Re-run this search with the SEG filter switched off

Re-run this search as BLASTX i.e. nucleotide query

Get selected genes'

Hit NameAncStatusLength (aa)HSP LengthHSP ScoreHSP E-value
Smik_6.468singletonON26261283e-12
Skud_16.17singletonON5924818e-05
YPL261CsingletonOFF10224812e-04
BLASTP 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Smik_6.468
         (26 letters)

Database: Seq/AA.fsa 
           114,666 sequences; 53,481,399 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Smik_6.468 Chr6 (771911..771916,771920..771991) [78 bp, 26 aa] {...    54   3e-12
Skud_16.17 Chr16 complement(28178..28243,28247..28342,28346..283...    36   8e-05
YPL261C Chr16 complement(48996..49304) [309 bp, 102 aa] {OFF} Du...    36   2e-04

>Smik_6.468 Chr6 (771911..771916,771920..771991) [78 bp, 26 aa]
          {ON} YPL261C (REAL)
          Length = 26

 Score = 53.9 bits (128), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 26/26 (100%), Positives = 26/26 (100%)

Query: 1  KNKSSRRFNDSFPLMLIVLKPIFAAQ 26
          KNKSSRRFNDSFPLMLIVLKPIFAAQ
Sbjct: 1  KNKSSRRFNDSFPLMLIVLKPIFAAQ 26

>Skud_16.17 Chr16
          complement(28178..28243,28247..28342,28346..28360) [177
          bp, 59 aa] {ON} YPL261C (REAL)
          Length = 59

 Score = 35.8 bits (81), Expect = 8e-05,   Method: Compositional matrix adjust.
 Identities = 16/24 (66%), Positives = 19/24 (79%)

Query: 3  KSSRRFNDSFPLMLIVLKPIFAAQ 26
          KS RR N SFPL+L +L+PIFAA 
Sbjct: 15 KSPRRLNSSFPLLLAILEPIFAAS 38

>YPL261C Chr16 complement(48996..49304) [309 bp, 102 aa] {OFF}
          Dubious open reading frame unlikely to encode a
          protein; partially overlaps the uncharacterized ORF
          YPL260W
          Length = 102

 Score = 35.8 bits (81), Expect = 2e-04,   Method: Compositional matrix adjust.
 Identities = 16/24 (66%), Positives = 20/24 (83%)

Query: 3  KSSRRFNDSFPLMLIVLKPIFAAQ 26
          +S RRF+ SFPL+L V KPIFAA+
Sbjct: 20 ESPRRFSSSFPLLLFVFKPIFAAK 43

  Database: Seq/AA.fsa
    Posted date:  Aug 24, 2012  3:22 PM
  Number of letters in database: 53,481,399
  Number of sequences in database:  114,666
  
Lambda     K      H
   0.328    0.138    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 114666
Number of Hits to DB: 2,368,474
Number of extensions: 28080
Number of successful extensions: 77
Number of sequences better than 10.0: 3
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 3
Length of query: 26
Length of database: 53,481,399
Length adjustment: 1
Effective length of query: 25
Effective length of database: 53,366,733
Effective search space: 1334168325
Effective search space used: 1334168325
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 59 (27.3 bits)