YAL069W 1 335 649 OFF 1 YAL069W (335..649) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL068W-A 1 538 792 OFF 1 YAL068W-A (538..792) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL068C 0 1807 2169 ON 1 YAL068C complement(1807..2169) Hypothetical protein YAL067W-A 1 2480 2707 OFF 1 YAL067W-A (2480..2707) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL067C 0 7236 9017 ON 1 SEO1 complement(7236..9017) Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide YAL066W 1 10092 10400 OFF 1 YAL066W (10092..10400) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL065C 0 11566 11952 ON 1 YAL065C complement(11566..11952) Putative protein of unknown function; has homology to FLO1; possible pseudogene YAL064W-B 1 12047 12427 ON 1 YAL064W-B (12047..12427) Fungal-specific protein of unknown function YAL064C-A 0 13364 13744 ON 1 YAL064C-A complement(13364..13744) Putative protein of unknown function; YAL064C-A is not an essential gene YAL064W 1 21526 21852 ON 1 YAL064W (21526..21852) Protein of unknown function; may interact with ribosomes, based on co-purification experiments YAL063C-A 0 22397 22687 OFF 1 YAL063C-A complement(22397..22687) Putative protein of unknown function; identified by expression profiling and mass spectrometry YAL063C 0 24001 27969 ON 1 FLO9 complement(24001..27969) Lectin-like protein with similarity to Flo1p, thought to be expressed and involved in flocculation YAL062W 1 31568 32941 ON 1 GDH3 (31568..32941) NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources YAL061W 1 33449 34702 ON 1 BDH2 (33449..34702) Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3; BDH2 is an essential gene YAL060W 1 35156 36304 ON 1 BDH1 (35156..36304) NAD-dependent (2R,3R)-2,3-butanediol dehydrogenase, a zinc-containing medium-chain alcohol dehydrogenase, produces 2,3-butanediol from acetoin during fermentation and allows using 2,3-butanediol as a carbon source during aerobic growth YAL059C-A 0 36497 36919 OFF 1 YAL059C-A complement(36497..36919) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene ECM1/YAL059W YAL059W 1 36510 37148 ON 1 ECM1 (36510..37148) Protein of unknown function, localized in the nucleoplasm and the nucleolus, genetically interacts with MTR2 in 60S ribosomal protein subunit export YAL058W 1 37465 38973 ON 1 CNE1 (37465..38973) Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast YAL056C-A 0 38697 39047 OFF 1 YAL056C-A complement(38697..39047) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL056W 1 39260 41902 ON 1 GPB2 (39260..41902) Proposed beta subunit of the heterotrimeric G protein; interacts with the receptor Grp1p, affects signaling by altering the level of phosphorylation of PKS substrates; regulates pseudohyphal growth through cAMP levels; homolog of Gpb1p YAL055W 1 42178 42720 ON 1 PEX22 (42178..42720) Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation YAL054C 0 42882 45023 ON 1 ACS1 complement(42882..45023) Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetlyation; expressed during growth on nonfermentable carbon sources and under aerobic conditions YAL053W 1 45900 48251 ON 1 FLC2 (45900..48251) Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance YAL051W 1 48565 51708 ON 1 OAF1 (48565..51708) Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis YAL049C 0 51856 52596 ON 1 YAL049C complement(51856..52596) Cytoplasmic protein of unknown function, potential Hsp82p interactor YAL048C 0 52802 54790 ON 1 GEM1 complement(52802..54790) Evolutionarily-conserved tail-anchored outer mitochondrial membrane GTPase which regulates mitochondrial morphology; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; not required for pheromone-induced cell death YAL047W-A 1 54585 54914 OFF 1 YAL047W-A (54585..54914) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL047C 0 54990 56858 ON 1 SPC72 complement(54990..56858) Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization YAL046C 0 57030 57386 ON 1 YAL046C complement(57030..57386) Putative protein of unknown function; YAL046C is not an essential gene YAL045C 0 57489 57797 OFF 1 YAL045C complement(57489..57797) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A YAL044W-A 1 57519 57851 ON 1 YAL044W-A (57519..57851) Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein YAL044C 0 57951 58463 ON 1 GCV3 complement(57951..58463) H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm YAL043C 0 58696 61053 ON 1 PTA1 complement(58696..61053) Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII YAL042C-A 0 61232 61609 OFF 1 YAL042C-A complement(61232..61609) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene YAL042W 1 61317 62564 ON 1 ERV46 (61317..62564) Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport YAL041W 1 62841 65405 ON 1 CDC24 (62841..65405) Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing YAL040C 0 65779 67521 ON 1 CLN3 complement(65779..67521) G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis YAL039C 0 68717 69526 ON 1 CYC3 complement(68717..69526) Cytochrome c heme lyase (holocytochrome c synthase), attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in the mitochondrial intermembrane space; human ortholog may have a role in microphthalmia with linear skin defects (MLS) YAL038W 1 71787 73289 ON 1 CDC19 (71787..73289) Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration YAL037C-B 0 72327 73301 OFF 1 YAL037C-B complement(72327..73301) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAL037C-A 0 73427 73519 OFF 1 YAL037C-A complement(73427..73519) Putative protein of unknown function YAL037W 1 74021 74824 ON 1 YAL037W (74021..74824) Putative protein of unknown function YAL036C 0 75044 76153 ON 1 RBG1 complement(75044..76153) Member of the DRG family of GTP-binding proteins; interacts with translating ribosomes and with Tma46p YAL035W 1 76428 79436 ON 1 FUN12 (76428..79436) GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 YAL034C-B 0 79490 79843 OFF 1 YAL034C-B complement(79490..79843) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL034W-A 1 79719 80588 ON 1 MTW1 (79719..80588) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly YAL034C 0 80711 81952 ON 1 FUN19 complement(80711..81952) Non-essential protein of unknown function YAL033W 1 82707 83228 ON 1 POP5 (82707..83228) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends YAL032C 0 83336 84475 ON 1 PRP45 complement(83336..84475) Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene YAL031W-A 1 84670 84978 OFF 1 YAL031W-A (84670..84978) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL031C 0 84750 87032 ON 1 GIP4 complement(84750..87032) Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate YAL030W 1 87287 87753 ON 1 SNC1 (87287..87388,87502..87753) Vesicle membrane receptor protein (v-SNARE) involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins YAL029C 0 87856 92271 ON 1 MYO4 complement(87856..92271) One of two type V myosin motors (along with MYO2) involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p YAL028W 1 92901 94487 ON 1 FRT2 (92901..94487) Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike Frt1p), promotes growth in conditions of high Na+, alkaline pH, or cell wall stress; potential Cdc28p substrate YAL027W 1 94688 95473 ON 1 YAL027W (94688..95473) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YAL027W is a non-essential gene YAL026C-A 0 95387 95824 OFF 1 YAL026C-A complement(95387..95824) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAL027W and the verified gene DRS2 YAL026C 0 95631 99698 ON 1 DRS2 complement(95631..99698) Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vessicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease YAL025C 0 100226 101146 ON 1 MAK16 complement(100226..101146) Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus YAL024C 0 101566 105873 ON 1 LTE1 complement(101566..105873) Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP YAL023C 0 106273 108552 ON 1 PMT2 complement(106273..108552) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt1p, can instead interact with Pmt5p in some conditions; target for new antifungals YAL022C 0 108878 110431 ON 1 FUN26 complement(108878..110431) Nucleoside transporter with broad nucleoside selectivity; localized to intracellular membranes YAL021C 0 110847 113360 ON 1 CCR4 complement(110847..113360) Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening YAL020C 0 113615 114616 ON 1 ATS1 complement(113615..114616) Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle YAL019W-A 1 114251 114820 OFF 1 YAL019W-A (114251..114820) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL019W 1 114920 118315 ON 1 FUN30 (114920..118315) Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YAL018C 0 118565 119542 ON 1 YAL018C complement(118565..119542) Putative protein of unknown function YAL017W 1 120226 124296 ON 1 PSK1 (120226..124296) One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status YAL016C-B 0 124308 124493 OFF 1 YAL016C-B complement(124308..124493) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL016C-A 0 124756 125070 OFF 1 YAL016C-A complement(124756..125070) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAL016W 1 124880 126787 ON 1 TPD3 (124880..126787) Regulatory subunit A of the heterotrimeric protein phosphatase 2A, which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and for transcription by RNA polymerase III YAL015C 0 126904 128103 ON 1 NTG1 complement(126904..128103) DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion YAL014C 0 128253 129020 ON 1 SYN8 complement(128253..129020) Endosomal SNARE related to mammalian syntaxin 8 YAL013W 1 129271 130533 ON 1 DEP1 (129271..130533) Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation YAL012W 1 130802 131986 ON 1 CYS3 (130802..131986) Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine YAL011W 1 132202 134079 ON 1 SWC3 (132202..134079) Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae YAL010C 0 134186 135667 ON 1 MDM10 complement(134186..135667) Subunit of both the Mdm10-Mdm12-Mmm1 complex and the mitochondrial sorting and assembly machinery (SAM complex); functions in both the general and Tom40p-specific pathways for import and assembly of outer membrane beta-barrel proteins YAL009W 1 135856 136635 ON 1 SPO7 (135856..136635) Regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic genes; required for normal nuclear envelope morphology and sporulation YAL008W 1 136916 137512 ON 1 FUN14 (136916..137512) Mitochondrial protein of unknown function YAL007C 0 137700 138347 ON 1 ERP2 complement(137700..138347) Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles YAL005C 0 139505 141433 ON 1 SSA1 complement(139505..141433) ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall YAL004W 1 140762 141409 OFF 1 YAL004W (140762..141409) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C YAL003W 1 142176 143162 ON 1 EFB1 (142176..142255,142622..143162) Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site YAL002W 1 143709 147533 ON 1 VPS8 (143709..147533) Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif YAL001C 0 147596 151168 ON 1 TFC3 complement(147596..151008,151099..151168) Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding YAR002W 1 152259 153878 ON 1 NUP60 (152259..153878) Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance YAR002C-A 0 154067 154726 ON 1 ERP1 complement(154067..154726) Protein that forms a heterotrimeric complex with Erp2p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles YAR003W 1 155007 156287 ON 1 SWD1 (155007..156287) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 YAR007C 0 156756 158621 ON 1 RFA1 complement(156756..158621) Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination YAR008W 1 158967 159794 ON 1 SEN34 (158967..159794) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease YAR014C 0 166743 168866 ON 1 BUD14 complement(166743..168866) Protein involved in bud-site selection, Bud14p-Glc7p complex functions as a cortical regulator of dynein; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YAR015W 1 169370 170290 ON 1 ADE1 (169370..170290) N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine YAR018C 0 170391 171698 ON 1 KIN3 complement(170391..171698) Nonessential protein kinase with unknown cellular role YAR019C 0 172209 175133 ON 1 CDC15 complement(172209..175133) Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p YAR019W-A 1 174996 175340 OFF 1 YAR019W-A (174996..175340) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR020C 0 176854 177021 ON 1 PAU7 complement(176854..177021) Part of 23-member seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme YAR023C 0 179279 179818 ON 1 YAR023C complement(179279..179818) Putative integral membrane protein, member of DUP240 gene family YAR027W 1 183764 184471 ON 1 UIP3 (183764..184471) Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family YAR028W 1 184886 185590 ON 1 YAR028W (184886..185590) Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YAR029W 1 186315 186539 ON 1 YAR029W (186315..186539) Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YAR030C 0 186506 186847 OFF 1 YAR030C complement(186506..186847) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 YAR031W 1 186830 187726 ON 1 PRM9 (186830..187726) Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family YAR033W 1 188101 188805 ON 1 MST28 (188101..188805) Putative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles YAR035W 1 190187 192250 ON 1 YAT1 (190187..192250) Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix YAR035C-A 0 192331 192411 OFF 1 YAR035C-A complement(192331..192411) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YAR042W 1 192613 196179 ON 1 SWH1 (192613..196179) Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction YAR047C 0 201460 201780 OFF 1 YAR047C complement(201460..201780) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR050W 1 203394 208007 ON 1 FLO1 (203394..208007) Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p YAR053W 1 208358 208654 OFF 1 YAR053W (208358..208654) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR060C 0 217148 217483 OFF 1 YAR060C complement(217148..217483) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR061W 1 218131 218334 ON 1 YAR061W (218131..218334) Hypothetical protein YAR062W 1 218540 219136 OFF 1 YAR062W (218540..219136) Hypothetical protein YAR064W 1 220189 220488 ON 1 YAR064W (220189..220488) Putative protein of unknown function YAR066W 1 221040 221651 ON 1 YAR066W (221040..221651) Putative GPI protein YAR068W 1 222397 222882 ON 1 YAR068W (222397..222882) Fungal-specific protein of unknown function; induced in respiratory-deficient cells YAR069C 0 224002 224295 OFF 1 YAR069C complement(224002..224295) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR070C 0 224554 224853 OFF 1 YAR070C complement(224554..224853) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YAR071W 1 225451 226854 ON 1 PHO11 (225451..226854) One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 YAR073W 1 227733 228944 ON 1 IMD1 (227733..228944) Nonfunctional protein with homology to IMP dehydrogenase; probable pseudogene, located close to the telomere; is not expressed at detectable levels; YAR073W and YAR075W comprise a continuous reading frame in some strains of S. cerevisiae YAR075W 1 228835 229308 ON 1 YAR075W (228835..229308) Non-functional protein with homology IMP dehydrogenase; YAR073W/IMD1 and YAR075W comprise a continuous reading frame in some strains of S. cerevisiae. YBL113C 0 280 2658 ON 2 YBL113C complement(280..2658) Helicase-like protein encoded within the telomeric Y' element YBL113W-A 1 646 1128 OFF 2 YBL113W-A (646..1128) Dubious open reading frame unlikely to encode a protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBL112C 0 2582 2899 ON 2 YBL112C complement(2582..2899) Putative protein of unknown function; YBL112C is contained within TEL02L YBL111C 0 2907 5009 ON 2 YBL111C complement(2907..4116,4216..5009) Helicase-like protein encoded within the telomeric Y' element YBL109W 1 5790 6125 OFF 2 YBL109W (5790..6125) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBL108C-A 0 7605 7733 ON 2 YBL108C-A complement(7605..7733) Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36. YBL108W 1 8177 8482 OFF 2 YBL108W (8177..8482) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL107W-A 1 9268 9372 OFF 2 YBL107W-A (9268..9372) Similar to TyA and TyB proteins YBL107C 0 9961 10551 ON 2 YBL107C complement(9961..10551) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBL107C is not an essential gene YBL106C 0 10847 13879 ON 2 SRO77 complement(10847..13879) Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p YBL105C 0 14241 17696 ON 2 PKC1 complement(14241..17696) Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) YBL104C 0 18177 21293 ON 2 YBL104C complement(18177..21293) Putative protein of unknown function, promoter contains multiple GCN4 binding sites YBL103C 0 22075 23535 ON 2 RTG3 complement(22075..23535) Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways YBL102W 1 24098 24745 ON 2 SFT2 (24098..24745) Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 YBL101C 0 24946 28299 ON 2 ECM21 complement(24946..28299) Non-essential protein of unknown function; promoter contains several Gcn4p binding elements YBL100W-C 1 28427 28546 OFF 2 YBL100W-C (28427..28546) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBL100C 0 36986 37300 OFF 2 YBL100C complement(36986..37300) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1 YBL099W 1 37050 38687 ON 2 ATP1 (37050..38687) Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YBL098W 1 39142 40524 ON 2 BNA4 (39142..40524) Kynurenine 3-mono oxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway YBL097W 1 40828 43092 ON 2 BRN1 (40828..43092) Essential protein required for chromosome condensation, likely to function as an intrinsic component of the condensation machinery, may influence multiple aspects of chromosome transmission and dynamics YBL096C 0 43171 43479 OFF 2 YBL096C complement(43171..43479) Non-essential protein of unknown function YBL095W 1 43274 44086 ON 2 YBL095W (43274..44086) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBL094C 0 43762 44094 OFF 2 YBL094C complement(43762..44094) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W YBL093C 0 44253 44915 ON 2 ROX3 complement(44253..44915) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme YBL092W 1 45975 46367 ON 2 RPL32 (45975..46367) Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencing YBL091C-A 0 46562 47177 ON 2 SCS22 complement(46562..47055,47144..47177) Protein involved in regulation of phospholipid metabolism; homolog of Scs2p; similar to D. melanogaster inturned protein YBL091C 0 47360 48625 ON 2 MAP2 complement(47360..48625) Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p YBL090W 1 48822 49355 ON 2 MRP21 (48822..49355) Mitochondrial ribosomal protein of the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences YBL089W 1 49571 50950 ON 2 AVT5 (49571..50950) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YBL088C 0 51016 59379 ON 2 TEL1 complement(51016..59379) Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene YBL087C 0 59818 60735 ON 2 RPL23A complement(59818..60189,60694..60735) Protein component of the large (60S) ribosomal subunit, identical to Rpl23Bp and has similarity to E. coli L14 and rat L23 ribosomal proteins YBL086C 0 61199 62599 ON 2 YBL086C complement(61199..62599) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YBL085W 1 63873 66815 ON 2 BOI1 (63873..66815) Protein implicated in polar growth, functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain YBL084C 0 67166 69442 ON 2 CDC27 complement(67166..69442) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YBL083C 0 69710 70135 OFF 2 YBL083C complement(69710..70135) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 YBL082C 0 69748 71124 ON 2 ALG3 complement(69748..71124) Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins YBL081W 1 71863 72969 ON 2 YBL081W (71863..72969) Non-essential protein of unknown function YBL080C 0 73067 74692 ON 2 PET112 complement(73067..74692) Protein required for mitochondrial translation; mutation is functionally complemented by a Bacillus subtilis ortholog YBL079W 1 75256 79764 ON 2 NUP170 (75256..79764) Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p YBL078C 0 80375 80728 ON 2 ATG8 complement(80375..80728) Protein required for autophagy; modified by the serial action of Atg4p, Atg7p, and Atg3p, and conjugated at the C terminus with phosphatidylethanolamine, to become the form essential for generation of autophagosomes YBL077W 1 80895 81326 OFF 2 YBL077W (80895..81326) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C YBL076C 0 81041 84259 ON 2 ILS1 complement(81041..84259) Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A YBL075C 0 84497 86446 ON 2 SSA3 complement(84497..86446) ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm YBL074C 0 86720 87787 ON 2 AAR2 complement(86720..87787) Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron YBL073W 1 87644 87955 OFF 2 YBL073W (87644..87955) Hypothetical protein; open reading frame overlaps 5' end of essential AAR2 gene encoding a component of the U5 snRNP required for splicing of U3 precursors YBL072C 0 88521 89123 ON 2 RPS8A complement(88521..89123) Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein YBL071C-B 0 89456 89554 OFF 2 YBL071C-B complement(89456..89554) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBL071W-A 1 89976 90224 ON 2 KTI11 (89976..90224) Protein required for synthesis of diphthamide, a modified histidine residue of translation elongation factor 2; functions with Dph1p, Dph2p, Jjj3p, and Dph5p; required, with Elongator complex, for modification of wobble nucleosides in tRNA YBL071C 0 90221 90529 OFF 2 YBL071C complement(90221..90529) Dubious open reading frame, predicted protein contains a peroxisomal targeting signal YBL070C 0 90603 90923 OFF 2 YBL070C complement(90603..90923) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL069W 1 90739 92028 ON 2 AST1 (90739..92028) Peripheral membrane protein that interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane, possibly by influencing its incorporation into lipid rafts YBL068W-A 1 91792 92028 OFF 2 YBL068W-A (91792..92028) Dubious open reading frame unlikely to encode a protein; identified by fungal homology and RT-PCR YBL068W 1 92412 93395 ON 2 PRS4 (92412..93395) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YBL067C 0 93641 95884 ON 2 UBP13 complement(93641..95884) Putative ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions YBL066C 0 96671 100117 ON 2 SEF1 complement(96671..100117) Putative transcription factor, has homolog in Kluyveromyces lactis YBL065W 1 99965 100309 OFF 2 YBL065W (99965..100309) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene YBL064C 0 100373 101158 ON 2 PRX1 complement(100373..101158) Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stress YBL063W 1 101888 105223 ON 2 KIP1 (101888..105223) Kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation; functionally redundant with Cin8p YBL062W 1 105310 105690 OFF 2 YBL062W (105310..105690) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL061C 0 105318 107408 ON 2 SKT5 complement(105318..107408) Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation YBL060W 1 107934 109997 ON 2 YBL060W (107934..109997) Non-essential protein proposed to be a nucleotide exchange factor for Arf3 YBL059C-A 0 110127 110541 ON 2 YBL059C-A complement(110127..110422,110508..110541) Putative protein of unknown function; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YBL059W 1 110596 111246 ON 2 YBL059W (110596..110881,110951..111246) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBL058W 1 111439 112710 ON 2 SHP1 (111439..112710) UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate YBL057C 0 112803 113447 ON 2 PTH2 complement(112803..113447) One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth YBL056W 1 113765 115171 ON 2 PTC3 (113765..115171) Type 2C protein phosphatase; dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA checkpoint inactivation YBL055C 0 115576 116832 ON 2 YBL055C complement(115576..116832) 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases YBL054W 1 117592 119169 ON 2 YBL054W (117592..119169) Protein of unknown function involved in rRNA and ribosome biosynthesis YBL053W 1 119338 119712 OFF 2 YBL053W (119338..119712) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL052C 0 119382 121877 ON 2 SAS3 complement(119382..121877) Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality YBL051C 0 122756 124762 ON 2 PIN4 complement(122756..124762) Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage YBL050W 1 125128 126122 ON 2 SEC17 (125128..125157,125274..126122) Peripheral membrane protein required for vesicular transport between ER and Golgi and for the 'priming' step in homotypic vacuole fusion, part of the cis-SNARE complex; has similarity to alpha-SNAP YBL049W 1 126831 127247 ON 2 MOH1 (126831..127247) Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for viability in stationary phase YBL048W 1 127302 127613 OFF 2 YBL048W (127302..127613) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBL047C 0 127898 132043 ON 2 EDE1 complement(127898..132043) Key endocytic protein involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins YBL046W 1 132427 133752 ON 2 PSY4 (132427..133752) Putative regulatory subunit of an evolutionarily conserved protein phosphatase complex containing the catalytic subunit Pph3p and a third subunit Psy2p; required for cisplatin resistance; GFP-fusion protein localizes to the nucleus YBL045C 0 134146 135519 ON 2 COR1 complement(134146..135519) Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain YBL044W 1 136001 136369 OFF 2 YBL044W (136001..136369) Putative protein of unknown function; YBL044W is not an essential protein YBL043W 1 136691 137464 ON 2 ECM13 (136691..137464) Non-essential protein of unknown function YBL042C 0 138344 140263 ON 2 FUI1 complement(138344..140263) High affinity uridine permease, localized to the plasma membrane; not involved in uracil transport YBL041W 1 141250 141975 ON 2 PRE7 (141250..141975) 20S proteasome beta-type subunit YBL040C 0 142115 142871 ON 2 ERD2 complement(142115..142752,142850..142871) HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins YBL039W-B 1 143396 143575 ON 2 YBL039W-B (143396..143575) Putative protein of unknown function YBL039C 0 143992 145731 ON 2 URA7 complement(143992..145731) Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis YBL039C-A 0 144951 145034 OFF 2 YBL039C-A complement(144951..145034) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF URA7; identified by expression profiling and mass spectrometry YBL038W 1 146190 146888 ON 2 MRPL16 (146190..146888) Mitochondrial ribosomal protein of the large subunit YBL037W 1 147212 150289 ON 2 APL3 (147212..150289) Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport YBL036C 0 150450 151223 ON 2 YBL036C complement(150450..151223) Single-domain racemase, possibly non-specific due to the lack of the second domain, which presumably determines specificity; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS YBL035C 0 151496 153613 ON 2 POL12 complement(151496..153613) B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation YBL034C 0 153851 158392 ON 2 STU1 complement(153851..158392) Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles YBL033C 0 158659 159696 ON 2 RIB1 complement(158659..159696) GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway YBL032W 1 160187 161332 ON 2 HEK2 (160187..161332) RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length YBL031W 1 161702 162718 ON 2 SHE1 (161702..162718) Cytoskeletal protein of unknown function; overexpression causes growth arrest YBL030C 0 163044 164000 ON 2 PET9 complement(163044..164000) Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; required for viability in many common lab strains carrying a mutation in the polymorphic SAL1 gene YBL029C-A 0 164491 164775 ON 2 YBL029C-A complement(164491..164775) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica YBL029W 1 166137 167267 ON 2 YBL029W (166137..167267) Non-essential protein of unknown function YBL028C 0 167521 167841 ON 2 YBL028C complement(167521..167841) Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis YBL027W 1 168426 169379 ON 2 RPL19B (168426..168427,168812..169379) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal YBL026W 1 170626 171041 ON 2 LSM2 (170626..170679,170808..171041) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YBL025W 1 171484 171921 ON 2 RRN10 (171484..171921) Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I YBL024W 1 172537 174591 ON 2 NCL1 (172537..174591) S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; similar to Nop2p and human proliferation associated nucleolar protein p120 YBL023C 0 174923 177529 ON 2 MCM2 complement(174923..177529) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex YBL022C 0 177877 181278 ON 2 PIM1 complement(177877..181278) ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria YBL021C 0 181663 182097 ON 2 HAP3 complement(181663..182097) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding YBL020W 1 182404 184128 ON 2 RFT1 (182404..184128) Flippase, essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane; mutation is suppressed by expression human p53 protein YBL019W 1 184356 185918 ON 2 APN2 (184356..185918) Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII YBL018C 0 186001 186477 ON 2 POP8 complement(186001..186355,186431..186477) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends YBL017C 0 186847 191586 ON 2 PEP1 complement(186847..191586) Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments YBL016W 1 192454 193515 ON 2 FUS3 (192454..193515) Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivatd by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its degradation YBL015W 1 194125 195705 ON 2 ACH1 (194125..195705) Acetyl-coA hydrolase, primarily localized to mitochondria; required for acetate utilization and for diploid pseudohyphal growth YBL014C 0 199067 201751 ON 2 RRN6 complement(199067..201751) Protein involved in the transcription of 35S rRNA genes by RNA polymerase I; component of the core factor (CF) complex also composed of Rrn11p, Rrn7p and TATA-binding protein YBL013W 1 202059 203264 ON 2 FMT1 (202059..203264) Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate YBL012C 0 203409 203810 OFF 2 YBL012C complement(203409..203810) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBL011W 1 203541 205820 ON 2 SCT1 (203541..205820) Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed YBL010C 0 206110 206952 ON 2 YBL010C complement(206110..206952) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles YBL009W 1 207197 209227 ON 2 ALK2 (207197..209227) Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk1p and to mammalian haspins YBL008W-A 1 209412 209651 OFF 2 YBL008W-A (209412..209651) Putative protein of unknown function; identified by fungal homology and RT-PCR YBL008W 1 209656 212178 ON 2 HIR1 (209656..212178) Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores YBL007C 0 212635 216369 ON 2 SLA1 complement(212635..216369) Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis YBL006C 0 216590 217132 ON 2 LDB7 complement(216590..217132) Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions YBL006W-A 1 216714 216863 OFF 2 YBL006W-A (216714..216863) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene LDB7/YBL006C YBL005W 1 217473 220403 ON 2 PDR3 (217473..220403) Transcriptional activator of the pleiotropic drug resistance network, regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements) YBL004W 1 227639 235120 ON 2 UTP20 (227639..235120) Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA YBL003C 0 235397 235795 ON 2 HTA2 complement(235397..235795) One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p YBL002W 1 236495 236890 ON 2 HTB2 (236495..236890) One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation YBL001C 0 237155 237469 ON 2 ECM15 complement(237155..237469) Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis YBR001C 0 238943 241285 ON 2 NTH2 complement(238943..241285) Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses YBR002C 0 241710 242570 ON 2 RER2 complement(241710..242570) Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting YBR003W 1 242811 244232 ON 2 COQ1 (242811..244232) Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis YBR004C 0 244368 245669 ON 2 GPI18 complement(244368..245669) Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria YBR005W 1 245908 246549 ON 2 RCR1 (245908..246549) Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane YBR006W 1 247012 248505 ON 2 UGA2 (247012..248505) Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm YBR007C 0 248807 251017 ON 2 DSF2 complement(248807..251017) Deletion suppressor of mpt5 mutation YBR008C 0 252564 254210 ON 2 FLR1 complement(252564..254210) Plasma membrane multidrug transporter of the major facilitator superfamily, involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs YBR009C 0 255371 255682 ON 2 HHF1 complement(255371..255682) One of two identical histone H4 proteins (see also HHF2); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity YBR010W 1 256329 256739 ON 2 HHT1 (256329..256739) One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation YBR011C 0 257110 257973 ON 2 IPP1 complement(257110..257973) Cytoplasmic inorganic pyrophosphatase (PPase), catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Scer_YGOB_YBR013C-YBR012C 0 259145 265879 ON 2 YBR013C-YBR012C complement(259145..259575,265498..265879) ANNOTATED BY YGOB - Gene is interrupted by a Ty element in S288c but not YJM789. YBR012C 0 259145 259564 OFF 2 YBR012C complement(259145..259564) Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p YBR013C 0 265490 265879 OFF 2 YBR013C complement(265490..265879) Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein YBR014C 0 266725 267336 ON 2 YBR014C complement(266725..267336) Putative membrane-localized protein of unknown function, contains glutaredoxin domain YBR015C 0 267710 269503 ON 2 MNN2 complement(267710..269503) Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment YBR016W 1 270247 270633 ON 2 YBR016W (270247..270633) Plasma membrane protein of unknown function; has similarity to hydrophilins, which are hydrophilic, glycine-rich proteins involved in the adaptive response to hyperosmotic conditions YBR017C 0 270947 273703 ON 2 KAP104 complement(270947..273703) Transportin, cytosolic karyopherin beta 2 involved in delivery of heterogeneous nuclear ribonucleoproteins to the nucleoplasm, binds rg-nuclear localization signals on Nab2p and Hrp1p, plays a role in cell-cycle progression YBR018C 0 274427 275527 ON 2 GAL7 complement(274427..275527) Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism YBR019C 0 276253 278352 ON 2 GAL10 complement(276253..278352) UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers YBR020W 1 279021 280607 ON 2 GAL1 (279021..280607) Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p YBR021W 1 281443 283344 ON 2 FUR4 (281443..283344) Uracil permease, localized to the plasma membrane; expression is tightly regulated by uracil levels and environmental cues YBR022W 1 283738 284271 ON 2 POA1 (283738..284271) Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing YBR023C 0 284428 287925 ON 2 CHS3 complement(284428..287925) Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan YBR024W 1 289445 290350 ON 2 SCO2 (289445..290350) Protein anchored to the mitochondrial inner membrane, similar to Sco1p and may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p YBR025C 0 290681 291865 ON 2 OLA1 complement(290681..291865) P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein levels are induced by hydrogen peroxide YBR026C 0 292877 294019 ON 2 ETR1 complement(292877..294019) 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis YBR027C 0 294024 294356 OFF 2 YBR027C complement(294024..294356) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR028C 0 294425 296002 ON 2 YBR028C complement(294425..296002) Putative protein kinase, possible substrate of cAMP-dependent protein kinase (PKA) YBR029C 0 296369 297742 ON 2 CDS1 complement(296369..297742) Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids YBR030W 1 298292 299950 ON 2 YBR030W (298292..299950) Putative protein of unknown function; predicted protein contains a SET domain (S-adenosyl-L-methionine-binding fold) YBR031W 1 300166 301254 ON 2 RPL4A (300166..301254) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins YBR032W 1 301519 301821 OFF 2 YBR032W (301519..301821) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YBR033W 1 301944 304703 ON 2 EDS1 (301944..304703) Putative zinc cluster protein; YBR033W is not an essential gene YBR034C 0 304930 305976 ON 2 HMT1 complement(304930..305976) Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, thus facilitating nuclear export of these proteins; required for viability of npl3 mutants YBR035C 0 306269 306955 ON 2 PDX3 complement(306269..306955) Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism YBR036C 0 309081 310313 ON 2 CSG2 complement(309081..310313) Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations YBR037C 0 310564 311451 ON 2 SCO1 complement(310564..311451) Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; has similarity to thioredoxins YBR038W 1 311897 314788 ON 2 CHS2 (311897..314788) Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for the synthesis of chitin in the primary septum during cytokinesis YBR039W 1 315575 316510 ON 2 ATP3 (315575..316510) Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YBR040W 1 316968 317864 ON 2 FIG1 (316968..317864) Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating YBR041W 1 318266 320275 ON 2 FAT1 (318266..320275) Fatty acid transporter and very long-chain fatty acyl-CoA synthetase, may form a complex with Faa1p or Faa4p that imports and activates exogenous fatty acids YBR042C 0 320416 321609 ON 2 CST26 complement(320416..321609) Protein of unknown function, affects chromosome stability when overexpressed YBR043C 0 321876 323945 ON 2 QDR3 complement(321876..323945) Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin YBR044C 0 324337 326058 ON 2 TCM62 complement(324337..326058) Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone YBR045C 0 328369 330090 ON 2 GIP1 complement(328369..330090) Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p YBR046C 0 330505 331509 ON 2 ZTA1 complement(330505..331509) Zeta-crystallin homolog, found in the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin, which has quinone oxidoreductase activity YBR047W 1 331831 332358 ON 2 YBR047W (331831..332358) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR048W 1 332829 333810 ON 2 RPS11B (332829..332873,333385..333810) Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins YBR049C 0 334384 336816 ON 2 REB1 complement(334384..336816) RNA polymerase I enhancer binding protein; DNA binding protein which binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription YBR050C 0 337181 338197 ON 2 REG2 complement(337181..338197) Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease YBR051W 1 337986 338336 OFF 2 YBR051W (337986..338336) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase YBR052C 0 338718 339350 ON 2 RFS1 complement(338718..339350) Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YBR053C 0 339673 340749 ON 2 YBR053C complement(339673..340749) Putative protein of unknown function; induced by cell wall perturbation YBR054W 1 343099 344133 ON 2 YRO2 (343099..344133) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p YBR055C 0 344600 347299 ON 2 PRP6 complement(344600..347299) Splicing factor, component of the U4/U6-U5 snRNP complex YBR056W 1 347877 349382 ON 2 YBR056W (347877..349382) Putative cytoplasmic protein of unknown function YBR056W-A 1 351253 351453 ON 2 YBR056W-A (351253..351453) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YBR056C-B YBR056C-B 0 351291 351449 OFF 2 YBR056C-B complement(351291..351449) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the dubious ORF YBR056W-A YBR057C 0 352191 353291 ON 2 MUM2 complement(352191..353291) Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex YBR058C 0 353670 356015 ON 2 UBP14 complement(353670..356015) Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T YBR058C-A 0 356322 356564 ON 2 TSC3 complement(356322..356564) Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis YBR059C 0 356859 360185 ON 2 AKL1 complement(356859..360185) Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization YBR060C 0 360650 362512 ON 2 ORC2 complement(360650..362512) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p YBR061C 0 364785 365717 ON 2 TRM7 complement(364785..365717) 2'-O-ribose methyltransferase, methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop YBR062C 0 365974 366598 ON 2 YBR062C complement(365974..366500,366583..366598) Hypothetical protein YBR063C 0 366968 368182 ON 2 YBR063C complement(366968..368182) Putative protein of unknown function; YBR063C is not an essential gene YBR064W 1 367761 368189 OFF 2 YBR064W (367761..368189) Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C YBR065C 0 368582 369676 ON 2 ECM2 complement(368582..369676) Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p YBR066C 0 370035 370697 ON 2 NRG2 complement(370035..370697) Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p YBR067C 0 372100 372732 ON 2 TIP1 complement(372100..372732) Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins YBR068C 0 373858 375687 ON 2 BAP2 complement(373858..375687) High-affinity leucine permease, functions as a branched-chain amino acid permease involved in the uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains YBR069C 0 376571 378430 ON 2 TAT1 complement(376571..378430) Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 resistance YBR070C 0 379218 379931 ON 2 ALG14 complement(379218..379931) Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases YBR071W 1 380408 381043 ON 2 YBR071W (380408..381043) Putative protein of unknown function; (GFP)-fusion and epitope-tagged proteins localize to the cytoplasm; mRNA expression may be regulated by the cell cycle and/or cell wall stress YBR072W 1 382027 382671 ON 2 HSP26 (382027..382671) Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires a heat-induced conformational change; not expressed in unstressed cells YBR072C-A 0 382855 383016 OFF 2 YBR072C-A complement(382855..383016) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR073W 1 383209 385983 ON 2 RDH54 (383209..385983) DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during mitosis and meiosis; proposed to be involved in crossover interference YBR074W 1 386281 389211 ON 2 YBR074W (386281..389211) Putative metalloprotease YBR076W 1 390369 391388 ON 2 ECM8 (390369..391388) Non-essential protein of unknown function YBR076C-A 0 391345 391611 OFF 2 YBR076C-A complement(391345..391611) Dubious open reading frame unlikely to encode a protein; partially overlaps verified gene ECM8; identified by fungal homology and RT-PCR YBR077C 0 391799 392287 ON 2 SLM4 complement(391799..392287) Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 YBR078W 1 393118 394854 ON 2 ECM33 (393118..393175,393506..394854) GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; similar to Sps2p and Pst1p YBR079C 0 395377 398271 ON 2 RPG1 complement(395377..398271) Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes YBR080C 0 398608 400884 ON 2 SEC18 complement(398608..400884) ATPase required for the release of Sec17p during the 'priming' step in homotypic vacuole fusion and for ER to Golgi transport; homolog of the mammalian NSF YBR081C 0 401247 405245 ON 2 SPT7 complement(401247..405245) Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex YBR082C 0 406622 407163 ON 2 UBC4 complement(406622..407021,407117..407163) Ubiquitin-conjugating enzyme (E2), mediates degradation of short-lived and abnormal proteins; interacts with E3-CaM in ubiquitinating calmodulin; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response YBR083W 1 409163 410623 ON 2 TEC1 (409163..410623) Transcription factor required for full Ty1 epxression, Ty1-mediated gene activation, and haploid invasive and diploid pseudohyphal growth; TEA/ATTS DNA-binding domain family member YBR084W 1 411048 413975 ON 2 MIS1 (411048..413975) Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase YBR084C-A 0 414180 415255 ON 2 RPL19A complement(414180..414747,415254..415255) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Bp and has similarity to rat L19 ribosomal protein; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal YBR085W 1 415977 416900 ON 2 AAC3 (415977..416900) Mitochondrial inner membrane ADP/ATP translocator, exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Pet9p and Aac1p; has roles in maintenance of viability and in respiration YBR085C-A 0 418901 419158 ON 2 YBR085C-A complement(418901..419158) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus YBR086C 0 420195 423035 ON 2 IST2 complement(420195..423035) Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process YBR087W 1 423759 424823 ON 2 RFC5 (423759..424823) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YBR088C 0 424984 425760 ON 2 POL30 complement(424984..425760) Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair YBR089W 1 425177 425776 OFF 2 YBR089W (425177..425776) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 YBR089C-A 0 426184 426483 ON 2 NHP6B complement(426184..426483) High-mobility group non-histone chromatin protein, functionally redundant with Nhp6Ap; homologous to mammalian high mobility group proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain promoters YBR090C 0 426327 427052 OFF 2 YBR090C complement(426327..426510,426868..427052) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YBR091C 0 427149 427478 ON 2 MRS5 complement(427149..427478) Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins YBR092C 0 427692 429095 ON 2 PHO3 complement(427692..429095) Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin YBR093C 0 429542 430945 ON 2 PHO5 complement(429542..430945) Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 YBR094W 1 432030 434291 ON 2 PBY1 (432030..434291) Putative tubulin tyrosine ligase associated with P-bodies YBR095C 0 434400 435692 ON 2 RXT2 complement(434400..435692) Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth YBR096W 1 436015 436707 ON 2 YBR096W (436015..436707) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER YBR097W 1 436945 441309 ON 2 VPS15 (436945..441309) Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p YBR098W 1 441509 443584 ON 2 MMS4 (441509..443584) Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair YBR099C 0 442918 443301 OFF 2 YBR099C complement(442918..443301) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 YBR101C 0 443815 444687 ON 2 FES1 complement(443815..444687) Hsp70 (Ssa1p) nucleotide exchange factor, cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum YBR102C 0 445056 447317 ON 2 EXO84 complement(445056..447317) Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis YBR103W 1 447703 449310 ON 2 SIF2 (447703..449310) WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus YBR103C-A 0 449314 449457 OFF 2 YBR103C-A complement(449314..449457) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; identified by homology to related yeast YBR104W 1 449661 450650 ON 2 YMC2 (449661..450650) Mitochondrial protein, putative inner membrane transporter with a role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; has similarity with Ymc1p YBR105C 0 450875 451963 ON 2 VID24 complement(450875..451963) Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; involved in proteasome-dependent catabolite degradation of FBPase YBR106W 1 452652 453218 ON 2 PHO88 (452652..453218) Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations YBR107C 0 453787 454524 ON 2 IML3 complement(453787..454524) Protein with a role in kinetochore function, localizes to the outer kinetochore in a Ctf19p-dependent manner, interacts with Chl4p and Ctf19p YBR108W 1 454816 457659 ON 2 YBR108W (454816..457659) Protein interacting with Rsv167p YBR109C 0 457913 458356 ON 2 CMD1 complement(457913..458356) Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin YBR109W-A 1 458602 458826 OFF 2 YBR109W-A (458602..458826) Putative protein of unknown function; questionable ORF from MIPS YBR110W 1 458866 460215 ON 2 ALG1 (458866..460215) Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog YBR111C 0 461172 461867 ON 2 YSA1 complement(461172..461867) Nudix hydrolase family member with ADP-ribose pyrophosphatase activity YBR111W-A 1 462133 462573 ON 2 SUS1 (462133..462203,462284..462423,462494..462573) Protein involved in mRNA export coupled transcription activation; component of the SAGA histone acetylase complex YBR112C 0 462864 465764 ON 2 CYC8 complement(462864..465764) General transcriptional co-repressor, acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters YBR113W 1 465560 466042 OFF 2 YBR113W (465560..466042) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 YBR114W 1 467242 469614 ON 2 RAD16 (467242..469614) Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex YBR115C 0 469742 473920 ON 2 LYS2 complement(469742..473920) Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p YBR116C 0 474193 474720 OFF 2 YBR116C complement(474193..474720) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 YBR117C 0 474386 476431 ON 2 TKL2 complement(474386..476431) Transketolase, similar to Tkl1p; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids YBR118W 1 477665 479041 ON 2 TEF2 (477665..479041) Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes YBR119W 1 479332 480317 ON 2 MUD1 (479332..479339,479429..480317) U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing YBR120C 0 480429 480917 ON 2 CBP6 complement(480429..480917) Mitochondrial translational activator of the COB mRNA YBR121C 0 481358 483361 ON 2 GRS1 complement(481358..483361) Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation YBR121C-A 0 482320 482478 OFF 2 YBR121C-A complement(482320..482478) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely contianed within the verified gene GRS1; identified by expression profiling and mass spectrometry YBR122C 0 483964 484497 ON 2 MRPL36 complement(483964..484497) Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region YBR123C 0 484736 486685 ON 2 TFC1 complement(484736..486685) One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 YBR124W 1 486501 486860 OFF 2 YBR124W (486501..486860) Putative protein of unknown function YBR125C 0 487193 488374 ON 2 PTC4 complement(487193..488374) Cytoplasmic type 2C protein phosphatase; identified as a high-copy number suppressor of the synthetic lethality of a cnb1 mpk1 double deletion; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity YBR126C 0 488899 490386 ON 2 TPS1 complement(488899..490386) Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway YBR126W-A 1 490844 491050 OFF 2 YBR126W-A (490844..491050) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YBR126W-B; identified by gene-trapping, microarray analysis, and genome-wide homology searches YBR126W-B 1 490921 491064 OFF 2 YBR126W-B (490921..491064) Identified by SAGE YBR127C 0 491263 492816 ON 2 VMA2 complement(491263..492816) Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm YBR128C 0 493075 494109 ON 2 ATG14 complement(493075..494109) Subunit of an autophagy-specific phosphatidylinositol 3-kinase complex (with Vps34p, Vps15p, and Vps30p) required for organization of a pre-autophagosomal structure; ATG14 transcription is activated by Gln3p during nitrogen starvation YBR129C 0 494347 495333 ON 2 OPY1 complement(494347..495333) Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR130C 0 495586 496863 ON 2 SHE3 complement(495586..496863) Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance YBR131W 1 497157 499271 ON 2 CCZ1 (497157..499271) Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway YBR131C-A 0 497644 497733 OFF 2 YBR131C-A complement(497644..497733) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR131W. Identified by gene-trapping, microarray analysis, and genome-wide homology searches YBR132C 0 499646 501436 ON 2 AGP2 complement(499646..501436) High affinity polyamine permease, preferentially uses spermidine over putrescine; expression is down-regulated by osmotic stress; plasma membrane carnitine transporter, also functions as a low-affinity amino acid permease YBR133C 0 501798 504281 ON 2 HSL7 complement(501798..504281) Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p YBR134W 1 504237 504638 OFF 2 YBR134W (504237..504638) Dubious open reading frame, unlikely to encode a functional protein; based on available experimental and comparative sequence data YBR135W 1 504848 505300 ON 2 CKS1 (504848..505300) Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters YBR136W 1 505662 512768 ON 2 MEC1 (505662..512768) Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination YBR137W 1 513038 513577 ON 2 YBR137W (513038..513577) Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); YBR137W is not an essential gene YBR138C 0 513756 515330 ON 2 YBR138C complement(513756..515330) Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene YBR139W 1 515658 517184 ON 2 YBR139W (515658..517184) Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner YBR140C 0 517344 526622 ON 2 IRA1 complement(517344..526622) GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase YBR141C 0 527019 528032 ON 2 YBR141C complement(527019..528032) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene YBR141W-A 1 527161 527256 OFF 2 YBR141W-A (527161..527256) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YBR141C; identified by gene-trapping, microarray analysis, and genome-wide homology searching YBR142W 1 528311 530632 ON 2 MAK5 (528311..530632) Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits YBR143C 0 530863 532176 ON 2 SUP45 complement(530863..532176) Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor YBR144C 0 533229 533543 OFF 2 YBR144C complement(533229..533543) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene YBR145W 1 533756 534811 ON 2 ADH5 (533756..534811) Alcohol dehydrogenase isoenzyme V; involved in ethanol production YBR146W 1 535254 536090 ON 2 MRPS9 (535254..536090) Mitochondrial ribosomal protein of the small subunit YBR147W 1 536569 537459 ON 2 YBR147W (536569..537459) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays fluconazole resistance YBR148W 1 537870 539699 ON 2 YSW1 (537870..539699) Protein expressed specifically in spores YBR149W 1 539981 541015 ON 2 ARA1 (539981..541015) NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product YBR150C 0 541203 544487 ON 2 TBS1 complement(541203..544487) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR151W 1 545022 545972 ON 2 APD1 (545022..545972) Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus YBR152W 1 546370 547245 ON 2 SPP381 (546370..547245) mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p YBR153W 1 547454 548188 ON 2 RIB7 (547454..548188) Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway YBR154C 0 548356 549003 ON 2 RPB5 complement(548356..549003) RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation YBR155W 1 549765 550922 ON 2 CNS1 (549765..550922) TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion YBR156C 0 551098 553194 ON 2 SLI15 complement(551098..553194) Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase YBR157C 0 553537 554304 ON 2 ICS2 complement(553537..554304) Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization YBR158W 1 556543 558192 ON 2 AMN1 (556543..558192) Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) YBR159W 1 558679 559722 ON 2 YBR159W (558679..559722) Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides YBR160W 1 560072 560968 ON 2 CDC28 (560072..560968) Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates YBR161W 1 561629 562759 ON 2 CSH1 (561629..562759) Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p YBR162C 0 563198 564565 ON 2 TOS1 complement(563198..564565) Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C YBR162W-A 1 565226 565423 ON 2 YSY6 (565226..565423) Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion YBR163W 1 565718 567475 ON 2 DEM1 (565718..567475) Protein of unknown function, shows similarity to RNA-processing protein Pta1p YBR164C 0 567870 568421 ON 2 ARL1 complement(567870..568421) Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor YBR165W 1 568847 569680 ON 2 UBS1 (568847..569680) Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity YBR166C 0 569837 571195 ON 2 TYR1 complement(569837..571195) Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels YBR167C 0 571463 571885 ON 2 POP7 complement(571463..571885) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends YBR168W 1 572366 573607 ON 2 PEX32 (572366..573607) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p YBR169C 0 573910 575991 ON 2 SSE2 complement(573910..575991) Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; highly homologous to the heat shock protein Sse1p YBR170C 0 576339 578081 ON 2 NPL4 complement(576339..578081) Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation YBR171W 1 578359 578979 ON 2 SEC66 (578359..578979) Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YBR172C 0 579145 581367 ON 2 SMY2 complement(579145..581367) Protein of unknown function that interacts with Myo2p; has similarity to S. pombe Mpd2 YBR173C 0 581721 582167 ON 2 UMP1 complement(581721..582167) Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly YBR174C 0 582333 582647 OFF 2 YBR174C complement(582333..582647) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective YBR175W 1 582403 583350 ON 2 SWD3 (582403..583350) Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 YBR176W 1 583715 584653 ON 2 ECM31 (583715..584653) Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate YBR177C 0 584802 586157 ON 2 EHT1 complement(584802..586157) Acyl-coenzymeA:ethanol O-acyltransferase that plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; contains esterase activity; localizes to lipid particles and the mitochondrial outer membrane YBR178W 1 586066 586440 OFF 2 YBR178W (586066..586440) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C YBR179C 0 586542 589109 ON 2 FZO1 complement(586542..589109) Mitochondrial integral membrane protein involved in mitochondrial fusion and maintenance of the mitochondrial genome; contains N-terminal GTPase domain YBR180W 1 589736 591454 ON 2 DTR1 (589736..591454) Multidrug resistance dityrosine transporter of the major facilitator superfamily, essential for spore wall synthesis, facilitates the translocation of bisformyl dityrosine through the prospore membrane YBR181C 0 591707 592769 ON 2 RPS6B complement(591707..592411,592764..592769) Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein YBR182C 0 593501 594859 ON 2 SMP1 complement(593501..594859) Putative transcription factor involved in regulating the response to osmotic stress; member of the MADS-box family of transcription factors YBR182C-A 0 595356 595550 OFF 2 YBR182C-A complement(595356..595550) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR183W 1 596110 597060 ON 2 YPC1 (596110..597060) Alkaline ceramidase that also has reverse (CoA-independent) ceramide synthase activity, catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance YBR184W 1 597358 598929 ON 2 YBR184W (597358..598929) Putative protein of unknown function; YBR184W is not an essential gene YBR185C 0 599118 599954 ON 2 MBA1 complement(599118..599954) Protein involved in assembly of mitochondrial respiratory complexes; may act as a receptor for proteins destined for export from the mitochondrial matrix to the inner membrane YBR186W 1 600548 602355 ON 2 PCH2 (600548..602098,602212..602355) Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA YBR187W 1 602629 603471 ON 2 GDT1 (602629..603471) Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis YBR188C 0 603680 604102 ON 2 NTC20 complement(603680..604102) Member of a complex, including Prp19p, that binds to the spliceosome; required for pre-mRNA splicing YBR189W 1 604503 605503 ON 2 RPS9B (604503..604509,604923..605503) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps9Ap and has similarity to E. coli S4 and rat S9 ribosomal proteins YBR190W 1 605961 606272 OFF 2 YBR190W (605961..606272) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W YBR191W 1 606265 607135 ON 2 RPL21A (606265..606275,606664..607135) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein YBR191W-A 1 607144 607218 OFF 2 YBR191W-A (607144..607218) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR192W 1 607647 608780 ON 2 RIM2 (607647..608780) Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family YBR193C 0 609077 609748 ON 2 MED8 complement(609077..609748) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YBR194W 1 610033 610404 ON 2 YBR194W (610033..610404) Protein proposed to be associated with the nuclear pore complex YBR195C 0 610609 611877 ON 2 MSI1 complement(610609..611877) Subunit of chromatin assembly factor I (CAF-1), negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase; localizes to the nucleus and cytoplasm; homologous to human retinoblastoma binding proteins RbAp48 and RbAp46 YBR196C 0 612231 613895 ON 2 PGI1 complement(612231..613895) Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation YBR196C-A 0 614019 614168 OFF 2 YBR196C-A complement(614019..614168) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR196C-B 0 614521 614625 OFF 2 YBR196C-B complement(614521..614625) Putative protein of unknown function; identified by expression profiling and mass spectrometry YBR197C 0 615198 615851 ON 2 YBR197C complement(615198..615851) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene YBR198C 0 616122 618518 ON 2 TAF5 complement(616122..618518) Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification YBR199W 1 618904 620298 ON 2 KTR4 (618904..620298) Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YBR200W 1 620867 622522 ON 2 BEM1 (620867..622522) Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p YBR200W-A 1 622978 623142 OFF 2 YBR200W-A (622978..623142) Putative protein of unknown function; identified by fungal homology and RT-PCR YBR201W 1 623572 624207 ON 2 DER1 (623572..624207) Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p YBR201C-A 0 624489 624692 ON 2 YBR201C-A complement(624489..624692) Putative protein of unknown function YBR202W 1 625767 628304 ON 2 CDC47 (625767..628304) Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase YBR203W 1 629163 631937 ON 2 COS111 (629163..631937) Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR204C 0 632249 633376 ON 2 YBR204C complement(632249..633376) Serine hydrolase; YBR204C is not an essential gene YBR205W 1 633617 634831 ON 2 KTR3 (633617..634831) Putative alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YBR206W 1 634596 634919 OFF 2 YBR206W (634596..634919) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 YBR207W 1 635141 636538 ON 2 FTH1 (635141..636538) Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis YBR208C 0 636698 642205 ON 2 DUR1,2 complement(636698..642205) Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YBR209W 1 642578 642895 OFF 2 YBR209W (642578..642895) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR209W is not an essential gene YBR210W 1 645545 645973 ON 2 ERV15 (645545..645973) Protein involved in export of proteins from the endoplasmic reticulum, has similarity to Erv14p YBR211C 0 646153 647127 ON 2 AME1 complement(646153..647127) Essential kinetochore protein associated with microtubules and spindle pole bodies; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance YBR212W 1 647881 649899 ON 2 NGR1 (647881..649899) RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; expressed in stationary phase YBR213W 1 650363 651187 ON 2 MET8 (650363..651187) Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis YBR214W 1 651410 652993 ON 2 SDS24 (651410..652993) One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis YBR215W 1 653351 655312 ON 2 HPC2 (653351..653363,653448..655312) Highly charged, basic protein required for normal cell-cycle regulation of histone gene transcription; mutants display strong synthetic defects with subunits of FACT, a complex that allows RNA Pol II to elongate through nucleosomes YBR216C 0 655571 657595 ON 2 YBP1 complement(655571..657595) Protein required for oxidation of specific cysteine residues of the transcription factor Yap1p, resulting in the nuclear localization of Yap1p in response to stress YBR217W 1 657827 658387 ON 2 ATG12 (657827..658387) Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy YBR218C 0 658702 662244 ON 2 PYC2 complement(658702..662244) Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc1p but differentially regulated; mutations in the human homolog are associated with lactic acidosis YBR219C 0 662494 663298 OFF 2 YBR219C complement(662494..662576,662998..663298) Putative protein of unknown function; YBR219C is not an essential gene YBR220C 0 662990 664672 ON 2 YBR220C complement(662990..664672) Putative protein of unknown function; YBR220C is not an essential gene YBR221C 0 665148 666248 ON 2 PDB1 complement(665148..666248) E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria YBR221W-A 1 666533 666637 OFF 2 YBR221W-A (666533..666637) Putative protein of unknown function; identified by expression profiling and mass spectrometry YBR222C 0 666715 668346 ON 2 PCS60 complement(666715..668346) Peroxisomal AMP-binding protein, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase YBR223C 0 668658 670292 ON 2 TDP1 complement(668658..670292) Tyrosyl-DNA Phosphodiesterase I, hydrolyzes 3'-phosphotyrosyl bonds to generate 3'-phosphate DNA and tyrosine, involved in the repair of DNA lesions created by topoisomerase I YBR223W-A 1 668716 668835 OFF 2 YBR223W-A (668716..668835) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps YBR223C; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR224W 1 670120 670635 OFF 2 YBR224W (670120..670635) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 YBR225W 1 670622 673324 ON 2 YBR225W (670622..673324) Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components YBR226C 0 673150 673560 OFF 2 YBR226C complement(673150..673560) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W YBR227C 0 673567 675129 ON 2 MCX1 complement(673567..675129) Mitochondrial ATP-binding protein, possibly a mitochondrial chaperone with non-proteolytic function; similar to bacterial ClpX proteins YBR228W 1 675308 676222 ON 2 SLX1 (675308..676222) Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p YBR229C 0 676352 679216 ON 2 ROT2 complement(676352..679216) Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations YBR230C 0 679544 680045 ON 2 OM14 complement(679544..679937,680035..680045) Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron YBR230W-A 1 680357 680557 OFF 2 YBR230W-A (680357..680557) Putative protein of unknown function YBR231C 0 682174 683085 ON 2 SWC5 complement(682174..683085) Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YBR232C 0 683368 683727 OFF 2 YBR232C complement(683368..683727) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YBR233W 1 683423 684664 ON 2 PBP2 (683423..684664) RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length YBR233W-A 1 684972 685256 ON 2 DAD3 (684972..685256) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YBR234C 0 685433 686587 ON 2 ARC40 complement(685433..686587) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YBR235W 1 686896 690258 ON 2 YBR235W (686896..690258) Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; YBR235W is not an essential gene YBR236C 0 690378 691688 ON 2 ABD1 complement(690378..691688) Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA YBR237W 1 691964 694513 ON 2 PRP5 (691964..694513) RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA YBR238C 0 695102 697297 ON 2 YBR238C complement(695102..697297) Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span YBR239C 0 698349 699938 ON 2 YBR239C complement(698349..699938) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR239C is not an essential gene YBR240C 0 700485 701837 ON 2 THI2 complement(700485..701837) Zinc finger protein of the Zn(II)2Cys6 type, probable transcriptional activator of thiamine biosynthetic genes YBR241C 0 702584 704050 ON 2 YBR241C complement(702584..704050) Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene YBR242W 1 704665 705381 ON 2 YBR242W (704665..705381) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene YBR243C 0 705442 706788 ON 2 ALG7 complement(705442..706788) UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin YBR244W 1 707523 708011 ON 2 GPX2 (707523..708011) Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress YBR245C 0 708145 711534 ON 2 ISW1 complement(708145..711534) Member of the imitation-switch (ISWI) class of ATP-dependent chromatin remodeling complexes; ATPase that forms a complex with Ioc2p and Ioc4p to regulate transcription elongation, and a complex with Ioc3p to repress transcription initiation YBR246W 1 711586 712749 ON 2 YBR246W (711586..712749) Putative protein of unknown function; deletion results in a weak carboxypeptidase Y missorting/secretion phenotype; YBR246W is not an essential gene YBR247C 0 712999 714450 ON 2 ENP1 complement(712999..714450) Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus YBR248C 0 714802 716460 ON 2 HIS7 complement(714802..716460) Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor YBR249C 0 716877 717989 ON 2 ARO4 complement(716877..717989) 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan YBR250W 1 719028 720599 ON 2 SPO23 (719028..720599) Protein of unknown function; associates with meiosis-specific protein Spo1p YBR251W 1 721385 722308 ON 2 MRPS5 (721385..722308) Mitochondrial ribosomal protein of the small subunit YBR252W 1 722606 723049 ON 2 DUT1 (722606..723049) dUTPase, catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability YBR253W 1 723265 723630 ON 2 SRB6 (723265..723630) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YBR254C 0 723731 724258 ON 2 TRS20 complement(723731..724258) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder YBR255W 1 724451 726535 ON 2 YBR255W (724451..726535) Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YBR255C-A 0 726613 727069 ON 2 YBR255C-A complement(726613..726912,727007..727069) Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species YBR256C 0 727381 728097 ON 2 RIB5 complement(727381..728097) Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway YBR257W 1 728880 729719 ON 2 POP4 (728880..729719) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P YBR258C 0 729729 730157 ON 2 SHG1 complement(729729..730157) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres YBR259W 1 730382 732448 ON 2 YBR259W (730382..732448) Putative protein of unknown function; YBR259W is not an essential gene YBR260C 0 732634 734634 ON 2 RGD1 complement(732634..734634) GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization YBR261C 0 734827 735525 ON 2 YBR261C complement(734827..735525) Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR261C is not an essential gene YBR262C 0 735715 736035 ON 2 YBR262C complement(735715..736035) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR263W 1 736259 737731 ON 2 SHM1 (736259..737731) Mitochondrial serine hydroxymethyltransferase, involved in one-carbon metabolism YBR264C 0 737765 738364 ON 2 YPT10 complement(737765..738364) GTP binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles YBR265W 1 738577 739539 ON 2 TSC10 (738577..739539) 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family YBR267W 1 739836 741017 ON 2 REI1 (739836..741017) Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network YBR266C 0 739931 740383 OFF 2 SLM6 complement(739931..740383) Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase YBR268W 1 741294 741611 ON 2 MRPL37 (741294..741611) Mitochondrial ribosomal protein of the large subunit YBR269C 0 742155 742571 ON 2 YBR269C complement(742155..742571) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YBR270C 0 742756 744393 ON 2 YBR270C complement(742756..744393) Hypothetical protein YBR271W 1 744847 746106 ON 2 YBR271W (744847..746106) Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YBR271W is not as essential gene YBR272C 0 746356 747798 ON 2 HSM3 complement(746356..747798) Protein of unknown function, involved in DNA mismatch repair during slow growth; has weak similarity to Msh1p YBR273C 0 748056 749366 ON 2 UBX7 complement(748056..749366) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p YBR274W 1 749589 751172 ON 2 CHK1 (749589..751172) Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase YBR275C 0 751351 757101 ON 2 RIF1 complement(751351..757101) Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation YBR276C 0 757616 760039 ON 2 PPS1 complement(757616..760039) Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle YBR277C 0 760211 760612 OFF 2 YBR277C complement(760211..760612) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W YBR278W 1 760290 760895 ON 2 DPB3 (760290..760895) Third-largest subunit of DNA polymerase II (DNA polymerase epsilon), required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; mRNA abundance peaks at the G1/S boundary of the cell cycle YBR279W 1 761253 762590 ON 2 PAF1 (761253..762590) RNAP II-associated protein; defines large complex biochemically and functionally distinct from the Srb-Mediator form of Pol II holoenzyme; required for full expression of a subset of cell cycle-regulated genes; homolog of human PD2/hPAF1 YBR280C 0 762780 764693 ON 2 SAF1 complement(762780..764693) F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 YBR281C 0 764966 767602 ON 2 DUG2 complement(764966..767602) Probable di- and tri-peptidase; forms a complex with Dug1p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) YBR282W 1 768236 768676 ON 2 MRPL27 (768236..768676) Mitochondrial ribosomal protein of the large subunit YBR283C 0 768939 770411 ON 2 SSH1 complement(768939..770411) Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential YBR284W 1 771235 773628 ON 2 YBR284W (771235..773628) Putative protein of unknown function; YBR284W is not an essential gene; null mutant exhibits decreased resistance to rapamycin and wortmannin YBR285W 1 773918 774352 ON 2 YBR285W (773918..774352) Putative protein of unknown function; YBR285W is not an essential gene and deletion of YBR285W leads to poor growth on glucose-minimal medium at 15C YBR286W 1 774696 776309 ON 2 APE3 (774696..776309) Vacuolar aminopeptidase Y, processed to mature form by Prb1p YBR287W 1 776567 777850 ON 2 YBR287W (776567..777850) Protein of unknown function; mutation results in a zinc sensitive phenotype YBR288C 0 778008 779459 ON 2 APM3 complement(778008..779459) Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway YBR289W 1 779663 782380 ON 2 SNF5 (779663..782380) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p YBR290W 1 782587 783552 ON 2 BSD2 (782587..783552) Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification YBR291C 0 783669 784568 ON 2 CTP1 complement(783669..784568) Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family YBR292C 0 784698 785069 OFF 2 YBR292C complement(784698..785069) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene YBR293W 1 787001 788425 ON 2 VBA2 (787001..788425) Permease of basic amino acids in the vacuolar membrane YBR294W 1 789230 791809 ON 2 SUL1 (789230..791809) High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates YBR295W 1 792843 796493 ON 2 PCA1 (792843..796493) Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; S288C and most other lab strains contain a G970R mutation which eliminates normal cadmium transport function YBR296C 0 796792 798516 ON 2 PHO89 complement(796792..798516) Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p YBR296C-A 0 800117 800236 OFF 2 YBR296C-A complement(800117..800236) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR297W 1 800517 801923 ON 2 MAL33 (800517..801923) MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C YBR298C 0 802625 804469 ON 2 MAL31 complement(802625..804469) Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C YBR298C-A 0 805029 805250 OFF 2 YBR298C-A complement(805029..805250) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YBR299W 1 805345 807099 ON 2 MAL32 (805345..807099) Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C YBR300C 0 808594 809091 OFF 2 YBR300C complement(808594..809091) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene YBR301W 1 809051 809413 ON 2 DAN3 (809051..809413) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YBR302C 0 810334 811473 ON 2 COS2 complement(810334..811473) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YCL076W 1 1392 2135 OFF 3 YCL076W (1392..2135) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCL075W 1 2126 2566 OFF 3 YCL075W (2126..2566) Pseudogene: encodes fragment of Ty Pol protein YCL074W 1 2824 3750 OFF 3 YCL074W (2824..3750) Pseudogene: encodes fragment of Ty Pol protein YCL073C 0 6479 8326 ON 3 YCL073C complement(6479..8326) Protein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YKR106W YCL069W 1 9706 11082 ON 3 VBA3 (9706..11082) Permease of basic amino acids in the vacuolar membrane YCL068C 0 11503 12285 ON 3 YCL068C complement(11503..12285) Putative protein of unknown function YCL067C 0 12386 13018 ON 3 HMLALPHA2 complement(12386..13018) Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression YCL066W 1 13282 13809 ON 3 HMLALPHA1 (13282..13809) Silenced copy of ALPHA1 at HML, encoding a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression YCL065W 1 13751 14119 OFF 3 YCL065W (13751..14119) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps HMLALPHA1 YCL064C 0 15798 16880 ON 3 CHA1 complement(15798..16880) Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine YCL063W 1 17290 18561 ON 3 VAC17 (17290..18561) Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p YCL061C 0 18816 22106 ON 3 MRC1 complement(18816..22106) S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint YCL059C 0 22429 23379 ON 3 KRR1 complement(22429..23379) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit YCL058C 0 23523 23981 OFF 3 FYV5 complement(23523..23981) Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in ion homeostasis YCL058W-A 1 23584 23925 ON 3 YCL058W-A (23584..23925) Protein of unknown function; identified by homology to Ashbya gossypii YCL057C-A 0 24032 24325 ON 3 YCL057C-A complement(24032..24325) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YCL057W 1 24768 26906 ON 3 PRD1 (24768..26906) Zinc metalloendopeptidase, found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins YCL056C 0 26925 27359 ON 3 YCL056C complement(26925..27359) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YCL055W 1 27929 28936 ON 3 KAR4 (27929..28936) Transcription factor required for gene regulation in repsonse to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone YCL054W 1 31449 33974 ON 3 SPB1 (31449..33974) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants YCL052C 0 34143 35393 ON 3 PBN1 complement(34143..35393) Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X YCL051W 1 35865 37616 ON 3 LRE1 (35865..37616) Protein involved in control of cell wall structure and stress response; inhibits Cbk1p protein kinase activity; overproduction confers resistance to cell-wall degrading enzymes YCL050C 0 37836 38801 ON 3 APA1 complement(37836..38801) Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa2p YCL049C 0 39786 40724 ON 3 YCL049C complement(39786..40724) Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene; YCL048W-A 1 41488 41727 ON 3 YCL048W-A (41488..41727) Putative protein of unknown function YCL048W 1 42165 43556 ON 3 SPS22 (42165..43556) Protein of unknown function, redundant with Sps2p for the organization of the beta-glucan layer of the spore wall YCL047C 0 43661 44437 ON 3 YCL047C complement(43661..44437) Putative protein of unknown function YCL045C 0 44623 46905 ON 3 YCL045C complement(44623..46905) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; interacts with Gal80p; YCL045C is not an essential gene YCL046W 1 46640 46963 OFF 3 YCL046W (46640..46963) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C YCL044C 0 47111 48364 ON 3 MGR1 complement(47111..48364) Subunit, with Yme1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; required for growth of cells lacking the mitochondrial genome YCL043C 0 48653 50221 ON 3 PDI1 complement(48653..50221) Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds YCL041C 0 50133 50627 OFF 3 YCL041C complement(50133..50627) Hypothetical protein; open reading frame overlaps 5' end of essential PDI1 gene encoding protein disulfide isomerase YCL042W 1 50584 50943 OFF 3 YCL042W (50584..50943) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YCL040W 1 50838 52340 ON 3 GLK1 (50838..52340) Glucokinase, catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources YCL039W 1 52645 54882 ON 3 GID7 (52645..54882) Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions YCL038C 0 54941 56527 ON 3 ATG22 complement(54941..56527) Protein required for the breakdown of autophagic vesicles in the vacuole during autophagy, putative integral membrane protein that localizes to vacuolar membranes and punctate structures attached to the vacuole YCL037C 0 57374 58678 ON 3 SRO9 complement(57374..58678) Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif YCL036W 1 59026 60726 ON 3 GFD2 (59026..60726) Protein of unknown function, identified as a high-copy suppressor of a dbp5 mutation YCL035C 0 60841 61173 ON 3 GRX1 complement(60841..61173) Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair; protects cells from oxidative damage YCL034W 1 61658 62722 ON 3 LSB5 (61658..62722) Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YCL033C 0 62776 63282 ON 3 YCL033C complement(62776..63282) Putative protein-methionine-R-oxide reductase; involved in response to oxidative stress; similar to mouse Sepx1p and fly SelRp; YCL033C is not an essential gene YCL032W 1 63441 64481 ON 3 STE50 (63441..64481) Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction YCL031C 0 64675 65568 ON 3 RRP7 complement(64675..65568) Essential protein involved in rRNA processing and ribosome biogenesis YCL030C 0 65934 68333 ON 3 HIS4 complement(65934..68333) Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis YCL029C 0 68599 69921 ON 3 BIK1 complement(68599..69921) Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 YCL028W 1 70150 71367 ON 3 RNQ1 (70150..71367) [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate YCL027W 1 71803 73341 ON 3 FUS1 (71803..73341) Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate YCL026C-B 0 73405 73986 ON 3 HBN1 complement(73405..73986) Putative protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YCL026C-A 0 74704 75285 ON 3 FRM2 complement(74704..75285) Protein of unknown function, involved in the integration of lipid signaling pathways with cellular homeostasis; similar to bacterial nitroreductases YCL025C 0 76018 77919 ON 3 AGP1 complement(76018..77919) Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) YCL023C 0 78949 79296 OFF 3 YCL023C complement(78949..79296) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 YCL024W 1 79162 82275 ON 3 KCC4 (79162..82275) Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p YCL022C 0 81570 82085 OFF 3 YCL022C complement(81570..82085) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W YCL021W-A 1 83620 83997 ON 3 YCL021W-A (83620..83997) Putative protein of unknown function YCL018W 1 91324 92418 ON 3 LEU2 (91324..92418) Beta-isopropylmalate dehydrogenase (IMDH), catalyzes the third step in the leucine biosynthesis pathway YCL017C 0 92777 94270 ON 3 NFS1 complement(92777..94270) Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; required for the post-transcriptional thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria YCL016C 0 94621 95763 ON 3 DCC1 complement(94621..95763) Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance YCL014W 1 96281 101191 ON 3 BUD3 (96281..101191) Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding YCL012C 0 101317 101788 ON 3 YCL012C complement(101317..101633,101701..101788) Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene YCL011C 0 102075 103358 ON 3 GBP2 complement(102075..103358) Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro YCL010C 0 103571 104350 ON 3 SGF29 complement(103571..104350) SaGa associated Factor 29kDa; Probable 29kKDa Subunit of SAGA histone acetyltransferase complex YCL009C 0 104619 105548 ON 3 ILV6 complement(104619..105548) Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria YCL008C 0 105696 106853 ON 3 STP22 complement(105696..106853) Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype YCL007C 0 106974 107366 OFF 3 YCL007C complement(106974..107366) Dubious ORF unlikely to encode a protein; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A YCL005W-A 1 107023 107417 ON 3 VMA9 (107023..107033,107111..107191,107288..107417) Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis YCL005W 1 108021 108791 ON 3 LDB16 (108021..108791) Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria YCL004W 1 109105 110670 ON 3 PGS1 (109105..110670) Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis YCL002C 0 110843 111674 ON 3 YCL002C complement(110843..111556,111633..111674) Putative protein of unknown function; YCL002C is not an essential gene YCL001W 1 111914 112480 ON 3 RER1 (111914..112480) Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER YCL001W-A 1 113078 113539 OFF 3 YCL001W-A (113078..113539) Putative protein of unknown function; YCL001W-A is not an essential gene YCL001W-B 1 113768 114022 ON 3 YCL001W-B (113768..114022) Putative protein of unknown function; identified by homology YCR001W 1 115683 115997 OFF 3 YCR001W (115683..115997) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene YCR002C 0 117378 118346 ON 3 CDC10 complement(117378..118346) Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM YCR003W 1 118618 119169 ON 3 MRPL32 (118618..119169) Mitochondrial ribosomal protein of the large subunit YCR004C 0 119573 120316 ON 3 YCP4 complement(119573..120316) Protein of unknown function, has sequence and structural similarity to flavodoxins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YCR005C 0 120944 122326 ON 3 CIT2 complement(120944..122326) Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors YCR006C 0 122528 123001 OFF 3 YCR006C complement(122528..123001) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR007C 0 126009 126728 ON 3 YCR007C complement(126009..126728) Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene YCR008W 1 128468 130279 ON 3 SAT4 (128468..130279) Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p YCR009C 0 130743 131540 ON 3 RVS161 complement(130743..131540) Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress YCR010C 0 132271 133122 ON 3 ADY2 complement(132271..133122) Acetate transporter required for normal sporulation YCR011C 0 133722 136871 ON 3 ADP1 complement(133722..136871) Putative ATP-dependent permease of the ABC transporter family of proteins YCR012W 1 137744 138994 ON 3 PGK1 (137744..138994) 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis YCR013C 0 138400 139047 OFF 3 YCR013C complement(138400..139047) ubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in the gcr1 null mutant YCR014C 0 139183 140931 ON 3 POL4 complement(139183..140931) DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta YCR015C 0 141215 142168 ON 3 YCR015C complement(141215..142168) Putative protein of unknown function; YCR015C is not an essential gene YCR016W 1 143632 144504 ON 3 YCR016W (143632..144504) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; YCR016W is not an essential gene YCR017C 0 144771 147632 ON 3 CWH43 complement(144771..147632) Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion YCR018C 0 148236 148901 ON 3 SRD1 complement(148236..148901) Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation YCR018C-A 0 151606 151860 OFF 3 YCR018C-A complement(151606..151860) Putative protein of unknown function; encoded opposite a Ty1 LTR YCR019W 1 152835 153926 ON 3 MAK32 (152835..153926) Protein necessary for structural stability of L-A double-stranded RNA-containing particles YCR020C 0 154010 154657 ON 3 PET18 complement(154010..154657) Protein required for respiratory growth and stability of the mitochondrial genome YCR020C-A 0 154828 155094 ON 3 MAK31 complement(154828..155094) Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family YCR020W-B 1 155318 155554 ON 3 HTL1 (155318..155554) Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance YCR021C 0 156105 157103 ON 3 HSP30 complement(156105..157103) Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase YCR022C 0 157417 157761 OFF 3 YCR022C complement(157417..157761) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene YCR023C 0 158534 160369 ON 3 YCR023C complement(158534..160369) Putative protein of unknown function; YCR023C is not an essential gene YCR024C 0 160740 162218 ON 3 SLM5 complement(160740..162218) Mitochondrial asparaginyl-tRNA synthetase YCR024C-B 0 162595 162861 OFF 3 YCR024C-B complement(162595..162861) Putative protein of unknown function; identified by expression profiling and mass spectrometry YCR024C-A 0 162941 163063 ON 3 PMP1 complement(162941..163063) Small single-membrane span proteolipid that functions as a regulatory subunit of the plasma membrane H(+)-ATPase Pma1p, forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines YCR025C 0 163442 163852 OFF 3 YCR025C complement(163442..163852) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene YCR026C 0 164107 166335 ON 3 NPP1 complement(164107..166335) Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation YCR027C 0 167366 167995 ON 3 RHB1 complement(167366..167995) Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins YCR028C 0 170882 172420 ON 3 FEN2 complement(170882..172420) Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph YCR028C-A 0 172946 173436 ON 3 RIM1 complement(172946..173111,173195..173436) Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication YCR030C 0 173822 176434 ON 3 SYP1 complement(173822..176434) Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation YCR031C 0 177496 178216 ON 3 RPS14A complement(177496..177902,178210..178216) Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins YCR032W 1 179516 186019 ON 3 BPH1 (179516..186019) PProtein homologous to human Chediak-Higashi syndrome and murine Beige proteins, which are implicated in disease syndromes due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting YCR033W 1 186485 190165 ON 3 SNT1 (186485..190165) Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein YCR034W 1 190588 191631 ON 3 FEN1 (190588..191631) Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway YCR035C 0 191830 193014 ON 3 RRP43 complement(191830..193014) Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA YCR036W 1 193293 194294 ON 3 RBK1 (193293..194294) Putative ribokinase YCR037C 0 194410 197181 ON 3 PHO87 complement(194410..197181) Low-affinity inorganic phosphate (Pi) transporter, involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments YCR038C 0 197617 199545 ON 3 BUD5 complement(197617..199545) GTP/GDP exchange factor for Rsr1p (Bud1p) required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types YCR038W-A 1 199289 199414 OFF 3 YCR038W-A (199289..199414) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene BUD5/YCR038C; identified by homology with hemiascomycetous yeast species YCR039C 0 199542 200174 ON 3 MATALPHA2 complement(199542..200174) Homeobox-domain protein that, with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette YCR040W 1 200438 200965 ON 3 MATALPHA1 (200438..200965) Transcriptional co-activator involved in regulation of mating-type-specific gene expression; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette YCR041W 1 200907 201239 OFF 3 YCR041W (200907..201239) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR042C 0 201170 205393 ON 3 TAF2 complement(201170..205393) TFIID subunit (150 kDa), involved in RNA polymerase II transcription initiation YCR043C 0 206257 206640 ON 3 YCR043C complement(206257..206640) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene YCR044C 0 206873 207946 ON 3 PER1 complement(206873..207946) Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 YCR045C 0 208131 209606 ON 3 YCR045C complement(208131..209606) Putative protein of unknown function YCR045W-A 1 208747 209097 OFF 3 YCR045W-A (208747..209097) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with YCR045C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR046C 0 209910 210419 ON 3 IMG1 complement(209910..210419) Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome YCR047C 0 210714 211541 ON 3 BUD23 complement(210714..211541) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YCR047W-A 1 211297 211503 OFF 3 YCR047W-A (211297..211503) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with YCR047C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR049C 0 211867 212313 OFF 3 YCR049C complement(211867..212313) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR048W 1 211925 213757 ON 3 ARE1 (211925..213757) Acyl-CoA:sterol acyltransferase, isozyme of Are2p; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen YCR050C 0 213460 213768 OFF 3 YCR050C complement(213460..213768) Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein YCR051W 1 214067 214735 ON 3 YCR051W (214067..214735) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene YCR052W 1 214990 216441 ON 3 RSC6 (214990..216441) Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p YCR053W 1 216693 218237 ON 3 THR4 (216693..218237) Threonine synthase, conserved protein that catalyzes formation of threonine from 0-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway YCR054C 0 218372 220063 ON 3 CTR86 complement(218372..220063) Essential protein of unknown function; upstream region contains a Gcn4p responsive site suggesting a possible role in amino acid biosynthesis YCR057C 0 220453 223224 ON 3 PWP2 complement(220453..223224) Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis YCR059C 0 223450 224226 ON 3 YIH1 complement(223450..224226) Protein that inhibits activation of Gcn2p, an eIF2 alpha subunit protein kinase, by competing for Gcn1p binding, thus impacting gene expression in response to starvation; has sequence and functional similarity to the mouse IMPACT gene YCR060W 1 224395 224730 ON 3 TAH1 (224395..224730) HSP90 cofactor; interacts with Hsp82p, Pih1p, Rvb1 and Rvb2, contains a single TPR domain with at least two TPR motifs YCR061W 1 225559 227454 ON 3 YCR061W (225559..227454) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YCR064C 0 228091 228501 OFF 3 YCR064C complement(228091..228501) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 YCR063W 1 228314 228787 ON 3 BUD31 (228314..228787) Protein involved in bud-site selection; analysis of integrated high-throughput datasets predicts an involvement in RNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YCR065W 1 229306 231000 ON 3 HCM1 (229306..231000) Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role YCR066W 1 231496 232959 ON 3 RAD18 (231496..232959) Protein involved in postreplication repair; binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; forms heterodimer with Rad6p; contains RING-finger motif YCR067C 0 233121 236318 ON 3 SED4 complement(233121..236318) Integral endoplasmic reticulum membrane protein, functions as a positive regulator of Sar1p probably through inhibition of GTPase activation by Sec23p; binds Sec16p, participates in vesicle formation, similar to Sec12p YCR068W 1 237210 238772 ON 3 ATG15 (237210..238772) Lipase, required for intravacuolar lysis of autophagic bodies; located in the endoplasmic reticulum membrane and targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway YCR069W 1 239051 240007 ON 3 CPR4 (239051..240007) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway YCR071C 0 240099 240539 ON 3 IMG2 complement(240099..240539) Mitochondrial ribosomal protein of the small subunit YCR072C 0 240801 242348 ON 3 RSA4 complement(240801..242348) WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus YCR073C 0 242585 246580 ON 3 SSK22 complement(242585..246580) MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p YCR073W-A 1 246960 247907 ON 3 SOL2 (246960..247907) Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol1p, Sol3p, and Sol4p YCR075C 0 248030 248812 ON 3 ERS1 complement(248030..248812) Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains YCR075W-A 1 248972 249199 ON 3 YCR075W-A (248972..249199) Putative protein of unknown function; identified by homology to Ashbya gossypii YCR076C 0 249290 250042 ON 3 YCR076C complement(249290..250042) Putative protein of unknown function; YCR076C is not an essential gene YCR077C 0 250235 252625 ON 3 PAT1 complement(250235..252625) Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p YCR079W 1 252842 254170 ON 3 PTC6 (252842..254170) Phosphoprotein phosphatase type 2C similar to mammalian PP1Ks; involved in mitophagy; localized to mitochondrial inner membrane space; null mutant is sensitive to rapamycin YCR081W 1 254368 258651 ON 3 SRB8 (254368..258651) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression YCR081C-A 0 258412 258648 OFF 3 YCR081C-A complement(258412..258648) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with SRB8/YCR081W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YCR082W 1 258880 259266 ON 3 AHC2 (258880..259266) Protein of unknown function, putative transcriptional regulator; proposed to be a Ada Histone acetyltransferase complex component; GFP tagged protein is localized to the cytoplasm and nucleus YCR083W 1 259575 259958 ON 3 TRX3 (259575..259958) Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p YCR084C 0 260308 262449 ON 3 TUP1 complement(260308..262449) General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes YCR085W 1 262913 263266 OFF 3 YCR085W (262913..263266) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR086W 1 263389 263961 ON 3 CSM1 (263389..263961) Nucleolar protein that forms a complex with Lrs4p which binds Mam1p at kinetochores during meiosis I to mediate accurate chromosome segregation, may be involved in premeiotic DNA replication; possible role in telomere maintenance YCR087W 1 263973 264488 OFF 3 YCR087W (263973..264488) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene YCR087C-A 0 264003 264464 ON 3 YCR087C-A complement(264003..264464) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene YCR088W 1 265065 266843 ON 3 ABP1 (265065..266843) Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization YCR089W 1 267431 272260 ON 3 FIG2 (267431..272260) Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating YCR090C 0 272312 272860 ON 3 YCR090C complement(272312..272860) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene YCR091W 1 274401 276563 ON 3 KIN82 (274401..276563) Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily YCR092C 0 276761 279904 ON 3 MSH3 complement(276761..279904) Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability YCR093W 1 280114 286440 ON 3 CDC39 (280114..286440) Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor YCR094W 1 286759 287934 ON 3 CDC50 (286759..287934) Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p YCR095C 0 288167 289255 ON 3 YCR095C complement(288167..289255) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YCR095W-A 1 289636 289794 OFF 3 YCR095W-A (289636..289794) Putative protein of unknown function YCR096C 0 293176 293535 OFF 3 HMRA2 complement(293176..293535) Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system YCR097W 1 293832 294318 ON 3 HMRA1 (293832..293936,293991..294236,294289..294318) Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells YCR097W-A 1 294436 294702 OFF 3 YCR097W-A (294436..294702) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by homology to a hemiascomycetous yeast protein YCR098C 0 297046 298602 ON 3 GIT1 complement(297046..298602) Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability YCR099C 0 300829 301296 ON 3 YCR099C complement(300829..301296) Putative protein of unknown function YCR100C 0 301268 302218 ON 3 YCR100C complement(301268..302218) Putative protein of unknown function YCR101C 0 302479 303027 ON 3 YCR101C complement(302479..303027) Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene YCR102C 0 304358 305464 ON 3 YCR102C complement(304358..305464) Putative protein of unknown function; involved in copper metabolism; similar to C.carbonum toxD gene; YCR102C is not an essential gene YCR102W-A 1 306732 306929 OFF 3 YCR102W-A (306732..306929) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YCR104W 1 307798 308172 ON 3 PAU3 (307798..308172) Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YCR105W 1 309067 310152 ON 3 ADH7 (309067..310152) NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance YCR106W 1 310955 313453 ON 3 RDS1 (310955..313453) Zinc cluster transcription factor involved in conferring resistance to cycloheximide YCR107W 1 313887 314978 ON 3 AAD3 (313887..314978) Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YCR108C 0 315994 316185 OFF 3 YCR108C complement(315994..316185) Putative protein of unknown function; identified by fungal homology and RT-PCR YDL248W 1 1802 2953 ON 4 COS7 (1802..2953) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDL247W-A 1 3762 3836 OFF 4 YDL247W-A (3762..3836) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species YDL247W 1 5985 7814 ON 4 MPH2 (5985..7814) Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication YDL246C 0 8683 9756 ON 4 SOR2 complement(8683..9756) Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in fructose or mannose metabolism YDL245C 0 11657 13360 ON 4 HXT15 complement(11657..13360) Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose YDL244W 1 16204 17226 ON 4 THI13 (16204..17226) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YDL243C 0 17577 18566 ON 4 AAD4 complement(17577..18566) Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase, involved in the oxidative stress response YDL242W 1 18959 19312 OFF 4 YDL242W (18959..19312) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDL241W 1 20635 21006 ON 4 YDL241W (20635..21006) Putative protein of unknown function; YDL241W is not an essential gene YDL240C-A 0 22471 22608 OFF 4 YDL240C-A complement(22471..22608) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by sequence comparison with hemiascomycetous yeast species YDL240W 1 22823 25876 ON 4 LRG1 (22823..25876) Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis YDL239C 0 26403 28775 ON 4 ADY3 complement(26403..28775) Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p YDL238C 0 28985 30454 ON 4 GUD1 complement(28985..30454) Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures YDL237W 1 30657 31829 ON 4 YDL237W (30657..31829) Putative protein of unknown function; YDL237W is not an essential gene YDL236W 1 32296 33234 ON 4 PHO13 (32296..33234) Alkaline phosphatase specific for p-nitrophenyl phosphate, involved in dephosphorylation of histone II-A and casein YDL235C 0 33415 33918 ON 4 YPD1 complement(33415..33918) Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus YDL234C 0 34238 36478 ON 4 GYP7 complement(34238..36478) GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking YDL233W 1 36798 38174 ON 4 YDL233W (36798..38174) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL233W is not an essential gene YDL232W 1 38488 38598 ON 4 OST4 (38488..38598) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex YDL231C 0 38868 42245 ON 4 BRE4 complement(38868..42245) Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport YDL230W 1 42701 43708 ON 4 PTP1 (42701..43708) Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria YDL229W 1 44066 45907 ON 4 SSB1 (44066..45907) Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p YDL228C 0 45278 45919 OFF 4 YDL228C complement(45278..45919) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene SSB1 YDL227C 0 46272 48032 ON 4 HO complement(46272..48032) Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p YDL226C 0 51116 52174 ON 4 GCS1 complement(51116..52174) ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p YDL225W 1 52446 54101 ON 4 SHS1 (52446..54101) One of five related septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p, Shs1p) that form a cortical filamentous collar at the mother-bud neck which is necessary for normal morphogenesis and cytokinesis YDL224C 0 54398 56347 ON 4 WHI4 complement(54398..56347) Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division YDL223C 0 57266 60406 ON 4 HBT1 complement(57266..60406) Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis YDL222C 0 60873 61802 ON 4 FMP45 complement(60873..61802) Integral membrane protein localized to mitochondria (untagged protein) and eisosomes, immobile patches at the cortex associated with endocytosis; sporulation and sphingolipid content are altered in mutants; has homologs SUR7 and YNL194C YDL221W 1 62012 62563 OFF 4 YDL221W (62012..62563) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 YDL220C 0 62245 65019 ON 4 CDC13 complement(62245..65019) Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping YDL219W 1 65243 65766 ON 4 DTD1 (65243..65307,65379..65766) D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes YDL218W 1 66494 67447 ON 4 YDL218W (66494..67447) Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions YDL217C 0 67984 68607 ON 4 TIM22 complement(67984..68607) Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane YDL216C 0 68998 70320 ON 4 RRI1 complement(68998..70320) Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling YDL215C 0 70641 73919 ON 4 GDH2 complement(70641..73919) NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels YDL214C 0 74447 76546 ON 4 PRR2 complement(74447..76546) Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor YDL213C 0 77290 77967 ON 4 NOP6 complement(77290..77967) Putative RNA-binding protein implicated in ribosome biogenesis; contains an RNA recognition motif (RRM) and has similarity to hydrophilins; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes YDL212W 1 78427 79059 ON 4 SHR3 (78427..79059) Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface YDL211C 0 79295 80413 ON 4 YDL211C complement(79295..80413) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YDL210W 1 84271 85986 ON 4 UGA4 (84271..85986) Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane YDL209C 0 86208 87227 ON 4 CWC2 complement(86208..87227) Protein involved in pre-mRNA splicing, component of a complex containing Cef1p; interacts with Prp19p; contains an RNA recognition motif; has similarity to S. pombe Cwf2p YDL208W 1 87513 87983 ON 4 NHP2 (87513..87983) Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing YDL207W 1 88249 89865 ON 4 GLE1 (88249..89865) Cytoplasmic nucleoporin required for polyadenylated RNA export but not for protein import; component of Nup82p nuclear pore subcomplex; contains a nuclear export signal YDL206W 1 90177 92465 ON 4 YDL206W (90177..92465) Putative protein of unknown function; YDL206W is not an essential protein YDL205C 0 92763 93746 ON 4 HEM3 complement(92763..93746) Phorphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p YDL204W 1 94606 95787 ON 4 RTN2 (94606..95787) Protein of unknown function; has similarity to mammalian reticulon proteins; member of the RTNLA (reticulon-like A) subfamily YDL203C 0 96083 97954 ON 4 YDL203C complement(96083..97954) Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria YDL202W 1 98476 99225 ON 4 MRPL11 (98476..99225) Mitochondrial ribosomal protein of the large subunit YDL201W 1 99562 100422 ON 4 TRM8 (99562..100422) Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA YDL200C 0 100502 101068 ON 4 MGT1 complement(100502..101068) DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage YDL199C 0 101291 103354 ON 4 YDL199C complement(101291..103354) Putative transporter, member of the sugar porter family YDL198C 0 103650 104552 ON 4 GGC1 complement(103650..104552) Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family YDL197C 0 104918 106495 ON 4 ASF2 complement(104918..106495) Anti-silencing protein that causes derepression of silent loci when overexpressed YDL196W 1 106742 107071 OFF 4 YDL196W (106742..107071) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 YDL195W 1 107209 111030 ON 4 SEC31 (107209..111030) Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation YDL194W 1 111581 114235 ON 4 SNF3 (111581..114235) Plasma membrane glucose sensor that regulates glucose transport; has 12 predicted transmembrane segments; long cytoplasmic C-terminal tail is required for low glucose induction of hexose transporter genes HXT2 and HXT4 YDL193W 1 114673 115800 ON 4 NUS1 (114673..115800) Prenyltransferase, required for cell viability; involved in protein trafficking YDL192W 1 116322 116867 ON 4 ARF1 (116322..116867) ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p YDL191W 1 117665 118518 ON 4 RPL35A (117665..117667,118159..118518) Protein component of the large (60S) ribosomal subunit, identical to Rpl35Bp and has similarity to rat L35 ribosomal protein YDL190C 0 118708 121593 ON 4 UFD2 complement(118708..121593) Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 YDL189W 1 122217 123590 ON 4 RBS1 (122217..123590) Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain YDL188C 0 123866 124999 ON 4 PPH22 complement(123866..124999) Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis YDL187C 0 125510 125839 OFF 4 YDL187C complement(125510..125839) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDL186W 1 125617 126450 ON 4 YDL186W (125617..126450) Putative protein of unknown function; YDL186W is not an essential gene YDL185C-A 0 126609 126836 OFF 4 YDL185C-A complement(126609..126836) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDL185W; identified by homology with hemiascomycetous yeast species YDL185W 1 126788 130003 ON 4 TFP1 (126788..130003) Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease YDL184C 0 130408 130485 ON 4 RPL41A complement(130408..130485) Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Bp and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable YDL183C 0 130872 131834 ON 4 YDL183C complement(130872..131834) Putative protein of unknown function; YBR063C is not an essential gene YDL182W 1 133438 134724 ON 4 LYS20 (133438..134724) Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p YDL181W 1 135180 135437 ON 4 INH1 (135180..135437) Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase, inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has similarity to Stf1p and both Inh1p and Stf1p exhibit the potential to form coiled-coil structures YDL180W 1 135897 137540 ON 4 YDL180W (135897..137540) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YDL179W 1 138292 139206 ON 4 PCL9 (138292..139206) Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p YDL178W 1 139523 141115 ON 4 DLD2 (139523..141115) D-lactate dehydrogenase, located in the mitochondrial matrix YDL177C 0 141210 141722 ON 4 YDL177C complement(141210..141722) Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene YDL176W 1 142098 144224 ON 4 YDL176W (142098..144224) Putative protein of unknown function; YDL176W is not an essential gene YDL175C 0 144485 145519 ON 4 AIR2 complement(144485..145519) RING finger protein that interacts with the arginine methyltransferase Hmt1p; may regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air1p YDL174C 0 145827 147590 ON 4 DLD1 complement(145827..147590) D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane YDL173W 1 148192 149079 ON 4 YDL173W (148192..149079) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDL173W is not an essential gene YDL172C 0 148608 149087 OFF 4 YDL172C complement(148608..149087) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL171C 0 149204 155641 ON 4 GLT1 complement(149204..155641) NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source YDL170W 1 156319 157905 ON 4 UGA3 (156319..157905) Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus YDL169C 0 158065 158736 ON 4 UGX2 complement(158065..158736) Protein of unknown function, transcript accumulates in response to any combination of stress conditions YDL168W 1 159605 160765 ON 4 SFA1 (159605..160765) Bifunctional enzyme containing both alcohol dehydrogenase and glutathione-dependent formaldehyde dehydrogenase activities, functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p YDL167C 0 160996 163155 ON 4 NRP1 complement(160996..163155) Protein of unknown function, rich in asparagine residues YDL166C 0 163450 164043 ON 4 FAP7 complement(163450..164043) Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D YDL165W 1 164291 164866 ON 4 CDC36 (164291..164866) Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor YDL164C 0 164988 167255 ON 4 CDC9 complement(164988..167255) DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination YDL163W 1 166957 167259 OFF 4 YDL163W (166957..167259) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase YDL162C 0 167360 167716 OFF 4 YDL162C complement(167360..167716) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly YDL161W 1 167715 169079 ON 4 ENT1 (167715..169079) Epsin-like protein involved in endocytosis and actin patch assembly and functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus YDL160C-A 0 169367 169609 ON 4 YDL160C-A complement(169367..169609) Putative protein of unknown function YDL160C 0 170411 171931 ON 4 DHH1 complement(170411..171931) Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation YDL159W-A 1 172183 172314 OFF 4 YDL159W-A (172183..172314) Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species YDL159W 1 172482 174029 ON 4 STE7 (172482..174029) Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway YDL159C-B 0 173057 173233 OFF 4 YDL159C-B complement(173057..173233) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDL158C 0 173867 174175 OFF 4 YDL158C complement(173867..174175) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL157C 0 174233 174589 ON 4 YDL157C complement(174233..174589) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDL156W 1 174919 176487 ON 4 YDL156W (174919..176487) Putative protein of unknown function; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YDL155W 1 176774 178057 ON 4 CLB3 (176774..178057) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation YDL154W 1 178335 181040 ON 4 MSH5 (178335..181040) Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans YDL153C 0 181187 183019 ON 4 SAS10 complement(181187..183019) Component of the small (ribosomal) subunit (SSU) processosome required for pre-18S rRNa processing; essential nucleolar protein that, when overproduced, disrupts silencing YDL152W 1 182823 183188 OFF 4 YDL152W (182823..183188) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome YDL151C 0 183319 183900 OFF 4 BUD30 complement(183319..183900) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay YDL150W 1 183344 184612 ON 4 RPC53 (183344..184612) RNA polymerase III subunit C53 YDL149W 1 184926 187919 ON 4 ATG9 (184926..187919) Transmembrane protein involved in formation of Cvt and autophagic vesicles; cycles between the pre-autophagosomal structure and other cytosolic punctate structures, not found in autophagosomes YDL148C 0 188155 190587 ON 4 NOP14 complement(188155..190587) Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA YDL147W 1 190925 192262 ON 4 RPN5 (190925..192262) Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p YDL146W 1 192751 194226 ON 4 LDB17 (192751..194226) Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface YDL145C 0 194572 198177 ON 4 COP1 complement(194572..198177) Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway YDL144C 0 198663 199733 ON 4 YDL144C complement(198663..199733) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene. YDL143W 1 199997 201583 ON 4 CCT4 (199997..201583) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YDL142C 0 201720 202571 ON 4 CRD1 complement(201720..202571) Cardiolipin synthase; produces cardiolipin, which is an important constituent of mitochondrial membranes; required for normal mitochondrial membrane potential and function YDL141W 1 203040 205112 ON 4 BPL1 (203040..205112) Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation YDL140C 0 205361 210562 ON 4 RPO21 complement(205361..210562) RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime YDL139C 0 211376 212047 ON 4 SCM3 complement(211376..212047) Protein of unknown function; contains nuclear export signal (NES); associates with CEN DNA; required for G2/M progression and localization of Cse4p; isolated as a high-copy suppressor of mutations in the histone fold domain (HFD) of CSE4 YDL138W 1 213352 215643 ON 4 RGT2 (213352..215643) Plasma membrane glucose receptor, highly similar to Snf3p; both Rgt2p and Snf3p serve as transmembrane glucose sensors generating an intracellular signal that induces expression of glucose transporter (HXT) genes YDL137W 1 216529 217074 ON 4 ARF2 (216529..217074) ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf1p YDL136W 1 217600 218367 ON 4 RPL35B (217600..217602,218008..218367) Protein component of the large (60S) ribosomal subunit, identical to Rpl35Ap and has similarity to rat L35 ribosomal protein YDL135C 0 218680 219288 ON 4 RDI1 complement(218680..219288) Rho GDP dissociation inhibitor involved in the localization and regulation of Cdc42p YDL134C 0 219662 220771 ON 4 PPH21 complement(219662..220771) Catalytic subunit of protein phosphatase 2A, functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis YDL133C-A 0 221724 221801 ON 4 RPL41B complement(221724..221801) Ribosomal protein L47 of the large (60S) ribosomal subunit, identical to Rpl41Ap and has similarity to rat L41 ribosomal protein; comprised of only 25 amino acids; rpl41a rpl41b double null mutant is viable YDL133W 1 222427 223740 ON 4 YDL133W (222427..223740) Putative protein of unknown function YDL132W 1 224304 226751 ON 4 CDC53 (224304..226751) Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation YDL131W 1 227393 228715 ON 4 LYS21 (227393..228715) Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p YDL130W-A 1 229171 229431 ON 4 STF1 (229171..229431) Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein YDL130W 1 229906 230527 ON 4 RPP1B (229906..230019,230321..230527) Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component YDL129W 1 231024 231899 ON 4 YDL129W (231024..231899) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene YDL128W 1 232652 233887 ON 4 VCX1 (232652..233887) Vacuolar H+/Ca2+ exchanger involved in control of cytosolic Ca2+ concentration; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter YDL127W 1 234927 235853 ON 4 PCL2 (234927..235853) G1 cyclin, associates with Pho85p cyclin-dependent kinase (Cdk) to contribute to entry into the mitotic cell cycle, essential for cell morphogenesis; localizes to sites of polarized cell growth YDL126C 0 236157 238664 ON 4 CDC48 complement(236157..238664) ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome YDL125C 0 239019 239606 ON 4 HNT1 complement(239019..239398,239510..239606) Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint YDL124W 1 240259 241197 ON 4 YDL124W (240259..241197) NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family YDL123W 1 241418 241840 ON 4 SNA4 (241418..241840) Protein of unknown function, localized to the vacuolar outer membrane YDL122W 1 242552 244981 ON 4 UBP1 (242552..244981) Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains YDL121C 0 245133 245582 ON 4 YDL121C complement(245133..245582) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic retiuculum; YDL121C is not an essential protein YDL120W 1 245923 246447 ON 4 YFH1 (245923..246447) Frataxin, regulates mitochondrial iron accumulation; interacts with Isu1p which promotes Fe-S cluster assembly; interacts with electron transport chain components and may influence respiration; human homolog involved in Friedrich's ataxia YDL119C 0 246689 247612 ON 4 YDL119C complement(246689..247612) Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria YDL118W 1 247302 247682 OFF 4 YDL118W (247302..247682) Non-essential protein of unconfirmed function; mutants are defective in telomere maintenance, and are synthetically sick or lethal with alpha-synuclein YDL117W 1 248581 251238 ON 4 CYK3 (248581..251238) SH3-domain protein located in the mother-bud neck and the cytokinetic actin ring; mutant phenotype and genetic interactions suggest a role in cytokinesis YDL116W 1 251566 253746 ON 4 NUP84 (251566..253746) Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis YDL115C 0 253995 255126 ON 4 IWR1 complement(253995..254974,255045..255126) Protein of unknown function, deletion causes hypersensitivity to the K1 killer toxin YDL114W-A 1 254935 255048 OFF 4 YDL114W-A (254935..255048) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified based on homology to hemiascomycetous yeasts YDL114W 1 255604 256530 ON 4 YDL114W (255604..256530) Putative protein of unknown function with similarity to acyl-carrier-protein reductases; YDL114W is not an essential gene YDL113C 0 256633 258555 ON 4 ATG20 complement(256633..258555) Protein required for transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway; binds phosphatidylinositol-3-phosphate, involved in localization of membranes to the preautophagosome, potential Cdc28p substrate YDL112W 1 258915 263225 ON 4 TRM3 (258915..263225) 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs YDL111C 0 263313 264110 ON 4 RRP42 complement(263313..264110) Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp4p, Rrp41p, Rrp43p and Dis3p YDL110C 0 264512 264964 ON 4 TMA17 complement(264512..264964) Protein of unknown function that associates with ribosomes YDL109C 0 265258 267201 ON 4 YDL109C complement(265258..267201) Putative lipase; involved in lipid metabolism; YDL109C is not an essential gene YDL108W 1 267698 268699 ON 4 KIN28 (267698..267725,267807..268699) Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters YDL107W 1 268921 269976 ON 4 MSS2 (268921..269976) Peripherally bound inner membrane protein of the mitochondrial matrix, binds to the Cox2p C-terminal tail to facilitate its translocation through the inner membrane YDL106C 0 270222 271901 ON 4 PHO2 complement(270222..271901) Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p YDL105W 1 272389 273597 ON 4 NSE4 (272389..273597) Nuclear protein that plays a role in the function of the Smc5p-Rhc18p complex YDL104C 0 273653 274876 ON 4 QRI7 complement(273653..274876) Putative metalloprotease, similar to O-sialoglycoprotein metallopeptidase from P. haemolytica; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDL103C 0 275148 276581 ON 4 QRI1 complement(275148..276581) UDP-N-acetylglucosamine pyrophosphorylase, catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis YDL102W 1 276872 280165 ON 4 POL3 (276872..280165) Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) YDL101C 0 280307 281848 ON 4 DUN1 complement(280307..281848) Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair YDL100C 0 282112 283176 ON 4 GET3 complement(282112..283176) ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress YDL099W 1 283419 284444 ON 4 BUG1 (283419..284444) Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes YDL098C 0 284581 285165 ON 4 SNU23 complement(284581..285165) Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome YDL097C 0 285391 286695 ON 4 RPN6 complement(285391..286695) Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion YDL096C 0 286998 287324 OFF 4 OPI6 complement(286998..287324) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential YDL095W 1 287059 289512 ON 4 PMT1 (287059..289512) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt2p, can instead interact with Pmt3p in some conditions; target for new antifungals YDL094C 0 289572 290081 OFF 4 YDL094C complement(289572..290081) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential YDL093W 1 289909 292140 ON 4 PMT5 (289909..292140) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals YDL092W 1 292781 293221 ON 4 SRP14 (292781..293221) Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 YDL091C 0 293392 294759 ON 4 UBX3 complement(293392..294759) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDL090C 0 295034 296329 ON 4 RAM1 complement(295034..296329) Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit YDL089W 1 296820 298274 ON 4 YDL089W (296820..298274) Protein of unknown function; interacts with meiotic division protein Csm1p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery, potential Cdc28p substrate YDL088C 0 298417 300003 ON 4 ASM4 complement(298417..300003) Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p YDL087C 0 300214 300999 ON 4 LUC7 complement(300214..300999) Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site YDL086W 1 301413 302234 ON 4 YDL086W (301413..302234) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene YDL086C-A 0 301655 302089 OFF 4 YDL086C-A complement(301655..302089) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps uncharacterized ORF YDL086W; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YDL085C-A 0 302464 302670 ON 4 YDL085C-A complement(302464..302670) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YDL085W 1 303211 304848 ON 4 NDE2 (303211..304848) Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain YDL084W 1 305237 306577 ON 4 SUB2 (305237..306577) Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56 YDL083C 0 306926 307789 ON 4 RPS16B complement(306926..307333,307766..307789) Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins YDL082W 1 308424 309388 ON 4 RPL13A (308424..308427,308793..309388) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential for viability; has similarity to rat L13 ribosomal protein YDL081C 0 309802 310122 ON 4 RPP1A complement(309802..310122) Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component YDL080C 0 310642 312471 ON 4 THI3 complement(310642..312471) Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis YDL079C 0 312951 314748 ON 4 MRK1 complement(312951..314044,314337..314748) Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation YDL078C 0 315357 316388 ON 4 MDH3 complement(315357..316388) Cytoplasmic malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle YDL077C 0 316971 320120 ON 4 VAM6 complement(316971..320120) Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p YDL076C 0 320668 321552 ON 4 RXT3 complement(320668..321552) Subunit of the RPD3L complex; involved in histone deacetylation YDL075W 1 322226 322988 ON 4 RPL31A (322226..322282,322704..322988) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p YDL074C 0 324047 326149 ON 4 BRE1 complement(324047..326149) E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain YDL073W 1 326613 329567 ON 4 YDL073W (326613..329567) Putative protein of unknown function; YDL073W is not an essential gene YDL072C 0 329836 330447 ON 4 YET3 complement(329836..330447) Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein YDL071C 0 330667 331041 OFF 4 YDL071C complement(330667..331041) Hypothetical protein YDL070W 1 331025 332941 ON 4 BDF2 (331025..332941) Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p YDL069C 0 333121 333810 ON 4 CBS1 complement(333121..333810) Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader YDL068W 1 333500 333829 OFF 4 YDL068W (333500..333829) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL067C 0 334217 334396 ON 4 COX9 complement(334217..334396) Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain YDL066W 1 334835 336121 ON 4 IDP1 (334835..336121) Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes YDL065C 0 336249 337277 ON 4 PEX19 complement(336249..337277) Chaperone and import receptor for newly-synthesized class I peroxisomal membrane proteins (PMPs), binds PMPs in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane YDL064W 1 337487 338070 ON 4 UBC9 (337487..337524,337635..338070) SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) YDL063C 0 338272 340134 ON 4 YDL063C complement(338272..340134) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL063C is not an essential gene YDL062W 1 339857 340282 OFF 4 YDL062W (339857..340282) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential YDL061C 0 340628 340798 ON 4 RPS29B complement(340628..340798) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similarity to rat S29 and E. coli S14 ribosomal proteins YDL060W 1 341619 343985 ON 4 TSR1 (341619..343985) Protein required for processing of 20S pre-rRNA in the cytoplasm, associates with pre-40S ribosomal particles YDL059C 0 344237 344953 ON 4 RAD59 complement(344237..344953) Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p YDL058W 1 345665 351037 ON 4 USO1 (345665..351037) Essential protein involved in intracellular protein transport, coiled-coil protein necessary for transport from ER to Golgi; required for assembly of the ER-to-Golgi SNARE complex YDL057W 1 351434 352420 ON 4 YDL057W (351434..352420) Putative protein of unknown function; YDL057W is not an essential gene YDL056W 1 352877 355378 ON 4 MBP1 (352877..355378) Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes YDL055C 0 355674 356759 ON 4 PSA1 complement(355674..356759) GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure YDL054C 0 359825 361285 ON 4 MCH1 complement(359825..361285) Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport YDL053C 0 361699 362256 ON 4 PBP4 complement(361699..362256) Pbp1p binding protein, interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay YDL052C 0 362672 363583 ON 4 SLC1 complement(362672..363583) 1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; located in lipid particles and endoplasmic reticulum YDL051W 1 363952 364779 ON 4 LHP1 (363952..364779) RNA binding protein required for maturation of tRNA and snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen YDL050C 0 364446 364817 OFF 4 YDL050C complement(364446..364817) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL049C 0 365068 365874 ON 4 KNH1 complement(365068..365874) Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant YDL048C 0 366739 368211 ON 4 STP4 complement(366739..368211) Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p YDL047W 1 369769 370704 ON 4 SIT4 (369769..370704) Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization YDL046W 1 371237 371758 ON 4 NPC2 (371237..371758) Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes YDL045W-A 1 372245 372532 ON 4 MRP10 (372245..372532) Mitochondrial ribosomal protein of the small subunit YDL045C 0 372685 373605 ON 4 FAD1 complement(372685..373605) Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin YDL044C 0 373964 375286 ON 4 MTF2 complement(373964..375286) Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription YDL043C 0 375677 376477 ON 4 PRP11 complement(375677..376477) Subunit of the SF3a splicing factor complex, required for spliceosome assembly YDL042C 0 376754 378442 ON 4 SIR2 complement(376754..378442) Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication YDL041W 1 378099 378452 OFF 4 YDL041W (378099..378452) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C YDL040C 0 378871 381435 ON 4 NAT1 complement(378871..381435) Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing YDL039C 0 381983 382330 OFF 4 PRM7 complement(381983..382330) Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements YDL038C 0 382327 384078 OFF 4 YDL038C complement(382327..384078) Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YDL037C 0 384598 385584 ON 4 BSC1 complement(384598..385584) Protein of unconfirmed function, similar to cell surface flocculin Muc1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression YDL036C 0 387510 388898 ON 4 PUS9 complement(387510..388898) Mitochondrial tRNA pseudouridine synthase involved in pseudouridylation of mitochondrial tRNAs at position 32 YDL035C 0 389169 392054 ON 4 GPR1 complement(389169..392054) Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis YDL034W 1 391780 392124 OFF 4 YDL034W (391780..392124) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene YDL033C 0 392656 393909 ON 4 SLM3 complement(392656..393909) tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) YDL032W 1 393687 393998 OFF 4 YDL032W (393687..393998) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene YDL031W 1 394214 397201 ON 4 DBP10 (394214..397201) Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis YDL030W 1 397534 399126 ON 4 PRP9 (397534..399126) Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex YDL029W 1 399337 400635 ON 4 ARP2 (399337..399358,399482..400635) Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity YDL028C 0 400994 403288 ON 4 MPS1 complement(400994..403288) Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p YDL027C 0 403692 404954 ON 4 YDL027C complement(403692..404954) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene YDL026W 1 404746 405057 OFF 4 YDL026W (404746..405057) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDL025C 0 405341 407203 ON 4 YDL025C complement(405341..407203) Putative protein kinase, potentially phosphorylated by Cdc28p; YDL025C is not an essential gene YDL025W-A 1 405407 405511 OFF 4 YDL025W-A (405407..405511) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the ORF YDL025C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YDL024C 0 408448 409854 ON 4 DIA3 complement(408448..409854) Protein of unknown function, involved in invasive and pseudohyphal growth YDL022C-A 0 409805 410053 OFF 4 YDL022C-A complement(409805..410053) Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene DIA3; identified by fungal homology and RT-PCR YDL023C 0 411758 412078 OFF 4 YDL023C complement(411758..412078) Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance YDL022W 1 411822 412997 ON 4 GPD1 (411822..412997) NAD-dependent glycerol-3-phosphate dehydrogenase, key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p YDL021W 1 413950 414885 ON 4 GPM2 (413950..414885) Homolog of Gpm1p phosphoglycerate mutase which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event YDL020C 0 415110 416705 ON 4 RPN4 complement(415110..416705) Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses YDL019C 0 417660 421511 ON 4 OSH2 complement(417660..421511) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YDL018C 0 422830 423507 ON 4 ERP3 complement(422830..423507) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport YDL017W 1 424206 425729 ON 4 CDC7 (424206..425729) DDK (Dbf4-dependent kinase) catalytic subunit required for firing origins and replication fork progression in mitosis through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression YDL016C 0 425567 425869 OFF 4 YDL016C complement(425567..425869) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication YDL015C 0 425999 426931 ON 4 TSC13 complement(425999..426931) Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4p YDL014W 1 427361 428344 ON 4 NOP1 (427361..428344) Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin YDL013W 1 429064 430923 ON 4 SLX5 (429064..430923) Protein containing a RING finger domain that interacts with Slx8p; mutant phenotypes and genetic interactions suggest a role in sumoylation and in genome stability YDL012C 0 431105 431514 ON 4 YDL012C complement(431105..431383,431470..431514) Plasma membrane protein of unknown function; YDL012C is not an essential gene YDL011C 0 432305 432628 OFF 4 YDL011C complement(432305..432628) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W YDL010W 1 432327 433022 ON 4 YDL010W (432327..433022) Putative thiol-disulfide oxidoreductase; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL010W is not an essential gene YDL009C 0 432922 433245 OFF 4 YDL009C complement(432922..433245) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YDL010W; YDL009C is not an essential gene YDL008W 1 433494 433991 ON 4 APC11 (433494..433991) Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YDL007C-A 0 436564 436821 ON 4 YDL007C-A complement(436564..436821) Putative protein of unknown function YDL007W 1 438044 439357 ON 4 RPT2 (438044..439357) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle YDL006W 1 439906 440751 ON 4 PTC1 (439906..440751) Type 2C protein phosphatase (PP2C); inactivates the osmosensing MAPK cascade by dephosphorylating Hog1p; mutation delays mitochondrial inheritance; deletion reveals defects in precursor tRNA splicing, sporulation and cell separation YDL005C 0 441011 442306 ON 4 MED2 complement(441011..442306) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YDL004W 1 443026 443508 ON 4 ATP16 (443026..443508) Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YDL003W 1 444680 446380 ON 4 MCD1 (444680..446380) Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase YDL002C 0 446964 447575 ON 4 NHP10 complement(446964..447575) Protein related to mammalian high mobility group proteins; likely component of the INO80 complex, which is an ATP-dependent chromatin-remodeling complex YDL001W 1 447981 449273 ON 4 RMD1 (447981..449273) Cytoplasmic protein required for sporulation YDR001C 0 450217 452472 ON 4 NTH1 complement(450217..452472) Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p YDR002W 1 453042 453647 ON 4 YRB1 (453042..453647) Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 YDR003W 1 454119 454751 ON 4 RCR2 (454119..454751) Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; similar to Rcr1p YDR003W-A 1 454779 454901 OFF 4 YDR003W-A (454779..454901) Putative protein of unknown function; identified by expression profiling and mass spectrometry YDR004W 1 455198 456580 ON 4 RAD57 (455198..456580) Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p YDR005C 0 456833 458100 ON 4 MAF1 complement(456833..458014,458095..458100) Negative regulator of RNA polymerase III; component of several signaling pathways that repress polymerase III transcription in response to changes in cellular environment; targets the initiation factor TFIIIB YDR006C 0 458539 461244 ON 4 SOK1 complement(458539..461244) Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 YDR007W 1 461839 462513 ON 4 TRP1 (461839..462513) Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) YDR008C 0 462249 462599 OFF 4 YDR008C complement(462249..462599) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDR009W 1 463431 464993 ON 4 GAL3 (463431..464993) Transcriptional regulator involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity YDR010C 0 465048 465380 OFF 4 YDR010C complement(465048..465380) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YDR011W 1 465916 470421 ON 4 SNQ2 (465916..470421) Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species YDR012W 1 471850 472938 ON 4 RPL4B (471850..472938) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins YDR013W 1 473154 473780 ON 4 PSF1 (473154..473780) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YDR014W 1 474043 475986 ON 4 RAD61 (474043..475986) Protein of unknown function; mutation confers radiation sensitivity YDR014W-A 1 477794 478282 ON 4 HED1 (477794..478282) Meiosis-specific protein that down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; early meiotic gene, transcribed specifically during meiotic prophase YDR015C 0 477957 478196 OFF 4 YDR015C complement(477957..478196) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A YDR016C 0 478471 478755 ON 4 DAD1 complement(478471..478755) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YDR017C 0 479112 482264 ON 4 KCS1 complement(479112..482264) Inositol hexakisphosphate (IP6) kinase, also has inositol heptakisphosphate (IP7) kinase activity, required for proper vacuole morphology and involved in salt stress response; contains two leucine heptad repeats YDR018C 0 482667 483857 ON 4 YDR018C complement(482667..483857) Probable membrane protein with three predicted transmembrane domains; homologous to Ybr042cp, similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; null exhibits no apparent phenotype YDR019C 0 484160 485362 ON 4 GCV1 complement(484160..485362) T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm YDR020C 0 485743 486441 ON 4 YDR020C complement(485743..486441) Predicted protein shares weak similarity with uridine kinases and with phosphoribokinases; null exhibits no apparent phenotype YDR021W 1 486801 488000 ON 4 FAL1 (486801..488000) Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases YDR022C 0 488069 488659 ON 4 CIS1 complement(488069..488659) Protein required for autophagosome formation in concert with Atg17p; may be involved in microtubule organization; high-copy suppressor of CIK1 deletion YDR023W 1 489505 490893 ON 4 SES1 (489505..490893) Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p YDR024W 1 491014 491499 OFF 4 FYV1 (491014..491499) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin YDR025W 1 491512 492321 ON 4 RPS11A (491512..491556,491896..492321) Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins YDR026C 0 492553 494265 ON 4 YDR026C complement(492553..494265) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein that may bind to the Ter region of rDNA; interacts physically with Fob1p YDR027C 0 494646 497315 ON 4 VPS54 complement(494646..497315) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; potentially phosphorylated by Cdc28p YDR028C 0 497832 500876 ON 4 REG1 complement(497832..500876) Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes YDR029W 1 501097 501411 OFF 4 YDR029W (501097..501411) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data YDR030C 0 501750 503270 ON 4 RAD28 complement(501750..503270) Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSA protein YDR031W 1 503495 503860 ON 4 MIC14 (503495..503860) Mitochondrial intermembrane space cysteine motif protein of 14 kDa YDR032C 0 504096 504692 ON 4 PST2 complement(504096..504692) Protein with similarity to members of a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR033W 1 508144 509106 ON 4 MRH1 (508144..509106) Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; has similarity to Hsp30p and Yro2p, which are induced during heat shock YDR034C 0 509734 512106 ON 4 LYS14 complement(509734..512106) Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer YDR034C-A 0 520513 520689 OFF 4 YDR034C-A complement(520513..520689) Putative protein of unknown function; contained within the solo Ty1 LTR element YDRWdelta7 YDR034W-B 1 521311 521466 ON 4 YDR034W-B (521311..521466) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YDR035W 1 521813 522925 ON 4 ARO3 (521813..522925) 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan YDR036C 0 523208 524710 ON 4 EHD3 complement(523208..524710) Protein of unconfirmed function, plays an indirect role in endocytic membrane trafficking, member of a family of enoyl-CoA hydratase/isomerases; the non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR037W 1 525437 527212 ON 4 KRS1 (525437..527212) Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis YDR038C 0 527419 530694 ON 4 ENA5 complement(527419..530694) Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family YDR039C 0 531304 534579 ON 4 ENA2 complement(531304..534579) P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux YDR040C 0 535189 538464 ON 4 ENA1 complement(535189..538464) P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance YDR041W 1 539800 540411 ON 4 RSM10 (539800..540411) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins YDR042C 0 540598 541200 ON 4 YDR042C complement(540598..541200) Putative protein of unknown function; expression is increased in ssu72-ts69 mutant YDR043C 0 542672 543367 ON 4 NRG1 complement(542672..543367) Transcriptional repressor that recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response YDR044W 1 546640 547626 ON 4 HEM13 (546640..547626) Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; localizes to the mitochondrial inner membrane; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) YDR045C 0 547976 548308 ON 4 RPC11 complement(547976..548308) RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS YDR046C 0 548760 550574 ON 4 BAP3 complement(548760..550574) Amino acid permease involved in the uptake of cysteine, leucine, isoleucine and valine YDR047W 1 551858 552946 ON 4 HEM12 (551858..552946) Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; activity inhibited by Cu2+, Zn2+, Fe2+, Fe3+ and sulfhydryl-specific reagents YDR048C 0 553082 553396 OFF 4 YDR048C complement(553082..553396) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR049W 1 553252 555150 ON 4 YDR049W (553252..555150) Zinc finger protein; putative transcription factor that may interact with proteins involved in histone acetylation or deacetylation; may be involved in altering acetylation on histone lysines YDR050C 0 555724 556470 ON 4 TPI1 complement(555724..556470) Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region YDR051C 0 557054 558058 ON 4 YDR051C complement(557054..558058) Protein of unknown function; interacts with Hsp82p in two-hybrid assay; deletion confers sensitivity to Nickel YDR052C 0 558509 560623 ON 4 DBF4 complement(558509..560623) Regulatory subunit of Cdc7p-Dbf4p kinase complex, required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated YDR053W 1 560247 560642 OFF 4 YDR053W (560247..560642) Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex YDR054C 0 561438 562325 ON 4 CDC34 complement(561438..562325) Ubiquitin-conjugating enzyme (E2) and catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation YDR055W 1 563525 564859 ON 4 PST1 (563525..564859) Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 YDR056C 0 565021 565638 ON 4 YDR056C complement(565021..565638) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic retiuculum; YDR056C is not an essential protein YDR057W 1 565925 567553 ON 4 YOS9 (565925..567553) ER quality-control lectin; integral subunit of the HRD ligase; serves as a receptor for misfolded N-glycosylated proteins, participates in their targeting to ERAD; member of the OS-9 protein family YDR058C 0 567721 568701 ON 4 TGL2 complement(567721..568701) Protein with lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli; role in yeast lipid degradation is unclear YDR059C 0 569232 569768 ON 4 UBC5 complement(569232..569631,569722..569768) Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins, central component of the cellular stress response; expression is heat inducible YDR060W 1 570646 573723 ON 4 MAK21 (570646..573723) Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein YDR061W 1 574161 575780 ON 4 YDR061W (574161..575780) Mitochondrial ATP-binding cassette (ABC) transporter; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance YDR062W 1 576471 578156 ON 4 LCB2 (576471..578156) Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine YDR063W 1 578661 579110 ON 4 YDR063W (578661..579110) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDR063W is not an essential gene YDR064W 1 579456 580450 ON 4 RPS13 (579456..579476,580016..580450) Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins YDR065W 1 580685 581782 ON 4 YDR065W (580685..581782) Protein of unknown function, required for vacuolar acidification; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR066C 0 581906 582496 ON 4 YDR066C complement(581906..582496) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene YDR067C 0 582789 583463 ON 4 YDR067C complement(582789..583463) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YDR068W 1 583711 584643 ON 4 DOS2 (583711..584643) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR069C 0 584937 587717 ON 4 DOA4 complement(584937..587717) Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole YDR070C 0 588096 588377 ON 4 YDR070C complement(588096..588377) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR071C 0 588825 589400 ON 4 PAA1 complement(588825..589400) Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication YDR072C 0 589759 591342 ON 4 IPT1 complement(589759..591342) Inositolphosphotransferase 1, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), which is the most abundant sphingolipid in cells, mutation confers resistance to the antifungals syringomycin E and DmAMP1 in some growth media YDR073W 1 592436 592945 ON 4 SNF11 (592436..592945) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p YDR074W 1 593890 596580 ON 4 TPS2 (593890..596580) Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway YDR075W 1 597153 598079 ON 4 PPH3 (597153..598079) Catalytic subunit of an evolutionarily conserved protein phosphatase complex containing Psy2p and the regulatory subunit Psy4p; required for cisplatin resistance; involved in activation of Gln3p YDR076W 1 598465 599685 ON 4 RAD55 (598465..599685) Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p YDR077W 1 600790 601806 ON 4 SED1 (600790..601806) Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites YDR078C 0 602195 602866 ON 4 SHU2 complement(602195..602866) Protein of unassigned function involved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation; associates with Shu1p, Psy3p, and Csm2p YDR079W 1 603061 603396 ON 4 PET100 (603061..603396) Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme YDR079C-A 0 603590 603808 ON 4 TFB5 complement(603590..603808) Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation; homolog of the Chlamydomonas reinhardtii REX1-S protein which is involved in DNA repair YDR080W 1 604005 606983 ON 4 VPS41 (604005..606983) Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport YDR081C 0 607301 610078 ON 4 PDC2 complement(607301..610078) Transcription factor required for the synthesis of the glycolytic enzyme pyruvate decarboxylase, required for high level expression of both the THI and the PDC genes YDR082W 1 610438 611922 ON 4 STN1 (610438..611922) Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping YDR083W 1 612070 613248 ON 4 RRP8 (612070..613248) Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation YDR084C 0 613402 614001 ON 4 TVP23 complement(613402..614001) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR085C 0 614285 616147 ON 4 AFR1 complement(614285..616147) Alpha-factor pheromone receptor regulator, negatively regulates pheromone receptor signaling; required for normal mating projection (shmoo) formation; required for Spa2p to recruit Mpk1p to shmoo tip during mating; interacts with Cdc12p YDR086C 0 616925 617167 ON 4 SSS1 complement(616925..617167) Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p YDR087C 0 617467 618303 ON 4 RRP1 complement(617467..618303) Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles YDR088C 0 618494 619642 ON 4 SLU7 complement(618494..619642) RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain YDR089W 1 622109 624718 ON 4 YDR089W (622109..624718) Protein of unknown function; deletion confers resistance to Nickel YDR090C 0 625063 625995 ON 4 YDR090C complement(625063..625995) Putative protein of unknown function YDR091C 0 626705 628531 ON 4 RLI1 complement(626705..628531) Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPase YDR092W 1 629873 630602 ON 4 UBC13 (629873..629902,630171..630602) Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus YDR093W 1 631279 636117 ON 4 DNF2 (631279..636117) Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase YDR094W 1 635837 636172 OFF 4 YDR094W (635837..636172) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 YDR095C 0 636114 636524 OFF 4 YDR095C complement(636114..636524) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR096W 1 637136 639820 ON 4 GIS1 (637136..639820) JmjC domain-containing histone demethylase; transcription factor involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1 YDR097C 0 640106 643834 ON 4 MSH6 complement(640106..643834) Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p YDR098C 0 644175 645032 ON 4 GRX3 complement(644175..645032) Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage YDR099W 1 653604 654425 ON 4 BMH2 (653604..654425) 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling YDR100W 1 655010 655441 ON 4 TVP15 (655010..655441) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p YDR101C 0 655683 657464 ON 4 ARX1 complement(655683..657464) Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex YDR102C 0 657645 657977 OFF 4 YDR102C complement(657645..657977) Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index YDR103W 1 658347 661100 ON 4 STE5 (658347..661100) Pheromone-responsive scaffold protein; shuttles from the nucleus to the plasma membrane and assembles kinases Ste11p, Ste7p, and Fus3p into a specific signaling complex; active oligomeric form interacts with Ste4p-Ste18p complex YDR104C 0 661170 664907 ON 4 SPO71 complement(661170..664907) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis YDR105C 0 665346 666767 ON 4 TMS1 complement(665346..666767) Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance YDR106W 1 667001 667855 ON 4 ARP10 (667001..667855) Component of the dynactin complex, localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex YDR107C 0 669013 671031 ON 4 YDR107C complement(669013..671031) Multispanning membrane protein of unknown function YDR108W 1 671266 673362 ON 4 GSG1 (671266..673362) Subunit of TRAPP (transport protein particle), a multi-subunit complex involved in targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment; protein has late meiotic role, following DNA replication YDR109C 0 673517 675664 ON 4 YDR109C complement(673517..675664) Putative kinase YDR110W 1 676099 677799 ON 4 FOB1 (676099..677799) Nucleolar protein required for DNA replication fork blocking and recombinational hotspot activities; binds to the replication fork barrier site in the rDNA region; related to retroviral integrases YDR111C 0 678238 679761 ON 4 YDR111C complement(678238..679761) Putative alanine transaminase (glutamic pyruvic transaminase) YDR112W 1 679541 679849 OFF 4 YDR112W (679541..679849) Hypothetical protein, null mutant displays increased levels of spontaneous Rad52 foci YDR113C 0 680493 681614 ON 4 PDS1 complement(680493..681614) Securin that inhibits anaphase by binding separin Esp1p, also blocks cyclin destruction and mitotic exit, essential for cell cycle arrest in mitosis in the presence of DNA damage or aberrant mitotic spindles; also present in meiotic nuclei YDR114C 0 681921 682223 OFF 4 YDR114C complement(681921..682223) Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium YDR115W 1 682172 682489 ON 4 YDR115W (682172..682489) Putative mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins YDR116C 0 682721 683578 ON 4 MRPL1 complement(682721..683578) Mitochondrial ribosomal protein of the large subunit YDR117C 0 683943 685640 ON 4 TMA64 complement(683943..685640) Protein of unknown function that associates with ribosomes; has a putative RNA binding domain YDR118W 1 685879 687837 ON 4 APC4 (685879..687837) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YDR118W-A 1 687765 687881 OFF 4 YDR118W-A (687765..687881) Dubious open reading frame; identified using a combination of expression profiling and mass spectrometry YDR119W 1 688224 690530 ON 4 YDR119W (688224..690530) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuolar membrane; YDR119W is not an essential gene YDR119W-A 1 691011 691211 ON 4 YDR119W-A (691011..691211) Putative protein of unknown function YDR120C 0 691546 693258 ON 4 TRM1 complement(691546..693258) tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments YDR121W 1 693582 694172 ON 4 DPB4 (693582..694172) Shared subunit of DNA polymerase (II) epsilon and of ISW2/yCHRAC chromatin accessibility complex; involved in both chromosomal DNA replication and in inheritance of telomeric silencing YDR122W 1 694697 697891 ON 4 KIN1 (694697..697891) Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p YDR123C 0 698551 699465 ON 4 INO2 complement(698551..699465) Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion YDR124W 1 700312 701286 ON 4 YDR124W (700312..701286) Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor YDR125C 0 701394 702755 ON 4 ECM18 complement(701394..702755) Protein of unknown function, similar to Rlp24p YDR126W 1 703231 704241 ON 4 SWF1 (703231..704241) Palmitoyltransferase that acts on the SNAREs Snc1p, Syn8p, Tlg1p and likely on all SNAREs; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion YDR127W 1 704481 709247 ON 4 ARO1 (704481..709247) Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids YDR128W 1 709546 712992 ON 4 YDR128W (709546..712992) Putative protein of unknown function YDR129C 0 713337 715376 ON 4 SAC6 complement(713337..715244,715356..715376) Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton YDR130C 0 715744 716619 ON 4 FIN1 complement(715744..716619) Spindle pole body-related intermediate filament protein, forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells, able to self-assemble, expression induced during S/G2, localization cell-cycle dependent YDR131C 0 716787 718457 ON 4 YDR131C complement(716787..718457) F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex YDR132C 0 718813 720300 ON 4 YDR132C complement(718813..720300) Putative protein of unknown function YDR133C 0 720959 721294 OFF 4 YDR133C complement(720959..721294) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C YDR134C 0 721068 721478 ON 4 YDR134C complement(721068..721478) Hypothetical protein YDR135C 0 723001 727548 ON 4 YCF1 complement(723001..727548) Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin; similar to human cystic fibrosis protein CFTR YDR136C 0 727708 728280 OFF 4 VPS61 complement(727708..728280) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect YDR137W 1 728256 730247 ON 4 RGP1 (728256..730247) Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p YDR138W 1 730575 732833 ON 4 HPR1 (730575..732833) Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p YDR139C 0 733615 733921 ON 4 RUB1 complement(733615..733699,733773..733921) Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) YDR140W 1 734135 734800 ON 4 MTQ2 (734135..734800) S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; methylates release factor eRF1 (Sup45p) in vitro; is not an essential gene; similar to E.coli PrmC YDR141C 0 734898 739994 ON 4 DOP1 complement(734898..739994) Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR142C 0 740470 741597 ON 4 PEX7 complement(740470..741597) Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) YDR143C 0 742039 743871 ON 4 SAN1 complement(742039..743871) Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear proteins; san1 mutations suppress sir4, spt16, and cdc68 mutations, suggesting a role in chromatin silencing YDR144C 0 744308 746098 ON 4 MKC7 complement(744308..746098) GPI-anchored aspartyl protease (yapsin) involved in protein processing; shares functions with Yap3p and Kex2p YDR145W 1 746735 748354 ON 4 TAF12 (746735..748354) Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A YDR146C 0 748610 750739 ON 4 SWI5 complement(748610..750739) Transcription factor that activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase YDR147W 1 751628 753232 ON 4 EKI1 (751628..753232) Ethanolamine kinase, primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; also exhibits choline kinase activity YDR148C 0 753672 755063 ON 4 KGD2 complement(753672..755063) Dihydrolipoyl transsuccinylase, a component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA YDR149C 0 755552 756259 OFF 4 YDR149C complement(755552..756259) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR150W 1 755625 763871 ON 4 NUM1 (755625..763871) Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex YDR151C 0 764175 765152 ON 4 CTH1 complement(764175..765152) Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis YDR152W 1 765703 766500 ON 4 GIR2 (765703..766500) Highly-acidic cytoplasmic RWD domain-containing protein of unknown function, sensitive to proteolysis, N-terminal region has high content of acidic amino acid residues, putative IUP (intrinsically unstructured protein) YDR153C 0 766733 767968 ON 4 ENT5 complement(766733..767968) Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin YDR154C 0 768400 768750 OFF 4 YDR154C complement(768400..768750) Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein YDR155C 0 768509 768997 ON 4 CPR1 complement(768509..768997) Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A YDR156W 1 769522 769935 ON 4 RPA14 (769522..769935) RNA polymerase I subunit A14 YDR157W 1 769928 770329 OFF 4 YDR157W (769928..770329) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR158W 1 770354 771451 ON 4 HOM2 (770354..771451) Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis YDR159W 1 771874 775779 ON 4 SAC3 (771874..775779) Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus YDR160W 1 776160 778718 ON 4 SSY1 (776160..778718) Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes YDR161W 1 779040 780203 ON 4 YDR161W (779040..780203) Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation YDR162C 0 780387 781097 ON 4 NBP2 complement(780387..781097) Protein involved in the HOG (high osmolarity glycerol) pathway, negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex, found in the nucleus and cytoplasm, contains an SH3 domain that binds Pbs2p YDR163W 1 781420 781947 ON 4 CWC15 (781420..781947) Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p YDR164C 0 782038 784212 ON 4 SEC1 complement(782038..784212) Sm-like protein involved in docking and fusion of exocytic vesicles through binding to assembled SNARE complexes at the membrane; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function YDR165W 1 784868 786202 ON 4 TRM82 (784868..786202) Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA YDR166C 0 786303 789218 ON 4 SEC5 complement(786303..789218) Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YDR167W 1 789446 790066 ON 4 TAF10 (789446..790066) Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification YDR168W 1 790325 791845 ON 4 CDC37 (790325..791845) Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding YDR169C 0 792345 793886 ON 4 STB3 complement(792345..793886) Protein that binds Sin3p in a two-hybrid assay YDR169C-A 0 794571 794720 OFF 4 YDR169C-A complement(794571..794720) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR170C 0 796190 802219 ON 4 SEC7 complement(796190..802219) Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles YDR171W 1 806618 807745 ON 4 HSP42 (806618..807745) Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock YDR172W 1 808321 810378 ON 4 SUP35 (808321..810378) Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype YDR173C 0 810562 811629 ON 4 ARG82 complement(810562..811629) Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; putative nuclear lipid inositol PI3-kinase; regulates arginine-, phosphate-, and nitrogen-responsive genes YDR174W 1 812107 812847 ON 4 HMO1 (812107..812847) Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase YDR175C 0 813190 814149 ON 4 RSM24 complement(813190..814149) Mitochondrial ribosomal protein of the small subunit YDR176W 1 814449 816557 ON 4 NGG1 (814449..816557) Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex YDR177W 1 816875 817522 ON 4 UBC1 (816875..817522) Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response YDR178W 1 817947 818492 ON 4 SDH4 (817947..818492) Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone YDR179C 0 818705 819193 ON 4 CSN9 complement(818705..819193) Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling YDR179W-A 1 819430 820821 ON 4 YDR179W-A (819430..820821) Putative protein of unknown function YDR180W 1 821292 825773 ON 4 SCC2 (821292..825773) Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX YDR181C 0 825907 827352 ON 4 SAS4 complement(825907..827352) Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p YDR182W 1 827579 829054 ON 4 CDC1 (827579..829054) Putative membrane protein of unknown function involved in Mn2+ homeostasis; mutants display actin and general growth defects, heterogeneous cell cycle arrests, and pleiotropic defects in cell cycle progression and organelle distribution YDR182W-A 1 829148 829351 OFF 4 YDR182W-A (829148..829351) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR183W 1 829582 830274 ON 4 PLP1 (829582..830274) Protein with a possible role in folding of beta-tubulin; has weak similarity to phosducins, which are G-protein regulators YDR183C-A 0 830394 830651 OFF 4 YDR183C-A complement(830394..830651) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YDR184C 0 830626 831510 ON 4 ATC1 complement(830626..831510) Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern YDR185C 0 831931 832470 ON 4 YDR185C complement(831931..832470) Mitochondrial protein of unknown function; has similarity to Ups1p, which is involved in regulation of alternative topogenesis of the dynamin-related GTPase Mgm1p YDR186C 0 832856 835489 ON 4 YDR186C complement(832856..835489) Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR187C 0 836225 836743 OFF 4 YDR187C complement(836225..836743) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W YDR188W 1 836418 838058 ON 4 CCT6 (836418..838058) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif YDR189W 1 838389 840389 ON 4 SLY1 (838389..840389) Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex YDR190C 0 840601 841992 ON 4 RVB1 complement(840601..841992) Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family YDR191W 1 842334 843446 ON 4 HST4 (842334..843446) Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism YDR192C 0 843566 844858 ON 4 NUP42 complement(843566..844858) Subunit of the nuclear pore complex (NPC) that localizes exclusively to the cytoplasmic side; involved in RNA export, most likely at a terminal step; interacts with Gle1p YDR193W 1 844551 844949 OFF 4 YDR193W (844551..844949) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR194C 0 845949 847943 ON 4 MSS116 complement(845949..847943) DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing YDR194W-A 1 848068 848220 OFF 4 YDR194W-A (848068..848220) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR195W 1 848596 850197 ON 4 REF2 (848596..850197) RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes YDR196C 0 850271 850996 ON 4 YDR196C complement(850271..850996) Putative dephospho-CoA kinase (DPCK) that catalyzes the final step in Coenzyme A biosynthesis; essential for viability; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR197W 1 851225 852394 ON 4 CBS2 (851225..852394) Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader YDR198C 0 852530 853969 ON 4 RKM2 complement(852530..853969) Ribosomal protein lysine methyltransferase, responsible for epsilon-trimethylation of the lysine residue at position 10 of Rpl12Ap and Rpl12Bp YDR199W 1 854172 854537 OFF 4 YDR199W (854172..854537) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity YDR200C 0 854276 856090 ON 4 VPS64 complement(854276..856090) Cytoplasmic protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p, Far7p, Far10p, and Far11p that is involved in pheromone-induced cell cycle arrest; also localized to the endoplasmic reticulum membrane YDR201W 1 856314 856811 ON 4 SPC19 (856314..856811) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body YDR202C 0 856895 857950 ON 4 RAV2 complement(856895..857950) Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme YDR203W 1 857679 857996 OFF 4 YDR203W (857679..857996) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR204W 1 858134 859141 ON 4 COQ4 (858134..859141) Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex YDR205W 1 859343 861517 ON 4 MSC2 (859343..861517) Member of the cation diffusion facilitator family, localizes to the endoplasmic reticulum and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids YDR206W 1 862051 864705 ON 4 EBS1 (862051..864705) Cytoplasmic protein of unknown function, involved in inhibition of translation; interacts with cap binding protein Cdc33; contains an RNA recognition motif and similarity to Est1p; mRNA abundance regulated by mRNA decay factors YDR207C 0 865009 867519 ON 4 UME6 complement(865009..867519) Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p YDR208W 1 868221 870560 ON 4 MSS4 (868221..870560) Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation YDR209C 0 871033 871446 OFF 4 YDR209C complement(871033..871446) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. YDR210W 1 871071 871298 ON 4 YDR210W (871071..871298) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YDR211W 1 884724 886862 ON 4 GCD6 (884724..886862) Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YDR212W 1 887229 888908 ON 4 TCP1 (887229..888908) Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptide YDR213W 1 889748 892489 ON 4 UPC2 (889748..892489) Sterol regulatory element binding protein, induces transcription of sterol transport and biosynthetic genes; involved in the anaerobic induction of DAN/TIR mannoproteins and seripauperins; binucleate zinc cluster protein; Ecm22p homolog YDR214W 1 892872 893924 ON 4 AHA1 (892872..893924) Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock YDR215C 0 894115 894498 OFF 4 YDR215C complement(894115..894498) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein YDR216W 1 895031 899002 ON 4 ADR1 (895031..899002) Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization YDR217C 0 899547 903476 ON 4 RAD9 complement(899547..903476) DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate YDR218C 0 903777 905048 ON 4 SPR28 complement(903777..905048) Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation YDR219C 0 905451 906848 ON 4 MFB1 complement(905451..906848) Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding YDR220C 0 906996 907289 OFF 4 YDR220C complement(906996..907289) Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein YDR221W 1 907326 909434 ON 4 GTB1 (907326..909434) Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p, involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER YDR222W 1 910050 911297 ON 4 YDR222W (910050..911297) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR223W 1 912095 913498 ON 4 CRF1 (912095..913498) Transcriptional corepressor involved in the regulation of ribosomal protein gene transcription via the TOR signaling pathway and protein kinase A, phosphorylated by activated Yak1p which promotes accumulation of Crf1p in the nucleus YDR224C 0 914313 914708 ON 4 HTB1 complement(914313..914708) One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation YDR225W 1 915526 915924 ON 4 HTA1 (915526..915924) One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p YDR226W 1 916482 917150 ON 4 ADK1 (916482..917150) Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence YDR227W 1 917567 921643 ON 4 SIR4 (917567..921643) Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan YDR228C 0 921922 923802 ON 4 PCF11 complement(921922..923802) mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p) YDR229W 1 924781 926142 ON 4 IVY1 (924781..926142) Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase YDR230W 1 926219 926566 OFF 4 YDR230W (926219..926566) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 YDR231C 0 926289 926906 ON 4 COX20 complement(926289..926906) Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase YDR232W 1 927448 929094 ON 4 HEM1 (927448..929094) 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p YDR233C 0 929466 930353 ON 4 RTN1 complement(929466..930353) ER membrane protein that interacts with exocyst subunit Sec6p and with Yip3p; null mutant has an altered (mostly cisternal) ER morphology; has similarity to mammalian reticulon proteins and member of the RTNLA (reticulon-like A) subfamily YDR234W 1 931125 933206 ON 4 LYS4 (931125..933206) Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway YDR235W 1 933500 935134 ON 4 PRP42 (933500..935134) U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats YDR236C 0 935232 935888 ON 4 FMN1 complement(935232..935888) Riboflavin kinase, phosphorylates riboflavin to form riboflavin monophosphate (FMN), which is a necessary cofactor for many enzymes; localizes to microsomes and to the mitochondrial inner membrane YDR237W 1 936611 937489 ON 4 MRPL7 (936611..937489) Mitochondrial ribosomal protein of the large subunit YDR238C 0 937891 940812 ON 4 SEC26 complement(937891..940812) Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) YDR239C 0 941053 943416 ON 4 YDR239C complement(941053..943416) Protein of unknown function that may interact with ribosomes, based on co-purification experiments YDR240C 0 943670 945148 ON 4 SNU56 complement(943670..945148) Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex YDR241W 1 945147 945434 OFF 4 BUD26 (945147..945434) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay YDR242W 1 946803 948452 ON 4 AMD2 (946803..948452) Putative amidase YDR243C 0 948514 950280 ON 4 PRP28 complement(948514..950280) RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site YDR244W 1 950559 952397 ON 4 PEX5 (950559..952397) Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions YDR245W 1 952796 953977 ON 4 MNN10 (952796..953977) Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family YDR246W 1 954284 954943 ON 4 TRS23 (954284..954943) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 YDR246W-A 1 955129 955329 OFF 4 YDR246W-A (955129..955329) Putative protein of unknown function; identified by fungal homology and RT-PCR YDR247W 1 956009 957394 ON 4 VHS1 (956009..957394) Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p YDR248C 0 957754 958335 ON 4 YDR248C complement(957754..958335) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YDR249C 0 958677 959798 ON 4 YDR249C complement(958677..959798) Putative protein of unknown function YDR250C 0 960082 960357 OFF 4 YDR250C complement(960082..960357) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YDR251W 1 960610 963102 ON 4 PAM1 (960610..963102) Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype YDR252W 1 963408 963857 ON 4 BTT1 (963408..963857) Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex (alpha, beta1, beta3) which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides YDR253C 0 963986 964561 ON 4 MET32 complement(963986..964561) Zinc-finger DNA-binding protein, involved in transcriptional regulation of the methionine biosynthetic genes, similar to Met31p YDR254W 1 965109 966485 ON 4 CHL4 (965109..966485) Outer kinetochore protein required for chromosome stability, interacts with kinetochore proteins Ctf19p, Ctf3p, and Iml3p; exhibits a two-hybrid interaction with Mif2p; association with CEN DNA requires Ctf19p YDR255C 0 966553 967818 ON 4 RMD5 complement(966553..967818) Cytosolic protein required for sporulation; also required for the ubiquitination of the gluconeogenetic enzyme fructose-1,6-bisphosphatase, which is degraded rapidly after the switch from gluconeogenesis to glycolysis YDR256C 0 968129 969676 ON 4 CTA1 complement(968129..969676) Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation YDR257C 0 969986 971470 ON 4 SET7 complement(969986..971470) Nuclear protein that contains a SET-domain, which have been shown to mediate methyltransferase activity in other proteins YDR258C 0 971804 974239 ON 4 HSP78 complement(971804..974239) Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system YDR259C 0 974627 975778 ON 4 YAP6 complement(974627..975778) Putative basic leucine zipper (bZIP) transcription factor; overexpression increases sodium and lithium tolerance YDR260C 0 976713 977225 ON 4 SWM1 complement(976713..977225) Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation YDR261C 0 977517 979205 ON 4 EXG2 complement(977517..979205) Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor YDR262W 1 993130 993948 ON 4 YDR262W (993130..993948) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment YDR263C 0 994238 995530 ON 4 DIN7 complement(994238..995530) Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination YDR264C 0 996025 998319 ON 4 AKR1 complement(996025..998319) Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats YDR265W 1 998860 999873 ON 4 PEX10 (998860..999873) C3HC4-type RING-finger peroxisomal membrane peroxin required for peroxisomal matrix protein import, interacts with Pex12p, links ubiquitin-conjugating Pex4p to import machinery; mutations in human homolog cause various peroxisomal disorders YDR266C 0 1000100 1002019 ON 4 YDR266C complement(1000100..1002019) Protein of unknown function that may interact with ribosomes, based on co-purification experiments;green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; contains a RING finger domain YDR267C 0 1002506 1003498 ON 4 CIA1 complement(1002506..1003498) Essential protein involved in assembly of cytosolic and nuclear iron-sulfur proteins YDR268W 1 1004000 1005139 ON 4 MSW1 (1004000..1005139) Mitochondrial tryptophanyl-tRNA synthetase YDR269C 0 1005661 1005984 OFF 4 YDR269C complement(1005661..1005984) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR270W 1 1005671 1008685 ON 4 CCC2 (1005671..1008685) Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases YDR271C 0 1008391 1008762 OFF 4 YDR271C complement(1008391..1008762) Hypothetical protein YDR272W 1 1009006 1009830 ON 4 GLO2 (1009006..1009830) Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate YDR273W 1 1010172 1011269 ON 4 DON1 (1010172..1011269) Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II YDR274C 0 1011585 1011956 OFF 4 YDR274C complement(1011585..1011956) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR275W 1 1012248 1012955 ON 4 BSC2 (1012248..1012955) Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression YDR276C 0 1013472 1013639 ON 4 PMP3 complement(1013472..1013639) Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential YDR277C 0 1014397 1015698 ON 4 MTH1 complement(1014397..1015698) Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation YDR278C 0 1016997 1017314 OFF 4 YDR278C complement(1016997..1017314) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR279W 1 1019364 1020416 ON 4 RNH202 (1019364..1020416) Ribonuclease H2 subunit, required for RNase H2 activity YDR280W 1 1020743 1021660 ON 4 RRP45 (1020743..1021660) Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex YDR281C 0 1022003 1022317 ON 4 PHM6 complement(1022003..1022317) Protein of unknown function, expression is regulated by phosphate levels YDR282C 0 1023507 1024751 ON 4 YDR282C complement(1023507..1024751) Putative protein of unknown function YDR283C 0 1025066 1030045 ON 4 GCN2 complement(1025066..1030045) Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex YDR284C 0 1030546 1031415 ON 4 DPP1 complement(1030546..1031415) Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism YDR285W 1 1032432 1035059 ON 4 ZIP1 (1032432..1035059) Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate YDR286C 0 1035227 1035571 ON 4 YDR286C complement(1035227..1035571) Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site YDR287W 1 1035991 1036869 ON 4 YDR287W (1035991..1036869) Inositol monophosphatase YDR288W 1 1037191 1038102 ON 4 NSE3 (1037191..1038102) Essential subunit of the Mms21-Smc5-Smc6 complex; protein of unknown function; required for DNA repair and growth YDR289C 0 1038276 1039505 ON 4 RTT103 complement(1038276..1039505) Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition YDR290W 1 1039366 1039695 OFF 4 YDR290W (1039366..1039695) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 YDR291W 1 1039724 1042957 ON 4 YDR291W (1039724..1042957) Putative DNA helicase YDR292C 0 1043142 1045007 ON 4 SRP101 complement(1043142..1045007) Signal recognition particle (SRP) receptor - alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with SRP102p YDR293C 0 1045636 1049388 ON 4 SSD1 complement(1045636..1049388) Protein with a role in maintenance of cellular integrity, interacts with components of the TOR pathway; ssd1 mutant of a clinical S. cerevisiae strain displays elevated virulence YDR294C 0 1050455 1052224 ON 4 DPL1 complement(1050455..1052224) Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate YDR295C 0 1052619 1054643 ON 4 HDA2 complement(1052619..1054643) Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance YDR296W 1 1055208 1055888 ON 4 MHR1 (1055208..1055888) Protein involved in homologous recombination in mitochondria and in transcription regulation in nucleus; binds to activation domains of acidic activators; required for recombination-dependent mtDNA partitioning YDR297W 1 1056547 1057596 ON 4 SUR2 (1056547..1057596) Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis YDR298C 0 1058172 1058810 ON 4 ATP5 complement(1058172..1058810) Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein) YDR299W 1 1059623 1061227 ON 4 BFR2 (1059623..1061227) Essential protein possibly involved in secretion; multicopy suppressor of sensitivity to Brefeldin A YDR300C 0 1061501 1062787 ON 4 PRO1 complement(1061501..1062787) Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis YDR301W 1 1063348 1067421 ON 4 CFT1 (1063348..1067421) RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF YDR302W 1 1067727 1068386 ON 4 GPI11 (1067727..1068386) ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog YDR303C 0 1068725 1071382 ON 4 RSC3 complement(1068725..1071382) Component of the RSC chromatin remodeling complex; essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p YDR304C 0 1071876 1072553 ON 4 CPR5 complement(1071876..1072553) Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER YDR305C 0 1072742 1073484 ON 4 HNT2 complement(1072742..1073308,1073398..1073484) Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins YDR306C 0 1073731 1075167 ON 4 YDR306C complement(1073731..1075167) F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain YDR307W 1 1075861 1077849 ON 4 YDR307W (1075861..1077849) Putative protein of unknown function YDR308C 0 1078023 1078445 ON 4 SRB7 complement(1078023..1078445) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p YDR309C 0 1079044 1080195 ON 4 GIC2 complement(1079044..1080195) Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain YDR310C 0 1081124 1084312 ON 4 SUM1 complement(1081124..1084312) Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; involved in telomere maintenance, regulated by the pachytene checkpoint YDR311W 1 1085062 1086990 ON 4 TFB1 (1085062..1086990) Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators YDR312W 1 1087578 1088939 ON 4 SSF2 (1087578..1088939) Protein required for ribosomal large subunit maturation, functionally redundant with Ssf1p; member of the Brix family YDR313C 0 1089216 1090076 ON 4 PIB1 complement(1089216..1090076) RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain YDR314C 0 1090430 1092508 ON 4 RAD34 complement(1090430..1092508) Protein involved in nucleotide excision repair (NER); homologous to RAD4 YDR315C 0 1092738 1093583 ON 4 IPK1 complement(1092738..1093583) Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable YDR316W 1 1093760 1095175 ON 4 OMS1 (1093760..1095175) Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations YDR317W 1 1102181 1103425 ON 4 HIM1 (1102181..1103425) Protein of unknown function involved in DNA repair YDR318W 1 1103755 1104944 ON 4 MCM21 (1103755..1103806,1103890..1104944) Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules YDR319C 0 1105000 1105824 ON 4 YDR319C complement(1105000..1105824) Putative protein of unknown function; identified as interacting with Sst2p and Hsp82p in high-throughput two-hybrid screens YDR320C 0 1106092 1108098 ON 4 SWA2 complement(1106092..1108098) Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles YDR320C-A 0 1108277 1108495 ON 4 DAD4 complement(1108277..1108495) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YDR320W-B 1 1108481 1108618 OFF 4 YDR320W-B (1108481..1108618) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DAD4/YDR320C-A YDR321W 1 1108699 1109844 ON 4 ASP1 (1108699..1109844) Cytosolic L-asparaginase, involved in asparagine catabolism YDR322W 1 1110586 1111689 ON 4 MRPL35 (1110586..1111689) Mitochondrial ribosomal protein of the large subunit YDR322C-A 0 1112000 1112290 ON 4 TIM11 complement(1112000..1112290) Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric state of ATP synthase YDR323C 0 1112477 1114024 ON 4 PEP7 complement(1112477..1114024) Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance YDR324C 0 1114430 1116760 ON 4 UTP4 complement(1114430..1116760) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YDR325W 1 1117121 1120228 ON 4 YCG1 (1117121..1120228) Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation and for chromatin binding of the condensin complex YDR326C 0 1120604 1124920 ON 4 YSP2 complement(1120604..1124920) Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification YDR327W 1 1125297 1125623 OFF 4 YDR327W (1125297..1125623) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 YDR328C 0 1125429 1126013 ON 4 SKP1 complement(1125429..1126013) Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase YDR329C 0 1126265 1127590 ON 4 PEX3 complement(1126265..1127590) Peroxisomal membrane protein (PMP) required required for the proper localization and stability of PMPs; interacts with Pex19p YDR330W 1 1127867 1129369 ON 4 UBX5 (1127867..1129369) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p YDR331W 1 1129583 1130818 ON 4 GPI8 (1129583..1130818) ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog YDR332W 1 1130996 1133065 ON 4 YDR332W (1130996..1133065) Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci YDR333C 0 1133255 1135426 ON 4 YDR333C complement(1133255..1135426) Putative protein of unknown function YDR334W 1 1135927 1140471 ON 4 SWR1 (1135927..1140471) Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YDR335W 1 1141162 1144836 ON 4 MSN5 (1141162..1144836) Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for export of several proteins including Swi6p, Far1p, and Pho4p; cargo dissociation involves binding to RanGTP YDR336W 1 1145086 1146030 ON 4 YDR336W (1145086..1146030) Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene YDR337W 1 1146313 1147173 ON 4 MRPS28 (1146313..1147173) Mitochondrial ribosomal protein of the small subunit YDR338C 0 1147373 1149460 ON 4 YDR338C complement(1147373..1149460) Hypothetical protein YDR339C 0 1149945 1150514 ON 4 FCF1 complement(1149945..1150514) Essential nucleolar protein that is a component of the SSU (small subunit) processome involved in the pre-rRNA processing steps of 40S ribosomal subunit biogenesis; contains a PINc domain; copurifies with Faf1p YDR340W 1 1150875 1151177 OFF 4 YDR340W (1150875..1151177) Putative protein of unknown function YDR341C 0 1151797 1153620 ON 4 YDR341C complement(1151797..1153620) Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR342C 0 1154210 1155922 ON 4 HXT7 complement(1154210..1155922) High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels YDR343C 0 1159602 1161314 ON 4 HXT6 complement(1159602..1161314) High-affinity glucose transporter of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3 YDR344C 0 1162000 1162443 OFF 4 YDR344C complement(1162000..1162443) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR345C 0 1162951 1164654 ON 4 HXT3 complement(1162951..1164654) Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low or high glucose conditions YDR346C 0 1167208 1168653 ON 4 SVF1 complement(1167208..1168653) Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis YDR347W 1 1169172 1170137 ON 4 MRP1 (1169172..1170137) Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein YDR348C 0 1170320 1171819 ON 4 YDR348C complement(1170320..1171819) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud neck; potential Cdc28p substrate YDR349C 0 1172380 1174170 ON 4 YPS7 complement(1172380..1174170) Putative GPI-anchored aspartic protease, located in the cytoplasm and endoplasmic reticulum YDR350C 0 1176332 1178167 ON 4 ATP22 complement(1176332..1178167) Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YDR351W 1 1178659 1181253 ON 4 SBE2 (1178659..1181253) Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth YDR352W 1 1181794 1182747 ON 4 YDR352W (1181794..1182747) Putative protein of unknown function YDR353W 1 1183292 1184251 ON 4 TRR1 (1183292..1184251) Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress YDR354W 1 1184740 1185882 ON 4 TRP4 (1184740..1185882) Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis YDR354C-A 0 1185132 1185275 OFF 4 YDR354C-A complement(1185132..1185275) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; completely overlaps TRP4; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR355C 0 1186060 1186362 OFF 4 YDR355C complement(1186060..1186362) Hypothetical protein; open reading frame overlaps 5' end of essential SPC110 gene encoding an inner plaque spindle pole body component YDR356W 1 1186100 1188934 ON 4 SPC110 (1186100..1188934) Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner YDR357C 0 1189192 1189560 ON 4 YDR357C complement(1189192..1189560) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR358W 1 1190052 1191725 ON 4 GGA1 (1190052..1191725) Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi YDR359C 0 1191929 1194877 ON 4 EAF1 complement(1191929..1194877) Component of the NuA4 histone acetyltransferase complex YDR360W 1 1194591 1194977 OFF 4 OPI7 (1194591..1194977) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. YDR361C 0 1195404 1196255 ON 4 BCP1 complement(1195404..1196255) Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport YDR362C 0 1196671 1198689 ON 4 TFC6 complement(1196671..1198689) One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 YDR363W 1 1199175 1200545 ON 4 ESC2 (1199175..1200545) Protein involved in mating-type locus silencing, interacts with Sir2p; probably functions to recruit or stabilize Sir proteins YDR363W-A 1 1202120 1202389 ON 4 SEM1 (1202120..1202389) Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; ortholog of human DSS1 YDR364C 0 1202835 1204202 ON 4 CDC40 complement(1202835..1204202) Pre-mRNA splicing factor, important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression; required for DNA synthesis during mitosis and meiosis; has WD repeats YDR365C 0 1204489 1206375 ON 4 ESF1 complement(1204489..1206375) Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels YDR366C 0 1212430 1212828 OFF 4 YDR366C complement(1212430..1212828) Hypothetical protein YDR367W 1 1212840 1213606 ON 4 YDR367W (1212840..1212869,1212971..1213606) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR368W 1 1213896 1214834 ON 4 YPR1 (1213896..1214834) 2-methylbutyraldehyde reductase, may be involved in isoleucine catabolism YDR369C 0 1215008 1217572 ON 4 XRS2 complement(1215008..1217572) Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling YDR370C 0 1217774 1219102 ON 4 YDR370C complement(1217774..1219102) Putative protein of unknown function YDR371W 1 1219405 1220940 ON 4 CTS2 (1219405..1220940) Sporulation-specific chitinase YDR371C-A 0 1219501 1219605 OFF 4 YDR371C-A complement(1219501..1219605) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completeley overlaps the uncharacterized gene CTS2/YDR371W; identified by expression profiling and mass spectrometry YDR372C 0 1221104 1222141 ON 4 VPS74 complement(1221104..1222141) Non-essential protein of unknown function involved in vacuolar protein sorting; belongs to a family of cytosolic Golgi-associated proteins suggesting that it may play a role in secretion; also detected in the nucleus YDR373W 1 1222751 1223323 ON 4 FRQ1 (1222751..1223323) N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily YDR374C 0 1223466 1224386 ON 4 YDR374C complement(1223466..1224386) Putative protein of unknown function YDR374W-A 1 1224749 1225018 ON 4 YDR374W-A (1224749..1225018) Putative protein of unknown function YDR375C 0 1225158 1226528 ON 4 BCS1 complement(1225158..1226528) Protein of the mitochondrial inner membrane that functions as an ATP-dependent chaperone, required for the incorporation of the Rip1p and Qcr10p subunits into the cytochrome bc(1) complex; member of the CDC48/PAS1/SEC18 ATPase family YDR376W 1 1226814 1228295 ON 4 ARH1 (1226814..1228295) Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability YDR377W 1 1228603 1228908 ON 4 ATP17 (1228603..1228908) Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YDR378C 0 1229341 1229601 ON 4 LSM6 complement(1229341..1229601) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YDR379W 1 1230159 1233188 ON 4 RGA2 (1230159..1233188) GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth YDR379C-A 0 1233270 1233509 ON 4 YDR379C-A complement(1233270..1233509) Hypothetical protein identified by homology. See FEBS Letters [2000] 487:31-36. YDR380W 1 1234210 1236117 ON 4 ARO10 (1234210..1236117) Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway YDR381W 1 1236550 1237996 ON 4 YRA1 (1236550..1236834,1237601..1237996) Nuclear protein that binds to RNA and to Mex67p, required for export of poly(A)+ mRNA from the nucleus; member of the REF (RNA and export factor binding proteins) family; another family member, Yra2p, can substitute for Yra1p function YDR381C-A 0 1238304 1238842 ON 4 YDR381C-A complement(1238304..1238622,1238817..1238842) Protein of unknown function, localized to the mitochondrial outer membrane YDR382W 1 1239484 1239816 ON 4 RPP2B (1239484..1239816) Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm YDR383C 0 1239952 1240668 ON 4 NKP1 complement(1239952..1240668) Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) YDR384C 0 1241196 1242023 ON 4 ATO3 complement(1241196..1242023) Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; member of the TC 9.B.33 YaaH family of putative transporters YDR385W 1 1243222 1245750 ON 4 EFT2 (1243222..1245750) Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin YDR386W 1 1246076 1247974 ON 4 MUS81 (1246076..1247974) Helix-hairpin-helix protein, involved in DNA repair and replication fork stability; functions as an endonuclease in complex with Mms4p; interacts with Rad54p YDR387C 0 1248146 1249813 ON 4 YDR387C complement(1248146..1249813) Putative transporter, member of the sugar porter family; YDR387C is not an essential gene YDR388W 1 1250178 1251626 ON 4 RVS167 (1250178..1251626) Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin YDR389W 1 1252529 1254493 ON 4 SAC7 (1252529..1254493) GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate YDR390C 0 1254929 1256839 ON 4 UBA2 complement(1254929..1256839) Nuclear protein that acts as a heterodimer with Aos1p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability YDR391C 0 1257350 1258048 ON 4 YDR391C complement(1257350..1258048) Hypothetical protein YDR392W 1 1258688 1259701 ON 4 SPT3 (1258688..1259701) Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters YDR393W 1 1259893 1261263 ON 4 SHE9 (1259893..1261263) Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex YDR394W 1 1261673 1262959 ON 4 RPT3 (1261673..1262959) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B YDR395W 1 1263316 1266150 ON 4 SXM1 (1263316..1266150) Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 YDR396W 1 1266287 1266787 OFF 4 YDR396W (1266287..1266787) Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex YDR397C 0 1266358 1266890 ON 4 NCB2 complement(1266358..1266761,1266854..1266890) Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta YDR398W 1 1267463 1269394 ON 4 UTP5 (1267463..1269394) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YDR399W 1 1270060 1270725 ON 4 HPT1 (1270060..1270725) Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the formation of both inosine monophosphate and guanosine monophosphate; mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome YDR400W 1 1271055 1272077 ON 4 URH1 (1271055..1272077) Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in recycling pyrimidine deoxy- and ribonucleosides via the pyrimidine salvage pathway YDR401W 1 1272215 1272778 OFF 4 YDR401W (1272215..1272778) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR402C 0 1272226 1273695 ON 4 DIT2 complement(1272226..1273695) N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s YDR403W 1 1274594 1276204 ON 4 DIT1 (1274594..1276204) Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure YDR404C 0 1276646 1277161 ON 4 RPB7 complement(1276646..1277161) RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p YDR405W 1 1277638 1278429 ON 4 MRP20 (1277638..1278429) Mitochondrial ribosomal protein of the large subunit YDR406W 1 1279202 1283791 ON 4 PDR15 (1279202..1283791) Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element YDR406W-A 1 1283791 1284036 OFF 4 YDR406W-A (1283791..1284036) Dubious open reading frame unlikely to encode a functional protein; identified by fungal homology and RT-PCR YDR407C 0 1284061 1287930 ON 4 TRS120 complement(1284061..1287930) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic YDR408C 0 1288207 1288851 ON 4 ADE8 complement(1288207..1288851) Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway YDR409W 1 1289398 1292112 ON 4 SIZ1 (1289398..1292112) SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring YDR410C 0 1292364 1293083 ON 4 STE14 complement(1292364..1293083) Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane YDR411C 0 1293361 1294386 ON 4 DFM1 complement(1293361..1294386) ER localized derlin-like family member involved in ER stress and homeostasis; not involved in ERAD or substrate retrotranslocation; interacts with CDC48; contains four transmembrane domains and two SHP boxes YDR412W 1 1294685 1295392 ON 4 YDR412W (1294685..1295392) Protein required for cell viability YDR413C 0 1294828 1295403 OFF 4 YDR413C complement(1294828..1295403) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing YDR414C 0 1295590 1296678 ON 4 ERD1 complement(1295590..1296678) Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) YDR415C 0 1297029 1298153 ON 4 YDR415C complement(1297029..1298153) Putative protein of unknown function YDR416W 1 1298424 1301003 ON 4 SYF1 (1298424..1301003) Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression; similar to Drosophila crooked neck protein YDR417C 0 1301547 1301918 OFF 4 YDR417C complement(1301547..1301918) Hypothetical protein YDR418W 1 1301608 1302105 ON 4 RPL12B (1301608..1302105) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins YDR419W 1 1303166 1305064 ON 4 RAD30 (1303166..1305064) DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteins YDR420W 1 1306259 1311667 ON 4 HKR1 (1306259..1311667) Serine/threonine rich cell surface protein; osmosensor; involved in the regulation of cell wall beta-1,3 glucan synthesis, bud site selection and hyperosmotic response; overexpression confers resistance to Hansenula mrakii killer toxin HM-1 YDR421W 1 1312032 1314884 ON 4 ARO80 (1312032..1314884) Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids YDR422C 0 1315318 1317765 ON 4 SIP1 complement(1315318..1317765) Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions YDR423C 0 1318038 1319267 ON 4 CAD1 complement(1318038..1319267) AP-1-like bZIP transcriptional activator involved in multiple stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins, binds consensus sequence TTACTAA; 5' UTR contains uORFs YDR424C 0 1319379 1319833 ON 4 DYN2 complement(1319379..1319609,1319690..1319712,1319809..1319833) Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex YDR425W 1 1320056 1321933 ON 4 SNX41 (1320056..1321933) Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; forms a complex with Snx4p and Atg20p YDR426C 0 1321621 1321998 OFF 4 YDR426C complement(1321621..1321998) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 YDR427W 1 1322197 1323378 ON 4 RPN9 (1322197..1323378) Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects YDR428C 0 1323446 1324231 ON 4 YDR428C complement(1323446..1324231) Putative alpha/beta-serine hydrolase belonging to the subfamily of hormone-sensitive lipase-like hydrolases YDR429C 0 1324469 1325293 ON 4 TIF35 complement(1324469..1325293) Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation YDR430C 0 1325493 1328462 ON 4 CYM1 complement(1325493..1328462) Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology YDR431W 1 1328382 1328693 OFF 4 YDR431W (1328382..1328693) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR432W 1 1328775 1330019 ON 4 NPL3 (1328775..1330019) RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; dissociation from mRNAs is promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm YDR433W 1 1329592 1330032 OFF 4 YDR433W (1329592..1330032) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR434W 1 1331229 1332833 ON 4 GPI17 (1331229..1332833) Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Sp homolog YDR435C 0 1332975 1333961 ON 4 PPM1 complement(1332975..1333961) Carboxyl methyl transferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits YDR436W 1 1334813 1336945 ON 4 PPZ2 (1334813..1336945) Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance YDR437W 1 1337344 1337766 ON 4 GPI19 (1337344..1337766) Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P YDR438W 1 1338266 1339378 ON 4 THI74 (1338266..1339378) Mitochondrial transporter repressible by thiamine YDR439W 1 1339668 1340711 ON 4 LRS4 (1339668..1340711) Protein involved in rDNA silencing; positively charged coiled-coil protein with limited similarity to myosin YDR440W 1 1342485 1344233 ON 4 DOT1 (1342485..1344233) Nucleosomal histone H3-Lys79 methylase, associates with transcriptionally active genes, functions in gene silencing at telomeres, most likely by directly modulating chromatin structure and Sir protein localization YDR441C 0 1344509 1345054 ON 4 APT2 complement(1344509..1345054) Apparent pseudogene, not transcribed or translated under normal conditions; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity YDR442W 1 1345639 1346031 OFF 4 YDR442W (1345639..1346031) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR443C 0 1345668 1349930 ON 4 SSN2 complement(1345668..1349930) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation YDR444W 1 1350282 1352345 ON 4 YDR444W (1350282..1352345) Putative protein of unknown function YDR445C 0 1352174 1352581 OFF 4 YDR445C complement(1352174..1352581) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR446W 1 1353717 1354625 ON 4 ECM11 (1353717..1354625) Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure YDR447C 0 1354821 1355545 ON 4 RPS17B complement(1354821..1355228,1355543..1355545) Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similarity to rat S17 ribosomal protein YDR448W 1 1356057 1357361 ON 4 ADA2 (1356057..1357361) Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes YDR449C 0 1357572 1358894 ON 4 UTP6 complement(1357572..1358894) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YDR450W 1 1359915 1360790 ON 4 RPS18A (1359915..1359961,1360397..1360790) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Bp and has similarity to E. coli S13 and rat S18 ribosomal proteins YDR451C 0 1361112 1362173 ON 4 YHP1 complement(1361112..1362173) One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval YDR452W 1 1362870 1364894 ON 4 PPN1 (1362870..1364894) Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer YDR453C 0 1365064 1365654 ON 4 TSA2 complement(1365064..1365654) Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants YDR454C 0 1366256 1366819 ON 4 GUK1 complement(1366256..1366819) Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins YDR455C 0 1367365 1367673 OFF 4 YDR455C complement(1367365..1367673) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W YDR456W 1 1367477 1369378 ON 4 NHX1 (1367477..1369378) Endosomal Na+/H+ exchanger, required for intracellular sequestration of Na+; required for osmotolerance to acute hypertonic shock YDR457W 1 1369782 1379588 ON 4 TOM1 (1369782..1379588) E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators YDR458C 0 1380047 1382038 ON 4 YDR458C complement(1380047..1382038) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery YDR459C 0 1382311 1383435 ON 4 PFA5 complement(1382311..1383435) Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain YDR460W 1 1383803 1384768 ON 4 TFB3 (1383803..1384768) Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit YDR461W 1 1385168 1385278 ON 4 MFA1 (1385168..1385278) Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 YDR461C-A 0 1385516 1385758 ON 4 YDR461C-A complement(1385516..1385758) Putative protein of unknown function YDR462W 1 1386065 1386508 ON 4 MRPL28 (1386065..1386508) Mitochondrial ribosomal protein of the large subunit YDR463W 1 1386808 1388367 ON 4 STP1 (1386808..1388367) Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing YDR464W 1 1388864 1393171 ON 4 SPP41 (1388864..1393171) Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 YDR464C-A 0 1392484 1392672 OFF 4 YDR464C-A complement(1392484..1392672) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR465C 0 1393328 1394566 ON 4 RMT2 complement(1393328..1394566) Arginine methyltransferase; ribosomal protein L12 is a substrate YDR466W 1 1395113 1397809 ON 4 PKH3 (1395113..1397809) Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant YDR467C 0 1397575 1397901 OFF 4 YDR467C complement(1397575..1397901) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR468C 0 1398018 1398692 ON 4 TLG1 complement(1398018..1398692) Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p YDR469W 1 1399007 1399534 ON 4 SDC1 (1399007..1399534) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; has similarity to C. elegans Dpy-30 YDR470C 0 1399698 1401206 ON 4 UGO1 complement(1399698..1401206) Protein of unknown function; outer membrane component of the mitochondrial fusion machinery; Ugo1p bind directly to Fzo1p and Mgm1p and thereby link these two GTPases during mitochondrial fusion YDR471W 1 1401762 1402556 ON 4 RPL27B (1401762..1401792,1402177..1402556) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Ap and has similarity to rat L27 ribosomal protein YDR472W 1 1403314 1404165 ON 4 TRS31 (1403314..1404165) One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic YDR473C 0 1404437 1405846 ON 4 PRP3 complement(1404437..1405846) Splicing factor, component of the U4/U6-U5 snRNP complex YDR475C 0 1407456 1410086 ON 4 JIP4 complement(1407456..1410086) Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence YDR476C 0 1410445 1411119 ON 4 YDR476C complement(1410445..1411119) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene YDR477W 1 1412365 1414266 ON 4 SNF1 (1412365..1414266) AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis YDR478W 1 1414567 1415163 ON 4 SNM1 (1414567..1415163) Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP YDR479C 0 1415202 1416866 ON 4 PEX29 complement(1415202..1416866) Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p YDR480W 1 1417391 1418362 ON 4 DIG2 (1417391..1418362) Regulatory protein of unknown function, pheromone-inducible, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription YDR481C 0 1418542 1420242 ON 4 PHO8 complement(1418542..1420242) Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of inorganic phosphate and by a system consisting of Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides YDR482C 0 1420423 1420830 ON 4 CWC21 complement(1420423..1420830) Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; may bind RNA; has similarity to S. pombe Cwf21p YDR483W 1 1421149 1422477 ON 4 KRE2 (1421149..1422477) Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation YDR484W 1 1422755 1424680 ON 4 VPS52 (1422755..1424680) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin YDR485C 0 1424812 1427199 ON 4 VPS72 complement(1424812..1427199) Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting YDR486C 0 1427423 1428112 ON 4 VPS60 complement(1427423..1428112) Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting specific cargo proteins for degradation YDR487C 0 1428346 1428972 ON 4 RIB3 complement(1428346..1428972) 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration YDR488C 0 1429180 1430781 ON 4 PAC11 complement(1429180..1430781) Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 YDR489W 1 1431004 1431888 ON 4 SLD5 (1431004..1431888) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YDR490C 0 1431960 1434260 ON 4 PKH1 complement(1431960..1434260) Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p YDR491C 0 1434492 1434983 OFF 4 YDR491C complement(1434492..1434983) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR492W 1 1434916 1435866 ON 4 IZH1 (1434916..1435866) Membrane protein involved in zinc metabolism, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc YDR493W 1 1436209 1436580 ON 4 YDR493W (1436209..1436580) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YDR494W 1 1436922 1438007 ON 4 RSM28 (1436922..1438007) Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation YDR495C 0 1438107 1441142 ON 4 VPS3 complement(1438107..1441142) Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase YDR496C 0 1441425 1443395 ON 4 PUF6 complement(1441425..1443395) Pumilio-homology domain protein that binds ASH1 mRNA at PUF consensus sequences in the 3' UTR and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA YDR497C 0 1443705 1445459 ON 4 ITR1 complement(1443705..1445459) Myo-inositol transporter with strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p YDR498C 0 1445835 1446986 ON 4 SEC20 complement(1445835..1446986) Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; forms a complex with the cytosolic Tip20p YDR499W 1 1447822 1450065 ON 4 LCD1 (1447822..1450065) Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP YDR500C 0 1450190 1450845 ON 4 RPL37B complement(1450190..1450449,1450839..1450845) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Ap and to rat L37 ribosomal protein YDR501W 1 1451345 1452910 ON 4 PLM2 (1451345..1452910) Protein required for partitioning of the 2-micron plasmid YDR502C 0 1453302 1454456 ON 4 SAM2 complement(1453302..1454456) S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) YDR503C 0 1455034 1455858 ON 4 LPP1 complement(1455034..1455858) Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA YDR504C 0 1456303 1456686 OFF 4 SPG3 complement(1456303..1456686) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources YDR505C 0 1456687 1459212 ON 4 PSP1 complement(1456687..1459212) Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition YDR506C 0 1459720 1461546 ON 4 YDR506C complement(1459720..1461546) Possible membrane-localized protein YDR507C 0 1462350 1465778 ON 4 GIN4 complement(1462350..1465778) Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p YDR508C 0 1466445 1468436 ON 4 GNP1 complement(1466445..1468436) High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids YDR509W 1 1468218 1468565 OFF 4 YDR509W (1468218..1468565) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR510W 1 1469392 1469697 ON 4 SMT3 (1469392..1469697) Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics YDR510C-A 0 1469687 1469803 OFF 4 YDR510C-A complement(1469687..1469803) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR511W 1 1470009 1470410 ON 4 ACN9 (1470009..1470410) Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes YDR512C 0 1470492 1471055 ON 4 EMI1 complement(1470492..1471055) Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation YDR513W 1 1471009 1471440 ON 4 GRX2 (1471009..1471440) Cytoplasmic glutaredoxin, thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress YDR514C 0 1471540 1472991 ON 4 YDR514C complement(1471540..1472991) Putative protein of unknown function YDR515W 1 1473421 1474764 ON 4 SLF1 (1473421..1474764) RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts YDR516C 0 1474966 1476468 ON 4 EMI2 complement(1474966..1476468) Non-essential protein of unknown function required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression is regulated by glucose-repression transcription factors Mig1/2p YDR517W 1 1477231 1478349 ON 4 GRH1 (1477231..1478349) Acetylated, cis-golgi localized protein involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes YDR518W 1 1478600 1480153 ON 4 EUG1 (1478600..1480153) Protein disulfide isomerase of the endoplasmic reticulum lumen, function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER YDR519W 1 1480417 1480824 ON 4 FPR2 (1480417..1480824) Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking YDR520C 0 1481077 1483395 ON 4 YDR520C complement(1481077..1483395) Putative transcription factor; contains the (Zn(II)2Cys6 motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is viable and sensitive to caffeine YDR521W 1 1483133 1483468 OFF 4 YDR521W (1483133..1483468) Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol YDR522C 0 1483787 1485295 ON 4 SPS2 complement(1483787..1485295) Protein expressed during sporulation, redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component YDR523C 0 1485558 1487030 ON 4 SPS1 complement(1485558..1487030) Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis YDR524C 0 1487534 1488982 ON 4 AGE1 complement(1487534..1488982) ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif YDR524W-C 1 1489395 1489484 OFF 4 YDR524W-C (1489395..1489484) Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin YDR524C-B 0 1489590 1489790 ON 4 YDR524C-B complement(1489590..1489790) Putative protein of unknown function YDR524C-A 0 1489863 1489982 OFF 4 YDR524C-A complement(1489863..1489982) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YDR525W 1 1489897 1490226 OFF 4 API2 (1489897..1490226) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology YDR525W-A 1 1490588 1490827 ON 4 SNA2 (1490588..1490827) Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YDR526C 0 1491067 1491537 OFF 4 YDR526C complement(1491067..1491537) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR527W 1 1491086 1492405 ON 4 RBA50 (1491086..1492405) Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 YDR528W 1 1494578 1495849 ON 4 HLR1 (1494578..1495849) Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A YDR529C 0 1496157 1496540 ON 4 QCR7 complement(1496157..1496540) Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly YDR530C 0 1496783 1497760 ON 4 APA2 complement(1496783..1497760) Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase II (AP4A phosphorylase), involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; has similarity to Apa1p YDR531W 1 1498224 1499327 ON 4 YDR531W (1498224..1499327) Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway leading to coenzyme A1 YDR532C 0 1499388 1500545 ON 4 YDR532C complement(1499388..1500545) Protein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p YDR533C 0 1501439 1502152 ON 4 HSP31 complement(1501439..1502152) Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer YDR534C 0 1503305 1504891 ON 4 FIT1 complement(1503305..1504891) Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall YDR535C 0 1506597 1507353 OFF 4 YDR535C complement(1506597..1507049,1507306..1507353) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. YDR536W 1 1507996 1509705 ON 4 STL1 (1507996..1509705) Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock YDR537C 0 1510846 1511451 OFF 4 YDR537C complement(1510846..1511451) Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W YDR538W 1 1510892 1511620 ON 4 PAD1 (1510892..1511620) Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives YDR539W 1 1512084 1513595 ON 4 YDR539W (1512084..1513595) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR539W is not an essential gene YDR540C 0 1517120 1517659 ON 4 YDR540C complement(1517120..1517659) Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci YDR541C 0 1519648 1520682 ON 4 YDR541C complement(1519648..1520682) Putative dihydrokaempferol 4-reductase YDR542W 1 1523234 1523596 ON 4 YDR542W (1523234..1523596) Hypothetical protein YDR543C 0 1524619 1524918 OFF 4 YDR543C complement(1524619..1524918) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR544C 0 1525080 1525508 OFF 4 YDR544C complement(1525080..1525508) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YDR545W 1 1526306 1531696 ON 4 YRF1-1 (1526306..1531696) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YDR545C-A 0 1530848 1531327 OFF 4 YDR545C-A complement(1530848..1531327) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL077C 0 264 4097 ON 5 YEL077C complement(264..4097) Helicase-like protein encoded within the telomeric Y' element YEL077W-A 1 630 1112 OFF 5 YEL077W-A (630..1112) Dubious open reading frame, unlike to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL076C-A 0 4185 5114 ON 5 YEL076C-A complement(4185..4322,4602..5114) Putative protein of unknown function YEL076C 0 4464 5114 ON 5 YEL076C complement(4464..5114) Putative protein of unknown function YEL075W-A 1 4870 5481 OFF 5 YEL075W-A (4870..5481) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL075C 0 5345 5713 ON 5 YEL075C complement(5345..5713) Putative protein of unknown function YEL074W 1 6126 6464 OFF 5 YEL074W (6126..6464) Hypothetical protein YEL073C 0 7230 7553 ON 5 YEL073C complement(7230..7553) Putative protein of unknown function; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline YEL072W 1 13720 14415 ON 5 RMD6 (13720..14415) Protein required for sporulation YEL071W 1 16355 17845 ON 5 DLD3 (16355..17845) D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm YEL070W 1 19589 21097 ON 5 DSF1 (19589..21097) Deletion suppressor of mpt5 mutation YEL069C 0 21537 23231 ON 5 HXT13 complement(21537..23231) Hexose transporter, induced in the presence of non-fermentable carbon sources, induced by low levels of glucose, repressed by high levels of glucose YEL068C 0 25646 25978 OFF 5 YEL068C complement(25646..25978) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL067C 0 26189 26776 OFF 5 YEL067C complement(26189..26776) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YEL066W 1 26667 27206 ON 5 HPA3 (26667..27206) D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro YEL065W 1 27657 29543 ON 5 SIT1 (27657..29543) Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p YEL064C 0 29797 31239 ON 5 AVT2 complement(29797..31239) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YEL063C 0 31694 33466 ON 5 CAN1 complement(31694..33466) Plasma membrane arginine permease, requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance YEL062W 1 34407 36254 ON 5 NPR2 (34407..36254) Protein with a possible role in regulating expression of nitrogen permeases; transcription is induced in response to proline and urea; contains two PEST sequences; null mutant is resistant to cisplatin and doxorubicin YEL061C 0 36535 39537 ON 5 CIN8 complement(36535..39537) Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation YEL060C 0 40046 41953 ON 5 PRB1 complement(40046..41953) Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation YEL059C-A 0 42400 42624 ON 5 SOM1 complement(42400..42624) Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates YEL059W 1 42652 42960 OFF 5 YEL059W (42652..42960) Dubious open reading frame unlikely to encode a functional protein YEL058W 1 43252 44925 ON 5 PCM1 (43252..44925) Essential N-acetylglucosamine-phosphate mutase, a hexosephosphate mutase involved in the biosynthesis of chitin YEL057C 0 45020 45721 ON 5 YEL057C complement(45020..45721) Protein of unknown function involved in telomere maintenance; target of UME6 regulation YEL056W 1 47168 48373 ON 5 HAT2 (47168..48373) Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing YEL055C 0 48471 51539 ON 5 POL5 complement(48471..51539) DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA YEL054C 0 52721 53218 ON 5 RPL12A complement(52721..53218) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Bp; rpl12a rpl12b double mutant exhibits slow growth and slow translation; has similarity to E. coli L11 and rat L12 ribosomal proteins YEL053W-A 1 52908 53255 OFF 5 YEL053W-A (52908..53255) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YEL054C YEL053C 0 53901 56102 ON 5 MAK10 complement(53901..56102) Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible YEL052W 1 56571 58100 ON 5 AFG1 (56571..58100) Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain YEL051W 1 58378 59148 ON 5 VMA8 (58378..59148) Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis YEL050W-A 1 59549 59740 OFF 5 YEL050W-A (59549..59740) Dubious open reading frame unlikely to encode a functional protein, identified by fungal homology and RT-PCR YEL050C 0 59670 60851 ON 5 RML2 complement(59670..60851) Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor YEL049W 1 63728 64090 ON 5 PAU2 (63728..64090) Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YEL048C 0 64709 65167 ON 5 YEL048C complement(64709..65167) Putative protein of unknown function; synthetic lethal with gcs1delta YEL047C 0 65385 66797 ON 5 YEL047C complement(65385..66797) Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies YEL046C 0 67629 68792 ON 5 GLY1 complement(67629..68792) Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis YEL045C 0 68840 69265 OFF 5 YEL045C complement(68840..69265) Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress YEL044W 1 69757 70257 ON 5 IES6 (69757..70257) Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions YEL043W 1 70478 73348 ON 5 YEL043W (70478..73348) Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold YEL042W 1 73771 75327 ON 5 GDA1 (73771..75327) Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate YEL041W 1 75944 77431 ON 5 YEF1 (75944..77431) ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes YEL040W 1 78053 79456 ON 5 UTR2 (78053..79456) Cell wall protein that functions in the transfer of chitin to beta(1-6)glucan; putative chitin transglycosidase; glycosylphosphatidylinositol (GPI)-anchored protein localized to the bud neck; has a role in cell wall maintenance YEL039C 0 79636 79977 ON 5 CYC7 complement(79636..79977) Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration YEL038W 1 80462 81145 ON 5 UTR4 (80462..81145) Protein of unknown function with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, found in both the cytoplasm and nucleus YEL037C 0 81407 82603 ON 5 RAD23 complement(81407..82603) Protein with ubiquitin-like N terminus, recognizes and binds damaged DNA (with Rad4p) during nucleotide excision repair; regulates Rad4p levels, subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human HR23A and HR23B proteins YEL036C 0 83050 84552 ON 5 ANP1 complement(83050..84552) Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol YEL035C 0 85045 85545 OFF 5 UTR5 complement(85045..85545) Protein of unknown function; transcription may be regulated by Gcr1p YEL034C-A 0 85613 86215 OFF 5 YEL034C-A complement(85613..86215) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; completely overlaps HYP2/YEL034W, a verified gene that encodes eiF-5A YEL034W 1 85676 86149 ON 5 HYP2 (85676..86149) Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Anb1p; possible role in translation elongation; undergoes an essential hypusination modification YEL033W 1 86179 86598 OFF 5 YEL033W (86179..86598) Predicted to have metabolic role based on analysis of gene networks derived from chromatin immunoprecipitation experiments, experiments on gene deletion mutants, and localization of transcription factor binding sites YEL032W 1 86937 89852 ON 5 MCM3 (86937..89852) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex YEL032C-A 0 87040 87201 OFF 5 YEL032C-A complement(87040..87201) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL031W 1 90258 93905 ON 5 SPF1 (90258..93905) P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 YEL030C-A 0 94583 94897 OFF 5 YEL030C-A complement(94583..94897) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL030W 1 94644 96578 ON 5 ECM10 (94644..96578) Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations YEL029C 0 96858 97796 ON 5 BUD16 complement(96858..97796) Protein involved in bud-site selection and telomere maintenance; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; has similarity to pyridoxal kinases YEL028W 1 98668 99129 OFF 5 YEL028W (98668..99129) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YEL027W 1 100769 101251 ON 5 CUP5 (100769..101251) Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis YEL026W 1 101943 102323 ON 5 SNU13 (101943..102323) RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein YEL025C 0 102581 106147 ON 5 YEL025C complement(102581..106147) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YEL024W 1 107260 107907 ON 5 RIP1 (107260..107907) Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration YEL023C 0 108504 110552 ON 5 YEL023C complement(108504..110552) Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene YEL022W 1 111421 115800 ON 5 GEA2 (111421..115800) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p YEL021W 1 116167 116970 ON 5 URA3 (116167..116970) Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound YEL020C-B 0 117183 117380 OFF 5 YEL020C-B complement(117183..117380) Dubious open reading frame unlikely to encode a protein; partially overlaps verified gene YEL020W-A; identified by fungal homology and RT-PCR YEL020W-A 1 117211 117474 ON 5 TIM9 (117211..117474) Mitochondrial intermembrane space protein, forms a complex with Mrs11p/Tim10p that mediates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner YEL020C 0 118617 120299 ON 5 YEL020C complement(118617..120299) Hypothetical protein with low sequence identity to Pdc1p YEL019C 0 120498 121301 ON 5 MMS21 complement(120498..121301) SUMO ligase involved in chromosomal organization and DNA repair; essential subunit of the Mms21-Smc5-Smc6 complex; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination YEL018C-A 0 121455 121988 OFF 5 YEL018C-A complement(121455..121988) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL018W 1 121471 122310 ON 5 EAF5 (121471..122310) Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex YEL017C-A 0 122798 122929 ON 5 PMP2 complement(122798..122929) Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; nearly identical to PMP1 YEL017W 1 123657 124670 ON 5 GTT3 (123657..124670) Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery YEL016C 0 124737 126218 ON 5 NPP2 complement(124737..126218) Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation YEL015W 1 126629 128284 ON 5 EDC3 (126629..128284) Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies YEL014C 0 128303 128608 OFF 5 YEL014C complement(128303..128608) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL013W 1 128825 130561 ON 5 VAC8 (128825..130561) Phosphorylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions YEL012W 1 131772 132551 ON 5 UBC8 (131772..131776,131900..132551) Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro YEL011W 1 133120 135234 ON 5 GLC3 (133120..135234) Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YEL010W 1 136279 136629 OFF 5 YEL010W (136279..136629) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL009C-A 0 136371 136778 OFF 5 YEL009C-A complement(136371..136778) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YEL009C 0 138918 139763 ON 5 GCN4 complement(138918..139763) Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels YEL008W 1 140512 140892 OFF 5 YEL008W (140512..140892) Hypothetical protein predicted to be involved in metabolism YEL008C-A 0 140726 140818 OFF 5 YEL008C-A complement(140726..140818) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YEL007W 1 141891 143891 ON 5 YEL007W (141891..143891) Putative protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) YEL006W 1 144326 145333 ON 5 YEA6 (144326..145333) Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog YEL005C 0 145906 146754 ON 5 VAB2 complement(145906..146754) Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YEL004W 1 146950 147978 ON 5 YEA4 (146950..147978) Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER YEL003W 1 148175 148598 ON 5 GIM4 (148175..148193,148282..148598) Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it YEL002C 0 148721 150013 ON 5 WBP1 complement(148721..150013) Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum YEL001C 0 150300 150977 ON 5 YEL001C complement(150300..150977) Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52 foci YER001W 1 153519 155807 ON 5 MNN1 (153519..155807) Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family YER002W 1 156802 157497 ON 5 NOP16 (156802..157497) Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis YER003C 0 157735 159117 ON 5 PMI40 complement(157735..158993,159087..159117) Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation YER004W 1 159579 160274 ON 5 YER004W (159579..160274) Protein of unknown function, localized to the mitochondrial outer membrane YER005W 1 160549 162441 ON 5 YND1 (160549..162441) Apyrase with wide substrate specificity, involved in preventing the inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates which are inhibitors of glycotransferases; partially redundant with Gda1p YER006W 1 162722 164284 ON 5 NUG1 (162722..164284) GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus YER006C-A 0 164343 164660 OFF 5 YER006C-A complement(164343..164660) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PAC2 YER007W 1 164526 166082 ON 5 PAC2 (164526..166082) Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl YER007C-A 0 166236 166884 ON 5 TMA20 complement(166236..166770,166874..166884) Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1 YER008C 0 167807 171817 ON 5 SEC3 complement(167807..171817) Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion YER009W 1 172114 172491 ON 5 NTF2 (172114..172491) Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport YER010C 0 172633 173337 ON 5 YER010C complement(172633..173337) Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction YER011W 1 175247 176011 ON 5 TIR1 (175247..176011) Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking YER012W 1 177834 178430 ON 5 PRE1 (177834..178430) 20S proteasome beta-type subunit; localizes to the nucleus throughout the cell cycle YER013W 1 178840 182277 ON 5 PRP22 (178840..182277) DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes YER014W 1 182599 184218 ON 5 HEM14 (182599..184218) Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX YER014C-A 0 183730 184699 OFF 5 BUD25 complement(183730..184168,184677..184699) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YER015W 1 184540 186774 ON 5 FAA2 (184540..186774) Long chain fatty acyl-CoA synthetase; accepts a wider range of acyl chain lengths than Faa1p, preferring C9:0-C13:0; involved in the activation of endogenous pools of fatty acids YER016W 1 188276 189310 ON 5 BIM1 (188276..189310) Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally YER017C 0 189502 191787 ON 5 AFG3 complement(189502..191787) Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes YER018C 0 191958 192623 ON 5 SPC25 complement(191958..192623) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering YER019W 1 192796 194229 ON 5 ISC1 (192796..194229) Inositol phosphosphingolipid phospholipase C, hydrolyzes inositolphosphosphingolipids, activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol, mediates Na+ and Li+ halotolerance, contains a P loop-like domain YER019C-A 0 194272 194538 ON 5 SBH2 complement(194272..194538) Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p YER020W 1 195167 196516 ON 5 GPA2 (195167..196516) Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YER021W 1 196947 198518 ON 5 RPN3 (196947..198518) Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control YER022W 1 198811 200874 ON 5 SRB4 (198811..200874) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YER023W 1 201075 201935 ON 5 PRO3 (201075..201935) Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis YER023C-A 0 201521 201733 OFF 5 YER023C-A complement(201521..201733) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene PRO3; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YER024W 1 202191 204962 ON 5 YAT2 (202191..204962) Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane YER025W 1 205250 206833 ON 5 GCD11 (205250..206833) Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met YER026C 0 207643 208473 ON 5 CHO1 complement(207643..208473) Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline YER027C 0 208978 210231 ON 5 GAL83 complement(208978..210231) One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain YER028C 0 210691 211875 ON 5 MIG3 complement(210691..211875) Probable transcriptional repressor involved in response to toxic agents such as hydroxyurea that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes YER029C 0 212586 213176 ON 5 SMB1 complement(212586..213176) Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B' YER030W 1 213436 213897 ON 5 CHZ1 (213436..213897) Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif YER031C 0 214075 214746 ON 5 YPT31 complement(214075..214746) GTPase of the Ypt/Rab family, very similar to Ypt32p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi YER032W 1 215062 217692 ON 5 FIR1 (215062..217692) Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate YER033C 0 218056 221286 ON 5 ZRG8 complement(218056..221286) Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency YER034W 1 221845 222402 ON 5 YER034W (221845..222402) Putative protein of unknown function; non-essential gene; expression induced upon calcium shortage YER035W 1 222638 223075 ON 5 EDC2 (222638..223075) RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p YER036C 0 223366 225198 ON 5 ARB1 complement(223366..225198) ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p YER037W 1 225888 226853 ON 5 PHM8 (225888..226853) Protein of unknown function, expression is induced by low phosphate levels and by inactivation of Pho85p YER038C 0 226857 228251 ON 5 KRE29 complement(226857..228251) Essential subunit of the Mms21-Smc5-Smc6 complex; protein of unknown function; required for growth and DNA repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance YER038W-A 1 228450 228830 OFF 5 YER038W-A (228450..228830) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria YER039C 0 228455 229204 ON 5 HVG1 complement(228455..229204) Protein of unknown function, has homology to Vrg4p YER039C-A 0 229262 229480 OFF 5 YER039C-A complement(229262..229480) Putative protein of unknown function; YER039C-A is not an essential gene YER040W 1 229794 231986 ON 5 GLN3 (229794..231986) Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source YER041W 1 232460 234739 ON 5 YEN1 (232460..234739) Protein of unknown function, has similarity to endonuclease Rth1p; potentially phosphorylated by Cdc28p YER042W 1 234936 235490 ON 5 MXR1 (234936..235490) Peptide methionine sulfoxide reductase, reverses the oxidation of methionine residues; involved in oxidative damage repair, providing resistance to oxidative stress and regulation of lifespan YER043C 0 235769 237118 ON 5 SAH1 complement(235769..237118) S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor YER044C 0 237569 238015 ON 5 ERG28 complement(237569..238015) Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p YER044C-A 0 238459 239773 ON 5 MEI4 complement(238459..239622,239711..239773) Meiosis-specific protein involved in recombination; required for chromosome synapsis; required for production of viable spores YER045C 0 240031 241500 ON 5 ACA1 complement(240031..241500) Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, may regulate transcription of genes involved in utilization of non-optimal carbon sources YER046W 1 243179 243610 ON 5 SPO73 (243179..243610) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis YER046W-A 1 243699 244028 OFF 5 YER046W-A (243699..244028) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER047C 0 243809 246502 ON 5 SAP1 complement(243809..246502) Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system YER048C 0 246981 248156 ON 5 CAJ1 complement(246981..248156) Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly YER048W-A 1 250717 251001 ON 5 ISD11 (250717..251001) Protein required for mitochondrial iron-sulfur cluster biosynthesis YER049W 1 251727 253661 ON 5 TPA1 (251727..253661) Protein of unknown function; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; has a role in translation termination efficiency, mRNA poly(A) tail length and mRNA stability YER050C 0 253970 254386 ON 5 RSM18 complement(253970..254386) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein YER051W 1 254655 256133 ON 5 JHD1 (254655..256133) JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe YER052C 0 256374 257957 ON 5 HOM3 complement(256374..257957) Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis YER053C 0 258736 259638 ON 5 PIC2 complement(258736..259638) Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature YER053C-A 0 260932 261045 ON 5 YER053C-A complement(260932..261045) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YER054C 0 262051 263697 ON 5 GIP2 complement(262051..263697) Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p YER055C 0 264891 265784 ON 5 HIS1 complement(264891..265784) ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control YER056C 0 266511 268112 ON 5 FCY2 complement(266511..268112) Purine-cytosine permease, mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation YER056C-A 0 269421 270183 ON 5 RPL34A complement(269421..269749,270147..270183) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Bp and has similarity to rat L34 ribosomal protein YER057C 0 270735 271124 ON 5 HMF1 complement(270735..271124) Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro YER058W 1 271766 272089 ON 5 PET117 (271766..272089) Protein required for assembly of cytochrome c oxidase YER059W 1 272622 273884 ON 5 PCL6 (272622..273884) Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding YER060W 1 274565 276151 ON 5 FCY21 (274565..276151) Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function YER060W-A 1 276570 278162 ON 5 FCY22 (276570..278162) Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function YER061C 0 278296 279624 ON 5 CEM1 complement(278296..279624) Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration YER062C 0 279928 280680 ON 5 HOR2 complement(279928..280680) One of two redundant DL-glycerol-3-phosphatases (RHR2/GPP1 encodes the other) involved in glycerol biosynthesis; induced in response to hyperosmotic stress and oxidative stress, and during the diauxic transition YER063W 1 281708 282364 ON 5 THO1 (281708..282364) Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation YER064C 0 282703 284220 ON 5 YER064C complement(282703..284220) Non-essential nuclear protein; null mutation has global effects on transcription YER065C 0 285239 286912 ON 5 ICL1 complement(285239..286912) Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose YER066W 1 290240 290797 ON 5 YER066W (290240..290797) Putative protein of unknown function; YER066W is not an essential gene YER066C-A 0 291701 292201 OFF 5 YER066C-A complement(291701..292201) Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W YER067W 1 292064 292549 ON 5 YER067W (292064..292549) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YER067W is not an essential gene YER067C-A 0 292238 292561 OFF 5 YER067C-A complement(292238..292561) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W YER068W 1 293048 294811 ON 5 MOT2 (293048..294811) Component of the CCR4-NOT complex, which has multiple roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; with Ubc4p, ubiquitylates subunits of the nascent polypeptide-associated complex (NAC) YER068C-A 0 295299 295730 OFF 5 YER068C-A complement(295299..295730) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER069W 1 295408 297999 ON 5 ARG5,6 (295408..297999) Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p YER070W 1 298948 301614 ON 5 RNR1 (298948..301614) Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits YER071C 0 301945 302325 ON 5 YER071C complement(301945..302325) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YER072W 1 302804 303193 ON 5 VTC1 (302804..303193) Vacuolar transporter chaperon (VTC) involved in distributing V-ATPase and other membrane proteins; together with other VTC proteins, forms a heterotetrameric complex that associates with the SNARE Nyv1p and the V0 sector of the V-ATPase YER073W 1 304027 305589 ON 5 ALD5 (304027..305589) Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed YER074W 1 306319 307192 ON 5 RPS24A (306319..306321,306788..307192) Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein YER074W-A 1 307649 308119 ON 5 YOS1 (307649..307742,307845..307952,308064..308119) Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p YER075C 0 308409 311195 ON 5 PTP3 complement(308409..311195) Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm YER076C 0 312586 313494 ON 5 YER076C complement(312586..313494) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization YER076W-A 1 313386 313733 OFF 5 YER076W-A (313386..313733) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER076C YER077C 0 314530 316596 ON 5 YER077C complement(314530..316596) Hypothetical protein YER078C 0 316803 318338 ON 5 YER078C complement(316803..318338) Metallopeptidase, localized to the mitochondrial matrix YER078W-A 1 318642 318806 OFF 5 YER078W-A (318642..318806) Putative protein of unknown function; identified by fungal homology and RT-PCR YER079W 1 318916 319548 ON 5 YER079W (318916..319548) Putative protein of unknown function YER079C-A 0 319898 320236 OFF 5 YER079C-A complement(319898..320236) Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER080W YER080W 1 319959 321842 ON 5 YER080W (319959..321842) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YER081W 1 322682 324091 ON 5 SER3 (322682..324091) 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser33p YER082C 0 324268 325932 ON 5 UTP7 complement(324268..325932) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YER083C 0 326170 327027 ON 5 GET2 complement(326170..327027) Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function YER084W 1 327061 327447 OFF 5 YER084W (327061..327447) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YER084W-A 1 327594 328106 OFF 5 YER084W-A (327594..328106) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER085C 0 327615 328136 ON 5 YER085C complement(327615..328136) Putative protein of unknown function YER086W 1 328473 330203 ON 5 ILV1 (328473..330203) Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation YER087W 1 330572 332302 ON 5 YER087W (330572..332302) Protein with similarity to tRNA synthetases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YER087C-A 0 331813 332364 OFF 5 YER087C-A complement(331813..332364) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase YER087C-B 0 332578 332826 ON 5 SBH1 complement(332578..332826) Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex; homologous to Sbh2p YER088C 0 333172 335184 ON 5 DOT6 complement(333172..335184) Protein of unknown function, involved in telomeric gene silencing and filamentation YER088C-A 0 335692 336015 OFF 5 YER088C-A complement(335692..336015) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER088W-B 1 335923 336069 OFF 5 YER088W-B (335923..336069) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER089C 0 335942 337336 ON 5 PTC2 complement(335942..337336) Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation YER090W 1 337945 339468 ON 5 TRP2 (337945..339468) Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p YER090C-A 0 338318 338407 OFF 5 YER090C-A complement(338318..338407) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER091C 0 339860 342163 ON 5 MET6 complement(339860..342163) Cobalamin-independent methionine synthase, involved in amino acid biosynthesis; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs YER091C-A 0 342386 342607 OFF 5 YER091C-A complement(342386..342607) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YER092W 1 342851 343228 ON 5 IES5 (342851..343228) Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions YER093C 0 343316 347608 ON 5 TSC11 complement(343316..347608) Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain YER093C-A 0 347908 348396 ON 5 YER093C-A complement(347908..348197,348273..348396) Putative protein of unknown function; YER093C-A contains an intron YER094C 0 348725 349342 ON 5 PUP3 complement(348725..349342) Beta subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 YER095W 1 349976 351178 ON 5 RAD51 (349976..351178) Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein YER096W 1 351694 353232 ON 5 SHC1 (351694..353232) Sporulation-specific activator of Chs3p (chitin synthase III), required for the synthesis of the chitosan layer of ascospores; has similarity to Skt5p, which activates Chs3p during vegetative growth; transcriptionally induced at alkaline pH YER097W 1 355136 355465 OFF 5 YER097W (355136..355465) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER098W 1 355462 357726 ON 5 UBP9 (355462..357726) Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease that cleaves ubiquitin-protein fusions YER099C 0 358101 359057 ON 5 PRS2 complement(358101..359057) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YER100W 1 359558 360310 ON 5 UBC6 (359558..360310) Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway YER101C 0 360498 361790 ON 5 AST2 complement(360498..361790) Protein that may have a role in targeting of plasma membrane [H+]ATPase (Pma1p) to the plasma membrane, as suggested by analysis of genetic interactions YER102W 1 363096 363698 ON 5 RPS8B (363096..363698) Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein YER103W 1 364585 366513 ON 5 SSA4 (364585..366513) Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation YER104W 1 366798 367424 ON 5 RTT105 (366798..367424) Protein with a role in regulation of Ty1 transposition YER105C 0 367834 372009 ON 5 NUP157 complement(367834..372009) Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p YER106W 1 372322 373230 ON 5 MAM1 (372322..373230) Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle YER107C 0 373444 374541 ON 5 GLE2 complement(373444..374541) Component of the nuclear pore complex required for polyadenylated RNA export but not for protein import, homologous to S. pombe Rae1p YER107W-A 1 374390 374710 OFF 5 YER107W-A (374390..374710) Dubious open reading frame unlikely to encode a protein, partially overlaps verified ORF GLE2/YER107C YER109C 0 375211 377610 ON 5 FLO8 complement(375211..377610) Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene YER110C 0 378758 382099 ON 5 KAP123 complement(378758..382099) Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 YER111C 0 382591 385872 ON 5 SWI4 complement(382591..385872) DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair YER112W 1 387228 387791 ON 5 LSM4 (387228..387791) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YER113C 0 387928 390048 ON 5 YER113C complement(387928..390048) Non-essential golgi localized protein of unknown function; contains niine potential transmembrane domains and has similarity to the endosomal protein, Emp70p YER114C 0 390586 393708 ON 5 BOI2 complement(390586..393708) Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain YER115C 0 394288 394863 ON 5 SPR6 complement(394288..394863) Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation YER116C 0 395344 396168 ON 5 SLX8 complement(395344..396168) Protein containing a RING finger domain that interacts with Hex3p; mutant phenotypes and genetic interactions suggest a role in sumoylation and in genome stability YER117W 1 396765 397649 ON 5 RPL23B (396765..396806,397278..397649) Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E. coli L14 and rat L23 ribosomal proteins YER118C 0 397948 399051 ON 5 SHO1 complement(397948..399051) Transmembrane osmosensor, participates in activation of both the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway YER119C 0 399492 400838 ON 5 AVT6 complement(399492..400838) Vacuolar amino acid transporter, exports aspartate and glutamate from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YER119C-A 0 400863 401234 OFF 5 YER119C-A complement(400863..401234) Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 YER120W 1 401131 401865 ON 5 SCS2 (401131..401865) Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog YER121W 1 402371 402715 OFF 5 YER121W (402371..402715) Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy YER122C 0 402867 404348 ON 5 GLO3 complement(402867..404348) ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p YER123W 1 404809 406383 ON 5 YCK3 (404809..406383) Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of the HOPS complex subunit, Vps41p; shares overlapping essential functions with Hrr25p YER124C 0 407338 409059 ON 5 DSE1 complement(407338..409059) Daughter cell-specific protein, may participate in pathways regulating cell wall metabolism; deletion affects cell separation after division and sensitivity to drugs targeted against the cell wall YER125W 1 410185 412614 ON 5 RSP5 (410185..412614) Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation; functions in multivesicular body sorting, heat shock response and ubiquitylation of arrested RNAPII; contains a hect (homologous to E6-AP carboxyl terminus) domain YER126C 0 413390 414175 ON 5 NSA2 complement(413390..414175) Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA YER127W 1 414477 415550 ON 5 LCP5 (414477..415550) Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus YER128W 1 415855 416466 ON 5 YER128W (415855..416466) Putative protein of unknown function YER129W 1 417277 420705 ON 5 SAK1 (417277..420705) Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome YER130C 0 421111 422442 ON 5 YER130C complement(421111..422442) Hypothetical protein YER131W 1 423948 424307 ON 5 RPS26B (423948..424307) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similarity to rat S26 ribosomal protein YER132C 0 425184 430445 ON 5 PMD1 complement(425184..430445) Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions YER133W 1 432491 433954 ON 5 GLC7 (432491..432667,433193..433954) Catalytic subunit of type 1 serine/threonine protein phosphatase, involved in many processes including glycogen metabolism, sporulation, and mitosis; interacts with multiple regulatory subunits; predominantly isolated with Sds22p YER133W-A 1 437187 437528 OFF 5 YER133W-A (437187..437528) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YER134C. YER134C 0 437263 437799 ON 5 YER134C complement(437263..437799) Putative protein of unknown function; non-essential gene YER135C 0 437948 438340 OFF 5 YER135C complement(437948..438340) Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene YER136W 1 439612 440967 ON 5 GDI1 (439612..440967) GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins YER137C 0 441369 441815 ON 5 YER137C complement(441369..441815) Putative protein of unknown function YER137W-A 1 441571 441876 OFF 5 YER137W-A (441571..441876) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER138W-A 1 449470 449574 OFF 5 YER138W-A (449470..449574) Putative protein of unknown function YER139C 0 450558 451238 ON 5 YER139C complement(450558..451238) Putative protein of unknown function; YER139C has been shown to be required for growth at high temperatures; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YER140W 1 451560 453230 ON 5 YER140W (451560..453230) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YER141W 1 453454 454914 ON 5 COX15 (453454..454914) Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase YER142C 0 455141 456031 ON 5 MAG1 complement(455141..456031) 3-methyl-adenine DNA glycosylase involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired YER143W 1 456314 457600 ON 5 DDI1 (456314..457600) DNA damage-inducible v-SNARE binding protein, contains a ubiquitin-associated (UBA) domain, may act as a negative regulator of constitutive exocytosis, may play a role in S-phase checkpoint control YER144C 0 457801 460218 ON 5 UBP5 complement(457801..460218) Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck YER145C 0 460521 461735 ON 5 FTR1 complement(460521..461735) High affinity iron permease involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron YER145C-A 0 462381 462818 OFF 5 YER145C-A complement(462381..462818) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF LSM5/YER146W YER146W 1 462580 462861 ON 5 LSM5 (462580..462861) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YER147C 0 462963 464837 ON 5 SCC4 complement(462963..464837) Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX YER147C-A 0 465198 465608 OFF 5 YER147C-A complement(465198..465608) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER148W 1 465298 466020 ON 5 SPT15 (465298..466020) TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability YER148W-A 1 466180 466758 OFF 5 YER148W-A (466180..466758) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER149C 0 466203 467465 ON 5 PEA2 complement(466203..467465) Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth YER150W 1 468365 468811 ON 5 SPI1 (468365..468811) GPI-anchored, serine/threonine rich cell wall protein of unknown function; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p YER151C 0 469681 472419 ON 5 UBP3 complement(469681..472419) Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi compartments; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin YER152C 0 472652 473983 ON 5 YER152C complement(472652..473983) Putative protein of unknown function; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene YER152W-A 1 473480 474046 OFF 5 YER152W-A (473480..474046) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YER153C 0 474036 474800 ON 5 PET122 complement(474036..474800) Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane YER154W 1 475015 476223 ON 5 OXA1 (475015..476223) Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals YER155C 0 476340 482843 ON 5 BEM2 complement(476340..482843) Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence YER156C 0 483320 484336 ON 5 YER156C complement(483320..484336) Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YER157W 1 484783 487188 ON 5 COG3 (484783..487188) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YER158C 0 488852 490573 ON 5 YER158C complement(488852..490573) Protein of unknown function, has similarity to Afr1p; potentially phosphorylated by Cdc28p YER158W-A 1 491482 491697 OFF 5 YER158W-A (491482..491697) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER159C 0 491525 491953 ON 5 BUR6 complement(491525..491953) Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha YER161C 0 499342 500343 ON 5 SPT2 complement(499342..500343) Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins YER162C 0 500625 502889 ON 5 RAD4 complement(500625..502889) Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human XPC protein YER163C 0 503079 503777 ON 5 YER163C complement(503079..503777) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YER164W 1 505387 509793 ON 5 CHD1 (505387..509793) Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes YER165W 1 510368 512101 ON 5 PAB1 (510368..512101) Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G YER165C-A 0 512622 512978 OFF 5 YER165C-A complement(512622..512978) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DNF1/YER166W YER166W 1 512739 517454 ON 5 DNF1 (512739..517454) Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase YER167W 1 518211 520766 ON 5 BCK2 (518211..520766) Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations YER168C 0 521024 522664 ON 5 CCA1 complement(521024..522664) ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites YER169W 1 523364 525754 ON 5 RPH1 (523364..525754) JmjC domain-containing histone demethylase which can specifically demethylate H3K36 tri- and dimethyl modification states; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway YER170W 1 525969 526646 ON 5 ADK2 (525969..526646) Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background YER171W 1 527077 529413 ON 5 RAD3 (527077..529413) 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA polymerase II transcription initiation factor TFIIH; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein YER172C 0 529525 536016 ON 5 BRR2 complement(529525..536016) RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD YER172C-A 0 536270 536650 OFF 5 YER172C-A complement(536270..536650) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YER173W 1 536295 538274 ON 5 RAD24 (536295..538274) Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein YER174C 0 538429 539163 ON 5 GRX4 complement(538429..539163) Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage YER175C 0 539459 540358 ON 5 TMT1 complement(539459..540358) Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle YER175W-A 1 540645 540809 OFF 5 YER175W-A (540645..540809) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER176W 1 541685 545050 ON 5 ECM32 (541685..545050) DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes YER177W 1 545606 546409 ON 5 BMH1 (545606..546409) 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling YER178W 1 546812 548074 ON 5 PDA1 (546812..548074) E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA, regulated by glucose YER179W 1 548416 549512 ON 5 DMC1 (548416..548547,548640..549512) Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein YER180C 0 549719 550522 ON 5 ISC10 complement(549719..550522) Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells YER180C-A 0 550860 551117 ON 5 SLO1 complement(550860..551117) Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO YER181C 0 551468 551791 OFF 5 YER181C complement(551468..551791) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies YER182W 1 552520 553254 ON 5 YER182W (552520..553254) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YER183C 0 553329 553964 ON 5 FAU1 complement(553329..553964) 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis YER184C 0 556291 558675 ON 5 YER184C complement(556291..558675) Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source YER185W 1 559449 560360 ON 5 YER185W (559449..560360) Putative protein of unknown function; topology models predict that YER185W may encode an integral membrane protein; similar in sequence to RSB1, which is involved in sphingoid long-chain base release YER186C 0 561700 562620 ON 5 YER186C complement(561700..562620) Putative protein of unknown function YER187W 1 566225 566650 ON 5 YER187W (566225..566650) Putative protein of unknown function; induced in respiratory-deficient cells YER188W 1 568035 568754 OFF 5 YER188W (568035..568754) Hypothetical protein YER188C-A 0 569603 569902 OFF 5 YER188C-A complement(569603..569902) Putative protein of unknown function YER189W 1 571150 571518 ON 5 YER189W (571150..571518) Putative protein of unknown function YER190W 1 571475 576520 ON 5 YRF1-2 (571475..576520) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YER190C-A 0 574799 575374 OFF 5 YER190C-A complement(574799..575374) Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YER190C-B 0 575675 576157 OFF 5 YER190C-B complement(575675..576157) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFL068W 1 53 535 ON 6 YFL068W (53..535) Putative protein of unknown function YFL067W 1 836 1363 OFF 6 YFL067W (836..1363) Protein of unknown function, down-regulated at low calcium levels YFL066C 0 1437 2615 ON 6 YFL066C complement(1437..2615) Helicase-like protein encoded within the telomeric Y' element YFL065C 0 3030 3338 ON 6 YFL065C complement(3030..3338) Putative protein of unknown function YFL064C 0 3322 3846 ON 6 YFL064C complement(3322..3846) Putative protein of unknown function YFL063W 1 5066 5521 OFF 6 YFL063W (5066..5521) Dubious open reading frame, based on available experimental and comparative sequence data YFL062W 1 6426 7565 ON 6 COS4 (6426..7565) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YFL061W 1 9545 10222 ON 6 YFL061W (9545..10222) Protein whose expression is induced by DNA damage YFL060C 0 10301 10969 ON 6 SNO3 complement(10301..10969) Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin YFL059W 1 11363 12259 ON 6 SNZ3 (11363..12259) Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3 YFL058W 1 12929 13951 ON 6 THI5 (12929..13951) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YFL057C 0 14305 14763 ON 6 AAD16 complement(14305..14763) Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YFL056C 0 14793 15431 ON 6 AAD6 complement(14793..15431) Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase, involved in the oxidative stress response YFL055W 1 17004 18680 ON 6 AGP3 (17004..18680) Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation YFL054C 0 20847 22787 ON 6 YFL054C complement(20847..22787) Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol YFL053W 1 23423 25198 ON 6 DAK2 (23423..25198) Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation YFL052W 1 28232 29629 ON 6 YFL052W (28232..29629) Putative zinc cluster protein that contains a DNA binding domain; null mutant sensitive to calcofluor white, low osmolarity and heat, suggesting a role for YFL052Wp in cell wall integrity YFL051C 0 30058 30540 ON 6 YFL051C complement(30058..30540) Putative protein of unknown function; YFL051C is not an essential gene YFL050C 0 33272 35848 ON 6 ALR2 complement(33272..35848) Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions YFL049W 1 36803 38674 ON 6 SWP82 (36803..38674) Member of the SWI/SNF chromatin remodeling complex in which it plays an as yet unidentified role; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p YFL048C 0 38843 40180 ON 6 EMP47 complement(38843..40180) Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport YFL047W 1 40421 42565 ON 6 RGD2 (40421..42565) GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p YFL046W 1 42815 43438 ON 6 YFL046W (42815..43438) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YFL045C 0 43628 44392 ON 6 SEC53 complement(43628..44392) Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen YFL044C 0 44655 45560 ON 6 OTU1 complement(44655..45560) Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family YFL042C 0 45720 47744 ON 6 YFL042C complement(45720..47744) Putative protein of unknown function; YFL042C is not an essential gene YFL041W-A 1 48734 48925 OFF 6 YFL041W-A (48734..48925) Putative protein of unknown function; identified by fungal homology and RT-PCR YFL041W 1 49139 51007 ON 6 FET5 (49139..51007) Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport YFL040W 1 51350 52972 ON 6 YFL040W (51350..52972) Putative transporter, member of the sugar porter family; YFL040W is not an essential gene YFL039C 0 53260 54695 ON 6 ACT1 complement(53260..54377,54686..54695) Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions YFL038C 0 55365 55985 ON 6 YPT1 complement(55365..55985) Ras-like small GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) YFL037W 1 56335 57708 ON 6 TUB2 (56335..57708) Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules YFL036W 1 58781 62836 ON 6 RPO41 (58781..62836) Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition YFL034C-B 0 63015 63992 ON 6 MOB2 complement(63015..63858,63973..63992) Component of the RAM signaling network, localizes and activates the Ace2p in the daughter cell nucleus to direct daughter cell-specific transcription of several genes involved in cell separation; Mob1p-like protein YFL034C-A 0 64242 64931 ON 6 RPL22B complement(64242..64598,64920..64931) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Ap and to rat L22 ribosomal protein YFL034W 1 65475 68696 ON 6 YFL034W (65475..68696) Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk YFL033C 0 69113 74425 ON 6 RIM15 complement(69113..74425) Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase YFL032W 1 74870 75190 OFF 6 YFL032W (74870..75190) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene YFL031W 1 75177 76145 ON 6 HAC1 (75177..75837,76090..76145) bZIP transcription factor (ATF/CREB1 homolog) that regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway utilizing Ire1p, Trl1p and Ada5p facilitates efficient Hac1p synthesis YFL031C-A 0 76012 76113 OFF 6 YFL031C-A complement(76012..76113) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps HAC1/YFL031W; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFL030W 1 76829 77986 ON 6 AGX1 (76829..77986) Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases YFL029C 0 78053 79159 ON 6 CAK1 complement(78053..79159) Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases YFL028C 0 79342 80211 ON 6 CAF16 complement(79342..80211) Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation; putative ABC ATPase; interacts with Ssn2p, Ssn3p, and Ssn8p YFL027C 0 80417 81910 ON 6 GYP8 complement(80417..81910) GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport YFL026W 1 82578 83873 ON 6 STE2 (82578..83873) Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells YFL025C 0 84143 87232 ON 6 BST1 complement(84143..87232) GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules YFL024C 0 87845 90343 ON 6 EPL1 complement(87845..90343) Component of NuA4, which is an essential histone H4/H2A acetyltransferase complex; homologous to Drosophila Enhancer of Polycomb YFL023W 1 90984 93374 ON 6 BUD27 (90984..93374) Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YFL022C 0 93497 95008 ON 6 FRS2 complement(93497..95008) Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar YFL021C-A 0 95759 96613 OFF 6 YFL021C-A complement(95759..96613) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFL021W 1 95964 97496 ON 6 GAT1 (95964..97496) Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p YFL020C 0 99225 99593 ON 6 PAU5 complement(99225..99593) Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YFL019C 0 100246 100599 OFF 6 YFL019C complement(100246..100599) Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene YFL018C 0 101622 103121 ON 6 LPD1 complement(101622..103121) Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes YFL017W-A 1 103693 103926 ON 6 SMX2 (103693..103926) Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G YFL017C 0 103977 104456 ON 6 GNA1 complement(103977..104456) Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA YFL016C 0 104695 106230 ON 6 MDJ1 complement(104695..106230) Protein involved in folding of mitochondrially synthesized proteins in the mitochondrial matrix; localizes to the mitochondrial inner membrane; member of the DnaJ family of molecular chaperones YFL015W-A 1 106409 106726 OFF 6 YFL015W-A (106409..106726) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFL015C 0 106463 106957 OFF 6 YFL015C complement(106463..106957) Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene YFL014W 1 107250 107579 ON 6 HSP12 (107250..107579) Plasma membrane localized protein that protects membranes from desiccation; induced by heat shock, oxidative stress, osmostress, stationary phase entry, glucose depletion, oleate and alcohol; regulated by the HOG and Ras-Pka pathways YFL013W-A 1 107793 108596 OFF 6 YFL013W-A (107793..108596) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YFL013C 0 107846 109924 ON 6 IES1 complement(107846..109924) Subunit of the INO80 chromatin remodeling complex YFL012W-A 1 109804 110178 OFF 6 YFL012W-A (109804..110178) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene IES1/YFL013C YFL012W 1 110641 111087 ON 6 YFL012W (110641..111087) Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin YFL011W 1 112339 113979 ON 6 HXT10 (112339..113979) Putative hexose transporter, expressed at low levels and expression is repressed by glucose YFL010W-A 1 114984 115268 OFF 6 AUA1 (114984..115268) Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease YFL010C 0 115102 115737 ON 6 WWM1 complement(115102..115737) WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes Gi phase growth arrest and clonal death that is suppressed by overexpression of MCA1 YFL009W 1 116139 118478 ON 6 CDC4 (116139..118478) F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p YFL008W 1 119424 123101 ON 6 SMC1 (119424..123101) Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure YFL007W 1 123474 129905 ON 6 BLM10 (123474..129905) Proteosome activator subunit; found in association with core particles, with and without the 19S regulatory particle; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 YFL005W 1 130329 130976 ON 6 SEC4 (130329..130976) Secretory vesicle-associated Rab GTPase essential for exocytosis; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane YFL004W 1 131805 134291 ON 6 VTC2 (131805..134291) Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in protein localization and non-autophagic vacuolar fusion YFL003C 0 134516 137152 ON 6 MSH4 complement(134516..137152) Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein YFL002C 0 145109 146929 ON 6 SPB4 complement(145109..146929) Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients YFL001W 1 147126 148454 ON 6 DEG1 (147126..148454) Non-essential tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm YFR001W 1 149105 149719 ON 6 LOC1 (149105..149719) Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles YFR002W 1 150011 152530 ON 6 NIC96 (150011..152530) Component of the nuclear pore complex, required for nuclear pore formation; forms a subcomplex with Nsp1p, Nup57p, and Nup49p YFR003C 0 152652 153119 ON 6 YPI1 complement(152652..153119) Inhibitor of the type I protein phosphatase Glc7p, which is involved in regulation of a variety of metabolic processes; overproduction causes decreased cellular content of glycogen YFR004W 1 153388 154308 ON 6 RPN11 (153388..154308) Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates YFR005C 0 154522 155868 ON 6 SAD1 complement(154522..155868) Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle YFR006W 1 156139 157746 ON 6 YFR006W (156139..157746) Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene YFR007W 1 159293 160354 ON 6 YFR007W (159293..160354) Putative protein of unknown function with similarity to pantothenate kinases from other organisms; YFR007W is not an essential gene YFR008W 1 160529 161194 ON 6 FAR7 (160529..161194) Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p YFR009W 1 162482 164740 ON 6 GCN20 (162482..164740) Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA YFR009W-A 1 163869 164126 OFF 6 YFR009W-A (163869..164126) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YFR009W; identified by expression profiling and mass spectrometry YFR010W 1 165060 166559 ON 6 UBP6 (165060..166559) Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds YFR010W-A 1 166721 166909 OFF 6 YFR010W-A (166721..166909) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YFR011C; identified by expression profiling and mass spectrometry YFR011C 0 166739 167251 ON 6 YFR011C complement(166739..167251) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YFR011C is not an essential gene YFR012W 1 167881 168489 ON 6 YFR012W (167881..168489) Putative protein of unknown function YFR012W-A 1 169216 169302 OFF 6 YFR012W-A (169216..169302) Putative protein of unknown function; identified by homology YFR013W 1 169914 172277 ON 6 IOC3 (169914..172277) Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing YFR014C 0 172529 173869 ON 6 CMK1 complement(172529..173869) Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian Cam Kinase II YFR015C 0 174257 176383 ON 6 GSY1 complement(174257..176383) Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase YFR016C 0 177034 180735 ON 6 YFR016C complement(177034..180735) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene YFR017C 0 182262 182849 ON 6 YFR017C complement(182262..182849) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YFR017C is not an essential gene YFR018C 0 183119 184210 ON 6 YFR018C complement(183119..184210) Putative protein of unknown function YFR019W 1 184490 191326 ON 6 FAB1 (184490..191326) 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis YFR020W 1 192726 193424 OFF 6 YFR020W (192726..193424) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFR021W 1 194800 196302 ON 6 ATG18 (194800..196302) Phosphatidylinositol 3,5-bisphosphate-binding protein of the vacuolar membrane, predicted to fold as a seven-bladed beta-propeller; required for recycling of Atg9p through the pre-autophagosomal structure YFR022W 1 196821 199022 ON 6 ROG3 (196821..199022) Protein that binds to Rsp5p, which is a hect-type ubiquitin ligase, via its 2 PY motifs; has similarity to Rod1p; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant YFR023W 1 199862 201697 ON 6 PES4 (199862..201697) Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation, similar to Mip6p YFR024C-A 0 201948 203421 ON 6 LSB3 complement(201948..203256,203375..203421) Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YFR025C 0 203731 204738 ON 6 HIS2 complement(203731..204738) Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control YFR026C 0 205736 206245 ON 6 YFR026C complement(205736..206245) Putative protein of unknown function YFR027W 1 207440 208285 ON 6 ECO1 (207440..208285) Acetyltransferase required for the establishment of sister chromatid cohesion during DNA replication, but not for its maintenance during G2 and M phases; also required for postreplicative double-strand break repair; interacts with Chl1p YFR028C 0 208401 210056 ON 6 CDC14 complement(208401..210056) Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit YFR029W 1 210925 212961 ON 6 PTR3 (210925..212961) Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes YFR030W 1 213300 216407 ON 6 MET10 (213300..216407) Subunit alpha of assimilatory sulfite reductase, which is responsible for the conversion of sulfite into sulfide YFR031C 0 216582 220094 ON 6 SMC2 complement(216582..220094) Component of the condensin complex, essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA, possibly in the cleft formed by the coiled-coil of the folded dimer YFR031C-A 0 220495 221406 ON 6 RPL2A complement(220495..221255,221403..221406) Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins YFR032C 0 222078 222947 ON 6 YFR032C complement(222078..222947) Putative protein of unknown function; transcribed during sporulation; YFR032C is not an essential gene YFR032C-A 0 223246 223425 ON 6 RPL29 complement(223246..223425) Protein component of the large (60S) ribosomal subunit, has similarity to rat L29 ribosomal protein; not essential for translation, but required for proper joining of the large and small ribosomal subunits and for normal translation rate YFR032C-B 0 223698 223961 OFF 6 YFR032C-B complement(223698..223961) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YFR033C 0 224314 224757 ON 6 QCR6 complement(224314..224757) Subunit 6 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1 YFR034C 0 225008 225946 ON 6 PHO4 complement(225008..225946) Basic helix-loop-helix (bHLH) transcription factor of the myc-family; binds cooperatively with Pho2p to the PHO5 promoter; function is regulated by phosphorylation at multiple sites and by phosphate availability YFR034W-A 1 226010 226297 OFF 6 YFR034W-A (226010..226297) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YFR035C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching YFR035C 0 226109 226453 OFF 6 YFR035C complement(226109..226453) Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Scer_YGOB_ADL119W 1 226248 226538 ON 6 ADL119W (226248..226538) ANNOTATED BY YGOB - YFR036W 1 226950 227324 ON 6 CDC26 (226950..227324) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YFR036W-A 1 227438 228088 OFF 6 YFR036W-A (227438..228088) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene RSC8 YFR037C 0 227500 229173 ON 6 RSC8 complement(227500..229173) Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters YFR038W 1 229367 231928 ON 6 YFR038W (229367..231928) Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52 foci YFR039C 0 231999 233531 ON 6 YFR039C complement(231999..233531) Putative protein of unknown function; YFR039C is not an essential gene YFR040W 1 234229 237237 ON 6 SAP155 (234229..237237) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p YFR041C 0 237355 238242 ON 6 ERJ5 complement(237355..238242) Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response YFR042W 1 238445 239047 ON 6 YFR042W (238445..239047) Protein of unknown function required for cell viability; localizes to the endoplasmic reticulum membrane YFR043C 0 239101 239814 ON 6 YFR043C complement(239101..239814) Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci YFR044C 0 239979 241424 ON 6 DUG1 complement(239979..241424) Probable di- and tri-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) YFR045W 1 241985 242986 ON 6 YFR045W (241985..241996,242069..242986) Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white YFR046C 0 243061 244146 ON 6 CNN1 complement(243061..244146) Kinetochore protein of unknown function; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by both Clb5-Cdk1 and, to a lesser extent, Clb2-Cdk1. YFR047C 0 244266 245153 ON 6 BNA6 complement(244266..245153) Quinolinate phosphoribosyl transferase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway YFR048W 1 246133 248121 ON 6 RMD8 (246133..248121) Cytosolic protein required for sporulation YFR049W 1 248510 248881 ON 6 YMR31 (248510..248881) Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 YFR050C 0 249053 249853 ON 6 PRE4 complement(249053..249853) 20S proteasome beta-type subunit YFR051C 0 250150 251790 ON 6 RET2 complement(250150..251790) Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER YFR052W 1 252492 253316 ON 6 RPN12 (252492..253316) Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p YFR052C-A 0 253416 253721 OFF 6 YFR052C-A complement(253416..253721) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFR053C 0 253579 255036 ON 6 HXK1 complement(253579..255036) Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves the hexokinase Hxk2p YFR054C 0 258842 259420 OFF 6 YFR054C complement(258842..259420) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YFR056C 0 263944 264312 OFF 6 YFR056C complement(263944..264312) Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W YFR055W 1 264191 265213 ON 6 YFR055W (264191..265213) Putative cystathionine beta-lyase; involved in copper ion homeostasis and sulfur metabolism; null mutant displays increased levels of spontaneous Rad52 foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner YFR057W 1 269048 269503 OFF 6 YFR057W (269048..269503) Putative protein of unknown function YGL263W 1 2790 3932 ON 7 COS12 (2790..3932) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YGL262W 1 5312 5839 ON 7 YGL262W (5312..5839) Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene YGL261C 0 6290 6652 ON 7 YGL261C complement(6290..6652) Putative protein of unknown function; mRNA expression appears to be regulated by SUT1 and UPC2 YGL260W 1 6860 7090 OFF 7 YGL260W (6860..7090) Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium YGL259W 1 8470 8967 ON 7 YPS5 (8470..8967) Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p YGL258W-A 1 9162 9395 ON 7 YGL258W-A (9162..9395) Putative protein of unknown function YGL258W 1 11110 11730 ON 7 VEL1 (11110..11730) Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants YGL257C 0 12481 14157 ON 7 MNT2 complement(12481..14157) Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans YGL256W 1 14910 16307 ON 7 ADH4 (14910..16307) Alcohol dehydrogenase type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency; alcohol dehydrogenase isoenzyme IV YGL255W 1 20978 22108 ON 7 ZRT1 (20978..22108) High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor YGL254W 1 22304 23203 ON 7 FZF1 (22304..23203) Transcription factor involved in sulfite metabolism, sole identified regulatory target is SSU1, overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers YGL253W 1 23935 25395 ON 7 HXK2 (23935..25395) Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene YGL252C 0 25718 27484 ON 7 RTG2 complement(25718..27484) Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p YGL251C 0 27921 31636 ON 7 HFM1 complement(27921..31426,31579..31636) Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity YGL250W 1 31910 32635 ON 7 YGL250W (31910..32635) Putative protein of unknown function; deletion mutant results in reduced PIS1 expression and has growth defects on non-fermentable carbon sources and on minimal media; GFP-fusion protein localizes to both the cytoplasm and the nucleus YGL249W 1 33098 35212 ON 7 ZIP2 (33098..35212) Meiosis-specific protein involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis YGL248W 1 35653 36762 ON 7 PDE1 (35653..36762) Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation YGL247W 1 36933 37526 ON 7 BRR6 (36933..37526) Essential nuclear envelope integral membrane protein required for nuclear transport; depletion alters nucleoporin distribution and nuclear envelope morphology, suggesting a role in the spatial organization of nuclear pores YGL246C 0 37617 38780 ON 7 RAI1 complement(37617..38780) Nuclear protein that binds to and stabilizes the exoribonuclease Rat1p, required for pre-rRNA processing YGL245W 1 39023 41149 ON 7 GUS1 (39023..41149) Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm YGL244W 1 41498 43174 ON 7 RTF1 (41498..43174) Subunit of the RNA polymerase II-associated Paf1 complex; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in telomere maintenance YGL243W 1 43307 44509 ON 7 TAD1 (43307..44509) tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala YGL242C 0 44652 45197 ON 7 YGL242C complement(44652..45197) Putative protein of unknown function; deletion mutant is viable YGL241W 1 45445 48459 ON 7 KAP114 (45445..48459) Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear YGL240W 1 48613 49365 ON 7 DOC1 (48613..49365) Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis YGL239C 0 49431 49745 OFF 7 YGL239C complement(49431..49745) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene CSE1 YGL238W 1 49552 52434 ON 7 CSE1 (49552..52434) Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation YGL237C 0 52731 53528 ON 7 HAP2 complement(52731..53528) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding YGL236C 0 53787 55796 ON 7 MTO1 complement(53787..55796) Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants YGL235W 1 55279 55815 OFF 7 YGL235W (55279..55815) Putative protein of unknown function; potential Cdc28p substrate YGL234W 1 56482 58890 ON 7 ADE5,7 (56482..58890) Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities YGL233W 1 59122 61854 ON 7 SEC15 (59122..61854) Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which mediates polarized targeting of vesicles to active sites of exocytosis; Sec15p associates with Sec4p and vesicles YGL232W 1 62075 63002 ON 7 TAN1 (62075..62131,62190..63002) Putative tRNA acetyltransferase, RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA YGL231C 0 63048 63620 ON 7 YGL231C complement(63048..63620) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YGL230C 0 63772 64215 ON 7 YGL230C complement(63772..64215) Putative protein of unknown function; non-essential gene YGL229C 0 64503 66959 ON 7 SAP4 complement(64503..66959) Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p YGL228W 1 67598 69331 ON 7 SHE10 (67598..69331) Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest YGL227W 1 69671 72547 ON 7 VID30 (69671..72547) Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); shifts the balance of nitrogen metabolism toward the production of glutamate; localized to the nucleus and the cytoplasm YGL226C-A 0 72748 73157 ON 7 OST5 complement(72748..72987,73137..73157) Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins YGL226W 1 73339 73710 ON 7 YGL226W (73339..73710) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YGL225W 1 76893 77906 ON 7 VRG4 (76893..77906) Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi YGL224C 0 78014 78856 ON 7 SDT1 complement(78014..78856) Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives YGL223C 0 79111 80364 ON 7 COG1 complement(79111..80364) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YGL222C 0 80658 81185 ON 7 EDC1 complement(80658..81185) RNA-binding protein, activates mRNA decapping directly by binding to the mRNA substrate and enhancing the activity of the decapping proteins Dcp1p and Dcp2p YGL221C 0 81425 82291 ON 7 NIF3 complement(81425..82291) Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL220W 1 82373 82735 ON 7 YGL220W (82373..82735) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus YGL219C 0 82877 84256 ON 7 MDM34 complement(82877..84256) Mitochondrial outer membrane protein, required for normal mitochondrial morphology and inheritance; localizes to dots on the mitochondrial surface near mtDNA nucleoids; interacts genetically with MDM31 and MDM32 YGL218W 1 83649 84299 OFF 7 YGL218W (83649..84299) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance YGL217C 0 84838 85179 OFF 7 YGL217C complement(84838..85179) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W YGL216W 1 84884 87301 ON 7 KIP3 (84884..87301) Kinesin-related motor protein involved in mitotic spindle positioning YGL215W 1 87980 89338 ON 7 CLG1 (87980..89338) Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 YGL214W 1 90011 90499 OFF 7 YGL214W (90011..90499) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C YGL213C 0 90058 91251 ON 7 SKI8 complement(90058..91251) Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs as well as double-strand break formation during meiotic recombination; required for repressing propagation of dsRNA viruses YGL212W 1 91436 92386 ON 7 VAM7 (91436..92386) Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; functions with a syntaxin homolog Vam3p in vacuolar protein trafficking YGL211W 1 92516 93595 ON 7 NCS6 (92516..93595) Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae YGL210W 1 93797 94465 ON 7 YPT32 (93797..94465) GTPase of the Ypt/Rab family, very similar to Ypt31p; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi YGL209W 1 95862 97010 ON 7 MIG2 (95862..97010) Protein containing zinc fingers, involved in repression, along with Mig1p, of SUC2 (invertase) expression by high levels of glucose; binds to Mig1p-binding sites in SUC2 promoter YGL208W 1 97342 98589 ON 7 SIP2 (97342..98589) One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane YGL207W 1 98973 102080 ON 7 SPT16 (98973..102080) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), facilitates RNA Polymerase II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure YGL206C 0 102547 107508 ON 7 CHC1 complement(102547..107508) Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function YGL205W 1 108162 110408 ON 7 POX1 (108162..110408) Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix YGL204C 0 111704 112009 OFF 7 YGL204C complement(111704..112009) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL203C 0 112479 114668 ON 7 KEX1 complement(112479..114668) Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins YGL202W 1 116063 117565 ON 7 ARO8 (116063..117565) Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis YGL201C 0 117858 120911 ON 7 MCM6 complement(117858..120911) Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex YGL200C 0 122699 123310 ON 7 EMP24 complement(122699..123310) Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport YGL199C 0 123577 124047 OFF 7 YGL199C complement(123577..124047) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W YGL198W 1 123596 124303 ON 7 YIP4 (123596..124303) Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport YGL197W 1 124703 129166 ON 7 MDS3 (124703..129166) Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions YGL196W 1 129888 131174 ON 7 YGL196W (129888..131174) Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype YGL195W 1 131531 139549 ON 7 GCN1 (131531..139549) Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA YGL194C-A 0 139725 139967 ON 7 YGL194C-A complement(139725..139967) Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species YGL194C 0 140374 141732 ON 7 HOS2 complement(140374..141732) Histone deacetylase required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity YGL193C 0 141922 142233 OFF 7 YGL193C complement(141922..142233) Haploid-specific gene repressed by a1-alpha2, turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold YGL192W 1 142252 144054 ON 7 IME4 (142252..144054) Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells YGL191W 1 144814 145203 ON 7 COX13 (144814..145203) Subunit VIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP YGL190C 0 145815 147395 ON 7 CDC55 complement(145815..147395) Non-essential regulatory subunit B of protein phosphatase 2A; has multiple roles in mitosis and protein biosynthesis; found in the nucleus of most cells but also at the bud neck (large-budded cells) and at the bud tip (small-budded cells) YGL189C 0 148235 148594 ON 7 RPS26A complement(148235..148594) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similarity to rat S26 ribosomal protein YGL188C-A 0 148830 148970 OFF 7 YGL188C-A complement(148830..148970) Putative protein of unknown function YGL188C 0 149349 149522 OFF 7 YGL188C complement(149349..149522) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL187C 0 149710 150177 ON 7 COX4 complement(149710..150177) Subunit IV of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; N-terminal 25 residues of precursor are cleaved during mitochondrial import YGL186C 0 151043 152782 ON 7 TPN1 complement(151043..152782) Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p YGL185C 0 153173 154312 ON 7 YGL185C complement(153173..154312) Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGL184C 0 154621 156018 ON 7 STR3 complement(154621..156018) Cystathionine beta-lyase, converts cystathionine into homocysteine YGL183C 0 156549 157291 ON 7 MND1 complement(156549..157205,157289..157291) Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks YGL182C 0 157279 157602 OFF 7 YGL182C complement(157279..157602) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MND1/YGL183C YGL181W 1 157912 159102 ON 7 GTS1 (157912..159102) Protein containing a zinc-finger in the N-terminus and a long Gln-rich region in the C-terminus; regulates ultradian rhythm, cell size, cell cycle, lifespan, sporulation, heat tolerance, and multidrug transport YGL180W 1 160071 162764 ON 7 ATG1 (160071..162764) Protein serine/threonine kinase, required for autophagy and for the cytoplasm-to-vacuole targeting (Cvt) pathway YGL179C 0 163415 165097 ON 7 TOS3 complement(163415..165097) Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome YGL178W 1 167358 170577 ON 7 MPT5 (167358..167360,168001..170577) Member of the Puf family of RNA-binding proteins; binds to mRNAs encoding chromatin modifiers and spindle pole body components; involved in longevity, maintenance of cell wall integrity, and sensitivity to and recovery from pheromone arrest YGL177W 1 167565 167912 OFF 7 YGL177W (167565..167912) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL176C 0 171421 173085 ON 7 YGL176C complement(171421..173085) Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype YGL175C 0 173291 174328 ON 7 SAE2 complement(173291..174328) Protein with a role in accurate meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents YGL174W 1 174551 175351 ON 7 BUD13 (174551..175351) Subunit of the RES complex, which is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern YGL173C 0 175533 180119 ON 7 KEM1 complement(175533..180119) Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved in mRNA decay; plays a role in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, and telomere maintenance YGL172W 1 180706 182124 ON 7 NUP49 (180706..182124) Subunit of the Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of the nuclear pore complex (NPC), required for nuclear export of ribosomes YGL171W 1 182396 184090 ON 7 ROK1 (182396..184090) ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis YGL170C 0 184159 185400 ON 7 SPO74 complement(184159..185400) Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation YGL169W 1 186065 187345 ON 7 SUA5 (186065..187345) Protein required for respiratory growth; null mutation suppresses the Cyc1p translation defect caused by the presence of an aberrant ATG codon upstream of the correct start YGL168W 1 187470 187802 OFF 7 HUR1 (187470..187802) Protein required for hydroxyurea resistance; has possible roles in DNA replication and maintenance of proper telomere length YGL167C 0 187622 190474 ON 7 PMR1 complement(187622..190474) High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease YGL166W 1 191135 191812 ON 7 CUP2 (191135..191812) Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations YGL165C 0 191404 191982 OFF 7 YGL165C complement(191404..191982) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W YGL164C 0 191984 193306 ON 7 YRB30 complement(191984..193306) RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes YGL163C 0 193713 196409 ON 7 RAD54 complement(193713..196409) DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family YGL162W 1 198144 199043 ON 7 SUT1 (198144..199043) Transcription factor of the Zn[II]2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression YGL161C 0 199216 200148 ON 7 YIP5 complement(199216..200148) Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport YGL160W 1 200567 202279 ON 7 YGL160W (200567..202279) Putative protein of uknown function with sequence similarity to iron/copper reductases (FRE1-8), possibly involved in iron homeostasis; may interact with ribosomes, based on co-purification experiments YGL159W 1 202727 203839 ON 7 YGL159W (202727..203839) Putative protein of unknown function; deletion mutant has no detectable phenotype YGL158W 1 207038 208576 ON 7 RCK1 (207038..208576) Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations YGL157W 1 209011 210054 ON 7 YGL157W (209011..210054) Oxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) YGL156W 1 210421 213672 ON 7 AMS1 (210421..213672) Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway YGL155W 1 214086 215216 ON 7 CDC43 (214086..215216) Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis YGL154C 0 215283 216101 ON 7 LYS5 complement(215283..216101) Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine YGL153W 1 216278 217303 ON 7 PEX14 (216278..217303) Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p YGL152C 0 216697 217374 OFF 7 YGL152C complement(216697..217374) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W YGL151W 1 217529 220927 ON 7 NUT1 (217529..220927) Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription YGL150C 0 221109 225578 ON 7 INO80 complement(221109..225578) ATPase that forms a large complex, containing actin and several actin-related proteins, that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; shows similarity to the Snf2p family of ATPases YGL149W 1 225577 225882 OFF 7 YGL149W (225577..225882) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. YGL148W 1 226404 227534 ON 7 ARO2 (226404..227534) Bifunctional chorismate synthase and flavin reductase, catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids YGL147C 0 227759 228334 ON 7 RPL9A complement(227759..228334) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins YGL146C 0 228756 229691 ON 7 YGL146C complement(228756..229691) Putative protein of unknown function, contains two putative transmembrane spans, shows no significant homology to any other known protein sequence, YGL146C is not an essential gene YGL145W 1 230248 232353 ON 7 TIP20 (230248..232353) Peripheral membrane protein required for fusion of COPI vesicles with the ER, prohibits back-fusion of COPII vesicles with the ER, may act as a sensor for vesicles at the ER membrane; interacts with Sec20p YGL144C 0 232455 234512 ON 7 ROG1 complement(232455..234512) Protein with putative serine active lipase domain YGL143C 0 234722 235963 ON 7 MRF1 complement(234722..235963) Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability YGL142C 0 236274 238124 ON 7 GPI10 complement(236274..238124) Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog YGL141W 1 238358 241090 ON 7 HUL5 (238358..241090) Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability YGL140C 0 241358 245017 ON 7 YGL140C complement(241358..245017) Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains YGL139W 1 245721 248129 ON 7 FLC3 (245721..248129) Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER YGL138C 0 248499 249536 ON 7 YGL138C complement(248499..249536) Putative protein of unknown function; has no significant sequence similarity to any known protein YGL137W 1 249874 252743 ON 7 SEC27 (249874..249891,250092..252743) Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP YGL136C 0 252902 253864 ON 7 MRM2 complement(252902..253864) Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ YGL135W 1 254646 255299 ON 7 RPL1B (254646..255299) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl1Ap and has similarity to E. coli L1 and rat L10a ribosomal proteins; rpl1a rpl1b double null mutation is lethal YGL134W 1 255668 256969 ON 7 PCL10 (255668..256969) Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate YGL133W 1 257712 261506 ON 7 ITC1 (257712..261506) Component of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1 YGL132W 1 261585 261920 OFF 7 YGL132W (261585..261920) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C YGL131C 0 261653 265864 ON 7 SNT2 complement(261653..265864) DNA binding protein with similarity to the S. pombe Snt2 protein YGL130W 1 266150 267529 ON 7 CEG1 (266150..267529) Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphophatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide YGL129C 0 267728 269194 ON 7 RSM23 complement(267728..269194) Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p YGL128C 0 269299 270150 ON 7 CWC23 complement(269299..270150) Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p YGL127C 0 270398 270781 ON 7 SOH1 complement(270398..270781) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits YGL126W 1 271003 272145 ON 7 SCS3 (271003..272145) Protein required for inositol prototrophy, appears to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis YGL125W 1 272526 274328 ON 7 MET13 (272526..274328) Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway YGL124C 0 274786 276720 ON 7 MON1 complement(274786..276720) Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate YGL123W 1 277623 278387 ON 7 RPS2 (277623..278387) Protein component of the small (40S) subunit, essential for control of translational accuracy; has similarity to E. coli S5 and rat S2 ribosomal proteins YGL123C-A 0 277640 277870 OFF 7 YGL123C-A complement(277640..277870) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene RPS2/YGL123W YGL122C 0 278952 280529 ON 7 NAB2 complement(278952..280529) Nuclear polyadenylated RNA-binding protein; autoregulates mRNA levels; related to human hnRNPs; has nuclear localization signal sequence that binds to Kap104p; required for poly(A) tail length control and nuclear mRNA export YGL121C 0 280783 281163 ON 7 GPG1 complement(280783..281163) Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Grp1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p YGL120C 0 281640 283943 ON 7 PRP43 complement(281640..283943) RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome YGL119W 1 284448 285953 ON 7 ABC1 (284448..285953) Protein required for ubiquinone (coenzyme Q) biosynthesis and for respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis YGL118C 0 288020 288457 OFF 7 YGL118C complement(288020..288457) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL117W 1 288518 289315 ON 7 YGL117W (288518..289315) Putative protein of unknown function YGL116W 1 289812 291644 ON 7 CDC20 (289812..291644) Cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C), which is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate YGL115W 1 292036 293004 ON 7 SNF4 (292036..293004) Activating gamma subunit of the AMP-activated Snf1p kinase complex (contains Snf1p and a Sip1p/Sip2p/Gal83p family member); activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis YGL114W 1 293463 295640 ON 7 YGL114W (293463..295640) Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters YGL113W 1 295935 297941 ON 7 SLD3 (295935..297941) Protein involved in the initiation of DNA replication, required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p YGL112C 0 298181 299731 ON 7 TAF6 complement(298181..299731) Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 YGL111W 1 299981 301372 ON 7 NSA1 (299981..301372) Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis YGL110C 0 301540 303414 ON 7 CUE3 complement(301540..303414) Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination YGL109W 1 303516 303839 OFF 7 YGL109W (303516..303839) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C YGL108C 0 303652 304074 ON 7 YGL108C complement(303652..304074) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YGL107C 0 304336 306276 ON 7 RMD9 complement(304336..306276) Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes YGL106W 1 306563 307012 ON 7 MLC1 (306563..307012) Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition YGL105W 1 307440 308570 ON 7 ARC1 (307440..308570) Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids YGL104C 0 308716 310176 ON 7 VPS73 complement(308716..310176) Mitochondrial protein involved in vacuolar protein sorting; putative transporter; member of the sugar porter family YGL103W 1 310970 311930 ON 7 RPL28 (310970..311018,311530..311930) Ribosomal protein L29 of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a ribosomal proteins; may have peptidyl transferase activity; can mutate to cycloheximide resistance YGL102C 0 311508 311936 OFF 7 YGL102C complement(311508..311936) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein YGL101W 1 312196 312843 ON 7 YGL101W (312196..312843) Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p YGL100W 1 313237 314286 ON 7 SEH1 (313237..314286) Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nup120p, Nup145p, and Seh1p); homologous to Sec13p YGL099W 1 314634 316556 ON 7 LSG1 (314634..316556) Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm YGL098W 1 317345 318082 ON 7 USE1 (317345..318082) Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p YGL097W 1 321785 323233 ON 7 SRM1 (321785..323233) Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p YGL096W 1 325334 326164 ON 7 TOS8 (325334..326164) Homeodomain-containing transcription factor; SBF regulated target gene that in turn regulates expression of genes involved in G1/S phase events such as bud site selection, bud emergence and cell cycle progression; similarity to Cup9p YGL095C 0 328877 330610 ON 7 VPS45 complement(328877..330610) Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment YGL094C 0 331121 334468 ON 7 PAN2 complement(331121..334468) Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes YGL093W 1 334889 337642 ON 7 SPC105 (334889..337642) Protein required for accurate chromosome segregation, localizes to the nuclear side of the spindle pole body; forms a complex with Ydr532cp YGL092W 1 337909 341862 ON 7 NUP145 (337909..341862) Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p YGL091C 0 342059 343045 ON 7 NBP35 complement(342059..343045) Essential P-loop NTPase that functions in nuclear and cytosolic iron-sulfur (FeS) protein assembly; has a highly conserved N-terminus YGL090W 1 343322 344587 ON 7 LIF1 (343322..344587) Protein involved in DNA double-strand break repair; physically interacts with DNA ligase 4 (Lig4p); homologous to mammalian XRCC4 protein YGL089C 0 344794 345156 ON 7 MF(ALPHA)2 complement(344794..345156) Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 YGL088W 1 345839 346204 OFF 7 YGL088W (345839..346204) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing YGL087C 0 346409 346907 ON 7 MMS2 complement(346409..346811,346897..346907) Protein involved in error-free postreplication DNA repair; forms a heteromeric complex with Ubc13p that has a ubiquitin-conjugating activity; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p YGL086W 1 347122 349371 ON 7 MAD1 (347122..349371) Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p YGL085W 1 349619 350443 ON 7 YGL085W (349619..350443) Putative protein of unknown function, has homology to Staphylococcus aureus nuclease; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS YGL084C 0 350619 352301 ON 7 GUP1 complement(350619..352301) Plasma membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases YGL083W 1 353061 355475 ON 7 SCY1 (353061..355475) Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase YGL082W 1 355830 356975 ON 7 YGL082W (355830..356975) Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene YGL081W 1 357380 358342 ON 7 YGL081W (357380..358342) Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis YGL080W 1 358639 359031 ON 7 YGL080W (358639..359031) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL079W 1 359447 360103 ON 7 YGL079W (359447..360103) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome; identified as a transcriptional activator in a high-throughput yeast one-hybrid assay YGL078C 0 360291 361862 ON 7 DBP3 complement(360291..361862) Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis YGL077C 0 362228 363919 ON 7 HNM1 complement(362228..363919) Choline transporter (permease) that also controls the uptake of nitrogen mustard; expression is co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol YGL076C 0 364338 365999 ON 7 RPL7A complement(364338..364967,365436..365529,365989..365999) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2) YGL075C 0 366928 368091 ON 7 MPS2 complement(366928..368091) Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p YGL074C 0 368599 368925 OFF 7 YGL074C complement(368599..368925) Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor YGL073W 1 368756 371257 ON 7 HSF1 (368756..371257) Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated YGL072C 0 371006 371365 OFF 7 YGL072C complement(371006..371365) Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1 YGL071W 1 372015 374087 ON 7 AFT1 (372015..374087) Transcription factor involved in iron utilization and homeostasis; binds the consensus site PyPuCACCCPu and activates the expression of target genes in response to changes in iron availability YGL070C 0 374462 374830 ON 7 RPB9 complement(374462..374830) RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site; involved in telomere maintenance YGL069C 0 375053 375517 OFF 7 YGL069C complement(375053..375517) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance YGL068W 1 375090 375674 ON 7 MNP1 (375090..375674) Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth YGL067W 1 376104 377258 ON 7 NPY1 (376104..377258) NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes YGL066W 1 377612 379585 ON 7 SGF73 (377612..379585) 73 kDa subunit of SAGA histone acetyltransferase complex; involved in formation of the preinitiation complex assembly at promoters YGL065C 0 379763 381274 ON 7 ALG2 complement(379763..381274) Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol YGL064C 0 381526 383211 ON 7 MRH4 complement(381526..383211) Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function YGL063W 1 383657 384769 ON 7 PUS2 (383657..384769) Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification YGL063C-A 0 384505 384654 OFF 7 YGL063C-A complement(384505..384654) Dubious open reading frame, unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YGL062W 1 385199 388735 ON 7 PYC1 (385199..388735) Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly similar to isoform Pyc2p but differentially regulated; mutations in the human homolog are associated with lactic acidosis YGL061C 0 388971 389714 ON 7 DUO1 complement(388971..389714) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YGL060W 1 390070 391995 ON 7 YBP2 (390070..391995) Protein with a role in resistance to oxidative stress; has similarity to Ybp1p, which is involved in regulation of the transcription factor Yap1p via oxidation of specific cysteine residues YGL059W 1 392228 393703 ON 7 YGL059W (392228..393703) Protein kinase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL058W 1 393992 394510 ON 7 RAD6 (393992..394510) Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) YGL057C 0 394972 395835 ON 7 YGL057C complement(394972..395835) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant exhibits a growth defect on a non-fermentable (respiratory) carbon source YGL056C 0 396041 397624 ON 7 SDS23 complement(396041..397624) One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation YGL055W 1 398631 400163 ON 7 OLE1 (398631..400163) Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria YGL054C 0 400874 401290 ON 7 ERV14 complement(400874..401290) Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon YGL053W 1 402592 403305 ON 7 PRM8 (402592..403305) Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family YGL052W 1 403440 403745 OFF 7 YGL052W (403440..403745) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MST27 YGL051W 1 403690 404394 ON 7 MST27 (403690..404394) Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles YGL050W 1 405779 406600 ON 7 TYW3 (405779..406600) Protein required for the synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions YGL049C 0 406863 409607 ON 7 TIF4632 complement(406863..409607) Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p YGL048C 0 410072 411289 ON 7 RPT6 complement(410072..411289) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle YGL047W 1 411555 412163 ON 7 ALG13 (411555..412163) Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases YGL045W 1 414106 415734 ON 7 RIM8 (414106..415734) Protein of unknown function, involved in the proteolytic activation of Rim101p in response to alkaline pH; has similarity to A. nidulans PalF YGL044C 0 416150 417040 ON 7 RNA15 complement(416150..417040) Cleavage and polyadenylation factor I (CF I) component involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p YGL043W 1 417487 418416 ON 7 DST1 (417487..418416) General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites YGL042C 0 418292 418597 OFF 7 YGL042C complement(418292..418597) Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 YGL041C-B 0 418709 418891 OFF 7 YGL041C-B complement(418709..418891) Putative protein of unknown function; identified by fungal homology and RT-PCR YGL041C 0 418917 419231 OFF 7 YGL041C complement(418917..419231) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL041W-A 1 419047 419295 OFF 7 YGL041W-A (419047..419295) Dubious open reading frame unlikely to encode a protein; partially overlaps the dubious ORF YGL041C; identified by expression profiling and mass spectrometry YGL040C 0 419533 420561 ON 7 HEM2 complement(419533..420561) Delta-aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of delta-aminolevulinic acid to porphobilinogen, the second step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus YGL039W 1 423967 425013 ON 7 YGL039W (423967..425013) Oxidoreductase, catalyzes NADPH-dependent reduction of the bicyclic diketone bicyclo[2.2.2]octane-2,6-dione (BCO2,6D) to the chiral ketoalcohol (1R,4S,6S)-6-hydroxybicyclo[2.2.2]octane-2-one (BCO2one6ol) YGL038C 0 425368 426810 ON 7 OCH1 complement(425368..426810) Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins YGL037C 0 427303 427953 ON 7 PNC1 complement(427303..427953) Nicotinamidase that converts nicotinamide to nicotinic acid as part of the NAD(+) salvage pathway, required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span YGL036W 1 428606 431335 ON 7 YGL036W (428606..431335) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene YGL035C 0 431553 433067 ON 7 MIG1 complement(431553..433067) Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase YGL034C 0 433219 433584 OFF 7 YGL034C complement(433219..433584) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGL033W 1 435630 436356 ON 7 HOP2 (435630..435684,435755..436356) Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair YGL032C 0 436575 436838 ON 7 AGA2 complement(436575..436838) Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds YGL031C 0 437472 437939 ON 7 RPL24A complement(437472..437939) Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate YGL030W 1 439096 439643 ON 7 RPL30 (439096..439098,439329..439643) Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript YGL029W 1 440068 440430 ON 7 CGR1 (440068..440430) Protein involved in nucleolar integrity and processing of the pre-rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress YGL028C 0 441286 442914 ON 7 SCW11 complement(441286..442914) Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p YGL027C 0 443647 446148 ON 7 CWH41 complement(443647..446148) Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress YGL026C 0 446417 448540 ON 7 TRP5 complement(446417..448540) Tryptophan synthase involved in tryptophan biosynthesis, regulated by the general control system of amino acid biosynthesis YGL025C 0 448769 449962 ON 7 PGD1 complement(448769..449962) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor YGL024W 1 449793 450128 OFF 7 YGL024W (449793..450128) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C YGL023C 0 450202 452109 ON 7 PIB2 complement(450202..452109) Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene expression; similar to Fab1 and Vps27 YGL022W 1 452409 454565 ON 7 STT3 (452409..454565) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis YGL021W 1 454790 457072 ON 7 ALK1 (454790..457072) Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; similar to Alk2p and to mammalian haspins YGL020C 0 457168 457875 ON 7 GET1 complement(457168..457875) Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance YGL019W 1 458161 458997 ON 7 CKB1 (458161..458997) Beta regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB2, the many substrates include transcription factors and all RNA polymerases YGL018C 0 459115 459669 ON 7 JAC1 complement(459115..459669) Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix YGL017W 1 459859 461370 ON 7 ATE1 (459859..461370) Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway YGL016W 1 461671 464916 ON 7 KAP122 (461671..464916) Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance YGL015C 0 465048 465440 ON 7 YGL015C complement(465048..465440) Hypothetical protein YGL014W 1 466146 468812 ON 7 PUF4 (466146..468812) Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors YGL014C-A 0 466242 466403 OFF 7 YGL014C-A complement(466242..466403) Dubious open reading frame, unlikely to encode a functional protein; identified by expression profiling and mass spectrometry YGL013C 0 469097 472303 ON 7 PDR1 complement(469097..472303) Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes YGL012W 1 472860 474281 ON 7 ERG4 (472860..474281) C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol YGL011C 0 474494 475252 ON 7 SCL1 complement(474494..475252) Alpha subunit of the 20S core complex of the 26S proteasome involved in the degradation of ubiquitinated substrates; essential for growth; detected in the mitochondria YGL010W 1 475550 476074 ON 7 YGL010W (475550..476074) Putative protein of unknown function; YGL010W is not an essential gene YGL009C 0 476318 478657 ON 7 LEU1 complement(476318..478657) Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway YGL008C 0 479915 482671 ON 7 PMA1 complement(479915..482671) Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases YGL007C-A 0 482946 483032 OFF 7 YGL007C-A complement(482946..483032) Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; haploid deletion strain exhibits a slow-growth phenotype YGL007W 1 483265 483642 OFF 7 BRP1 (483265..483642) Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 YGL006W-A 1 485428 485538 OFF 7 YGL006W-A (485428..485538) Putative protein of unknown function; identified by SAGE YGL006W 1 485925 489446 ON 7 PMC1 (485925..489446) Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a YGL005C 0 489712 490551 ON 7 COG7 complement(489712..490551) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YGL004C 0 490707 491960 ON 7 RPN14 complement(490707..491960) Putative non-ATPase subunit of the 19S regulatory particle of the 26S proteasome; localized to the cytoplasm YGL003C 0 492478 494178 ON 7 CDH1 complement(492478..494178) Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including CDC20, ASE1, CIN8 and FIN1 YGL002W 1 494521 495171 ON 7 ERP6 (494521..495171) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGL001C 0 495457 496506 ON 7 ERG26 complement(495457..496506) C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis YGR001C 0 497137 498038 ON 7 YGR001C complement(497137..497369,497463..497941,498004..498038) Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae YGR002C 0 498480 499910 ON 7 SWC4 complement(498480..499910) Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex YGR003W 1 500136 502370 ON 7 CUL3 (500136..502370) Ubiquitin-protein ligase, member of the cullin family with similarity to Cdc53p and human CUL3; required for ubiquitin-dependent degredation of the RNA Polymerase II subunit RPO21 YGR004W 1 502942 504330 ON 7 PEX31 (502942..504330) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p YGR005C 0 504661 505863 ON 7 TFG2 complement(504661..505863) TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 YGR006W 1 506074 506829 ON 7 PRP18 (506074..506829) Splicing factor involved in the positioning of the 3' splice site during the second catalytic step of splicing, part of snRNP U5, interacts with Slu7p YGR007W 1 506973 507944 ON 7 MUQ1 (506973..507944) Choline phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases YGR008C 0 508114 508368 ON 7 STF2 complement(508114..508368) Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein YGR009C 0 509051 511006 ON 7 SEC9 complement(509051..511006) t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog YGR010W 1 511549 512736 ON 7 NMA2 (511549..512736) Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway YGR011W 1 512501 512827 OFF 7 YGR011W (512501..512827) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR012W 1 513163 514344 ON 7 YGR012W (513163..514344) Putative cysteine synthase, localized to the mitochondrial outer membrane YGR013W 1 514558 516420 ON 7 SNU71 (514558..516420) Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart YGR014W 1 516947 520867 ON 7 MSB2 (516947..520867) Mucin family member involved in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate YGR015C 0 521094 522080 ON 7 YGR015C complement(521094..522080) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YGR016W 1 522265 522837 ON 7 YGR016W (522265..522837) Putative protein of unknown function YGR017W 1 523791 524684 ON 7 YGR017W (523791..524684) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm YGR018C 0 524369 524698 OFF 7 YGR018C complement(524369..524698) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR017W YGR019W 1 525233 526648 ON 7 UGA1 (525233..526648) Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization YGR020C 0 526977 527333 ON 7 VMA7 complement(526977..527333) Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane YGR021W 1 527636 528508 ON 7 YGR021W (527636..528508) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR022C 0 529261 529590 OFF 7 YGR022C complement(529261..529590) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W YGR023W 1 529268 530923 ON 7 MTL1 (529268..530923) Protein with both structural and functional similarity to Mid2p, which is a plasma membrane sensor required for cell integrity signaling during pheromone-induced morphogenesis; suppresses rgd1 null mutations YGR024C 0 531887 532600 ON 7 THG1 complement(531887..532600) tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes YGR025W 1 532643 532945 OFF 7 YGR025W (532643..532945) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR026W 1 532989 533825 ON 7 YGR026W (532989..533825) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YGR027C 0 534136 534462 ON 7 RPS25A complement(534136..534462) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similarity to rat S25 ribosomal protein YGR028W 1 542207 543295 ON 7 MSP1 (542207..543295) Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase YGR029W 1 543557 544209 ON 7 ERV1 (543557..543642,543726..544209) Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) YGR030C 0 545685 546161 ON 7 POP6 complement(545685..546161) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends YGR031W 1 546445 547473 ON 7 YGR031W (546445..547473) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR031C-A 0 546813 547304 OFF 7 YGR031C-A complement(546813..547304) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YGR031W YGR032W 1 548268 553955 ON 7 GSC2 (548268..553955) Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) YGR033C 0 554252 554971 ON 7 TIM21 complement(554252..554971) Constituent of the mitochondrial inner membrane presequence translocase (TIM23 complex); may regulate protein import by binding to both the translocase of the outer membrane (TOM) and presequence-associated motor (PAM) complexes YGR034W 1 555816 556676 ON 7 RPL26B (555816..555834,556312..556676) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Ap and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA YGR035C 0 557076 557426 ON 7 YGR035C complement(557076..557426) Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance YGR035W-A 1 557569 557790 OFF 7 YGR035W-A (557569..557790) Putative protein of unknown function YGR036C 0 558153 558872 ON 7 CAX4 complement(558153..558872) Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation YGR037C 0 559735 559998 ON 7 ACB1 complement(559735..559998) Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes YGR038W 1 560686 561354 ON 7 ORM1 (560686..561354) Evolutionarily conserved protein with similarity to Orm2p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum YGR039W 1 574892 575203 OFF 7 YGR039W (574892..575203) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 YGR040W 1 575402 576508 ON 7 KSS1 (575402..576508) Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains YGR041W 1 577491 579134 ON 7 BUD9 (577491..579134) Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole YGR042W 1 579480 580295 ON 7 YGR042W (579480..580295) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR043C 0 580439 581440 ON 7 YGR043C complement(580439..581440) Protein of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; null mutant non-quiescent cells exhibit reduced reproductive capacity YGR044C 0 582994 583896 ON 7 RME1 complement(582994..583896) Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-a2 regulator; mediates cell type control of sporulation YGR045C 0 583938 584300 OFF 7 YGR045C complement(583938..584300) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR046W 1 584899 586056 ON 7 TAM41 (584899..586056) Mitochondrial protein involved in protein import into the mitochondrial matrix; maintains the functional integrity of the TIM23 protein translocator complex; viability of null mutant is strain-dependent; mRNA is targeted to the bud YGR047C 0 586396 589473 ON 7 TFC4 complement(586396..589473) One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 YGR048W 1 589830 590915 ON 7 UFD1 (589830..590915) Protein that interacts with Cdc48p and Npl4p, involved in recognition of polyubiquitinated proteins and their presentation to the 26S proteasome for degradation; involved in transporting proteins from the ER to the cytosol YGR049W 1 591318 591881 ON 7 SCM4 (591318..591881) Potential regulatory effector of CDC4 function, suppresses a temperature-sensitive allele of CDC4, tripartite protein structure in which a charged region separates two uncharged domains, not essential for mitosis or meiosis YGR050C 0 592090 592446 OFF 7 YGR050C complement(592090..592446) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR051C 0 592910 593233 OFF 7 YGR051C complement(592910..593233) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene YGR052W 1 593600 594709 ON 7 YGR052W (593600..594709) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR053C 0 594990 595841 ON 7 YGR053C complement(594990..595841) Putative protein of unknown function YGR054W 1 596697 598625 ON 7 YGR054W (596697..598625) Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A YGR055W 1 599421 601145 ON 7 MUP1 (599421..601145) High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake YGR056W 1 601665 604451 ON 7 RSC1 (601665..604451) Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook YGR057C 0 604777 605505 ON 7 LST7 complement(604777..605505) Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface YGR058W 1 606140 607147 ON 7 PEF1 (606140..607147) Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2 YGR059W 1 607567 609105 ON 7 SPR3 (607567..609105) Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI YGR060W 1 610568 611497 ON 7 ERG25 (610568..611497) C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol YGR061C 0 611893 615969 ON 7 ADE6 complement(611893..615969) Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway YGR062C 0 616332 617282 ON 7 COX18 complement(616332..617282) Mitochondrial inner membrane protein, required for export of the Cox2p C terminus from the mitochondrial matrix to the intermembrane space during its assembly into cytochrome c oxidase; similar to Oxa2p of N. crassa YGR063C 0 617520 617828 ON 7 SPT4 complement(617520..617828) Protein that forms a complex with Spt5p and mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing; in addition, Spt4p is involved in kinetochore function and gene silencing YGR064W 1 617625 617993 OFF 7 YGR064W (617625..617993) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C YGR065C 0 618081 619862 ON 7 VHT1 complement(618081..619862) High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin YGR066C 0 620925 621803 ON 7 YGR066C complement(620925..621803) Putative protein of unknown function YGR067C 0 622406 624790 ON 7 YGR067C complement(622406..624790) Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p YGR068C 0 625328 627088 ON 7 YGR068C complement(625328..627088) Putative protein of unknown function; YGR068C is not an essential gene. YGR068W-A 1 626298 626393 OFF 7 YGR068W-A (626298..626393) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with the uncharacterized ORF YGR068C YGR069W 1 627087 627422 OFF 7 YGR069W (627087..627422) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR070W 1 627810 631277 ON 7 ROM1 (627810..631277) GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP YGR071C 0 631485 634067 ON 7 YGR071C complement(631485..634067) Putative protein of unknown function; deletion mutant has increased glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YGR072W 1 634308 635471 ON 7 UPF3 (634308..635471) Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance YGR073C 0 635619 635990 OFF 7 YGR073C complement(635619..635990) Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein YGR074W 1 635716 636156 ON 7 SMD1 (635716..636156) Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1 YGR075C 0 636151 636879 ON 7 PRP38 complement(636151..636879) Unique component of the U4/U6.U5 tri-snRNP particle, dispensable for spliceosome assembly, but required for conformational changes which lead to catalytic activation of the spliceosome YGR076C 0 637108 637581 ON 7 MRPL25 complement(637108..637581) Mitochondrial ribosomal protein of the large subunit YGR077C 0 637748 639517 ON 7 PEX8 complement(637748..639517) Intraperoxisomal organizer of the peroxisomal import machinery, tightly associated with the lumenal face of the peroxisomal membrane, essential for peroxisome biogenesis, binds PTS1-signal receptor Pex5p YGR078C 0 639776 640375 ON 7 PAC10 complement(639776..640375) Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding YGR079W 1 640724 641836 ON 7 YGR079W (640724..641836) Putative protein of unknown function; YGR079W is not an essential gene YGR080W 1 642014 643012 ON 7 TWF1 (642014..643012) Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly YGR081C 0 643152 643784 ON 7 SLX9 complement(643152..643784) Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant YGR082W 1 644048 644599 ON 7 TOM20 (644048..644599) Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins YGR083C 0 644864 646819 ON 7 GCD2 complement(644864..646819) Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YGR084C 0 647127 648146 ON 7 MRP13 complement(647127..648146) Mitochondrial ribosomal protein of the small subunit YGR085C 0 648387 648911 ON 7 RPL11B complement(648387..648911) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of proteins and RNA of the 60S subunit; has similarity to E. coli L5 and rat L11 YGR086C 0 649602 650621 ON 7 PIL1 complement(649602..650621) Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Lsp1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways YGR087C 0 651294 652985 ON 7 PDC6 complement(651294..652985) Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, involved in amino acid catabolism YGR088W 1 654638 656326 ON 7 CTT1 (654638..656326) Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide YGR089W 1 656964 659774 ON 7 NNF2 (656964..659774) Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation YGR090W 1 662362 666075 ON 7 UTP22 (662362..666075) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YGR091W 1 666345 667829 ON 7 PRP31 (666345..667829) Splicing factor, component of the U4/U6-U5 snRNP complex YGR092W 1 668193 669911 ON 7 DBF2 (668193..669911) Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis YGR093W 1 670392 671915 ON 7 YGR093W (670392..671915) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YGR094W 1 672190 675504 ON 7 VAS1 (672190..675504) Mitochondrial and cytoplasmic valyl-tRNA synthetase YGR095C 0 675675 676346 ON 7 RRP46 complement(675675..676346) Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex YGR096W 1 676625 677569 ON 7 TPC1 (676625..677569) Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family YGR097W 1 678699 682139 ON 7 ASK10 (678699..682139) Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p, which relieves repression of stress-response genes YGR098C 0 682570 687462 ON 7 ESP1 complement(682570..687462) Separase with cysteine protease activity (related to caspases) that promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibited by Pds1p YGR099W 1 687903 689969 ON 7 TEL2 (687903..689969) Essential DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect YGR100W 1 690249 693101 ON 7 MDR1 (690249..693101) Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function YGR101W 1 693367 694407 ON 7 PCP1 (693367..694407) Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases YGR102C 0 694588 695139 ON 7 YGR102C complement(694588..695139) Putative protein of unknown function; transposon insertion mutant is salt sensitive and deletion mutant has growth defects; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR103W 1 695421 697238 ON 7 NOP7 (695421..697238) Nucleolar protein involved in rRNA processing and 60S ribosomal subunit biogenesis; constituent of several different pre-ribosomal particles; required for exit from G0 and the initiation of cell proliferation YGR104C 0 697449 698372 ON 7 SRB5 complement(697449..698372) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in telomere maintenance YGR105W 1 698603 698836 ON 7 VMA21 (698603..698836) Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) YGR106C 0 698993 699790 ON 7 YGR106C complement(698993..699790) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar memebrane YGR107W 1 702671 703120 OFF 7 YGR107W (702671..703120) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR108W 1 703640 705055 ON 7 CLB1 (703640..705055) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome YGR109C 0 705363 706505 ON 7 CLB6 complement(705363..706505) B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 YGR110W 1 713713 715050 ON 7 YGR110W (713713..715050) Putative protein of unknown function; transcription is increased in response to genotoxic stress YGR111W 1 715832 717034 ON 7 YGR111W (715832..717034) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR112W 1 717362 718531 ON 7 SHY1 (717362..718531) Mitochondrial inner membrane protein required for normal respiration, possible chaperone involved in assembly of cytochrome c oxidase; similar to SURF1 from mammals, chickens, and D. melanogaster YGR113W 1 718897 719928 ON 7 DAM1 (718897..719928) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments YGR114C 0 720269 720658 OFF 7 YGR114C complement(720269..720658) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 YGR115C 0 720376 721155 OFF 7 YGR115C complement(720376..721155) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C YGR116W 1 720413 724768 ON 7 SPT6 (720413..724768) Essential protein that interacts with histones and is involved in nucleosome disassembly and reassembly during transcription elongation YGR117C 0 725053 726483 ON 7 YGR117C complement(725053..726483) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR118W 1 726978 727735 ON 7 RPS23A (726978..727042,727363..727735) Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; nearly identical to Rps23Bp and similar to E. coli S12 and rat S23 ribosomal proteins; deletion of both RPS23A and RPS23B is lethal YGR119C 0 728051 729676 ON 7 NUP57 complement(728051..729676) Nucleoporin, essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p YGR120C 0 730038 730826 ON 7 COG2 complement(730038..730826) Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YGR121C 0 731454 732932 ON 7 MEP1 complement(731454..732932) Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation YGR121W-A 1 733417 733632 OFF 7 YGR121W-A (733417..733632) Putative protein of unknown function YGR122W 1 733940 735148 ON 7 YGR122W (733940..735148) Putative protein of unknown function; deletion mutants do not properly process Rim101p and have decreased resistance to rapamycin; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR122C-A 0 735635 735763 OFF 7 YGR122C-A complement(735635..735763) Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W YGR123C 0 736667 738208 ON 7 PPT1 complement(736667..738208) Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth YGR124W 1 739949 741667 ON 7 ASN2 (739949..741667) Asparagine synthetase, isozyme of Asn1p; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway YGR125W 1 742330 745440 ON 7 YGR125W (742330..745440) Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YGR126W 1 745840 746532 ON 7 YGR126W (745840..746532) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS YGR127W 1 746803 747741 ON 7 YGR127W (746803..747741) Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response YGR128C 0 747955 750096 ON 7 UTP8 complement(747955..750096) Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YGR129W 1 750405 751052 ON 7 SYF2 (750405..751052) Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression YGR130C 0 751399 753849 ON 7 YGR130C complement(751399..753849) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) YGR131W 1 754731 755255 ON 7 YGR131W (754731..755255) Protein of unknown function; expression induced in response to ketoconazole; promoter region contains a sterol regulatory element motif, which has been identified as a Upc2p-binding site YGR132C 0 755594 756457 ON 7 PHB1 complement(755594..756457) Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation YGR133W 1 756901 757452 ON 7 PEX4 (756901..757452) Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis YGR134W 1 757775 761143 ON 7 CAF130 (757775..761143) Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation YGR135W 1 761397 762173 ON 7 PRE9 (761397..762173) 20S proteasome beta-type subunit; the only nonessential 20S subunit YGR136W 1 762433 763158 ON 7 LSB1 (762433..763158) Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YGR137W 1 762893 763267 OFF 7 YGR137W (762893..763267) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR138C 0 763767 765611 ON 7 TPO2 complement(763767..765611) Polyamine transport protein specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; member of the major facilitator superfamily YGR139W 1 765731 766069 OFF 7 YGR139W (765731..766069) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR140W 1 767434 770304 ON 7 CBF2 (767434..770304) Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo YGR141W 1 770574 771977 ON 7 VPS62 (770574..771977) Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins YGR142W 1 772459 773691 ON 7 BTN2 (772459..773691) v-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase YGR143W 1 775198 777513 ON 7 SKN1 (775198..777513) Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p YGR144W 1 780404 781384 ON 7 THI4 (780404..781384) Thiazole synthase, catalyzes formation of the thiazole moiety of thiamin pyrophosphate; required for thiamine biosynthesis and for mitochondrial genome stability YGR145W 1 781772 783895 ON 7 ENP2 (781772..783895) Essential nucleolar protein of unknown function; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p YGR146C 0 784228 784863 ON 7 YGR146C complement(784228..784863) Putative protein of unknown function; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of a temperature sensitive hsf1 mutant YGR146C-A 0 785281 785442 OFF 7 YGR146C-A complement(785281..785442) Putative protein of unknown function YGR147C 0 786059 786925 ON 7 NAT2 complement(786059..786925) N alpha-acetyl-transferase, transfers acetyl group from acetyl coenzyme A to the N-terminal methionine residues of proteins YGR148C 0 787317 787784 ON 7 RPL24B complement(787317..787784) Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Ap and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate YGR149W 1 789036 790334 ON 7 YGR149W (789036..790334) Putative protein of unknown function; predicted to be an integal membrane protein YGR150C 0 790464 793058 ON 7 YGR150C complement(790464..793058) Protein of unknown function, contains PPR motifs; mutant has growth defects on both non-fermentable carbon sources and rich medium; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR151C 0 794660 794995 OFF 7 YGR151C complement(794660..794995) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C YGR152C 0 794679 795497 ON 7 RSR1 complement(794679..795497) GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases YGR153W 1 796097 796750 ON 7 YGR153W (796097..796750) Putative protein of unknown function YGR154C 0 796803 797873 ON 7 GTO1 complement(796803..797873) Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization YGR155W 1 798548 800071 ON 7 CYS4 (798548..800071) Cystathionine beta-synthase, catalyzes the synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis YGR156W 1 800551 801828 ON 7 PTI1 (800551..801828) Pta1p Interacting protein YGR157W 1 802445 805054 ON 7 CHO2 (802445..805054) Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis YGR158C 0 805274 806026 ON 7 MTR3 complement(805274..806026) 3'5' exoribonuclease, exosome subunit; nucleolar protein involved in export of mRNA and ribosomal subunits; homologous to the E. coli exonuclease RNase PH YGR159C 0 806417 807661 ON 7 NSR1 complement(806417..807661) Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis YGR160W 1 807078 807689 OFF 7 YGR160W (807078..807689) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR161C 0 808634 809425 ON 7 RTS3 complement(808634..809425) Putative component of the protein phosphatase type 2A complex YGR161W-C 1 810232 810510 OFF 7 YGR161W-C (810232..810510) Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species YGR162W 1 824064 826922 ON 7 TIF4631 (824064..826922) Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p YGR163W 1 827557 828582 ON 7 GTR2 (827557..828582) Cytoplasmic GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; subunit of EGO complex, which is involved in microautophagy regulation, and of GSE complex, required for sorting of Gap1p; homolog of human RagC and RagD YGR164W 1 828630 828965 OFF 7 YGR164W (828630..828965) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR165W 1 829121 830158 ON 7 MRPS35 (829121..830158) Mitochondrial ribosomal protein of the small subunit YGR166W 1 830520 832202 ON 7 KRE11 (830520..832202) Protein involved in biosynthesis of cell wall beta-glucans; subunit of the TRAPP (transport protein particle) complex, which is involved in the late steps of endoplasmic reticulum to Golgi transport YGR167W 1 832461 833162 ON 7 CLC1 (832461..833162) Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function, two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component YGR168C 0 833357 834487 ON 7 YGR168C complement(833357..834487) Putative protein of unknown function; YGR168C is not an essential gene YGR169C 0 834694 835908 ON 7 PUS6 complement(834694..835908) Pseudouridine synthase responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability YGR169C-A 0 836392 836670 ON 7 YGR169C-A complement(836392..836670) Putative protein of unknown function YGR170W 1 837147 840563 ON 7 PSD2 (837147..840563) Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine YGR171C 0 840829 842556 ON 7 MSM1 complement(840829..842556) Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p YGR172C 0 842850 843596 ON 7 YIP1 complement(842850..843596) Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles YGR173W 1 843859 844965 ON 7 RBG2 (843859..844965) Protein with similarity to mammalian developmentally regulated GTP-binding protein YGR174C 0 845898 846410 ON 7 CBP4 complement(845898..846410) Mitochondrial protein required for assembly of ubiquinol cytochrome-c reductase complex (cytochrome bc1 complex); interacts with Cbp3p and function is partially redundant with that of Cbp3p YGR174W-A 1 846660 846746 OFF 7 YGR174W-A (846660..846746) Putative protein of unknown function YGR175C 0 846938 848428 ON 7 ERG1 complement(846938..848428) Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine YGR176W 1 848725 849072 OFF 7 YGR176W (848725..849072) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YGR177C 0 848834 850441 ON 7 ATF2 complement(848834..850441) Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important in brewing YGR178C 0 851052 853220 ON 7 PBP1 complement(851052..853220) Protein interacting with poly(A)-binding protein Pab1p; likely involved in controlling the extent of mRNA polyadenylation; forms a complex with Mkt1p that may regulate HO translation; interacts with Lsm12p in a copurification assay YGR179C 0 853680 854900 ON 7 OKP1 complement(853680..854900) Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly YGR180C 0 855269 856306 ON 7 RNR4 complement(855269..856306) Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits YGR181W 1 858292 858609 ON 7 TIM13 (858292..858609) Mitochondrial intermembrane space protein, forms a complex with TIm8p that mediates import and insertion of a subset of polytopic inner membrane proteins; may prevent aggregation of incoming proteins in a chaperone-like manner YGR182C 0 858556 858909 OFF 7 YGR182C complement(858556..858909) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W YGR183C 0 859068 859481 ON 7 QCR9 complement(859068..859265,859479..859481) Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex YGR184C 0 859906 865758 ON 7 UBR1 complement(859906..865758) Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle of the 26S proteasome YGR185C 0 866341 867525 ON 7 TYS1 complement(866341..867525) Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr YGR186W 1 867779 869986 ON 7 TFG1 (867779..869986) TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 YGR187C 0 870237 871421 ON 7 HGH1 complement(870237..871421) Protein of unknown function with similarity to human HMG1 and HMG2; localizes to the cytoplasm YGR188C 0 872049 875114 ON 7 BUB1 complement(872049..875114) Protein kinase that forms a complex with Mad1p and Bub3p that is crucial in the checkpoint mechanism required to prevent cell cycle progression into anaphase in the presence of spindle damage, associates with centromere DNA via Skp1p YGR189C 0 876674 878197 ON 7 CRH1 complement(876674..878197) Cell wall protein that functions in the transfer of chitin to beta(1-6)glucan, putative chitin transglycosidase YGR190C 0 880301 880666 OFF 7 YGR190C complement(880301..880666) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W YGR191W 1 880425 882236 ON 7 HIP1 (880425..882236) High-affinity histidine permease, also involved in the transport of manganese ions YGR192C 0 882817 883815 ON 7 TDH3 complement(882817..883815) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall YGR193C 0 884514 885746 ON 7 PDX1 complement(884514..885746) Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core YGR194C 0 886078 887880 ON 7 XKS1 complement(886078..887880) Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains YGR195W 1 888887 889627 ON 7 SKI6 (888887..889627) 3'-to-5' phosphorolytic exoribonuclease that is a subunit of the exosome; required for 3' processing of the 5.8S rRNA; involved in 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs YGR196C 0 889738 892191 ON 7 FYV8 complement(889738..892191) Protein of unknown function, required for survival upon exposure to K1 killer toxin YGR197C 0 892502 894145 ON 7 SNG1 complement(892502..894145) Protein involved in nitrosoguanidine (MNNG) resistance; expression is regulated by transcription factors involved in multidrug resistance YGR198W 1 894698 897151 ON 7 YPP1 (894698..897151) Cargo-transport protein involved in endocytosis; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene YGR199W 1 897507 899786 ON 7 PMT6 (897507..899786) Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases YGR200C 0 899909 902275 ON 7 ELP2 complement(899909..902275) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin YGR201C 0 902525 903202 ON 7 YGR201C complement(902525..903202) Putative protein of unknown function YGR202C 0 903479 904753 ON 7 PCT1 complement(903479..904753) Cholinephosphate cytidylyltransferase, also known as CTP:phosphocholine cytidylyltransferase, rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding YGR203W 1 905242 905688 ON 7 YGR203W (905242..905688) Putative protein with sequence similarity to protein tyrosine phosphatases of the CDC25-like phosphatase family; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus YGR204W 1 905939 908779 ON 7 ADE3 (905939..908779) Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine YGR204C-A 0 909066 909179 OFF 7 YGR204C-A complement(909066..909179) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YGR205W 1 909218 910090 ON 7 YGR205W (909218..910090) ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases YGR206W 1 910437 910742 ON 7 MVB12 (910437..910742) ESCRT-I subunit necessary for the efficient transport of cargo proteins to the vacuolar lumen; deletion mutant sensitive to rapamycin and nystatin; green fluorescent protein (GFP)-fusion protein localizes to endosome YGR207C 0 910848 911633 ON 7 YGR207C complement(910848..911633) Protein that interacts with frataxin (Yfh1p); putative homolog of mammalian electron transfer flavoprotein complex subunit ETF-beta; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR208W 1 911888 912817 ON 7 SER2 (911888..912817) Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source YGR209C 0 912918 913232 ON 7 TRX2 complement(912918..913232) Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance YGR210C 0 913508 914743 ON 7 YGR210C complement(913508..914743) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR211W 1 915246 916706 ON 7 ZPR1 (915246..916706) Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p) YGR212W 1 917045 918451 ON 7 SLI1 (917045..918451) N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin YGR213C 0 918517 919470 ON 7 RTA1 complement(918517..919470) Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions YGR214W 1 920580 921793 ON 7 RPS0A (920580..920669,921125..921793) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Bp; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal YGR215W 1 922180 922512 ON 7 RSM27 (922180..922512) Mitochondrial ribosomal protein of the small subunit YGR216C 0 922638 924467 ON 7 GPI1 complement(922638..924467) Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs YGR217W 1 924699 930818 ON 7 CCH1 (924699..930818) Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together YGR218W 1 932544 935798 ON 7 CRM1 (932544..935798) Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus YGR219W 1 936038 936379 OFF 7 YGR219W (936038..936379) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C YGR220C 0 936077 936886 ON 7 MRPL9 complement(936077..936886) Mitochondrial ribosomal protein of the large subunit YGR221C 0 937127 938995 ON 7 TOS2 complement(937127..938995) Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p YGR222W 1 939926 940807 ON 7 PET54 (939926..940807) Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet122p and Pet494p; also required for splicing of the COX1 intron AI5 beta; located in the mitochondrial inner membrane YGR223C 0 940872 942218 ON 7 HSV2 complement(940872..942218) Phosphatidylinositol 3,5-bisphosphate-binding protein, predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization YGR224W 1 942809 944650 ON 7 AZR1 (942809..944650) Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole YGR225W 1 945148 947022 ON 7 AMA1 (945148..946330,946424..947022) Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis YGR226C 0 946795 947004 OFF 7 YGR226C complement(946795..947004) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W YGR227W 1 947423 949000 ON 7 DIE2 (947423..949000) Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 YGR228W 1 949368 949712 OFF 7 YGR228W (949368..949712) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C YGR229C 0 949385 950902 ON 7 SMI1 complement(949385..950902) Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity YGR230W 1 951897 952310 ON 7 BNS1 (951897..952310) Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis YGR231C 0 952551 953483 ON 7 PHB2 complement(952551..953483) Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation YGR232W 1 953963 954649 ON 7 NAS6 (953963..954649) Regulatory, non-ATPase subunit of the 26S proteasome; homolog of the human oncoprotein gankyrin, which interacts with the retinoblastoma tumor suppressor (Rb) and cyclin-dependent kinase 4/6 YGR233C 0 954677 958213 ON 7 PHO81 complement(954677..958213) Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p YGR234W 1 959908 961107 ON 7 YHB1 (959908..961107) Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses YGR235C 0 961364 962065 ON 7 YGR235C complement(961364..962065) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR236C 0 962534 962821 ON 7 SPG1 complement(962534..962821) Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR237C 0 963303 965660 ON 7 YGR237C complement(963303..965660) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR238C 0 966044 968692 ON 7 KEL2 complement(966044..968692) Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate YGR239C 0 969192 970058 ON 7 PEX21 complement(969192..970058) Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex18p YGR240C 0 970776 973739 ON 7 PFK1 complement(970776..973739) Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes YGR240C-A 0 974582 974782 OFF 7 YGR240C-A complement(974582..974782) Putative protein of unknown function; identified by fungal homology and RT-PCR YGR241C 0 974880 976586 ON 7 YAP1802 complement(974880..976586) Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1801p, member of the AP180 protein family YGR242W 1 976420 976728 OFF 7 YGR242W (976420..976728) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C YGR243W 1 977341 977781 ON 7 YGR243W (977341..977781) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YGR244C 0 978041 979324 ON 7 LSC2 complement(978041..979324) Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate YGR245C 0 979770 982073 ON 7 SDA1 complement(979770..982073) Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis YGR246C 0 982487 984277 ON 7 BRF1 complement(982487..984277) TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB YGR247W 1 984971 985690 ON 7 CPD1 (984971..985690) Cyclic nucleotide phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; no detectable phenotype is conferred by null mutation or by overexpression YGR248W 1 985977 986744 ON 7 SOL4 (985977..986744) 6-phosphogluconolactonase with similarity to Sol3p YGR249W 1 988054 989424 ON 7 MGA1 (988054..989424) Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants YGR250C 0 991181 993526 ON 7 YGR250C complement(991181..993526) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YGR251W 1 995644 996234 ON 7 YGR251W (995644..996234) Putative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the nucleolus; YGR251W is an essential gene YGR252W 1 996874 998193 ON 7 GCN5 (996874..998193) Histone acetyltransferase, acetylates N-terminal lysines on histones H2B and H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily YGR253C 0 998363 999145 ON 7 PUP2 complement(998363..999145) Alpha subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta YGR254W 1 1000932 1002245 ON 7 ENO1 (1000932..1002245) Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose YGR255C 0 1002528 1003967 ON 7 COQ6 complement(1002528..1003967) Putative flavin-dependent monooxygenase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes YGR256W 1 1004630 1006108 ON 7 GND2 (1004630..1006108) 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone YGR257C 0 1006211 1007311 ON 7 MTM1 complement(1006211..1007311) Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor YGR258C 0 1007677 1010772 ON 7 RAD2 complement(1007677..1010772) Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein YGR259C 0 1012487 1012927 OFF 7 YGR259C complement(1012487..1012927) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W YGR260W 1 1012491 1014095 ON 7 TNA1 (1012491..1014095) High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) YGR261C 0 1014327 1016756 ON 7 APL6 complement(1014327..1016756) Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools YGR262C 0 1016980 1017765 ON 7 BUD32 complement(1016980..1017765) Protein kinase proposed to be involved in bud-site selection, telomere uncapping and elongation, and transcription; component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Gon7p YGR263C 0 1017973 1019247 ON 7 YGR263C complement(1017973..1019247) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YGR264C 0 1019604 1021859 ON 7 MES1 complement(1019604..1021859) Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs YGR265W 1 1021654 1022064 OFF 7 YGR265W (1021654..1022064) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase YGR266W 1 1022662 1024767 ON 7 YGR266W (1022662..1024767) Protein of unknown function, predicted to contain a single transmembrane domain; localized to both the mitochondrial outer membrane and the plasma membrane YGR267C 0 1025010 1025741 ON 7 FOL2 complement(1025010..1025741) GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway YGR268C 0 1026063 1026659 ON 7 HUA1 complement(1026063..1026659) Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly YGR269W 1 1026642 1026968 OFF 7 YGR269W (1026642..1026968) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C YGR270W 1 1027376 1031515 ON 7 YTA7 (1027376..1031515) Protein of unknown function, member of CDC48/PAS1/SEC18 family of ATPases, potentially phosphorylated by Cdc28p YGR270C-A 0 1027388 1027606 OFF 7 YGR270C-A complement(1027388..1027606) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF YTA7/YGR270W YGR271W 1 1031797 1037700 ON 7 SLH1 (1031797..1037700) Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses YGR271C-A 0 1037806 1037997 OFF 7 YGR271C-A complement(1037806..1037997) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus YGR272C 0 1038048 1038506 ON 7 YGR272C complement(1038048..1038506) Putative protein of unknown function; deletion mutant has defects in pre-rRNA processing; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YGR273C 0 1038720 1039244 ON 7 YGR273C complement(1038720..1039244) Putative protein of unknown function; deletion mutant has no readily detectable phenotype YGR274C 0 1039901 1043101 ON 7 TAF1 complement(1039901..1043101) TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation, has histone acetyltransferase activity, involved in promoter binding and G1/S progression YGR275W 1 1043282 1043755 ON 7 RTT102 (1043282..1043755) Component of both the SWI/SNF and RSC chromatin remodeling complexes, suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition YGR276C 0 1043825 1045486 ON 7 RNH70 complement(1043825..1045486) 3' exoribonuclease, required for 5S rRNA and tRNA-Arg3 maturation YGR277C 0 1045650 1046567 ON 7 YGR277C complement(1045650..1046567) Putative pantetheine-phosphate adenylyltransferase (PPAT) that catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; conserved in bacteria, humans, and plants; essential for viability YGR278W 1 1046737 1048470 ON 7 CWC22 (1046737..1048470) Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data YGR279C 0 1048804 1049964 ON 7 SCW4 complement(1048804..1049964) Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating YGR280C 0 1050916 1051731 ON 7 PXR1 complement(1050916..1051731) Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain YGR281W 1 1052830 1057263 ON 7 YOR1 (1052830..1057263) Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin YGR282C 0 1057789 1058730 ON 7 BGL2 complement(1057789..1058730) Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance YGR283C 0 1059021 1060046 ON 7 YGR283C complement(1059021..1060046) Protein of unknown function; may interact with ribosomes, based on co-purification experiments; deletion mutant is resistant to fluconazole; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus YGR284C 0 1060664 1061596 ON 7 ERV29 complement(1060664..1061596) Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo YGR285C 0 1061858 1063159 ON 7 ZUO1 complement(1061858..1063159) Cytosolic ribosome-associated chaperone that acts, together with Ssz1p and the Ssb proteins, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p YGR286C 0 1063819 1064946 ON 7 BIO2 complement(1063819..1064946) Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant YGR287C 0 1067228 1068997 ON 7 YGR287C complement(1067228..1068997) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to alpha-D-glucosidase (maltase); authentic, non-tagged protein detected in purified mitochondria in high-throughput studies YGR288W 1 1070299 1071720 ON 7 MAL13 (1070299..1071720) MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C YGR289C 0 1073969 1075819 ON 7 MAL11 complement(1073969..1075819) Maltose permease, inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters YGR290W 1 1075488 1075931 OFF 7 YGR290W (1075488..1075931) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) YGR291C 0 1076289 1076510 OFF 7 YGR291C complement(1076289..1076510) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YGR292W 1 1076605 1078359 ON 7 MAL12 (1076605..1078359) Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus YGR293C 0 1079891 1080352 OFF 7 YGR293C complement(1079891..1080352) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YGR294W YGR294W 1 1080312 1080674 ON 7 YGR294W (1080312..1080674) Hypothetical protein YGR295C 0 1081590 1082735 ON 7 COS6 complement(1081590..1082735) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YGR296W 1 1084870 1090597 ON 7 YRF1-3 (1084870..1084888,1085037..1090597) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YGR296C-A 0 1088876 1089451 OFF 7 YGR296C-A complement(1088876..1089451) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL07R YGR296C-B 0 1089752 1090234 OFF 7 YGR296C-B complement(1089752..1090234) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; contained completely within TEL07R YHL050C 0 445 3310 ON 8 YHL050C complement(445..1897,2670..3310) Putative protein of unknown function, potential Cdc28p substrate YHL050W-A 1 811 1293 OFF 8 YHL050W-A (811..1293) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHL049C 0 3725 4540 ON 8 YHL049C complement(3725..4540) Putative protein of unknown function YHL048C-A 0 5662 5796 OFF 8 YHL048C-A complement(5662..5796) Putative protein of unknown function; identified by expression profiling and mass spectrometry YHL048W 1 6400 7545 ON 8 COS8 (6400..7545) Nuclear membrane protein, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; regulation suggests a potential role in the unfolded protein response YHL047C 0 8298 10211 ON 8 ARN2 complement(8298..10211) Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C YHL046W-A 1 9979 10305 OFF 8 YHL046W-A (9979..10305) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL046C 0 11921 12283 ON 8 YHL046C complement(11921..12283) Putative protein of unknown function; not an essential gene YHL045W 1 12500 12847 OFF 8 YHL045W (12500..12847) Putative protein of unknown function; not an essential gene YHL044W 1 13563 14270 ON 8 YHL044W (13563..14270) Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern YHL043W 1 14899 15411 ON 8 ECM34 (14899..15411) Putative protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YHL042W 1 15665 16117 ON 8 YHL042W (15665..16117) Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YHL041W 1 17390 17839 OFF 8 YHL041W (17390..17839) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YHL040C 0 19085 20968 ON 8 ARN1 complement(19085..20968) Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores YHL039W 1 21780 23537 ON 8 YHL039W (21780..23537) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YHL038C 0 23614 25506 ON 8 CBP2 complement(23614..25506) Mitochondrial protein required for splicing of the group I intron aI5 of the COB pre-mRNA, binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene YHL037C 0 25698 26177 OFF 8 YHL037C complement(25698..26177) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL036W 1 26239 27879 ON 8 MUP3 (26239..27879) Low affinity methionine permease, similar to Mup1p YHL035C 0 27976 32754 ON 8 VMR1 complement(27976..32754) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ATP-binding cassette (ABC) family; potential Cdc28p substrate; detected in purified mitochondria in high-throughput studies YHL034W-A 1 33174 33635 OFF 8 YHL034W-A (33174..33635) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL034C 0 33191 34075 ON 8 SBP1 complement(33191..34075) Nucleolar single-strand nucleic acid binding protein; associates with small nuclear RNAs YHL033C 0 35253 36023 ON 8 RPL8A complement(35253..36023) Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits YHL032C 0 36377 38506 ON 8 GUT1 complement(36377..38506) Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p YHL031C 0 38813 39484 ON 8 GOS1 complement(38813..39484) v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 YHL030W-A 1 39072 39533 OFF 8 YHL030W-A (39072..39533) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene GOS1/YHL031C YHL030W 1 40082 45688 ON 8 ECM29 (40082..45688) Major component of the proteasome; tethers the proteasome core particle to the regulatory particle, and enhances the stability of the proteasome YHL029C 0 45927 47966 ON 8 YHL029C complement(45927..47966) Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YHL028W 1 48761 50578 ON 8 WSC4 (48761..50578) ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 YHL027W 1 51109 52986 ON 8 RIM101 (51109..52986) Transcriptional repressor involved in response to pH and in cell wall construction; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by proteolytic processing; similar to A. nidulans PacC YHL026C 0 53217 54164 ON 8 YHL026C complement(53217..54164) Putative protein of unknown function; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) YHL025W 1 54849 55847 ON 8 SNF6 (54849..55847) Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p YHL024W 1 56647 58788 ON 8 RIM4 (56647..58788) Putative RNA-binding protein required for the expression of early and middle sporulation genes YHL023C 0 59121 62561 ON 8 RMD11 complement(59121..62561) Protein required for sporulation YHL022C 0 62959 64155 ON 8 SPO11 complement(62959..64155) Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation YHL021C 0 64459 65856 ON 8 YHL021C complement(64459..65856) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHL020C 0 66239 67453 ON 8 OPI1 complement(66239..67453) Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance YHL019W-A 1 67708 68301 OFF 8 YHL019W-A (67708..68301) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene APM2/YHL019C YHL019C 0 67728 69545 ON 8 APM2 complement(67728..69545) Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport YHL018W 1 69705 70067 ON 8 YHL018W (69705..70067) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS YHL017W 1 70273 71871 ON 8 YHL017W (70273..71871) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles YHL016C 0 72034 74241 ON 8 DUR3 complement(72034..74241) Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway YHL015W-A 1 74696 74779 OFF 8 YHL015W-A (74696..74779) Putative protein of unknown function YHL015W 1 75409 75774 ON 8 RPS20 (75409..75774) Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins YHL014C 0 76094 77311 ON 8 YLF2 complement(76094..77311) Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHL013C 0 77427 78350 ON 8 OTU2 complement(77427..78350) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization YHL012W 1 78932 80413 ON 8 YHL012W (78932..80413) Putative protein of unknown function, has some homology to Ugp1p, which encodes UDP-glucose pyrophosphorylase YHL011C 0 80650 81612 ON 8 PRS3 complement(80650..81612) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YHL010C 0 81960 83717 ON 8 YHL010C complement(81960..83717) Putative protein of unknown function, contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences YHL009C 0 84064 85056 ON 8 YAP3 complement(84064..85056) Basic leucine zipper (bZIP) transcription factor YHL008C 0 92623 94506 ON 8 YHL008C complement(92623..94506) Putative protein of unknown function, does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YHL007C 0 95114 97933 ON 8 STE20 complement(95114..97933) Signal transducing kinase of the PAK (p21-activated kinase) family, involved in pheromone response and pseudohyphal/invasive growth pathways, activated by Cdc42p; binds Ste4p at a GBB motif present in noncatalytic domains of PAK kinases YHL006W-A 1 97728 98081 OFF 8 YHL006W-A (97728..98081) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL006C 0 98339 98791 ON 8 SHU1 complement(98339..98791) Protein of unassigned function involved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation; associates with Shu2p, Psy3p, and Csm2p YHL005C 0 98824 99216 OFF 8 YHL005C complement(98824..99216) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W YHL004W 1 99215 100399 ON 8 MRP4 (99215..100399) Mitochondrial ribosomal protein of the small subunit YHL003C 0 100644 101879 ON 8 LAG1 complement(100644..101879) Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p YHL002C-A 0 102582 103070 OFF 8 YHL002C-A complement(102582..103070) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHL002W 1 102607 103965 ON 8 HSE1 (102607..103965) Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes YHL001W 1 104272 105086 ON 8 RPL14B (104272..104400,104799..105086) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similarity to rat L14 ribosomal protein YHR001W 1 106050 107363 ON 8 OSH7 (106050..107363) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YHR001W-A 1 107821 108117 ON 8 QCR10 (107821..107826,107890..108117) Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain YHR002W 1 108807 109880 ON 8 LEU5 (108807..109880) Mitochondrial carrier protein involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein; does not encode an isozyme of Leu4p, as first hypothesized YHR003C 0 110023 111312 ON 8 YHR003C complement(110023..111312) Protein of unknown function, localized to the mitochondrial outer membrane YHR004C 0 111749 113089 ON 8 NEM1 complement(111749..113089) Catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic genes; required for normal nuclear envelope morphology and sporulation YHR005C 0 113494 114912 ON 8 GPA1 complement(113494..114912) GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome YHR005C-A 0 115615 115896 ON 8 MRS11 complement(115615..115896) Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that mediates insertion of hydrophobic proteins at the inner membrane, has homology to Mrs5p, which is also involved in this process YHR006W 1 117809 119434 ON 8 STP2 (117809..119434) Transcription factor, activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes YHR007C 0 120086 121678 ON 8 ERG11 complement(120086..121678) Lanosterol 14-alpha-demethylase, catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family YHR007C-A 0 122545 122760 OFF 8 YHR007C-A complement(122545..122760) Putative protein of unknown function; identified by expression profiling and mass spectrometry YHR008C 0 122884 123585 ON 8 SOD2 complement(122884..123585) Mitochondrial superoxide dismutase; protects cells against oxygen toxicity YHR009C 0 124104 125675 ON 8 YHR009C complement(124104..125675) Putative protein of unknown function; not an essential gene YHR010W 1 126515 127486 ON 8 RPL27A (126515..126545,127107..127486) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similarity to rat L27 ribosomal protein YHR011W 1 127774 129114 ON 8 DIA4 (127774..129114) Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth YHR012W 1 129475 130442 ON 8 VPS29 (129475..129522,129642..130442) Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval YHR013C 0 130724 131440 ON 8 ARD1 complement(130724..131440) Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing YHR014W 1 132040 132915 ON 8 SPO13 (132040..132915) Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II YHR015W 1 134547 136526 ON 8 MIP6 (134547..136526) Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export YHR016C 0 136874 138448 ON 8 YSC84 complement(136874..138233,138402..138448) Protein involved in the organization of the actin cytoskeleton; contains SH3 domain similar to Rvs167p YHR017W 1 138687 139844 ON 8 YSC83 (138687..139844) Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 YHR018C 0 140004 141395 ON 8 ARG4 complement(140004..141395) Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway YHR019C 0 141887 143551 ON 8 DED81 complement(141887..143551) Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA YHR020W 1 143989 146055 ON 8 YHR020W (143989..146055) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene YHR021C 0 147864 148662 ON 8 RPS27B complement(147864..148109,148660..148662) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Ap and has similarity to rat S27 ribosomal protein YHR021W-A 1 149218 149673 OFF 8 ECM12 (149218..149673) Non-essential protein of unknown function YHR022C 0 149568 150338 ON 8 YHR022C complement(149568..150338) Putative protein of unknown function; YHR022C is not an essential gene. YHR022C-A 0 151210 151299 OFF 8 YHR022C-A complement(151210..151299) Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR023W 1 151659 157445 ON 8 MYO1 (151659..157445) Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively YHR024C 0 157737 159185 ON 8 MAS2 complement(157737..159185) Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins YHR025W 1 159431 160504 ON 8 THR1 (159431..160504) Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway YHR026W 1 160837 161478 ON 8 PPA1 (160837..161478) Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain YHR027C 0 161723 164704 ON 8 RPN1 complement(161723..164704) Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions YHR028C 0 164971 167427 ON 8 DAP2 complement(164971..167427) Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p YHR028W-A 1 167345 167665 OFF 8 YHR028W-A (167345..167665) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR029C 0 167670 168554 ON 8 YHI9 complement(167670..168554) Protein of unknown function that is a member of the PhzF superfamily, although unlike its bacterial homolog, is most likely not involved in phenazine production; possibly involved in a membrane regulation metabolic pathway YHR030C 0 168883 170337 ON 8 SLT2 complement(168883..170337) Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway YHR031C 0 170792 172963 ON 8 RRM3 complement(170792..172963) DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p YHR032W 1 173337 175082 ON 8 YHR032W (173337..175082) Putative protein of unknown function; putative substrate of the cAMP-dependent protein kinase (PKA) YHR032W-A 1 175188 175367 OFF 8 YHR032W-A (175188..175367) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032C-A YHR032C-A 0 175268 175387 OFF 8 YHR032C-A complement(175268..175387) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YHR032W-A YHR033W 1 175541 176812 ON 8 YHR033W (175541..176812) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YHR034C 0 176958 177992 ON 8 PIH1 complement(176958..177992) Protein of unresolved function; may function in protein folding and/or rRNA processing, interacts with a chaperone (Hsp82p), two chromatin remodeling factors (Rvb1p, Rvb2p) and two rRNA processing factors (Rrp43p, Nop58p) YHR035W 1 178212 180104 ON 8 YHR035W (178212..180104) Putative protein of unknown function; not an essential gene YHR036W 1 180338 181753 ON 8 BRL1 (180338..181753) Essential nuclear envelope integral membrane protein identified as a suppressor of a cold-sensitive mutant of CRM1, a karyopherin; homologous to and interacts with Brr6p, which is a nuclear envelope protein involved in nuclear export YHR037W 1 181970 183697 ON 8 PUT2 (181970..183697) Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism YHR038W 1 184059 184751 ON 8 RRF1 (184059..184751) Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria YHR039C 0 184868 186802 ON 8 MSC7 complement(184868..186802) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids YHR039C-A 0 187166 187672 ON 8 VMA10 complement(187166..187507,187670..187672) Vacuolar H+ ATPase subunit G of the catalytic (V1) sector, involved in vacuolar acidification YHR040W 1 187917 189017 ON 8 BCD1 (187917..189017) Essential protein required for the accumulation of box C/D snoRNA YHR041C 0 189124 189857 ON 8 SRB2 complement(189124..189742,189844..189857) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance YHR042W 1 190536 192611 ON 8 NCP1 (190536..192611) NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p YHR043C 0 192798 193538 ON 8 DOG2 complement(192798..193538) 2-deoxyglucose-6-phosphate phosphatase, member of a family of low molecular weight phosphatases, similar to Dog1p, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed YHR044C 0 194061 194801 ON 8 DOG1 complement(194061..194801) 2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified YHR045W 1 195544 197226 ON 8 YHR045W (195544..197226) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YHR046C 0 197391 198278 ON 8 INM1 complement(197391..198278) Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate YHR047C 0 198733 201303 ON 8 AAP1 complement(198733..201303) Arginine/alanine aminopeptidase, overproduction stimulates glycogen accumulation YHR048W 1 204600 206144 ON 8 YHR048W (204600..206144) Putative protein of unknown function, has similarity to multidrug resistance proteins; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles YHR049W 1 206455 207186 ON 8 FSH1 (206455..207186) Serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to Fsh2p and Fsh3p YHR049C-A 0 207213 207524 OFF 8 YHR049C-A complement(207213..207524) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR050W 1 207648 209297 ON 8 SMF2 (207648..209297) Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins YHR050W-A 1 209469 209639 OFF 8 YHR050W-A (209469..209639) Protein of unknown function; identified by expression profiling and mass spectrometry YHR051W 1 209699 210145 ON 8 COX6 (209699..210145) Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels YHR052W 1 210842 211972 ON 8 CIC1 (210842..211972) Essential protein that interacts with proteasome components and has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles YHR052W-A 1 212512 212706 OFF 8 YHR052W-A (212512..212706) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CUP1-1 YHR053C 0 212537 212722 ON 8 CUP1-1 complement(212537..212722) Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C YHR054C 0 213187 214251 OFF 8 YHR054C complement(213187..214251) Putative protein of unknown function YHR054W-A 1 214510 214704 OFF 8 YHR054W-A (214510..214704) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CUP1-2 YHR055C 0 214535 214720 ON 8 CUP1-2 complement(214535..214720) Metallothionein, binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C YHR056C 0 215185 217836 ON 8 RSC30 complement(215185..217836) Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc3p; null mutants are osmosensitive YHR056W-A 1 217408 217842 OFF 8 YHR056W-A (217408..217842) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR057C 0 218228 218845 ON 8 CPR2 complement(218228..218845) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway YHR058C 0 218999 219886 ON 8 MED6 complement(218999..219886) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation YHR059W 1 220110 220502 ON 8 FYV4 (220110..220502) Protein of unknown function, required for survival upon exposure to K1 killer toxin YHR060W 1 220727 221272 ON 8 VMA22 (220727..221272) Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) YHR061C 0 221536 222480 ON 8 GIC1 complement(221536..222480) Protein of unknown function involved in initiation of budding and cellular polarization, interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain YHR062C 0 222879 223760 ON 8 RPP1 complement(222879..223760) Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends YHR063C 0 224032 225171 ON 8 PAN5 complement(224032..225171) 2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE YHR063W-A 1 225444 225779 OFF 8 YHR063W-A (225444..225779) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR064C 0 225527 227143 ON 8 SSZ1 complement(225527..227143) Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP YHR065C 0 227534 229039 ON 8 RRP3 complement(227534..229039) Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity YHR066W 1 229337 230698 ON 8 SSF1 (229337..230698) Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family YHR067W 1 230972 231814 ON 8 HTD2 (230972..231814) Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology YHR068W 1 232135 233298 ON 8 DYS1 (232135..233298) Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric YHR069C 0 233581 234660 ON 8 RRP4 complement(233581..234660) 3'-5' exoribonuclease involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp43p and Dis3p YHR069C-A 0 234876 235238 OFF 8 YHR069C-A complement(234876..235238) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR070W 1 234883 236382 ON 8 TRM5 (234883..236382) tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya YHR070C-A 0 236104 236514 OFF 8 YHR070C-A complement(236104..236514) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene TRM5/YHR070W YHR071W 1 237006 237695 ON 8 PCL5 (237006..237695) Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity YHR071C-A 0 238912 239232 OFF 8 YHR071C-A complement(238912..239232) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ERG7/YHR072W YHR072W 1 239100 241295 ON 8 ERG7 (239100..241295) Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis YHR072W-A 1 241666 241842 ON 8 NOP10 (241666..241842) Constituent of small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs, which are required for pseudouridylation and processing of pre-18S rRNA YHR073W 1 242584 245574 ON 8 OSH3 (242584..245574) Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YHR073W-A 1 242870 243046 OFF 8 YHR073W-A (242870..243046) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry YHR073C-B 0 245424 245504 OFF 8 YHR073C-B complement(245424..245504) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF YHR073W; identified by expression profiling and mass spectrometry YHR074W 1 246195 248339 ON 8 QNS1 (246195..248339) Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide YHR075C 0 248441 249643 ON 8 PPE1 complement(248441..249643) Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein YHR076W 1 251103 252227 ON 8 PTC7 (251103..251157,251251..252227) Mitochondrially localized type 2C protein phosphatase; expression induced by growth on ethanol and by sustained osmotic stress; possible role in carbon source utilization in low oxygen environments YHR077C 0 252376 255758 ON 8 NMD2 complement(252376..255639,255753..255758) Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance YHR078W 1 256362 258020 ON 8 YHR078W (256362..258020) High osmolarity-regulated gene of unknown function YHR079C 0 258246 261593 ON 8 IRE1 complement(258246..261593) Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress YHR079C-A 0 262194 262555 ON 8 SAE3 complement(262194..262356,262443..262555) Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p YHR080C 0 262803 266840 ON 8 YHR080C complement(262803..266840) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHR081W 1 267540 268094 ON 8 LRP1 (267540..268094) Substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs; required for telomere length maintenance; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination YHR082C 0 268461 271550 ON 8 KSP1 complement(268461..271550) Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation YHR083W 1 272629 273618 ON 8 SAM35 (272629..273618) Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane YHR084W 1 274176 276242 ON 8 STE12 (274176..276242) Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth YHR085W 1 276766 277770 ON 8 IPI1 (276766..277770) Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles YHR086W 1 278155 279726 ON 8 NAM8 (278155..279726) RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA YHR086W-A 1 280233 280394 OFF 8 YHR086W-A (280233..280394) Putative protein of unknown function; identified by fungal homology and RT-PCR YHR087W 1 280822 281157 ON 8 YHR087W (280822..281157) Protein of unknown function involved in RNA metabolism; this single domain protein has structural similarity to the N-terminal domain of SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1/YLR022C) YHR088W 1 281497 282384 ON 8 RPF1 (281497..282384) Nucleolar protein involved in the assembly of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA YHR089C 0 282683 283300 ON 8 GAR1 complement(282683..283300) Protein component of the H/ACA snoRNP pseudouridylase complex, involved in the modification and cleavage of the 18S pre-rRNA YHR090C 0 283779 284627 ON 8 YNG2 complement(283779..284627) Subunit of the NuA4 histone acetyltransferase complex that acetylates histone H4 and H2A; has similarity to the human tumor suppressor ING1 YHR091C 0 284841 286772 ON 8 MSR1 complement(284841..286772) Mitochondrial arginyl-tRNA synthetase YHR092C 0 287132 288814 ON 8 HXT4 complement(287132..288814) High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose YHR093W 1 289145 289693 OFF 8 AHT1 (289145..289693) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region YHR094C 0 290916 292628 ON 8 HXT1 complement(290916..292628) Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting YHR095W 1 292946 293380 OFF 8 YHR095W (292946..293380) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR096C 0 294672 296450 ON 8 HXT5 complement(294672..296450) Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs YHR097C 0 297388 298612 ON 8 YHR097C complement(297388..298363,298488..298612) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus YHR098C 0 299148 301937 ON 8 SFB3 complement(299148..301937) Member of the Sec24p family; forms a complex, with Sec23p, that is involved in sorting of Pma1p into COPII vesicles; peripheral ER membrane protein; potential Cdc28p substrate YHR099W 1 302764 313998 ON 8 TRA1 (302764..313998) Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation YHR100C 0 314119 314676 ON 8 YHR100C complement(314119..314676) Putative protein of unknown function, required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHR101C 0 314877 315971 ON 8 BIG1 complement(314877..315774,315862..315971) Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan YHR102W 1 316575 319817 ON 8 KIC1 (316575..319817) Protein kinase of the PAK/Ste20 kinase family, required for cell integrity possibly through regulating 1,6-beta-glucan levels in the wall; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body YHR103W 1 320417 322975 ON 8 SBE22 (320417..322975) Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth YHR104W 1 323412 324395 ON 8 GRE3 (323412..324395) Aldose reductase involved in methylglyoxal, d-xylose and arabinose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway YHR105W 1 324769 325413 ON 8 YPT35 (324769..325413) Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport YHR106W 1 325601 326629 ON 8 TRR2 (325601..326629) Mitochondrial thioredoxin reductase involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p, contains active-site motif (CAVC) present in prokaryotic thioredoxin reductases, binds NADPH and FAD YHR107C 0 326816 328039 ON 8 CDC12 complement(326816..328039) Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM YHR108W 1 328306 330063 ON 8 GGA2 (328306..330063) Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi YHR109W 1 330313 332070 ON 8 CTM1 (330313..332070) Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth YHR110W 1 332285 332923 ON 8 ERP5 (332285..332923) Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport YHR111W 1 333075 334397 ON 8 UBA4 (333075..334397) Protein that activates Urm1p before its conjugation to proteins (urmylation); one target is the thioredoxin peroxidase Ahp1p, suggesting a role of urmylation in the oxidative stress response YHR112C 0 334530 335666 ON 8 YHR112C complement(334530..335666) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YHR113W 1 336340 337812 ON 8 YHR113W (336340..337812) Cytoplasmic aspartyl aminopeptidase; cleaves unblocked N-terminal acidic amino acid residues from peptide substrates; forms a 12 subunit homo-oligomeric complex; M18 metalloprotease family member; may interact with ribosomes YHR114W 1 338086 339987 ON 8 BZZ1 (338086..339987) SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins YHR115C 0 340112 341362 ON 8 DMA1 complement(340112..341362) Protein involved in regulating spindle position and orientation, functionally redundant with Dma2p; homolog of S. pombe Dma1 and H. sapiens Chfr YHR116W 1 341668 342123 ON 8 COX23 (341668..342123) Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p YHR117W 1 342352 344271 ON 8 TOM71 (342352..344271) Mitochondrial outer membrane protein with similarity to Tom70p; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins YHR118C 0 344324 345631 ON 8 ORC6 complement(344324..345631) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p YHR119W 1 346046 349288 ON 8 SET1 (346046..349288) Histone methyltransferase, subunit of the COMPASS (Set1C) complex which methylates histone H3 on lysine 4; required in transcriptional silencing near telomeres and at the silent mating type loci; contains a SET domain YHR120W 1 349577 352456 ON 8 MSH1 (349577..352456) DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis YHR121W 1 352759 353322 ON 8 LSM12 (352759..353322) Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS YHR122W 1 353628 354323 ON 8 YHR122W (353628..354323) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; may be involved in transcription; YHR122W is an essential gene YHR123W 1 354818 356084 ON 8 EPT1 (354818..354867,354959..356084) sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability YHR124W 1 356564 358447 ON 8 NDT80 (356564..358447) Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) YHR125W 1 358861 359166 OFF 8 YHR125W (358861..359166) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR126C 0 359705 360184 ON 8 YHR126C complement(359705..360184) Putative protein of unknown function; transcription dependent upon Azf1p YHR127W 1 360916 361647 ON 8 YHR127W (360916..361647) Protein of unknown function; localizes to the nucleus YHR128W 1 362118 362768 ON 8 FUR1 (362118..362768) Uracil phosphoribosyltransferase, synthesizes UMP from uracil; involved in the pyrimidine salvage pathway YHR129C 0 363002 364156 ON 8 ARP1 complement(363002..364156) Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin YHR130C 0 364968 365303 OFF 8 YHR130C complement(364968..365303) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR131C 0 365343 367895 ON 8 YHR131C complement(365343..367895) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YHR131W-A 1 367750 367995 OFF 8 YHR131W-A (367750..367995) Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YHR131C YHR132C 0 368505 369797 ON 8 ECM14 complement(368505..369797) Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly YHR132W-A 1 370057 370452 ON 8 YHR132W-A (370057..370452) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YHR133C 0 370725 371600 ON 8 NSG1 complement(370725..371600) Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins YHR134W 1 371752 372561 ON 8 WSS1 (371752..372561) Sumoylated protein of unknown function, identified based on genetic interactions with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response, processing stalled or collapsed replication forks YHR135C 0 372697 374313 ON 8 YCK1 complement(372697..374313) Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p YHR136C 0 374657 375103 ON 8 SPL2 complement(374657..375103) Protein with similarity to cyclin-dependent kinase inhibitors, overproduction suppresses a plc1 null mutation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YHR137W 1 375712 377253 ON 8 ARO9 (375712..377253) Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism YHR137C-A 0 375741 376391 OFF 8 YHR137C-A complement(375741..376391) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene ARO9/YHR137W; identified by gene-trapping, expression analysis, and genome-wide homology searching YHR138C 0 377358 377702 ON 8 YHR138C complement(377358..377702) Putative protein of unknown function; has similarity to Pbi2p; double null mutant lacking Pbi2p and Yhr138p exhibits highly fragmented vacuoles YHR139C 0 378222 379202 ON 8 SPS100 complement(378222..379202) Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall YHR139C-A 0 380111 380422 OFF 8 YHR139C-A complement(380111..380422) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR140W 1 380575 381294 ON 8 YHR140W (380575..381294) Putative integral membrane protein of unknown function YHR141C 0 381993 382754 ON 8 RPL42B complement(381993..382309,382751..382754) Protein component of the large (60S) ribosomal subunit, identical to Rpl42Ap and has similarity to rat L44; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus YHR142W 1 383541 384491 ON 8 CHS7 (383541..384491) Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER YHR143W 1 385513 386490 ON 8 DSE2 (385513..386490) Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP YHR143W-A 1 387236 387448 ON 8 RPC10 (387236..387448) RNA polymerase subunit, found in RNA polymerase complexes I, II, and III YHR144C 0 387791 388729 ON 8 DCD1 complement(387791..388729) Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT cell cycle regulated YHR145C 0 388984 389340 OFF 8 YHR145C complement(388984..389340) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR146W 1 390303 391700 ON 8 CRP1 (390303..391700) Protein that binds to cruciform DNA structures YHR147C 0 392642 393286 ON 8 MRPL6 complement(392642..393286) Mitochondrial ribosomal protein of the large subunit YHR148W 1 393537 394088 ON 8 IMP3 (393537..394088) Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA YHR149C 0 394458 396662 ON 8 SKG6 complement(394458..396662) Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p YHR150W 1 397254 398993 ON 8 PEX28 (397254..398993) Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p YHR151C 0 399271 400851 ON 8 YHR151C complement(399271..400851) Putative protein of unknown function YHR152W 1 401437 401958 ON 8 SPO12 (401437..401958) Nucleolar protein of unknown function, positive regulator of exit from mitosis; involved in regulating the release of Cdc14p from the nucleolus in early anaphase; proposed to play similar role in meiosis YHR153C 0 402089 402685 ON 8 SPO16 complement(402089..402685) Protein of unknown function, required for spore formation YHR154W 1 402969 406181 ON 8 RTT107 (402969..406181) Protein implicated in Mms22-dependent DNA repair during S phase, DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition YHR155W 1 407106 410792 ON 8 YSP1 (407106..410792) Mitochondrial protein with a potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone YHR156C 0 411387 412409 ON 8 LIN1 complement(411387..412409) Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication YHR157W 1 412910 413458 ON 8 REC104 (412910..413458) Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination YHR158C 0 413688 417182 ON 8 KEL1 complement(413688..417182) Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate YHR159W 1 417552 419066 ON 8 YHR159W (417552..419066) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate YHR160C 0 419224 420075 ON 8 PEX18 complement(419224..420075) Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p YHR161C 0 420376 422289 ON 8 YAP1801 complement(420376..422289) Protein involved in clathrin cage assembly; binds Pan1p and clathrin; homologous to Yap1802p, member of the AP180 protein family YHR162W 1 423075 423464 ON 8 YHR162W (423075..423464) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YHR163W 1 423726 424475 ON 8 SOL3 (423726..424475) 6-phosphogluconolactonase, catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p YHR164C 0 424612 429180 ON 8 DNA2 complement(424612..429180) Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate YHR165C 0 429709 436950 ON 8 PRP8 complement(429709..436950) Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing YHR165W-A 1 436649 436960 OFF 8 YHR165W-A (436649..436960) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR166C 0 437172 439052 ON 8 CDC23 complement(437172..439052) Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YHR167W 1 439344 440129 ON 8 THP2 (439344..440129) Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance YHR168W 1 440379 441878 ON 8 MTG2 (440379..441878) Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly YHR169W 1 442182 443477 ON 8 DBP8 (442182..443477) Putative ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 40S ribosomal subunit YHR170W 1 443829 445385 ON 8 NMD3 (443829..445385) Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex YHR171W 1 445713 447605 ON 8 ATG7 (445713..447605) Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation YHR172W 1 448335 450806 ON 8 SPC97 (448335..450806) Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque YHR173C 0 450815 451153 OFF 8 YHR173C complement(450815..451153) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR174W 1 451327 452640 ON 8 ENO2 (451327..452640) Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose YHR175W 1 452872 453441 ON 8 CTR2 (452872..453441) Putative low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation YHR175W-A 1 453558 453707 OFF 8 YHR175W-A (453558..453707) Putative protein of unknown function; identified by fungal homology and RT-PCR YHR176W 1 454229 455527 ON 8 FMO1 (454229..455527) Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins YHR177W 1 456591 457952 ON 8 YHR177W (456591..457952) Putative protein of unknown function YHR178W 1 459299 461530 ON 8 STB5 (459299..461530) Activator of multidrug resistance genes, forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a PDRE (pleotropic drug resistance element) in vitro; binds Sin3p in a two-hybrid assay YHR179W 1 462502 463704 ON 8 OYE2 (462502..463704) Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism YHR180W 1 465178 465669 OFF 8 YHR180W (465178..465669) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YHR180W-A 1 466932 467114 OFF 8 YHR180W-A (466932..467114) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious ORF YHR180C-B and long terminal repeat YHRCsigma3 YHR180C-B 0 466958 467062 OFF 8 YHR180C-B complement(466958..467062) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YHR180W-A YHR181W 1 467228 467914 ON 8 SVP26 (467228..467914) Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment YHR182W 1 468219 470576 ON 8 YHR182W (468219..470576) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm YHR182C-A 0 470939 471412 OFF 8 YHR182C-A complement(470939..471412) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR183W 1 470960 472429 ON 8 GND1 (470960..472429) 6-phosphogluconate dehydrogenase (decarboxylating), catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress YHR184W 1 472744 474459 ON 8 SSP1 (472744..474459) Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis YHR185C 0 474627 475340 ON 8 PFS1 complement(474627..475340) Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies of a cell during meiosis II; not required for meiotic recombination or meiotic chromosome segregation YHR186C 0 475999 480672 ON 8 KOG1 complement(475999..480672) Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors YHR187W 1 480990 481919 ON 8 IKI1 (480990..481919) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin YHR188C 0 482005 483837 ON 8 GPI16 complement(482005..483837) Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog YHR189W 1 484028 484600 ON 8 PTH1 (484028..484600) One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth and for mitochondrial respiration YHR190W 1 484845 486179 ON 8 ERG9 (484845..486179) Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway YHR191C 0 486230 486631 ON 8 CTF8 complement(486230..486631) Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion YHR192W 1 486826 487662 ON 8 YHR192W (486826..487662) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS YHR193C 0 487712 488236 ON 8 EGD2 complement(487712..488236) Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes YHR193C-A 0 488653 489027 OFF 8 YHR193C-A complement(488653..489027) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified ORF MDM31 YHR194W 1 488657 490396 ON 8 MDM31 (488657..490396) Mitochondrial inner membrane protein with similarity to Mdm32p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 YHR195W 1 490747 491712 ON 8 NVJ1 (490747..491712) Nuclear envelope protein that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) YHR196W 1 491931 493658 ON 8 UTP9 (491931..493658) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YHR197W 1 493896 496187 ON 8 RIX1 (493896..496187) Essential component of the Rix1 complex (Rix1p, Ipi1p, Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles YHR198C 0 496315 497280 ON 8 YHR198C complement(496315..497280) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHR199C 0 497490 498422 ON 8 YHR199C complement(497490..498422) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YHR199C-A 0 498571 498859 ON 8 YHR199C-A complement(498571..498719,498787..498859) Putative protein of unknown function; transcribed sequence appears to contain an intron YHR200W 1 499079 499885 ON 8 RPN10 (499079..499885) Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein YHR201C 0 499950 501143 ON 8 PPX1 complement(499950..501143) Exopolyphosphatase, hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix YHR202W 1 502388 504196 ON 8 YHR202W (502388..504196) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization YHR203C 0 504476 505530 ON 8 RPS4B complement(504476..505247,505517..505530) Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to rat S4 ribosomal protein YHR204W 1 506319 508709 ON 8 MNL1 (506319..508709) Alpha mannosidase-like protein of the endoplasmic reticulum required for degradation of glycoproteins but not for processing of N-linked oligosaccharides YHR205W 1 509363 511837 ON 8 SCH9 (509363..511837) Protein kinase that regulates signal transduction activity and G1 progression, controls cAPK activity, required for nitrogen activation of the FGM pathway, involved in life span regulation, homologous to mammalian Akt/PKB YHR206W 1 512732 514600 ON 8 SKN7 (512732..514600) Nuclear response regulator and transcription factor, part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation YHR207C 0 514905 516485 ON 8 SET5 complement(514905..516485) Zinc-finger protein of unknown function, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus YHR208W 1 517532 518713 ON 8 BAT1 (517532..518713) Mitochondrial branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase YHR209W 1 519437 520312 ON 8 YHR209W (519437..520312) Putative S-adenosylmethionine-dependent methyltransferase; mediates cantharidin resistance YHR210C 0 520712 521737 ON 8 YHR210C complement(520712..521737) Putative protein of unknown function; non-essential gene; highly expressed under anaeorbic conditions; sequence similarity to aldose 1-epimerases such as GAL10 YHR211W 1 525392 528619 ON 8 FLO5 (525392..528619) Lectin-like cell wall protein (flocculin) involved in flocculation, binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; similar to Flo1p YHR212C 0 537759 538094 OFF 8 YHR212C complement(537759..538094) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR212W-A 1 538742 538945 OFF 8 YHR212W-A (538742..538945) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR213W 1 539151 539747 ON 8 YHR213W (539151..539747) Putative protein of unknown function YHR213W-A 1 540549 540782 OFF 8 YHR213W-A (540549..540782) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR213W-B 1 540800 541099 OFF 8 YHR213W-B (540800..541099) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR214W 1 541651 542262 ON 8 YHR214W (541651..542262) Putative protein of unknown function; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein YHR214W-A 1 543008 543493 OFF 8 YHR214W-A (543008..543493) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; similar to the fungal specific protein encoded by YAR068W; induced by zinc deficiency YHR214C-D 0 550648 550941 OFF 8 YHR214C-D complement(550648..550941) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR214C-E 0 551200 551499 OFF 8 YHR214C-E complement(551200..551499) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YHR215W 1 552099 553502 ON 8 PHO12 (552099..553502) One of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface by the secretory pathway; nearly identical to Pho11p; upregulated by phosphate starvation YHR216W 1 554396 555967 ON 8 IMD2 (554396..555967) Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation YHR217C 0 556581 557042 OFF 8 YHR217C complement(556581..557042) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; located in the telomeric region TEL08R. YHR218W 1 558014 559924 ON 8 YHR218W (558014..558615,558715..559924) Helicase-like protein encoded within the telomeric Y' element YHR218W-A 1 559932 560249 OFF 8 YHR218W-A (559932..560249) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YHR219W 1 560173 562047 ON 8 YHR219W (560173..562047) Putative protein of unknown function with similarity to helicases; located in the telomere region on the right arm of chromosome VIII YHR219C-A 0 561199 561681 OFF 8 YHR219C-A complement(561199..561681) Dubious open reading frame unlikely to encode a functional protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YIL177C 0 483 6147 ON 9 YIL177C complement(483..4598,4987..6147) Putative protein of unknown function; similarity to DNA helicases that are encoded within subtelomeric Y' elements and induced in telomerase deficient survivors YIL177W-A 1 846 1328 OFF 9 YIL177W-A (846..1328) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YIL177C YIL176C 0 8793 9155 ON 9 YIL176C complement(8793..9155) Hypothetical protein YIL175W 1 9183 9500 OFF 9 YIL175W (9183..9500) Hypothetical protein YIL174W 1 9469 9696 OFF 9 YIL174W (9469..9696) Hypothetical protein YIL173W 1 11492 16141 ON 9 VTH1 (11492..16141) Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting YIL172C 0 16784 18553 ON 9 YIL172C complement(16784..18553) Putative protein of unknown function with similarity to glucosidases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YIL171W-A 1 18260 18712 OFF 9 YIL171W-A (18260..18712) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL171W 1 19515 19844 ON 9 YIL171W (19515..19844) Possible pseudogene in strain S288C; YIL171W and the adjacent ORF, YIL170W/HXT12, together encode a non-functional member of the hexose transporter family YIL170W 1 19847 21220 ON 9 HXT12 (19847..21220) Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family YIL169C 0 23119 26106 ON 9 YIL169C complement(23119..26106) Putative protein of unknown function; serine/threonine rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; YIL169C is a non-essential gene YIL168W 1 29032 29415 ON 9 YIL168W (29032..29415) Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase YIL167W 1 29416 30048 ON 9 SDL1 (29416..30048) Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase YIL166C 0 30938 32566 ON 9 YIL166C complement(30938..32566) Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene YIL165C 0 33718 34077 ON 9 YIL165C complement(33718..34077) Putative protein of unknown function; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene YIL164C 0 34087 34686 ON 9 NIT1 complement(34087..34686) Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene YIL163C 0 36899 37252 OFF 9 YIL163C complement(36899..37252) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL162W 1 37385 38983 ON 9 SUC2 (37385..38983) Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively YIL161W 1 39433 40140 ON 9 YIL161W (39433..40140) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene YIL160C 0 40191 41444 ON 9 POT1 complement(40191..41444) 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids YIL159W 1 41825 45952 ON 9 BNR1 (41825..45952) Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 YIL158W 1 46201 46815 ON 9 YIL158W (46201..46815) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YIL157C 0 46949 47542 ON 9 YIL157C complement(46949..47542) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant is defective in cytochrome oxidase and is respiratory deficient YIL156W-A 1 47292 47693 OFF 9 YIL156W-A (47292..47693) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL156W-B 1 47690 47973 ON 9 YIL156W-B (47690..47698,47761..47973) Putative protein of unknown function, originally identified based on homology to Ashbya gossypii and other related yeasts YIL156W 1 48091 51306 ON 9 UBP7 (48091..51306) Ubiquitin-specific protease that cleaves ubiquitin-protein fusions YIL155C 0 51759 53708 ON 9 GUT2 complement(51759..53708) Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner YIL154C 0 53981 55021 ON 9 IMP2' complement(53981..55021) Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat YIL153W 1 55198 56379 ON 9 RRD1 (55198..56379) Activator of the phosphotyrosyl phosphatase activity of PP2A, peptidyl-prolyl cis/trans-isomerase; regulates G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; subunit of the Tap42p-Sit4p-Rrd1p complex YIL152W 1 56545 57252 ON 9 YIL152W (56545..57252) Putative protein of unknown function YIL151C 0 57338 60694 ON 9 YIL151C complement(57338..60694) Putative protein of unknown function, predicted to contain a PINc (PilT N terminus) domain YIL150C 0 61013 62728 ON 9 MCM10 complement(61013..62728) Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins YIL149C 0 63028 68067 ON 9 MLP2 complement(63028..68067) Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length YIL148W 1 68708 69528 ON 9 RPL40A (68708..68715,69150..69528) Fusion protein, identical to Rpl40Bp, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes YIL147C 0 69791 73453 ON 9 SLN1 complement(69791..73453) Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators YIL146C 0 74184 75773 ON 9 ECM37 complement(74184..75773) Non-essential protein of unknown function YIL145C 0 76354 77283 ON 9 PAN6 complement(76354..77283) Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC YIL144W 1 78074 80149 ON 9 TID3 (78074..80149) Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering YIL143C 0 80510 83041 ON 9 SSL2 complement(80510..83041) Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3 YIL142C-A 0 83208 83540 OFF 9 YIL142C-A complement(83208..83540) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL142W 1 83302 84885 ON 9 CCT2 (83302..84885) Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YIL141W 1 85053 85442 OFF 9 YIL141W (85053..85442) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL140W 1 85366 87837 ON 9 AXL2 (85366..87837) Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate YIL139C 0 87979 88716 ON 9 REV7 complement(87979..88716) Processivity subunit of DNA polymerase zeta, which is involved in DNA repair and translesion synthesis; required for mutagenesis induced by DNA damage YIL138C 0 89230 89715 ON 9 TPM2 complement(89230..89715) Minor isoform of tropomyosin, binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p YIL137C 0 89948 92788 ON 9 TMA108 complement(89948..92788) Protein that associates with ribosomes; putative metalloprotease YIL136W 1 93619 94800 ON 9 OM45 (93619..94800) Protein of unknown function, major constituent of the mitochondrial outer membrane; located on the outer (cytosolic) face of the outer membrane YIL135C 0 95065 96375 ON 9 VHS2 complement(95065..96375) Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p YIL134C-A 0 96522 96725 OFF 9 YIL134C-A complement(96522..96725) Putative protein of unknown function, identified by fungal homology and RT-PCR YIL134W 1 97395 98330 ON 9 FLX1 (97395..98330) Protein required for transport of flavin adenine dinucleotide (FAD), a synthesis product of riboflavin, across the mitochondrial membrane YIL133C 0 98527 99416 ON 9 RPL16A complement(98527..99095,99386..99416) N-terminally acetylated protein component of the large (60S) ribosomal subunit, binds to 5.8 S rRNA; has similarity to Rpl16Bp, E. coli L13 and rat L13a ribosomal proteins; transcriptionally regulated by Rap1p YIL132C 0 99860 100501 ON 9 CSM2 complement(99860..100501) Protein required for accurate chromosome segregation during meiosis YIL131C 0 100781 102235 ON 9 FKH1 complement(100781..102235) Forkhead family transcription factor with a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; regulates donor preference during switching YIL130W 1 102782 105676 ON 9 ASG1 (102782..105676) Proposed transcriptional activator, member of the Gal4p family of zinc cluster proteins YIL129C 0 106107 113237 ON 9 TAO3 complement(106107..113237) Protein involved in cell morphogenesis and proliferation, associated with protein kinase Cbk1p; mutants activate OCH1 transcription YIL128W 1 113806 116904 ON 9 MET18 (113806..116904) DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; involved in telomere maintenance YIL127C 0 117024 117644 ON 9 YIL127C complement(117024..117644) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus YIL126W 1 117992 122071 ON 9 STH1 (117992..122071) ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p YIL125W 1 122689 125733 ON 9 KGD1 (122689..125733) Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA YIL124W 1 126204 127097 ON 9 AYR1 (126204..127097) NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones YIL123W 1 128151 129578 ON 9 SIM1 (128151..129578) Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated YIL122W 1 130607 131662 ON 9 POG1 (130607..131662) Putative transcriptional activator that promotes recovery from pheromone induced arrest; inhibits both alpha-factor induced G1 arrest and repression of CLN1 and CLN2 via SCB/MCB promoter elements; potential Cdc28p substrate; SBF regulated YIL121W 1 132241 133869 ON 9 QDR2 (132241..133869) Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, barban, cisplatin, and bleomycin; may have a role in potassium uptake YIL120W 1 134414 136105 ON 9 QDR1 (134414..136105) Multidrug transporter of the major facilitator superfamily, required for resistance to quinidine, ketoconazole, fluconazole, and barba YIL119C 0 136651 137874 ON 9 RPI1 complement(136651..137874) Putative transcriptional regulator; overexpression suppresses the heat shock sensitivity of wild-type RAS2 overexpression and also suppresses the cell lysis defect of an mpk1 mutation YIL118W 1 139749 140444 ON 9 RHO3 (139749..140444) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p YIL117C 0 140610 141566 ON 9 PRM5 complement(140610..141566) Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling YIL116W 1 142925 144082 ON 9 HIS5 (142925..144082) Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts YIL115W-A 1 144265 144636 OFF 9 YIL115W-A (144265..144636) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene NUP159/YIL115C YIL115C 0 144324 148706 ON 9 NUP159 complement(144324..148706) Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, forms a subcomplex with Nup82p and Nsp1p, required for mRNA export YIL114C 0 149140 149985 ON 9 POR2 complement(149140..149985) Putative mitochondrial porin (voltage-dependent anion channel), related to Por1p but not required for mitochondrial membrane permeability or mitochondrial osmotic stability YIL113W 1 150556 151185 ON 9 SDP1 (150556..151185) Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock YIL112W 1 151592 154843 ON 9 HOS4 (151592..154843) Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate YIL111W 1 155219 155762 ON 9 COX5B (155219..155219,155308..155762) Subunit Vb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth YIL110W 1 156042 157175 ON 9 YIL110W (156042..157175) Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; deletion mutant exhibits a weak vacuolar protein sorting defect, enhanced resistance to caspofungin, and is synthetically lethal with MEN mutants YIL109C 0 157382 160162 ON 9 SEC24 complement(157382..160162) Component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat; involved in ER to Golgi transport, cargo selection and autophagy; required for the binding of the Sec13 complex to ER membranes; homologous to Lst1p and Lss1p YIL108W 1 160884 162974 ON 9 YIL108W (160884..162974) Putative metalloprotease YIL107C 0 163275 165758 ON 9 PFK26 complement(163275..165758) 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, has negligible fructose-2,6-bisphosphatase activity, transcriptional regulation involves protein kinase A YIL106W 1 166412 167441 ON 9 MOB1 (166412..166431,166517..167441) Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex YIL105C 0 167578 169638 ON 9 SLM1 complement(167578..169638) Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex YIL105W-A 1 167661 167801 OFF 9 YIL105W-A (167661..167801) Dubious open reading frame; completely overlaps the verified gene SLM1; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YIL104C 0 169979 171502 ON 9 SHQ1 complement(169979..171502) Essential nuclear protein, required for accumulation of box H/ACA snoRNAs and for rRNA processing; interacts with Naf1p YIL103W 1 171748 173025 ON 9 DPH1 (171748..173025) Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p YIL102C-A 0 173362 173589 ON 9 YIL102C-A complement(173362..173589) Putative protein of unknown function, identified based on comparisons of the genome sequences of six Saccharomyces species YIL102C 0 174579 174884 ON 9 YIL102C complement(174579..174884) Putative protein of unknown function YIL101C 0 175304 177247 ON 9 XBP1 complement(175304..177247) Transcriptional repressor that binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate YIL100C-A 0 177340 177678 OFF 9 YIL100C-A complement(177340..177678) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL100W 1 177370 177723 OFF 9 YIL100W (177370..177723) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A YIL099W 1 178001 179650 ON 9 SGA1 (178001..179650) Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation YIL098C 0 179769 180236 ON 9 FMC1 complement(179769..180236) Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p YIL097W 1 180424 181974 ON 9 FYV10 (180424..181974) Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains CTLH domain YIL096C 0 182114 183124 ON 9 YIL096C complement(182114..183124) Putative protein of unknown function; associates with precursors of the 60S ribosomal subunit YIL095W 1 183934 186366 ON 9 PRK1 (183934..186366) Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton through the phosphorylation of the Pan1p-Sla1p-End3p protein complex YIL094C 0 186514 187629 ON 9 LYS12 complement(186514..187629) Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate YIL093C 0 187987 188781 ON 9 RSM25 complement(187987..188781) Mitochondrial ribosomal protein of the small subunit YIL092W 1 189063 190964 ON 9 YIL092W (189063..190964) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus YIL091C 0 191027 193192 ON 9 YIL091C complement(191027..193192) Protein required for cell viability YIL090W 1 193592 195067 ON 9 ICE2 (193592..195067) Integral ER membrane protein with type-III transmembrane domains; mutations cause defects in cortical ER morphology in both the mother and daughter cells YIL089W 1 195596 196213 ON 9 YIL089W (195596..196213) Putative protein of unknown function YIL088C 0 197928 199400 ON 9 AVT7 complement(197928..199400) Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YIL087C 0 199643 200116 ON 9 YIL087C complement(199643..200116) Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YIL086C 0 200150 200458 OFF 9 YIL086C complement(200150..200458) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL085C 0 200487 202040 ON 9 KTR7 complement(200487..202040) Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YIL084C 0 202273 203256 ON 9 SDS3 complement(202273..203256) Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes YIL083C 0 203553 204650 ON 9 YIL083C complement(203553..204650) Putative phosphopantothenoylcysteine synthetase (PPCS) that catalyzes the second step in the biosynthesis of coenzyme A from pantothenate; conserved in bacteria, humans, and plants; essential for viability YIL080W 1 205632 210354 OFF 9 YIL080W (205632..206481,206483..210354) TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition YIL082W 1 205632 206504 OFF 9 YIL082W (205632..206504) Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag YIL079C 0 210920 212002 ON 9 AIR1 complement(210920..212002) RING finger protein that interacts with the arginine methyltransferase Hmt1p to regulate methylation of Npl3p, which modulates Npl3p function in mRNA processing and export; has similarity to Air2p YIL078W 1 212496 214700 ON 9 THS1 (212496..214700) Threonyl-tRNA synthetase, essential cytoplasmic protein YIL077C 0 214988 215950 ON 9 YIL077C complement(214988..215950) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL076W 1 216655 217545 ON 9 SEC28 (216655..217545) Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth YIL075C 0 217860 220697 ON 9 RPN2 complement(217860..220697) Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p YIL074C 0 221078 222487 ON 9 SER33 complement(221078..222487) 3-phosphoglycerate dehydrogenase, catalyzes the first step in serine and glycine biosynthesis; isozyme of Ser3p YIL073C 0 222934 225951 ON 9 SPO22 complement(222934..225806,225897..225951) Meiosis-specific protein essential for chromosome synapsis, similar to phospholipase A2, involved in completion of nuclear divisions during meiosis; induced early in meiosis YIL072W 1 226599 228416 ON 9 HOP1 (226599..228416) Meiosis-specific DNA binding protein that displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for homologous chromosome synapsis and chiasma formation YIL071W-A 1 228547 229023 OFF 9 YIL071W-A (228547..229023) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL071C 0 228657 229991 ON 9 PCI8 complement(228657..229991) Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain YIL070C 0 230269 231069 ON 9 MAM33 complement(230269..231069) Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R YIL069C 0 231550 232366 ON 9 RPS24B complement(231550..231954,232364..232366) Protein component of the small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein YIL068W-A 1 233010 233393 OFF 9 YIL068W-A (233010..233393) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL068C 0 233054 235471 ON 9 SEC6 complement(233054..235471) Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesicles to active sites of exocytosis; dimeric form of Sec6p interacts with Sec9p in vitro and inhibits t-SNARE assembly YIL067C 0 235721 237757 ON 9 YIL067C complement(235721..237757) Uncharacterized protein of unknown function YIL066W-A 1 237363 237806 OFF 9 YIL066W-A (237363..237806) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL066C 0 238096 240705 ON 9 RNR3 complement(238096..240705) Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits YIL065C 0 241305 241772 ON 9 FIS1 complement(241305..241772) Mitochondrial outer membrane protein involved in membrane fission, required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation YIL064W 1 241940 242713 ON 9 YIL064W (241940..242713) Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family YIL063C 0 242758 243741 ON 9 YRB2 complement(242758..243741) Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential YIL062C 0 243995 244459 ON 9 ARC15 complement(243995..244459) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YIL061C 0 244654 245556 ON 9 SNP1 complement(244654..245556) Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein YIL060W 1 246389 246823 OFF 9 YIL060W (246389..246823) Putative protein of unknown function; mutant accumulates less glycogen than does wild type; YIL060W is not an essential gene YIL059C 0 246547 246912 OFF 9 YIL059C complement(246547..246912) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W YIL058W 1 246911 247195 OFF 9 YIL058W (246911..247195) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL057C 0 247899 248393 ON 9 YIL057C complement(247899..248393) Putative protein of unknown function; expression induced under carbon limitation and repressed under high glucose YIL056W 1 249989 251911 ON 9 VHR1 (249989..251911) Transcriptional activator, required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations YIL055C 0 252040 253923 ON 9 YIL055C complement(252040..253923) Putative protein of unknown function YIL054W 1 254541 254858 OFF 9 YIL054W (254541..254858) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL053W 1 255113 255865 ON 9 RHR2 (255113..255865) Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress YIL052C 0 256224 257061 ON 9 RPL34B complement(256224..256552,257025..257061) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl34Ap and has similarity to rat L34 ribosomal protein YIL051C 0 257842 258279 ON 9 MMF1 complement(257842..258279) Mitochondrial protein involved in maintenance of the mitochondrial genome YIL050W 1 258912 259769 ON 9 PCL7 (258912..259769) Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated YIL049W 1 260157 260918 ON 9 DFG10 (260157..260918) Protein of unknown function, involved in filamentous growth YIL048W 1 261436 264891 ON 9 NEO1 (261436..264891) Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus YIL047C-A 0 265098 265466 OFF 9 YIL047C-A complement(265098..265466) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL047C 0 265114 267822 ON 9 SYG1 complement(265114..267822) Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency YIL046W-A 1 268308 268472 OFF 9 YIL046W-A (268308..268472) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIL046W 1 268650 270572 ON 9 MET30 (268650..270572) F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus YIL045W 1 271160 272776 ON 9 PIG2 (271160..272776) Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase YIL044C 0 272949 273845 ON 9 AGE2 complement(272949..273845) ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif YIL043C 0 274071 274925 ON 9 CBR1 complement(274071..274925) Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia YIL042C 0 275107 276291 ON 9 PKP1 complement(275107..276291) Mitochondrial kinase, phosphorylates pyruvate dehydrogenase alpha subunit Pda1p YIL041W 1 276524 277504 ON 9 GVP36 (276524..277504) Golgi vesicle protein of unknown function; localizes to both early and late Golgi vesicles; may interact with ribosomes, based on co-purification experiments YIL040W 1 277722 278138 ON 9 APQ12 (277722..278138) Protein involved in nucleocytoplasmic transport of mRNA YIL039W 1 278425 279846 ON 9 YIL039W (278425..279846) Putative protein of unknown function; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL038C 0 280141 282651 ON 9 NOT3 complement(280141..282651) Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation YIL037C 0 283028 284998 ON 9 PRM2 complement(283028..284998) Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p YIL036W 1 285665 287428 ON 9 CST6 (285665..287428) Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, activates transcription of genes involved in utilization of non-optimal carbon sources; involved in telomere maintenance YIL035C 0 287789 288907 ON 9 CKA1 complement(287789..288907) Alpha catalytic subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA2, CKB1 and CKB2, the many substrates include transcription factors and all RNA polymerases YIL034C 0 289225 290088 ON 9 CAP2 complement(289225..290088) Beta subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches YIL033C 0 290418 291668 ON 9 BCY1 complement(290418..291668) Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation YIL032C 0 291960 292316 OFF 9 YIL032C complement(291960..292316) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL031W 1 292632 295736 ON 9 ULP2 (292632..295736) Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate YIL030W-A 1 295564 295902 OFF 9 YIL030W-A (295564..295902) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIL031W. YIL030C 0 296049 300008 ON 9 SSM4 complement(296049..300008) Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation YIL029W-A 1 299734 300105 OFF 9 YIL029W-A (299734..300105) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL029C 0 300827 301255 ON 9 YIL029C complement(300827..301255) Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YIL028W 1 302098 302496 OFF 9 YIL028W (302098..302496) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL027C 0 303677 304102 ON 9 KRE27 complement(303677..304102) Protein of unknown function; null mutant shows K1 killer toxin resistance YIL026C 0 304475 307927 ON 9 IRR1 complement(304475..307927) Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability YIL025C 0 308199 308573 OFF 9 YIL025C complement(308199..308573) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL024C 0 308600 309169 ON 9 YIL024C complement(308600..309169) Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p YIL023C 0 309384 310424 ON 9 YKE4 complement(309384..310424) Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family YIL022W 1 311163 312458 ON 9 TIM44 (311163..312458) Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane YIL021W 1 312903 313859 ON 9 RPB3 (312903..313859) RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit YIL021C-A 0 313197 313466 OFF 9 YIL021C-A complement(313197..313466) Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene RPB3/YIL021W; identified by gene-trapping, expression analysis, and genome-wide homology searching YIL020C-A 0 313711 314049 OFF 9 YIL020C-A complement(313711..314049) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIL020C 0 314033 314818 ON 9 HIS6 complement(314033..314818) Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts YIL019W 1 315091 316131 ON 9 FAF1 (315091..316131) Protein required for pre-rRNA processing and 40S ribosomal subunit assembly YIL018W 1 316766 317930 ON 9 RPL2B (316766..316769,317170..317930) Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures YIL017C 0 318198 320963 ON 9 VID28 complement(318198..320963) Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm YIL016W 1 321452 321931 ON 9 SNL1 (321452..321931) Protein of unknown function proposed to be involved in nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein YIL015W 1 322340 324103 ON 9 BAR1 (322340..324103) Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest YIL014C-A 0 325210 325524 ON 9 YIL014C-A complement(325210..325524) Putative protein of unknown function YIL014W 1 326101 327993 ON 9 MNT3 (326101..327993) Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation YIL013C 0 328205 332440 ON 9 PDR11 complement(328205..332440) ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromisedregulated by Pdr1p; required for anaerobic growth YIL012W 1 333009 333401 OFF 9 YIL012W (333009..333401) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YIL011W 1 333724 334533 ON 9 TIR3 (333724..334533) Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth YIL010W 1 334879 335526 ON 9 DOT5 (334879..335526) Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth YIL009C-A 0 335663 336209 ON 9 EST3 complement(335663..335932,335934..336209) Component of the telomerase holoenzyme, involved in telomere replication YIL009W 1 339341 341425 ON 9 FAA3 (339341..341425) Long chain fatty acyl-CoA synthetase, has a preference for C16 and C18 fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery YIL008W 1 342533 342832 ON 9 URM1 (342533..342832) Ubiquitin-like protein with only weak sequence similarity to ubiquitin; depends on the E1-like activating enzyme Uba4p; molecular function of the Urm1p pathway is unknown, but it is required for normal growth, particularly at high temperature YIL007C 0 342991 343653 ON 9 NAS2 complement(342991..343653) Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p YIL006W 1 344059 345180 ON 9 YIA6 (344059..345180) Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs YIL005W 1 345689 347794 ON 9 EPS1 (345689..347794) Pdi1p (protein disulfide isomerase)-related protein involved in endoplasmic reticulum retention of resident ER proteins YIL004C 0 347943 348502 ON 9 BET1 complement(347943..348360,348492..348502) Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins YIL003W 1 349119 350000 ON 9 CFD1 (349119..350000) Highly conserved, putative P-loop ATPase localized in the cytoplasm; has a potential role in assembly of iron-sulfur clusters in proteins YIL002W-A 1 350298 350507 ON 9 YIL002W-A (350298..350507) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIL002C 0 350588 353428 ON 9 INP51 complement(350588..353428) Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth YIL001W 1 353937 355478 ON 9 YIL001W (353937..355478) Hypothetical protein YIR001C 0 356140 356892 ON 9 SGN1 complement(356140..356892) Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation YIR002C 0 357412 360393 ON 9 MPH1 complement(357412..360393) Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype YIR003W 1 360882 362921 ON 9 YIR003W (360882..362921) Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein colocalizes with Sac1p to the actin cytoskeleton; interacts with with the SH3 domain of Abp1p YIR004W 1 363218 364516 ON 9 DJP1 (363218..364516) Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ YIR005W 1 364886 365332 ON 9 IST3 (364886..365332) Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing YIR006C 0 365463 369905 ON 9 PAN1 complement(365463..369905) Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease YIR007W 1 370701 372995 ON 9 YIR007W (370701..372995) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene YIR008C 0 373074 374303 ON 9 PRI1 complement(373074..374303) Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair YIR009W 1 374522 374857 ON 9 MSL1 (374522..374857) U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro; does not contain the conserved C-terminal RNA binding domain found in other family members YIR010W 1 375428 377158 ON 9 DSN1 (375428..377158) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation YIR011C 0 377284 378243 ON 9 STS1 complement(377284..378243) Protein that interacts with the karyopherin Srp1p; may have a role with Srp1p in ubiquitin-mediated protein degradation YIR012W 1 378483 379778 ON 9 SQT1 (378483..379778) Essential protein involved in a late step of 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay YIR013C 0 380016 380381 ON 9 GAT4 complement(380016..380381) Protein containing GATA family zinc finger motifs YIR014W 1 381083 381811 ON 9 YIR014W (381083..381811) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene YIR015W 1 381945 382379 ON 9 RPR2 (381945..382379) Subunit of nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits YIR016W 1 382625 383422 ON 9 YIR016W (382625..383422) Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR016W is a non-essential gene YIR017C 0 383553 384116 ON 9 MET28 complement(383553..384116) Transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism YIR017W-A 1 383563 384162 OFF 9 YIR017W-A (383563..384162) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR018W 1 384606 385343 ON 9 YAP5 (384606..385343) Basic leucine zipper (bZIP) transcription factor YIR018C-A 0 385561 385698 OFF 9 YIR018C-A complement(385561..385698) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIR019C 0 389569 393672 ON 9 MUC1 complement(389569..393672) GPI-anchored cell surface glycoprotein (flocculin) required for diploid pseudohyphal formation and haploid invasive growth, transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p) YIR020C 0 394252 394554 OFF 9 YIR020C complement(394252..394554) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR020W-A 1 394914 395156 OFF 9 YIR020W-A (394914..395156) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YIR020C-B 0 397212 397946 OFF 9 YIR020C-B complement(397212..397946) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF MRS1 YIR021W 1 397291 398382 ON 9 MRS1 (397291..398382) Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA YIR021W-A 1 398511 398723 OFF 9 YIR021W-A (398511..398723) Putative protein of unknown function; identified by expression profiling and mass spectrometry YIR022W 1 398730 399233 ON 9 SEC11 (398730..399233) 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum YIR023W 1 399774 402686 ON 9 DAL81 (399774..402686) Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism YIR023C-A 0 402380 402703 OFF 9 YIR023C-A complement(402380..402703) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR024C 0 402838 403488 ON 9 YIR024C complement(402838..403488) Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect YIR025W 1 403656 404762 ON 9 MND2 (403656..404762) Subunit of the anaphase-promoting complex (APC); needed for meiotic nuclear division YIR026C 0 404870 405964 ON 9 YVH1 complement(404870..405964) Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; transcription induced by low temperature and nitrogen starvation; member of the dual-specificity family of protein phosphatases YIR027C 0 406257 407639 ON 9 DAL1 complement(406257..407639) Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression YIR028W 1 408465 410372 ON 9 DAL4 (408465..410372) Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR029W 1 410804 411835 ON 9 DAL2 (410804..411835) Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR030C 0 412033 412767 ON 9 DCG1 complement(412033..412767) Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain YIR030W-A 1 412896 413279 OFF 9 YIR030W-A (412896..413279) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR031C 0 413012 414676 ON 9 DAL7 complement(413012..414676) Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation YIR032C 0 415027 415614 ON 9 DAL3 complement(415027..415614) Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression YIR033W 1 416121 419462 ON 9 MGA2 (416121..419462) ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting YIR034C 0 419612 420733 ON 9 LYS1 complement(419612..420733) Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway YIR035C 0 421023 421787 ON 9 YIR035C complement(421023..421787) Putative cytoplasmic protein of unknown function YIR036C 0 422071 422862 ON 9 YIR036C complement(422071..422862) Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52 foci YIR036W-A 1 422632 423033 OFF 9 YIR036W-A (422632..423033) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YIR037W 1 423125 423616 ON 9 HYR1 (423125..423616) Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor YIR038C 0 423806 424510 ON 9 GTT1 complement(423806..424510) ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p YIR039C 0 430494 432107 ON 9 YPS6 complement(430494..432107) Putative GPI-anchored aspartic protease YIR040C 0 433385 433717 OFF 9 YIR040C complement(433385..433717) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YIR041W 1 433925 434299 ON 9 PAU15 (433925..434299) Hypothetical protein YIR042C 0 435270 435980 ON 9 YIR042C complement(435270..435980) Putative protein of unknown function; YIR042C is a non-essential gene YIR043C 0 437040 437732 OFF 9 YIR043C complement(437040..437732) Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family YIR044C 0 437991 438176 ON 9 YIR044C complement(437991..438176) Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family YJL225C 0 466 6130 ON 10 YJL225C complement(466..4581,4970..6130) Putative protein of unknown function; similarity to DNA helicases that are also encoded within subtelomeric Y' elements and induced in telomerase deficient survivors YJL225W-A 1 829 1311 OFF 10 YJL225W-A (829..1311) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized gene YJL225C YJL223C 0 8776 9138 ON 10 PAU1 complement(8776..9138) Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YJL222W-B 1 9346 9483 OFF 10 YJL222W-B (9346..9483) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL222W-A 1 9452 9679 OFF 10 YJL222W-A (9452..9679) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL222W 1 11475 16124 ON 10 VTH2 (11475..16124) Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting YJL221C 0 16767 18536 ON 10 FSP2 complement(16767..18536) Protein of unknown function, expression is induced during nitrogen limitation YJL220W 1 18243 18695 OFF 10 YJL220W (18243..18695) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL221C/FSP2 YJL219W 1 19497 21200 ON 10 HXT9 (19497..21200) Putative hexose transporter that is nearly identical to Hxt11p, has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p YJL218W 1 21973 22563 ON 10 YJL218W (21973..22563) Putative protein of unknown function, similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene YJL217W 1 23133 23729 ON 10 YJL217W (23133..23729) Cytoplasmic protein of unknown function; expression induced by calcium shortage and via the copper sensing transciption factor Mac1p during conditons of copper deficiency; mRNA is cell cycle regulated, peaking in G1 phase YJL216C 0 24341 26086 ON 10 YJL216C complement(24341..26086) Protein of unknown function, similar to alpha-D-glucosidases; transcriptionally activated by both Pdr8p and Yrm1p, along with transporters and other genes involved in the pleiotropic drug resistance (PDR) phenomenon YJL215C 0 26412 26771 OFF 10 YJL215C complement(26412..26771) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL214W 1 26887 28596 ON 10 HXT8 (26887..28596) Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose YJL213W 1 32163 33158 ON 10 YJL213W (32163..33158) Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p YJL212C 0 33850 36249 ON 10 OPT1 complement(33850..36249) Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family YJL211C 0 36757 37200 OFF 10 YJL211C complement(36757..37200) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 YJL210W 1 36919 37734 ON 10 PEX2 (36919..37734) RING-finger peroxin, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import YJL209W 1 38005 39969 ON 10 CBP1 (38005..39969) Mitochondrial protein that interacts with the 5'-untranslated region of the COB mRNA and has a role in its stability and translation; found in a complex at the inner membrane along with Pet309p YJL208C 0 40194 41183 ON 10 NUC1 complement(40194..41183) Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy YJL207C 0 41389 47433 ON 10 LAA1 complement(41389..47433) AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene YJL206C 0 47659 49935 ON 10 YJL206C complement(47659..49935) Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment YJL205C 0 50139 50443 ON 10 NCE101 complement(50139..50268,50412..50443) Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences YJL204C 0 50629 53151 ON 10 RCY1 complement(50629..53151) F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth YJL203W 1 53341 54183 ON 10 PRP21 (53341..54183) Subunit of the SF3a splicing factor complex, required for spliceosome assembly YJL202C 0 53943 54290 OFF 10 YJL202C complement(53943..54290) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex YJL201W 1 54379 56178 ON 10 ECM25 (54379..56178) Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p YJL200C 0 56444 58813 ON 10 ACO2 complement(56444..58813) Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol YJL199C 0 59855 60181 OFF 10 MBB1 complement(59855..60181) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies YJL198W 1 60843 63488 ON 10 PHO90 (60843..63488) Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth YJL197W 1 63804 67568 ON 10 UBP12 (63804..67568) Ubiquitin carboxyl-terminal hydrolase, ubiquitin-specific protease present in the nucleus and cytoplasm that cleaves ubiquitin from ubiquitinated proteins YJL197C-A 0 65804 66085 OFF 10 YJL197C-A complement(65804..66085) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL197C/UBP12 YJL196C 0 67849 68781 ON 10 ELO1 complement(67849..68781) Elongase I, medium-chain acyl elongase, catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids YJL195C 0 69240 69941 OFF 10 YJL195C complement(69240..69941) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 YJL194W 1 69337 70878 ON 10 CDC6 (69337..70878) Essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p YJL193W 1 71365 72573 ON 10 YJL193W (71365..72573) Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect YJL192C 0 72709 73413 ON 10 SOP4 complement(72709..73413) ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER YJL191W 1 73786 74610 ON 10 RPS14B (73786..73795,74204..74610) Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins YJL190C 0 74909 75301 ON 10 RPS22A complement(74909..75301) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Bp and has similarity to E. coli S8 and rat S15a ribosomal proteins YJL189W 1 75932 76473 ON 10 RPL39 (75932..75937,76324..76473) Protein component of the large (60S) ribosomal subunit, has similarity to rat L39 ribosomal protein; required for ribosome biogenesis; exhibits genetic interactions with SIS1 and PAB1 YJL188C 0 76201 76509 OFF 10 BUD19 complement(76201..76509) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay YJL187C 0 76802 79261 ON 10 SWE1 complement(76802..79261) Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate YJL186W 1 80153 81913 ON 10 MNN5 (80153..81913) Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment YJL185C 0 82093 82974 ON 10 YJL185C complement(82093..82974) Putative protein of unknown function; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene YJL184W 1 83446 83817 ON 10 GON7 (83446..83817) Protein proposed to be involved in the modification of N-linked oligosaccharides, osmotic stress response, telomere uncapping and elongation, transcription; component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Bud32p YJL183W 1 84066 85334 ON 10 MNN11 (84066..85334) Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p YJL182C 0 85433 85750 OFF 10 YJL182C complement(85433..85750) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W YJL181W 1 85658 87493 ON 10 YJL181W (85658..87493) Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis YJL180C 0 87581 88558 ON 10 ATP12 complement(87581..88558) Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency YJL179W 1 88785 89114 ON 10 PFD1 (88785..89114) Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin YJL178C 0 89280 90095 ON 10 ATG27 complement(89280..90095) Type II membrane protein involved in autophagy; binds phosphatidylinositol 3-phosphate, required for the cytoplasm-to-vacuole targeting (Cvt) pathway YJL177W 1 90784 91655 ON 10 RPL17B (90784..91092,91410..91655) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similarity to E. coli L22 and rat L17 ribosomal proteins YJL176C 0 92051 94528 ON 10 SWI3 complement(92051..94528) Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2 YJL175W 1 94047 94559 OFF 10 YJL175W (94047..94559) Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor YJL174W 1 95090 95920 ON 10 KRE9 (95090..95920) Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects YJL173C 0 96159 96527 ON 10 RFA3 complement(96159..96527) Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination YJL172W 1 97731 99461 ON 10 CPS1 (97731..99461) Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions YJL171C 0 99698 100888 ON 10 YJL171C complement(99698..100888) GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein YJL170C 0 101144 101773 ON 10 ASG7 complement(101144..101773) Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor YJL169W 1 102093 102461 OFF 10 YJL169W (102093..102461) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 YJL168C 0 102221 104422 ON 10 SET2 complement(102221..104422) Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p YJL167W 1 105008 106066 ON 10 ERG20 (105008..106066) Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis YJL166W 1 106428 106712 ON 10 QCR8 (106428..106712) Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p YJL165C 0 106888 109455 ON 10 HAL5 complement(106888..109455) Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters YJL164C 0 109960 111153 ON 10 TPK1 complement(109960..111153) cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk2p and Tpk3p YJL163C 0 111662 113329 ON 10 YJL163C complement(111662..113329) Putative protein of unknown function YJL162C 0 113874 115625 ON 10 JJJ2 complement(113874..115625) Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein YJL161W 1 117241 117783 ON 10 YJL161W (117241..117783) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL160C 0 117958 118821 ON 10 YJL160C complement(117958..118821) Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis YJL159W 1 120445 121686 ON 10 HSP150 (120445..121686) O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation YJL158C 0 122261 122944 ON 10 CIS3 complement(122261..122944) Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family YJL157C 0 123832 126324 ON 10 FAR1 complement(123832..126324) Cyclin-dependent kinase inhibitor that mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate YJL156W-A 1 126600 126821 OFF 10 YJL156W-A (126600..126821) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL156C 0 126886 128949 ON 10 SSY5 complement(126886..128949) Probable serine protease of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that regulate expression of amino acid permease genes YJL155C 0 129282 130640 ON 10 FBP26 complement(129282..130640) Fructose-2,6-bisphosphatase, required for glucose metabolism YJL154C 0 131098 133932 ON 10 VPS35 complement(131098..133932) Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval YJL153C 0 134329 135930 ON 10 INO1 complement(134329..135930) Inositol 1-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element YJL152W 1 136171 136530 OFF 10 YJL152W (136171..136530) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL151C 0 136369 136770 ON 10 SNA3 complement(136369..136770) Integral membrane protein localized to vacuolar intralumenal vesicles, computational analysis of large-scale protein-protein interaction data suggests a possible role in either cell wall synthesis or protein-vacuolar targeting YJL150W 1 137120 137422 OFF 10 YJL150W (137120..137422) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL149W 1 137376 139367 ON 10 YJL149W (137376..139367) Putative SCF ubiquitin ligase F-box protein of unknown function; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C; YJL149W is a non-essential gene YJL148W 1 140434 141135 ON 10 RPA34 (140434..141135) RNA polymerase I subunit A34.5 YJL147C 0 141416 142564 ON 10 YJL147C complement(141416..142564) Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene YJL146W 1 143289 144698 ON 10 IDS2 (143289..144698) Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation YJL145W 1 145157 146041 ON 10 SFH5 (145157..146041) Putative phosphatidylinositol transfer protein (PITP), exhibits phosphatidylinositol- but not phosphatidylcholine-transfer activity, mainly localized to cytosol and microsomes, similar to Sec14p; may be PITP regulator rather than actual PITP YJL144W 1 146356 146670 ON 10 YJL144W (146356..146670) Cytoplasmic hydrophilin of unknown function, possibly involved in the dessication response; expression induced by osmotic stress, starvation and during stationary phase; GFP-fusion protein is induced by the DNA-damaging agent MMS YJL143W 1 147098 147574 ON 10 TIM17 (147098..147574) Essential constituent of the mitochondrial inner membrane presequence translocase; interacts with Pam18p to recruit the presequence translocase-associated motor (PAM complex) and also required for protein sorting during import YJL142C 0 147816 148208 OFF 10 YJL142C complement(147816..148208) Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci YJL141C 0 147964 150387 ON 10 YAK1 complement(147964..150387) Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal YJL140W 1 150958 151623 ON 10 RPB4 (150958..151623) RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; dispensable under some environmental conditions; involved in export of mRNA to cytoplasm under stress conditions; involved in telomere maintenance YJL139C 0 151710 152996 ON 10 YUR1 complement(151710..152996) Mannosyltransferase of the KTR1 family, involved in protein N-glycosylation; located in the Golgi apparatus YJL138C 0 153501 154688 ON 10 TIF2 complement(153501..154688) Translation initiation factor eIF4A, identical to Tif1p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G YJL137C 0 154982 156124 ON 10 GLG2 complement(154982..156124) Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin YJL136W-A 1 156166 156249 OFF 10 YJL136W-A (156166..156249) Identified by SAGE YJL136C 0 156547 157270 ON 10 RPS21B complement(156547..156786,157247..157270) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Ap and has similarity to rat S21 ribosomal protein YJL135W 1 157874 158191 OFF 10 YJL135W (157874..158191) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 YJL134W 1 158185 159414 ON 10 LCB3 (158185..159414) Long-chain base-1-phosphate phosphatase with specificity for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids YJL133C-A 0 159620 159844 ON 10 YJL133C-A complement(159620..159844) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL133W 1 160616 161560 ON 10 MRS3 (160616..161560) Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs4p; functions under low-iron conditions; may transport other cations in addition to iron YJL132W 1 161911 164163 ON 10 YJL132W (161911..164163) Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene YJL131C 0 164275 165345 ON 10 YJL131C complement(164275..165345) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YJL131C is not an essential gene YJL130C 0 165720 172364 ON 10 URA2 complement(165720..172364) Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP YJL129C 0 173596 177303 ON 10 TRK1 complement(173596..177303) Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation YJL128C 0 178094 180100 ON 10 PBS2 complement(178094..180100) MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress YJL127W-A 1 180191 180307 OFF 10 YJL127W-A (180191..180307) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YJL127C-B 0 181549 181707 ON 10 YJL127C-B complement(181549..181707) Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii YJL127C 0 182296 184218 ON 10 SPT10 complement(182296..184218) Putative histone acetylase, sequence-specific activator of histone genes, binds specifically and highly cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box YJL126W 1 184499 185422 ON 10 NIT2 (184499..185422) Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member YJL125C 0 185526 186677 ON 10 GCD14 complement(185526..186677) Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression YJL124C 0 187125 187643 ON 10 LSM1 complement(187125..187643) Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs YJL123C 0 188003 189439 ON 10 YJL123C complement(188003..189439) Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); YJL123C is a non-essential gene YJL122W 1 189715 190242 ON 10 ALB1 (189715..190242) Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p YJL121C 0 190373 191089 ON 10 RPE1 complement(190373..191089) D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress YJL120W 1 191021 191344 OFF 10 YJL120W (191021..191344) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO YJL119C 0 191571 191894 OFF 10 YJL119C complement(191571..191894) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL118W 1 191638 192297 ON 10 YJL118W (191638..192297) Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein YJL117W 1 192530 193465 ON 10 PHO86 (192530..193465) Endoplasmic reticulum (ER) resident protein required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles YJL116C 0 193859 194872 ON 10 NCA3 complement(193859..194872) Protein that functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p ) of the Fo-F1 ATP synthase; member of the SUN family YJL115W 1 196285 197124 ON 10 ASF1 (196285..197124) Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed YJL112W 1 205301 207445 ON 10 MDV1 (205301..207445) Peripheral protein of the cytosolic face of the mitochondrial outer membrane, required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats YJL111W 1 207873 209525 ON 10 CCT7 (207873..209525) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJL110C 0 209918 211573 ON 10 GZF3 complement(209918..211573) GATA zinc finger protein and Dal80p homolog that negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p YJL109C 0 211996 217305 ON 10 UTP10 complement(211996..217305) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YJL108C 0 217701 218852 ON 10 PRM10 complement(217701..218852) Pheromone-regulated protein, predicted to have 5 transmembrane segments YJL107C 0 218849 220012 OFF 10 YJL107C complement(218849..220012) Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi YJL106W 1 221386 223323 ON 10 IME2 (221386..223323) Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p YJL105W 1 225051 226733 ON 10 SET4 (225051..226733) Protein of unknown function, contains a SET domain YJL104W 1 227323 227772 ON 10 PAM16 (227323..227772) Constituent of the mitochondrial import motor associated with the presequence translocase, along with Ssc1p, Tim44p, Mge1p, and Pam18p; forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains J-like domain YJL103C 0 229021 230877 ON 10 YJL103C complement(229021..230877) Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis YJL102W 1 231297 233756 ON 10 MEF2 (231297..233756) Mitochondrial elongation factor involved in translational elongation YJL101C 0 234316 236352 ON 10 GSH1 complement(234316..236352) Gamma glutamylcysteine synthetase, catalyzes the first step in the gamma-glutamyl cycle for glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury YJL100W 1 237259 239082 ON 10 LSB6 (237259..239082) Phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization YJL099W 1 239410 241650 ON 10 CHS6 (239410..241650) Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane YJL098W 1 242078 245254 ON 10 SAP185 (242078..245254) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p YJL097W 1 245587 246240 ON 10 PHS1 (245587..246240) Protein of unknown function; homolog of mammalian PTPLA; involved in sphingolipid biosynthesis, protein trafficking; required for cell viability YJL096W 1 246486 246971 ON 10 MRPL49 (246486..246971) Mitochondrial ribosomal protein of the large subunit YJL095W 1 247250 251686 ON 10 BCK1 (247250..251686) Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p YJL094C 0 251816 254437 ON 10 KHA1 complement(251816..254437) Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies YJL093C 0 254732 256807 ON 10 TOK1 complement(254732..256807) Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin YJL092W 1 257418 260942 ON 10 SRS2 (257418..260942) DNA helicase and DNA-dependent ATPase involved in DNA repair, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; affects genome stability by suppressing unscheduled homologous recombination YJL091C 0 261075 262547 ON 10 GWT1 complement(261075..262547) Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors YJL090C 0 262752 265046 ON 10 DPB11 complement(262752..265046) Subunit of DNA Polymerase II Epsilon complex; has BRCT domain, required on the prereplicative complex at replication origins for loading DNA polymerases to initiate DNA synthesis, also required for S/M checkpoint control YJL089W 1 265921 268410 ON 10 SIP4 (265921..268410) C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus YJL088W 1 268794 269810 ON 10 ARG3 (268794..269810) Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine YJL087C 0 269997 272480 ON 10 TRL1 complement(269997..272480) tRNA ligase, required for tRNA splicing; composed of three essential domains containing the phosphodiesterase, polynucleotide kinase, and ligase activities required for ligation; localized at the inner membrane of the nuclear envelope YJL086C 0 272473 272841 OFF 10 YJL086C complement(272473..272841) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 YJL085W 1 272822 274693 ON 10 EXO70 (272822..274693) Essential 70kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YJL084C 0 274857 277997 ON 10 YJL084C complement(274857..277997) Cytoplasmic protein of unknown function that interacts with the cyclin Pcl7p; phosphorylated in vitro by the cyclin-CDK complex, Pcl7p-Pho85p; identified as a potential Cdc28p substrate; mRNA is cell cycle regulated, peaking in M phase YJL083W 1 278836 280650 ON 10 TAX4 (278836..280650) Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p YJL082W 1 281180 283375 ON 10 IML2 (281180..283375) Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL081C 0 283797 285266 ON 10 ARP4 complement(283797..285266) Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes YJL080C 0 285553 289221 ON 10 SCP160 complement(285553..289221) Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins YJL079C 0 289870 290769 ON 10 PRY1 complement(289870..290769) Protein of unknown function, has similarity to Pry2p and Pry3p and to the plant PR-1 class of pathogen related proteins YJL078C 0 291331 293976 ON 10 PRY3 complement(291331..293976) Protein of unknown function, has similarity to Pry1p and Pry2p and to the plant PR-1 class of pathogen related proteins YJL077W-B 1 294042 294140 OFF 10 YJL077W-B (294042..294140) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YJL077C 0 294661 295056 OFF 10 ICS3 complement(294661..295056) Protein of unknown function YJL077W-A 1 294794 294880 OFF 10 YJL077W-A (294794..294880) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL077C/ICS3 YJL076W 1 295240 298809 ON 10 NET1 (295240..298809) Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure YJL075C 0 298455 298871 OFF 10 APQ13 complement(298455..298871) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate YJL074C 0 299152 302844 ON 10 SMC3 complement(299152..302844) Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member YJL073W 1 303176 305113 ON 10 JEM1 (303176..305113) DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 YJL072C 0 305216 305857 ON 10 PSF2 complement(305216..305857) Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery YJL071W 1 306127 307851 ON 10 ARG2 (306127..307851) Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p YJL070C 0 307966 310632 ON 10 YJL070C complement(307966..310632) Putative protein of unknown function with similarity to AMP deaminases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YJL070C is a non-essential gene YJL069C 0 310917 312701 ON 10 UTP18 complement(310917..312701) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YJL068C 0 313011 313910 ON 10 YJL068C complement(313011..313910) Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D YJL067W 1 314079 314429 OFF 10 YJL067W (314079..314429) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL066C 0 314109 314867 ON 10 MPM1 complement(314109..314867) Mitochondrial membrane protein of unknown function, contains no hydrophobic stretches YJL065C 0 315049 315552 ON 10 DLS1 complement(315049..315552) Subunit of ISW2/yCHRAC chromatin accessibility complex along with Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing YJL064W 1 315170 315565 OFF 10 YJL064W (315170..315565) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 YJL063C 0 315754 316470 ON 10 MRPL8 complement(315754..316470) Mitochondrial ribosomal protein of the large subunit YJL062W-A 1 316718 316975 ON 10 YJL062W-A (316718..316975) Putative protein of unknown function, identified based on comparison to related yeast species; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL062W 1 317279 319771 ON 10 LAS21 (317279..319771) Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity YJL061W 1 320011 322152 ON 10 NUP82 (320011..322152) Nucleoporin, subunit of the nuclear pore complex (NPC); forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC YJL060W 1 323381 324715 ON 10 BNA3 (323381..324715) Arylformamidase, involved in biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; potential Cdc28p substrate YJL059W 1 324959 326185 ON 10 YHC3 (324959..326185) Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) YJL058C 0 326237 327868 ON 10 BIT61 complement(326237..327868) Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p-Slm1p-Slm2p), a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity YJL057C 0 328113 330116 ON 10 IKS1 complement(328113..330116) Putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p YJL056C 0 330426 333068 ON 10 ZAP1 complement(330426..333068) Zinc-regulated transcription factor, binds to zinc-responsive promoter elements to induce transcription of certain genes in the presence of zinc; regulates its own transcription; contains seven zinc-finger domains YJL055W 1 333352 334089 ON 10 YJL055W (333352..334089) Putative protein of unknown function; deletion mutants are sensitive to purine base analogs, 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA) and are AHA-hypermutable YJL054W 1 334260 335696 ON 10 TIM54 (334260..335696) Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane YJL053W 1 335893 337032 ON 10 PEP8 (335893..337032) Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport YJL052C-A 0 337879 337998 OFF 10 YJL052C-A complement(337879..337998) Putative protein of unknown function, identified based on comparison to related yeast species YJL052W 1 338266 339264 ON 10 TDH1 (338266..339264) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall YJL051W 1 339782 342250 ON 10 YJL051W (339782..342250) Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52 foci YJL050W 1 342517 345738 ON 10 MTR4 (342517..345738) Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA YJL049W 1 345968 347320 ON 10 YJL049W (345968..347320) Putative protein of unknown function; YJL049W is a non-essential gene YJL048C 0 347442 348632 ON 10 UBX6 complement(347442..348632) UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline YJL047C-A 0 349039 349173 OFF 10 YJL047C-A complement(349039..349173) Putative protein of unknown function YJL047C 0 349575 352103 ON 10 RTT101 complement(349575..352103) Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; deletion phenotype suggests a role in anaphase progression; interacts with Mms22p and implicated in Mms22-dependent DNA repair; modified by the ubiquitin-like protein, Rub1p YJL046W 1 352381 353610 ON 10 YJL046W (352381..353610) Putative protein of unknown function, predicted to encode a lipoate-protein ligase A family member; deletion mutant has a respiratory growth defect YJL045W 1 356019 357923 ON 10 YJL045W (356019..357923) Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner YJL044C 0 358295 359671 ON 10 GYP6 complement(358295..359671) GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport YJL043W 1 360125 360898 ON 10 YJL043W (360125..360898) Putative protein of unknown function; YJL043W is a non-essential gene YJL042W 1 361244 365440 ON 10 MHP1 (361244..365440) Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins YJL041W 1 365779 368368 ON 10 NSP1 (365779..365779,365898..368368) Essential component of the nuclear pore complex, which mediates nuclear import and export YJL039C 0 368743 373794 ON 10 NUP192 complement(368743..373794) Essential structural subunit of the nuclear pore complex (NPC), localizes to the nuclear periphery of nuclear pores, homologous to human p205 YJL038C 0 375110 375769 ON 10 YJL038C complement(375110..375769) Putative protein of unknown function; expression induced during sporulation and repressed during vegetative growth by Sum1p and Hst1p; similar to adjacent open reading frame, YJL037W YJL037W 1 376657 377331 ON 10 YJL037W (376657..377331) Putative protein of unknown function; expression induced in respiratory-deficient petite cells, in carbon-limited chemostat cultures, during meiosis, sporulation and after alpha factor treatment; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52 foci YJL036W 1 378820 380091 ON 10 SNX4 (378820..380091) Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX domain; forms complex with Snx41p and Atg20p YJL035C 0 380244 380996 ON 10 TAD2 complement(380244..380996) Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs YJL034W 1 381322 383370 ON 10 KAR2 (381322..383370) ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p YJL033W 1 383832 386144 ON 10 HCA4 (383832..386144) Putative nucleolar DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis YJL032W 1 386343 386657 OFF 10 YJL032W (386343..386657) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase YJL031C 0 386363 387433 ON 10 BET4 complement(386363..387343,387431..387433) Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p YJL030W 1 387652 388242 ON 10 MAD2 (387652..388242) Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p YJL029C 0 388380 390848 ON 10 VPS53 complement(388380..390848) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting YJL028W 1 391306 391641 OFF 10 YJL028W (391306..391641) Protein of unknown function; may interact with ribosomes, based on co-purification experiments YJL027C 0 391828 392244 OFF 10 YJL027C complement(391828..392244) Putative protein of unknown function YJL026W 1 392399 393598 ON 10 RNR2 (392399..393598) Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits YJL026C-A 0 393061 393282 OFF 10 YJL026C-A complement(393061..393282) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL026W/RNR2 YJL025W 1 393962 395506 ON 10 RRN7 (393962..395506) Protein involved in the transcription of 35S rRNA genes by RNA polymerase I; component of the core factor (CF) complex also composed of Rrn11p, Rrn6p and TATA-binding protein YJL024C 0 395926 396587 ON 10 APS3 complement(395926..396488,396566..396587) Small subunit of the clathrin-associated adaptor complex AP-3, which is involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function YJL023C 0 397350 398393 ON 10 PET130 complement(397350..398393) Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJL022W 1 398104 398412 OFF 10 YJL022W (398104..398412) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 YJL020C 0 398932 402405 ON 10 BBC1 complement(398932..402405) Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches YJL020W-A 1 400976 401233 OFF 10 YJL020W-A (400976..401233) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL020C/BBC1 YJL019W 1 402892 404940 ON 10 MPS3 (402892..404940) Essential integral membrane protein required for spindle pole body duplication and for nuclear fusion, localizes to the spindle pole body half bridge, interacts with DnaJ-like chaperone Jem1p and with centrin homolog Cdc31p YJL016W 1 405583 407268 ON 10 YJL016W (405583..407268) Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species YJL015C 0 407137 407511 OFF 10 YJL015C complement(407137..407511) Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W YJL014W 1 407552 409156 ON 10 CCT3 (407552..409156) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJL013C 0 409487 411034 ON 10 MAD3 complement(409487..411034) Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover YJL012C 0 411228 413393 ON 10 VTC4 complement(411228..413393) Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion YJL011C 0 414279 414764 ON 10 RPC17 complement(414279..414764) RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex YJL010C 0 417556 419556 ON 10 YJL010C complement(417556..419556) Essential nucleolar protein required for 18S rRNA synthesis YJL009W 1 419849 420175 OFF 10 YJL009W (419849..420175) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex YJL008C 0 419951 421657 ON 10 CCT8 complement(419951..421657) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJL007C 0 422692 423006 OFF 10 YJL007C complement(422692..423006) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJL006C 0 423132 424103 ON 10 CTK2 complement(423132..424103) Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins YJL005W 1 425151 431231 ON 10 CYR1 (425151..431231) Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation YJL004C 0 431583 432194 ON 10 SYS1 complement(431583..432194) Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation YJL003W 1 432638 432994 ON 10 COX16 (432638..432994) Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase YJL002C 0 433215 434645 ON 10 OST1 complement(433215..434645) Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins YJL001W 1 435157 435920 ON 10 PRE3 (435157..435221,435338..435920) 20S proteasome beta-type subunit, responsible for cleavage after acidic residues in peptides YJR001W 1 436796 438604 ON 10 AVT1 (436796..438604) Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YJR002W 1 438858 440639 ON 10 MPP10 (438858..440639) Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p YJR003C 0 440987 442546 ON 10 YJR003C complement(440987..442546) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR004C 0 442902 444854 ON 10 SAG1 complement(442902..444854) Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor YJR005W 1 445916 448018 ON 10 APL1 (445916..448018) Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex YJR005C-A 0 448470 448751 ON 10 YJR005C-A complement(448470..448751) Putative protein of unknown function, originally identified as a syntenic homolog of an Ashbya gossypii gene YJR006W 1 449195 450658 ON 10 POL31 (449195..450658) DNA polymerase III (delta) subunit, essential for cell viability; involved in DNA replication and DNA repair YJR007W 1 451013 451927 ON 10 SUI2 (451013..451927) Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP YJR008W 1 452423 453439 ON 10 YJR008W (452423..453439) Putative protein of unknown function; expression repressed by inosine and choline in an Opi1p-dependent manner; expression induced by mild heat-stress on a non-fermentable carbon source. YJR009C 0 453676 454674 ON 10 TDH2 complement(453676..454674) Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall YJR010W 1 456232 457767 ON 10 MET3 (456232..457767) ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism YJR010C-A 0 458070 458354 ON 10 SPC1 complement(458070..458354) Subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER), homolog of the SPC12 subunit of mammalian signal peptidase complex YJR011C 0 458634 459419 ON 10 YJR011C complement(458634..459419) Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS YJR012C 0 459788 460411 ON 10 YJR012C complement(459788..460411) Putative protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W YJR013W 1 460375 461586 ON 10 GPI14 (460375..461586) Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M YJR014W 1 461822 462418 ON 10 TMA22 (461822..462418) Protein of unknown function that associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; has homology to human protein DRP1, which interacts with human Tma20p homolog MCT-1 YJR015W 1 462714 464246 ON 10 YJR015W (462714..464246) Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene YJR016C 0 464444 466201 ON 10 ILV3 complement(464444..466201) Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids YJR017C 0 466514 467026 ON 10 ESS1 complement(466514..467026) Peptidylprolyl-cis/trans-isomerase (PPIase) specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNA polymerase II large subunit (Rpo21p) C-terminal domain YJR018W 1 466779 467141 OFF 10 YJR018W (466779..467141) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR019C 0 467225 468274 ON 10 TES1 complement(467225..468274) Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids YJR020W 1 467994 468326 OFF 10 YJR020W (467994..468326) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR021C 0 468548 469572 ON 10 REC107 complement(468548..469176,469257..469572) Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation YJR022W 1 469777 470106 ON 10 LSM8 (469777..470106) Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA YJR023C 0 469797 470198 OFF 10 YJR023C complement(469797..470198) Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing YJR024C 0 470223 470957 ON 10 YJR024C complement(470223..470957) Putative methylthio-ribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant YJR025C 0 471131 471664 ON 10 BNA1 complement(471131..471664) 3-hydroxyanthranilic acid dioxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway YJR030C 0 483952 486189 ON 10 YJR030C complement(483952..486189) Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; YJR030C is a non-essential gene YJR031C 0 486579 490805 ON 10 GEA1 complement(486579..490805) Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea2p YJR032W 1 491074 492255 ON 10 CPR7 (491074..492255) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity YJR033C 0 492371 496444 ON 10 RAV1 complement(492371..496444) Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate YJR034W 1 496676 497002 ON 10 PET191 (496676..497002) Protein required for assembly of cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR035W 1 497348 500605 ON 10 RAD26 (497348..500605) Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSB protein YJR036C 0 500706 503384 ON 10 HUL4 complement(500706..503384) Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability YJR037W 1 503095 503478 OFF 10 YJR037W (503095..503478) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; YJR037W is not an essential gene. YJR038C 0 503703 504065 OFF 10 YJR038C complement(503703..504065) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR039W 1 503929 507294 ON 10 YJR039W (503929..507294) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR040W 1 507739 510078 ON 10 GEF1 (507739..510078) Chloride channel localized to late- or post-Golgi vesicles, involved in iron metabolism; highly homologous to voltage-gated chloride channels in vertebrates YJR041C 0 510232 513756 ON 10 URB2 complement(510232..513756) Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis YJR042W 1 514048 516282 ON 10 NUP85 (514048..516282) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking YJR043C 0 516454 517506 ON 10 POL32 complement(516454..517506) Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p YJR044C 0 518756 519178 ON 10 VPS55 complement(518756..519178) Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) YJR045C 0 519631 521595 ON 10 SSC1 complement(519631..521595) Mitochondrial matrix ATPase; subunit of the presequence translocase-associated protein import motor (PAM) and of the SceI endonuclease; involved in protein translocation into the matrix and protein folding; member of HSP70 family YJR046W 1 522041 523855 ON 10 TAH11 (522041..523855) DNA replication licensing factor, required for pre-replication complex assembly YJR047C 0 524901 525374 ON 10 ANB1 complement(524901..525374) Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Hyp2p; undergoes an essential hypusination modification; expressed under anaerobic conditions YJR048W 1 526328 526657 ON 10 CYC1 (526328..526657) Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration YJR049C 0 526877 528469 ON 10 UTR1 complement(526877..528469) ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p) YJR050W 1 528690 529397 ON 10 ISY1 (528690..529397) Component of the spliceosome complex involved in pre-mRNA splicing, auxiliary splicing factor that may modulate Syf1p activity and help optimize splicing; isy1 syf2 double mutation activates the spindle checkpoint, causing cell cycle arrest YJR051W 1 529854 531359 ON 10 OSM1 (529854..531359) Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity YJR052W 1 532055 533752 ON 10 RAD7 (532055..533752) Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex YJR053W 1 534020 535744 ON 10 BFA1 (534020..535744) Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis YJR054W 1 536049 537542 ON 10 YJR054W (536049..537542) Vacuolar protein of unknown function; potential Cdc28p substrate YJR055W 1 538765 539259 ON 10 HIT1 (538765..539259) Protein of unknown function, required for growth at high temperature YJR056C 0 541785 542495 ON 10 YJR056C complement(541785..542495) Hypothetical protein YJR057W 1 544055 544705 ON 10 CDC8 (544055..544705) Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p YJR058C 0 544725 545168 ON 10 APS2 complement(544725..545168) Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex YJR059W 1 545780 548236 ON 10 PTK2 (545780..548236) Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake YJR060W 1 548752 549807 ON 10 CBF1 (548752..549807) Helix-loop-helix protein that binds the motif CACRTG, which is present at several sites including MET gene promoters and centromere DNA element I (CDEI); required for nucleosome positioning at this motif; targets Isw1p to DNA YJR061W 1 550504 553311 ON 10 YJR061W (550504..553311) Putative protein of unknown function; non-essential gene with similarity to Mnn4, a putative membrane protein involved in glycosylation; transcription repressed by Rm101p YJR062C 0 553469 554842 ON 10 NTA1 complement(553469..554842) Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation YJR063W 1 555188 555565 ON 10 RPA12 (555188..555565) RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex YJR064W 1 555907 557595 ON 10 CCT5 (555907..557595) Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YJR065C 0 557802 559151 ON 10 ARP3 complement(557802..559151) Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity YJR066W 1 559409 566821 ON 10 TOR1 (559409..566821) PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis YJR067C 0 567012 567437 ON 10 YAE1 complement(567012..567437) Essential protein of unknown function YJR068W 1 567636 568697 ON 10 RFC2 (567636..568697) Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon YJR069C 0 568799 569392 ON 10 HAM1 complement(568799..569392) Conserved protein with deoxyribonucleoside triphosphate pyrophosphohydrolase activity, mediates exclusion of noncanonical purines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine YJR070C 0 569614 570591 ON 10 LIA1 complement(569614..570591) Deoxyhypusine hydroxylase, a HEAT-repeat containing metalloenzyme that catalyses hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning YJR071W 1 570398 570766 OFF 10 YJR071W (570398..570766) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR072C 0 570960 572117 ON 10 NPA3 complement(570960..572117) Essential, conserved, cytoplasmic ATPase; phosphorylated by the Pcl1p-Pho85p kinase complex YJR073C 0 572308 572928 ON 10 OPI3 complement(572308..572928) Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis YJR074W 1 573088 573744 ON 10 MOG1 (573088..573744) Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p YJR075W 1 573974 575164 ON 10 HOC1 (573974..575164) Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele YJR076C 0 575347 576594 ON 10 CDC11 complement(575347..576594) Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM YJR077C 0 577248 578183 ON 10 MIR1 complement(577248..578183) Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2 under normal conditions YJR078W 1 578853 580214 ON 10 BNA2 (578853..580214) Tryptophan 2,3-dioxygenase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway YJR079W 1 580198 581232 OFF 10 YJR079W (580198..580340,581046..581232) Putative protein of unknown function; mutation results in impaired mitochondrial respiration YJR080C 0 580425 581609 ON 10 YJR080C complement(580425..581609) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR082C 0 581907 582248 ON 10 EAF6 complement(581907..582248) Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 YJR083C 0 582601 583530 ON 10 ACF4 complement(582601..583530) Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate YJR084W 1 583726 584997 ON 10 CSN12 (583726..584997) Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling YJR085C 0 585113 585430 ON 10 YJR085C complement(585113..585430) Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR086W 1 586061 586393 ON 10 STE18 (586061..586393) G protein gamma subunit, forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling YJR087W 1 586393 586743 OFF 10 YJR087W (586393..586743) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene STE18 and uncharacterized ORF YJR088C YJR088C 0 586488 587366 ON 10 YJR088C complement(586488..587366) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YJR089W 1 587711 590575 ON 10 BIR1 (587711..590575) Essential chromosomal passenger protein involved in coordinating cell cycle events for proper chromosome segregation; C-terminal region binds Sli15p, and the middle region, upon phosphorylation, localizes Cbf2p to the spindle at anaphase YJR090C 0 590865 594320 ON 10 GRR1 complement(590865..594320) F-box protein component of the SCF ubiquitin-ligase complex, required for Cln1p and Cln2p degradation; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, and morphogenesis YJR091C 0 595054 598329 ON 10 JSN1 complement(595054..598329) Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells YJR092W 1 598728 603074 ON 10 BUD4 (598728..603074) Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate YJR093C 0 603216 604199 ON 10 FIP1 complement(603216..604199) Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity YJR094C 0 604565 605647 ON 10 IME1 complement(604565..605647) Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p YJR094W-A 1 608301 608854 ON 10 RPL43B (608301..608302,608578..608854) Protein component of the large (60S) ribosomal subunit, identical to Rpl43Ap and has similarity to rat L37a ribosomal protein YJR095W 1 609769 610737 ON 10 SFC1 (609769..610737) Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization YJR096W 1 611191 612039 ON 10 YJR096W (611191..612039) Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YJR097W 1 612409 612927 ON 10 JJJ3 (612409..612927) Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein YJR098C 0 613182 615149 ON 10 YJR098C complement(613182..615149) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR099W 1 615569 616279 ON 10 YUH1 (615569..616279) Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p YJR100C 0 616344 617327 ON 10 YJR100C complement(616344..617327) Putative protein of unknown function; appears related to murine NOR1, which is highly expressed in leukemogenic cell lines; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YJR101W 1 618227 619027 ON 10 RSM26 (618227..619027) Mitochondrial ribosomal protein of the small subunit YJR102C 0 619150 619758 ON 10 VPS25 complement(619150..619758) Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome YJR103W 1 620747 622441 ON 10 URA8 (620747..622441) Minor CTP synthase isozyme (see also URA7), catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis YJR104C 0 622542 623006 ON 10 SOD1 complement(622542..623006) Cytosolic superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans YJR105W 1 623573 624595 ON 10 ADO1 (623573..624595) Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle YJR106W 1 624830 627007 ON 10 ECM27 (624830..627007) Non-essential protein of unknown function YJR107W 1 627333 628319 ON 10 YJR107W (627333..628319) Putative protein of unknown function; has sequence or structural similarity to lipases YJR108W 1 628706 629077 ON 10 ABM1 (628706..629077) Protein of unknown function, required for normal microtubule organization YJR109C 0 629579 632935 ON 10 CPA2 complement(629579..632935) Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor YJR110W 1 633609 635675 ON 10 YMR1 (633609..635675) Phosphatidylinositol 3-phosphate [PI(3)P] phosphatase, regulates the localization and levels of PI(3)P; involved in cytoplasm to vacuole (CVT) transport; has similarity to the conserved myotubularin dual specificity phosphatase family YJR111C 0 635849 636700 ON 10 YJR111C complement(635849..636700) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria YJR112W 1 637024 637629 ON 10 NNF1 (637024..637629) Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation YJR112W-A 1 637784 638162 ON 10 YJR112W-A (637784..637803,637853..638162) Putative protein of unknown function; identified based on homology to Ashbya gossypii YJR113C 0 638226 638969 ON 10 RSM7 complement(638226..638969) Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein YJR114W 1 638653 639045 OFF 10 YJR114W (638653..639045) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSM7/YJR113C YJR115W 1 639936 640445 ON 10 YJR115W (639936..640445) Putative protein of unknown function YJR116W 1 640819 641658 ON 10 YJR116W (640819..641658) Putative protein of unknown function YJR117W 1 642001 643362 ON 10 STE24 (642001..643362) Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans YJR118C 0 643484 644095 ON 10 ILM1 complement(643484..644095) Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth YJR119C 0 644298 646484 ON 10 JHD2 complement(644298..646484) JmjC domain family histone demethylase specific for H3-K4 (lysine at position 4 of the histone H3 protein); removes methyl groups specifically added by Set1p methyltransferase YJR120W 1 647120 647470 OFF 10 YJR120W (647120..647470) Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p YJR121W 1 647601 649136 ON 10 ATP2 (647601..649136) Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YJR122W 1 649770 651263 ON 10 CAF17 (649770..651263) Mitochondrial protein that interacts with Ccr4p in the two-hybrid system; 3'-untranslated region contains a putative mRNA localization element common to genes encoding mitochondrial proteins YJR123W 1 651895 652572 ON 10 RPS5 (651895..652572) Protein component of the small (40S) ribosomal subunit, the least basic of the non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; has similarity to E. coli S7 and rat S5 ribosomal proteins YJR124C 0 652886 654232 ON 10 YJR124C complement(652886..654232) Putative protein of unknown function; expression induced under calcium shortage YJR125C 0 654731 655957 ON 10 ENT3 complement(654731..655957) Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p YJR126C 0 656248 658683 ON 10 VPS70 complement(656248..658683) Protein of unknown function involved in vacuolar protein sorting YJR127C 0 658911 663053 ON 10 RSF2 complement(658911..663053) Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions YJR128W 1 662915 663274 OFF 10 YJR128W (662915..663274) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 YJR129C 0 663994 665013 ON 10 YJR129C complement(663994..665013) Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR130C 0 665212 667131 ON 10 STR2 complement(665212..667131) Cystathionine gamma-synthase, converts cysteine into cystathionine YJR131W 1 667638 669287 ON 10 MNS1 (667638..669287) Alpha-1,2-mannosidase involved in ER quality control; catalyzes the removal of one mannose residue from Man9GlcNAc to produce a single isomer of Man8GlcNAc in N-linked oligosaccharide biosynthesis; integral to ER membrane YJR132W 1 669516 672662 ON 10 NMD5 (669516..672662) Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog YJR133W 1 672985 673614 ON 10 XPT1 (672985..673614) Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine YJR134C 0 673723 675846 ON 10 SGM1 complement(673723..675846) Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus YJR135C 0 676053 676772 ON 10 MCM22 complement(676053..676772) Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner YJR135W-A 1 676965 677228 ON 10 TIM8 (676965..677228) Mitochondrial intermembrane space protein mediating import and insertion of polytopic inner membrane proteins; homolog of human DDP1 (deafness dystonia peptide 1) which is mutated in the X-linked Mohr-Tranebjaerg syndrome YJR136C 0 677435 678700 ON 10 YJR136C complement(677435..678700) Putative protein of unknown function; may interact with mitochondrial ribosomal protein Rsm23p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR137C 0 678951 683279 ON 10 ECM17 complement(678951..683279) Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine YJR138W 1 684561 689315 ON 10 IML1 (684561..689315) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane YJR139C 0 689439 690518 ON 10 HOM6 complement(689439..690518) Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions YJR140C 0 690744 695690 ON 10 HIR3 complement(690744..695690) Transcriptional corepressor involved in the cell cycle-regulated transcription of histone genes HTA1, HTB1, HHT1, and HHT2; involved in position-dependent gene silencing and nucleosome reassembly YJR140W-A 1 690955 691104 OFF 10 YJR140W-A (690955..691104) Dubious open reading frame; completely overlaps the verified gene HIR3; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YJR141W 1 695900 696943 ON 10 YJR141W (695900..696943) Putative protein of unknown function; may be involved in mRNA processing; YJR141W is an essential gene YJR142W 1 697135 698163 ON 10 YJR142W (697135..698163) Putative protein of unknown function; GST fusion protein is a Dbf2-Mob1 phosphoylation target in a proteome chip analysis; synthetic lethal with PH085 deletion YJR143C 0 698320 700608 ON 10 PMT4 complement(698320..700608) Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals YJR144W 1 700876 701685 ON 10 MGM101 (700876..701685) Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage YJR145C 0 702021 703062 ON 10 RPS4A complement(702021..702792,703049..703062) Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein YJR146W 1 703879 704232 OFF 10 YJR146W (703879..704232) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HMS2 YJR147W 1 704190 705266 ON 10 HMS2 (704190..705266) Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant YJR148W 1 705738 706868 ON 10 BAT2 (705738..706868) Cytosolic branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase YJR149W 1 707154 708368 ON 10 YJR149W (707154..708368) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR150C 0 708805 709701 ON 10 DAN1 complement(708805..709701) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YJR151C 0 712249 715734 ON 10 DAN4 complement(712249..715734) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YJR151W-A 1 717574 717624 OFF 10 YJR151W-A (717574..717624) Putative protein of unknown function; identified by fungal homology and RT-PCR YJR152W 1 719660 721291 ON 10 DAL5 (719660..721291) Allantoin permease; ureidosuccinate permease; expression is constitutive but sensitive to nitrogen catabolite repression YJR153W 1 722809 723894 ON 10 PGU1 (722809..723894) Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins YJR154W 1 725778 726818 ON 10 YJR154W (725778..726818) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YJR155W 1 727399 728265 ON 10 AAD10 (727399..728265) Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role YJR156C 0 728568 729590 ON 10 THI11 complement(728568..729590) Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 YJR157W 1 730509 730871 OFF 10 YJR157W (730509..730871) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YJR158W 1 732434 734137 ON 10 HXT16 (732434..734137) Protein of unknown function with similarity to hexose transporter family members, expression is repressed by high levels of glucose YJR159W 1 736038 737111 ON 10 SOR1 (736038..737111) Sorbitol dehydrogenase; expression is induced in the presence of sorbitol YJR160C 0 738002 739810 ON 10 MPH3 complement(738002..739810) Alpha-glucoside permease, transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication YJR161C 0 742842 743993 ON 10 COS5 complement(742842..743993) Protein of unknown function, member the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YJR162C 0 744905 745255 OFF 10 YJR162C complement(744905..745255) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL225W 1 452 799 OFF 11 YKL225W (452..799) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL224C 0 1811 2182 ON 11 YKL224C complement(1811..2182) Putative protein of unknown function YKL223W 1 2390 2722 OFF 11 YKL223W (2390..2722) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL222C 0 3504 5621 ON 11 YKL222C complement(3504..5621) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine YKL221W 1 6108 7529 ON 11 MCH2 (6108..7529) Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport YKL220C 0 9092 11227 ON 11 FRE2 complement(9092..11227) Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels YKL219W 1 14485 15708 ON 11 COS9 (14485..15708) Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YKL218C 0 17359 18339 ON 11 SRY1 complement(17359..18339) 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required for survival in the presence of hydroxyaspartate YKL217W 1 22234 24084 ON 11 JEN1 (22234..24084) Lactate transporter, required for uptake of lactate and pyruvate; expression is derepressed by transcriptional activator Cat8p under nonfermentative growth conditions, and repressed in the presence of glucose, fructose, and mannose YKL216W 1 25216 26160 ON 11 URA1 (25216..26160) Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid YKL215C 0 26828 30688 ON 11 YKL215C complement(26828..30688) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL214C 0 31083 31694 ON 11 YRA2 complement(31083..31694) Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus YKL213C 0 31961 34108 ON 11 DOA1 complement(31961..34108) WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase YKL212W 1 34544 36415 ON 11 SAC1 (34544..36415) Lipid phosphoinositide phosphatase of the ER and Golgi, involved in protein trafficking and secretion YKL211C 0 36700 38154 ON 11 TRP3 complement(36700..38154) Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p YKL210W 1 39164 42238 ON 11 UBA1 (39164..42238) Ubiquitin activating enzyme (E1), involved in ubiquitin-mediated protein degradation and essential for viability YKL209C 0 42424 46296 ON 11 STE6 complement(42424..46296) Plasma membrane ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells YKL208W 1 47158 47973 ON 11 CBT1 (47158..47973) Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p YKL207W 1 48195 48956 ON 11 YKL207W (48195..48956) Putative protein of unknown function; non-essential gene YKL206C 0 49007 49810 ON 11 ADD66 complement(49007..49810) Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly YKL205W 1 50052 53354 ON 11 LOS1 (50052..53354) Nuclear pore protein involved in nuclear export of pre-tRNA YKL204W 1 53705 55603 ON 11 EAP1 (53705..55603) eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade YKL203C 0 55936 63360 ON 11 TOR2 complement(55936..63360) PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis YKL202W 1 63822 64403 OFF 11 YKL202W (63822..64403) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL201C 0 63931 67467 ON 11 MNN4 complement(63931..67467) Putative positive regulator of mannosylphosphate transferase (Mnn6p), involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases YKL198C 0 68274 70223 ON 11 PTK1 complement(68274..70223) Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein YKL197C 0 70739 73870 ON 11 PEX1 complement(70739..73870) AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis YKL196C 0 74937 75539 ON 11 YKT6 complement(74937..75539) Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus YKL195W 1 75826 77037 ON 11 MIA40 (75826..77037) Essential protein of the mitochondrial intermembrane space (IMS); promotes retention of newly imported proteins; may do so by stabilizing client protein folding as part of a disulfide relay system or transferring metal to client proteins YKL194C 0 77263 78651 ON 11 MST1 complement(77263..78651) Mitochondrial threonyl-tRNA synthetase YKL193C 0 78871 79887 ON 11 SDS22 complement(78871..79887) Conserved nuclear regulatory subunit of Glc7p type 1 protein serine-threonine phosphatase (PP1), functions positively with Glc7p to promote dephosphorylation of nuclear substrates required for chromosome transmission during mitosis YKL192C 0 80165 80542 ON 11 ACP1 complement(80165..80542) Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p YKL191W 1 81040 82644 ON 11 DPH2 (81040..82644) Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p YKL190W 1 82952 83555 ON 11 CNB1 (82952..83003,83080..83555) Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 YKL189W 1 84709 85908 ON 11 HYM1 (84709..85908) Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response YKL188C 0 86230 88791 ON 11 PXA2 complement(86230..88791) Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins YKL187C 0 89289 91541 ON 11 YKL187C complement(89289..91541) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKL186C 0 92749 93303 ON 11 MTR2 complement(92749..93303) mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA YKL185W 1 94504 96270 ON 11 ASH1 (94504..96270) Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate YKL184W 1 96762 98162 ON 11 SPE1 (96762..98162) Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines YKL183C-A 0 98400 98612 OFF 11 YKL183C-A complement(98400..98612) Putative protein of unknown function; identified by fungal homology and RT-PCR YKL183W 1 98726 99646 ON 11 LOT5 (98726..99646) Protein of unknown function; gene expression increases in cultures shifted to a lower temperature YKL182W 1 100676 106831 ON 11 FAS1 (100676..106831) Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities YKL181W 1 107321 108604 ON 11 PRS1 (107321..108604) 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes YKL180W 1 109274 110134 ON 11 RPL17A (109274..109582,109889..110134) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the Dam1 complex (aka DASH complex) YKL179C 0 110469 112508 ON 11 COY1 complement(110469..112508) Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function YKL178C 0 113220 114632 ON 11 STE3 complement(113220..114632) Receptor for a factor receptor, transcribed in alpha cells and required for mating by alpha cells, couples to MAP kinase cascade to mediate pheromone response; ligand bound receptors are endocytosed and recycled to the plasma membrane; GPCR YKL177W 1 114361 114699 OFF 11 YKL177W (114361..114699) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3 YKL176C 0 115505 117991 ON 11 LST4 complement(115505..117991) Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface YKL175W 1 118798 120309 ON 11 ZRT3 (118798..120309) Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency YKL174C 0 120385 122241 ON 11 TPO5 complement(120385..122241) Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles YKL173W 1 122522 125548 ON 11 SNU114 (122522..125548) GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 YKL172W 1 125764 127047 ON 11 EBP2 (125764..127047) Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles YKL171W 1 127480 130266 ON 11 YKL171W (127480..130266) Putative protein of unknown function; predicted protein kinase; implicated in proteasome function; epitope-tagged protein localizes to the cytoplasm YKL170W 1 130639 131055 ON 11 MRPL38 (130639..131055) Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels YKL169C 0 130690 131073 OFF 11 YKL169C complement(130690..131073) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 YKL168C 0 131293 133467 ON 11 KKQ8 complement(131293..133467) Putative serine/threonine protein kinase with unknown cellular role YKL167C 0 133726 134139 ON 11 MRP49 complement(133726..134139) Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation YKL166C 0 134514 135710 ON 11 TPK3 complement(134514..135710) cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; partially redundant with Tpk1p and Tpk2p YKL165C-A 0 135795 136028 OFF 11 YKL165C-A complement(135795..136028) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKL165C 0 137937 140696 ON 11 MCD4 complement(137937..140696) Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes YKL164C 0 141799 142824 ON 11 PIR1 complement(141799..142824) O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle YKL163W 1 144406 145383 ON 11 PIR3 (144406..145383) O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway YKL162C-A 0 145927 146079 OFF 11 YKL162C-A complement(145927..146079) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL162C 0 147635 148843 ON 11 YKL162C complement(147635..148843) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion YKL161C 0 149391 150692 ON 11 YKL161C complement(149391..150692) Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; associates with Rlm1p YKL160W 1 153274 153711 ON 11 ELF1 (153274..153711) Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression YKL159C 0 153821 154456 ON 11 RCN1 complement(153821..154456) Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region YKL157W 1 154996 158186 ON 11 APE2 (154996..155276,155660..158186) Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W YKL156W 1 158619 159217 ON 11 RPS27A (158619..158621,158972..159217) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein YKL156C-A 0 158980 159096 OFF 11 YKL156C-A complement(158980..159096) Dubious open reading frame unlikely to encode a protein; completely overlaps the verified gene RPS27A; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKL155C 0 159461 161347 ON 11 RSM22 complement(159461..161347) Mitochondrial ribosomal protein of the small subunit YKL154W 1 161606 162340 ON 11 SRP102 (161606..162340) Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors Srp101p to the ER membrane YKL153W 1 163605 164114 OFF 11 YKL153W (163605..164114) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant YKL152C 0 163647 164390 ON 11 GPM1 complement(163647..164390) Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis YKL151C 0 164922 165935 ON 11 YKL151C complement(164922..165935) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL150W 1 166549 167457 ON 11 MCR1 (166549..167457) Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis YKL149C 0 167617 168834 ON 11 DBR1 complement(167617..168834) RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition YKL148C 0 169212 171134 ON 11 SDH1 complement(169212..171134) Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone YKL147C 0 171556 172173 OFF 11 YKL147C complement(171556..172173) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 YKL146W 1 171788 173866 ON 11 AVT3 (171788..173866) Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters YKL145W 1 174218 175621 ON 11 RPT1 (174218..175621) One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p YKL145W-A 1 174963 175055 OFF 11 YKL145W-A (174963..175055) Dubious open reading frame, unlikely to encode a protein; completely overlaps the verified essential gene RPT1; identified by expression profiling and mass spectrometry YKL144C 0 175848 176486 ON 11 RPC25 complement(175848..176486) RNA polymerase III subunit C25 YKL143W 1 176786 178177 ON 11 LTV1 (176786..178177) Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature YKL142W 1 178520 179179 ON 11 MRP8 (178520..179179) Putative mitochondrial ribosomal protein, has similarity to E. coli ribosomal protein S2 YKL141W 1 179672 180268 ON 11 SDH3 (179672..180268) Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone YKL140W 1 180784 182430 ON 11 TGL1 (180784..182430) Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes YKL139W 1 182963 184549 ON 11 CTK1 (182963..184549) Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing YKL138C-A 0 184808 185017 ON 11 HSK3 complement(184808..185017) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YKL138C 0 185291 185686 ON 11 MRPL31 complement(185291..185686) Mitochondrial ribosomal protein of the large subunit YKL137W 1 185962 186297 ON 11 YKL137W (185962..186297) Putative protein of unknown function; mutation results in growth defect on a non-fermentable (respiratory) carbon source YKL136W 1 186421 186819 OFF 11 YKL136W (186421..186819) Hypothetical protein YKL135C 0 186465 188645 ON 11 APL2 complement(186465..188645) Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting YKL134C 0 189129 191441 ON 11 OCT1 complement(189129..191441) Mitochondrial intermediate peptidase, cleaves N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis YKL133C 0 191678 193069 ON 11 YKL133C complement(191678..193069) Putative protein of unknown function YKL132C 0 193573 194865 ON 11 RMA1 complement(193573..194865) Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKL131W 1 194477 194998 OFF 11 YKL131W (194477..194998) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL130C 0 195287 196027 ON 11 SHE2 complement(195287..196027) RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud YKL129C 0 196348 200163 ON 11 MYO3 complement(196348..200163) One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization YKL128C 0 200528 201415 ON 11 PMU1 complement(200528..201415) Putative phosphomutase, contains a region homologous to the active site of phsophomutases; overexpression of the gene suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant YKL127W 1 203185 204897 ON 11 PGM1 (203185..204897) Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism YKL126W 1 205351 207393 ON 11 YPK1 (205351..207393) Serine/threonine protein kinase required for receptor-mediated endocytosis; involved in sphingolipid-mediated and cell integrity signaling pathways; localized to the bud neck, cytosol and plasma membrane; homolog of mammalian kinase SGK YKL125W 1 207891 209774 ON 11 RRN3 (207891..209774) Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA YKL124W 1 210237 211976 ON 11 SSH4 (210237..211976) Vacuolar protein that presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane YKL123W 1 211687 212067 OFF 11 YKL123W (211687..212067) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 YKL122C 0 212139 212642 ON 11 SRP21 complement(212139..212642) Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm YKL121W 1 213786 216344 ON 11 YKL121W (213786..216344) Putative protein of unknown function YKL120W 1 216988 217962 ON 11 OAC1 (216988..217962) Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, and thiosulfate; member of the mitochondrial carrier family YKL119C 0 218214 218861 ON 11 VPH2 complement(218214..218861) Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) YKL118W 1 218770 219081 OFF 11 YKL118W (218770..219081) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 YKL117W 1 219968 220618 ON 11 SBA1 (219968..220618) Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins and like p23 can regulate telomerase activity YKL116C 0 220988 222544 ON 11 PRR1 complement(220988..222544) Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor YKL115C 0 222537 222929 OFF 11 YKL115C complement(222537..222929) Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 YKL114C 0 222996 224099 ON 11 APN1 complement(222996..224099) Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine YKL113C 0 224371 225519 ON 11 RAD27 complement(224371..225519) 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family YKL112W 1 226214 228409 ON 11 ABF1 (226214..228409) DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair YKL111C 0 228108 228443 OFF 11 YKL111C complement(228108..228443) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 YKL110C 0 228583 229524 ON 11 KTI12 complement(228583..229524) Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p YKL109W 1 231871 233535 ON 11 HAP4 (231871..233535) Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex YKL108W 1 234070 235431 ON 11 SLD2 (234070..235431) Protein required for DNA replication, phosphorylated in S phase by S-phase cyclin-dependent kinases (Cdks), phosphorylation is essential for DNA replication and for complex formation with Dpb11p; potential Cdc28p substrate YKL107W 1 235785 236714 ON 11 YKL107W (235785..236714) Putative protein of unknown function YKL106C-A 0 236791 236910 OFF 11 YKL106C-A complement(236791..236910) Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi YKL106W 1 237180 238535 ON 11 AAT1 (237180..238535) Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis YKL105C 0 238829 242227 ON 11 YKL105C complement(238829..242227) Putative protein of unknown function YKL104C 0 242864 245017 ON 11 GFA1 complement(242864..245017) Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis YKL103C 0 245782 247326 ON 11 LAP4 complement(245782..247326) Vacuolar aminopeptidase, often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway YKL102C 0 247706 248011 OFF 11 YKL102C complement(247706..248011) Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site YKL101W 1 248564 253120 ON 11 HSL1 (248564..253120) Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p YKL100C 0 253341 255104 ON 11 YKL100C complement(253341..255104) Putative protein of unknown function with similarity to a human minor histocompatibility antigen; YKL100C is not an essential gene YKL100W-A 1 253803 253892 OFF 11 YKL100W-A (253803..253892) Putative protein of unknown function; identified by expression profiling and mass spectrometry YKL099C 0 255364 256116 ON 11 UTP11 complement(255364..256116) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YKL098W 1 256414 257487 ON 11 YKL098W (256414..257487) Putative protein of unknown function YKL097C 0 258147 258557 OFF 11 YKL097C complement(258147..258557) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species YKL096C-B 0 258717 258866 OFF 11 YKL096C-B complement(258717..258866) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKL096W-A 1 258897 259175 ON 11 CWP2 (258897..259175) Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored YKL096W 1 260776 261495 ON 11 CWP1 (260776..261495) Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization YKL095W 1 261921 262757 ON 11 YJU2 (261921..262757) Essential nuclear protein; putative spliceosomal component involved in mRNA splicing, based on computational analysis of large-scale protein-protein interaction data YKL094W 1 262993 263934 ON 11 YJU3 (262993..263934) Serine hydrolase with sequence similarity to monoglyceride lipase (MGL), localizes to lipid particles YKL093W 1 264433 265452 ON 11 MBR1 (264433..265452) Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants YKL092C 0 265789 269103 ON 11 BUD2 complement(265789..269103) GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types YKL091C 0 269362 270294 ON 11 YKL091C complement(269362..270294) Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus YKL090W 1 271522 272853 ON 11 CUE2 (271522..272853) Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination YKL089W 1 273038 274687 ON 11 MIF2 (273038..274687) Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p YKL088W 1 274927 276642 ON 11 YKL088W (274927..276642) Predicted phosphopantothenoylcysteine decarboxylase, may be involved in coenzyme A biosynthesis; interacts with Sis2p and Vhs3p YKL087C 0 276833 277507 ON 11 CYT2 complement(276833..277507) Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 YKL086W 1 277925 278308 ON 11 SRX1 (277925..278308) Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxins Tsa1p and Ahp1p that are formed upon exposure to oxidants; conserved in higher eukaryotes YKL085W 1 278767 279771 ON 11 MDH1 (278767..279771) Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle YKL084W 1 280153 280503 ON 11 HOT13 (280153..280503) Mitochondrial intermembrane space protein, first component of a pathway mediating assembly of small TIM (Translocase of the Inner Membrane) complexes which escort hydrophobic inner membrane proteins en route to the TIM22 complex YKL083W 1 280565 281179 OFF 11 YKL083W (280565..281179) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 YKL082C 0 280669 281973 ON 11 RRP14 complement(280669..281973) Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family YKL081W 1 282535 284099 ON 11 TEF4 (282535..282739,283066..284099) Translation elongation factor EF-1 gamma YKL080W 1 284674 285852 ON 11 VMA5 (284674..285852) Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane YKL079W 1 286247 288217 ON 11 SMY1 (286247..288217) Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins YKL078W 1 288489 290696 ON 11 DHR2 (288489..290696) Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis YKL077W 1 291097 292275 ON 11 YKL077W (291097..292275) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole YKL076C 0 292482 292865 OFF 11 PSY1 complement(292482..292865) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C YKL075C 0 292600 293952 ON 11 YKL075C complement(292600..293952) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin YKL074C 0 294254 295837 ON 11 MUD2 complement(294254..295837) Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 YKL073W 1 296074 298719 ON 11 LHS1 (296074..298719) Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway YKL072W 1 299226 301526 ON 11 STB6 (299226..301526) Protein that binds Sin3p in a two-hybrid assay YKL071W 1 304758 305528 ON 11 YKL071W (304758..305528) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL070W 1 305855 306364 ON 11 YKL070W (305855..306364) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKL069W 1 306929 307471 ON 11 YKL069W (306929..307471) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YKL068W-A 1 308850 309086 ON 11 YKL068W-A (308850..309086) Putative protein of unknown function; identified by homology to Ashbya gossypii YKL068W 1 309843 312722 ON 11 NUP100 (309843..312722) Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup116p YKL067W 1 314456 314917 ON 11 YNK1 (314456..314917) Nucleoside diphosphate kinase, catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate YKL066W 1 314925 315368 OFF 11 YKL066W (314925..315368) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 YKL065W-A 1 315598 315819 ON 11 YKL065W-A (315598..315819) Putative protein of unknown function YKL065C 0 316081 316701 ON 11 YET1 complement(316081..316701) Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein YKL064W 1 317408 320317 ON 11 MNR2 (317408..320317) Putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations YKL063C 0 320659 321162 ON 11 YKL063C complement(320659..321162) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi YKL062W 1 322872 324764 ON 11 MSN4 (322872..324764) Transcriptional activator related to Msn2p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression YKL061W 1 325415 325756 ON 11 YKL061W (325415..325756) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome YKL060C 0 326052 327131 ON 11 FBA1 complement(326052..327131) Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress YKL059C 0 327762 329087 ON 11 MPE1 complement(327762..329087) Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif YKL058W 1 329810 330178 ON 11 TOA2 (329810..330178) TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA YKL057C 0 330500 333613 ON 11 NUP120 complement(330500..333613) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p YKL056C 0 334056 334559 ON 11 TMA19 complement(334056..334559) Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress YKL055C 0 334965 335801 ON 11 OAR1 complement(334965..335801) Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p YKL054C 0 336181 338397 ON 11 DEF1 complement(336181..338397) RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex YKL053C-A 0 338826 339086 ON 11 MDM35 complement(338826..339086) Mitochondrial intermembrane space cysteine motif protein; mutation affects mitochondrial distribution and morphology YKL053W 1 339216 339590 OFF 11 YKL053W (339216..339590) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 YKL052C 0 339312 340190 ON 11 ASK1 complement(339312..340190) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases YKL051W 1 340956 342017 ON 11 SFK1 (340956..342017) Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane YKL050C 0 342496 345264 ON 11 YKL050C complement(342496..345264) Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p YKL049C 0 345719 346408 ON 11 CSE4 complement(345719..346408) Centromere protein that resembles histones, required for proper kinetochore function; homolog of human CENP-A YKL048C 0 346858 348780 ON 11 ELM1 complement(346858..348780) Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring YKL047W 1 349107 350657 ON 11 YKL047W (349107..350657) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL046C 0 350919 352268 ON 11 DCW1 complement(350919..352268) Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p YKL045W 1 353137 354723 ON 11 PRI2 (353137..354723) Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair YKL044W 1 355966 356286 OFF 11 YKL044W (355966..356286) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YKL043W 1 356392 357492 ON 11 PHD1 (356392..357492) Transcriptional activator that enhances pseudohyphal growth; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate YKL042W 1 358119 359210 ON 11 SPC42 (358119..359210) Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane YKL041W 1 359786 360460 ON 11 VPS24 (359786..360460) One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway YKL040C 0 360701 361471 ON 11 NFU1 complement(360701..361471) Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria YKL039W 1 362265 363836 ON 11 PTM1 (362265..363836) Protein of unknown function, copurifies with late Golgi vesicles containing the v-SNARE Tlg2p YKL038W 1 365248 368760 ON 11 RGT1 (365248..368760) Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor YKL037W 1 369008 369364 OFF 11 YKL037W (369008..369364) Putative protein of unknown function YKL036C 0 369171 369563 OFF 11 YKL036C complement(369171..369563) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W YKL035W 1 369534 371033 ON 11 UGP1 (369534..371033) UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p YKL034W 1 371472 373748 ON 11 TUL1 (371472..373748) Golgi-localized RING-finger ubiquitin ligase (E3), involved in ubiquitinating and sorting membrane proteins that contain polar transmembrane domains to multivesicular bodies for delivery to the vacuole for quality control purposes YKL033W-A 1 374148 374858 ON 11 YKL033W-A (374148..374858) Putative protein of unknown function; similar to uncharacterized proteins from other fungi YKL033W 1 375099 378215 ON 11 YKL033W (375099..378215) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YKL033W is an essential gene YKL032C 0 379708 381501 ON 11 IXR1 complement(379708..381501) Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b YKL031W 1 381715 382128 OFF 11 YKL031W (381715..382128) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species YKL030W 1 382141 382746 OFF 11 YKL030W (382141..382746) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 YKL029C 0 382359 384368 ON 11 MAE1 complement(382359..384368) Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids YKL028W 1 385401 386849 ON 11 TFA1 (385401..386849) TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening YKL027W 1 387205 388548 ON 11 YKL027W (387205..388548) Protein of unknown function, localized to the mitochondrial outer membrane YKL026C 0 389023 389526 ON 11 GPX1 complement(389023..389526) Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress YKL025C 0 389883 391922 ON 11 PAN3 complement(389883..391922) Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes YKL024C 0 392169 392783 ON 11 URA6 complement(392169..392783) Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) YKL023C-A 0 392789 393016 OFF 11 YKL023C-A complement(392789..393016) Putative protein of unknown function YKL023W 1 393364 394197 ON 11 YKL023W (393364..394197) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL022C 0 394369 396891 ON 11 CDC16 complement(394369..396891) Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation YKL021C 0 396987 398393 ON 11 MAK11 complement(396987..398393) Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats YKL020C 0 398475 401723 ON 11 SPT23 complement(398475..401723) ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting YKL019W 1 402211 403161 ON 11 RAM2 (402211..403161) Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor YKL018C-A 0 403223 403522 ON 11 YKL018C-A complement(403223..403522) Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKL018W 1 403745 404734 ON 11 SWD2 (403745..404734) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination YKL017C 0 404833 406884 ON 11 HCS1 complement(404833..406884) Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities YKL016C 0 407108 407632 ON 11 ATP7 complement(407108..407632) Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YKL015W 1 408187 411126 ON 11 PUT3 (408187..411126) Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences and undergoes a conformational change to form the active state; has a Zn(2)-Cys(6) binuclear cluster domain YKL014C 0 411262 416556 ON 11 URB1 complement(411262..416556) Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit YKL013C 0 417151 417666 ON 11 ARC19 complement(417151..417666) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YKL012W 1 417953 419704 ON 11 PRP40 (417953..419704) U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex YKL011C 0 419798 420859 ON 11 CCE1 complement(419798..420859) Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA YKL010C 0 421067 425518 ON 11 UFD4 complement(421067..425518) Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins YKL009W 1 425885 426595 ON 11 MRT4 (425885..426595) Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus YKL008C 0 426938 428194 ON 11 LAC1 complement(426938..428194) Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p YKL007W 1 428945 429751 ON 11 CAP1 (428945..429751) Alpha subunit of the capping protein (CP) heterodimer (Cap1p and Cap2p) which binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches YKL006C-A 0 429841 430275 ON 11 SFT1 complement(429841..430098,430240..430275) Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment YKL006W 1 431549 432363 ON 11 RPL14A (431549..431677,432076..432363) N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth YKL005C 0 432736 434520 ON 11 BYE1 complement(432736..434520) Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit YKL004W 1 435223 436428 ON 11 AUR1 (435223..436428) Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance YKL003C 0 436740 437135 ON 11 MRP17 complement(436740..437135) Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator YKL002W 1 437421 438187 ON 11 DID4 (437421..437480,437549..438187) Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis YKL001C 0 438420 439028 ON 11 MET14 complement(438420..439028) Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism YKR001C 0 440251 442365 ON 11 VPS1 complement(440251..442365) Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, late Golgi-retention of some proteins, regulating peroxisome biogenesis YKR002W 1 442875 444581 ON 11 PAP1 (442875..444581) Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs YKR003W 1 445024 446370 ON 11 OSH6 (445024..446370) Member of an oxysterol-binding protein family with overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery YKR004C 0 446442 447679 ON 11 ECM9 complement(446442..447349,447454..447679) Non-essential protein of unknown function YKR005C 0 448164 449507 ON 11 YKR005C complement(448164..449507) Putative protein of unknown function YKR006C 0 450066 450860 ON 11 MRPL13 complement(450066..450860) Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation YKR007W 1 451077 451631 ON 11 MEH1 (451077..451631) Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification YKR008W 1 451844 453721 ON 11 RSC4 (451844..453721) Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling YKR009C 0 453995 456697 ON 11 FOX2 complement(453995..456697) Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities YKR010C 0 458567 460882 ON 11 TOF2 complement(458567..460882) Nonessential mitochondrial protein of unknown function with sequence similarity to Net1p; identified as a topoisomerase I (Top1p) binding protein; displays synthetic genetic interactions with TOP1 and HPR1 YKR011C 0 461278 462339 ON 11 YKR011C complement(461278..462339) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YKR012C 0 463382 463759 OFF 11 YKR012C complement(463382..463759) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 YKR013W 1 463602 464591 ON 11 PRY2 (463602..464591) Protein of unknown function, has similarity to Pry1p and Pry3p and to the plant PR-1 class of pathogen related proteins YKR014C 0 465010 465714 ON 11 YPT52 complement(465010..465714) GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis YKR015C 0 466604 468310 ON 11 YKR015C complement(466604..468310) Putative protein of unknown function YKR016W 1 469360 470982 ON 11 YKR016W (469360..470982) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR017C 0 471337 472992 ON 11 YKR017C complement(471337..472992) Putative protein of unknown function; contains a RING finger motif YKR018C 0 473366 475543 ON 11 YKR018C complement(473366..475543) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YKR019C 0 475859 477706 ON 11 IRS4 complement(475859..477706) Protein involved in regulation of phosphatidylinositol 4,5-bisphosphate concentrations; Irs4p and Tax4p bind and activate the phosphatase Inp51p; mutation confers an increase in rDNA silencing YKR020W 1 477981 478475 ON 11 VPS51 (477981..478475) Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p YKR021W 1 478877 481624 ON 11 YKR021W (478877..481624) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKR022C 0 481814 482782 ON 11 NTR2 complement(481814..482782) Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly YKR023W 1 483062 484654 ON 11 YKR023W (483062..484654) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR024C 0 484787 487015 ON 11 DBP7 complement(484787..487015) Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis YKR025W 1 487413 488261 ON 11 RPC37 (487413..488261) RNA polymerase III subunit C37 YKR026C 0 488381 489298 ON 11 GCN3 complement(488381..489298) Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression YKR027W 1 491007 493304 ON 11 BCH2 (491007..493304) Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane YKR028W 1 493900 497196 ON 11 SAP190 (493900..497196) Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p YKR029C 0 497220 499475 ON 11 SET3 complement(497220..499475) Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains YKR030W 1 499924 500745 ON 11 GMH1 (499924..500745) Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting YKR031C 0 500986 506037 ON 11 SPO14 complement(500986..506037) Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis YKR032W 1 506159 506473 OFF 11 YKR032W (506159..506473) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKR033C 0 506521 506946 OFF 11 YKR033C complement(506521..506946) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 YKR034W 1 506540 507349 ON 11 DAL80 (506540..507349) Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Deh1p YKR035C 0 507562 508203 OFF 11 OPI8 complement(507562..508203) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A YKR035W-A 1 507581 508195 ON 11 DID2 (507581..508195) Class E protein of the vacuolar protein-sorting (Vps) pathway, associates reversibly with the late endosome, has human ortholog that may be altered in breast tumors YKR036C 0 508344 510275 ON 11 CAF4 complement(508344..510275) WD40 repeat-containing protein associated with the CCR4-NOT complex, interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission YKR037C 0 510552 511439 ON 11 SPC34 complement(510552..511439) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body YKR038C 0 511641 512801 ON 11 KAE1 complement(511641..512801) Putative glycoprotease proposed to be in transcription as a component of the EKC protein complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; also identified as a component of the KEOPS protein complex YKR039W 1 514705 516513 ON 11 GAP1 (514705..516513) General amino acid permease; localization to the plasma membrane is regulated by nitrogen source YKR040C 0 517705 518208 OFF 11 YKR040C complement(517705..518208) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W YKR041W 1 517840 518592 ON 11 YKR041W (517840..518592) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YKR042W 1 519169 520266 ON 11 UTH1 (519169..520266) Mitochondrial outer membrane and cell wall localized SUN family member required for mitochondrial autophagy; involved in the oxidative stress response, life span during starvation, mitochondrial biogenesis, and cell death YKR043C 0 520539 521354 ON 11 YKR043C complement(520539..521354) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus YKR044W 1 521657 522988 ON 11 UIP5 (521657..522988) Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates YKR045C 0 523060 523611 ON 11 YKR045C complement(523060..523611) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YKR046C 0 523865 524716 ON 11 PET10 complement(523865..524716) Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange YKR047W 1 524899 525204 OFF 11 YKR047W (524899..525204) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 YKR048C 0 525029 526282 ON 11 NAP1 complement(525029..526282) Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus YKR049C 0 526472 526873 ON 11 FMP46 complement(526472..526873) Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR050W 1 527457 530126 ON 11 TRK2 (527457..530126) Component of the Trk1p-Trk2p potassium transport system YKR051W 1 530588 531844 ON 11 YKR051W (530588..531844) Putative protein of unknown function YKR052C 0 532192 533106 ON 11 MRS4 complement(532192..533106) Mitochondrial iron transporter of the mitochondrial carrier family (MCF), very similar to and functionally redundant with Mrs3p; functions under low-iron conditions; may transport other cations in addition to iron YKR053C 0 533709 534923 ON 11 YSR3 complement(533709..534923) Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p YKR054C 0 535289 547567 ON 11 DYN1 complement(535289..547567) Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p YKR055W 1 547858 548733 ON 11 RHO4 (547858..548733) Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity YKR056W 1 549090 551009 ON 11 TRM2 (549090..551009) tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair YKR057W 1 551299 551884 ON 11 RPS21A (551299..551322,551645..551884) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps21Bp and has similarity to rat S21 ribosomal protein YKR058W 1 552412 554262 ON 11 GLG1 (552412..554262) Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin YKR059W 1 554629 555816 ON 11 TIF1 (554629..555816) Translation initiation factor eIF4A, identical to Tif2p; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G YKR060W 1 556160 556984 ON 11 UTP30 (556160..556984) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YKR061W 1 557319 558596 ON 11 KTR2 (557319..558596) Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family YKR062W 1 559308 560294 ON 11 TFA2 (559308..560294) TFIIE small subunit, involved in RNA polymerase II transcription initiation YKR063C 0 560431 561939 ON 11 LAS1 complement(560431..561939) Essential nuclear protein possibly involved in bud formation and morphogenesis; mutants require the SSD1-v allele for viability YKR064W 1 562189 564780 ON 11 YKR064W (562189..564780) Zinc cluster protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR065C 0 564941 565534 ON 11 PAM17 complement(564941..565534) Presequence translocase-associated motor subunit, required for stable complex formation between cochaperones Pam16p and Pam18p, promotes association of Pam16p-Pam18p with the presequence translocase YKR066C 0 565755 566840 ON 11 CCP1 complement(565755..566840) Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress YKR067W 1 567560 569791 ON 11 GPT2 (567560..569791) Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis YKR068C 0 569970 570551 ON 11 BET3 complement(569970..570551) Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex YKR069W 1 571254 573035 ON 11 MET1 (571254..573035) S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis YKR070W 1 573216 574274 ON 11 YKR070W (573216..574274) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YKR071C 0 574576 575622 ON 11 DRE2 complement(574576..575622) Protein of unknown function; mutation displays synthetic lethal interaction with the pol3-13 allele of CDC2 YKR072C 0 576077 577765 ON 11 SIS2 complement(576077..577765) Negative regulatory subunit of the protein phosphatase 1 Ppz1p; involved in ion homeostasis and cell cycle progression YKR073C 0 577829 578149 OFF 11 YKR073C complement(577829..578149) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YKR074W 1 578851 579318 ON 11 YKR074W (578851..579318) Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm YKR075C 0 579469 580392 ON 11 YKR075C complement(579469..580392) Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS YKR075W-A 1 579854 580123 OFF 11 YKR075W-A (579854..580123) Dubious open reading frame unlikely to encode a protein; completely overlaps the uncharacterized gene YKR075W; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YKR076W 1 581925 583037 ON 11 ECM4 (581925..583037) Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm YKR077W 1 583295 584386 ON 11 YKR077W (583295..584386) Putative transcriptional activator, identified by high-throughput two-hybrid experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YKR077W is not an essential gene YKR078W 1 584594 586351 ON 11 YKR078W (584594..586351) Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) YKR079C 0 586431 588947 ON 11 TRZ1 complement(586431..588947) tRNase Z, involved in RNA processing, has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif, homolog of the human candidate prostate cancer susceptibility gene ELAC2 YKR080W 1 590037 590999 ON 11 MTD1 (590037..590999) NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline YKR081C 0 591148 592182 ON 11 RPF2 complement(591148..592182) Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles YKR082W 1 592467 595940 ON 11 NUP133 (592467..595940) Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p YKR083C 0 596063 596464 ON 11 DAD2 complement(596063..596464) Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis YKR084C 0 596697 598532 ON 11 HBS1 complement(596697..598532) GTP binding protein with sequence similarity to the elongation factor class of G proteins, EF-1alpha and Sup35p; associates with Dom34p, and shares a similar genetic relationship with genes that encode ribosomal protein components YKR085C 0 598736 599323 ON 11 MRPL20 complement(598736..599323) Mitochondrial ribosomal protein of the large subunit YKR086W 1 599499 602714 ON 11 PRP16 (599499..602714) RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity YKR087C 0 602837 603874 ON 11 OMA1 complement(602837..603874) Metalloendopeptidase of the mitochondrial inner membrane, involved in turnover of membrane-embedded proteins; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes YKR088C 0 604047 605060 ON 11 TVP38 complement(604047..605060) Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YKR089C 0 605275 608007 ON 11 TGL4 complement(605275..608007) Triacylglycerol lipase involved in triacylglycerol mobilization and degradation; found in lipid particles; potential Cdc28p substrate YKR090W 1 608588 610708 ON 11 PXL1 (608588..610708) LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin YKR091W 1 611167 611907 ON 11 SRL3 (611167..611907) Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate YKR092C 0 612307 613527 ON 11 SRP40 complement(612307..613527) Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 YKR093W 1 615372 617177 ON 11 PTR2 (615372..617177) Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p YKR094C 0 617638 618392 ON 11 RPL40B complement(617638..618016,618385..618392) Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes YKR095W 1 619447 625074 ON 11 MLP1 (619447..625074) Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs YKR095W-A 1 625506 625847 ON 11 PCC1 (625506..625543,625619..625847) Proposed transcription factor involved in the expression of genes regulated by alpha-factor and galactose; component of the EKC/KEOPS protein complex with Kae1p, Gon7p, Bud32p, and Cgi121p; related to human cancer-testis antigens YKR096W 1 626435 630022 ON 11 YKR096W (626435..630022) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc (PilT N terminus) domain YKR097W 1 630790 632439 ON 11 PCK1 (630790..632439) Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol YKR098C 0 632664 634817 ON 11 UBP11 complement(632664..634817) Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins YKR099W 1 635489 637924 ON 11 BAS1 (635489..637924) Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes YKR100C 0 638539 639606 ON 11 SKG1 complement(638539..639606) Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell YKR101W 1 640106 642142 ON 11 SIR1 (640106..642142) Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin YKR102W 1 645994 649503 ON 11 FLO10 (645994..649503) Lectin-like protein with similarity to Flo1p, thought to be involved in flocculation YKR103W 1 652718 656374 ON 11 NFT1 (652718..656374) Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. YKR104W 1 656474 657394 ON 11 YKR104W (656474..657394) Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds YKR105C 0 658354 660102 ON 11 YKR105C complement(658354..660102) Putative transporter of the Major Facilitator Superfamily (MFS) YKR106W 1 661080 662927 ON 11 YKR106W (661080..662927) Protein of unconfirmed function; displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins; coding sequence 98% identical to that of YCL073C YLL067C 0 585 4301 ON 12 YLL067C complement(585..3915,4015..4301) Putative protein of unknown function with similarity to helicases YLL067W-A 1 843 1325 OFF 12 YLL067W-A (843..1325) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps YLL067C YLL066W-B 1 5605 5775 OFF 12 YLL066W-B (5605..5775) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLL066C 0 6120 9836 ON 12 YLL066C complement(6120..9450,9550..9836) Putative protein of unknown function with similarity to helicases; YLL066C is not an essential gene YLL066W-A 1 6486 6968 OFF 12 YLL066W-A (6486..6968) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YLL066C YLL065W 1 11726 12076 OFF 12 YLL065W (11726..12076) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps chromosome XII telomeric element core sequences YLL064C 0 13083 13445 ON 12 YLL064C complement(13083..13445) Hypothetical protein YLL063C 0 14648 16072 ON 12 AYT1 complement(14648..16072) Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis YLL062C 0 16639 17613 ON 12 MHT1 complement(16639..17613) S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio YLL061W 1 17956 19707 ON 12 MMP1 (17956..19707) High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p YLL060C 0 21138 21839 ON 12 GTT2 complement(21138..21839) Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p YLL059C 0 22474 22980 OFF 12 YLL059C complement(22474..22980) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLL058W 1 23569 25296 ON 12 YLL058W (23569..25296) Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene YLL057C 0 25756 26994 ON 12 JLP1 complement(25756..26994) Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) YLL056C 0 27409 28305 ON 12 YLL056C complement(27409..28305) Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in pleiotropic drug resistance (PDR) phenomenon; YLL056C is not an essential gene YLL055W 1 30109 31704 ON 12 YCT1 (30109..31704) High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene YLL054C 0 32894 35203 ON 12 YLL054C complement(32894..35203) Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene YLL053C 0 35502 35960 OFF 12 YLL053C complement(35502..35960) Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin YLL052C 0 35911 36360 ON 12 AQY2 complement(35911..36360) Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains YLL051C 0 37332 39470 ON 12 FRE6 complement(37332..39470) Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels YLL050C 0 39803 40413 ON 12 COF1 complement(39803..40220,40400..40413) Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments , thought to be regulated by phosphorylation at SER4, ubiquitous and essential in eukaryotes YLL049W 1 40665 41204 ON 12 LDB18 (40665..41204) Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface YLL048C 0 41279 46264 ON 12 YBT1 complement(41279..46264) Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters YLL047W 1 46671 47054 OFF 12 YLL047W (46671..47054) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 YLL046C 0 46713 47462 ON 12 RNP1 complement(46713..47462) Ribonucleoprotein that contains two RNA recognition motifs (RRM) YLL045C 0 47858 48628 ON 12 RPL8B complement(47858..48628) Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits YLL044W 1 48215 48661 OFF 12 YLL044W (48215..48661) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant YLL043W 1 49937 51946 ON 12 FPS1 (49937..51946) Plasma membrane glycerol channel, member of the major intrinsic protein (MIP) family of channel proteins; involved in efflux of glycerol and in uptake of the trivalent metalloids arsenite and antimonite YLL042C 0 52086 52589 ON 12 ATG10 complement(52086..52589) E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy YLL041C 0 53130 53930 ON 12 SDH2 complement(53130..53930) Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone YLL040C 0 54210 63644 ON 12 VPS13 complement(54210..63644) Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention YLL039C 0 64061 65206 ON 12 UBI4 complement(64061..65206) Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats YLL038C 0 65774 66517 ON 12 ENT4 complement(65774..66517) Protein of unknown function, contains an N-terminal epsin-like domain YLL037W 1 66561 66941 OFF 12 YLL037W (66561..66941) Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor YLL036C 0 66744 68255 ON 12 PRP19 complement(66744..68255) Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases YLL035W 1 68579 70477 ON 12 GRC3 (68579..70477) Protein of unknown function, required for cell growth and possibly involved in rRNA processing; mRNA is cell cycle regulated YLL034C 0 70632 73145 ON 12 RIX7 complement(70632..73145) Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions YLL033W 1 73408 74100 ON 12 YLL033W (73408..74100) Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52 foci YLL032C 0 74269 76746 ON 12 YLL032C complement(74269..76746) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene YLL031C 0 77151 80204 ON 12 GPI13 complement(77151..80204) ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein YLL030C 0 80355 80696 OFF 12 YLL030C complement(80355..80696) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLL029W 1 81460 83709 ON 12 YLL029W (81460..83709) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL029W is not an essential gene but mutant is defective in spore formation YLL028W 1 84803 86563 ON 12 TPO1 (84803..86563) Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane YLL027W 1 87402 88154 ON 12 ISA1 (87402..88154) Mitochondrial matrix protein involved in biogenesis of the iron-sulfur (Fe/S) cluster of Fe/S proteins, isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources YLL026W 1 88622 91348 ON 12 HSP104 (88622..91348) Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation YLL025W 1 94746 95120 ON 12 YLL025W (94746..95120) Putative protein of unknown function; YLL025W is not an essential gene YLL024C 0 95565 97484 ON 12 SSA2 complement(95565..97484) ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall YLL023C 0 97996 98835 ON 12 YLL023C complement(97996..98835) Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum YLL022C 0 99043 100200 ON 12 HIF1 complement(99043..100200) Non-essential component of the HAT-B histone acetyltransferase complex (Hat1p-Hat2p-Hif1p), localized to the nucleus; has a role in telomeric silencing YLL021W 1 100946 105346 ON 12 SPA2 (100946..105346) Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate YLL020C 0 105555 105860 OFF 12 YLL020C complement(105555..105860) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 YLL019C 0 105685 107898 ON 12 KNS1 complement(105685..107898) Nonessential putative protein kinase of unknown cellular role; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues YLL019W-A 1 106907 106999 OFF 12 YLL019W-A (106907..106999) Dubious open reading frame unlikely to encode a protein; completely overlaps the verified gene KNS1 YLL018C-A 0 108675 108971 ON 12 COX19 complement(108675..108971) Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase YLL018C 0 109901 111574 ON 12 DPS1 complement(109901..111574) Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation YLL017W 1 112233 112544 OFF 12 YLL017W (112233..112544) Non-essential Ras guanine nucleotide exchange factor (GEF) localized to the membrane; expressed in poor nutrients and on non-fermentable carbon sources; homologous to CDC25; contains a stop codon in S288C; full-length gene includes YLL016W YLL016W 1 112846 115992 ON 12 SDC25 (112846..115992) Non-essential Ras guanine nucleotide exchange factor (GEF) localized to the membrane; expressed in poor nutrients and on non-fermentable carbon sources; homologous to CDC25; contains a stop codon in S288C; full-length gene includes YLL017W YLL015W 1 116431 121110 ON 12 BPT1 (116431..121110) ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p YLL014W 1 121321 121647 ON 12 YLL014W (121321..121647) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLL014W is not an essential gene YLL013C 0 122074 124713 ON 12 PUF3 complement(122074..124713) Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins YLL012W 1 125533 127254 ON 12 YEH1 (125533..127254) Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes YLL011W 1 127522 128991 ON 12 SOF1 (127522..128991) Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p YLL010C 0 129329 130612 ON 12 PSR1 complement(129329..130612) Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p YLL009C 0 131204 131413 ON 12 COX17 complement(131204..131413) Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase YLL008W 1 131728 133986 ON 12 DRS1 (131728..133986) Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles YLL007C 0 134301 136298 ON 12 YLL007C complement(134301..136298) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene YLL006W-A 1 136344 136520 OFF 12 YLL006W-A (136344..136520) Putative protein of unknown function; identified by fungal homology and RT-PCR YLL006W 1 136585 137865 ON 12 MMM1 (136585..137865) Mitochondrial outer membrane protein, component of the Mdm10-Mdm12-Mmm1 complex involved in import and assembly of outer membrane beta-barrel proteins YLL005C 0 137939 140545 ON 12 SPO75 complement(137939..140545) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis YLL004W 1 141072 142922 ON 12 ORC3 (141072..142922) Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing YLL003W 1 143200 146040 ON 12 SFI1 (143200..146040) Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C YLL002W 1 146290 147600 ON 12 RTT109 (146290..147600) Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase, acetylates H3-K56; plays a role in regulation of Ty1 transposition YLL001W 1 147889 150162 ON 12 DNM1 (147889..150162) Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis YLR001C 0 151388 153976 ON 12 YLR001C complement(151388..153976) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR001C is not an essential gene YLR002C 0 154342 156333 ON 12 NOC3 complement(154342..156333) Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation YLR003C 0 156854 157729 ON 12 YLR003C complement(156854..157729) Putative protein of unknown function that may participate in DNA replication; green fluorescent protein (GFP)-fusion protein is localized to the nucleus; YLR003C is not an essential gene YLR004C 0 157933 159504 ON 12 THI73 complement(157933..159504) Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs YLR005W 1 160048 161433 ON 12 SSL1 (160048..161433) Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p YLR006C 0 161754 163892 ON 12 SSK1 complement(161754..163892) Cytoplasmic response regulator, part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate YLR007W 1 164391 165401 ON 12 NSE1 (164391..165401) Essential subunit of the Mms21-Smc5-Smc6 complex; nuclear protein required for DNA repair and growth YLR008C 0 165576 166082 ON 12 PAM18 complement(165576..166082) J-protein co-chaperone of the mitochondrial import motor associated with the presequence translocase, with Ssc1p, Tim44p, Mge1p, and Pam16p; stimulates ATPase activity of Ssc1p to drive mitochondrial import; activity is inhibited by Pam16p YLR009W 1 166536 167135 ON 12 RLP24 (166536..167135) Essential protein with similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S ribosomal subunits and required for ribosomal large subunit biogenesis YLR010C 0 167319 167801 ON 12 TEN1 complement(167319..167801) Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p YLR011W 1 169102 169677 ON 12 LOT6 (169102..169677) FMN-dependent NAD(P)H:quinone reductase that may be involved in quinone detoxification; gene expression increases in cultures shifted to a lower temperature YLR012C 0 169981 170280 ON 12 YLR012C complement(169981..170280) Putative protein of unknown function; YLR012C is not an essential gene YLR013W 1 171338 171763 ON 12 GAT3 (171338..171763) Protein containing GATA family zinc finger motifs YLR014C 0 172267 174981 ON 12 PPR1 complement(172267..174981) Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p YLR015W 1 175226 176743 ON 12 BRE2 (175226..176743) Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L YLR016C 0 176801 177415 ON 12 PML1 complement(176801..177415) Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p YLR017W 1 177607 178620 ON 12 MEU1 (177607..178620) Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression YLR018C 0 178706 179605 ON 12 POM34 complement(178706..179605) Integral membrane protein of the nuclear pore; has an important role in maintaining the architecture of the pore complex YLR019W 1 180287 181480 ON 12 PSR2 (180287..181480) Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p YLR020C 0 181788 183404 ON 12 YEH2 complement(181788..183404) Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism YLR021W 1 183622 184161 ON 12 YLR021W (183622..184161) Putative protein of unknown function; mutant accumulates unusually high amounts of dityrosine in the soluble fraction though spores appear wild-type; YLR021W is not an essential gene; null mutant displays increased levels of spontaneous Rad52 foci YLR022C 0 184173 184925 ON 12 SDO1 complement(184173..184925) Essential protein involved in 60S ribosome maturation; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archae and eukaryotes YLR023C 0 185497 187128 ON 12 IZH3 complement(185497..187128) Membrane protein involved in zinc metabolism, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity YLR024C 0 187664 193282 ON 12 UBR2 complement(187664..193282) Cytoplasmic ubiquitin-protein ligase (E3) YLR025W 1 194453 195175 ON 12 SNF7 (194453..195175) One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes YLR026C 0 195451 196473 ON 12 SED5 complement(195451..196473) cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins YLR027C 0 196830 198086 ON 12 AAT2 complement(196830..198086) Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells YLR028C 0 199541 201316 ON 12 ADE16 complement(199541..201316) Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine YLR029C 0 201977 202591 ON 12 RPL15A complement(201977..202591) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA YLR030W 1 203291 204082 OFF 12 YLR030W (203291..204082) Putative protein of unknown function YLR031W 1 204226 204786 ON 12 YLR031W (204226..204786) Putative protein of unknown function YLR032W 1 204992 208501 ON 12 RAD5 (204992..208501) Single-stranded DNA-dependent ATPase, involved in postreplication repair; contains RING finger domain YLR033W 1 208762 210270 ON 12 RSC58 (208762..210270) Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance58KDa subunit YLR034C 0 210513 211934 ON 12 SMF3 complement(210513..211934) Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins YLR035C 0 212370 214457 ON 12 MLH2 complement(212370..214457) Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p YLR036C 0 221522 222133 ON 12 YLR036C complement(221522..222133) Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein YLR037C 0 222686 223060 ON 12 DAN2 complement(222686..223060) Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth YLR038C 0 224922 225173 ON 12 COX12 complement(224922..225173) Subunit VIb of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of fully active cytochrome c oxidase but not required for activity after assembly YLR039C 0 225427 228597 ON 12 RIC1 complement(225427..228597) Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes YLR040C 0 228909 229583 ON 12 YLR040C complement(228909..229583) Putative protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; YLR040C is not essential YLR041W 1 229378 229698 OFF 12 YLR041W (229378..229698) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C YLR042C 0 229967 230452 ON 12 YLR042C complement(229967..230452) Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene YLR043C 0 231703 232014 ON 12 TRX1 complement(231703..232014) Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance YLR044C 0 232391 234082 ON 12 PDC1 complement(232391..234082) Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism YLR045C 0 235038 237704 ON 12 STU2 complement(235038..237704) Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p YLR046C 0 238002 238814 ON 12 YLR046C complement(238002..238814) Putative membrane protein, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance YLR047C 0 239348 241408 ON 12 FRE8 complement(239348..241408) Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p YLR048W 1 242233 243350 ON 12 RPS0B (242233..242322,242682..243350) Protein component of the small (40S) ribosomal subunit, nearly identical to Rps0Ap; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal YLR049C 0 243887 245173 ON 12 YLR049C complement(243887..245173) Putative protein of unknown function YLR050C 0 245588 246073 ON 12 YLR050C complement(245588..246073) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene YLR051C 0 246325 246978 ON 12 FCF2 complement(246325..246978) Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon YLR052W 1 247202 247954 ON 12 IES3 (247202..247954) Subunit of the INO80 chromatin remodeling complex YLR053C 0 248101 248427 ON 12 YLR053C complement(248101..248427) Putative protein of unknown function YLR054C 0 248718 250979 ON 12 OSW2 complement(248718..250861,250949..250979) Protein of unknown function proposed to be involved in the assembly of the spore wall YLR055C 0 251273 253081 ON 12 SPT8 complement(251273..253081) Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters YLR056W 1 253862 254959 ON 12 ERG3 (253862..254959) C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources YLR057W 1 255307 257856 ON 12 YLR057W (255307..257856) Putative protein of unknown function; YLR050C is not an essential gene YLR058C 0 257993 259402 ON 12 SHM2 complement(257993..259402) Cytosolic serine hydroxymethyltransferase, involved in one-carbon metabolism YLR059C 0 259739 260548 ON 12 REX2 complement(259739..260548) RNA exonuclease, required for U4 snRNA maturation; functions redundantly with Rnh70p in 5.8S rRNA maturation, and with Rnh70p and Rex3p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases YLR060W 1 260980 262767 ON 12 FRS1 (260980..262767) Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; sequence is evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase (Msf1p), but substrate binding is similar YLR061W 1 263195 263949 ON 12 RPL22A (263195..263206,263596..263949) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 ribosomal protein YLR062C 0 263578 263955 OFF 12 BUD28 complement(263578..263955) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay YLR063W 1 264158 265255 ON 12 YLR063W (264158..265255) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene YLR064W 1 265457 266278 ON 12 YLR064W (265457..266278) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR064W is not an essential gene YLR065C 0 266374 266919 ON 12 YLR065C complement(266374..266919) Putative protein of unknown function; YLR065C is not an essential gene YLR066W 1 267170 267724 ON 12 SPC3 (267170..267724) Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 YLR067C 0 267814 270711 ON 12 PET309 complement(267814..270711) Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane YLR068W 1 271009 271464 ON 12 FYV7 (271009..271464) Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in processing the 35S rRNA primary transcript to generate the 20S and 27SA2 pre-rRNA transcripts YLR069C 0 271631 273916 ON 12 MEF1 complement(271631..273916) Mitochondrial elongation factor involved in translational elongation YLR070C 0 274142 275212 ON 12 XYL2 complement(274142..275212) Xylitol dehydrogenase, converts xylitol to D-xylulose in the endogenous xylose utilization pathway YLR071C 0 275379 278627 ON 12 RGR1 complement(275379..278627) Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation YLR072W 1 278863 280944 ON 12 YLR072W (278863..280944) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene YLR073C 0 281020 281622 ON 12 YLR073C complement(281020..281622) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to endosomes; YLR073C is not an esssential gene YLR074C 0 281957 282457 ON 12 BUD20 complement(281957..282457) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YLR075W 1 282928 283593 ON 12 RPL10 (282928..283593) Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family YLR076C 0 283186 283608 OFF 12 YLR076C complement(283186..283608) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 YLR077W 1 283873 285624 ON 12 YLR077W (283873..285624) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR078C 0 285737 286560 ON 12 BOS1 complement(285737..286468,286558..286560) v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi YLR079W 1 286821 287675 ON 12 SIC1 (286821..287675) Inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 YLR080W 1 287917 289251 ON 12 EMP46 (287917..289251) Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport YLR081W 1 290213 291937 ON 12 GAL2 (290213..291937) Galactose permease, required for utilization of galactose; also able to transport glucose YLR082C 0 292395 293573 ON 12 SRL2 complement(292395..293573) Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation YLR083C 0 294092 296095 ON 12 EMP70 complement(294092..296095) Protein whose 24kDa cleavage product is found in endosome-enriched membrane fractions, predicted to be a transmembrane protein YLR084C 0 296590 300252 ON 12 RAX2 complement(296590..300252) N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p YLR085C 0 300674 301990 ON 12 ARP6 complement(300674..301990) Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A YLR086W 1 302244 306500 ON 12 SMC4 (302244..306500) Subunit of the condensin complex, which reorganizes chromosomes during cell division, forms a stable complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity and promotes knotting of circular DNA; potential Cdc28p substrate YLR087C 0 306856 315732 ON 12 CSF1 complement(306856..315732) Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR088W 1 316108 317952 ON 12 GAA1 (316108..317952) Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER YLR089C 0 318238 320016 ON 12 YLR089C complement(318238..320016) Putative alanine transaminase (glutamic pyruvic transaminase); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR090W 1 320702 322081 ON 12 XDJ1 (320702..322081) Putative chaperone, homolog of E. coli DnaJ, closely related to Ydj1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR091W 1 322298 323179 ON 12 YLR091W (322298..323179) Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR091W is not an esssential gene YLR092W 1 323545 326226 ON 12 SUL2 (323545..326226) High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates YLR093C 0 326514 327416 ON 12 NYV1 complement(326514..327259,327401..327416) v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane YLR094C 0 327731 329239 ON 12 GIS3 complement(327731..329239) Protein of unknown function YLR095C 0 329678 332116 ON 12 IOC2 complement(329678..332116) Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif YLR096W 1 332591 336034 ON 12 KIN2 (332591..336034) Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p YLR097C 0 336232 337266 ON 12 HRT3 complement(336232..337266) Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies YLR098C 0 337528 339474 ON 12 CHA4 complement(337528..339474) DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain YLR099C 0 339745 340929 ON 12 ICT1 complement(339745..340929) Protein of unknown function, null mutation leads to an increase in sensitivity to Calcofluor white; expression of the gene is induced in the presence of isooctane YLR099W-A 1 341326 341589 ON 12 YLR099W-A (341326..341589) Putative protein of unknown function YLR100W 1 341811 342854 ON 12 ERG27 (341811..342854) 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs YLR101C 0 342567 342962 OFF 12 YLR101C complement(342567..342962) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W YLR102C 0 342971 343768 ON 12 APC9 complement(342971..343768) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition YLR103C 0 343990 345942 ON 12 CDC45 complement(343990..345942) DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes YLR104W 1 346586 346981 ON 12 YLR104W (346586..346981) Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate YLR105C 0 347048 348181 ON 12 SEN2 complement(347048..348181) Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease YLR106C 0 349007 363739 ON 12 MDN1 complement(349007..363739) Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm YLR107W 1 364117 365331 ON 12 REX3 (364117..365331) RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases YLR108C 0 366668 368125 ON 12 YLR108C complement(366668..368125) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene YLR109W 1 368782 369312 ON 12 AHP1 (368782..369312) Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p YLR110C 0 369698 370099 ON 12 CCW12 complement(369698..370099) Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor YLR111W 1 370392 370724 OFF 12 YLR111W (370392..370724) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR112W 1 370792 371211 OFF 12 YLR112W (370792..371211) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR113W 1 371621 372928 ON 12 HOG1 (371621..372928) Mitogen-activated protein kinase involved in osmoregulation via three independent osmosensors; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p YLR114C 0 374945 377239 ON 12 AVL9 complement(374945..377239) Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms YLR115W 1 377986 380565 ON 12 CFT2 (377986..380565) Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. YLR116W 1 380823 382253 ON 12 MSL5 (380823..382253) Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA YLR117C 0 382472 384535 ON 12 CLF1 complement(382472..384535) Essential splicesome assembly factor; contains multiple tetratricopeptide repeat (TPR) protein-binding motifs and interacts specifically with many spliceosome components, may serve as a scaffold during splicesome assembly YLR118C 0 384726 385409 ON 12 YLR118C complement(384726..385409) Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS YLR119W 1 385535 386176 ON 12 SRN2 (385535..386176) Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus YLR120C 0 386512 388221 ON 12 YPS1 complement(386512..388221) Aspartic protease, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor YLR120W-A 1 388673 388774 OFF 12 YLR120W-A (388673..388774) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ORF YLR121C YLR121C 0 388745 390271 ON 12 YPS3 complement(388745..390271) Aspartic protease, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor YLR122C 0 390955 391332 OFF 12 YLR122C complement(390955..391332) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C YLR123C 0 391079 391408 OFF 12 YLR123C complement(391079..391408) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif YLR124W 1 391601 391945 OFF 12 YLR124W (391601..391945) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR125W 1 393485 393895 ON 12 YLR125W (393485..393895) Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene YLR126C 0 394766 395521 ON 12 YLR126C complement(394766..395521) Putative protein of unknown function with similarity to glutamine amidotransferase proteins; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein YLR127C 0 395759 398320 ON 12 APC2 complement(395759..398320) Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p YLR128W 1 398531 399434 ON 12 DCN1 (398531..398533,398628..399434) Putative Nedd8 ligase; binds Nedd8; involved in cullin neddylation; not essential; similar to C.elegans DCN-1; contains UBA-like ubiquitin-binding domain and a DUF298 domain YLR129W 1 399658 402489 ON 12 DIP2 (399658..402489) Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex YLR130C 0 402795 404063 ON 12 ZRT2 complement(402795..404063) Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor YLR131C 0 404511 406823 ON 12 ACE2 complement(404511..406823) Transcription factor that activates expression of early G1-specific genes, localizes to daughter cell nuclei after cytokinesis and delays G1 progression in daughters, localization is regulated by phosphorylation; potential Cdc28p substrate YLR132C 0 407284 408156 ON 12 YLR132C complement(407284..408156) Protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to mitochondria and nucleus; YLR132C is an essential gene YLR133W 1 408446 410194 ON 12 CKI1 (408446..410194) Choline kinase, catalyzes the first step in the CDP-choline pathway phosphatidylcholine synthesis (Kennedy pathway); mRNA expression is regulated by inositol and choline, enzyme activity is stimulated by phosphorylation by protein kinase YLR134W 1 410724 412415 ON 12 PDC5 (410724..412415) Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism YLR135W 1 413282 415528 ON 12 SLX4 (413282..415528) Subunit of a complex, with Slx1p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p YLR136C 0 415802 416659 ON 12 TIS11 complement(415802..416659) mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis YLR137W 1 417007 418110 ON 12 YLR137W (417007..418110) Putative protein of unknown function YLR138W 1 418438 421395 ON 12 NHA1 (418438..421395) Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH YLR139C 0 421543 423474 ON 12 SLS1 complement(421543..423474) Mitochondrial membrane protein required for assembly of respiratory-chain enzyme complexes III and IV; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery YLR140W 1 423475 423801 OFF 12 YLR140W (423475..423801) Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I YLR141W 1 423684 424775 ON 12 RRN5 (423684..424775) Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p YLR142W 1 425187 426617 ON 12 PUT1 (425187..426617) Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source YLR143W 1 427330 429387 ON 12 YLR143W (427330..429387) Putative protein of unknown function; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; YLR143W is not an essential gene YLR144C 0 429678 432017 ON 12 ACF2 complement(429678..432017) Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly YLR145W 1 432169 432774 ON 12 RMP1 (432169..432774) Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P YLR146C 0 432824 433726 ON 12 SPE4 complement(432824..433726) Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid YLR146W-A 1 433871 434059 ON 12 YLR146W-A (433871..434059) Putative protein of unknown function YLR147C 0 434159 434464 ON 12 SMD3 complement(434159..434464) Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 YLR148W 1 434642 437398 ON 12 PEP3 (434642..437398) Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis YLR149C 0 437632 439824 ON 12 YLR149C complement(437632..439824) Putative protein of unknown function; YLR149C is not an essential gene YLR149C-A 0 440371 440457 OFF 12 YLR149C-A complement(440371..440457) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR150W 1 440468 441289 ON 12 STM1 (440468..441289) Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations YLR151C 0 441716 442738 ON 12 PCD1 complement(441716..442738) Peroxisomal nudix pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids YLR152C 0 442959 444689 ON 12 YLR152C complement(442959..444689) Putative protein of unknown function; YLR152C is not an essential gene YLR153C 0 445525 447576 ON 12 ACS2 complement(445525..447576) Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions YLR154C 0 447983 448315 ON 12 RNH203 complement(447983..448315) Ribonuclease H2 subunit, required for RNase H2 activity YLR154W-A 1 452439 452624 OFF 12 YLR154W-A (452439..452624) Dubious open reading frame unlikely to encode a protein; encoded within the the 25S rRNA gene on the opposite strand YLR154W-B 1 454393 454557 OFF 12 YLR154W-B (454393..454557) Dubious open reading frame unlikely to encode a protein; encoded within the the 25S rRNA gene on the opposite strand YLR154W-C 1 454697 455071 OFF 12 TAR1 (454697..455071) Mitochondrial protein of unknown function, overexpression suppresses an rpo41 mutation affecting mitochondrial RNA polymerase; encoded within the 25S rRNA gene on the opposite strand YLR154W-E 1 455434 455637 OFF 12 YLR154W-E (455434..455637) Dubious open reading frame unlikely to encode a protein; encoded within the the 35S rRNA gene on the opposite strand YLR154W-F 1 455884 456024 OFF 12 YLR154W-F (455884..456024) Dubious open reading frame unlikely to encode a protein; encoded within the the 35S rRNA gene on the opposite strand YLR154C-G 0 462523 462672 OFF 12 YLR154C-G complement(462523..462672) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2 YLR154C-H 0 468828 468959 OFF 12 YLR154C-H complement(468828..468959) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-2 YLR155C 0 469318 470406 ON 12 ASP3-1 complement(469318..470406) Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C YLR156W 1 472114 472458 OFF 12 YLR156W (472114..472458) Putative protein of unknown function; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis YLR156C-A 0 472480 472611 OFF 12 YLR156C-A complement(472480..472611) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-3 YLR157C 0 472970 474058 ON 12 ASP3-2 complement(472970..474058) Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C YLR157W-D 1 475765 475977 OFF 12 YLR157W-D (475765..475977) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YLR157W-E 1 481874 482038 OFF 12 YLR157W-E (481874..482038) Putative protein of unknown function identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element YLR157C-C 0 482060 482191 OFF 12 YLR157C-C complement(482060..482191) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-4 YLR158C 0 482550 483638 ON 12 ASP3-3 complement(482550..483638) Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C YLR159W 1 485346 485690 OFF 12 YLR159W (485346..485690) Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 YLR159C-A 0 485712 485843 OFF 12 YLR159C-A complement(485712..485843) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RND5-5 YLR160C 0 486202 487290 ON 12 ASP3-4 complement(486202..487290) Cell-wall L-asparaginase II, involved in asparagine catabolism; expression is induced during nitrogen starvation; four copies of ASP3 are present in the genome reference strain S288C YLR161W 1 488998 489342 OFF 12 YLR161W (488998..489342) Putative protein of unknown function; YLR156W, YLR159W, and YLR161W are three identical open reading frames encoded near the ribosomal DNA region of chromosome 12 YLR162W 1 489574 489930 OFF 12 YLR162W (489574..489930) Putative protein of unknown function; overexpression confers resistance to the antimicrobial peptide MiAMP1 YLR162W-A 1 490407 490595 OFF 12 YLR162W-A (490407..490595) Putative protein of unknown function identified by fungal homology comparisons and RT-PCR YLR163C 0 491868 493256 ON 12 MAS1 complement(491868..493256) Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins YLR163W-A 1 492814 492927 OFF 12 YLR163W-A (492814..492927) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data YLR164W 1 493885 494391 ON 12 YLR164W (493885..494391) Mitochondrial inner membrane of unknown function; similar to Tim18p and Sdh4p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner YLR165C 0 494496 495260 ON 12 PUS5 complement(494496..495260) Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability YLR166C 0 495431 498046 ON 12 SEC10 complement(495431..498046) Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis YLR167W 1 498949 499407 ON 12 RPS31 (498949..499407) Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B YLR168C 0 499580 500272 ON 12 YLR168C complement(499580..500272) Putative protein of unknown function that may be involved in intramitochondrial sorting; has similarity to Ups1p and to human PRELI; the green fluorescent protein (GFP)-tagged protein localizes to mitochondria YLR169W 1 500337 500690 OFF 12 YLR169W (500337..500690) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR170C 0 500581 501051 ON 12 APS1 complement(500581..501051) Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex YLR171W 1 500735 501124 OFF 12 YLR171W (500735..501124) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR172C 0 501262 502164 ON 12 DPH5 complement(501262..502164) Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm YLR173W 1 502423 504249 ON 12 YLR173W (502423..504249) Putative protein of unknown function YLR174W 1 504593 505831 ON 12 IDP2 (504593..505831) Cytosolic NADP-specific isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose YLR175W 1 506136 507587 ON 12 CBF5 (506136..507587) Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2 YLR176C 0 507799 510234 ON 12 RFX1 complement(507799..510234) Protein involved in DNA damage and replication checkpoint pathway; recruits repressors Tup1p and Cyc8p to promoters of DNA damage-inducible genes; similar to a family of mammalian DNA binding RFX1-4 proteins YLR177W 1 511056 512942 ON 12 YLR177W (511056..512942) Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; YLR177W is not an essential gene YLR178C 0 513164 513823 ON 12 TFS1 complement(513164..513823) Carboxypeptidase Y inhibitor, function requires acetylation by the NatB N-terminal acetyltransferase; phosphatidylethanolamine-binding protein involved in protein kinase A signaling pathway YLR179C 0 514110 514715 ON 12 YLR179C complement(514110..514715) Protein of unknown function, transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus; YLR179C is not essential YLR180W 1 515264 516412 ON 12 SAM1 (515264..516412) S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p) YLR181C 0 516680 517672 ON 12 VTA1 complement(516680..517672) Multivesicular body (MVB) protein involved in endosomal protein sorting; binds to Vps20p and Vps4p; may regulate Vps4p function; bindsVps60p and may act at a late step in MVB formation; mutants show class E vacuolar-protein sorting defects YLR182W 1 517942 520353 ON 12 SWI6 (517942..520353) Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate YLR183C 0 520545 522014 ON 12 TOS4 complement(520545..522014) Transcription factor that binds to a number of promoter regions, particularly promoters of some genes involved in pheromone response and cell cycle; potential Cdc28p substrate; expression is induced in G1 by bound SBF YLR184W 1 522108 522455 OFF 12 YLR184W (522108..522455) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR185W 1 522665 523290 ON 12 RPL37A (522665..522671,523031..523290) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl37Bp and to rat L37 ribosomal protein YLR186W 1 523634 524392 ON 12 EMG1 (523634..524392) Protein required for the maturation of the 18S rRNA and for 40S ribosome production; associated with spindle/microtubules; nuclear localization depends on physical interaction with Nop14p; may bind snoRNAs YLR187W 1 524867 527947 ON 12 SKG3 (524867..527947) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p YLR188W 1 528302 530389 ON 12 MDL1 (528302..530389) Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress YLR189C 0 530799 534395 ON 12 ATG26 complement(530799..534395) UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids, involved in autophagy YLR190W 1 535216 536691 ON 12 MMR1 (535216..536691) Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p YLR191W 1 537274 538434 ON 12 PEX13 (537274..538434) Integral peroxisomal membrane required for the translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p YLR192C 0 538794 539591 ON 12 HCR1 complement(538794..539591) Dual function protein involved in translation initiation as a substoichiometric component of eukaryotic translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA YLR193C 0 540011 540538 ON 12 UPS1 complement(540011..540538) Mitochondrial intermembrane space protein that regulates alternative processing and sorting of Mgm1p and other proteins; required for normal mitochondrial morphology; ortholog of human PRELI YLR194C 0 540811 541575 ON 12 YLR194C complement(540811..541575) Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress YLR195C 0 541939 543306 ON 12 NMT1 complement(541939..543306) N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction YLR196W 1 543970 545700 ON 12 PWP1 (543970..545700) Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily YLR197W 1 546099 547613 ON 12 SIK1 (546099..547613) Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects YLR198C 0 547288 547647 OFF 12 YLR198C complement(547288..547647) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W YLR199C 0 547855 548771 ON 12 PBA1 complement(547855..548679,548766..548771) Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly YLR200W 1 549014 549358 ON 12 YKE2 (549014..549358) Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin YLR201C 0 549513 550295 ON 12 COQ9 complement(549513..550295) Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes YLR202C 0 550196 550638 OFF 12 YLR202C complement(550196..550460,550577..550638) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron YLR203C 0 550652 551962 ON 12 MSS51 complement(550652..551962) Nuclear encoded protein required for translation of COX1 mRNA; binds to Cox1 protein YLR204W 1 552272 552607 ON 12 QRI5 (552272..552607) Mitochondrial protein of unknown function YLR205C 0 552726 553679 ON 12 HMX1 complement(552726..553679) ER localized, heme-binding peroxidase involved in the degradation of heme; does not exhibit heme oxygenase activity despite similarity to heme oxygenases; expression regulated by AFT1 YLR206W 1 554580 556421 ON 12 ENT2 (554580..556421) Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus YLR207W 1 556790 559291 ON 12 HRD3 (556790..559291) Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events YLR208W 1 559553 560446 ON 12 SEC13 (559553..560446) Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles YLR209C 0 560799 561734 ON 12 PNP1 complement(560799..561734) Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides YLR210W 1 562010 563392 ON 12 CLB4 (562010..563392) B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation YLR211C 0 563794 564533 ON 12 YLR211C complement(563794..564456,564516..564533) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron YLR212C 0 564862 566283 ON 12 TUB4 complement(564862..566283) Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body YLR213C 0 566656 567924 ON 12 CRR1 complement(566656..567924) Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation YLR214W 1 568569 570629 ON 12 FRE1 (568569..570629) Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels YLR215C 0 570778 571860 ON 12 CDC123 complement(570778..571860) Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein YLR216C 0 572098 573213 ON 12 CPR6 complement(572098..573213) Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity YLR217W 1 572911 573234 OFF 12 YLR217W (572911..573234) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 YLR218C 0 573468 573920 ON 12 YLR218C complement(573468..573920) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR218C is not essential; mutants exhibit glycogen storage defects and growth defects on a non-fermentable carbon source YLR219W 1 574153 576339 ON 12 MSC3 (574153..576339) Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate YLR220W 1 576827 577795 ON 12 CCC1 (576827..577795) Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation YLR221C 0 578364 579026 ON 12 RSA3 complement(578364..579026) Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus YLR222C 0 579320 581773 ON 12 UTP13 complement(579320..581773) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YLR222C-A 0 582008 582238 OFF 12 YLR222C-A complement(582008..582238) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene IFH1 YLR223C 0 582235 585492 ON 12 IFH1 complement(582235..585492) Essential protein with a highly acidic N-terminal domain; IFH1 exhibits genetic interactions with FHL1, overexpression interferes with silencing at telomeres and HM loci; potential Cdc28p substrate YLR224W 1 586466 587575 ON 12 YLR224W (586466..587575) F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene YLR225C 0 587697 588920 ON 12 YLR225C complement(587697..588920) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene YLR226W 1 589356 590543 ON 12 BUR2 (589356..590543) Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II YLR227C 0 590564 592045 ON 12 ADY4 complement(590564..592045) Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane YLR228C 0 600021 602465 ON 12 ECM22 complement(600021..602465) Sterol regulatory element binding protein, regulates transcription of the sterol biosynthetic genes ERG2 and ERG3; member of the fungus-specific Zn[2]-Cys[6] binuclear cluster family of transcription factors; homologous to Upc2p YLR229C 0 604214 604789 ON 12 CDC42 complement(604214..604789) Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins YLR230W 1 604576 604881 OFF 12 YLR230W (604576..604881) Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity YLR231C 0 605760 607121 ON 12 BNA5 complement(605760..607121) Kynureninase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway YLR232W 1 606832 607179 OFF 12 YLR232W (606832..607179) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 YLR233C 0 607427 609526 ON 12 EST1 complement(607427..609526) TLC1 RNA-associated factor involved in telomere length regulation as the recruitment subunit of the telomerase holoenzyme, has a possible role in activating Est2p-TLC1-RNA bound to the telomere YLR234W 1 609785 611755 ON 12 TOP3 (609785..611755) DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination YLR235C 0 611534 611932 OFF 12 YLR235C complement(611534..611932) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TOP3 YLR236C 0 611796 612119 OFF 12 YLR236C complement(611796..612119) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR237W 1 612369 614165 ON 12 THI7 (612369..614165) Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia YLR238W 1 614757 616193 ON 12 FAR10 (614757..616193) Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate YLR239C 0 616334 617320 ON 12 LIP2 complement(616334..617320) Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups YLR240W 1 617535 620162 ON 12 VPS34 (617535..620162) Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p YLR241W 1 620475 622823 ON 12 YLR241W (620475..622823) Putative protein of unknown function, may be involved in detoxification YLR242C 0 622920 623885 ON 12 ARV1 complement(622920..623885) Protein required for normal intracellular sterol distribution and for sphingolipid metabolism; similar to Nup120p and C. elegans R05H5.5 protein YLR243W 1 624205 625023 ON 12 YLR243W (624205..625023) Putative protein of unknown function; YLR243W is an essential gene YLR244C 0 625170 626333 ON 12 MAP1 complement(625170..626333) Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p YLR245C 0 626504 626932 ON 12 CDD1 complement(626504..626932) Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm YLR246W 1 627120 628199 ON 12 ERF2 (627120..628199) Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole YLR247C 0 628686 633356 ON 12 YLR247C complement(628686..633356) Putative helicase; localized to mitochondria and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52 foci YLR248W 1 634254 636086 ON 12 RCK2 (634254..636086) Protein kinase involved in the response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations YLR249W 1 636782 639916 ON 12 YEF3 (636782..639916) Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP YLR250W 1 640319 641023 ON 12 SSP120 (640319..641023) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern YLR251W 1 641466 642059 ON 12 SYM1 (641466..642059) Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 YLR252W 1 641959 642264 OFF 12 YLR252W (641959..642264) Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism YLR253W 1 642629 644338 ON 12 YLR253W (642629..644338) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene YLR254C 0 644406 644975 ON 12 NDL1 complement(644406..644975) Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends YLR255C 0 645602 645955 OFF 12 YLR255C complement(645602..645955) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR256W 1 646417 650925 ON 12 HAP1 (646417..650925) Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus YLR257W 1 658828 659793 ON 12 YLR257W (658828..659793) Putative protein of unknown function YLR258W 1 660718 662835 ON 12 GSY2 (660718..662835) Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase YLR259C 0 663286 665004 ON 12 HSP60 complement(663286..665004) Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; similarity to groEL YLR260W 1 665846 667909 ON 12 LCB5 (665846..667909) Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules YLR261C 0 668239 668565 OFF 12 VPS63 complement(668239..668565) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect YLR262C 0 668246 668893 ON 12 YPT6 complement(668246..668893) GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 YLR262C-A 0 669470 669664 ON 12 TMA7 complement(669470..669664) Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin YLR263W 1 670342 672825 ON 12 RED1 (670342..672825) Protein component of the axial elements of the synaptonemal complex, involved in chromosome segregation during the first meiotic division; interacts with Hop1p; required for wild-type levels of Mek1p kinase activity YLR264W 1 673133 673336 ON 12 RPS28B (673133..673336) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps28Ap and has similarity to rat S28 ribosomal protein YLR264C-A 0 673830 673946 OFF 12 YLR264C-A complement(673830..673946) Putative protein of unknown function YLR265C 0 674429 675457 ON 12 NEJ1 complement(674429..675457) Protein involved in regulation of nonhomologous end joining; repressed by MAT heterozygosity; associates with Lif1p and regulates its cellular distribution YLR266C 0 675621 677726 ON 12 PDR8 complement(675621..677726) Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon YLR267W 1 678214 679926 ON 12 YLR267W (678214..679926) Protein of unknown function, overproduction suppresses a pam1 slv3 double null mutation YLR268W 1 680202 680846 ON 12 SEC22 (680202..680846) R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog YLR269C 0 680868 681218 OFF 12 YLR269C complement(680868..681218) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR270W 1 681188 682240 ON 12 DCS1 (681188..682240) Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p YLR271W 1 682739 683563 ON 12 YLR271W (682739..683563) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS YLR272C 0 683674 687204 ON 12 YCS4 complement(683674..687204) Non-SMC subunit of the condensin complex (Smc2p-Smc4p-Ycs4p-Brn1p-Ycg1p); required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin and silencing at the mating type locus YLR273C 0 689085 691031 ON 12 PIG1 complement(689085..691031) Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase YLR274W 1 691557 693884 ON 12 CDC46 (691557..693884) Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase YLR275W 1 694380 694802 ON 12 SMD2 (694380..694384,694475..694802) Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 YLR276C 0 695048 696832 ON 12 DBP9 complement(695048..696832) ATP-dependent RNA helicase of the DEAD-box family involved in biogenesis of the 60S ribosomal subunit YLR277C 0 697158 699497 ON 12 YSH1 complement(697158..699497) Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs YLR278C 0 700001 704026 ON 12 YLR278C complement(700001..704026) Zinc-cluster protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; YLR278C is not an essential gene YLR279W 1 704311 704700 OFF 12 YLR279W (704311..704700) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR280C 0 704485 704835 OFF 12 YLR280C complement(704485..704835) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR281C 0 704495 704962 ON 12 YLR281C complement(704495..704962) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene YLR282C 0 705073 705414 OFF 12 YLR282C complement(705073..705414) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR283W 1 705188 706132 ON 12 YLR283W (705188..706132) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene YLR284C 0 706200 707042 ON 12 ECI1 complement(706200..707042) Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced YLR285W 1 707362 708147 ON 12 NNT1 (707362..708147) Putative nicotinamide N-methyltransferase, has a role in rDNA silencing and in lifespan determination YLR285C-A 0 708170 708340 OFF 12 YLR285C-A complement(708170..708340) Putative protein of unknown function; identified by fungal homology and RT-PCR YLR286C 0 708450 710138 ON 12 CTS1 complement(708450..710138) Endochitinase, required for cell separation after mitosis; transcriptional activation during late G and early M cell cycle phases is mediated by transcription factor Ace2p YLR286W-A 1 708564 708698 OFF 12 YLR286W-A (708564..708698) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CTS1 YLR287C 0 710993 712060 ON 12 YLR287C complement(710993..712060) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene YLR287C-A 0 712539 713160 ON 12 RPS30A complement(712539..712727,713158..713160) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Bp and has similarity to rat S30 ribosomal protein YLR288C 0 713482 714906 ON 12 MEC3 complement(713482..714906) DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 YLR289W 1 715091 717028 ON 12 GUF1 (715091..717028) Mitochondrial GTPase of unknown function, similar to E. coli elongation factor-type GTP-binding protein LepA and to LK1236.1 from Caenorhabditis elegans YLR290C 0 717147 717980 ON 12 YLR290C complement(717147..717980) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene YLR291C 0 718319 719464 ON 12 GCD7 complement(718319..719464) Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression YLR292C 0 719791 720372 ON 12 SEC72 complement(719791..720372) Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YLR293C 0 720773 721432 ON 12 GSP1 complement(720773..721432) GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Prp20p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; yeast Gsp2p homolog YLR294C 0 721703 722032 OFF 12 YLR294C complement(721703..722032) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 YLR295C 0 722001 722375 ON 12 ATP14 complement(722001..722375) Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis YLR296W 1 722980 723306 OFF 12 YLR296W (722980..723306) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR297W 1 724046 724435 ON 12 YLR297W (724046..724435) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YLR297W is not an essential gene YLR298C 0 724723 725418 ON 12 YHC1 complement(724723..725418) Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site YLR299W 1 726071 728053 ON 12 ECM38 (726071..728053) Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; expression induced mainly by nitrogen starvation YLR299C-A 0 727913 728005 OFF 12 YLR299C-A complement(727913..728005) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ECM38 YLR300W 1 728957 730303 ON 12 EXG1 (728957..730303) Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes YLR301W 1 730827 731561 ON 12 YLR301W (730827..731561) Protein of unknown function that interacts with Sec72p YLR302C 0 732193 732555 OFF 12 YLR302C complement(732193..732555) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR303W 1 732544 733878 ON 12 MET17 (732544..733878) Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis YLR304C 0 735214 737550 ON 12 ACO1 complement(735214..737550) Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy YLR305C 0 738163 743865 ON 12 STT4 complement(738163..743865) Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization YLR306W 1 744153 744853 ON 12 UBC12 (744153..744155,744290..744853) Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes YLR307W 1 745622 746527 ON 12 CDA1 (745622..746527) Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall YLR307C-A 0 746850 747113 ON 12 YLR307C-A complement(746850..747113) Putative protein of unknown function YLR308W 1 747939 748877 ON 12 CDA2 (747939..748877) Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall YLR309C 0 749036 751771 ON 12 IMH1 complement(749036..751771) Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi YLR310C 0 752226 756995 ON 12 CDC25 complement(752226..756995) Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 YLR311C 0 757267 757614 OFF 12 YLR311C complement(757267..757614) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR312C 0 757639 758835 ON 12 YLR312C complement(757639..758835) Protein of unknown function; null mutant quiescent and non-quiescent cells exhibit reduced reproductive capacity YLR312W-A 1 759482 760243 ON 12 MRPL15 (759482..760243) Mitochondrial ribosomal protein of the large subunit YLR313C 0 760357 762342 ON 12 SPH1 complement(760357..762342) Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p YLR314C 0 762575 764137 ON 12 CDC3 complement(762575..764137) Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM YLR315W 1 764808 765269 ON 12 NKP2 (764808..765269) Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p) YLR316C 0 765266 766358 ON 12 TAD3 complement(765266..766073,766130..766181,766250..766358) Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs YLR317W 1 765655 766089 OFF 12 YLR317W (765655..766089) Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C YLR318W 1 766542 769196 ON 12 EST2 (766542..769196) Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia YLR319C 0 769318 771684 ON 12 BUD6 complement(769318..771684) Actin- and formin-interacting protein, involved in actin cable nucleation and polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate YLR320W 1 771940 776304 ON 12 MMS22 (771940..776304) Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks YLR321C 0 776584 777864 ON 12 SFH1 complement(776584..777864) Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog YLR322W 1 777628 777942 OFF 12 VPS65 (777628..777942) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect YLR323C 0 778173 778952 ON 12 CWC24 complement(778173..778952) Essential protein, component of a complex containing Cef1p; has similarity to S. pombe Cwf24p YLR324W 1 779215 780786 ON 12 PEX30 (779215..780786) Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p YLR325C 0 781143 781379 ON 12 RPL38 complement(781143..781379) Protein component of the large (60S) ribosomal subunit, has similarity to rat L38 ribosomal protein YLR326W 1 782174 782896 ON 12 YLR326W (782174..782896) Hypothetical protein YLR327C 0 783127 783387 ON 12 TMA10 complement(783127..783387) Protein of unknown function that associates with ribosomes YLR328W 1 784913 786118 ON 12 NMA1 (784913..786118) Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway YLR329W 1 786442 787333 ON 12 REC102 (786442..786615,786713..787333) Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination YLR330W 1 787664 789679 ON 12 CHS5 (787664..789679) Protein involved in export from the Golgi to plasma membrane; involved in chitin biosynthesis through its role in Chs3p localization; interacts with Arf1p, Bch1p, Fmp50p, Bud7p, and Chs6p YLR331C 0 790669 791046 OFF 12 JIP3 complement(790669..791046) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 YLR332W 1 790676 791806 ON 12 MID2 (790676..791806) O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p YLR333C 0 795573 795899 ON 12 RPS25B complement(795573..795899) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similarity to rat S25 ribosomal protein YLR334C 0 796698 797078 OFF 12 YLR334C complement(796698..797078) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here YLR335W 1 797430 799592 ON 12 NUP2 (797430..799592) Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization YLR336C 0 799697 802396 ON 12 SGD1 complement(799697..802396) Essential nuclear protein with a possible role in the osmoregulatory glycerol response; interacts with phospholipase C (Plc1p); putative homolog of human NOM1 which is implicated in acute myeloid leukemia YLR337C 0 802653 805106 ON 12 VRP1 complement(802653..805106) Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) YLR338W 1 804346 805203 OFF 12 OPI9 (804346..805203) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C YLR339C 0 805784 806335 OFF 12 YLR339C complement(805784..806335) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 YLR340W 1 805887 806825 ON 12 RPP0 (805887..806825) Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302 YLR341W 1 807385 808818 ON 12 SPO77 (807385..808818) Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis YLR342W 1 809997 815627 ON 12 FKS1 (809997..815627) Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling YLR342W-A 1 815810 815983 OFF 12 YLR342W-A (815810..815983) Putative protein of unknown function YLR343W 1 816094 817761 ON 12 GAS2 (816094..817761) 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p YLR344W 1 819312 820142 ON 12 RPL26A (819312..819330,819778..820142) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl26Bp and has similarity to E. coli L24 and rat L26 ribosomal proteins; binds to 5.8S rRNA YLR345W 1 820511 822040 ON 12 YLR345W (820511..822040) Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene YLR346C 0 822287 822592 ON 12 YLR346C complement(822287..822592) Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene YLR347C 0 823827 826412 ON 12 KAP95 complement(823827..826412) Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complex YLR347W-A 1 824788 824928 OFF 12 YLR347W-A (824788..824928) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF KAP95/YLR347C YLR348C 0 826976 827872 ON 12 DIC1 complement(826976..827872) Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix YLR349W 1 827520 828026 OFF 12 YLR349W (827520..828026) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C YLR350W 1 828729 829379 ON 12 ORM2 (828729..829379) Evolutionarily conserved protein with similarity to Orm1p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum YLR351C 0 829488 830363 ON 12 NIT3 complement(829488..830363) Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member YLR352W 1 831115 833538 ON 12 YLR352W (831115..833538) Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene YLR353W 1 834351 836162 ON 12 BUD8 (834351..836162) Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole YLR354C 0 836349 837356 ON 12 TAL1 complement(836349..837356) Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate YLR355C 0 838065 839252 ON 12 ILV5 complement(838065..839252) Acetohydroxyacid reductoisomerase, mitochondrial protein involved in branched-chain amino acid biosynthesis, also required for maintenance of wild-type mitochondrial DNA YLR356W 1 840320 840913 ON 12 YLR356W (840320..840913) Putative protein of unknown function with similarity to SCM4; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR356W is not an essential gene YLR357W 1 841330 843999 ON 12 RSC2 (841330..843999) Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance YLR358C 0 843486 844049 OFF 12 YLR358C complement(843486..844049) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSC2/YLR357W YLR359W 1 844281 845729 ON 12 ADE13 (844281..845729) Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency YLR360W 1 846102 847421 ON 12 VPS38 (846102..847421) Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity YLR361C 0 847387 849123 ON 12 DCR2 complement(847387..849123) Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START YLR361C-A 0 849382 849678 ON 12 YLR361C-A complement(849382..849678) Putative protein of unknown function YLR362W 1 849865 852018 ON 12 STE11 (849865..852018) Signal transducing MEK kinase involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p YLR363C 0 852494 853150 ON 12 NMD4 complement(852494..853150) Protein interacting with Nam7p, may be involved in the nonsense-mediated mRNA decay pathway YLR363W-A 1 853460 853717 ON 12 YLR363W-A (853460..853717) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YLR364W 1 854061 854390 ON 12 YLR364W (854061..854390) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR364W is not an essential gene YLR365W 1 855198 855530 OFF 12 YLR365W (855198..855530) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene YLR364C-A 0 855522 855644 OFF 12 YLR364C-A complement(855522..855644) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR366W YLR366W 1 855537 855842 OFF 12 YLR366W (855537..855842) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A YLR367W 1 856441 857316 ON 12 RPS22B (856441..856573,857057..857316) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Ap and has similarity to E. coli S8 and rat S15a ribosomal proteins YLR368W 1 857539 859335 ON 12 MDM30 (857539..859335) F-box protein; physically associates with mitochondria and is required for normal mitochondrial fusion in rich medium, during sporulation, and in mating cells; promotes ubiquitin-mediated degradation of Gal4p in some strains YLR369W 1 859551 861524 ON 12 SSQ1 (859551..861524) Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia YLR370C 0 861717 862253 ON 12 ARC18 complement(861717..862253) Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches YLR371W 1 862713 866783 ON 12 ROM2 (862713..866783) GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP YLR372W 1 867353 868390 ON 12 SUR4 (867353..868390) Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis YLR373C 0 868661 871366 ON 12 VID22 complement(868661..871366) Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles YLR374C 0 871452 871841 OFF 12 YLR374C complement(871452..871841) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W YLR375W 1 871696 872727 ON 12 STP3 (871696..872727) Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids YLR376C 0 872825 873553 ON 12 PSY3 complement(872825..873553) Protein of unknown function; deletion results in a mutator phenotype suggesting a role for this protein as a mutational suppressor; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C YLR377C 0 873745 874791 ON 12 FBP1 complement(873745..874791) Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism YLR378C 0 875735 877177 ON 12 SEC61 complement(875735..877177) Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER YLR379W 1 876921 877295 OFF 12 YLR379W (876921..877295) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C YLR380W 1 878282 879508 ON 12 CSR1 (878282..879508) Phosphatidylinositol transfer protein with a potential role in lipid turnover; interacts specifically with thioredoxin peroxidase (Tsa2p) and may have a role in oxidative stress resistance YLR381W 1 879723 881924 ON 12 CTF3 (879723..881924) Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules YLR382C 0 882067 884751 ON 12 NAM2 complement(882067..884751) Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance YLR383W 1 885288 888632 ON 12 SMC6 (885288..888632) Protein involved in structural maintenance of chromosomes; essential subunit of Mms21-Smc5-Smc6 complex; required for growth, DNA repair, interchromosomal and sister chromatid recombination; homologous to S. pombe rad18 YLR384C 0 888851 892900 ON 12 IKI3 complement(888851..892900) Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) YLR385C 0 892992 893390 ON 12 SWC7 complement(892992..893390) Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin YLR386W 1 893628 896270 ON 12 VAC14 (893628..896270) Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p YLR387C 0 896374 897672 ON 12 REH1 complement(896374..897672) Protein of unknown function, similar to Rei1p but not involved in bud growth; contains dispersed C2H2 zinc finger domains YLR388W 1 898651 898821 ON 12 RPS29A (898651..898821) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similarity to rat S29 and E. coli S14 ribosomal proteins YLR389C 0 899577 902660 ON 12 STE23 complement(899577..902660) Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; member of the insulin-degrading enzyme family YLR390W 1 903066 903404 ON 12 ECM19 (903066..903404) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR390W-A 1 903724 904440 ON 12 CCW14 (903724..904440) Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall YLR392C 0 904749 906305 ON 12 YLR392C complement(904749..906305) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR392C is not an essential gene YLR393W 1 907079 907918 ON 12 ATP10 (907079..907918) Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 YLR394W 1 907950 909398 ON 12 CST9 (907950..909398) SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate YLR395C 0 909729 909965 ON 12 COX8 complement(909729..909965) Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain YLR396C 0 910235 912310 ON 12 VPS33 complement(910235..912310) ATP-binding protein that is a subunit of the HOPS complex and the CORVET tethering complex; essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport YLR397C 0 912550 914892 ON 12 AFG2 complement(912550..914892) ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; may be involved in degradation of aberrant mRNAs YLR398C 0 915156 919019 ON 12 SKI2 complement(915156..919019) Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses YLR399C 0 919536 921596 ON 12 BDF1 complement(919536..921596) Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p YLR399W-A 1 920869 920973 OFF 12 YLR399W-A (920869..920973) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the characterized ORF BDF1/YLR399C YLR400W 1 922063 922536 OFF 12 YLR400W (922063..922536) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR401C 0 922442 924448 ON 12 DUS3 complement(922442..924448) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region YLR402W 1 924564 925079 OFF 12 YLR402W (924564..925079) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR403W 1 925567 927618 ON 12 SFP1 (925567..927618) Transcription factor that controls expression of many ribosome biogenesis genes in response to nutrients and stress, regulates G2/M transitions during mitotic cell cycle and DNA-damage response, involved in cell size modulation YLR404W 1 928742 929599 ON 12 YLR404W (928742..929599) Putative protein of unknown function; may be an integral membrane protein; YLR404W is not an essential gene YLR405W 1 929788 930891 ON 12 DUS4 (929788..930891) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p YLR406C 0 931064 931754 ON 12 RPL31B complement(931064..931348,931698..931754) Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similarity to rat L31 ribosomal protein; associates with the karyopherin Sxm1p YLR406C-A 0 932205 932354 OFF 12 YLR406C-A complement(932205..932354) Putative protein of unknown function YLR407W 1 932966 933655 ON 12 YLR407W (932966..933655) Putative protein of unknown function; YLR407W is not an essential gene YLR408C 0 933883 934251 ON 12 YLR408C complement(933883..934251) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene YLR409C 0 934412 937231 ON 12 UTP21 complement(934412..937231) Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data YLR410W 1 937539 940979 ON 12 VIP1 (937539..940979) Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of IP7 by Vip1p is important for phosphate signaling; likely involved in cortical actin cytoskeleton function, by analogy with S. pombe ortholog asp1 YLR411W 1 947251 947976 ON 12 CTR3 (947251..947976) High-affinity copper transporter of the plasma membrane, acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae YLR412W 1 948366 949190 ON 12 YLR412W (948366..949190) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene YLR412C-A 0 950264 950470 OFF 12 YLR412C-A complement(950264..950470) Putative protein of unknown function YLR413W 1 951153 953180 ON 12 YLR413W (951153..953180) Putative protein of unknown function; YLR413W is not an essential gene YLR414C 0 953350 954141 ON 12 YLR414C complement(953350..954141) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the bud and cytoplasm; Hog1p is required for transcriptional induction in response to cell wall damage; YLR414C is not an essential gene YLR415C 0 954256 954594 OFF 12 YLR415C complement(954256..954594) Putative protein of unknown function; YLR415C is not an essential gene YLR416C 0 954488 954886 OFF 12 YLR416C complement(954488..954886) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR417W 1 955007 956707 ON 12 VPS36 (955007..956707) Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome YLR418C 0 956911 958092 ON 12 CDC73 complement(956911..958092) Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinct from Srb-containing Pol II complexes; required for expression of certain genes, modification of some histones, and telomere maintenance YLR419W 1 958425 962732 ON 12 YLR419W (958425..962732) Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene YLR420W 1 963782 964876 ON 12 URA4 (963782..964876) Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate YLR421C 0 965087 965557 ON 12 RPN13 complement(965087..965557) Subunit of the 19S regulatory particle of the 26S proteasome lid YLR422W 1 965894 971692 ON 12 YLR422W (965894..971692) Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein YLR423C 0 971914 973167 ON 12 ATG17 complement(971914..973167) Scaffold potein responsible for pre-autophagosomal structure organization; interacts with and is required for activation of Apg1p protein kinase; involved in autophagy but not in the Cvt (cytoplasm to vacuole targeting) pathway YLR424W 1 973392 975518 ON 12 SPP382 (973392..975518) Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation YLR425W 1 982891 986814 ON 12 TUS1 (982891..986814) Guanine nucleotide exchange factor (GEF) that functions to modulate Rho1p activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate YLR426W 1 987059 988110 ON 12 YLR426W (987059..987138,987210..988110) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin YLR427W 1 988425 990437 ON 12 MAG2 (988425..990437) Cytoplasmic protein of unknown function predicted to encode a DNA-3-methyladenine glycosidase II that catalyzes the hydrolysis of alkylated DNA YLR428C 0 990614 990958 OFF 12 YLR428C complement(990614..990958) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 YLR429W 1 990774 992729 ON 12 CRN1 (990774..992729) Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity YLR430W 1 993431 1000126 ON 12 SEN1 (993431..1000126) Putative helicase required for RNA polymerase II transcription termination and processing of RNAs; homolog of Senataxin which causes Ataxia-Oculomotor Apraxia 2 and a dominant form of amyotrophic lateral sclerosis YLR431C 0 1000339 1001700 ON 12 ATG23 complement(1000339..1001700) Peripheral membrane protein, required for autophagy and for the cytoplasm-to-vacuole targeting (Cvt) pathway YLR432W 1 1002554 1004125 ON 12 IMD3 (1002554..1004125) Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed YLR433C 0 1004344 1006005 ON 12 CNA1 complement(1004344..1006005) Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 YLR434C 0 1006022 1006405 OFF 12 YLR434C complement(1006022..1006405) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W YLR435W 1 1006375 1006992 ON 12 TSR2 (1006375..1006992) Protein with a potential role in pre-rRNA processing YLR436C 0 1007418 1011242 ON 12 ECM30 complement(1007418..1011242) Non-essential protein of unknown function YLR437C 0 1011619 1012020 ON 12 YLR437C complement(1011619..1012020) Putative protein of unknown function; epitope tagged protein localizes to the cytoplasm YLR437C-A 0 1012456 1012683 OFF 12 YLR437C-A complement(1012456..1012683) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CAR2/YLR438W YLR438W 1 1012498 1013772 ON 12 CAR2 (1012498..1013772) L-ornithine transaminase (OTAse), catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive YLR438C-A 0 1013906 1014175 ON 12 LSM3 complement(1013906..1014175) Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA YLR439W 1 1014488 1015447 ON 12 MRPL4 (1014488..1015447) Mitochondrial ribosomal protein of the large subunit YLR440C 0 1015565 1017694 ON 12 SEC39 complement(1015565..1017694) Protein of unknown function proposed to be involved in protein secretion YLR441C 0 1018138 1018905 ON 12 RPS1A complement(1018138..1018905) Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein YLR442C 0 1019312 1022248 ON 12 SIR3 complement(1019312..1022248) Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p YLR443W 1 1022622 1023968 ON 12 ECM7 (1022622..1023968) Non-essential protein of unknown function YLR444C 0 1023683 1023985 OFF 12 YLR444C complement(1023683..1023985) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YLR445W 1 1024186 1024834 ON 12 YLR445W (1024186..1024569,1024652..1024834) Hypothetical protein YLR446W 1 1025211 1026512 ON 12 YLR446W (1025211..1026512) Putative protein of unknown function with similarity to hexokinases; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene YLR447C 0 1026853 1027890 ON 12 VMA6 complement(1026853..1027890) Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found in the endomembrane system; stabilizes VO subunits; required for V1 domain assembly on the vacuolar membrane YLR448W 1 1028850 1029764 ON 12 RPL6B (1028850..1028864,1029249..1029764) Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Ap and to rat L6 ribosomal protein; binds to 5.8S rRNA YLR449W 1 1030830 1032008 ON 12 FPR4 (1030830..1032008) Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones YLR450W 1 1032624 1035761 ON 12 HMG2 (1032624..1035761) One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks YLR451W 1 1036090 1038750 ON 12 LEU3 (1036090..1038750) Zinc-finger transcription factor that regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; positively regulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis YLR452C 0 1039268 1041364 ON 12 SST2 complement(1039268..1041364) GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family YLR453C 0 1041797 1042984 ON 12 RIF2 complement(1041797..1042984) Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation YLR454W 1 1043996 1051882 ON 12 YLR454W (1043996..1051882) Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YLR455W 1 1053627 1054541 ON 12 YLR455W (1053627..1054541) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) YLR456W 1 1055068 1055682 ON 12 YLR456W (1055068..1055682) Putative protein of unknown function YLR457C 0 1055810 1056769 ON 12 NBP1 complement(1055810..1056769) Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex YLR458W 1 1056453 1056833 OFF 12 YLR458W (1056453..1056833) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis YLR459W 1 1057332 1058516 ON 12 GAB1 (1057332..1058516) GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI YLR460C 0 1059755 1060885 ON 12 YLR460C complement(1059755..1060885) Putative protein of unknown function, possibly up-regulated by iodine YLR461W 1 1062917 1063279 ON 12 PAU4 (1062917..1063279) Part of 23-member seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme YLR462W 1 1065954 1066562 ON 12 YLR462W (1065954..1066562) Putative protein of unknown function with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII YLR463C 0 1066263 1066814 OFF 12 YLR463C complement(1066263..1066814) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORFs YLR462W and YLR464W YLR464W 1 1066570 1067499 ON 12 YLR464W (1066570..1067082,1067362..1067499) Putative protein of unknown function with similarity to helicases; YLR464W contains an intron and overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C YLR465C 0 1067043 1067351 OFF 12 BSC3 complement(1067043..1067351) Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 100% of YLR465C overlaps the uncharacterized ORF YLR464W and 86% of YLR465C overlaps the verified gene YRF1-4 YLR466W 1 1067085 1071233 ON 12 YRF1-4 (1067085..1071233) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YLR466C-A 0 1070385 1070867 OFF 12 YLR466C-A complement(1070385..1070867) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W YLR466C-B 0 1071592 1071708 OFF 12 YLR466C-B complement(1071592..1071708) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YLR467W 1 1072506 1077896 ON 12 YRF1-5 (1072506..1077896) Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p YLR467C-A 0 1077048 1077530 OFF 12 YLR467C-A complement(1077048..1077530) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YRF1-4/YLR466W YML133C 0 461 4684 ON 13 YML133C complement(461..3791,3891..4684) Putative protein of unknown function with similarity to helicases; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron YML133W-B 1 827 1309 OFF 13 YML133W-B (827..1309) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C YML133W-A 1 1610 2185 OFF 13 YML133W-A (1610..2185) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the uncharacterized ORF YML133C YML132W 1 7244 8383 ON 13 COS3 (7244..8383) Protein involved in salt resistance; interacts with sodium:hydrogen antiporter Nha1p; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins YML131W 1 10199 11296 ON 13 YML131W (10199..11296) Putative protein of unknown function with similarity to oxidoreductases; HOG1 and SKO1-dependent mRNA expression is induced after osmotic shock; GFP-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS YML130C 0 11484 13175 ON 13 ERO1 complement(11484..13175) Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum YML129C 0 14542 14754 ON 13 COX14 complement(14542..14754) Mitochondrial membrane protein, required for assembly of cytochrome c oxidase YML128C 0 15136 16677 ON 13 MSC1 complement(15136..16677) Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies YML127W 1 17065 18810 ON 13 RSC9 (17065..18810) Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway YML126C 0 19061 20536 ON 13 ERG13 complement(19061..20536) 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis YML125C 0 20762 21700 ON 13 PGA3 complement(20762..21700) Essential protein required for maturation of Gas1p and Pho8p, protein trafficking; GFP-fusion protein localizes to the endoplasmic reticulum; null mutants have a cell separation defect YML124C 0 22049 23684 ON 13 TUB3 complement(22049..23361,23660..23684) Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p YML123C 0 24038 25801 ON 13 PHO84 complement(24038..25801) High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p YML122C 0 26039 26419 OFF 13 YML122C complement(26039..26419) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YML121W 1 26930 27862 ON 13 GTR1 (26930..27862) Cytoplasmic GTP binding protein and negative regulator, with homolog Gtr2p, of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport; has homology to human RagA and RagB YML120C 0 28266 29807 ON 13 NDI1 complement(28266..29807) NADH:ubiquinone oxidoreductase, transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; homolog of human AMID YML119W 1 30611 31684 ON 13 YML119W (30611..31684) Putative protein of unknown funtion; YML119W is not an essential gene; potential Cdc28p substrate YML118W 1 32334 33851 ON 13 NGL3 (32334..33851) Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p YML117W 1 34243 37647 ON 13 NAB6 (34243..37647) Putative RNA-binding protein, based on computational analysis of large-scale protein-protein interaction data YML116W-A 1 37472 37774 OFF 13 YML116W-A (37472..37774) Putative protein of unknown function YML116W 1 38196 39824 ON 13 ATR1 (38196..39824) Multidrug efflux pump of the major facilitator superfamily, required for resistance to aminotriazole and 4-nitroquinoline-N-oxide YML115C 0 40187 41794 ON 13 VAN1 complement(40187..41794) Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant YML114C 0 42043 43575 ON 13 TAF8 complement(42043..43575) TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation YML113W 1 44045 44791 ON 13 DAT1 (44045..44791) DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability YML112W 1 45063 45953 ON 13 CTK3 (45063..45953) Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing YML111W 1 46942 49704 ON 13 BUL2 (46942..49704) Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1 YML110C 0 50031 50954 ON 13 COQ5 complement(50031..50954) 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes YML109W 1 51640 54468 ON 13 ZDS2 (51640..54468) Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; paralog of Zds1p YML108W 1 54793 55110 ON 13 YML108W (54793..55110) Putative protein of unknown function whose structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene YML107C 0 55265 56269 ON 13 PML39 complement(55265..56269) Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes YML106W 1 56773 57453 ON 13 URA5 (56773..57453) One of two orotate phosphoribosyltransferase isozymes (see also URA10) that catalyze the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate YML105C 0 57866 58687 ON 13 SEC65 complement(57866..58687) Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 YML104C 0 58939 62322 ON 13 MDM1 complement(58939..62322) Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) YML103C 0 62582 67549 ON 13 NUP188 complement(62582..67549) Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p YML102W 1 68294 69700 ON 13 CAC2 (68294..69700) Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized histones onto recently replicated DNA, required for building functional kinetochores, conserved from yeast to humans YML101C-A 0 69409 69726 OFF 13 YML101C-A complement(69409..69726) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML101C 0 69735 70088 ON 13 CUE4 complement(69735..70088) Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination YML100W-A 1 70138 70311 OFF 13 YML100W-A (70138..70311) Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching YML100W 1 70624 73920 ON 13 TSL1 (70624..73920) Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function YML099W-A 1 74229 74558 OFF 13 YML099W-A (74229..74558) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF ARG81/YML099C YML099C 0 74398 77040 ON 13 ARG81 complement(74398..77040) Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p YML098W 1 77267 77770 ON 13 TAF13 (77267..77770) TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors YML097C 0 78335 79690 ON 13 VPS9 complement(78335..79690) A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin YML096W 1 79909 81486 ON 13 YML096W (79909..81486) Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 YML095C 0 81481 82113 ON 13 RAD10 complement(81481..82113) Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein YML094C-A 0 82219 82620 OFF 13 YML094C-A complement(82219..82620) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO YML094W 1 82275 82849 ON 13 GIM5 (82275..82290,82374..82849) Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it YML093W 1 83090 85789 ON 13 UTP14 (83090..85789) Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA YML092C 0 85987 86739 ON 13 PRE8 complement(85987..86739) 20S proteasome beta-type subunit YML091C 0 87123 90731 ON 13 RPM2 complement(87123..90731) Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus YML090W 1 90744 91130 OFF 13 YML090W (90744..91130) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source YML089C 0 91041 91409 OFF 13 YML089C complement(91041..91409) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage YML088W 1 92235 94241 ON 13 UFO1 (92235..94241) F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation YML087C 0 94431 95369 ON 13 YML087C complement(94431..95369) Putative protein of unknown function YML086C 0 95791 97371 ON 13 ALO1 complement(95791..97371) D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of D-erythroascorbic acid, which is protective against oxidative stress YML085C 0 97941 99400 ON 13 TUB1 complement(97941..99259,99376..99400) Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules YML084W 1 99489 99797 OFF 13 YML084W (99489..99797) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YML083C 0 99794 101050 ON 13 YML083C complement(99794..101050) Putative protein of unknown function; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions YML082W 1 101862 103811 ON 13 YML082W (101862..103811) Putative protein predicted to have carbon-sulfur lyase activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YNML082W is not an essential gene YML081C-A 0 103983 104162 ON 13 ATP18 complement(103983..104162) Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms YML081W 1 104777 108532 ON 13 YML081W (104777..108532) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YML081w is not an essential gene YML080W 1 108806 110077 ON 13 DUS1 (108806..110077) Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 YML079W 1 110247 110852 ON 13 YML079W (110247..110852) Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm YML078W 1 111002 111550 ON 13 CPR3 (111002..111550) Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria YML077W 1 111865 112344 ON 13 BET5 (111865..112344) Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi YML076C 0 112513 115347 ON 13 WAR1 complement(112513..115347) Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively YML075C 0 115734 118898 ON 13 HMG1 complement(115734..118898) One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae YML074C 0 120089 121324 ON 13 FPR3 complement(120089..121324) Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p YML073C 0 123227 124172 ON 13 RPL6A complement(123227..123742,124158..124172) N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Bp and to rat L6 ribosomal protein; binds to 5.8S rRNA YML072C 0 124730 129367 ON 13 TCB3 complement(124730..129367) Lipid-binding protein, localized to the bud via specific mRNA transport; non-tagged protein localizes to mitochondria and GFP-fusion protein localizes to the cell periphery; C-termini of Tcb1p, Tcb2p and Tcb3p interact YML071C 0 129749 131572 ON 13 COG8 complement(129749..131572) Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments YML070W 1 133475 135229 ON 13 DAK1 (133475..135229) Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation YML069W 1 135500 137158 ON 13 POB3 (135500..137158) Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which facilitates RNA Pol II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure; interacts with DNA polymerase alpha (Pol1p) YML068W 1 137550 138944 ON 13 ITT1 (137550..138944) Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins YML067C 0 139063 140214 ON 13 ERV41 complement(139063..140090,140184..140214) Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein YML066C 0 140424 141533 ON 13 SMA2 complement(140424..141533) Protein of unknown function involved in the assembly of the prospore membrane during sporulation YML065W 1 142210 144954 ON 13 ORC1 (142210..144954) Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p YML064C 0 145139 145876 ON 13 TEM1 complement(145139..145876) GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis YML063W 1 146482 147249 ON 13 RPS1B (146482..147249) Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein YML062C 0 147505 148683 ON 13 MFT1 complement(147505..148683) Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance YML061C 0 148953 151532 ON 13 PIF1 complement(148953..151532) DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA YML060W 1 151871 153001 ON 13 OGG1 (151871..153001) Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA YML059C 0 153219 158258 ON 13 NTE1 complement(153219..158258) Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine YML058W-A 1 158760 158966 ON 13 HUG1 (158760..158966) Protein involved in the Mec1p-mediated checkpoint pathway that responds to DNA damage or replication arrest, transcription is induced by DNA damage YML058W 1 159383 159697 ON 13 SML1 (159383..159697) Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase YML057C-A 0 160023 160412 OFF 13 YML057C-A complement(160023..160412) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W YML057W 1 160180 161994 ON 13 CMP2 (160180..161994) Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 YML056C 0 162194 164176 ON 13 IMD4 complement(162194..163308,163717..164176) Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed YML055W 1 164790 165326 ON 13 SPC2 (164790..165326) Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 YML054C 0 165533 167308 ON 13 CYB2 complement(165533..167308) Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions YML054C-A 0 167623 167781 OFF 13 YML054C-A complement(167623..167781) Putative protein of unknown function YML053C 0 169116 169754 ON 13 YML053C complement(169116..169754) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YML053C is not an essential gene YML052W 1 170402 171310 ON 13 SUR7 (170402..171310) Putative integral membrane protein; component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants YML051W 1 171594 172901 ON 13 GAL80 (171594..172901) Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding YML050W 1 173139 174074 ON 13 YML050W (173139..174074) Putative protein of unknown function; non-essential gene YML049C 0 174220 178305 ON 13 RSE1 complement(174220..178305) Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport YML048W 1 178426 179637 ON 13 GSF2 (178426..179637) ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression YML047W-A 1 179893 180258 OFF 13 YML047W-A (179893..180258) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML047C 0 180017 181075 ON 13 PRM6 complement(180017..181075) Pheromone-regulated protein, predicted to have 2 transmembrane segments; regulated by Ste12p during mating YML046W 1 181474 183363 ON 13 PRP39 (181474..183363) U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats YML043C 0 190244 191767 ON 13 RRN11 complement(190244..191767) Protein required for rDNA transcription by RNA polymerase I, component of the core factor (CF) of rDNA transcription factor, which also contains Rrn6p and Rrn7p YML042W 1 192788 194800 ON 13 CAT2 (192788..194800) Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes YML041C 0 194913 195755 ON 13 VPS71 complement(194913..195755) Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting YML038C 0 202775 204103 ON 13 YMD8 complement(202775..204103) Putative nucleotide sugar transporter, has similarity to Vrg4p YML037C 0 204386 205408 ON 13 YML037C complement(204386..205408) Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene YML036W 1 205642 206293 ON 13 CGI121 (205642..206097,206204..206293) Protein involved in telomere uncapping and elongation as component of the KEOPS protein complex with Bud32p, Kae1p, Pcc1p, and Gon7p; also shown to be a component of the EKC protein complex; homolog of human CGI-121 YML035C 0 206428 208860 ON 13 AMD1 complement(206428..208860) AMP deaminase, tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; may be involved in regulation of intracellular adenine nucleotide pools YML034C-A 0 209428 209826 OFF 13 YML034C-A complement(209428..209826) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YML034W 1 209525 212155 ON 13 SRC1 (209525..211444,211571..212155) Protein with a putative role in sister chromatid segregation, potentially phosphorylated by Cdc28p; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery YML032C 0 212515 213930 ON 13 RAD52 complement(212515..213930) Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis YML031C-A 0 214086 214421 OFF 13 YML031C-A complement(214086..214421) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF NDC1/YML031W; questionable ORF from MIPS YML031W 1 214189 216156 ON 13 NDC1 (214189..216156) Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II YML030W 1 216435 216914 ON 13 YML030W (216435..216914) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YML030W is not an essential gene YML029W 1 217362 219878 ON 13 USA1 (217362..219878) Protein involved in ER-associated protein degradation (ERAD); component of the Hrd1p complex; interacts with the U1 snRNP-specific protein, Snp1p YML028W 1 220138 220728 ON 13 TSA1 (220138..220728) Ubiquitous housekeeping thioredoxin peroxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; mediates redox regulation of the nuclear localization of Yap1p; deletion results in mutator phenotype YML027W 1 221406 222563 ON 13 YOX1 (221406..222563) Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate YML026C 0 222987 223828 ON 13 RPS18B complement(222987..223380,223782..223828) Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similarity to E. coli S13 and rat S18 ribosomal proteins YML025C 0 224406 225365 ON 13 YML6 complement(224406..225239,225339..225365) Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins YML024W 1 225889 226697 ON 13 RPS17A (225889..225891,226290..226697) Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similarity to rat S17 ribosomal protein YML023C 0 226994 228664 ON 13 NSE5 complement(226994..228664) Essential subunit of the Mms21-Smc5-Smc6 complex; required for cell viability and DNA repair YML022W 1 228937 229500 ON 13 APT1 (228937..229500) Adenine phosphoribosyltransferase, catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis YML021C 0 229734 230813 ON 13 UNG1 complement(229734..230813) Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus YML020W 1 231149 233143 ON 13 YML020W (231149..233143) Putative protein of unknown function YML019W 1 233457 234455 ON 13 OST6 (233457..234455) Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p YML018C 0 234771 235952 ON 13 YML018C complement(234771..235952) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; YML018C is not an essential gene YML017W 1 236588 238731 ON 13 PSP2 (236588..236591,236954..238731) Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing YML016C 0 239458 241536 ON 13 PPZ1 complement(239458..241536) Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance YML015C 0 241989 243029 ON 13 TAF11 complement(241989..243029) TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors YML014W 1 243225 244064 ON 13 TRM9 (243225..244064) tRNA methyltransferase, catalyzes the esterification of modified uridine nucleotides in tRNAs, creating 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu); may have a role in stress response YML013W 1 244149 245903 ON 13 UBX2 (244149..245903) Protein involved in ER-associated protein degradation; proposed to coordinate the assembly of proteins involved in ERAD; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain YML012C-A 0 245537 245914 OFF 13 YML012C-A complement(245537..245914) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 YML012W 1 246116 246751 ON 13 ERV25 (246116..246751) Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport YML011C 0 246895 247428 ON 13 RAD33 complement(246895..247428) Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus YML010W 1 247677 250868 ON 13 SPT5 (247677..250868) Protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation; Spt4p-Spt5p complex also plays a role in pre-mRNA processing YML009W-B 1 250435 250911 OFF 13 YML009W-B (250435..250911) Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data YML009C-A 0 250675 251001 OFF 13 YML009C-A complement(250675..251001) Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data YML009C 0 251304 251516 ON 13 MRPL39 complement(251304..251516) Mitochondrial ribosomal protein of the large subunit YML008C 0 251839 252990 ON 13 ERG6 complement(251839..252990) Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane YML007C-A 0 253162 253272 OFF 13 YML007C-A complement(253162..253272) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria YML007W 1 253848 255800 ON 13 YAP1 (253848..255800) Basic leucine zipper (bZIP) transcription factor required for oxidative stress tolerance; mediates pleiotropic drug and metal resistance; localized to the nucleus in response to the presence of oxidants YML006C 0 256092 258416 ON 13 GIS4 complement(256092..258416) CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway YML005W 1 260221 261609 ON 13 TRM12 (260221..261609) S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA YML004C 0 261705 262685 ON 13 GLO1 complement(261705..262685) Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress YML003W 1 263483 264355 OFF 13 YML003W (263483..264355) Putative protein of unknown function YML002W 1 264541 266754 ON 13 YML002W (264541..266754) Putative protein of unknown function; expression induced by heat and by calcium shortage YML001W 1 267174 267800 ON 13 YPT7 (267174..267800) GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion, similar to mammalian Rab7 YMR001C 0 269019 271136 ON 13 CDC5 complement(269019..271136) Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate YMR001C-A 0 271347 271577 OFF 13 YMR001C-A complement(271347..271577) Putative protein of unknown function YMR002W 1 272193 272663 ON 13 MIC17 (272193..272663) Mitochondrial intermembrane space cysteine motif protein; MIC17 is not an essential gene YMR003W 1 273118 273714 ON 13 YMR003W (273118..273714) Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria; YMR003W is not an essential gene YMR004W 1 274017 275552 ON 13 MVP1 (274017..275552) Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole YMR005W 1 276045 277211 ON 13 TAF4 (276045..277211) TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate YMR006C 0 277561 279681 ON 13 PLB2 complement(277561..279681) Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine YMR007W 1 279960 280340 OFF 13 YMR007W (279960..280340) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data YMR008C 0 280590 282584 ON 13 PLB1 complement(280590..282584) Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol YMR009W 1 284101 284640 ON 13 ADI1 (284101..284640) Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions YMR010W 1 285099 286316 ON 13 YMR010W (285099..286316) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 YMR011W 1 288078 289703 ON 13 HXT2 (288078..289703) High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose YMR012W 1 291133 294966 ON 13 CLU1 (291133..294966) eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant YMR013C 0 295178 296737 ON 13 SEC59 complement(295178..296737) Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation YMR013C-A 0 296470 296619 OFF 13 YMR013C-A complement(296470..296619) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF SEC59/YML013C YMR013W-A 1 298310 298390 OFF 13 YMR013W-A (298310..298390) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 YMR014W 1 298867 300426 ON 13 BUD22 (298867..300426) Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YMR015C 0 300868 302484 ON 13 ERG5 complement(300868..302484) C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs YMR016C 0 303235 305592 ON 13 SOK2 complement(303235..305592) Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; negatively regulates pseudohyphal differentiation; homologous to several transcription factors YMR017W 1 307488 308681 ON 13 SPO20 (307488..308681) Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog YMR018W 1 310207 311751 ON 13 YMR018W (310207..311751) Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene YMR019W 1 312155 315004 ON 13 STB4 (312155..315004) Protein that binds Sin3p in a two-hybrid assay; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins YMR020W 1 315376 316902 ON 13 FMS1 (315376..316902) Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis YMR021C 0 317164 318417 ON 13 MAC1 complement(317164..318417) Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport YMR022W 1 318679 319176 ON 13 UBC7 (318679..319176) Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly YMR023C 0 319436 321016 ON 13 MSS1 complement(319436..321016) Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 YMR024W 1 321874 323046 ON 13 MRPL3 (321874..323046) Mitochondrial ribosomal protein of the large subunit YMR025W 1 323299 324186 ON 13 CSI1 (323299..324186) Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling YMR026C 0 324235 325434 ON 13 PEX12 complement(324235..325434) C3HC4-type RING-finger peroxisomal membrane peroxin required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorders YMR027W 1 325876 327288 ON 13 YMR027W (325876..327288) Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene YMR028W 1 327481 328581 ON 13 TAP42 (327481..328581) Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits YMR029C 0 328659 330230 ON 13 FAR8 complement(328659..330230) Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p YMR030W 1 330792 331922 ON 13 RSF1 (330792..331922) Protein required for respiratory growth; localized to both the nucleus and mitochondrion; mutant displays decreased transcription of specific nuclear and mitochondrial genes whose products are involved in respiratory growth YMR031C 0 332211 334742 ON 13 YMR031C complement(332211..334742) Putative protein of unknown function with similarity to YKL050C and USO1/YDL058W; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YMR031C is not an essential gene YMR031W-A 1 334708 335034 OFF 13 YMR031W-A (334708..335034) Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C YMR032W 1 335297 337306 ON 13 HOF1 (335297..337306) Bud neck-localized, SH3 domain-containing protein required for cytokinesis; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p YMR030W-A 1 337312 337602 OFF 13 YMR030W-A (337312..337602) Putative protein of unknown function YMR033W 1 337787 339276 ON 13 ARP9 (337787..337816,337903..339276) Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation YMR034C 0 339417 340721 ON 13 YMR034C complement(339417..340721) Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene YMR035W 1 341141 341674 ON 13 IMP2 (341141..341674) Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p YMR036C 0 341855 343519 ON 13 MIH1 complement(341855..343519) Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 YMR037C 0 344402 346516 ON 13 MSN2 complement(344402..346516) Transcriptional activator related to Msn4p; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression YMR038C 0 347510 348259 ON 13 CCS1 complement(347510..348259) Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation YMR039C 0 348643 349521 ON 13 SUB1 complement(348643..349521) Transcriptional coactivator, facilitates elongation by influencing enzymes that modify RNAP II, acts in a peroxide resistance pathway involving Rad2p; suppressor of TFIIB mutations YMR040W 1 350380 350862 ON 13 YET2 (350380..350862) Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein YMR041C 0 350965 351972 ON 13 ARA2 complement(350965..351972) NAD-dependent arabinose dehydrogenase, involved in biosynthesis of erythroascorbic acid; similar to plant L-galactose dehydrogenase YMR042W 1 352602 353135 ON 13 ARG80 (352602..353135) Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p YMR043W 1 353870 354730 ON 13 MCM1 (353870..354730) Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes YMR044W 1 355383 356810 ON 13 IO