Re-run this search with the SEG filter switched off

Re-run this search as BLASTX i.e. nucleotide query

Get selected genes'

Hit NameAncStatusLength (aa)HSP LengthHSP ScoreHSP E-value
Smik_2.1353.187ON127361272e-09
YBR016W3.187ON128361106e-07
Suva_2.1443.187ON119361062e-06
Skud_2.1283.187ON127361052e-06
CAGL0I04510g3.187ON11626860.001
Skud_4.2413.187ON10625750.041
YDL012C3.187ON10725730.070
KAFR0C008803.187ON12125700.23
ZYRO0A04972g3.187ON12227690.35
Kwal_YGOB_Anc_3.1873.187ON11234680.43
KLTH0E15840g3.187ON11325680.48
SAKL0C08008g3.187ON14527670.68
Suva_4.2373.187ON10325622.8
Ecym_52633.187ON12429605.6
KNAG0K012853.187ON12627597.1
NOTE: 9 genes in the same pillar as Smik_2.135 were not hit in these BLAST results
LIST: Kpol_1036.51 CAGL0K01903g Smik_4.222 NCAS0I01900 TBLA0B01680 TBLA0F00730 AER212W TDEL0D04340 Klac_YGOB_Anc_3.187

BLASTP 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Smik_2.135
         (127 letters)

Database: Seq/AA.fsa 
           114,666 sequences; 53,481,399 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Smik_2.135 Chr2 (250473..250856) [384 bp, 127 aa] {ON} YBR016W (...    54   2e-09
YBR016W Chr2 (270247..270633) [387 bp, 128 aa] {ON} Tail-anchore...    47   6e-07
Suva_2.144 Chr2 (249704..250063) [360 bp, 119 aa] {ON} YBR016W (...    45   2e-06
Skud_2.128 Chr2 (243786..244169) [384 bp, 127 aa] {ON} YBR016W (...    45   2e-06
CAGL0I04510g Chr9 complement(403225..403527,403675..403722) [351...    38   0.001
Skud_4.241 Chr4 complement(425844..426119,426211..426255) [321 b...    33   0.041
YDL012C Chr4 complement(431108..431386,431473..431517) [324 bp, ...    33   0.070
KAFR0C00880 Chr3 (178337..178375,178475..178801) [366 bp, 121 aa...    32   0.23 
ZYRO0A04972g Chr1 (400197..400235,400324..400653) [369 bp, 122 a...    31   0.35 
Kwal_YGOB_Anc_3.187 s27 complement(77867..78157,78226..78273) [3...    31   0.43 
KLTH0E15840g Chr5 (1406256..1406303,1406400..1406693) [342 bp, 1...    31   0.48 
SAKL0C08008g Chr3 complement(743966..744268,744380..744514) [438...    30   0.68 
Suva_4.237 Chr4 complement(424387..424653,424741..424785) [312 b...    28   2.8  
Ecym_5263 Chr5 complement(545614..545943,546179..546223) [375 bp...    28   5.6  
KNAG0K01285 Chr11 complement(253589..253930,254081..254119) [381...    27   7.1  

>Smik_2.135 Chr2 (250473..250856) [384 bp, 127 aa] {ON} YBR016W
          (REAL)
          Length = 127

 Score = 53.5 bits (127), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 36/36 (100%), Positives = 36/36 (100%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER 36
          MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER
Sbjct: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER 36

>YBR016W Chr2 (270247..270633) [387 bp, 128 aa] {ON} Tail-anchored
          plasma membrane protein containing a conserved CYSTM
          module; predicted to be palmitoylated; has similarity
          to hydrophilins, which are involved in the adaptive
          response to hyperosmotic conditions
          Length = 128

 Score = 47.0 bits (110), Expect = 6e-07,   Method: Compositional matrix adjust.
 Identities = 33/36 (91%), Positives = 34/36 (94%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER 36
          MSANDYYGG  GEKSQYSRPSNPPPSSAHQN+TQER
Sbjct: 1  MSANDYYGGTAGEKSQYSRPSNPPPSSAHQNKTQER 36

>Suva_2.144 Chr2 (249704..250063) [360 bp, 119 aa] {ON} YBR016W
          (REAL)
          Length = 119

 Score = 45.4 bits (106), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 30/36 (83%), Positives = 31/36 (86%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER 36
          MSANDYYGG  GEK+QYSRPSNPPPSS HQ  TQER
Sbjct: 1  MSANDYYGGTAGEKAQYSRPSNPPPSSTHQGSTQER 36

>Skud_2.128 Chr2 (243786..244169) [384 bp, 127 aa] {ON} YBR016W
          (REAL)
          Length = 127

 Score = 45.1 bits (105), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 32/36 (88%), Positives = 34/36 (94%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQER 36
          MSANDYYGGA GEKSQYSRPSNPPPSSA Q++TQER
Sbjct: 1  MSANDYYGGAAGEKSQYSRPSNPPPSSAQQDKTQER 36

>CAGL0I04510g Chr9 complement(403225..403527,403675..403722) [351
          bp, 116 aa] {ON} similar to uniprot|P38216
          Saccharomyces cerevisiae YBR016w and similar to
          uniprot|Q12489 Saccharomyces cerevisiae YDL012c
          Length = 116

 Score = 37.7 bits (86), Expect = 0.001,   Method: Compositional matrix adjust.
 Identities = 17/26 (65%), Positives = 19/26 (73%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPS 26
          MSA +YY GA   K QYSRP+ PPPS
Sbjct: 1  MSAQEYYNGAGDSKQQYSRPTAPPPS 26

>Skud_4.241 Chr4 complement(425844..426119,426211..426255) [321
          bp, 106 aa] {ON} YDL012C (REAL)
          Length = 106

 Score = 33.5 bits (75), Expect = 0.041,   Method: Compositional matrix adjust.
 Identities = 16/25 (64%), Positives = 20/25 (80%), Gaps = 1/25 (4%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPP 25
          MSA DYYG +T ++S YSRP+ PPP
Sbjct: 1  MSAQDYYGNSTSKQS-YSRPTAPPP 24

>YDL012C Chr4 complement(431108..431386,431473..431517) [324 bp,
          107 aa] {ON} Tail-anchored plasma membrane protein
          containing a conserved CYSTM module, possibly involved
          in response to stress; may contribute to non-homologous
          end-joining (NHEJ) based on ydl012c htz1 double null
          phenotype
          Length = 107

 Score = 32.7 bits (73), Expect = 0.070,   Method: Compositional matrix adjust.
 Identities = 16/25 (64%), Positives = 19/25 (76%), Gaps = 1/25 (4%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPP 25
          MSA DYYG +  ++S YSRPS PPP
Sbjct: 1  MSAQDYYGNSASKQS-YSRPSAPPP 24

>KAFR0C00880 Chr3 (178337..178375,178475..178801) [366 bp, 121 aa]
          {ON} Anc_3.187 YDL012C
          Length = 121

 Score = 31.6 bits (70), Expect = 0.23,   Method: Compositional matrix adjust.
 Identities = 15/25 (60%), Positives = 18/25 (72%), Gaps = 3/25 (12%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPP 25
          MSA +YYG     K+QY+RPS PPP
Sbjct: 1  MSAREYYGDT---KTQYNRPSAPPP 22

>ZYRO0A04972g Chr1 (400197..400235,400324..400653) [369 bp, 122
          aa] {ON} weakly similar to uniprot|P38216 Saccharomyces
          cerevisiae YBR016W
          Length = 122

 Score = 31.2 bits (69), Expect = 0.35,   Method: Compositional matrix adjust.
 Identities = 16/27 (59%), Positives = 19/27 (70%), Gaps = 3/27 (11%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSS 27
          MSA +YYG +   K+ YSRP  PPPSS
Sbjct: 1  MSAQEYYGDS---KASYSRPQGPPPSS 24

>Kwal_YGOB_Anc_3.187 s27 complement(77867..78157,78226..78273)
          [339 bp, 112 aa] {ON} ANNOTATED BY YGOB -
          Length = 112

 Score = 30.8 bits (68), Expect = 0.43,   Method: Compositional matrix adjust.
 Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 2/34 (5%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPPSSAHQNETQ 34
          MSA +YY G +  + QY+ PS PPP   HQ +++
Sbjct: 1  MSAAEYYKGTSQPQQQYTSPSGPPP--GHQQDSR 32

>KLTH0E15840g Chr5 (1406256..1406303,1406400..1406693) [342 bp,
          113 aa] {ON} similar to uniprot|Q12489 Saccharomyces
          cerevisiae YDL012C Plasma membrane protein of unknown
          function
          Length = 113

 Score = 30.8 bits (68), Expect = 0.48,   Method: Compositional matrix adjust.
 Identities = 13/25 (52%), Positives = 17/25 (68%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPP 25
          MSA +YY G +  K QY+ P+ PPP
Sbjct: 1  MSAAEYYKGTSESKQQYTSPAGPPP 25

>SAKL0C08008g Chr3 complement(743966..744268,744380..744514) [438
          bp, 145 aa] {ON} similar to uniprot|Q12489
          Saccharomyces cerevisiae YDL012C Plasma membrane
          protein of unknown function
          Length = 145

 Score = 30.4 bits (67), Expect = 0.68,   Method: Compositional matrix adjust.
 Identities = 15/27 (55%), Positives = 17/27 (62%), Gaps = 1/27 (3%)

Query: 1  MSANDYYGGATGEKSQ-YSRPSNPPPS 26
          MSA DYY GA  +  Q Y RP+  PPS
Sbjct: 29 MSAADYYSGAAADSKQTYQRPTGAPPS 55

>Suva_4.237 Chr4 complement(424387..424653,424741..424785) [312
          bp, 103 aa] {ON} YDL012C (REAL)
          Length = 103

 Score = 28.5 bits (62), Expect = 2.8,   Method: Compositional matrix adjust.
 Identities = 14/25 (56%), Positives = 16/25 (64%), Gaps = 1/25 (4%)

Query: 1  MSANDYYGGATGEKSQYSRPSNPPP 25
          MSA DYYG  +  K  YS P+ PPP
Sbjct: 1  MSAQDYYGN-SAYKQSYSHPTAPPP 24

>Ecym_5263 Chr5 complement(545614..545943,546179..546223) [375 bp,
          124 aa] {ON} similar to Ashbya gossypii AER212W
          Length = 124

 Score = 27.7 bits (60), Expect = 5.6,   Method: Compositional matrix adjust.
 Identities = 16/29 (55%), Positives = 21/29 (72%), Gaps = 3/29 (10%)

Query: 1  MSANDYYGGATGEKSQ-YSRPSNPPPSSA 28
          MSA DYYG  TG+  Q ++RPS PPP+ +
Sbjct: 1  MSAADYYG--TGDNKQSFNRPSAPPPNQS 27

>KNAG0K01285 Chr11 complement(253589..253930,254081..254119) [381
          bp, 126 aa] {ON}
          Length = 126

 Score = 27.3 bits (59), Expect = 7.1,   Method: Compositional matrix adjust.
 Identities = 14/27 (51%), Positives = 19/27 (70%), Gaps = 1/27 (3%)

Query: 1  MSANDYYGGATGEKS-QYSRPSNPPPS 26
          MSA DYY GA+ + + QY  PS+P P+
Sbjct: 1  MSAKDYYNGASNQPTQQYHSPSSPAPA 27

  Database: Seq/AA.fsa
    Posted date:  Aug 24, 2012  3:22 PM
  Number of letters in database: 53,481,399
  Number of sequences in database:  114,666
  
Lambda     K      H
   0.303    0.122    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 114666
Number of Hits to DB: 5,542,747
Number of extensions: 112849
Number of successful extensions: 338
Number of sequences better than 10.0: 17
Number of HSP's gapped: 333
Number of HSP's successfully gapped: 17
Length of query: 127
Length of database: 53,481,399
Length adjustment: 96
Effective length of query: 31
Effective length of database: 42,473,463
Effective search space: 1316677353
Effective search space used: 1316677353
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.8 bits)
S2: 59 (27.3 bits)