR1 (740061..741392) Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C YPR107C 0 741438 742064 ON 16 YTH1 complement(741438..742064) Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation YPR108W 1 742454 743743 ON 16 RPN7 (742454..743743) Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits YPR108W-A 1 744175 744387 OFF 16 YPR108W-A (744175..744387) Putative protein of unknown function; identified by fungal homology and RT-PCR Scer_16.trna10T 1 744284 744355 ON 16 tT-UGU (744284..744355) Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; tRNA Type: Thr (T) | Anti-Codon: UGU | Cove Score: 77.84 YPR109W 1 744689 745573 ON 16 YPR109W (744689..745573) Predicted membrane protein; dipoid deletion strain has high budding index YPR110C 0 745828 746835 ON 16 RPC40 complement(745828..746835) RNA polymerase subunit AC40, common to RNA polymerase I and III YPR111W 1 747306 749000 ON 16 DBF20 (747306..749000) Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs YPR112C 0 749256 751919 ON 16 MRD1 complement(749256..751919) Essential conserved protein that is part of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains YPR113W 1 752257 752919 ON 16 PIS1 (752257..752919) Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins YPR114W 1 753301 754248 ON 16 YPR114W (753301..754248) Putative protein of unknown function YPR115W 1 754877 758128 ON 16 RGC1 (754877..758128) Pleckstrin homology domain containing protein proposed to function as a glycerol channel activator; paralogous to Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YPR116W 1 758648 759481 ON 16 RRG8 (758648..759481) Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport YPR117W 1 760025 767494 ON 16 YPR117W (760025..767494) Putative protein of unknown function YPR118W 1 767752 768987 ON 16 MRI1 (767752..768987) 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Scer_16.trna11K 0 769207 769302 ON 16 tK-UUU complement(769207..769242,769266..769302) Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; tRNA Type: Lys (K) | Anti-Codon: UUU | Cove Score: 69.00 YPR119W 1 771653 773128 ON 16 CLB2 (771653..773128) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome YPR120C 0 773875 775182 ON 16 CLB5 complement(773875..775182) B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Scer_16.trna12C 0 775765 775836 ON 16 tC-GCA complement(775765..775836) Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis; tRNA Type: Cys (C) | Anti-Codon: GCA | Cove Score: 75.04 YPR121W 1 778583 780301 ON 16 THI22 (778583..780301) Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis YPR122W 1 782045 785671 ON 16 AXL1 (782045..785671) Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells YPR123C 0 786141 786575 OFF 16 YPR123C complement(786141..786575) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR YPR124W 1 786208 787428 ON 16 CTR1 (786208..787428) High-affinity copper transporter of the plasma membrane, mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels YPR125W 1 787961 789325 ON 16 YLH47 (787961..789325) Mitochondrial inner membrane protein exposed to the mitochondrial matrix, associates with mitochondrial ribosomes, NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome YPR126C 0 789077 789385 OFF 16 YPR126C complement(789077..789385) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR127W 1 790083 791120 ON 16 YPR127W (790083..791120) Protein of unknown function, differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YPR128C 0 791218 792204 ON 16 ANT1 complement(791218..792204) Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation YPR129W 1 792687 793736 ON 16 SCD6 (792687..793736) Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1 YPR130C 0 793374 793781 OFF 16 YPR130C complement(793374..793781) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR131C 0 793911 794498 ON 16 NAT3 complement(793911..794498) Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met YPR132W 1 794965 795767 ON 16 RPS23B (794965..795029,795395..795767) Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Ap and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal YPR133C 0 795978 797210 ON 16 SPN1 complement(795978..797210) Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype YPR133W-A 1 797557 797709 ON 16 TOM5 (797557..797709) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore YPR134W 1 798051 798857 ON 16 MSS18 (798051..798857) Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions YPR135W 1 799234 802017 ON 16 CTF4 (799234..802017) Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion YPR136C 0 802325 802837 OFF 16 YPR136C complement(802325..802837) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 YPR137W 1 802359 804080 ON 16 RRP9 (802359..804080) Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Scer_16.trna13N 1 810676 810749 ON 16 tN-GUU (810676..810749) Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis; tRNA Type: Asn (N) | Anti-Codon: GUU | Cove Score: 77.73 YPR138C 0 810984 812453 ON 16 MEP3 complement(810984..812453) Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease YPR139C 0 813156 814058 ON 16 LOA1 complement(813156..814058) lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA YPR140W 1 814391 815536 ON 16 TAZ1 (814391..815536) Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome YPR141C 0 815734 817923 ON 16 KAR3 complement(815734..817923) Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate YPR142C 0 818130 818693 OFF 16 YPR142C complement(818130..818693) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing YPR143W 1 818323 819075 ON 16 RRP15 (818323..819075) Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Scer_16.trna14I 1 819529 819602 ON 16 tI-AAU (819529..819602) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YPR144C 0 819765 821423 ON 16 NOC4 complement(819765..821423) Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Scer_snR45 1 821732 821903 ON 16 SNR45 (821732..821903) C/D box small nucleolar RNA (snoRNA) but not shown to be active and no target site is predicted within the ribosomal RNAs YPR145W 1 822620 824338 ON 16 ASN1 (822620..824338) Asparagine synthetase, isozyme of Asn2p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway YPR145C-A 0 824690 824926 ON 16 YPR145C-A complement(824690..824926) Putative protein of unknown function YPR146C 0 825347 825676 OFF 16 YPR146C complement(825347..825676) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR147C 0 825645 826559 ON 16 YPR147C complement(825645..826559) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS YPR148C 0 826833 828140 ON 16 YPR148C complement(826833..828140) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YPR149W 1 829918 830439 ON 16 NCE102 (829918..830439) Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) YPR150W 1 830999 831520 OFF 16 YPR150W (830999..831520) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C YPR151C 0 831055 831675 ON 16 SUE1 complement(831055..831675) Mitochondrial protein required for degradation of unstable forms of cytochrome c YPR152C 0 832061 833458 ON 16 URN1 complement(832061..833458) Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase YPR153W 1 833689 834245 ON 16 YPR153W (833689..833693,833828..834245) Putative protein of unknown function YPR154W 1 834565 835212 ON 16 PIN3 (834565..835212) Protein that induces appearance of [PIN+] prion when overproduced YPR155C 0 835563 837413 ON 16 NCA2 complement(835563..837413) Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p YPR156C 0 837909 839777 ON 16 TPO3 complement(837909..839777) Polyamine transport protein specific for spermine; localizes to the plasma membrane; member of the major facilitator superfamily YPR157W 1 841266 842669 ON 16 TDA6 (841266..842669) Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele YPR158W 1 843262 844020 ON 16 CUR1 (843262..844020) Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; similar in sequence to Btn2p Scer_16.trna15A 1 856902 856974 ON 16 tA-AGC (856902..856974) Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine; tRNA Type: Ala (A) | Anti-Codon: AGC | Cove Score: 74.48 YPR159W 1 857583 859745 ON 16 KRE6 (857583..859745) Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p YPR159C-A 0 860314 860415 OFF 16 YPR159C-A complement(860314..860415) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Scer_16.trna16G 1 860379 860449 ON 16 tG-GCC (860379..860449) Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; tRNA Type: Gly (G) | Anti-Codon: GCC | Cove Score: 62.73 YPR160W 1 861306 864014 ON 16 GPH1 (861306..864014) Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway YPR160W-A 1 861934 862014 OFF 16 YPR160W-A (861934..862014) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry YPR160C-A 0 862577 862861 OFF 16 YPR160C-A complement(862577..862861) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YPR161C 0 864449 866422 ON 16 SGV1 complement(864449..866422) Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins YPR162C 0 866715 868304 ON 16 ORC4 complement(866715..868304) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing YPR163C 0 868645 869955 ON 16 TIF3 complement(868645..869955) Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA YPR164W 1 870703 874926 ON 16 MMS1 (870703..874926) Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay YPR165W 1 875368 875997 ON 16 RHO1 (875368..875997) GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) YPR166C 0 876282 876629 ON 16 MRP2 complement(876282..876629) Mitochondrial ribosomal protein of the small subunit YPR167C 0 876847 877632 ON 16 MET16 complement(876847..877632) 3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism YPR168W 1 878076 878549 ON 16 NUT2 (878076..878549) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription YPR169W 1 878690 880168 ON 16 JIP5 (878690..880168) Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Scer_16.trna17I 0 880296 880369 ON 16 tI-AAU complement(880296..880369) Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis; tRNA Type: Ile (I) | Anti-Codon: AAU | Cove Score: 71.40 YPR170C 0 882983 883318 OFF 16 YPR170C complement(882983..883318) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B YPR169W-A 1 883239 883457 OFF 16 YPR169W-A (883239..883457) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps two other dubious ORFs: YPR170C and YPR170W-B YPR170W-B 1 883239 883595 ON 16 YPR170W-B (883239..883387,883487..883595) Putative protein of unknown function, conserved in fungi; partially overlaps the dubious genes YPR169W-A, YPR170W-A and YRP170C YPR170W-A 1 883378 883563 OFF 16 YPR170W-A (883378..883563) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by expression profiling and mass spectrometry YPR171W 1 883828 885558 ON 16 BSP1 (883828..885558) Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton YPR172W 1 885796 886398 ON 16 YPR172W (885796..886398) Protein of unknown function, transcriptionally activated by Yrm1p along with genes involved in multidrug resistance YPR173C 0 886524 887837 ON 16 VPS4 complement(886524..887837) AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism YPR174C 0 888043 888708 ON 16 YPR174C complement(888043..888708) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study YPR175W 1 888974 891043 ON 16 DPB2 (888974..891043) Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate YPR176C 0 891101 892078 ON 16 BET2 complement(891101..892078) Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p YPR177C 0 892313 892684 OFF 16 YPR177C complement(892313..892684) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex YPR178W 1 892332 893729 ON 16 PRP4 (892332..893729) Splicing factor, component of the U4/U6-U5 snRNP complex YPR179C 0 893797 895764 ON 16 HDA3 complement(893797..895764) Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p YPR180W 1 895961 897004 ON 16 AOS1 (895961..897004) Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability YPR181C 0 897361 899667 ON 16 SEC23 complement(897361..899667) GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p YPR182W 1 900194 900454 ON 16 SMX3 (900194..900454) Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F YPR183W 1 900755 901558 ON 16 DPM1 (900755..901558) Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation YPR184W 1 902044 906654 ON 16 GDB1 (902044..906654) Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p YPR185W 1 907218 909434 ON 16 ATG13 (907218..909434) Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling YPR186C 0 909733 911022 ON 16 PZF1 complement(909733..911022) Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA YPR187W 1 911257 911800 ON 16 RPO26 (911257..911276,911353..911800) RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit YPR188C 0 911988 912479 ON 16 MLC2 complement(911988..912479) Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring YPR189W 1 912664 916962 ON 16 SKI3 (912664..916962) Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay YPR190C 0 917077 919041 ON 16 RPC82 complement(917077..919041) RNA polymerase III subunit C82 YPR191W 1 919381 920487 ON 16 QCR2 (919381..920487) Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme YPR192W 1 921860 922777 ON 16 AQY1 (921860..922777) Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance YPR193C 0 922909 923379 ON 16 HPA2 complement(922909..923379) Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity YPR194C 0 924304 926937 ON 16 OPT2 complement(924304..926937) Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles YPR195C 0 927965 928294 OFF 16 YPR195C complement(927965..928294) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YPR196W 1 931376 932788 ON 16 YPR196W (931376..932788) Putative maltose-responsive transcription factor YPR197C 0 933898 934461 OFF 16 YPR197C complement(933898..934461) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YPR198W 1 934034 935665 ON 16 SGE1 (934034..935665) Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations YPR199C 0 938148 939032 ON 16 ARR1 complement(938148..939032) Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds YPR200C 0 939279 939671 ON 16 ARR2 complement(939279..939671) Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p YPR201W 1 939922 941136 ON 16 ARR3 (939922..941136) Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite YPR202W 1 943032 943896 ON 16 YPR202W (943032..943050,943199..943896) Putative protein of unknown function with similarity to telomere-encoded helicases; YPR202W is not an essential gene; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo YPR203W 1 943880 944188 ON 16 YPR203W (943880..944188) Putative protein of unknown function YPR204W 1 944603 947701 ON 16 YPR204W (944603..947701) Helicase-like protein encoded within the telomeric Y' element YPR204C-A 0 946856 947338 OFF 16 YPR204C-A complement(946856..947338) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching