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Re-run this search as BLASTX i.e. nucleotide query

Get selected genes'

Hit NameAncStatusLength (aa)HSP LengthHSP ScoreHSP E-value
KLTH0E00924gsingletonON1241245213e-69
BLASTP 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= KLTH0E00924g
         (124 letters)

Database: Seq/AA.fsa 
           114,666 sequences; 53,481,399 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KLTH0E00924g Chr5 (90490..90864) [375 bp, 124 aa] {ON} no simila...   205   3e-69

>KLTH0E00924g Chr5 (90490..90864) [375 bp, 124 aa] {ON} no
           similarity
          Length = 124

 Score =  205 bits (521), Expect = 3e-69,   Method: Compositional matrix adjust.
 Identities = 106/124 (85%), Positives = 106/124 (85%)

Query: 1   MELIKGRQGLRQVLCHSHGVVAPATDTRTSPRAPQSSVGSDKGFEIGSDADFLCQCLLLF 60
           MELIKGRQGLRQVLCHSHGVVAPATDTRTSPRAPQSSVGSDKGFEIGSDADFLCQCLLLF
Sbjct: 1   MELIKGRQGLRQVLCHSHGVVAPATDTRTSPRAPQSSVGSDKGFEIGSDADFLCQCLLLF 60

Query: 61  SAGGLQAQRDALVAQALKTVPFDVTSEEAVVAYASSCXXXXXXXXXXXXXXXXXXAQPDR 120
           SAGGLQAQRDALVAQALKTVPFDVTSEEAVVAYASSC                  AQPDR
Sbjct: 61  SAGGLQAQRDALVAQALKTVPFDVTSEEAVVAYASSCAFGAIGPFGPGGRRLGLRAQPDR 120

Query: 121 SAQR 124
           SAQR
Sbjct: 121 SAQR 124

  Database: Seq/AA.fsa
    Posted date:  Aug 24, 2012  3:22 PM
  Number of letters in database: 53,481,399
  Number of sequences in database:  114,666
  
Lambda     K      H
   0.318    0.130    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 114666
Number of Hits to DB: 8,935,360
Number of extensions: 282353
Number of successful extensions: 530
Number of sequences better than 10.0: 1
Number of HSP's gapped: 529
Number of HSP's successfully gapped: 1
Length of query: 124
Length of database: 53,481,399
Length adjustment: 93
Effective length of query: 31
Effective length of database: 42,817,461
Effective search space: 1327341291
Effective search space used: 1327341291
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)