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Re-run this search as BLASTX i.e. nucleotide query

Get selected genes'

Hit NameAncStatusLength (aa)HSP LengthHSP ScoreHSP E-value
AFR740WsingletonON1701708871e-123
BLASTP 2.2.26 [Sep-21-2011]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= AFR740W
         (170 letters)

Database: Seq/AA.fsa 
           114,666 sequences; 53,481,399 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFR740W Chr6 (1797575..1798087) [513 bp, 170 aa] {ON} NOHBY666; ...   346   e-123

>AFR740W Chr6 (1797575..1798087) [513 bp, 170 aa] {ON} NOHBY666; No
           homolog in Saccharomyces cerevisiae
          Length = 170

 Score =  346 bits (887), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 170/170 (100%), Positives = 170/170 (100%)

Query: 1   MGLQTLRASHKPPNKPGHHFRSTQKISFAKLGLLARDRPALLAHKRSFRTVMMISGLCTN 60
           MGLQTLRASHKPPNKPGHHFRSTQKISFAKLGLLARDRPALLAHKRSFRTVMMISGLCTN
Sbjct: 1   MGLQTLRASHKPPNKPGHHFRSTQKISFAKLGLLARDRPALLAHKRSFRTVMMISGLCTN 60

Query: 61  AKEHRLRFSVPDLRAMLVTCGPGIRLDGDQRTQCAHRAPLPAAHGVAKWAPARPLSSRIA 120
           AKEHRLRFSVPDLRAMLVTCGPGIRLDGDQRTQCAHRAPLPAAHGVAKWAPARPLSSRIA
Sbjct: 61  AKEHRLRFSVPDLRAMLVTCGPGIRLDGDQRTQCAHRAPLPAAHGVAKWAPARPLSSRIA 120

Query: 121 YMRCSNASNCPPGSGRISSAFHLHWRFCGLSSVGRVEEGRVSFFLRKLLG 170
           YMRCSNASNCPPGSGRISSAFHLHWRFCGLSSVGRVEEGRVSFFLRKLLG
Sbjct: 121 YMRCSNASNCPPGSGRISSAFHLHWRFCGLSSVGRVEEGRVSFFLRKLLG 170

  Database: Seq/AA.fsa
    Posted date:  Aug 24, 2012  3:22 PM
  Number of letters in database: 53,481,399
  Number of sequences in database:  114,666
  
Lambda     K      H
   0.326    0.137    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 114666
Number of Hits to DB: 15,636,594
Number of extensions: 517597
Number of successful extensions: 974
Number of sequences better than 10.0: 1
Number of HSP's gapped: 981
Number of HSP's successfully gapped: 1
Length of query: 170
Length of database: 53,481,399
Length adjustment: 102
Effective length of query: 68
Effective length of database: 41,785,467
Effective search space: 2841411756
Effective search space used: 2841411756
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)