Re-run this search with the SEG filter switched off

Re-run this search as BLASTX i.e. nucleotide query

Get selected genes'

Hit NameAncStatusLength (aa)HSP LengthHSP ScoreHSP E-value
KLLA0D12452g7.345ON114853163e-38
NOTE: 16 genes in the same pillar as KLLA0D12452g were not hit in these BLAST results
LIST: Kpol_2001.72 Zrou_YGOB_Anc_7.345 KAFR0L01475 Skud_12.267 CAGL0A03883g Smik_12.262 YLR204W Suva_10.298 KLTH0H01364g Kwal_56.24628 ACL153C Ecym_4728 TPHA0D04575 KNAG0B02635 NDAI0E04490 SAKL0F11264g

BLASTP 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= KLLA0D12452g
         (114 letters)

Database: Seq/AA.fsa 
           114,666 sequences; 53,481,399 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KLLA0D12452g Chr4 (1057600..1057944) [345 bp, 114 aa] {ON} some ...   126   3e-38

>KLLA0D12452g Chr4 (1057600..1057944) [345 bp, 114 aa] {ON} some
          similarities with uniprot|P32344 Saccharomyces
          cerevisiae YLR204W QRI5
          Length = 114

 Score =  126 bits (316), Expect = 3e-38,   Method: Compositional matrix adjust.
 Identities = 65/85 (76%), Positives = 65/85 (76%)

Query: 1  MLRSITTRLTSPISGSLRCLSVACCNTQXXXXXXXXXXXXXXXXXXXXTGSLGLPVQENN 60
          MLRSITTRLTSPISGSLRCLSVACCNTQ                    TGSLGLPVQENN
Sbjct: 1  MLRSITTRLTSPISGSLRCLSVACCNTQMAVPMGMPMGMPIGMTAGIPTGSLGLPVQENN 60

Query: 61 HMTEPIVSITDLIKKQEEYRMDSVM 85
          HMTEPIVSITDLIKKQEEYRMDSVM
Sbjct: 61 HMTEPIVSITDLIKKQEEYRMDSVM 85

  Database: Seq/AA.fsa
    Posted date:  Aug 24, 2012  3:22 PM
  Number of letters in database: 53,481,399
  Number of sequences in database:  114,666
  
Lambda     K      H
   0.316    0.128    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 114666
Number of Hits to DB: 6,467,132
Number of extensions: 138846
Number of successful extensions: 333
Number of sequences better than 10.0: 1
Number of HSP's gapped: 332
Number of HSP's successfully gapped: 1
Length of query: 114
Length of database: 53,481,399
Length adjustment: 84
Effective length of query: 30
Effective length of database: 43,849,455
Effective search space: 1315483650
Effective search space used: 1315483650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)